CN113396908A - Spraying agent for promoting color change of sweetgum leaves - Google Patents

Spraying agent for promoting color change of sweetgum leaves Download PDF

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CN113396908A
CN113396908A CN202110671251.2A CN202110671251A CN113396908A CN 113396908 A CN113396908 A CN 113396908A CN 202110671251 A CN202110671251 A CN 202110671251A CN 113396908 A CN113396908 A CN 113396908A
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leaves
spraying
spraying agent
sweetgum
agent
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CN113396908B (en
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王仁杰
尹国平
蒋燚
刘雄盛
黄荣林
王勇
韦铄星
姜英
肖玉菲
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a spraying agent for promoting the color change of sweetgum leaves, which comprises the raw material components of 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L sucrose. When the spraying agent is used, spraying is carried out in the weather of no rain at 16: 00-18: 00 every 10-15 days from the middle ten days of 10 months to the end ten days of 12 months, the spraying agent is filled into a mist sprayer and then uniformly sprayed on the leaf surfaces of the liquidambar formosana seedlings until the dropping degree of the leaves is reached every time. The spraying agent disclosed by the invention can promote the synthesis of anthocyanin and tannin in liquidambar formosana cells, so that the liquidambar formosana can be promoted to turn red, the color-changing period can be prolonged, and the ornamental value of the liquidambar formosana is improved.

