CN108476853A - A method of control North America Acer palmatum ' Atropurpureum' leaf color change - Google Patents
A method of control North America Acer palmatum ' Atropurpureum' leaf color change Download PDFInfo
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- CN108476853A CN108476853A CN201810323871.5A CN201810323871A CN108476853A CN 108476853 A CN108476853 A CN 108476853A CN 201810323871 A CN201810323871 A CN 201810323871A CN 108476853 A CN108476853 A CN 108476853A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The present invention relates to garden afforestation technology fields, a kind of more particularly to method of control North America Acer palmatum ' Atropurpureum' leaf color change, the method is to carry out band processing in the lower part of the North America minimum branch of Acer palmatum ' Atropurpureum' tree trunk, and release band after 2 years, band processing is first to be affixed to trunk on one side by what coating sustained release cloth had a drug, it is fixed again with bandage, drug solution is coated on the coating sustained release cloth, contains agar, sucrose, fructose, plant growth regulator in the drug solution.Without using spraying equipment when the present invention operates, directly carry out the processing of simple band and can shift to an earlier date North America Acer palmatum ' Atropurpureum' leaf color to redden, and do not influence North America Acer palmatum ' Atropurpureum' physiological mechanism and normal growth, it is easy to operate, equipment cost is saved, avoiding external source simultaneously and spray causes Foliage Absorption uneven, influences the unconspicuous situation of leaf color change.
Description
Technical field
The present invention relates to garden afforestation technology field more particularly to a kind of methods of control North America Acer palmatum ' Atropurpureum' leaf color change.
Background technology
With the rapid development of China's economic level, people’s lives level is continuously improved, and Process of Urbanization Construction paces are continuous
Accelerate, more and more people begin to focus on urban construction, and traditional afforestation has been unable to meet the development of people's spiritual culture
Demand, afforestation industry have been acknowledged as " eternal rising industry " in world wide, and excellent color leafed plants are that promotion is green
Change the important foundation of quality, so-called color leafed plants refer in all or part of a certain stage of the entire season of growth or the season of growth
Blade the plant of non-green is more steadily presented.But it is in great shortage to show excellent color tree species in China market at present, especially
It is large-scale color leaf arbor, this causes considerable hurdle to the promotion of gardens quality.
North America Acer palmatum ' Atropurpureum' (Acer rubrum L.) spring opens safflower, knot samara, and summer tree crown is dense, shading cools down, autumn
Leaf color is scarlet, leaf grace, and winter blade withers and falls, ventilation and penetrating light, not only cold-resistant, drought-enduring, moisture-proof, moreover it is possible to absorb atmosphere pollution
Object is very useful color leaf Landscape Tree Species.However, with the reduction of latitude, color changeable effect is in reducing tendency, or discoloration is very
Late, or the not equal blade that changes colour just withers and falls, and ornamental plantation effect is not achieved.As can by simple artificial regulatory measure, promote north
U.S.'s improvement Acer palmatum ' Atropurpureum' changes colour ahead of time, extends its red autumnal leaves time, and the application of Acer palmatum ' Atropurpureum' is improved to North America will generate tremendous influence, while
Considerable economic benefit can be obtained.For many years, the method for artificial regulatory leaf color focuses mostly on sprays in external source, external source injection
It is uneven by spraying the liquid trickled down on blade although having a certain impact to Acer palmatum ' Atropurpureum' discoloration, it be easy to cause correlation
Material absorbing is uneven, preferable regulating effect is not achieved, and hand spray is needed by spraying equipment, equipment cost is high, behaviour
Make complex.
Invention content
In view of this, the object of the present invention is to provide a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change, when operation, is not necessarily to
Using spraying equipment, simple band processing is directly carried out, it is easy to operate, equipment cost is saved, while avoiding external source
It sprays and causes Foliage Absorption uneven, influence the unconspicuous situation of leaf color change.
The present invention solves above-mentioned technical problem by following technological means：
A method of control North America Acer palmatum ' Atropurpureum' leaf color change, the method is in the lower part of the North America minimum branch of Acer palmatum ' Atropurpureum' tree trunk
Band processing is carried out, and releases band after 2 years, the band processing is the face paste for first having coating sustained release cloth drug
It is fixed to trunk, then with bandage.
Further, the width of the coating sustained release cloth and bandage is 2cm.
