CN109392496A - A method of promote zelkova schneideriana autumn red blade to deepen - Google Patents
A method of promote zelkova schneideriana autumn red blade to deepen Download PDFInfo
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- CN109392496A CN109392496A CN201811120909.5A CN201811120909A CN109392496A CN 109392496 A CN109392496 A CN 109392496A CN 201811120909 A CN201811120909 A CN 201811120909A CN 109392496 A CN109392496 A CN 109392496A
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- citric acid
- zelkova schneideriana
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- red
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of methods that promotion zelkova schneideriana autumn red blade is deepened, and the method is to carry out spraying processing with blade face of the citric acid to zelkova schneideriana autumn red blade.Citric acid, which can be used for preparing, promotes zelkova schneideriana autumn red blade to deepen preparation.
Description
Technical field
The present invention relates to a kind of methods that promotion zelkova schneideriana autumn red blade is deepened.
Background technique
Zelkova schneideriana is the tall and big deciduous tree of Ulmaceae (Ulmaceae) Zelkova, and tree-like graceful, leaf color Seasonal dynamics change enriches, and is
Excellent ornamental tree species.Be broadly divided into three classes according to its leaf color change in the fall: blade takes on a red color, blade in yellow and
Blade keeps green substantially, until withered rapidly before fallen leaves fall off (Luo Xuemei 2012).Though zelkova schneideriana Seasonal dynamics change is abundant, shadow
The reason of ringing its leaf color is numerous, and leaf color change is unstable, directly affects its application and ornamental value.At present, both at home and abroad about
Research in terms of zelkova schneideriana leaf color is less, and Liu Xuemei etc. (2014a, b) has studied 3 class colour system schneider zelkova leaf color tables up to phase pigment content
Changing rule and its growth characteristics, it is believed that red, yellow, green be zelkova schneideriana leaf color expression respectively with Anthocyanin Content, leaf
Green element is closely related with ratio, the higher chlorophyll content of carotenoid.It opens quick equal (2015) and has studied 3 kinds of different colour systems
Pigment, Mineral Elements Content, carbohydrate and its correlation in zelkova schneideriana blade, it is believed that the original of autumn zelkova schneideriana leaf color change
Because being that pigment ratio and ingredient are changed, and its important inside and outside factor is temperature, sugar, mineral element etc..Temporarily at present
The method that Shi Wei has research to seek regulation zelkova schneideriana leaf color.Ducamp-Collin etc. makes lichee pericarp studies have shown that spraying citric acid
In anthocyanidin it is more stable, storage 3 weeks after pericarp it is still colour-fast.It is applied in nutrient solution test to the outer of poplar ' in red poplar ',
It was found that under different illumination conditions, anthocyanidin content in blade can significantly improve 3% citric acid；With 0.2% citric acid
Purple Ye Bitao, prunus cerasifera, Prunus cistena and beauty plum are sprayed, is also promoted the anthocyanidin content in blade.Zheng Xuchen etc.
It grinds to make internal disorder or usurp and finds that the citric acid of 4.8mmol/L can be such that the gray wool smoke tree discoloration phase shifts to an earlier date, the effect that reddens enhancing, while citric acid is one again
The naturally occurring organic acid of kind, the influence to environment is smaller, therefore, can use external source in practice and sprays citric acid to improve
The appreciation effect of gray wool smoke tree.
Summary of the invention
The present invention is directed to overcome the deficiencies of the prior art and provide a kind of method that promotion zelkova schneideriana autumn red blade is deepened.
The present invention has studied various concentration citric acid to 3 kinds of difference colour system zelkova schneideriana leaf color changes of red, yellow and green
It influences, finds the effective way for improving zelkova schneideriana leaf color, explore the method for artificial regulatory zelkova schneideriana leaf color.
The result shows that:
The citric acid for spraying 9.6 mmol/L can accelerate the decomposition of Chlorophyll in red colour system zelkova schneideriana blade, remarkably promote red colour system
The synthesis of schneider zelkova leaf piece anthocyanidin deepens red colour system zelkova schneideriana blade red degree, and the discoloration phase shifts to an earlier date, and about in advance 15 days；It is different
Concentration citric acid treatment can extend the green period of yellow class zelkova schneideriana, and blade is made to maintain green, and the discoloration phase postpones, and about postpone 12
It；Citric acid is that the influence of zelkova schneideriana leaf color is not significant on green.
