CN108452079B

CN108452079B - Methods and compositions for delaying senescence, inhibiting tumor cell growth, and improving microcirculation

Info

Publication number: CN108452079B
Application number: CN201810703893.4A
Authority: CN
Inventors: 傅金荣
Original assignee: Jiangxi Tianyuan Medicine Co ltd
Current assignee: Jiangxi Tianyuan Medicine Co ltd
Priority date: 2018-07-01
Filing date: 2018-07-01
Publication date: 2021-03-26
Anticipated expiration: 2038-07-01
Also published as: CN108452079A

Abstract

The present invention relates to methods and compositions for delaying senescence, inhibiting tumor cell growth, and improving microcirculation. The composition of the present invention is made from a material selected from the group consisting of: 8 parts of ginseng, 4-8 parts of cordyceps militaris, 4-8 parts of prepared rehmannia root, 4-8 parts of epimedium herb, 1-3 parts of cistanche, 1-3 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier. The composition is typically in the form of an oral liquid. The invention also relates to a preparation method of the composition and application of the composition in preparing products for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation. The composition has excellent effects of delaying senility, inhibiting the growth of tumor cells and improving cardiovascular and cerebrovascular microcirculation, and has the excellent effects of high yield of active substances and good stability of the active substances.

Description

Methods and compositions for delaying senescence, inhibiting tumor cell growth, and improving microcirculation

Technical Field

The invention belongs to the technical field of medical health products, relates to a composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation, also relates to a preparation method of the composition and medical and medicinal applications of the composition, and more particularly relates to a composition prepared from ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche deserticola and cervus elaphus linnaeus.

Background

Aging refers to the phenomenon that the physiological and psychological adaptive capacity of the body to the environment is gradually reduced and gradually dies. Aging can be divided into two categories: physiological aging and pathological aging. The former refers to a physiological degenerative process occurring after maturation and the latter is an aging change due to various external factors including various diseases. The two are practically indistinguishable. In summary, aging is a corollary of the combined effects of many pathological, physiological and psychological processes, the biological psychological process of the final stages of growth and development of an individual.

Biologically, aging is a spontaneous inevitable process of an organism over time, and is a complex natural phenomenon, which is manifested as degeneration of structure and functional decline, and decline of adaptability and resistance. Physiologically, aging is considered as a history of ontogeny proceeding from the fertilized egg to the elderly. Pathologically, aging is the result of stress and strain, injury and infection, decline in immune response, nutritional imbalance, metabolic disorders, and the accumulation of negligence and abused drugs. In addition, from the sociology, the aging is that the individual loses interest in fresh things, is out of reality and likes nostalgic.

One of the activities accompanying the life development and development process is the loss and degeneration of the body from the constituent materials and tissue structures to physiological functions. The aging process is continued from the fertilized egg to death, and the aging characteristics are only obviously shown until a certain stage. The physiological changes in the aging process of human body are mainly reflected in the loss of organism tissue cells and constitutional substances, the slowing of organism metabolic rate and the hypofunction of organism and organs. Senescence is inevitable, but delaying senescence is possible. The reasonable diet and the balanced nutrition are one of the important measures for delaying the aging and prolonging the life. Aging is characterized mainly by: (1) there is a time dependence; (2) is a ubiquitous phenomenon of all cells, tissues, organs and the whole body in a living body; (3) is an endogenous change caused by natural trends.

The main physiological manifestations of aging are: (1) and (4) changing the form. The method comprises the following steps: (ii) a cellular change. Mainly manifested as a gradual decrease in cell number. Variation of tissues and organs. Atrophy and weight loss occur due to the decrease in the number of cells in internal organs and tissues. And integral change. Changes in body shape and appearance, such as hair whitening, occur with age; the elasticity of the skin is reduced, wrinkles appear, and age spots appear; loose and fallen teeth, deafness, dim eyesight, hunchback, and gradually shortened height. (2) Physiological function declines. The method comprises the following steps: decline of cardiovascular system function. For example, the cardiac fibers gradually shrink, the senile pigment (lipofuscin) in the cardiac muscle cells is deposited, and the cardiac valves become thick and hard, the elasticity is reduced, and the like. ② the aging of respiratory organs. It is manifested as a decrease in lung volume, a significant decrease in respiratory function, and a decrease in compensatory ability. ③ changes in the digestive system. Mainly, the function of the oral cavity and the stomach and intestine is weakened, and the gingiva and the teeth are atrophically changed. Fourthly, the variation of the musculoskeletal movement system. Mainly characterized by the thinning of muscle fiber, the reduction of elasticity and the weakening of contractility with the age; the organic components in the bone are reduced, and the inorganic salt is increased, so that the flexibility of the elasticity of the bone is reduced, and the bone is easy to fracture. Nervous system changes. Mainly manifested as some degree of loss of brain cells; a decrease in nerve conduction velocity; the elderly have sluggish movements, reduced response dexterity, etc. (3) The main sensory organ functions decline. Such as visual, auditory, olfactory, gustatory, and cutaneous sensory (including tactile, temperature, and pain) abilities. In addition, mental motor responses of the elderly are correspondingly sluggish.

Aging itself has 6 major characteristics. The universality is as follows: i.e., all organisms will age. Second, inherent property: the aging process is a spontaneous inevitable process in the body, i.e., the aging process is gradually progressed even when living in an optimum environment. ③ progressive: aging is a process that continues to progress over time. Harmfulness: aging reduces the physiological functions of the organism, increasing the chances of illness and death. Individual difference: the aging process varies among individuals within the same organism, and this difference is more pronounced, particularly later in life. Longevity is only possible in those individuals who are slow aging. Interference: although aging is an inherent, spontaneous process, external conditions can accelerate or retard the progression of this process. Such as ambient temperature, can alter the longevity of the animal. Therefore, it is possible to improve the living environment and to prolong the life. Physiologically, aging is caused by hypometabolism. Metabolism is one of the basic features of life activities, and includes both anabolism and catabolism. If the anabolism of the organism is higher than the catabolism, the human body can grow and develop, namely childhood and juvenile; if the speeds of the two metabolic processes are basically balanced, the human body reaches the middle age and the strong age, and the change of the human body is small in the middle age and the strong age; if catabolism is higher than anabolism, a person begins to age, and if metabolism ceases, the person's vital activities end. Such changes are ubiquitous throughout a person's lifetime, and thus aging is inevitable. However, in a person's lifetime, the aging process occurs early, either for intrinsic or extrinsic reasons, which is premature aging. Premature aging is pathological aging that affects the life of a person. Pathological aging is likely to occur in the elderly, but not necessarily in every person. Physiological aging is therefore an inevitable natural law, whereas pathological aging is prevented and delayed. In order to combat aging, the mechanisms of aging have been extensively studied. However, the mechanism of aging has not been completely understood so far.

The tumor cell parenchyma is a tumor. The tumor tissue is composed of parenchyma and stroma, and the tumor parenchyma is tumor cells, is a main component of the tumor and has tissue source specificity. It determines the biological characteristics of the tumor and the specificity of each tumor. The tissue origin of various tumors is generally identified according to the parenchymal morphology of the tumor, classification, naming and histological diagnosis of the tumor are performed, and the benign and malignant degree of the tumor and the malignant degree of the tumor are determined according to the differentiation maturity degree and the size of the heterogeneity. Tumor cells have three significant basic features: immobility, migration and loss of contact inhibition. In addition, tumor cells have many physiological, biochemical and morphological features that differ from normal cells.

Tumors (tumors) are a genetic disease, but are not inherited. It means that under the action of tumorigenic factors, the gene of the cell is changed, and the normal regulation and control of the growth of the cell are lost, so that abnormal hyperplasia is caused. Can be divided into two categories, benign and malignant tumors. The former has slow growth, clear boundary with surrounding tissues, no transfer and little harm to human health. The latter grows rapidly, can be transferred to other parts of the body, can also produce harmful substances, damage normal organ structures, cause organism dysfunction and threaten life. The tumor tissue is composed of parenchyma and stroma, and the tumor parenchyma is tumor cells, is a main component of the tumor and has tissue source specificity. The tumor stroma, which serves to support and nourish the tumor parenchyma, is not specific and generally consists of connective tissue and blood vessels, and sometimes lymphatic vessels.

Tumor cells have three significant basic features: immobility, migration and loss of contact inhibition. In addition, cancer cells have many physiological, biochemical and morphological features that are different from normal cells. Tumor cells are different in size and form, generally larger in size than the original cells, and high in growth speed, and have a nuclear-to-cytoplasmic ratio which is significantly higher than that of normal cells and can reach 1:1, the normal differentiated nucleus-to-cytoplasm ratio is only 1: 4-6. The cores have different shapes and can have the phenomena of megakaryokinesis, binuclear or multinuclear. The nuclear chromosomes are in an aneuploidy state (aneuploidy), some of the chromosomes are deleted, and some of the chromosomes are increased in number. Abnormal changes in normal cell chromosomes initiate the apoptotic process, but in cancer cells, apoptosis-related signaling pathways become dysfunctional, i.e., cancer cells are immortal. Mitochondria exhibit different polytypes, swelling, hyperplasia, such as hypertrophic mitochondria in eosinophilic adenomas are tightly packed inside the cell, and giant mitochondria appear in hepatoma cells. Cytoskeletal disturbance, a decrease in certain components, abnormal assembly of the skeleton. Cell surface characteristics are altered, producing tumor associated antibodies (tumorassociatedigien).

Generally, various tumors, patients with body fluid impairment and yin robbery are mostly yin deficiency with internal heat, so pungent, warm and dry heat foods should be avoided in diet regulation, and less eating is recommended for greasy food; in the middle and late stages of cancer, deficiency syndrome and cold syndrome are the main symptoms, and foods such as warming and invigorating spleen and stomach, tonifying qi and generating blood are advocated on diet, while foods with cold and cool nature should be eaten little or not. The diet contraindication of cancer patients is different in different disease categories, for example, patients with nasopharyngeal carcinoma should avoid pungent, warm, dry and hot, fried and roasted food, dog meat, mutton, pepper, fennel, etc. during the radiotherapy period; patients with gastric cancer are prohibited from eating pungent and dry food, cassia bark, mustard, pepper and the like; old pork and old duck are forbidden for patients with esophageal cancer; mother pork and few leeks are forbidden for patients with liver cancer. In a word, proper diet should be avoided, and blindly avoiding diet will cause malnutrition and affect recovery from diseases. The life must be maintained by various nutrients in the food. The most important problem of cancer patients is nutrition disorder, and improving the nutrition of patients is the most important measure in anticancer treatment: (1) it can be used for protein food such as milk, fish, egg, and bean product, and has anticancer effect. Wherein the milk and egg can improve protein disorder after radiotherapy. (2) Proper amount of sugar is taken to supplement heat. When a patient is treated by a large dose of radiation, the sugar metabolism in the body of the patient is destroyed, the glycogen is rapidly reduced, and the lactic acid in the blood is increased and can not be reused; and insulin insufficiency worsens. Therefore, the effect of supplementing glucose is better, and in addition, honey, rice, flour, potatoes and other foods with rich sugar are more suitable to be eaten to supplement heat. (3) More foods with anticancer effect, such as Amyda sinensis, Agaricus campestris, Auricularia, Bulbus Allii, Sargassum, and caulis et folium Brassicae Junceae, etc., can be eaten. (4) Patients with radiotherapy and chemotherapy should eat cold food and cold drink, but patients with cold feeling should eat hot food. (5) Researches show that cordycepin contained in the cordyceps sinensis can effectively phagocytize tumor cells, has the effect of 4 times of selenium, can enhance the capability of red blood cells to adhere to the tumor cells, and can play a role in preventing tumor recurrence and metastasis during tumor chemotherapy and after tumor surgery. (6) The food is diversified, and the color, the fragrance and the taste are noticed, so that the appetite of the patient is promoted; the cooking food is mostly cooked, boiled and stewed, and the food which is difficult to digest is contraindicated, and the drinking is forbidden. (7) Vitamins A and C have the effects of preventing cell degeneration and diffusion, and increasing epithelial cell stability, and vitamin C can also prevent common symptoms of radiation injury, and can increase leukocyte level; vitamin E can promote cell division and delay cell aging; vitamin B1 can stimulate appetite and relieve symptoms caused by radiotherapy. Therefore, foods rich in the above vitamins, such as fresh vegetables, fruits, sesame oil, cereals, beans, and animal viscera, should be eaten more.

Microcirculatory disturbance is the chief culprit of cardiovascular and cerebrovascular diseases. Cerebrovascular accidents are common diseases seriously threatening the health of human beings, particularly the health of middle-aged and elderly people over 50 years old, the patients are younger, about 40 years old are gradually increased, even if the patients are in twenty years old, the patients have slight diseases, even if the most advanced and perfect treatment means are applied, more than 50 percent of survivors of the cerebrovascular accidents still can not take care of the lives! In China alone, nearly 300 million people die of cardiovascular and cerebrovascular diseases every year, about 8200 people every day, about 340 people every hour and about 6 people every minute. Therefore, the cardiovascular and cerebrovascular diseases become a big killer to harm human health. In common words: microcirculation is smooth, and stroke cannot be caused; good microcirculation and less myocardial infarction; the microcirculation is smooth, and the whole body is healthy. "microcirculation is almost the source of all diseases, and healthy microcirculation function is the first prerequisite to ensure the vital organs in the body to perform normal functions. Microcirculation refers to the circulation of blood between the arteriovenous and venules that are directly involved in the exchange of metabolic substances between tissues and cells. It directly supplies blood, oxygen, energy and relative nutrient substances to cells, and also discharges the metabolites harmful to human body, such as creatine, lactic acid, carbon dioxide, etc., so that the microcirculation is the place of human body metabolism, is the internal environment of human body, and is the most basic guarantee of life. It is generally considered that the occurrence of cerebral apoplexy is the result of long-term action of various risk factors such as hypertension, heart disease, cerebral arteriosclerosis, obesity and the like, and actually, deep causes are inseparable from the microcirculation disturbance of human body. The microcirculation of human body directly participates in metabolism and material exchange of cells, directly supplies blood, oxygen and energy and related nutrients to the cells, and simultaneously carries out the metabolites harmful to human body, such as creatine, lactic acid, carbon dioxide and the like. If platelets, leukocytes or fibrin in the microcirculation are mutually aggregated to form microthrombi, they are moved to the whole body with the blood flow or deposited on the intima of blood vessels, and then cause a series of symptoms when the plugs cause local blood flow obstruction. For example, when microcirculation disturbance occurs in the nervous system, brain cells cannot obtain enough nutrition and oxygen, and cell metabolites cannot be completely discharged out of the body due to insufficient blood supply, so that headache, dizziness, insomnia, dreaminess and memory deterioration can be caused; the serious patients have apoplexy, hemiplegia, senile dementia, peripheral neuritis, etc. When microcirculation disturbance occurs in the cardiovascular system, chest distress, palpitation, angina pectoris and arrhythmia are caused, and myocardial infarction is caused in serious patients. The microcirculation disturbance of other systems of the human body can cause corresponding symptoms.