Further, it is coated with drug solution on the coating sustained release cloth, contains agar, sucrose, fruit in the drug solution
Sugar, plant growth regulator.
Further, 10g/L agar, 35g/L sucrose, 10g/L fructose, 15mg/L plant growths are contained in the drug solution
Conditioning agent.
Further, the preparation method of the drug solution is as follows：It weighs sucrose and fructose is dissolved in distilled water, stirring is extremely
It is completely dissolved, after being heated to 85~95 DEG C, agar is added and stirs to being completely dissolved, is then cooled to 50~60 DEG C, plant is added
Growth stimulator stirs and evenly mixs, and obtains drug solution.
Further, the preparation method of the coating sustained release cloth is as follows：The width of sustained release cloth is cut to 2cm, is being sustained
The one side of cloth brushes drug solution, and it is solidifying to repeat drug solution brushing for the drug solution solidification being then cooled on sustained release cloth
Gu five times, being finally dried in vacuo to sustained release cloth, coating sustained release cloth is obtained.
The sustained release cloth of the present invention is to infiltrate common cloth in sustained-release liquid, after freeze-dried, on common cloth
Porous composite film is formed, contains chitosan, TiO 2 porous microspheres and Arabic gum in sustained-release liquid therein.Gathered by containing shell
Sugar, TiO 2 porous microspheres and Arabic gum sustained-release liquid form porous composite film on common cloth, composite membrane it is porous
Structure and TiO 2 porous microspheres, which to be sustained on cloth, has larger specific surface area, and it is molten to drug to be conducive to sustained release cloth
The absorption of active ingredient in liquid is particularly advantageous for load of the plant growth regulator on sustained release cloth, and in sequoia sempervirens
During band, slow releasing function is played to the active ingredient in drug solution, it can continuous and effective release.
The present invention handles leaf color change for regulating and controlling North America Acer palmatum ' Atropurpureum' by artificial band, and when operation sets without using injection
It is standby, simple band processing is directly carried out, it is easy to operate, equipment cost is saved, while avoiding external source and spray and causing leaf
Face absorption is uneven, influences the unconspicuous situation of leaf color change.The methods and results detection of the present invention shows that band processing can promote
Into the synthesis of Photosynthetic Pigment and anthocyanin, and then Acer palmatum ' Atropurpureum' leaf color in North America reddens in advance, and the elements such as P, Zn element are not
Apparent variation occurs, thus does not interfere with North America Acer palmatum ' Atropurpureum' physiological mechanism and normal growth.
Description of the drawings
Fig. 1 is a kind of band processing schematic diagram of the method for control North America Acer palmatum ' Atropurpureum' leaf color change of the present invention；
Fig. 2 be the present invention take a schematic diagram；
Fig. 3 is that the leaf color parameter for the North America Acer palmatum ' Atropurpureum' Different sites of leaf that the present invention tests contains spirogram；
Fig. 4 is the Contents of Photosynthetic Pigments figure for the North America Acer palmatum ' Atropurpureum' Different sites of leaf that the present invention tests；
Fig. 5 is the Anthocyanin content figure for the North America Acer palmatum ' Atropurpureum' Different sites of leaf that the present invention tests.
Specific implementation mode
Below with reference to the drawings and specific embodiments, the present invention is described in detail：
A kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change of the present invention is to weigh 35g sucrose, the dissolving of 10g fructose first
It in 1L distilled waters, stirs to being completely dissolved, after being heated to 85~95 DEG C, 10g agar is added and stirs to being completely dissolved, it is then cold
But to 50~60 DEG C, 15mg plant growth substances is added and stir and evenly mix, obtain drug solution；The width of sustained release cloth is cut to 2cm,
Drug solution is brushed in the one side of sustained release cloth, brushing will extremely be sustained cloth complete wetting, be then cooled on sustained release cloth
Drug solution solidifies, and repeats drug solution and brushes solidification five times, is finally dried in vacuo to sustained release cloth, obtains coating sustained release
Cloth；Finally, it is affixed to trunk on one side by what coating sustained release cloth had a drug, then is fixed with bandage and carries out band processing, Yu Liangnian
After release band.