Description

Spraying agent for promoting color change of sweetgum leaves
Technical Field
The invention belongs to the technical field of garden plant maintenance, and relates to a spraying agent for promoting color change of sweetgum leaves.
Background
The Liquidambar formosana Hance is a deciduous tree of Liquidambar (Hamamelidaceae) Liquidambar, is widely distributed and is one of main forest tree species in southern forest areas of China. The liquidambar formosana has strong adaptability and fire resistance, is called as a pioneer tree species in barren mountains, and leaves of the liquidambar formosana gradually change from green to red after autumn, thereby having high ornamental value and being an excellent urban landscape tree species. Due to the reasons of late cooling time, small day and night temperature difference and the like in Guangxi areas, the liquidambar formosana forest stand generally has the problems of poor color changing effect, unstable color changing, color changing without beauty and the like, and the performance of the landscape function is seriously restricted. However, the research on liquidambar formosana at home and abroad mainly focuses on the aspects of chemical components, drug properties, seedling raising technology, community structure, population genetic diversity and the like, and the research on how to promote the liquidambar formosana to change color in advance and prolong the color change period of the liquidambar formosana is only reported.
Disclosure of Invention
The invention provides a spraying agent which can promote the color change of liquidambar formosana and prolong the color change period in order to overcome the defects of the prior art.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a spraying agent for promoting the color change of sweetgum leaves comprises 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L sucrose.
The preparation method of the spraying agent comprises the following steps:
weighing, namely weighing 100mg of abscisic acid, 6mmol of citric acid, 4mmol of salicylic acid, 200mg of cane sugar and the balance of water, wherein the total volume is 1L;
and step two, mixing, namely mixing and dissolving the weighed abscisic acid, citric acid, salicylic acid and cane sugar in water to prepare the spraying agent.
The application method of the spraying agent comprises the following steps: spraying at the ratio of 16: 00-18: 00 every 10-15 days in the middle 10 th to the end 12 th of the month in the rainy-free weather, and uniformly spraying the leaf surfaces of the liquidambar formosana seedlings after the spraying agent is filled into a mist sprayer until the dropping degree of the leaves is reached every time.
Preferably, each time the fertilizer is sprayed, a plastic bag is sleeved on the flowerpot of the sweetgum seedling to prevent soil acidification.
Compared with the prior art, the invention has the following beneficial effects:
1. the liquidambar formosana discoloring agent is not only related to gene and environmental factors, but also plays an important role by exogenous substances, and the spraying agent disclosed by the invention can promote synthesis of anthocyanin and tannin in liquidambar formosana cells, so that liquidambar formosana reddens can be promoted, the discoloring period can be prolonged, and the ornamental value of the liquidambar formosana is improved.
2. The preparation and use methods of the invention are simple and easy to operate, and fully exert the bright ornamental effect of the leaf color scarlet in autumn of the sweetgum on the premise of not influencing the normal growth of the sweetgum.
Drawings
FIG. 1 is a schematic diagram of the measurement position of the color attribute parameters of the leaves in Experimental example 1.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1:
a spraying agent for promoting the color change of sweetgum leaves comprises 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L sucrose.
The preparation method of the spraying agent comprises the following steps:
weighing, namely weighing 100mg of abscisic acid, 6mmol of citric acid, 4mmol of salicylic acid, 200mg of cane sugar and the balance of water, wherein the total volume is 1L;
and step two, mixing, namely mixing and dissolving the weighed abscisic acid, citric acid, salicylic acid and cane sugar in water to prepare the spraying agent.
The application method of the spraying agent comprises the following steps: spraying at the ratio of 16: 00-18: 00 every 10-15 days in the middle 10 th to the end 12 th of the month in the rainy-free weather, and uniformly spraying the leaf surfaces of the liquidambar formosana seedlings after the spraying agent is filled into a mist sprayer until the dropping degree of the leaves is reached every time. When the fertilizer is sprayed every time, the fertilizer is sleeved on the flowerpot of the sweetgum seedling by a plastic bag to prevent soil acidification.
Experimental example 1:
1. test materials
The test material is a 3-year-old sweetgum seedling with consistent growth vigor and good growth, and the seed source is Batouxiang in Debao county, Baise, Guangxi.
2. Design of experiments
The test is carried out in scientific research nursery base of Guangxi forestry scientific research institute in 2020, 10/15/2021, 1/25. The experiment divide into two stages, and the first stage is the blade spraying experiment, and under the spraying condition that spraying concentration is 0, the blade discolours when nearly 50%, stops the spraying experiment, and the test time in this stage is: the test factors comprise four of abscisic acid (A), citric acid (B), salicylic acid (C) and sucrose (D), wherein each factor is provided with four levels (table 1), and L is selected from L16(45) The tables were designed orthogonally (table 2). Every 10 liquidambar formosana seedlings are treated, every 10 to 15 days, and the leaf surfaces are uniformly sprayed by a mist sprayer in the rainy-free weather till the end of 12 months. The spraying liquid is sprayed to the leaf drops every time, and the plastic bag is sleeved on the flowerpot to prevent soil acidification every time of spraying. Spraying is carried out at a ratio of 16: 00-18: 00. The leaf color change ratio was recorded before each spray after the treatment began. And a second stage: after spraying, the sweetgum has obvious leaf falling tendency, the time in the experiment lasts to 1 month and 25 days in 2021 year, the stage is mainly to observe whether the leaf color still has obvious change and final color change effect after the spraying experiment, sampling is carried out according to the color change effect in the color change stable period, and the parameter of the leaf color is measured。
TABLE 1 orthogonal test factor level table
Figure RE-GDA0003184674640000031
TABLE 2 orthogonal experimental design Table
Test No A B C D
1 0 0 0 0
2 0 2 2 200
3 0 4 4 300
4 0 6 6 500
5 100 0 2 300
6 100 2 0 500
7 100 4 6 0
8 100 6 4 200
9 200 0 4 500
10 200 2 6 300
11 200 4 0 200
12 200 6 2 0
13 400 0 6 200
14 400 2 4 0
15 400 4 2 500
16 400 6 0 300
3. Measurement index
(1) Measurement of color change ratio and time: spraying was carried out according to the spraying method of example 1, and the ratio of discoloration at each level before each spraying was recorded, as well as the length of time after the final discoloration exceeded 60%.
(2) Measurement of blade parameters: randomly taking 10 leaves from branches at the middle upper part of the periphery of the crown of the sweetgum plant in different directions. Divided into 3 replicates, each replicate 5 leaves. And measuring the color attribute parameters of the leaves. The leaf color attribute parameters were determined according to the method of Sarker. The measuring instrument adopts a Spectrophotometer CS-650 Spectrophotometer with test modes of SCI and D65 light sources, 10 points of the upper, middle and lower parts of the main vein on the front surface of the leaf and the upper parts and two sides of the two side veins are selected at a measuring position with an angle of 10 degrees, the leaf veins are avoided during measurement, and L is recorded*、a*、b*And calculate c*,c*=(a*2+b*2)1/2
4. Analysis of results
(1) Different orthogonal tests combine the proportion of discoloration and the duration of discoloration of more than 60%
The leaf proportion of discolouing and the duration of discolouing can audio-visually represent the leaf effect of discolouing of sweetgum, and this test result shows: in the spraying mode of test No. 8 (the spraying agent of example 1) in the orthogonal test combination, the color of the liquidambar formosana is changed at the earliest, the time when the color change reaches 60% is the earliest, the duration is the longest, and specific results are shown in table 3.
TABLE 3 combination of the proportion of discoloration and the duration of the discoloration of more than 60% by different orthogonal tests
Figure RE-GDA0003184674640000041
(2) Combining leaf color parameter variations from different orthogonal tests
The study shows that in the leaf color parameter, L*Representing the degree of color shading, L*The larger the value, the higher the leaf color brightness; a is*、b*Is a hue parameter, a*Representing the red and green attributes of the leaf color, positive values represent red bias, and negative values represent green bias; b*The leaf color is yellow-blue, positive values indicate yellow, and negative values indicate blue; c. C*Is the color saturation or color vividness, c*The larger the value, the more brilliant the leaf color; the test result shows that: l of sweetgum leaf in spraying mode of test No. 8 (spraying agent of example 1) in orthogonal test combination*、a*、b*、 c*Is significantly larger than other orthogonal experimental combinations (P)<0.05), showing that the spraying mode has the highest brightness of the leaf color, the best reddening effect of the leaves, the most bright leaf color and stronger ornamental value of the leaves, and the specific results are shown in Table 4.
TABLE 4 different orthogonal tests combine leaf color parameter variation
Figure RE-GDA0003184674640000042
Figure RE-GDA0003184674640000051
5. Conclusion of the experiment
The results of various indexes of combining the color change proportion, the color change duration and the leaf color parameter change show that: in the No. 8 test in the orthogonal test combination, namely in the leaf spraying mode of 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L cane sugar, the leaf has the largest color change proportion, the earliest color change and the longest color change period, and meanwhile, the leaf has the highest brightness, the leaf has the best reddening effect, the leaf has the most bright color, and the leaf has stronger ornamental value, namely, the spraying agent in the embodiment 1 is the optimal spraying agent which can promote the color change of the liquidambar formosana in autumn and prolong the color change period.
Comparative example 1:
the difference from example 1 is that the sprayed object is leaves of 3-year old ginkgo seedlings which grow well. The test is carried out in scientific research nursery base of Guangxi forestry scientific research institute. The specific measures are as follows: spraying a spraying agent mixed by 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L cane sugar on ginkgo leaves at least 45 days before ginkgo leaf falling, spraying the spraying agent once at a ratio of 16: 00-18: 00 every 10-15 days in the rainy-free weather, filling the spraying agent into a mist sprayer, and uniformly spraying the leaves of ginkgo seedlings until the dropping degree of the leaves is reached every time.
Results after 5 spraying: the ginkgo leaf tablets sprayed with the spraying agent have no obvious phenomenon of yellowing in advance relative to untreated leaves, and the individual leaves are yellow in advance for 3 days at most; the color is not obviously different from that of the untreated blade; the color-changing period is not obviously different from that of untreated leaves, and the color-changing period of individual leaves is prolonged by 3 days at most. That is, the spraying agent has no effect of promoting color change or prolonging color change period of ginkgo leaves.
Comparative example 2:
the difference from the example 1 is that the sprayed object is a 3-year-old sapium sebiferum seedling with consistent growth vigor and good growth. The test is carried out in scientific research nursery base of Guangxi forestry scientific research institute. The specific measures are as follows: spraying a spraying agent mixed by 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L cane sugar on the leaves of the sapium sebiferum at least 45 days before the leaves of the sapium sebiferum fall, spraying the spraying agent once at a ratio of 16: 00-18: 00 every 10-15 days in the rainy-free weather, filling the spraying agent into a mist sprayer, uniformly spraying the leaves of sapium sebiferum seedlings, and spraying the spraying agent to the degree of dropping liquid on the leaves every time.
Results after 5 spraying: the Chinese tallow tree leaves sprayed with the spraying agent have no obvious phenomenon of early reddening relative to untreated leaves, and individual leaves are reddened at most 5 days in advance; the color is not obviously different from that of the untreated blade; the color-changing period is not obviously different from that of untreated leaves, and the color-changing period of individual leaves is prolonged by 5 days at most. That is, the spraying agent has no effect of promoting discoloration or prolonging discoloration period of leaves of Sapium sebiferum.
As can be seen from example 1 and comparative examples 1 and 2, the spraying agent is effective in promoting the leaves of sweetgum to turn red, prolonging the discoloration period, and has no obvious effect on the discoloration of ginkgo biloba and Chinese tallow tree leaves.
The above examples are merely illustrative of the embodiments of the present invention and are not to be construed as limiting the scope of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention.