Therefore, citric acid, which can be used for preparing, promotes zelkova schneideriana autumn red blade to deepen preparation, and when application is by citric acid pair
The blade face of zelkova schneideriana autumn red blade carries out spraying processing, and the concentration of the citric acid preferably sprayed is 9.6 mmol/L.
Specific embodiment
One, materials and methods:
1 test material: Sino-South African Forestry University of Science and Technology, base, nursery, selection growing way is consistent, well-grown, divides after autumn blade annesl
Not Wei red, yellow, green 1 year raw zelkova schneideriana grafting be test material, its blade face is carried out using citric acid to spray processing,
Every 4 plants, 4 plants of control group of processing.It applies 0,4.8,9.6 and 14.4mmol/L citric acid, and clear water processing is control.From 2015
Start to spray processing within September 20 days, uniformly be sprayed with small-sized watering can on blade two sides, every 5d is sprayed once, continues 30d.In zelkova schneideriana
The discoloration phase (October 1 to November 6), every 6 sampling is primary, samples 7 times altogether.The mature leaf in the middle part of plant is taken at random, is put
Enter to take back laboratory in valve bag and carry out leaf color measurement, reject master pulse later, with liquid nitrogen grinding, carries out chlorophyll and anthocyanidin contains
The measurement of amount.
2 test methods
(1) leaf color measures:
Using International Commission on Illumination (International Commission on Illumination, CIE) colour examining standard
Carry out leaf color measurement.After collected fresh leaf is wiped clean, existed with light splitting colour difference meter (MINOLTA CR-410, Japanese Konica)
Leaf color is measured under C/2 ° of light source, every leaf measures 3 positions.In CIEL ﹡ a ﹡ b ﹡ colour system, L ﹡ value indicates the size of lightness, color
Phase a ﹡ value is indicated from green to red variation；And form and aspect b ﹡ value indicates variation from blue to yellow, by measurement brightness value,
Form and aspect a ﹡ value and b ﹡ value calculate chroma C ﹡ value, C ﹡=(a ﹡2+ b ﹡2)1/2, the vertical range of C* value expression to L ﹡ axis, away from
From bigger, chroma is bigger.
(2) pigment content measures: method of the measurement of anthocyanidin relative amount and chlorophyll content referring to Tang Qianrui etc..
(3) data are analyzed: being carried out Data Analysis Services using Excel 2007 and SPSS 19, made using 8.0 software of Origin
Figure.
Two, result and analysis
1, influence of the citric acid to 3 kinds of colour system zelkova schneideriana blade form and aspect of red, yellow, and green
(1) influence that various concentration citric acid changes 3 kinds of colour system zelkova schneideriana form and aspect a ﹡ values of red, yellow, and green
Influence of the various concentration citric acid treatment to 3 kinds of colour system zelkova schneideriana form and aspect a ﹡ values is different.Red colour system zelkova schneideriana is 9.6
Under mmol/L citric acid treatment, red degree a ﹡ value is above control group, and test period is more long, and a ﹡ value more tends towards stability.
Illustrate red colour system zelkova schneideriana blade red degree can be made to deepen by spraying 9.6 mmol/L citric acids.Yellow class zelkova schneideriana is in each concentration
Under citric acid treatment, form and aspect a ﹡ value is below control, and the variation of a ﹡ value is inhibited by certain, and at 9.6 mmol/L citric acids
The blade of reason its a ﹡ value during test is below 4.8 mmol/L citric acids.Illustrate that various concentration citric acid treatment can extend
The green period of yellow class zelkova schneideriana makes blade maintain green, and the discoloration phase postpones, in low concentration (0-9.6 mmol/L) range, lemon
Lemon acid concentration is higher, and blade is greener.Green is zelkova schneideriana under citric acid treatment, and the variation of form and aspect a ﹡ value is not significant.
(2) influence that various concentration citric acid treatment changes 3 kinds of colour system zelkova schneideriana form and aspect b ﹡ values of red, yellow, and green
Under various concentration citric acid treatment, red colour system zelkova schneideriana b ﹡ value is integrally on a declining curve, and yellow class zelkova schneideriana b ﹡ value is whole bent
Folding rises, and green is that the variation of zelkova schneideriana b ﹡ value is relatively steady.Leaf color b ﹡ of the various concentration citric acid treatment to 3 kinds of colour system zelkova schneiderianas
It is not significant to be worth changing rule.