The sustained release cloth of the present embodiment is by the infiltration of common cloth in sustained-release liquid, after freeze-dried, in common cloth
Upper formation porous composite film contains chitosan, TiO 2 porous microspheres and Arabic gum in sustained-release liquid therein.The sustained-release liquid
Be formulated as follows：
TiO 2 porous microspheres are prepared first, and 7g butyl titanate stirring and dissolvings are added in every 10mL, are then added
2g triblock copolymers continue to obtain homogeneous phase solution after stirring 1h, and it is small that obtained homogeneous phase solution at 65 DEG C is heated to reflux half
When, it is cooled to room temperature to obtain TiO 2 sol, anode is then made using copper wire, anode and the moon is arranged as cathode in aluminium foil
The distance between pole is 20cm, and EFI is carried out under 12kV voltages, titanium dioxide microballoon sphere is finally obtained on aluminium foil, by titanium dioxide
Titanium microballoon is placed in 600 DEG C of Muffle furnace roasting 2h, and furnace cooling is taken out, and grinding obtains TiO 2 porous microspheres；Secondly, it prepares
Buffer solutions weigh chitosan stirring and dissolving in the acetum that mass concentration is 1.5%, and it is 0.8% to obtain mass concentration
Chitosan solution, be positioned over spare in 60 DEG C of thermostat water bath, in addition weigh 1g Arabic gums and 0.2g titanium dioxide is more
Hole microballoon, is put into mortar and is ground mixing 10min, and 2mL distilled water is then added and continues to be ground to form colostrum, adds
20mL distilled water, obtains mixed emulsion, and mixed emulsion is stirred and is added in 50mL chitosan solutions, and adjusts pH=4.0, then
It adds 50mL60 DEG C of distilled water to stir and evenly mix, obtains sustained-release liquid, be stored in spare in 60 DEG C of thermostat water bath.
Common cloth is completely submerged in sustained-release liquid, is mixed up per 10min, after impregnating 30min, take out cloth by its
It is placed in freeze drier and is dried, take out and brush sustained-release liquid on cloth surface, then be freeze-dried, repeat to brush freezing step
Altogether three times, the cloth after last freeze-drying in the sodium hydroxide solution that mass concentration is 0.2% is impregnated into 5min, taken out cold
Be lyophilized it is dry, obtain sustained release cloth.
Specific embodiment mode and effect detection are as follows：
Weigh 35g sucrose, 10g fructose is dissolved in 1L distilled waters, stir to being completely dissolved, after being heated to 85~95 DEG C,
10g agar is added to stir to being completely dissolved, is then cooled to 50~60 DEG C, 15mg plant growth substances are added and stir and evenly mix, obtain
Drug solution.The width of sustained release cloth is cut to 2cm, drug solution is brushed in the one side of sustained release cloth, is then cooled to sustained release
Drug solution solidification on cloth, repeats drug solution and brushes solidification five times, is finally dried in vacuo, obtains to sustained release cloth
Coating is sustained cloth.
Experiment material used in the present embodiment is transplanted in the North America Acer palmatum ' Atropurpureum' cultivation in the school district experimental plot of Southwestern University south
Kind " wine is red ", all experimental plants carry out the Routine Managements such as unifying fertilising, water, hoe up weeds.It on March 1st, 2015 chooses and divides
Fulcrum is relatively low, have and only there are two the plant of the consistent branch of growing way as test tree, wherein the middle part progress of a branch
Band processing is first affixed to trunk by what coating sustained release cloth had a drug, then is fixed with bandage on one side.By the branch of band
It is denoted as SD, i.e. band group, by the CK that is denoted as of not band, i.e. control group.And the lower part of band group branch is denoted as S1, top note
For S2；The lower part of control group is denoted as C1, top is denoted as C2, and specific band label situation is as shown in Figure 1.Autumn in 2016 is seen
Observe control group and test group leaf color difference clearly, and the band position of band branch is obviously thinner than top and the bottom trunk, normally
Growth is serious impacted, band processing is released in April, 2017, and carry out the measurement of indices, specific as follows：
It since each tree possesses exclusive physiological mechanism, is interfered in addition with band, causes each tree, each handles branch
The blade discoloration phase it is inconsistent, therefore showed using the leaf color of the experiment branch S1 to redden at first as standard for manual sampling, i.e., at every
The picking leaves when blade of S1, tree is in best red autumnal leaves viewing period, sampling time are 8 days-November 22 October in 2017.Sampling
When, it is each to handle branch helical form uniform sampling about 20 from top to bottom, experiment is taken back rapidly in the freshness protection package loaded on corresponding label
Room.First blade is cleaned, is dried, pictorial information is then acquired, then by the water-removing of a part of blade, drying, for mineral element
It measures, another part blade removes arteries and veins, shreds, measurement of the mixing for physical signs.
