CN113817691A

CN113817691A - 一种小麦ACCase突变型基因及其在植物抗除草剂中的应用

Info

Publication number: CN113817691A
Application number: CN202111120177.1A
Authority: CN
Inventors: 王金彦; 张保龙; 凌溪铁; 杨郁文; 郭冬姝; 刘廷利
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2018-11-28
Filing date: 2018-11-28
Publication date: 2021-12-21
Anticipated expiration: 2038-11-28
Also published as: CN109355264A; CN109355264B

Abstract

本发明公开了一种小麦ACCase突变型基因及其在植物抗除草剂中的应用，所述ACCase突变型基因编码蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于野生型小麦ACCase的氨基酸序列的第1708位发生突变、第1768位发生突变、第1769位发生突变、第1992位发生突变、第2015位发生突变、第2029位发生突变、第2066位发生突变、第2084位发生突变。植物表达该突变蛋白可以抗乙酰辅酶A羧化酶抑制剂类除草剂。该ACCase突变型基因可广泛应用于植物抗除草剂育种。

Description

一种小麦ACCase突变型基因及其在植物抗除草剂中的应用

技术领域

本发明涉及一种小麦ACCase突变型基因及其在植物抗除草剂中的应用，属于植物基因工程和植物抗除草剂育种领域，该基因所编码的蛋白的氨基酸序列对应于野生型小麦ACCase的氨基酸序列存在以下任意一种或多种突变：对应于野生型小麦ACCase的氨基酸序列的第1708位发生突变、第1768位发生突变、第1769位发生突变、第1992位发生突变、第2015位发生突变、第2029位发生突变、第2066位发生突变、第2084位发生突变。

背景技术

乙酰辅酶A羧化酶(ACCase)类抑制剂是超高效的除草剂，对禾本科杂草有优异的防除效果，而对双子叶植物高度安全，故目前在阔叶作物大田中使用最为普遍。但同时发现长期单一使用该类除草剂，其用量普遍都会逐年增加，药效降低，也就是说，敏感性杂草大量减少，而非敏感性的耐药性杂草逐渐上升演化为群落优势种，并易诱导抗药性杂草的产生。

ACCase同乙酰乳酸合成酶(ALS)和多酚氧化酶(PPO)一样是高效除草剂的靶标酶，针对该靶标的除草剂主要有芳氧苯氧基丙酸类(AOPP)和环己烯酮类(CHD)除草剂，它们对双子叶植物有高度的选择性，而且具有低的哺乳动物毒性和良好的环境特性，受到越来越多的关注。AOPP类除草剂是一类著名的防除禾本科杂草的高活性除草剂，也是一类发展比较迅速、不断开发出新品种的除草剂类型。该类除草剂最早是在20世纪60年代开始研究。目前商品化的AOPP类除草剂有禾草灵、稳杀得、高效盖草能、恶草醚等。该类除草剂存在立体异构，而且这种异构体中只有右旋体对ACCase有显著活性。当用盖草能处理后，植物脂肪氧合酶活性增高，不饱和脂肪酸氧化加剧，自由基对膜的伤害加深；施加各种外源脂氧合酶抑制剂和清除自由基物质可逆转禾草灵和盖草能的毒害作用，而且使禾草灵诱导乙烯形成的作用得到抑制。

由于化学除草剂的广泛使用，杂草抗性发生的速度如此之快、范围如此之广，不但增加了除草成本，也增加了环境压力。目前，对抗性杂草种群形成的原因有两种观点：一种认为杂草长期与除草剂接触，而使除草剂作用部位的编码基因发生突变或者基因表达发生改变，结果导致杂草对除草剂的解毒能力提高或者出现对除草剂具有耐药性或抗药性的种群。总的来说，杂草抗性的形成，基本上是一个抗性基因被选择的过程，抗药性基因的遗传是杂草产生抗药性的基础。之前的报道认为，抗药性的产生不仅是由于植物改变了对除草剂的吸收与转运或植物加速了除草剂的代谢，且主要是因为除草剂的作用部位发生了改变。目前，尚缺少可以有效利用的ACCase突变型基因，限制了抗除草剂植物材料的创制和该类除草剂的高效利用。