Microcirculation is the circulation of blood in the capillaries between arterioles and venules, and is the most basic structural and functional unit of the circulatory system. It includes the circulation of body fluids within arterioles, venules, lymphatic capillaries and tissue tracts. Each organ and each tissue cell of the human body are supplied with oxygen and nutrients by microcirculation, and energy is transmitted, information is exchanged, and carbon dioxide and metabolic waste are removed. Microcirculation disturbance is the change of the physicochemical properties of blood, so that a lumen is narrowed, the blood flow rate is slowed or thrombus is formed, ischemia, anoxia or even necrosis of local tissues is caused, a series of clinical symptoms are caused, the microcirculation is smooth, and all diseases are not generated, and the microcirculation disturbance is the source of all diseases. The healthy microcirculation function is the first prerequisite to ensure the internal organs to execute normal functions, and the medicine proves that: the human body aging, the occurrence of tumors, hypertension, diabetes, a plurality of cardiovascular and cerebrovascular diseases and the like are mainly caused by microcirculation disturbance, so whether the microcirculation is normal or not is an important sign for the health of the human body. Today lifestyle, diet, stress, pollution, etc. are all major external causes of microcirculatory disturbance. With age, the cells and blood of the human body gradually age, and the function naturally deteriorates, which is an internal cause of microcirculatory disturbance. Microcirculation disturbance can cause sub-health of many people, and in severe cases, the disturbance can cause myocardial infarction, stroke and other serious diseases.

Those skilled in the art would still expect new methods to delay aging, inhibit tumor cell growth, and improve cardiovascular and cerebrovascular microcirculation; in particular, the new composition is expected to have the application of health care, medical treatment and medicine, and is used for delaying senility, inhibiting the growth of tumor cells and improving cardiovascular and cerebrovascular microcirculation.

Disclosure of Invention

The invention aims to provide a new method for delaying senility, inhibiting the growth of tumor cells and improving cardiovascular and cerebrovascular microcirculation; another objective of the invention is to provide a new medicine or health-care product for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation. It has been surprisingly found that the compositions obtained by the formulation of the present invention exhibit excellent functions of delaying senescence, inhibiting tumor cell growth, improving cardiovascular and cerebrovascular microcirculation, and exhibit one or more excellent technical effects. The present invention has been completed based on this finding.

To this end, the invention provides, in a first aspect, a composition for delaying aging, inhibiting tumor cell growth, and improving cardiovascular and cerebrovascular microcirculation, which is prepared from a substance selected from the group consisting of: ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche deserticola, cervus elaphus linnaeus and optional physiologically acceptable carriers.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is prepared from the following substances: 8 parts of ginseng, 4-8 parts of cordyceps militaris, 4-8 parts of prepared rehmannia root, 4-8 parts of epimedium herb, 1-3 parts of cistanche, 1-3 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is prepared from the following substances: 8 parts of ginseng, 5-7 parts of cordyceps militaris, 5-7 parts of prepared rehmannia root, 5-7 parts of epimedium herb, 1.5-2.5 parts of cistanche, 1.5-2.5 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is prepared from the following substances: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The ginseng of the invention is the dried root and rhizome of the Araliaceae plant ginseng Panax ginseng C.A.Mey. Collected in autumn, washed, dried in the sun or baked. The cultivation is commonly called as 'Yuanshen'; seeding the wild mountain ginseng in the mountain forest to grow naturally is called as "mountain ginseng under forest" and is called as "seed sea". The ginseng or the extract thereof or the typical saponin in the composition of the invention comprises ginsenoside Rg1(C42H72O14), ginsenoside Re (C48H82O18) and ginsenoside Rb1(C54H92O23), and the content measurement of the three ginsenosides can be carried out by referring to the method under the content measurement item of the ginseng carried on page 8 of the first part of Chinese pharmacopoeia 2015 edition.

The composition for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation according to the first aspect of the invention is in the form of tablets or granules or oral liquid.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid, and the physiologically acceptable carrier comprises xylitol.

The composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation according to the first aspect of the invention is in the form of oral liquid, and the amount of xylitol is 6-10 parts by weight, such as 7-9 parts by weight, such as 8 parts by weight, per 8 parts by weight of ginseng.

The composition for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation according to the first aspect of the invention is in the form of oral liquid, and the dosage of ginseng in the oral liquid is 6-10 g, such as 7-9 g, such as 8g, per 100 ml.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid and is prepared by the following steps:

(1) decocting 5-8 times of ginseng in water for 1-1.5 hours, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; mixing the filtrates, concentrating to one third of the volume, adding ethanol until the ethanol content reaches 70-75%, mixing uniformly, and standing for 24 hours; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use;

(2) soaking radix rehmanniae preparata, herba epimedii, herba cistanche and cervus elaphus linnaeus in 5-8 times of water for 12 hours, then decocting and extracting for 1-1.5 hours, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; mixing the filtrates, concentrating to one third of the volume, adding ethanol until the ethanol content reaches 70-75%, mixing uniformly, and standing for 24 hours; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use;

(3) uniformly mixing cordyceps militaris, 2-3 times of xylitol and 3 times of water by weight, homogenizing until the mixture can pass through a 120-mesh stainless steel net, placing the slurry at 50 ℃ for 8-12 hours, mixing the slurry with the clear paste obtained in the step (1) and the step (2), dissolving the mixture with 30% of water by using the formula amount, and uniformly stirring;

(4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid.

The composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation according to the first aspect of the invention, wherein the prescribed amount of water is the amount of water which enables the dosage amount of ginseng to be 6-10 g, such as 7-9 g, such as 8g, per 100ml of the oral liquid.

Further, the second aspect of the present invention provides a method for preparing a composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation, wherein the composition is in the form of oral liquid, and comprises: ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche deserticola, cervus elaphus linnaeus and xylitol, and the method comprises the following steps:

The method according to the second aspect of the present invention, wherein the composition for delaying senescence, inhibiting growth of tumor cells, and improving cardiovascular and cerebrovascular microcirculation is prepared from a substance selected from the group consisting of: 8 parts of ginseng, 4-8 parts of cordyceps militaris, 4-8 parts of prepared rehmannia root, 4-8 parts of epimedium herb, 1-3 parts of cistanche, 1-3 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The method according to the second aspect of the present invention, wherein the composition for delaying senescence, inhibiting growth of tumor cells, and improving cardiovascular and cerebrovascular microcirculation is prepared from a substance selected from the group consisting of: 8 parts of ginseng, 5-7 parts of cordyceps militaris, 5-7 parts of prepared rehmannia root, 5-7 parts of epimedium herb, 1.5-2.5 parts of cistanche, 1.5-2.5 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The method according to the second aspect of the present invention, wherein the composition for delaying senescence, inhibiting growth of tumor cells, and improving cardiovascular and cerebrovascular microcirculation is prepared from a substance selected from the group consisting of: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

The method according to the second aspect of the present invention, wherein the ginseng is extracted by a water extraction and alcohol precipitation process to obtain a ginseng extract containing ginsenoside Rg1(C42H72O14), ginsenoside Re (C48H82O18), ginsenoside Rb1(C54H92O23) in a total amount of not less than 10.0%.

The method according to the second aspect of the present invention, wherein the composition for delaying aging, inhibiting tumor cell growth, and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid, and the amount of xylitol is 6 to 10 parts by weight, such as 7 to 9 parts by weight, such as 8 parts by weight, per 8 parts by weight of ginseng.

The method according to the second aspect of the present invention, wherein the prescribed amount of water is an amount such that the input amount of ginseng per 100ml of the oral liquid is 6-10 g, such as 7-9 g, such as 8 g.

Furthermore, the third aspect of the invention provides a composition prepared from ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche salsa, cervus elaphus linnaeus and optional physiologically acceptable carriers, and application of the composition in preparing products for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation.

According to the use of the third aspect of the invention, the composition is made from a material selected from the group consisting of: 8 parts of ginseng, 4-8 parts of cordyceps militaris, 4-8 parts of prepared rehmannia root, 4-8 parts of epimedium herb, 1-3 parts of cistanche, 1-3 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

According to the use of the third aspect of the invention, the composition is made from a material selected from the group consisting of: 8 parts of ginseng, 5-7 parts of cordyceps militaris, 5-7 parts of prepared rehmannia root, 5-7 parts of epimedium herb, 1.5-2.5 parts of cistanche, 1.5-2.5 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

According to the use of the third aspect of the invention, the composition is made from a material selected from the group consisting of: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus and an optional physiologically acceptable carrier.

According to the third aspect of the invention, the ginseng is extracted by a water extraction and alcohol precipitation process to obtain the ginseng extract, wherein the total content of ginsenoside Rg1(C42H72O14), ginsenoside Re (C48H82O18) and ginsenoside Rb1(C54H92O23) is not less than 10.0%.

The use according to the third aspect of the invention, wherein the composition for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of tablets or granules or oral liquid.

The use according to the third aspect of the present invention, wherein the composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid, and the amount of xylitol is 6 to 10 parts by weight, such as 7 to 9 parts by weight, such as 8 parts by weight, per 8 parts by weight of ginseng.

The use according to the third aspect of the invention, wherein the composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid, and the dosage of ginseng in the oral liquid is 6-10 g, such as 7-9 g, such as 8g of water per 100ml of the oral liquid.

The use according to the third aspect of the present invention, wherein the composition for delaying senescence, inhibiting growth of tumor cells, and improving cardiovascular and cerebrovascular microcirculation is in the form of an oral liquid, and is prepared by a method comprising the steps of:

The use according to the third aspect of the present invention, wherein the composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is in the form of oral liquid, wherein the prescribed amount of water is such that the dosage amount of ginseng per 100ml of the oral liquid is 6-10 g, such as 7-9 g, such as 8 g.

In describing the method steps of the present invention, although the particular steps described are distinguished in some detail or language from the steps described in the examples of the detailed description which follow, those skilled in the art can nevertheless fully appreciate the above-described method steps from the detailed disclosure throughout the present application.

Any embodiment of any aspect of the invention may be combined with other embodiments, as long as they do not contradict. Furthermore, in any embodiment of any aspect of the invention, any feature may be applicable to that feature in other embodiments, so long as they do not contradict.

The invention is further described below.

All documents cited herein are incorporated by reference in their entirety and to the extent such documents do not conform to the meaning of the present invention, the present invention shall control. Further, the various terms and phrases used herein have the ordinary meaning as is known to those skilled in the art, and even though such terms and phrases are intended to be described or explained in greater detail herein, reference is made to the term and phrase as being inconsistent with the known meaning and meaning as is accorded to such meaning throughout this disclosure.