2, influence of the citric acid to 3 kinds of colour system zelkova schneideriana anthocyanidin relative amounts of red, yellow, and green
Under 4.8,9.6mmol/L citric acid treatment, anthocyanidin relative amount significantly increases red colour system zelkova schneideriana compared with control group,
November 6 daily reaches maximum value, 1.76 respectively compareed times and 1.66 times.And under 14.4mmol/L citric acid treatment, it is red
Colour system schneider zelkova leaf piece anthocyanidin content, which is substantially less than, to be compareed.Illustrate through foliage-spray low concentration (4.8 and 9.6mmol/L) lemon
Acid can remarkably promote the synthesis of red colour system schneider zelkova leaf piece anthocyanidin, and citric acid concentration is excessively high (14.4mmol/L), then inhibits it
The synthesis of anthocyanidin.Yellow class zelkova schneideriana is under various concentration citric acid treatment, and blade anthocyanidin content and control group are without obvious
Difference.Green is zelkova schneideriana under various concentration citric acid treatment, and anthocyanidin content slightly rises compared with control group.Explanation sprays
Various concentration citric acid, can promote green to a certain extent is the synthesis of schneider zelkova leaf piece anthocyanidin, but its with compare between difference
Not significant (P > 0.05).
3, influence of the citric acid to 3 kinds of colour system zelkova schneideriana plamochromic pigment contents of red, yellow, and green
(1) influence of the various concentration citric acid to Chlorophyll content in 3 kinds of colour system zelkova schneideriana blades of red, yellow, and green
Various concentration citric acid has certain inhibiting effect in the later period to the chlorophyll of red colour system zelkova schneideriana.Wherein, exist
Under 9.6mmol/L citric acid treatment, red colour system zelkova schneideriana leaf chlorophyll is consistently lower than control group.Illustrate 9.6mmol/L citric acid
The decomposition for promoting Chlorophyll in red colour system zelkova schneideriana blade, shifts to an earlier date red colour system zelkova schneideriana viewing period.Various concentration citric acid is to Huang
Color, green are that the influence of zelkova schneideriana leaf chlorophyll is not significant.
(2) influence of the various concentration citric acid to carotenoid content in 3 kinds of colour system zelkova schneideriana blades of red, yellow, and green
The citric acid of various concentration to red, yellow, green be zelkova schneideriana carotenoid content influence it is not significant.Control
Under group, 14.4mmol/L citric acid treatment, green is that the carotenoid content of zelkova schneideriana is all remarkably higher than red colour system zelkova schneideriana, and right
It is that carotenoid content is gradually increased with color change in zelkova schneideriana blade, and is sprayed at citric acid according to a group red, yellow, green
Red, yellow, green after reason are the carotenoid content of zelkova schneideriana with color change and this not shown regularity.Illustrate, lemon
Lemon acid has certain interference to the formation of carotenoid in different colour system zelkova schneideriana blades.
Claims (5)
1. a kind of method for promoting zelkova schneideriana autumn red blade to deepen, which is characterized in that the method is with citric acid to zelkova schneideriana
The blade face of autumn red blade carries out spraying processing.
2. the method as described in claim 1, which is characterized in that the concentration of the citric acid sprayed is 9.6 mmol/L.
3. citric acid promotes zelkova schneideriana autumn red blade to deepen the application in preparation in preparation.
4. application as claimed in claim 3, which is characterized in that the application is with citric acid to zelkova schneideriana autumn red blade
Blade face carries out spraying processing.
5. application as claimed in claim 4, which is characterized in that the concentration of the citric acid sprayed is 9.6 mmol/L.
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Cited By (2)
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| CN113396908A (en) * | 2021-06-17 | 2021-09-17 | 广西壮族自治区林业科学研究院 | Spraying agent for promoting color change of sweetgum leaves |
| CN115322042A (en) * | 2022-09-14 | 2022-11-11 | 重庆市林业科学研究院 | Cotinus coggygria leaf color regulating agent and using method thereof |
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| CN113396908A (en) * | 2021-06-17 | 2021-09-17 | 广西壮族自治区林业科学研究院 | Spraying agent for promoting color change of sweetgum leaves |
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