发明内容

技术问题：禾本科植物对ACCase抑制剂类除草剂高度敏感，本发明所要解决的技术问题是提供一种可以赋予植物抗除草剂抗性的突变型基因和突变型蛋白。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗(耐)乙酰辅酶A羧化酶类除草剂抗性的小麦ACCase突变型基因和突变型蛋白。

技术方案：通过定点突变的方法得到ACCase基因突变型，利用分子生物学的方法克隆该ACCase突变型基因，然后将该基因用遗传转化的方法导入对除草剂敏感的受体植物基因组，表达ACCase突变型蛋白，使受体植物具有对ACCase抑制剂类除草剂的抗性。所述ACCase突变型基因编码蛋白的氨基酸序列存在如下突变：其对应于野生型小麦ACCase的氨基酸序列的第1708位发生突变、第1768位发生突变、第1769位发生突变、第1992位发生突变、第2015位发生突变、第2029位发生突变、第2066位发生突变、第2084位发生突变。野生型小麦ACCase的氨基酸序列的第1708位原为精氨酸，突变后可为除精氨酸之外的任意氨基酸；第1768位原为天冬酰胺，突变后可为除天冬酰胺之外的任意氨基酸；第1769位原为异亮氨酸，突变后可为除异亮氨酸之外的任意氨基酸；第1992位原为丙氨酸，突变后可为除丙氨酸之外的任意氨基酸；第2015位原为色氨酸，突变后可为除色氨酸之外的任意氨基酸；第2029位原为异亮氨酸，突变后可为除异亮氨酸之外的任意氨基酸；第2066位原为天冬氨酸，突变后可为除天冬氨酸之外的任意氨基酸；第2084位原为甘氨酸，突变后可为除甘氨酸之外的任意氨基酸。

所述小麦ACCase突变型基因表达的蛋白，包括：

(a)其氨基酸序列选自由SEQ ID NO.2、SEQ ID NO.4、SEQ ID NO.6、SEQ ID NO.8、SEQ ID NO.10、SEQ ID NO.12、SEQ ID NO.14、SEQ ID NO.16组成的组中；或

(b)在(a)中的氨基酸序列经过取代和/或缺失和/或添加一个或多个氨基酸，且具有乙酰辅酶A羧化酶活性的由(a)衍生的蛋白质。

本发明还提供一种核酸或基因，其编码所述的蛋白。

本发明所述的核酸或基因，包括：

(i)编码所述的蛋白的核酸或基因；或

(ii)在严格条件下与(i)限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或

(iii)其核苷酸序列选自由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:9、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:15组成的组中。

所述严格条件为：在0.1×SSC、0.1％SDS的溶液中，65℃洗膜15分钟的高度严谨的杂交条件。

本发明的ACCase突变型基因发生突变的核苷酸包括但不仅限于以下几种突变，在以下位点突变成其他核苷酸的情况也在本发明的保护范围之内，例如：A突变为T、C、G；或C突变为T、A、G；或T突变为A、C、G；或G突变为T、C、A等等均在本发明的保护范围之内。

进一步地，根据本发明的小麦ACCase突变型基因，所述小麦ACCase基因存在以下一种或多种突变：只在小麦ACCase基因的第5122位核苷酸发生突变、只在小麦ACCase基因的第5303位核苷酸发生突变、只在小麦ACCase基因的第5309位核苷酸发生突变、只在小麦ACCase基因的第5975位核苷酸发生突变、只在小麦ACCase基因的第6045位核苷酸发生突变、只在小麦ACCase基因的第6085位核苷酸发生突变、只在小麦ACCase基因的第6197位核苷酸发生突变、只在小麦ACCase基因的第6250位核苷酸发生突变、或多个位点同时发生突变，例如第5122位核苷酸和第5303位核苷酸同时发生突变，第5122位核苷酸、第5303位核苷酸和第5309位核苷酸同时发生突变、第5122位核苷酸、第5303位核苷酸、第5309位核苷酸和第5975位核苷酸同时发生突变等。