Ginseng (Panax ginseng C.A.Mey) is a perennial herbaceous plant which is fond of shade and cool, has no pores and fence tissues on leaves, cannot retain water, can burn leaves at a temperature higher than 32 ℃, and has a canopy density of 0.7-0.8. Usually, the flower blossoms in 3 years, the fruits come out in 5-6 years, the flowering period is 5-6 months, and the fruit period is 6-9 months. The piny-broadleaf mixed forest or deciduous-broadleaf forest mainly comprising Korean pine, which grows at an altitude of several hundreds of meters between 33 and 48 degrees north latitude, is produced in northeast, korea, japan, and eastern russia of china. Ginseng is also called Huangshen, Dijing, Shencao and Baicao king, and is one of the three evergreen plants. Ginseng has a great economic value in the aspect of beauty. Ginseng is a best product for nourishing yin, tonifying life and strengthening body resistance, contains various saponins and polysaccharide components, can be slowly absorbed by skin without adverse stimulation, can expand skin capillary vessels, promote skin blood circulation, increase skin nutrition, regulate water-oil balance of skin, prevent skin dehydration, hardening and wrinkling, and can inhibit the reduction performance of melanin by ginseng active substances, so that the skin is white and smooth, the skin elasticity can be enhanced, cells can be regenerated, and the ginseng skin-care cosmetic is a best product for skin care and beauty. Soaking Ginseng radix directly in 50% glycerol for 10 days, rubbing face with glycerol, or decocting Ginseng radix to obtain concentrated juice, pouring a little of the concentrated juice into face washing water every day, and rubbing face with glycerol containing Ginseng radix or washing face with Ginseng radix water to moisten skin. Ginseng has great economic value in medicinal aspect. The succulent root of ginseng is a well-known tonic, is suitable for regulating blood pressure, recovering heart function, neurasthenia and asthenia, and also has expectorant, stomach invigorating, diuretic and exciting effects. It can be divided into American ginseng, northeast China ginseng and Korean ginseng according to the producing area. The transverse striation of the ginseng surface of the same variety is more obvious than that of the ginseng surface of the same variety due to different climates, and the content of effective components of imported ginseng is also higher. Nature and taste: sweet and slightly bitter in taste, warm and mild in nature. It enters spleen, lung and heart meridians. Tonify qi, secure collapse, promote fluid production, induce tranquilization, and promote intelligence. Meridian tropism: enter spleen, lung and stomach meridians. The efficacy is as follows: tonify primordial qi, recover pulse to stop collapse, tonify spleen and lung, promote fluid production, and induce tranquilization. The main treatment is as follows: asthenia, collapse, cold limbs, weak pulse, spleen deficiency, anorexia, lung deficiency, cough, asthma, body fluid deficiency, thirst, internal heat, asthenia, palpitation, insomnia, sexual impotence, and cold womb; heart failure, cardiogenic shock. Can be used for treating short breath, dyspnea, palpitation, amnesia, thirst, hyperhidrosis, anorexia, asthenia, acute and chronic diseases, and shock and collapse caused by blood loss. Tonify primordial qi, relieve depletion and promote fluid production, induce tranquilization. It is indicated for deficiency due to overstrain, poor appetite, lassitude, regurgitation, diarrhea, cough, dyspnea, spontaneous sweating, palpitation, amnesia, vertigo, headache, impotence, frequent micturition, diabetes, metrorrhagia, metrostaxis, chronic infantile convulsion, and deficiency of both qi and blood. 2. Treatment of cardiovascular system diseases: ginseng radix has therapeutic effect on hypertension, myocardial malnutrition, coronary arteriosclerosis, angina pectoris, etc., and can relieve various symptoms. Ginseng has a regulating effect on abnormal blood pressure, or it is thought that different doses may have different effects: the small dosage can increase blood pressure, and the large dosage can reduce blood pressure. 3. For gastric and hepatic diseases: for patients with chronic gastritis accompanied with achlorhydria or hyperchlorhydria, the increase of gastric sodium can be seen after taking ginseng, the symptom is relieved or disappeared, but the gastric secretion and the gastric acidity are not obviously affected. Ginseng has also been reported to cause the disappearance of stomachache, the enhancement of appetite, the normal defecation and the increase of total acidity of gastric juice in patients with chronic gastritis. For acute infectious hepatitis, under certain treatment conditions, taking ginseng has certain positive significance for preventing chronic hepatitis. 4. Treating diabetes: ginseng improves the general condition of diabetics without altering the hyperglycemic level. Or the radix Ginseng can reduce urine sugar of light diabetic patients by 40-50 mg%, and can maintain for more than 2 weeks after stopping administration; after the ginseng is taken by a moderate diabetic, although the effect of reducing the blood sugar is not obvious, most of the general conditions are improved, and symptoms such as thirst and the like disappear or are relieved; the dosage of insulin can be reduced after some patients take Ginseng radix. 5. For psychosis: ginseng has therapeutic effect on mental diseases of weakness type and weakness-depression type, no matter what the cause (schizophrenia, intoxication or psychosis caused by infectious disease, degenerative psychosis, etc.). It is also believed that oral administration of ginseng can only improve the general subjective symptoms of patients with organic neurological disorders, without an objective and significant therapeutic effect. 6. Treating neurasthenia: the ginseng has obvious excitation function on the nervous system, can improve the activity of the organism and reduce fatigue; has certain therapeutic effect on different types of neurasthenia patients, increases the weight of the patients, and eliminates or relieves symptoms of weakness, headache, insomnia and the like of the whole body. 7. Treating impotence: ginseng, in traditional Chinese medicine, is generally used as a tonic and can tonify primordial qi; the recent research proves that it has the function of enhancing the sexual gland function. The ginseng tincture has obvious curative effect on paralytic and premature ejaculation type impotence, but is ineffective on mental type; it also has therapeutic effect on cortical and spinal impotence caused by neurasthenia. 8. Ginseng also has effects of improving vision and enhancing dark adaptation. 9. Can be used together with other medicines for treating various diseases. Ginseng has a wide range of pharmacological actions. For example, in its effect on the central nervous system, ginseng has an excitatory effect on the central nervous system. Can enhance the excitation and inhibition process of the higher nerve activity of animals. It can also enhance the adaptability of organism to any non-specific stimulation, and reduce fatigue feeling (root, stem and leaf of Ginseng radix can prolong the swimming duration of mice).

Ginseng can regulate the balance between excitation and inhibition of central nervous system. The results of the research on the influence of the ginseng on the brain electrical activity of the animal show that: it has effects on both excitatory and inhibitory neural processes, but primarily enhances the excitatory processes of the cerebral cortex. By acting simultaneously on the inhibition process, the inhibition tends to be concentrated, resulting in accelerated and more complete differentiation. Ginseng radix has effects of regulating nerve function, and recovering nervous process caused by disorder due to stress. Ginsenoside Rb has central tranquilizing effect, and mixed saponins of Rb1, Rb2 and Rc have tranquilizing effect; rg has central excitation. The effects of ginsenoside on the center are small dose excitation and large dose inhibition. The 5g/kg intraperitoneal injection of the ginseng infusion can obviously reduce the spontaneous activity of mice. The Ginseng radix infusion can also be used for relieving convulsion due to cocaine, strychnine and pentamidine, and reducing convulsion death rate. It is reported that the crude neutral saponin of ginseng has the effects of tranquilizing and allaying excitement, relieving pain, relaxing muscles and lowering the temperature.

Ginseng has bidirectional and component-dependent effects on learning and memory. The Ginseng radix extract can prevent the learning ability of mice from decreasing due to stress. It is reported that the ginseng extract has antagonistic action on poor memory acquisition caused by anisodine and sodium pentobarbital, and can also improve memory consolidation disorder caused by cycloheximide and sodium nitrite and memory reproduction defect caused by 40% ethanol.

Ginseng radix can be used for preventing and treating acute and chronic altitude diseases due to anoxia. Under the high hypoxia condition with the altitude of 7000m, the change research of the rat brain cortex ultrastructure under the acute hypoxia condition shows that the ultrastructure of the brain cortex neuron is obviously protected from hypoxia damage; experiments also observe that the ginsenoside has obvious effect of inhibiting the utilization of endogenous glycogen. Meanwhile, the preparation can enhance tissue respiration, promote anaerobic glycolysis, improve productivity under an anaerobic condition and reduce oxygen consumption of an organism, so that neurons and a cardiovascular system can be protected to improve the tolerance to the anaerobic condition. The clinical verification proves that the panaxaside is effective in preventing and treating acute and chronic altitude diseases. Ginseng radix can improve oxygen uptake capacity of brain. Ginseng also has significant effects on cerebral blood flow and cerebral energy metabolism. The ginseng preparation can increase the glucose uptake of rabbit brain, reduce the ratio of lactic acid, pyruvic acid and lactic acid/pyruvic acid, and change the glucose utilization from anaerobic metabolism to aerobic metabolism. Ginseng also increases the amount of free inorganic phosphorus in the cerebral cortex by 25%. The ginsenoside can improve brain oxygen uptake. The total saponins of Ginseng radix and Ginseng radix have protective effect on cerebral ischemia/reperfusion injury. In a word, ginseng can make the animal brain more reasonably utilize the energy substance glucose, oxidize the energy and synthesize more ATP for activities such as learning and memory.

The ginseng plateau mixture has the capacity of resisting plateau anoxia. Acute pressure-reducing hypoxia experiments prove that the ginseng plateau mixture can obviously reduce the death rate of mice and improve the acute hypoxia resistance. The anti-anoxia mechanism of ginseng high-altitude mixture needs to be further researched, and may be related to the effects of improving blood circulation of heart, brain, lung and the like, improving myocardial metabolism, resisting thrombosis, dissolving thrombus, improving blood rheological property and adapting to original shape. The ginseng plateau mixture needs to be clinically applied to test the curative effect of resisting plateau anoxia.

The ginseng has the anti-fatigue effect, and human body experiments prove that: the mechanism of the anti-fatigue effect of ginseng is probably related to the functions of increasing blood fat and promoting protein and RNA synthesis. Studies show that ginsenoside Rg1 has remarkable antifatigue effect, and neutral saponin (Rb1, Rb2, Rc, etc.) has no antifatigue effect. The lipophilic component remained after separating ginsenoside can also increase spontaneous movement of mouse, and has antifatigue effect. Ginseng radix has the advantages of economic availability of glycogen and high energy phosphate, preventing accumulation of lactic acid and pyruvic acid, and promoting metabolism. Ginseng also prevents depletion of glycogen and cholesterol in adrenal glands in tissues caused by long-term exercise in rats. The research shows that: ginseng radix has central cholinergic activity and catecholamine activity, and has effects of enhancing choline system function, increasing Ach synthesis and release, and increasing central M-choline receptor density.

Experiments prove that: ginseng radix can promote protein synthesis, RNA synthesis and DNA synthesis. The function of ginseng in facilitating memory may be mainly related to the synthesis of nucleic acid and protein in brain. Rg1 can increase the protein content in brain, while Rb1 has no effect. It is reported that ginsenosides can significantly increase the RNA content in mouse brain. The ginsenosides such as panaxadiol saponins and panaxatriol saponins have no obvious change on the normal level of gamma-aminobutyric acid in the brain of mice, but have obvious effect on resisting the reduction of the level of gamma-aminobutyric acid in the brain caused by isoniazid.

Ginseng radix can improve body adaptability. Ginseng radix can change the reactivity of organism, and has similar effects as radix Acanthopanacis Senticosi and fructus Schisandrae Bicoloris, etc., i.e. it can enhance the reaction ability of organism to various harmful stimuli and enhance the adaptability of organism. As a regulator of body functions, ginsenosides such as saponin of stem and leaf and root of Panax ginseng have protective effects on stress reactions caused by various stimuli such as physical (cold, overheating, severe exercise, radiation), biological (alloserum, bacteria, transplanted tumor), chemical (poison, narcotic, hormone, anticancer drug, etc.), and can restore the normal function of disorder, and are called as an adaptive substance (a substance for enhancing the nonspecific resistance of human body). When the dog is in a critical state due to massive blood loss or suffocation, the ginseng preparation is injected immediately, so that the blood pressure reduced to a very low level is stabilized and increased, the survival time of the animal is prolonged, and the animal is promoted to recover the health. Ginseng can prevent hypertrophy of adrenal gland caused by ACTH and atrophy caused by prednisone; preventing hyperthyroidism caused by thyroxine and hypofunction caused by 6-methylthiouracil; can reduce the hyperglycemia of the dieting and can also increase the hypoglycemia caused by insulin; leukocytosis caused by subcutaneous injection of cow milk can be reduced, and leukopenia caused by dysentery endotoxin can be increased. The rabbit taking Ginseng radix for a long time can prevent fever reaction caused by intravenous vaccine. Has certain good effects on symptoms and anaphylactic shock caused by deficiency of vitamin B1 and vitamin B2. But no significant anti-adrenergic or anti-histamine effects. The action principle of the ginseng can be related to the reaction of the ginseng to the body in a stress process, particularly to the influence of the nerve-pituitary-adrenal cortex system. The report shows that the saponin abdominal cavity injection of ginseng stem and leaf can obviously reduce the death rate of mice under the conditions of high temperature (46 ℃) and low temperature (minus 9 ℃), and has the functions of high temperature resistance and low temperature resistance; compared with ginsenoside, the two have similar effects. The administration of saponin from stem and leaf by intragastric administration is continued for 3 days, and can protect scald shock. Because ginseng can strengthen the resistance of the body to harmful factors, it is of great significance in the treatment of many infectious diseases.