Acquire the pictorial information of blade：It is right using digital camera (Nikon D7000, Nikon Corp, Tokyo)
The North America Acer palmatum ' Atropurpureum' fresh leaf clean, dried is taken pictures, and when taking pictures, is fixed camera position indoors using camera frame, camera mould
Formula be adjusted to it is M grades manual, parameter adjustment be aperture F=4.5, shutter 320s, sensitivity iso=500.It is white that one is pasted on white wall
Color A4 paper before blade is temporarily fixed at blank sheet of paper with three white lines, takes pictures only stablize fluorescent lamp as unique light source every time.
Photograph picks up reading blade face in color device Photoshop (CS6, Adobe company, the U.S. add the states Li Fuliya) software
Color parameter, every blade uniformly take 8 color dots in similar position, specifically take color bits to set Fig. 2 such as and note, each processing 5
It repeats.L*, a* and b* value are recorded using Lab space colour model, wherein parameter L* indicates gloss brightness, and the bigger brightness of L* is more
It is high；The value range of parameter a* is [- 128,128], and positive value indicates that color and luster is red, and negative value indicates that color and luster is green；Parameter b*'s takes
Ranging from [- 128,128], positive value indicates yellow degree, negative values blueness degree to value.
The measurement of other physical signs：Anthocyanin content in blade is first extracted with 1% ethanol solution hydrochloride, then with newly
Century ultraviolet specrophotometer (T6, Beijing Puxi General Instrument Co., Ltd, BeiJing, China) measures extracting solution in 600nm
With the absorbance value (Bo Qinnuoke, 1981) of 530nm；The method of the measurement reference X.H. Bo Qinnuoke of photosynthetic pigments, use are ultraviolet
Visible spectrophotometer measures the absorption photometric value at 440nm, 663nm and 645nm；Method of the PPO determinations of activity with reference to Jiang
(Jiang Y.et al., 2000), the method (Lister C E et al., 1996), solvable of the measurement of PAL with reference to lister
Property sugared content using anthrone method (Gao Junfeng, 2006), soluble protein measurement with reference to Xu Jianfeng method (Xu Jianfeng,
2003)；Mineral Elements Content in blade, which with initial reference to the method for Yang Jianhong disappear, boils (Wei Yongsheng etc., 2011), then by inductance
Coupled plasma (ICP-OES, Optima 2100DV types, Perkinelmer Inc. (PE) company, U.S. horse
The states Sa Zhusai) it measures.
Using the one-way analysis of variance (one-way in SPSS 19.0 (IBM Corporation, New York, United States) software
ANOVA) between the indexs such as the leaf color parameter of the North America Acer palmatum ' Atropurpureum' blade of method inspection different disposal, photosynthetic pigments, trace element
The significance of difference, Multiple range test use least significant difference method (LSD).Using Microsoft Excel simply count
According to calculating and drawing processing.As a result as follows：
(1) influence of the band to North America Acer palmatum ' Atropurpureum' blade leaf color parameter
Plant leaf color is typically the external manifestation of inside plants physiological change, measure leaf image lightness parameter L* values,
Leaf color can be showed and be quantized by form and aspect parameter a* values, form and aspect parameter b* values, to infer what internal physiological occurred by numerical value
Variation.In the present embodiment, the leaf color parameter of sample is determined, the results are shown in Figure 3：S2 blade leaf color parameter a* values are significantly higher than
C1、C2、S1(P<0.05) distinct red, is presented, shows that band can significantly affect red presentation；A* values between C1, C2
Difference not significantly (P>0.05), but the a* values of S2 are significantly higher than S1 (P<0.05), differential magnitude 89.92%, and the a* of S1
Value is substantially less than C1 and C2 (P<0.05), show that band causes the substance for making blade that red be presented and shifts, also or be
The matter transportation that certain influence blades redden is hindered, the band top and the bottom substance is caused to redistribute.Upper blade leaf color is joined
Number b* values are less than lower blade, significant difference (P<0.05), the b* values of S2 are substantially less than C2 (P<, but the b* values of S1 and C1 0.05)
Difference significantly (P ＞ 0.05), does not show that North America Acer palmatum ' Atropurpureum' upper blade yellowing degree lower part is apparent, and band also can notable shadow
It rings upper blade yellow to present, with leaf color parameter a* values at opposite variation.The L* values of North America Acer palmatum ' Atropurpureum' upper blade are significantly higher than
Lower part (P<0.05), and the blade lightness L* values of S2 are less than C2, significant difference (P<0.05), but the L* value differences of S1 and C1 are different does not show
It writes (P ＞ 0.05), illustrates that upper blade lower part blade is dark, band can also influence the variation of blade lightness.In conclusion band
The sequence of North America Acer palmatum ' Atropurpureum' blade red degree afterwards is S2>C1>C2>The sequence of S1, yellow degree are S1>C1>C2>S2, blade
The sequence of lightness (from secretly to bright) is S1>C1>C2>S2.