作为优选，根据本发明的小麦ACCase突变型基因，所述小麦ACCase基因存在以下一种或多种突变：只在小麦ACCase基因的第5122位核苷酸由C突变为T、只在小麦ACCase基因的第5303位核苷酸由A突变为G、在小麦ACCase基因的第5309位核苷酸由A突变为T、在小麦ACCase基因的第5975位核苷酸由C突变为T、在小麦ACCase基因的第6045位核苷酸由G突变为T、在小麦ACCase基因的第6085位核苷酸由T突变为A、在小麦ACCase基因的第6197位核苷酸由A突变为G、在小麦ACCase基因的第6250位核苷酸由G突变为A，或多个位点同时发生突变。

本发明还提供一种表达盒、重组载体或细胞，其含有所述的ACCase突变型基因。

所述除草剂是禾草灵和盖草能中的一种或多种。

本发明还提供获得具有除草剂抗性的植物的方法，包括如下步骤：

1)使植物包含所述核酸或基因；或

2)使植物表达所述小麦ACCase突变型蛋白。

进一步地，所述方法包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。

本发明还提供一种鉴定植物的方法，其中所述植物是包含所述的核酸或基因的植物、表达所述的蛋白的植物或由所述的方法获得的植物，包括以下步骤：

1)测定所述植物是否包含所述的核酸或基因；或，

2)测定所述植物是否表达所述的蛋白。

本发明还提供一种除草剂的防护剂，该防护剂包括所述的小麦ACCase突变型蛋白。

本发明还提供一种控制杂草的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或所述的表达盒、重组载体或细胞，所述除草剂为禾草灵和盖草能中的一种或多种。

本发明还提供一种用于保护植物免受由除草剂引起的损伤的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或将所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为禾草灵和盖草能中的一种或多种。

有益效果：本发明公开的来源于小麦的ACCase突变型基因及其应用方法可以赋予除草剂敏感植物对除草剂的抗性，籍此可以培育抗除草剂植物，从而实现农作物大田高效化学除草。所述小麦ACCase突变型基因还可以通过杂交、回交等传统育种方法向普通小麦品种转移，培育抗除草剂小麦新品种。

附图说明

图1示出了含有ACCase突变位点的转基因小麦抗除草剂的示意图。

图2示出了PCR扩增含有小麦ACCase突变位点的ACCase基因全长结果图；第1泳道为Marker；Marker分子量从上到下依次为15kb、8kb、5kb、3kb、2kb、1.5kb、1kb、750bp、500bp，第2泳道为野生型徐麦33号的DNA，第3泳道为含有抗除草剂突变位点ACCase-1708的DNA，第4泳道为含有抗除草剂突变位点ACCase-1768的DNA，第5泳道为含有抗除草剂突变位点ACCase-1769的DNA，第6泳道为含有抗除草剂突变位点ACCase-1992的DNA，第7泳道为含有抗除草剂突变位点ACCase-2015的DNA，第8泳道为含有抗除草剂突变位点ACCase-2029的DNA，第9泳道为含有抗除草剂突变位点ACCase-2066的DNA，第10泳道为含有抗除草剂突变位点ACCase-2084的DNA。