It is used for treating cardiovascular diseases. 1. Effects on cardiac function: ginseng has the effects of stimulating first and then inhibiting, stimulating a small amount and inhibiting a large amount of the hearts of various animals. It has similar action to cardiac glycoside and can improve myocardial contraction. Large doses attenuate contractility and slow down heart rate. Experiments show that: the alcohol extract and water extract of Ginseng radix Rubri can be used for treating frog heart in vitro to enhance its contraction, and stopping in contraction phase; for dogs, rabbits and cats with heart-positioning, it can also enhance their contraction and slow down their heart rate. These effects are mainly due to direct stimulation of the myocardium. For acute circulatory failure (slow heart rate and weak heart) caused by massive blood loss of animals, the ginseng can increase the heartbeat amplitude abnormally and increase the heart rate remarkably. Ginsenoside has strong effect in resisting rat arrhythmia induced by barium chloride, and can be used for correcting tachycardia and restoring heart rhythm to normal level. It has been reported that ginsenosides can be used against experimental arrhythmia caused by adrenalin. Ginsenoside has bidirectional regulation effect on cAMP and cGMP content in myocardial cells, so maintaining balance of cAMP and cGMP is also a factor for resisting heart rhythm disorder under stress state. The ginseng stem leaf total saponin has protective effect on rabbit experimental sinus node function damage. 2. Effect on myocardium: ginseng radix has effect in protecting myocardium. Ginsenoside can reduce lactic acid content in brain and myocardium of mouse under severe anoxia, recover myocardial cAMP/cGMP ratio during anoxia, protect myocardial capillary endothelial cell, and relieve mitochondrial injury. The saponin extracted from the stem, leaf, head, fruit and main root of ginseng has obvious myocardial protecting effect on rat myocardial necrosis caused by isopropyl adrenaline, and may be used in relieving disease damage, especially ginseng fruit saponin. Saponins in different parts of Ginseng radix have similar effects with propranolol. The total ginsenoside can promote DNA synthesis of myocardial cells of suckling mice cultured in vitro, and has certain protective effect on myocardial cells cultured under the conditions of sugar deficiency and oxygen deficiency injury. The research suggests that the mechanism of resisting myocardial ischemia and reperfusion injury of the ginseng total saponin is to promote the generation and release of prostaglandin, inhibit the generation of thromboxane A2 and protect myocardial cells by resisting oxygen free radicals and lipid peroxidation. 3. Effect on blood vessels: the action of ginseng on blood vessels is generally considered as a vasodilator, but it has also been reported to contract at a small dose, expand at a large dose or contract first and then expand. The action of ginseng on blood vessels is different depending on the kind of blood vessel or the condition of the body. Ginseng radix has effect in constricting blood vessels of rabbit ear and hind limb of rat. But has the function of dilating coronary artery, cerebral vessels and fundus blood vessels of the whole animal. Intravenous total saponins reduce hind limb vascular and cerebrovascular resistance in dogs, but increase renal vascular resistance in rats. Ginsenoside Rg1 and Re also have dilating effect on canine blood vessel, and the effect is 1/20 and 1/50 of papaverine, Rc and Rb2 have weak effect, and Rb1 has no effect. The research on the effective components and action mechanism of the ginseng influencing the function of blood vessels shows that: the vasodilatory action of ginsenosides Rb1 and R0 is non-selective, while Rg1 is only selective against Ca + + induced vasoconstriction, the mechanism of action of which remains to be studied further. It is believed that the different regulation of blood vessels by ginseng under different classes and different physiological states may be the reason why ginseng regulates blood pressure bidirectionally. 4. Effect on blood pressure: most data show that: in normal or high blood pressure state of animals, ginseng has the function of lowering blood pressure, but also has been reported to increase blood pressure. The ginseng can increase the blood pressure of anesthetized animals with small dose and reduce the blood pressure with large dose. The treatment amount has no obvious influence on the blood pressure of patients. The pressure-raising action of ginseng may be related to the reduction of the volume of kidney and spleen and the contraction of visceral blood vessels. Whereas the reduced pressure is due to histamine release. The anaesthetized dog has quick tolerance to the blood pressure lowering effect of the ginseng. Ginsenoside Rb1, Rb2, Rc, Rd, Re, and Rf has two-way effect of blood pressure decrease after microlitre, with Rg1 being strongest and Rb1 being high dose for increasing blood pressure. But its effect on blood pressure is not affected by atropine, diphenhydramine, oxazoline and propranolol. Intravenous injection of 40 mg/kg of the ginseng ether extract can slow down the heart rate of dogs lightly anesthetized with halothane and lower the central venous pressure. It is worth noting that: intravenous injection of Ginseng radix extract can recover cats with stopped respiration, lowered blood pressure, and completely disappeared reflex from dying state. The mechanism of the blood pressure lowering effect of ginseng is considered as follows: ginseng radix has choline-like effect; the transient hypotensive effect of the red ginseng ether extract is related to histamine release, and the persistent hypotensive effect is caused by other reasons; the ginseng can reduce Ca + + in blood vessels, the blood pressure reducing effect is the result of the effect of the ginseng on vascular smooth muscle, and the ginsenoside Rb1 has the effect of reducing blood pressure permanently; because the blood pressure lowering effect of ginseng can be eliminated after the brain of an animal is cut off or a ganglion blocking agent is used, the blood pressure lowering effect of ginseng can possibly involve the central nerve and a reflex mechanism. 5. The function of anoxia endurance: the ginseng or the extract thereof can obviously improve the hypoxia tolerance of animals, slow the oxygen consumption speed, prolong the survival time and prolong the contraction time of atria under the hypoxia condition. Ginseng radix Rubri has better ability of improving anoxia resistance than sun-dried Ginseng radix. The mechanism of action of ginseng in improving the hypoxia tolerance of the organism is probably related to factors such as reducing the oxygen consumption of cardiac muscle, increasing the blood flow of coronary artery, increasing the content of 2,3-DPG in red blood cells, regulating the cyclic nucleotide metabolism and the glycometabolism of cardiac muscle and the like. Experiments prove that: the 10 percent ginseng extract is injected into the abdominal cavity of a mouse, so that the normal pressure hypoxia resistance of the mouse can be obviously improved, and the sodium nitrite poisoning hypoxia resistance of the mouse can also be improved. The panaxoside can obviously reduce the oxygen consumption of animals, enhance the tolerance of mice under the conditions of low pressure and normal pressure hypoxia, and obviously prolong the survival time of the mice when the mice have cerebral circulation disturbance hypoxia and tissue toxicity hypoxia, which is consistent with the action of ginseng roots. Ginseng also has the effect of reducing myocardial oxygen consumption or increasing coronary blood flow, which is also related to the improvement of anoxia resistance of the body. The ginsenoside reduces the content of lactic acid in tissues and the content of cAMP and cGMP in cardiac muscle when mice are in hypoxia, and the ratio of cAMP/cGMP is close to normal. The total ginsenoside can prevent anaerobic glycolysis of anoxia and glucose deficiency myocardial cells, promote glycogen synthesis, and protect anoxia and glucose deficiency myocardial cells. 6. Effects on hematopoietic function: ginseng radix or its extract has effects of protecting and stimulating hemopoietic function of bone marrow, and increasing erythrocyte count, leukocyte count and hemoglobin amount of normal and anemia animals. It can also increase the number of red blood cells, hemoglobin and platelets in anemia patients. The effect of ginseng on increasing the number of peripheral blood cells is more pronounced when peripheral blood cells are reduced or bone marrow is inhibited. Ginseng stimulates hematopoietic function by increasing synthesis of bone marrow DNA, RNA, proteins and lipids, promoting mitosis of bone marrow cells. 7. Effects on platelet function: ginseng radix has effect in inhibiting platelet aggregation. 1.5g of red ginseng powder is orally taken to healthy adults on an empty stomach, blood is drawn 1 and 3 hours after the oral administration to measure platelet aggregation, and compared with the result before the oral administration, the red ginseng powder has a remarkable inhibiting effect on platelet aggregation induced by arachidonic acid, ADP, thrombin, epinephrine and the like, and the generation of platelet malondialdehyde induced by the arachidonic acid and the thrombin is also inhibited. Comparing the inhibitory effect of 70% methanol extracts of red ginseng and white ginseng on rabbit platelet aggregation in vitro, the red ginseng extract was found to have a stronger effect than the white ginseng extract. The research on the platelet aggregation inhibiting mechanism of ginseng shows that the ginsenoside can excite adenylate cyclase on platelet membrane and inhibit the activity of phosphodiesterase so as to obviously increase the cAMP level in platelets. Since the aging curves for ginsenoside inhibiting ADP, arachidonic acid, and collagen-induced platelet aggregation in vivo are consistent with the aging curves for elevated platelet cAMP levels, ginsenoside elevated platelet cAMP levels are likely one of the mechanisms by which ginsenoside inhibits platelet aggregation in rabbits. Ginsenoside significantly increased the cAMP content in platelets, but did not affect the cGMP content. Experiments indicate that ginseng has direct effect on platelet epoxidase or TXA2 synthetase, and the function of ginseng for inhibiting platelet is related to PG metabolism. The research result shows that: the ginseng or ginsenoside has inhibitory effect on blood platelet. The action mechanism of the compound is probably related to the functions of blocking PG metabolism, increasing cAMP content in platelets, Ca + + antagonism and the like. 8. Blood fat reducing and anti-atherosclerosis effects: the ginseng, especially ginsenoside Rb2, has effects of improving blood lipid, reducing blood cholesterol and triglyceride, increasing serum high density lipoprotein cholesterol, and reducing arteriosclerosis index, and can be used for treating hyperlipidemia, thrombosis and arteriosclerosis. Ginsenoside has effect in promoting lipid metabolism of normal animal, promoting biosynthesis, decomposition, transformation and excretion of cholesterol and blood lipoprotein, and reducing blood cholesterol, and can be used for treating animal hypercholesterolemia. Ginsenoside and panaxan have blood lipid reducing effect on rat with hyperlipidemia. Ginseng radix Rubri powder, ginsenoside Rb2, Rc, Rg1, and Rb1, especially Rb2 can increase blood high density lipoprotein cholesterol, and has good effect in resisting atherosclerosis. Ginsenoside also has effect in preventing experimental atherosclerosis. Ginsenoside Rb2 can reduce total cholesterol, free cholesterol and low density lipoprotein cholesterol, increase high density lipoprotein cholesterol, improve arteriosclerosis index, and has effect after once intraperitoneal injection, and multiple administration effect is more remarkable. Studies have shown that ginsenoside Rb2 has effects of dissimilatorizing cholesterol and promoting excretion, and triglyceride promotes its transfer into adipose tissue. Ginseng radix has blood lipid reducing effect for both healthy people and hyperlipemia patients. After 5 healthy people and 6 patients with hyperlipidemia take the red ginseng powder orally for 1 week, serum cholesterol is not reduced obviously, but serum high-density lipoprotein cholesterol is increased obviously, and the atherosclerosis index is reduced obviously. It can also be used for treating hypertriglyceridemia. 9. Ginseng has direct effects on cardiac muscle and blood vessels, and is generally excitatory at low doses and inhibitory at high doses. The 10% ginseng extract is used for gastric lavage of cats (or rabbits) at a concentration of 1 ml/kg, and has certain effect of improving myocardial weakness. There was a considerable recovery during the rewarming period. It also has antiallergic shock resisting and heart tonifying effects. Ginseng radix has effect in inhibiting ATPase activity of rat myocardial cell membrane. Anti-shock effect: ginseng can relieve anaphylactic shock induced by guinea pig serum, and prolong its survival time. For the scald shock mice, the death can be obviously delayed. It can be used for treating dogs in hemorrhagic and asphyxia crisis states, and promoting recovery of normal activity. For animals with hemorrhagic acute circulatory failure, ginseng can significantly increase the amplitude of heart beat and the heart rate. In heart failure, the cardiotonic effect is more remarkable. The administration of the ginsenoside in advance can prolong the survival time of hemorrhagic shock dog, prevent damage to myomembrane, nuclear membrane and mitochondria of myocardial cell of hemorrhagic shock, and protect heart, brain, kidney and liver of shock animal. The ginsenoside and ginsenosides have effects of protecting heart, liver and lung tissue in hemorrhagic shock. Ginseng radix extract (prepared by percolating Ginseng radix Rubri, radix Ginseng alba, and 20% ethanol at a ratio of 1: 1) has insulin shock resisting effect, and Ginseng radix total saponin has insulin shock promoting effect. The Shenmai injection (Ginseng radix, radix Ophiopogonis) has good effect in treating experimental endotoxic shock of mice and rats, and can relieve diarrhea and fever symptoms and improve functions of peripheral hemogram and reticuloendothelial system. Suggesting that it is a good activator of reticuloendothelial system function. Effects on the liver: ginseng radix can increase enzyme activity of liver metabolism substances, enhance liver detoxication ability, and enhance tolerance of organism to various chemical substances. Experiments show that: ginseng radix can increase activity of alcohol dehydrogenase in liver, shorten anesthesia time of ethanol for rabbit and dog, and rapidly reduce ethanol level in rabbit blood. It is reported that ginseng can enhance the detoxifying function of liver and also has the effect of resisting liver injury. Ginsenoside has effect in relieving liver injury transaminase blood disease caused by carbon tetrachloride. It has also been reported that ginsenoside can reduce the increase of rabbit serum glutamic-oxaloacetic transaminase activity caused by carbon tetrachloride, and can also reduce the change of rabbit liver tissue caused by other toxicants, such as thioacetamide. The research results of the anti-hepatotoxic effect and certain structure-effect relations of the ginsenoside show that the red ginseng has stronger anti-hepatotoxic activity than the white ginseng. The ginseng has obvious detoxification effect on ethanol, can shorten the duration of ethanol anesthesia and accelerate the recovery time, can reduce the contents of GOT, GPT, ALP, bilirubin and the like in serum, can increase the activities of alcohol dehydrogenase and aldehyde dehydrogenase related to ethanol metabolism, can quickly discharge toxic substance acetaldehyde generated by ethanol metabolism out of the body, and can effectively protect the liver suffering from ethanol poisoning because excessive hydrogen participates in saponin synthesis. Effects on endocrine system. Effects on pituitary, adrenal cortex: ginseng is believed to have no corticoid effect itself, but it has also been proposed to excite the adrenal cortex. The research shows that: ginseng radix has stimulating effect on pituitary-adrenal cortex system, and its effective component is ginsenoside. Various ginsenosides have different stimulating effects due to their different chemical structures. The action site of ginsenoside is above the level of pituitary, and ginsenoside does not directly act on the biochemical process of ACTH secretion from anterior pituitary, and the action of ginsenoside must be realized by cAMP through a second messenger. The ginseng can make the adrenal of rats with normal and excised adrenal gland be hypertrophied; reducing the content of 17-ketosteroid in the guinea pig urine; the eosinophilia of rats is increased, the vitamin C and the cholesterol in the adrenal cortex are reduced, and the ACTH in the urine is increased. Under the stress conditions of low pressure and anoxia, Ginseng radix can prevent the content of vitamin C in rat adrenal gland from decreasing. Ginseng improves the ability of mice to withstand high temperatures and low temperatures, but this effect disappears after removal of the adrenal gland. The ginsenoside Rb is the strongest among various ginsenosides capable of increasing the secretion activity of adrenocortical hormone. The alpha-receptor antagonist phentolamine, the beta-receptor blocker propranolol, the nerve blocker hexamethyldiamine and the hypnotic pentobarbital sodium can not be antagonized by intraperitoneal injection, and the level of corticosterone in blood plasma is increased after 7 mg/100 g of ginsenoside is injected into the abdominal cavity of a rat for 30 minutes. Ginsenoside stimulates adrenal cortex to increase blood plasma corticosterone level. After long-term administration of ginsenoside, the weight of adrenal gland of rat can be increased. Ginsenoside mainly acts on adrenal cortex to make cortex hyperplasia thick. As cortical hormone secretion increases, the weight of the adrenal gland increases and the thymus also atrophies. It has been reported that the mechanism of ginsenoside stimulating the increase of secretion of adrenocortical hormone is: ginsenoside stimulates adrenal cortex function by releasing pituitary ACTH, which must in turn be stimulated by cAMP through a second messenger. Experiments prove that: the increase in the concentration of cAMP in the adrenal gland is related to the dosage of ginsenoside. Injecting ginsenoside into abdominal cavity of rat, wherein when dosage is above 5 mg/kg, the concentration of cAMP in adrenal gland of animal is obviously higher than that of control group. At the same time, the plasma concentration of 17-hydroxycorticosteroid in rats is also significantly increased, while the adrenocorticoid tends to decrease, possibly as a result of the release of corticoid into the blood. It was further demonstrated that the plasma changes of ACTH paralleled those of corticosterone after intraperitoneal injection of ginsenoside into rats. It has been reported that the action site of ginsenoside is at or above the pituitary gland. In hypophysectomized rats, the effects of ginseng on ACTH and intraadrenal cAMP levels in the blood were absent. Panaxadiol and ginsenoside Rd have the effect of increasing adrenal cAMP level in rats, but ginsenoside Rb2 and panaxatriol are ineffective, and the effect of Rd and panaxadiol on adrenal cAMP content can be relieved by prolapsed. Ginseng has an antidiuretic effect on mice and is markedly attenuated after castration, removal of the pituitary or adrenal gland, which is thought to act on the posterior pituitary pathway. The antidiuretic action of the 20% alcohol extract of the ginseng root, stem and leaf has approximate direct proportion relation with the dosage, the antidiuretic effect of the animal is still not lost by removing the pituitary or pineal or anaesthetizing the animal by using pentobarbital sodium, but can be antagonized by spironolactone, and the action is considered to be caused by promoting the adrenal cortex to secrete mineralocorticoid hormone; it was also found that there was a significant increase in serum potassium before the antidiuretic effect was observed, presumably an increase in serum potassium as a result of stimulation of aldosterone secretion. Effects on substance metabolism: 1. experiments prove that: ginseng radix has effects of inhibiting hyperglycemia and hyperglycemia caused by injection of epinephrine and hypertonic glucose. It has effects of controlling blood sugar level of male rat with alloxan diabetes, but can not prevent morbidity and mortality. It is also effective in treating diabetes caused by alloxan in mice. Has certain inhibiting effect on the experimental hyperglycemia of the dogs, but can not completely correct the metabolic disturbance of the dogs. It has also been reported that different saponin monomers of ginseng are capable of potentiating the effects of insulin on glucose metabolism against the effects of epinephrine, ACTH and glucagon. Experiments show that: the Ginseng radix extract, Ginseng radix polysaccharide, Ginseng radix polypeptide, Ginseng radix stem and leaf polysaccharide, and Ginseng radix non-saponin part all have blood sugar lowering effect, and can be used for treating diabetes. The Ginseng radix extract has effects of lowering blood sugar level, reducing ketone body, and promoting sugar absorption for alloxan diabetes. 30 mg/kg and 60 mg/kg of ginseng polypeptide can be injected into rabbit vein, and can significantly reduce blood sugar and liver glycogen content. The ginseng stem and leaf polysaccharide 50mg/kg and100 mg/kg can be injected into abdominal cavity or vein of mouse, and can obviously resist the hypertension of epinephrine and alloxan. The ginseng polysaccharide 50 mg/kg-200 mg/kg is injected into abdominal cavity or subcutaneous cavity of mouse to reduce blood sugar and liver glycogen content, and adrenal gland resection does not affect its effect. The ginseng polypeptide 50mg/kg, 10 mg/kg and 200 mg/kg can be administered to rats by intravenous injection or subcutaneous administration for several times, and can reduce normal blood sugar and liver glycogen. Simultaneously, the composition has inhibition effect on hyperglycemia caused by adrenalin, alloxan and glucose, and can enhance the decomposition of hepatic glycogen by adrenalin. The research on the blood sugar reducing mechanism of ginseng finds that the blood sugar reducing effect of ginseng polypeptide is mainly caused by the fact that the activity of succinate dehydrogenase and CCD is stimulated to enhance the aerobic oxidation effect of sugar besides the effect of promoting glycogenolysis or inhibiting lactic acid from synthesizing liver glycogen. The panaxan can also increase pyruvate content and inhibit lactate dehydrogenase activity to reduce lactate. The ginseng polysaccharides can also enhance the activity of succinate dehydrogenase and cytochrome oxidase. The ginseng can stimulate free islet of mice to release insulin, can obviously increase the synthesis amount of the insulin, and has no obvious influence on the blood sugar level, the insulin level and the insulin-glucose level of the diabetic mice caused by the streptourease. The ginsenoside can stimulate insulin release from isolated rat islets and promote insulin release caused by glucose. Administering Ginseng radix Rubri powder 3-6g or saponin component once for normal people and diabetes patients, and reducing blood sugar; increased gastrin in the blood; corticosterone values decreased in diabetic patients and increased in normal persons; catecholamine value has a tendency to decrease. Catecholamine can activate adenylate cyclase on cell membrane, increase cAMP, promote gluconeogenesis, and Ginseng radix Rubri can decrease catecholamine content, thereby limiting gluconeogenesis and regulating sugar metabolism. Ginseng radix has two-way regulation effect on glucose metabolism, and can reduce blood sugar of hyperglycemia and increase blood sugar of hypoglycemia caused by insulin. In addition, ginseng also has the function of strengthening the body's resistance to harmful factors.