(2) influence of the band to Contents of Photosynthetic Pigments in the Acer palmatum ' Atropurpureum' blade of North America
In the present embodiment, the photosynthetic chromatic number of sample is determined, the results are shown in Figure 4：In blade after the Acer palmatum ' Atropurpureum' band of North America
Though photosynthetic pigments have increase and decrease, variation is not notable, and chlorophyll a (Chl a), chlorophyll b (Chl b) and total leaf are green
Treated that variation tendency is more consistent in band for the content of plain (Chl a+b), and the Contents of Photosynthetic Pigments in upper part blade is equal
It is fewer than lower part；But compared with the control, band branch but shows as higher amount, it may be possible to since band interferes so that North America Acer palmatum ' Atropurpureum' body
Inner chlorophyll accumulates.And the Car contents in the Acer palmatum ' Atropurpureum' upper blade of North America do not change substantially, illustrate band to light and color
The influence of element is smaller.
(3) influence of the band to North America Acer palmatum ' Atropurpureum' blade Anthocyanin content
In the present embodiment, the Anthocyanin content of sample is determined, as shown in Figure 5：Anthocyanin goes out in the Acer palmatum ' Atropurpureum' of North America
Show different variations, the anthocyanin of North America Acer palmatum ' Atropurpureum' upper blade is higher than lower blade, and this species diversity on band branch
More notable (P<0.05), content lower part is high by 69.20%, and this species diversity is not notable in control branch.Band, which is handled, to be made
The Anthocyanin content ratio C2 blades of S2 blades are high by 41.30%, and the difference of S1 and C1 is only 11.12%, shows that band can promote
Into synthesis and accumulation of the anthocyanin above band branch, also or hinders and decompose the substance of anthocyanin and formed, and hinder
Only anthocyanin transports downwards, thus is had no significant effect to the colour generation below branch.
(4) influence of the band to North America Acer palmatum ' Atropurpureum' blade PAL, PPO enzymatic activity and content soluble sugar, soluble protein
In the present embodiment, the phenylalanine lyase of sample, polyphenol oxidase, soluble sugar content, solvable also measured were
Property protein content, the results are shown in Table 1.
Table 1 statistics indicate that：Band is little to the PAL activity influences of North America Acer palmatum ' Atropurpureum', and PAL activities presents are the portion with blade
Position is related, and the PAL activity values of upper blade are slightly larger than lower blade, but the not notable (p of difference>0.05).It is different from PAL, beam
Band influences the PPO activity of North America Acer palmatum ' Atropurpureum' Different sites of leaf, and wherein S2 activity is minimum, and C2 is maximum, the leaf on control branch
Piece PPO activity is in increase variation from the lower to the upper, but is S2 in band branch<S1, this illustrates activity influence of the band to PPO
It is larger.The soluble sugar content of North America Acer palmatum ' Atropurpureum' upper blade will be less than lower part, and S1 outlines are higher than C1, but S2 and C2 contents connect
Closely.It is distributed with soluble sugar content on the contrary, the soluble protein content of North America Acer palmatum ' Atropurpureum' upper blade is slightly above lower blade, and beam
Band branch S1, S2 are below control branch C1, C2, this illustrates that influence of the band to North America Acer palmatum ' Atropurpureum' leaf soluble protein content is little.