图3示出了突变型ACCase基因转基因小麦PCR检测图；总共有8个泳道，第1泳道为Marker；Marker分子量从上到下依次为15kb、8kb、5kb、3kb、2kb、1.5kb、1kb、750bp、500bp；第2泳道为非转基因野生型DNA，作为阴性对照，第3泳道为质粒DNA，作为阳性对照，第4泳道为转化含有ACCase-1708抗除草剂突变体ACCase基因的转基因植株DNA，第5泳道为转化含有ACCase-1768抗除草剂突变体ACCase基因的转基因植株DNA，第6泳道为转化含有ACCase-1769抗除草剂突变体ACCase基因的转基因植株DNA，第7泳道为转化含有ACCase-1992抗除草剂突变体ACCase基因的转基因植株DNA，第8泳道为转化含有ACCase-2015抗除草剂突变体ACCase基因的转基因植株DNA，第9泳道为转化含有ACCase-2029抗除草剂突变体ACCase基因的转基因植株DNA，第10泳道为转化含有ACCase-2066抗除草剂突变体ACCase基因的转基因植株DNA，第11泳道为转化含有ACCase-2084抗除草剂突变体ACCase基因的转基因植株DNA。

具体实施方式

下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。

实施例1：小麦ACCase基因突变位点的确定

我们将小麦的ACCase序列(Genbank XM_015783727.2)作为参考序列，与小麦基因组进行同源比对，发现与小麦基因组的ACCase基因(AF029895.1)的同源性最高。根据专利(US15715547、CN201811176178.6)中提到的水稻ACCase可以抗除草剂的氨基酸突变位点，分别是1731(精氨酸突变为半胱氨酸)、1791(天冬酰胺突变为丝氨酸)、1792(异亮氨酸变为亮氨酸)、2015(丙氨酸变为缬氨酸)、2038(色氨酸变为半胱氨酸)、2052(异亮氨酸变为天冬酰胺)、2089(天冬氨酸变为甘氨酸)、2107(甘氨酸变为丝氨酸)作为参考，通过同源比对的方法，确定在小麦中ACCase基因突变的氨基酸位点分别为1708(精氨酸突变为半胱氨酸)、1768(天冬酰胺突变为丝氨酸)、1769(异亮氨酸变为亮氨酸)、1992(丙氨酸变为缬氨酸)、2015(色氨酸变为半胱氨酸)、2029(异亮氨酸变为天冬酰胺)、2066(天冬氨酸变为甘氨酸)、2084(甘氨酸变为丝氨酸)。根据氨基酸密码子对照表，确定对应核苷酸位点分别为5122(C变为T)、5303(A变为G)、5309(A变为T)、5975(C变为T)、6045(G变为T)、6085(T变为A)、6197(A变为G)、6250(G变为A)。

实施例2：小麦徐麦33号野生型ACCase全长基因的获得

选取徐麦33号野生型植株的叶片，提取基因组DNA，根据NCBI中小麦ACCase基因(NCBI Genbank：AF029895)设计引物进行扩增，采用KOD DNA polymerase聚合酶(购自Toyobo公司)扩增ACCase基因，其反应体系如下：

PCR扩增反应程序采用两步法，退火和延伸合为一起，采用68度。

程序如下：预变性：94℃3min；35个循环：变性94℃10sec；延伸68℃10min；保温：72℃10min。

取2μl PCR产物经1％琼脂糖凝胶电泳检测，发现有预期大小的片段后(图2)，剩余的PCR产物经PCR清洁试剂盒(购自Axygen公司)清洁回收后，克隆至pMD19-T载体(购自Takara公司)，然后转化大肠杆菌。每个转化随机挑取12个大肠杆菌单克隆进行PCR检测，取PCR结果呈阳性的6个单克隆，送南京一道生物科技有限公司测序，获得突变ACCase基因序列，该基因片段长度为13127bp，通过去除内含子后获得基因全长6936bp，徐麦33号野生型ACCase序列全长基因片段的测序结果如SEQ ID No:17所示。