Prepared rehmannia root, original name: rehmannia, also known as: radix rehmanniae and radix rehmanniae Preparata. Latin name: rehmannia glutinosa (Gaetn.) libosh.ex fisch.et mey. scrophulariaceae, Rehmannia, dense-covered white, multicellular, long, velvety and glandular hairs. The rhizome is used as a medicine. The prepared rehmannia root, a traditional Chinese medicine, is a processed product of rehmannia root, has black and glossy surface, soft and flexible quality, sweet taste and mild nature, and has the effects of nourishing yin and supplementing blood, replenishing vital essence and replenishing marrow and the like. Can be used for treating deficiency of liver-yin and kidney-yin, soreness of waist and knees, hectic fever, night sweat, nocturnal emission, internal heat, diabetes, blood deficiency, sallow complexion, cardiopalmus, menoxenia, giddiness, tinnitus, and premature gray hair. The dried rehmannia root is prepared rehmannia root which is fumigated to be black and moist, is irregular block-shaped and has different sizes, the inside and the outside are painted black, and the product has luster and the outer surface is wrinkled and uneven. Has moist cross section, bright grease-like block in the center, high viscosity, soft texture, slight smell, and sweet taste. It is better to have a big, soft and moist root tuber, and black and glossy interior and exterior through nine times of steaming and sun drying.

The nature, flavor and meridian tropism. Sweet in taste; and (4) temperature. Liver diseases entering; the kidney channel. The efficacy is mainly as follows: enriching blood and moistening; replenishing vital essence and marrow; for sallow complexion due to blood deficiency; dizziness and palpitation; menoxenia; collapse is not stopped; deficiency of liver-kidney yin; tidal fever and night sweats; spermatorrhea and impotence; infertility; menoxenia; metrorrhagia and metrostaxis and bleeding; soreness and weakness of waist and knees; tinnitus and deafness; dizziness and blurred vision; the beard and hair are early white; quenching thirst; constipation; dyspnea and tachypnea due to kidney deficiency.

Rehmannia has a wide range of pharmacological actions. The comparison of the pharmacological effects of rehmanniae radix Preparata and rehmanniae radix upon the intravascular thrombotic syndrome shows that: the crude radix rehmanniae Preparata of pig gallbladder shape produced in China can strongly inhibit hemorrhagic necrosis of liver and simple necrosis. The antithrombin effect of rehmanniae radix preparata is weak. The activation of the fibrinolytic system was studied by the activation of plasminogen using the fibrin plate method, and it was found that rehmanniae radix had the activation but rehmanniae radix did not. It is also reported that the hemostatic action of rehmannia root before and after charring is not enhanced. The hemostatic effect of the water decoction of rehmannia root, prepared rehmannia root, rehmannia root charcoal and prepared rehmannia root charcoal is analyzed, and the result has no significant difference. The experiment shows that: the Huaiqing prepared rehmannia root decoction can relieve the weight loss of hyperthyroidism type yin-deficiency rats after being infused with water, obviously reduces the water intake and urine volume in 24 hours, obviously improves the concentration levels of T3 and T4 in blood plasma (namely, reduces T3 and increases T4) and obviously increases the level of AD (aldosterone in blood plasma). Proved that the medicine has stronger effect of nourishing yin. It is thought that the yin-nourishing and kidney-tonifying effects of rehmanniae radix Preparata are related to improving AD level in vivo.

The prepared rehmannia root decoction can obviously increase the activity of glutathione peroxidase (GSH-Px) in animal serum when being irrigated to a mouse; reduce the content of lipid peroxide in serum. Can increase the activity of superoxide dismutase (SOD) in blood to a certain extent, but has no statistical significance. Has no influence on the catalase activity in blood. Radix rehmanniae Preparata has certain effect on protein metabolism of rat viscera. Radix rehmanniae Preparata orally has the effect of inhibiting mitosis of epithelial cells. Experiments show that: the Liuwei Dihuang decoction taking prepared rehmannia root as a main drug can increase the weight of a normal mouse, prolong the swimming time and enhance the physical strength, can reduce the induction rate of mouse anterior squamous cell carcinoma caused by N-nitrososarcosine ethyl ester, enables the spleen lymph nodule of an animal receiving a chemical carcinogen to grow and activate the proliferation of a center of hair growth, can enhance the phagocytic activity of a mononuclear macrophagy system at the initial stage of inoculation of a transplanted tumor, improves the proportion of albumin and globulin in the blood of the tumor-bearing animal, and can similarly prolong the survival time of the tumor-bearing animal. Firstly, the prepared rehmannia root has the effect of delaying the aging of mice: the 20% water decoction of radix rehmanniae Preparata is administered by intragastric administration for 45 days, and blood is sacrificed and blood is taken out, and activity of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and content of Lipid Peroxide (LPO) are determined. As a result, rehmanniae radix Preparata can enhance GSH-Px activity and reduce LPO content in serum, which is P <0.05 and P <0.01, respectively, compared with control group. The effect on hyperthyroidism type yin deficiency rats is as follows: administration of triiodothyronine (T3) to rats resulted in a yin deficiency model and 3ml (70% concentration) of yellow water decoction of rehmannia glutinosa for 6 days, after which each data was measured. Results compared to the administered group, the normal control group had no matter signs, weight change, 24-hour water intake and urine intake, and related hormones: there were no significant differences in plasma concentrations of T3, thyroxine (T4) and Aldosterone (AD) (P > 0.05). However, after the model is made, the yin-deficiency group has very obvious changes compared with the normal group, such as good excitement, weight loss, increase of 24-hour drinking amount and drinking amount, increase of T3 concentration, decrease of T4 and AD concentration and the like. The symptoms and indexes of the yin-deficiency rats are obviously improved after the radix rehmanniae preparata is fed, and the symptoms and indexes are normal (compared with the yin-deficiency group). For example, compared with the group with yin deficiency and radix rehmanniae preparata, the AD concentration in blood plasma is obviously increased (P <0.05) and the urine volume is obviously reduced (P <0.01) in 24 hours, which indicates that the radix rehmanniae preparata has the function of nourishing kidney yin and is related to the improvement of the AD level in vivo. The concentrations of T3 and T4 in the plasma of the yin-deficiency administration group are obviously improved (the concentration of T3 is reduced, and the concentration of T4 is increased), and the concentration is normal. The prepared rehmannia root can improve the symptom of yin deficiency through the systemic regulation effect and can regulate the abnormal thyroxine state. In addition, compared with the normal group, the yin deficiency administration group still significantly did not reach the normal level in terms of weight change, 24-hour water intake, plasma T3, T4 concentration and the like, and all were significantly different (P < 0.01). This indicates that the index of the group administered with yin deficiency was significantly improved and tended to be normal, but not completely restored to normal, as compared with the group administered with yin deficiency. Indicating that the action of prepared rehmannia root is still limited. Experimental observation of the blood replenishing effect of prepared rehmannia root: preparing raw and cooked rehmannia glutinosa Libosch into a raw and cooked rehmannia glutinosa Libosch powder 1: the decoction 1 is administered by intragastric administration to mice to observe the effects on hemorrhagic anemia, hematopoietic stem cells and CFU-E production of myeloid erythroid progenitor cells. As a result: the fresh and cooked rehmannia has obvious effect on blood loss mice, after 8 days of administration, the prepared rehmannia root group recovers quickly and basically, and the P values of RBC and Hb counts are 0.001 and 0.01 respectively compared with the P values of a control group. Raw and prepared rehmannia root also has certain proliferation and differentiation effects on hematopoietic stem cells, and similarly, the blood enriching effect of rehmannia root is related to the generation of the hematopoietic stem cells for promoting blood cells. Production of hematopoietic cells from mouse bone marrow erythroid: the number of erythroid colony in normal saline group is 52.4 + -18.41, and the number of radix rehmanniae and radix rehmanniae Preparata groups is 60.2 + -19.44 and 125 + -20.45 respectively (P < 0.05). It is suggested that the blood-enriching action of rehmannia root is closely related to the hematopoietic system of bone marrow. The influence on the hemostasis effect after the rehmannia is fried to charcoal: rehmanniae radix, charred radix rehmanniae, radix rehmanniae Preparata, and charred radix rehmanniae Preparata are prepared into 100g crude drug or 33g charred drug per 100ml decoction respectively. Each sample was gavaged to mice at a dose of 0.8ml/20g, and blood was taken from the inner canthus of the left eye, retrobulbar venous plexus, and blood clotting times were counted and compared to saline group. The results have no significant difference (P >0.05), and indicate that the rehmannia root does not need to be processed for hemostasis.