Claims (4)

1. The spraying agent for promoting the color change of the sweetgum leaves is characterized in that: the raw material components and the content of the spraying agent are 100mg/L abscisic acid, 6mmol/L citric acid, 4mmol/L salicylic acid and 200mg/L sucrose.
2. The spray agent for promoting the discoloration of sweetgum leaves as claimed in claim 1, wherein: the preparation method of the spraying agent comprises the following steps:
weighing, namely weighing 100mg of abscisic acid, 6mmol of citric acid, 4mmol of salicylic acid, 200mg of cane sugar and the balance of water, wherein the total volume is 1L;
and step two, mixing, namely mixing and dissolving the weighed abscisic acid, citric acid, salicylic acid and cane sugar in water to prepare the spraying agent.
3. The spray agent for promoting the discoloration of sweetgum leaves as claimed in claim 1, wherein: the application method of the spraying agent comprises the following steps: spraying at the ratio of 16: 00-18: 00 every 10-15 days in the middle 10 th to the end 12 th of the month in the rainy-free weather, and uniformly spraying the leaf surfaces of the liquidambar formosana seedlings after the spraying agent is filled into a mist sprayer until the dropping degree of the leaves is reached every time.
4. The spray agent for promoting the discoloration of sweetgum leaves as claimed in claim 3, wherein: when the fertilizer is sprayed every time, the fertilizer is sleeved on the flowerpot of the sweetgum seedling by a plastic bag to prevent soil acidification.
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Denomination of invention: A Spraying Agent to Promote Color Change of Maple Leaf

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