实施例3：小麦ACCase基因突变位点基因序列的获得

利用点突变试剂盒扩增小麦ACCase基因的突变位点序列。首先，利用引物ACC-CF和ACC-CR，以徐麦33号cDNA为模板，克隆ACCase全长cDNA，连入TK007载体(南京擎科生物科技有限公司)，转化大肠杆菌TOP10菌株感受态细胞，挑选阳性转化子测序验证，获得正确单克隆，质粒命名为TK007-ACCase；第二，利用突变引物ACC-MutF和ACC-MutR，以TK007-ACCase为模版，进行PCR扩增，扩增产物限制性内切酶DpnI消化后，转入大肠杆菌DMT菌株感受态细胞，挑选阳性转化子测序验证，获得ACCase目标位点突变的质粒。

利用突变引物ACC1708-MutF和ACC1708-MutR扩增ACCase-1708突变位点，其核苷酸序列的第5122位由C突变为T，导致其编码的氨基酸序列的第1708位由精氨酸变为半胱氨酸，其核苷酸序列如SEQ ID No:1所示，其编码的氨基酸序列如SEQ ID No:2所示；

利用突变引物ACC1768-MutF和ACC1768-MutR扩增ACCase-1768突变位点，其核苷酸序列的第5303位由A突变为G，导致其编码的氨基酸序列的第1768位由天冬酰胺变为丝氨酸，其核苷酸序列如SEQ ID No:3所示，其编码的氨基酸序列如SEQ ID No:4所示；

利用突变引物ACC1769-MutF和ACC1769-MutR扩增ACCase-1769突变位点，其核苷酸序列的第5309位由A突变为T，导致其编码的氨基酸序列的第1769位由异亮氨酸变为亮氨酸，其核苷酸序列如SEQ ID No:5所示，其编码的氨基酸序列如SEQ ID No:6所示；

利用突变引物ACC1992-MutF和ACC1992-MutR扩增ACCase-1992突变位点，其核苷酸序列的第5975位由C突变为T，导致其编码的氨基酸序列的第1992位由丙氨酸变为缬氨酸，其核苷酸序列如SEQ ID No:7所示，其编码的氨基酸序列如SEQ ID No:8所示；

利用突变引物ACC2015-MutF和ACC2015-MutR扩增ACCase-2015突变位点，其核苷酸序列第6045位由G突变为T，导致其编码的氨基酸序列的第2010位由色氨酸变为半胱氨酸，其核苷酸序列如SEQ ID No:9所示，其编码的氨基酸序列如SEQ ID No:10所示。

利用突变引物ACC2029-MutF和ACC2029-MutR扩增ACCase-2029突变位点，其核苷酸序列的第6085位由T突变为A，导致其编码的氨基酸序列的第2029位由异亮氨酸变为天冬酰胺，其核苷酸序列如SEQ ID No:11所示，其编码的氨基酸序列如SEQ ID No:12所示。

利用突变引物ACC2066-MutF和ACC2066-MutR扩增ACCase-2066突变位点，其核苷酸序列的第6197位由A突变为G，导致其编码的氨基酸序列第2066位由天冬氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:13所示，其编码的氨基酸序列如SEQ ID No:14所示。

利用突变引物ACC2084-MutF和ACC2084-MutR扩增ACCase2084突变位点，其核苷酸序列第6250位由G突变为A，导致其编码的氨基酸序列第2084位由甘氨酸变为丙氨酸，其核苷酸序列如SEQ ID No:15所示，其编码的氨基酸序列如SEQ ID No:16所示。

实施例4 ACCase转基因小麦的获得及其抗除草剂验证

通过Infuison反应，将突变的ACCase片段连入含有花椰菜花叶病毒35S启动子和35S终止子的植物表达载体中，分别转化野生型小麦。获得纯合并稳定表达含有ACCase突变位点的转基因小麦，并对转基因小麦检测除草剂的耐受性。