Epimedium brevicornum Maxim.) perennial herbs. Epimedium herb is pungent and sweet in flavor, warm in nature, entering liver and kidney meridians, light in body weight and staminate in qi, and can ascend and descend. Has effects in invigorating kidney, supporting yang, expelling pathogenic wind, removing dampness, and strengthening bones and muscles. Can be used for treating kidney essence deficiency, sexual impotence, spermatorrhea, premature ejaculation, cold sperm, infertility, pollakisuria, cough, asthma, soreness of waist and knees, spasm of muscles and bones, rheumatalgia, numbness, spasm, hemiplegia, limb numbness, climacteric hypertension, dribbling, asthma, etc. Herba Epimedii has wide pharmacological action. It has effects in improving secretion of sexual hormone, improving sexual desire, supporting yang, and improving sexual function. The medicinal materials have the effects of strengthening essence and strengthening body. Can also be used for treating amnesia. Effects on sexual function: herba Epimedii can enhance secretion function of endocrine system such as hypothalamus-pituitary gland-gonad axis, adrenal cortex axis, and thymus axis, and can significantly increase weight of anterior pituitary, ovary, and uterus of rat. The inducing action is to stimulate the sensory nerve to excite the sexual desire indirectly after the seminal vesicle is full of the seminal vesicle, and the efficacy of the inducing action is superior to that of the hippocampus. The anti-aging effect is as follows: can obviously recover the functions of a D galactose aging model and the T and B lymphocyte proliferation reaction of male mice, can obviously improve the activity of the total SOD of the liver of the mice, reduce the formation of lipid peroxide in liver tissues, and reduce the formation of lipofuscin in heart and liver tissues and the like. Effects on the immune system: has bidirectional regulating effect on organism immunity, can increase spleen weight, and promote antigen-activated lymphocyte proliferation. Effect on the cardiovascular system: has obvious effect of increasing coronary artery flow of isolated rabbit heart, reducing resistance and lowering blood pressure caused by peripheral blood vessel dilation. The functions of relieving cough, eliminating phlegm and relieving asthma: the cough induction method by using sulfur dioxide in mice proves that the extract has the effect of relieving cough and is central. Also has protective effect on asthma. Increase in mouse sex organ volume and blood testosterone levels: after the epimedium extract or decoction is given to the male mice, the weight of prostate, seminal vesicle and levator ani is increased, the content of testosterone in plasma is increased, and the testosterone tissue proliferation and the testosterone secretion are increased. Icariine can promote the basal secretion of testosterone from testis interstitial cells and the generation of cAMP. After female rats are decocted with epimedium, the weight of anterior pituitary, ovary and uterus is obviously increased, and the specific binding force of the ovary HCG/LH receptor is obviously improved. Influence on sexual function pharmacological effects: because of its qi-tonifying and heart-calming effects, epimedium is often used to relieve stress and fatigue. Chinese folk physicians often add herba Epimedii (100g-200g, 10 times clinical dose) and cuttlefish decoction in red wine to treat fatigue and weakness due to overload. The basic principle that epimedium can treat fatigue and absentmindedness is to supply enough blood to the brain. In addition, epimedium is one of the components usually contained in spring medicinal liquor, and the usage of epimedium leaf decoction as a spring medicine is long-standing. Increase antibody production: the injection of total flavone of herba Epimedii, polysaccharide of herba Epimedii and icariine can increase antibody level of Sheep Red Blood Cell (SRBC) and hemolysin or spleen antibody production of immunized mouse. Herba epimedii regulates the propagation of lymphocytes: mice are respectively injected with epimedium total flavone or epimedium polysaccharide subcutaneously, and the injection is taken once a day and is continuously administrated for seven days, so that the lymphocyte transformation can be obviously promoted. The epimedium polysaccharide can increase the stimulation of mouse lymphocyte to Phytohemagglutinin (PHA). The 50% epimedium ethanol extract can obviously inhibit mouse lymphocyte proliferation reaction induced by mitogen. Its inhibitory response increases with increasing drug concentration. By injecting epimedium polysaccharide subcutaneously into mice with excessive doses of cyclo-Lin amidol, the production of Ts cells induced by Lin amidol with excessive doses can be promoted, and the inhibition on the generation of receptor mouse antibodies can be enhanced. In contrast, when mice immunized with the loop Lin amide super-aptamer were injected subcutaneously with icariin, Ts cells were attenuated and production of mouse antibody was increased. The mouse gavage epimedium decoction for causing yang deficiency model to prednisolone acetate can obviously increase the 3H-TdR incorporation and transformation of lymphocytes, and the titer of erythrocyte hemolysin and hemagglutination antibody. The epimedium decoction for gastric perfusion of mouse with low immune function caused by lymphocyte serum can restore the number of splenic lymphocytes and splenic plaque forming cell reaction of mouse with low immune function to normal level. Improving/restoring phagocytic function of phagocytic erythrocytic. The mouse is injected with epimedium polysaccharide or epimedium flavone subcutaneously to improve the phagocytosis rate of macrophages. In mice with low immune function caused by lymphocyte serum, epimedium can restore the carbon granule phagocytosis ability of macrophages to a normal level. Stimulation of IL-2 production: the herba Epimedii polysaccharide can enhance IL-2 activity produced by human lymphocyte at low concentration (100 μ g/mland1000 μ g/ml). In contrast, at higher concentrations (2500. mu.g/mland 5000. mu.g/ml), Epimedium polysaccharide has an inhibitory effect on IL-2 production. DNA generation and cell propagation of bone marrow cells: the epimedium decoction can obviously improve the propagation (72%) of bone marrow cells and DNA generation (68%) of mice with yang deficiency. Reduction of leukocytopenia symptoms: 22 patients with leukopenia with qi deficiency symptoms take herba Epimedii granule (15 g/bag), 3 bags per day at 1 week and 2 bags per day at 2 weeks for 30-45 days, and 14 patients in 22 patients with stable administration have 3 cases cured, 5 cases effective and 2 cases ineffective. Improving the quality of life of a patient undergoing maintenance hemodialysis: when the epimedium is used for treating dialysis patients for 4 months, the infection incidence rate of the patients tends to be reduced, the activity of peripheral blood lymphocyte hormone 2 is obviously enhanced, and the physical strength, sleep, appetite and the sexual function of male patients are improved. Anti-asthmatic action: an experiment using cat as a model found that epimedium extract completely inhibited cough caused by current stimulation of recurrent laryngeal nerve. In another study conducted in guinea pigs, epimedium was shown to relieve histamine-induced asthma. For treating chronic tracheitis, 1066 cases are treated by single-ingredient epimedium pill, and the total effective rate is 74.6 percent. The antiviral effect is as follows: herba Epimedii has antibacterial and antitoxic effects, and is effective in inhibiting Staphylococcus albus, Staphylococcus aureus, Neisseria catarrhalis, Diplococcus pneumoniae, Haemophilus influenzae, poliomyelitis virus, ECHO virus types 6 and 9, Coxsackie virus types A9, B4, and B5. Is more effective in treating viral myocarditis than vitamin C: 36 cases of treatment are treated by using epimedium extract tablets 7-10 tablets each time and 3 times a day, and simultaneously vitamin C3g is added into 500ml of 10 percent glucose solution for intravenous drip, compared with 25 cases of single treatment of vitamin C, the former has the significant efficiency of 69.44 percent and the latter is 40 percent. Antioxidant effect in animals: the epimedium water extract has obvious inhibiting effect on the generation of the homogenized lipid peroxide of the heart, the liver, the brain and the kidney of a rat. The epimedium flavone for the acute aging mouse model can obviously recover the mouse T and B lymphocyte proliferation reaction, obviously improve the activity of (SOD) under the mouse liver total superoxide dismutase, reduce the formation of liver tissue lipid peroxide, and reduce the formation of lipofuscin in tissues such as heart, liver and the like. Other studies have shown that icariine has catecholamine antagonistic action. Hypoglycemic effects in animals: the epimedium extract for gavage of normal rats or experimental hyperglycemia rats has the function of reducing blood sugar.

In addition, epimedium has wide clinical application. Herba Epimedii can be used for treating sexual impotence, spermatorrhea, tendons and bones flaccidity, rheumatism, numbness, spasm, climacteric hypertension, and osteoporosis. The stem and leaf of herba Epimedii contain icariine and volatile oil. It is proved that epimedium has the action of male hormone, and its effect is stronger than that of gecko and hippocampus. Clinically, it has been shown that it stimulates libido and penile erection by promoting the secretion of seminal fluid, which in turn stimulates sensory nerves after the seminal vesicles are filled with seminal fluid. Meanwhile, the epimedium can also inhibit the vasomotor center, expand peripheral blood vessels, lower blood pressure, relieve cough, eliminate phlegm and relieve asthma, and has obvious inhibiting effect on poliovirus, white staphylococcus, staphylococcus aureus and the like. Herba Epimedii is mainly used for treating diseases of reproductive system, bone joint and respiratory system in clinic. Herba Epimedii can be combined with radix rehmanniae Preparata, radix Angelicae sinensis, Atractylodis rhizoma, fructus Lycii, Eucommiae cortex, rhizoma Curculiginis, radix Morindae officinalis, Corni fructus, fructus Cnidii, semen Allii Tuberosi, Cistanchis herba, radix Aconiti lateralis Preparata, and cortex Cinnamomi to obtain Zanyu pill for treating sexual impotence and premature ejaculation. Herba Epimedii can be combined with radix Clematidis, fructus Xanthii, and rhizoma Ligustici Chuanxiong to treat arthralgia. Herba Epimedii is not limited in size, and it can be decocted for treating toothache. The decoction prepared from herba epimedii and Japanese ardisia herb can be used for treating chronic bronchitis, and has obvious effects of eliminating phlegm and relieving cough. Herba Epimedii is combined with radix astragali, radix Codonopsis, radix Aconiti lateralis Preparata, herba asari, and herba Ephedrae by decocting to obtain decoction for treating sick sinus syndrome and atrioventricular block. Herba Epimedii is used for treating poliomyelitis, and is prepared into injection containing crude drugs 1g each per 2 ml, with the same amount. In the acute stage, intramuscular injection is mainly used, and acupoint injection is matched. Intramuscular injection of 2 ml each time, 2 times daily for 20 consecutive days. In the convalescent period and the sequela period, acupoint injection is mainly used in combination with intramuscular injection. The acupoint injection is carried out according to the conventional method, 1-2 ml of the injection is injected into each acupoint, every other day is carried out for 1 time, the treatment is continuously carried out for 20 days, and the treatment is continued after half a month of rest. 246 cases of poliomyelitis in each stage are treated, wherein 34 cases, 8 cases, 16 cases, 7 cases, 2 cases and1 case are basically cured in the acute stage; 43 patients in convalescent period, 21 patients are cured, 15 patients are effective, 5 patients are advanced, and 2 patients are ineffective; in 169 patients in the sequela stage, 9 patients are cured and basically cured, 129 patients are obviously effective and effective, and 31 patients are not effective. According to observation, the product has remarkable curative effect on acute stage and recovery stage of patients, and can recover quickly. The traditional Chinese medicine composition also has a certain effect on the sequela stage, the affected limb generally has the feeling of fever, strength and the like after the medicine is taken, and the muscle is gradually recovered and the affected limb is thickened in the effective case; among them, the curative effect is better for the patients with paralysis of lower limbs, and the curative effect is worse for the patients with paralysis of upper limbs and older age. After the medicine is taken, no other side effects except the phenomenon of dry mouth are seen. Herba Epimedii is used for treating neurasthenia by 3% herba Epimedii bud decoction for penetration, 1 time daily, and 10-20 times as a treatment course. A small number of patients receive additional sedatives. 104 cases were observed, 22 cases were recovered, 21 cases were significantly improved, 46 cases were improved, and 15 cases were not effective. The curative effect is better for patients with long treatment course; the symptoms begin to improve earlier after treatment, the curative effect is better, and the disease condition still does not improve after 15 times of treatment, and even if the treatment is continued, the curative effect is not obvious and is not consolidated. In a few cases, mild reactions or temporary exacerbations of symptoms may occur initially, but they disappear rapidly upon continued treatment. The 20% epimedium tincture is taken 3 times a day before meals for 2-3 months continuously, and 5ml of the tincture is taken every time. In 50 cases, about 80% of patients have eliminated or reduced symptoms after treatment. Adverse reactions are not seen during the administration, but the medicine is not suitable for patients with dominant excitation process or hypersexuality and hypertension. Herba Epimedii can be used for treating chronic tracheitis, and has effective rate of 74.6% and recent control and significant efficiency of 22.1% in over 1000 cases. If the composition is combined with Japanese ardisia herb to form compound treatment, the curative effect is improved. Practice proves that the simple form has better curative effect than the asthma form, and the old has poorer curative effect, but the disease course length has no obvious relation with the curative effect; good expectorant, antitussive and antiasthmatic effects; the recent control and the obvious efficiency of the patient treated by two courses of treatment are obviously improved compared with those treated by one course of treatment. In observation, 110 cases treated in one treatment course are followed up, the effective rate after half a year is 59.1 percent, and the curative effect is reduced by 26.1 percent compared with the original curative effect. The preparation and the use method are as follows: decocting stem and leaf of herba Epimedii (dried product) 80% of the total amount to obtain concentrated juice, grinding 20% of the concentrated juice into powder, and mixing the two powders to obtain pill. The daily dose is equivalent to 1 two of crude drugs, and the crude drugs are taken twice. In the treatment, the treatment was carried out in a dose (daily dose) equivalent to 5X and 1.5H of the crude drug, and the treatment effect was substantially similar to that of the daily dose 1. After the medicine is taken, some cases have slight reaction, dry mouth and nausea are common, and abdominal distension and dizziness are secondary symptoms, and the symptoms generally disappear by self.

Cornu Cervi Pantotrichum, Cornu Cervi Pantotrichum, Cornu Cervi Pantotrichum, east Cornu Cervi Pantotrichum, and stigma croci Sativi, Cervidae, and Cervidae elaphus androgynous young Cervidae of male deer of animal Cervus Elaphus, are mainly inhabited in mountain forest grassland. According to the shape of antler, hairy antler can be divided into lotus antler, three-twig antler and four-twig antler. The cervus elaphus linnaeus comprises the following chemical components: contains acidic mucopolysaccharide, polyamine, and various amino acids. Property, flavor and channel tropism of cervus elaphus linnaeus: warm in nature, sweet and salty in taste. The cervus elaphus linnaeus has the main functions: strengthen kidney yang, replenish essence and blood, strengthen tendons and bones, regulate thoroughfare and conception vessels, and relieve sore toxicity. Can be used for treating sexual impotence, spermatorrhea, infertility due to cold womb, emaciation, listlessness, aversion to cold, vertigo, tinnitus, deafness, lumbago, muscle and bone atrophy, metrorrhagia, leukorrhagia, and dorsal furuncle. The pharmacological action of cervus elaphus linnaeus is as follows: the flow of coronary artery of isolated heart of rat is increased, the heart contraction amplitude is increased, the heart rate is slowed down, and the heart strengthening effect is achieved; can accelerate the recovery of acute hemorrhagic hypotension. In addition, it also has effects of resisting anoxia, improving intelligence, strengthening body, promoting glycolysis, and promoting nucleic acid and protein synthesis.