Table 1
(5) influence of the band to the Mineral Elements Content in the Acer palmatum ' Atropurpureum' Different sites of leaf of North America
The content of various mineral elements carries out one-way analysis of variance result in North America Acer palmatum ' Atropurpureum' blade after halved tie tape handling
As shown in table 2.The result shows that：North America Acer palmatum ' Atropurpureum' control group and band group show as Ca in the blade of top and the bottom, K element content phase
Consistent variation, the not notable (P of difference>0.05), this illustrates that band does not influence the transport and distribution of Ca, K in the Acer palmatum ' Atropurpureum' of North America.
P element content shows as C2 in control group<C1, but the S2 in band group>S1, and C1>S2>S1>C2, this illustrates that band makes
The distribution mechanism of P element changes in the Acer palmatum ' Atropurpureum' of North America.Zn constituent contents show as C1<C2, but the S1 in band group>S2, and
C2>S1>C1>S2, this illustrates that band affects the distribution of Zn elements from bottom to top in North America Acer palmatum ' Atropurpureum'.Mg, Fe, Mn, Cu in S2
Content be substantially less than S1 (P<0.05), and in control group the difference of top and the bottom is not notable, shows band to North America Acer palmatum ' Atropurpureum'
The transport of these four internal mineral elements influences significantly (P<0.05).The content sequence of control group and S1 blade Mineral Elements is equal
For Ca>Mg>P>K>Fe>Zn>Mn>Sequence in Cu, S2 is Ca>P>Mg>K>Fe>Zn>Mn>Cu, it can be seen that after band Mg and
P element proportion changes, and calcium, the content of magnesium are higher in a great number of elements, trace element in iron, zinc content compared with
It is high.
Table 2
(6) correlativity in North America Acer palmatum ' Atropurpureum' blade between each content of physiologically active substance
To in the Acer palmatum ' Atropurpureum' blade of North America leaf color parameter and physical signs carry out correlation analysis it is as shown in table 3, the results showed that,
Leaf color parameter a* is in extremely notable negative correlativing relation with chlorophyll a, carotenoid, Chlorophyll, with anthocyanin, soluble egg
It is in extremely notable positive correlation in vain, is in notable positive correlation with soluble sugar.
Table 3
The present embodiment with Lab color space models by the leaf color quantity of North America Acer palmatum ' Atropurpureum' turn to leaf color parameter L*, a* and
B* values, and itself and the pigment content in blade are subjected to correlation analysis.Leaf color parameter a* values are blade red green quantifications
Effective expression.Blade discoloration is mainly determined by blade Determination of Chlorophyll and the mutual ratio of Anthocyanin content, is studied in the present embodiment
It was found that the content and a* values of North America Acer palmatum ' Atropurpureum' blade Determination of Chlorophyll and anthocyanin are closely related, and photosynthetic pigments are influenced by band
It is smaller, and difference between control group and band group and not up to notable, band can be embodied all the better makes flower in blade
The content of pigment glycosides increases, and then promotes blade red Coloration Mechanisms of Fancy.At the same time, since there is anthocyanin light to protect energy
Power, thus as Anthocyanin content increases, it is effectively protected the progress of photosynthesis of plant.
The biosynthesis and decomposition of anthocyanin are substantially clear, and wherein PAL enzymes and PPO enzymes are as crucial impact factor
In the presence of.PAL is first key enzyme that reaction is catalyzed in anthocyanin biosynthetic metabolism approach, the not only conjunction of anthocyanin
At precursor is provided, precursor also is provided for the synthesis of other substances, band not yet causes greatly North America Acer palmatum ' Atropurpureum' PAL in the present embodiment
Influence, and PAL and the negatively correlated relationship of anthocyanin.Normally, since PPO has the function of decomposing anthocyanin,
So with the increase of anthocyanin in plant lower part to upper blade, catabolic enzyme increases with also answering, but band group but declines,
This illustrates that band affects the synthesis of PAL enzymes, although also explanation PPO can directly be catalyzed the degradation of anthocyanin, this is not
The main path of anthocyanin degradation.