将转基因小麦和野生型小麦分别播种，待小麦长至三叶期时，分别喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用1.5mL喹禾灵/L水(2倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为施用1.5mL吡氟氯禾灵/L水(1倍推荐使用浓度))、烯草酮(喷施浓度为施用1.5mL烯草酮/L水(1倍推荐使用浓度))、吡氟禾草灵(喷施浓度为施用1.5mL吡氟禾草灵/L水(1倍推荐使用浓度))，喷施一个月后调查枯死情况，发现野生型对照在喷施和以上同样浓度的乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵、烯草酮、吡氟禾草灵后，几乎停止生长，新长出来的叶片完全枯黄，并逐渐枯死。而含有ACCase突变位点的转基因小麦则全部对乙酰辅酶A羧化酶抑制剂类除草剂表现出抗的表型，其正常的生长发育几乎没有受到影响，最终正常拔节抽穗。表1中显示本发明转基因小麦对ACCase抑制性除草剂的抗性比野生型要高，伤害百分比要低60％以上。这表明我们获得的携带有ACCase突变位点的转基因小麦确实能够稳定遗传的获得抗乙酰辅酶A羧化酶抑制剂类除草剂的抗性。

表1乙酰辅酶A羧化酶类除草剂对野生型和转基因小麦的伤害比率

尽管本发明的具体实施方式已经得到详细描述，本领域技术人员将会理解，根据已经公开的所有教导，可以对那些细节进行各种修改和替换，这些均在本发明的保护范围内。本发明的全部范围由所附专利要求极其任何等同物给出。

序列表

<110> 江苏省农业科学院

<120> 一种小麦ACCase突变型基因及其在植物抗除草剂中的应用

<160> 36

<170> SIPOSequenceListing 1.0

<210> 1

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-1708突变核苷酸序列)

<400> 1

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ctgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 2

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-1708突变氨基酸序列)

<400> 2

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Cys Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 3

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-1768突变核苷酸序列)

<400> 3

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gagcatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 4

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-1768突变氨基酸序列)

<400> 4

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Ser Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 5

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-1769突变核苷酸序列)

<400> 5

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatactt ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 6

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-1769突变氨基酸序列)

<400> 6

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Leu His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 7

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-1992突变核苷酸序列)

<400> 7

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagttacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 8

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-1992突变氨基酸序列)

<400> 8

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Val Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 9

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-2015突变核苷酸序列)

<400> 9

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actgtagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 10

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-2015突变氨基酸序列)

<400> 10

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Cys Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 11

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-2029突变核苷酸序列)

<400> 11

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaaacctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 12

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-2029突变氨基酸序列)

<400> 12

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Asn Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 13

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-2066突变核苷酸序列)

<400> 13

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattggtag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 14

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-2066突变氨基酸序列)

<400> 14

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Gly Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 15

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase-2084突变核苷酸序列)

<400> 15

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaaga gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 16

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase-2084突变氨基酸序列)

<400> 16

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Ser Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 17

<211> 6936

<212> DNA

<213> 徐麦33号(Triticum aestivum L.ACCase野生型核苷酸序列)

<400> 17

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 18

<211> 2311

<212> PRT

<213> 徐麦33号(Triticum aestivum L.ACCase野生型氨基酸序列)

<400> 18

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 19

<211> 25

<212> DNA

<213> ACCase基因野生型序列ACC-CF引物(Artificial Sequence)

<400> 19

atgacatcca cacatgtggc gacat 25

<210> 20

<211> 25

<212> DNA

<213> ACCase基因野生型序列ACC-CR引物(Artificial Sequence)

<400> 20

tcaaccaagg accttcctga tttct 25

<210> 21

<211> 20

<212> DNA

<213> ACCase-1708突变位点ACC1708-MutF引物(Artificial Sequence)

<400> 21

ttgcttctgt gttggatggt 20

<210> 22

<211> 20

<212> DNA

<213> ACCase-1708突变位点ACC1708-MutR引物(Artificial Sequence)

<400> 22

ccaacacaga agcaagattt 20

<210> 23

<211> 20

<212> DNA

<213> ACCase-1768突变位点ACC1768-MutF引物(Artificial Sequence)

<400> 23

gtggagagca tacatggaag 20

<210> 24

<211> 20

<212> DNA

<213> ACCase-1768突变位点ACC1768-MutR引物(Artificial Sequence)