The cervus elaphus linnaeus has very strong health-care effect and is a good body-building and strengthening medicine. The cervus elaphus linnaeus contains more abundant amino acids, lecithin, vitamins, trace elements and the like than the ginseng; the cervus elaphus linnaeus is warm in nature but not dry, has the functions of revivifying and improving the organism, and has better health-care effect on patients with general weakness and after long-term illness. Cornu Cervi Pantotrichum has effects of improving cellular immunity and humoral immunity, promoting lymphocyte transformation, and promoting immunity. It can enhance the body's defense ability against the external world, regulate the immune balance in the body, thus avoid the occurrence of diseases, promote the healing of wounds and the recovery of sick bodies, thus playing the role of strengthening the body and resisting aging. Action of cervus elaphus linnaeus on nervous system: cervus elaphus linnaeus can enhance the tension of parasympathetic nerve endings, promote recovery of the nervous system and improve the functions of the nervous system and the muscular system, and has an excitation effect on sympathetic nerves. Action of cornu Cervi Pantotrichum on cardiovascular system: the cornu Cervi Pantotrichum with large dosage can reduce blood pressure, reduce amplitude of heart contraction, slow heart rate, and dilate peripheral blood vessel. The moderate dose can cause the heart to shrink obviously and increase the contraction amplitude and the heart rate, thereby increasing the cardiac output; the cervus elaphus linnaeus has particularly remarkable effect on tired heart. The function of cervus elaphus linnaeus on sexual function: the cornu Cervi Pantotrichum extract can increase testosterone concentration in blood plasma and Luteinizing Hormone (LH) concentration. Therefore, the cervus elaphus linnaeus is effective in treating sexual dysfunction in adolescence and prostatic atrophy in the old and strong period; has good effect on treating female climacteric disorder. Strengthening action of cervus elaphus linnaeus: cervus elaphus linnaeus essence has strong anti-fatigue effect, and can enhance cold resistance, accelerate wound healing and stimulate adrenal cortex function. Therefore, cervus elaphus linnaeus is a traditional tonifying medicine and is used for strengthening, tonifying kidney and tonifying yang. Cervus elaphus linnaeus can increase hemoglobin in blood, so that the cervus elaphus linnaeus is extremely effective for treating patients with massive bleeding and patients at the terminal stage of infection, particularly for old patients. Action of cervus elaphus linnaeus on sex hormones: animal experiments prove that the cervus elaphus Linnaeus tincture is used for subcutaneous injection, and the weight of prostate and seminal vesicle is increased after several days. Cervus elaphus linnaeus is a nourishing product, but not all people are suitable for being nourished, and the cervus elaphus linnaeus cannot be eaten by healthy people, particularly children. When a healthy child eats the food, internal heat, nosebleed and abnormal hyperactivity of the body even mania occur easily. The function of cervus elaphus linnaeus on intestinal canal and uterus: enhancement of diuretic function of kidney: the cervus elaphus linnaeus is beneficial to women and children, and inorganic elements such as calcium, phosphorus, magnesium and the like contained in the cervus elaphus linnaeus respectively participate in the metabolism of calcium and phosphorus and various enzymes of the body, and have positive effects on promoting the absorption of calcium, the growth of bones and enhancing the functions of heart and muscle. Especially, magnesium ions have complex functions and are indispensable substances for prolonging life. It has good therapeutic effect on female infertility due to cold womb, menoxenia, infantile dysplasia, and delayed tooth growth, fontanel incompatibility, and bone softness due to kidney qi deficiency. It is noted that patients with hypertension, nephritis, hepatitis, and hyperactivity of liver-yang due to yin deficiency and hyperactivity of fire in traditional Chinese medicine should not take the medicine. Action of cervus elaphus linnaeus on treating wounds: cervus elaphus linnaeus extract has the function of strengthening tendons and bones, and is used for treating 'wound, carbuncle and swelling'. Tests prove that the cervus elaphus linnaeus has the capability of enhancing tissue regeneration on ulcer and wound which are not healed for a long time and have poor regeneration, and the healing of fracture is promoted. The oral or topical application of cornu Cervi Pantotrichum can promote wound healing, especially suppurative wound. Other functions of cervus elaphus linnaeus are as follows: some effective components in cornu Cervi Pantotrichum can inhibit MAO-B activity, so it has antiaging effect; cornu Cervi Pantotrichum has antioxidant effect, and can enhance gastrointestinal motility and promote secretion; in addition, cervus elaphus linnaeus essence can also enhance the immune function of the body and the like.

In the invention, the cordyceps militaris is a model species of ascomycota, hypocotyledoneae, ergomycetaceae and cordyceps. The scientific name is Cordyceps militaris (L.ex Fr.) Link, called Cordyceps militaris, or Cordyceps militaris for short, generally the Cordyceps militaris cultured by living pupa is called Cordyceps militaris, the two are the same kind of fungus, but the content difference of the two is large. Cordyceps militaris is distributed worldwide, and the quantity of natural resources is small. In 1950, German scientist Cunningham observed that insect tissue parasitized by Cordyceps militaris is not easy to rot, and further separated an antibacterial substance, 3' -deoxyadenosine, named as cordycepin. Cordyceps militaris, pupa of a multiply-infected lepidopteran insect, is a complex composed of a stroma (i.e., a grass part, also called a fruiting body) and a sclerotium (i.e., a corpse part of the insect), and simply speaking, is the combination of the body and the grass. The traditional Chinese medicine considers that the traditional Chinese medicine can tonify lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, soreness and pain of waist and knees, weakness after illness, chronic cough and weakness, cough with phlegm and blood caused by overexertion, spontaneous perspiration and night sweat and the like, and is a traditional Chinese medicine capable of balancing and regulating yin and yang simultaneously. Morphological characteristics of cordyceps militaris: the stroma grows out singly or together from the head or the node of the parasitic pupa body, the color is orange or orange, the total length is 2-8 cm, and the color of the pupa body is purple, and the length is about 1.5-2 cm. Mycelium: cordyceps militaris is a kind of ascomycete, and sexual reproduction is carried out by using the heterothallic cooperation. The non-sexual type is paecilomyces chrysalis. The mature sporocarp can form ascospore (propagation unit), the spore spreads with wind, the spore falls on proper insect body, and starts to germinate to form mycelium. The mycelium continuously develops and begins to spread into the pupa body, so that the pupa body can be infected by fungi, the tissue in the pupa body is decomposed, the nutrition in the pupa body is used as a material and an energy source for the growth and development of the pupa body, and finally, the interior of the pupa body is completely decomposed. Fruiting body: generally, after the mycelium of Cordyceps militaris decomposes various tissues and organs in pupa, the development of mycelium also enters a new stage to form a rod-shaped stroma (fruiting body) with an orange or red-orange top and a slightly enlarged top.

Cordyceps militaris is found in China in Jilin province for the first time in 1958, and through identification, the cordyceps militaris belongs to the same genus as cordyceps militaris and is named as cordyceps militaris. Mainly produced in Yunnan (Kunming, Anning, Jiangchuan), Jilin (AnTu, Yongji), Liaoning (Shenyang), inner Mongolia (Taimen union), and on pupa bodies of lepidopteran insects in the surface soil layer of acupuncture, broadleaf forest or mixed forest. Compared with cordyceps sinensis, cordyceps militaris has several incomparable advantages: firstly, the cordyceps militaris is a model species of cordyceps, is widely distributed and is recognized and accepted by various national scholars in the world; secondly, the cordyceps militaris has been bred into a complete stroma under artificial conditions; thirdly, the cordyceps militaris contains cordycepin and cordyceps polysaccharide, and the unique pharmacological action of the cordyceps militaris draws high attention from the pharmaceutical industry increasingly. As the cordyceps militaris has the advantages, the cordyceps militaris is superior to cordyceps militaris which is a traditional Chinese medicine cordyceps fungus. In fact, in general, a particular organism has a particular biochemical reaction that produces a particular biological substance. Regarding cordyceps sinensis, various nutrient components can form various bioactive substances such as cordycepin, cordycepic acid, cordyceps polysaccharide and the like only through biochemical actions of organism absorption and synthesis of cordyceps sinensis, and other biological methods or artificial chemical methods cannot be achieved simultaneously. The artificial chemical method enables synthesis of one of them but is difficult to synthesize a plurality of them simultaneously. Cordyceps sinensis is a bioreactor, and the synthesized bioactive substances are the same regardless of the production method. The bioactive substances have the same health and medicinal value. Cordycepin, cordycepic acid and cordyceps polysaccharide are special substances of cordyceps sinensis and are main indexes of special efficacy of cordyceps sinensis. Just these indexes are that the content of Cordyceps militaris grown under the condition of artificial environment is superior to that grown under the natural condition. The contents of protein, amino acid, vitamin and the like in the artificially cultured cordyceps militaris all reach or exceed the level of natural cordyceps militaris. Although the individual nutrients in artificially cultured Cordyceps militaris are slightly lower than the levels of natural Cordyceps sinensis, these nutrients can be improved by research. The cordyceps sinensis, the ginseng and the cervus elaphus linnaeus are all famous and abroad as traditional three-major tonic products in China, and research on nutritional chemical components, pharmacological and clinical effects of the cordyceps militaris by a plurality of experts at home and abroad is basically the same as that of the cordyceps sinensis, and the cordyceps sinensis mainly has the effects of protecting lung and tonifying kidney, stopping bleeding and reducing phlegm, regulating body functions, mainly treating phthisis, hemoptysis, impotence and spermatorrhea, soreness and pain of waist and knees, beautifying and protecting skin, neurasthenia, diabetes, leucocyte increasing, cancer prevention and cancer resistance and also has great effect on chemotherapy. The medicinal efficacy of the artificially cultivated cordyceps militaris is almost the same as that of wild cordyceps militaris, and the medical performance of some aspects is even better than that of the wild cordyceps militaris. The pharmaceutiques, pharmacology and clinical experiment prove that the cordyceps militaris can be completely used as a substitute of cordyceps sinensis. The research proves that the artificially cultured cordyceps militaris has the same clinical effects as natural cordyceps militaris, can also regulate the functions of the whole body, improve the immunity, enhance the phagocytic function of macrophages and promote the formation of antibodies, and mainly has the effects of protecting the lung, tonifying the kidney, nourishing the liver, relieving cough, relieving asthma, eliminating phlegm, moistening the skin, preventing wrinkles and aging, resisting bacteria, resisting inflammation, calming, expanding blood vessels, reducing blood pressure, reducing blood sugar, resisting fatigue, resisting oxygen deficiency and the like. The artificial cordyceps militaris is mainly used: deficiency tuberculosis hemoptysis, soreness of waist and knees, sexual impotence, spermatorrhea, neurasthenia, palpitation, insomnia, night sweat, arrhythmia, anorexia, nasopharyngeal carcinoma, emphysema, pulmonary tuberculosis, tracheitis, asthma, hepatitis, nephritis, kidney essence deficiency, diabetes, frequent micturition, and leukopenia after radiotherapy and chemotherapy.

Cordyceps militaris contains a large amount of cordycepin, the special medical health-care function of cordycepin draws high attention of experts at home and abroad, and a plurality of health-care products, health-care food, cosmetics, medicines and the like mainly containing cordycepin are put on the market. New drugs synthesized from cordycepin for treating leukemia have also been tried in clinical trials at the entry stage in China. The health-care functional components of the cordyceps militaris not only contain cordycepin, but also contain cordyceps polysaccharide, which is a human body immunopotentiator recognized by international medicine. The traditional Chinese medicine considers that the traditional Chinese medicine has the effects of strengthening body resistance and consolidating constitution, has obvious curative effects on senile chronic bronchitis and pulmonary heart disease, can improve the detoxifying capability of the liver, has the effect of protecting the liver, and improves the antiviral and anti-radiation capability of the body. Cordycepic acid, D-mannitol, is a basic medicine for treating cardiovascular and cerebrovascular diseases, and has effects of scavenging free radicals, dilating blood vessel, and lowering blood pressure. The nucleotide has effects of inhibiting blood platelet aggregation, preventing heart and brain thrombosis, eliminating chloasma, senile plaque and acne, resisting aging and wrinkle, caring skin, etc. The artificially cultured pure fruit body of cordyceps militaris is used as food for human bodies, is completely safe through research, and can be widely used for health-care food, health-care diet and other nourishing foods. Meanwhile, the artificially cultured cordyceps militaris sporocarp is bright in color, pure in smell, high in practicability and high in commodity value, and is suitable for various different people. Cordyceps militaris can also be combined with some traditional Chinese medicines, and has therapeutic effect on some diseases. The culture medium residue generated in the process of artificially cultivating the cordyceps militaris can also be added with 5 to 10 percent of culture medium residue in the raw materials for brewing the soy sauce, and the brewed soy sauce has pure taste, rich nutrition and rich cordyceps sinensis fragrance.