In the present embodiment, the distribution of North America Acer palmatum ' Atropurpureum' upper blade Mineral Elements is related with blade position, K, Ca, Mg,
The content of Fe, Mn, Cu are below lower part, and band only has Mg, Fe, Mn, Cu a slight influence, and K, Ca content like Zeng Wei by
Influence, searching to the bottom is constrained by inherent mechanism since North America Acer palmatum ' Atropurpureum' mineral element is transported so that root absorption these
Mineral element is difficult in the blade for being transported to band top, causes to be unevenly distributed up and down, and band strengthens transport difficulty, table
It is now that band top content is less than control, and band lower part content is higher than control.Band influences maximum to be P and Zn.According to this reality
It tests result of study and shows that band makes the distribution mechanism of P element change, i.e., band increases P element in upper blade
Accumulation, P element are the indispensable elements that plant maintains biochemical reaction, have its presence in many macromoleculars, and in photosynthetic work
It is acted on important energy transmission is played by the pyrophosphoric acid key of ATP in the process, this illustrates that its increase will ensure photosynthesis
It is normally carried out.And to stablize multi-functional metalloenzyme be the essential trace element of plant to Zn elements as a kind of, content is more
The activity of a variety of enzymes is directly affected less, and the variation of Zn constituent contents is small in this experiment, illustrates that band is influencing leaf color change
While influence to its physiological mechanism it is small.If thinking deeply from the angle of each element content size, the position hair of only P and Mg
Variation is given birth to, i.e., band makes P increase, and Mg is reduced, and the ratio between them is changed.
In conclusion artificial band processing promotes the synthesis of band branch top photosynthetic pigments and anthocyanin, Jin Erti
Preceding North America Acer palmatum ' Atropurpureum' leaf color reddens, and the elements such as P, Zn element do not occur significantly to change, thus does not interfere with North America Acer palmatum ' Atropurpureum'
Physiological mechanism and normal growth.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although with reference to preferred embodiment to this hair
It is bright to be described in detail, it will be understood by those of ordinary skill in the art that, it can modify to technical scheme of the present invention
Or equivalent replacement should all cover the claim in the present invention without departing from the objective and range of technical solution of the present invention
In range.Technology that the present invention is not described in detail, shape, construction part are known technology.
Claims (6)
1. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change, which is characterized in that the method is minimum in North America Acer palmatum ' Atropurpureum' tree trunk
The lower part of branch carries out band processing, and band is released after 2 years, and the band processing is that coating sustained release cloth is first had medicine
Object is affixed to trunk on one side, then is fixed with bandage.
2. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change according to claim 1, which is characterized in that the coating is slow
The width for releasing cloth and bandage is 2cm.
3. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change according to claim 2, which is characterized in that the coating is slow
It releases and is coated with drug solution on cloth, agar, sucrose, fructose, plant growth regulator are contained in the drug solution.
4. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change according to claim 3, which is characterized in that the drug is molten
Contain 10g/L agar, 35g/L sucrose, 10g/L fructose, 15mg/L plant growth regulator in liquid.
5. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change according to claim 4, which is characterized in that the drug is molten
The preparation method of liquid is as follows：It weighs sucrose and fructose is dissolved in distilled water, stir to being completely dissolved, be heated to 85~95 DEG C
Afterwards, agar is added to stir to being completely dissolved, is then cooled to 50~60 DEG C, plant growth substance is added and stirs and evenly mixs, obtains drug
Solution.
6. a kind of method of control North America Acer palmatum ' Atropurpureum' leaf color change according to claim 5, which is characterized in that the coating is slow
The preparation method for releasing cloth is as follows：The width of sustained release cloth is cut to 2cm, drug solution is brushed in the one side of sustained release cloth, with
Drug solution solidification in postcooling to sustained release cloth, repeats drug solution and brushes solidification five times, is finally carried out to sustained release cloth
Vacuum drying obtains coating sustained release cloth.
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| CN113340820A (en) * | 2021-06-11 | 2021-09-03 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113396908A (en) * | 2021-06-17 | 2021-09-17 | 广西壮族自治区林业科学研究院 | Spraying agent for promoting color change of sweetgum leaves |
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| CN113340820A (en) * | 2021-06-11 | 2021-09-03 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113340820B (en) * | 2021-06-11 | 2023-01-24 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113396908A (en) * | 2021-06-17 | 2021-09-17 | 广西壮族自治区林业科学研究院 | Spraying agent for promoting color change of sweetgum leaves |
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| CN108476853B (en) | 2020-07-28 |
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