<400> 24

atgtatgctc tccacaccta 20

<210> 25

<211> 20

<212> DNA

<213> ACCase-1769突变位点ACC1769-MutF引物(Artificial Sequence)

<400> 25

aacatacttg gaagtgctgc 20

<210> 26

<211> 20

<212> DNA

<213> ACCase-1769突变位点ACC1769-MutR引物(Artificial Sequence)

<400> 26

acttccaagt atgttctcca 20

<210> 27

<211> 20

<212> DNA

<213> ACCase-1992突变位点ACC1992-MutF引物(Artificial Sequence)

<400> 27

gattcagtta ctaagacagc 20

<210> 28

<211> 20

<212> DNA

<213> ACCase-1992突变位点ACC1992-MutR引物(Artificial Sequence)

<400> 28

cttagtaact gaatctggaa 20

<210> 29

<211> 20

<212> DNA

<213> ACCase-2015突变位点ACC1992-MutF引物(Artificial Sequence)

<400> 29

ctaactgtag aggcttctct 20

<210> 30

<211> 20

<212> DNA

<213> ACCase-2015突变位点ACC2015-MutR引物(Artificial Sequence)

<400> 30

agcctctaca gttagcaagg 20

<210> 31

<211> 20

<212> DNA

<213> ACCase-2029突变位点ACC2029-MutF引物(Artificial Sequence)

<400> 31

tgaaggaaac cttcaggctg 20

<210> 32

<211> 20

<212> DNA

<213> ACCase-2029突变位点ACC2029-MutR引物(Artificial Sequence)

<400> 32

tgaaggattc cttcaaaaag 20

<210> 33

<211> 20

<212> DNA

<213> ACCase-2066突变位点ACC2066-MutF引物(Artificial Sequence)

<400> 33

gtgattggta gcaagataaa 20

<210> 34

<211> 20

<212> DNA

<213> ACCase-2066突变位点ACC2066-MutR引物(Artificial Sequence)

<400> 34

cttgctacca atcacgaccc 20

<210> 35

<211> 20

<212> DNA

<213> ACCase-2084突变位点ACC2084-MutF引物(Artificial Sequence)

<400> 35

tgcaaagagc aatgttctcg 20

<210> 36

<211> 20

<212> DNA

<213> ACCase-2084突变位点ACC2084-MutR引物(Artificial Sequence)

<400> 36

acattgctct ttgcagtcct 20

Claims

1.一种小麦ACCase突变型蛋白，其特征在于，所述蛋白的氨基酸对应于野生型小麦ACCase的氨基酸序列的第2084位由甘氨酸突变为丙氨酸，其氨基酸序列如SEQ ID NO.16所示。

2.一种ACCase突变型基因，其特征在于：其编码根据权利要求1所述的蛋白。

3.根据权利要求2所述的ACCase突变型基因，其特征在于：其核苷酸序列如SEQ ID NO：15所示。

4.根据权利要求2或3所述的ACCase突变型基因，其特征在于，将所述ACCase突变型基因导入小麦，可以使受体小麦具有对除草剂的抗性，所述除草剂选自喹禾灵、烯草酮和吡氟禾草灵。

5.根据权利要求4所述的ACCase突变型基因，其特征在于，将所述ACCase突变型基因导入受体小麦的方法是转基因、杂交或回交。

6.根据权利要求5所述的ACCase突变型基因，其特征在于，所述转基因方法，包括如下步骤：

1）构建含有ACCase突变型基因的表达载体；

2）将上述表达载体通过遗传转化小麦，使小麦包含根据权利要求2或3所述的ACCase突变型基因；

3）使小麦表达根据权利要求1所述的小麦ACCase突变型蛋白。

7.一种含有根据权利要求2或3所述的ACCase突变型基因的表达载体。

8.一种含有根据权利要求2或3所述的ACCase突变型基因的表达载体的宿主细胞。