The biological effects of cordyceps militaris mainly comprise: 1. strengthen body resistance, replenish qi, improve immunity and hematopoiesis. 2. Tonify lung, relieve asthma, and improve respiratory system. ⒊ can improve and regulate cardiac and cerebral systems. 4. Regulating blood lipid in vivo. 5. Has the effects of tonifying lung and kidney, and improving immunity and brain memory. 6. Has effects of invigorating spleen, tranquilizing mind, and treating insomnia, dreaminess, and neurasthenia. The chemical components of the cordyceps militaris mainly comprise: n6- [ β - (acetamidoformyl) oxyethyl ] adenosine; cordyceps sinensis cyclopeptide A; a nucleoside; ergosterol peroxide; cordycepin, cordycepic acid, cordyceps polysaccharide and cordyceps SOD. The medicinal value of various bioactive substances such as cordyceps polysaccharide, SOD and the like is most obvious. Cordycepic acid (mannitol) can remarkably reduce intracranial pressure and promote metabolism, thereby relieving cerebral hemorrhage and cerebral thrombosis. Cordycepin is a nucleoside substance with antibacterial activity, and has strong inhibitory effect on nuclear polyadenylic acid polymerase. Make mRNA mature in the process of DNA transcription mRNA and inhibit the growth of cancer cells. And has blood sugar lowering effect. Cordyceps polysaccharide is a highly branched galactomannan, and can promote lymphocyte transformation, improve antibody content of serum IgG and immunity of organism, and enhance anticancer ability of organism. SOD (superoxide dismutase) can eliminate superoxide radical in organism, and has antiaging, anticancer and anticancer effects. In addition, the cordyceps militaris also contains rich selenium (Se), which is well known to be a trace element necessary for human bodies, is an active center of glutathione peroxidase, is connected to a peptide chain of enzyme protein in the form of selenocysteine, protects the normal permeability of the stability of cell membranes, stimulates the production of immunoglobulin and antibody, and enhances the immunity and the antioxidant capacity of organisms. Meanwhile, a great deal of scientific practice proves that selenium can obviously inhibit the growth of cancer cells. Cordycepin (3' -deoxyadenosine) has the following functions: antivirus, antibacterial, inhibiting tumor growth, interfering RNA and DNA synthesis of human body. Cordycepic acid (D-mannitol) has the following functions: preventing and treating cerebral thrombosis, cerebral hemorrhage, renal failure, and promoting urination. Adenosine has the following effects: has antiviral and antibacterial effects, and can be used for preventing and treating cerebral thrombosis and cerebral hemorrhage, inhibiting platelet accumulation, preventing thrombosis, eliminating facial speckle, resisting aging, and preventing wrinkle. The cordyceps polysaccharide has the following functions: : improving immunity, delaying aging, strengthening body resistance, consolidating constitution, protecting heart and liver, and relieving spasm. Ergosterol has the following effects: anticancer, antiaging, and toxic materials clearing away. Superoxide dismutase (SOD) has the following effects: inhibiting or eliminating formation of superoxide radical which promotes aging, resisting cancer, delaying aging, and reducing toxicity. Selenium (Se) has the following effects: is an anticancer element recognized by the international medical community, is also an important antioxidant and can enhance the immunity of human bodies.

In the invention, Cistanche salsa (the scientific name: Cistanches deservicola Ma), namely Jiangxu, Cistanchis herba and Charles abraham (Mongolian), belong to endangered species. Big and tall herbs, 40-160 cm tall, mostly grown underground. The flowering period is 5-6 months, and the fruit period is 6-8 months. Mainly produced in Sinkiang and the inner Mongolia AlaLau, and also distributed in Gansu and Ningxia. Cistanche salsa is a parasitic plant parasitic on the root of haloxylon ammodendron in desert trees, and absorbs nutrients and water from haloxylon ammodendron hosts. The ginseng has the reputation of desert ginseng, has extremely high medicinal value and is a traditional famous and precious Chinese medicinal material. Cistanche deserticola has historically been used as a treasure in Shang Gong court in countries in Western countries and is also one of the most frequently used tonifying medicines in the formulas for tonifying kidney and strengthening yang in all ages. The succulent stem contains cistanoside A, B, C, H, eueugenol glycoside (acteoside), 2-acetyleueugenol glycoside (2-acteoside), echinacoside (echinacoside) seven kinds of phenylethanol glycoside components, lirio-dendrin, 8-epinuaridic acid (8-epiloganic acid), daucosterol (daucosterol-terol), betaine (betaine), beta-sitosterol (beta-sitosterol), mannitol (mannitol), N, N-dimethylglyceric acid methyl ester (N, N-dimethylglycero ester) and phenylalanine (phenylalkane), valine (valine), leucine (leucine), isoleucine (isoluteine), lysine (lysine), threonine (serine) and other fifteen kinds of amino acids, succinic acid (succinic acid), triacontanol (TCtriacontanol), and polysaccharides. Cistanche deserticola is warm in nature, sweet and salty in taste; it enters kidney and large intestine meridians. The cistanche deserticola has the main functional indications that: tonifying kidney yang; replenishing essence and blood; moistening intestinal tract. Deficiency of both the major and kidney yang; impotence due to deficiency of essence and blood; spermatorrhea; white turbidity; frequent micturition and dribbling; lumbago and weak feet; tinnitus and blurred vision; menstrual period of menstruation; infertility due to cold uterus; constipation due to intestinal dryness. Since the 20 th century and the 80 th century, a great deal of research on the components of cistanche has been carried out at home and abroad, wherein various types of substances are separated from the beginning of Japan along with the rapid development of separation, extraction and detection technologies, the substances mainly comprise phenylethanoid glycosides, iridoids, lignans, polysaccharides, dozens of amino acids, various alkaloids and the like, are rich in trace elements required by a human body, and the phenylethanoid glycosides are main active ingredients in the cistanche and have the functions of improving male sexual functions, treating female infertility caused by cold uterus, resisting oxidation, resisting aging, improving immunity, enhancing memory and the like.

The pharmacological action of cistanche deserticola is mainly reflected in the above aspects. Improving immunity: cistanche deserticola belongs to a rare medicinal material which can excite pituitary and adrenal cortex or has the similar effect of adrenal cortex hormone and can regulate the immunologic function of organisms, the polysaccharide has higher content in extracted species, and the immunologic enhancement effect of the polysaccharide component can play a main role in strengthening and tonifying the cistanche deserticola. Effects on the immune system: the cistanche aqueous extract 50mg/kg and100 mg/kg is used for gastric perfusion of mice, so that the weight of spleen and thymus can be obviously increased, the phagocytic capacity of abdominal cavity macrophages can be obviously enhanced, hemolysin and PFC (Power factor correction) values can be increased, the lymphocyte transformation rate and delayed hypersensitivity index can be improved, the cAMP level can be increased, the cGMP level can be reduced, the cAMP/cGMP ratio can be increased, and the method is probably one of reasons for enhancing the phagocytic function of the abdominal cavity macrophages. The ethanol extract of cistanche deserticola 1.25g/kg and 2.5g/kg (equivalent to crude drug 5.61g/kg and 11.21g/kg) are administered to mice by intragastric administration, and are sensitized with sheep red blood cells for 8 days continuously and5 days, so that the number of plaques in splenocytes can be obviously inhibited. Herba cistanches extract and herba Epimedii total flavone have additive effect in promoting stranguria of mouse with immune depression, and can enhance immunity of model mouse. Anti-aging effect: cistanche salsa has a certain anti-aging effect. Cistanchis herba can obviously enhance the activity of mouse erythrocyte superoxide dismutase (SOD) and obviously reduce the content of myocardial lipofuscin in mouse. Can also prolong the average life span, half of death days and the maximum life span of the fruit flies. The cistanche decoction 8g/kg is used for gastric perfusion of mice, and can obviously increase the activity of Na +, K + -ATP enzyme of erythrocyte membranes, which is probably one of the mechanisms of the tonifying effect. In 1993, a news story was released at the international medical society, geneva: the AlaLain union of China examines the township of the dry Hi-Hot ascending world. During rush, checking the heat of dryness becomes a focus of attention. The panel of Chinese and foreign experts took 4 months to investigate the heat of dry eyes. They found that only 139 people in the manshu hui-hui (gacha) village had 4 people in centenarian, and the average life span was 87.5 years old; the incidence of hypertension, gastropathy, nephropathy, prostatic hyperplasia and other common diseases is very low. The expert group finds that the dietary structure of local residents is related, wherein two points are that cistanche salsa is put into the mutton stewing habit of people and cistanche salsa wine brewed from home is also taken. Improving impotence and premature ejaculation: tang Dynasty "materia Medica Shi" recorded: three-money cistanche, three-decoction one-preparation, hot drinking and no decline of yang life. Modern medicine also proves that cistanche deserticola contains a large amount of rare nutrient and nourishing components of amino acid, cystine, vitamins and mineral substances, has great tonifying effect on male sexual organs such as kidney, testis, penis, cavernous body and the like, and can also effectively improve the vitality and quality of sperms. And (3) fatigue resistance: kidneys store essence, dominate bones and produce marrow, which is the innate root and the prime and source of physical strength. Prolonged exercise, excessive exertion, can cause a major factor in the decline of blood testosterone in the physical exercise capacity and the prolongation of the recovery process. Herba cistanches can prevent and treat blood testosterone reduction caused by exercise, promote secretion of pituitary gonadal hormone, accelerate fatigue recovery, and improve exercise ability. Also can reduce the accumulation of blood lactic acid, accelerate the removal and improve the acid resistance of the organism after quantitative loading exercise. Protecting the liver: cistanchis herba has various pharmacological activities of protecting ischemic myocardium, reducing blood lipid, resisting atherosclerosis and thrombosis, reducing peripheral vascular resistance, lowering blood pressure, resisting fatty liver, resisting tumor, etc. Cistanchis herba can promote deoxyribonucleic acid synthesis, and Cistanchis herba decoction (0.5g/ml) can be administered by intragastric administration for treating mice with yang deficiency caused by hydrocortisone acetate, and can increase synthesis of deoxyribonucleic acid in liver and spleen. Regulating endocrine, promoting metabolism and strengthening effects: herba cistanches has effects of regulating decrease and increase of nucleic acid content in liver and spleen of animals with yang deficiency and yin deficiency; has the effects of activating adrenal gland and releasing corticoids. The mechanism of action may be to enhance the hypothalamic-pituitary-ovarian luteinizing function not because they directly stimulate the secretion of pituitary luteinizing hormone, but rather to increase the reactivity of the pituitary to LRH and the reactivity of the ovary to LH. The main significance of the traditional Chinese medicine for tonifying the kidney is to improve the regulation mechanism in the organism, play a positive role in treatment by the restoration of the intrinsic function of the organism, and discover through the research on the effect of the cistanche on the DNA synthesis rate of the animal with the syndrome of yang deficiency, that the DNA synthesis rate is reduced due to the reduction reaction of hydroxyurea which inhibits nucleotide at the diphosphorylation level, and that the disorder of protein and energy metabolism causes the syndrome of yang deficiency; the cistanche deserticola probably contains a bioactive factor for activating nucleotide reductase, so that the DNA synthesis rate of the animal with yang deficiency can be obviously improved, and the RNA synthesis is promoted; increasing the nucleic acid metabolism of the protein; the diluted ethanol extract is added into drinking water to breed rat, and has growth promoting effect. Protecting the cardiovascular and cerebrovascular systems: it is especially obvious in late autumn that many people easily get cold in hands and feet and the limbs are intolerant of cold. The active ingredient of the phenethyl alcohol total glycoside in the cistanche deserticola improves microcirculation, softens blood vessels, increases the circulation of peripheral blood vessels of heart, brain and outer limbs, and achieves the effect of protecting blood vessels, blood vessels of brain and nervous system. Moistening intestines and expelling toxin: the traditional Chinese medicine considers that the cistanche deserticola enters kidney and large intestine channels, and tonifies the kidney and strengthens yang to moisten dryness and relax bowels. The decoction of cistanche drugs has obvious function of relaxing bowels, can improve intestinal peristalsis, inhibit water absorption of large intestine and shorten defecation time. Has obvious curative effect on habitual constipation, body weakness constipation and puerperal constipation of the old. Other functions are as follows: the water extract, ethanol-water extract and ethanol extract of Cistanchis herba have blood pressure lowering effect on anesthetized dog, cat, rabbit, etc., and Cistanchis herba has effects of promoting salivary secretion and respiratory paralysis for mouse, wherein the component for promoting salivary secretion is certain organic acid-like substance, and the component for respiratory paralysis may be glycosides.

In the preparation of the composition of the present invention, it has been unexpectedly found that after the cordyceps militaris has been homogenized with a small amount of xylitol and water in step (3) and then treated at 50 ℃, the adenosine content in the resulting oral liquid is higher than that without this treatment, and that the adenosine content in the filter cake obtained by filtering in step (4) after the treatment with the method of step (3) above (although the amount of filter cake is very small, its weight is about 2.3-3.1% of the input amount of cordyceps militaris, and this filter cake should not be entirely attributable to the production of cordyceps militaris) is determined to be lower, indicating that the residual amount of adenosine in the filter cake after the treatment with the method of step (3) above is significantly lower. The method for measuring adenosine content in various materials is carried out according to the method of [ content measurement ] of Cordyceps sinensis carried on page 115 of the first part of the Chinese pharmacopoeia 2015 edition. The specific test is as follows: referring to examples 1 to 5, respectively, except that xylitol was not added in the step (3), 5 batches of oral liquids, which may be referred to as supplement examples 1 to 5, respectively, were obtained; for each sample, determining the percentage of adenosine content in the sample to the theoretical adenosine content in the cordyceps militaris feeding amount, and taking the percentage as the adenosine recovery rate, the adenosine recovery rate of the examples 1-5 is 96.7-99.3%, and the adenosine recovery rate of the supplement examples 1-5 is 86.2-89.5%; further, the adenosine loss rate due to the interception of the filter cake was calculated by measuring the amount of adenosine in the filter cake obtained after the filtration in step (4) in examples 1 to 5 and supplementation examples 1 to 5, and as a result, the adenosine loss rate in examples 1 to 5 was in the range of 0.6 to 3.5%, and the adenosine loss rate in supplementation examples 1 to 5 was in the range of 10.1 to 13.3%. These results show that adenosine recovery is high after treatment by step (3) of the present invention.

In the composition of the present invention, it has been unexpectedly found that the stability of three ginsenosides, which are important active ingredients in the oral liquid, can be significantly improved by adding a small amount of sodium tartrate, and it has been found through experiments that the stability of the three ginsenosides may be affected by the added sugar alcohol. The specific method and results for improving the stability of the three ginsenosides are as follows: referring to examples 1-5, respectively, except that in step (1), sodium tartrate (the addition amount of each example is 10%, 15%, 5%, 12%, 8% of the weight of the ginseng extract) is further added into the ginseng extract paste prepared in step (1) to obtain 5 batches of oral liquid which can be called supplement examples a 1-a 5; the oral liquids of example 1 to example 5 and the oral liquids of supplement a1 to supplement a5 were allowed to stand at 40 ℃ for 6 months, and the total content of three ginsenosides in each oral liquid at 0 month and 6 months was determined by the [ content determination ] method for ginseng carried on page 8 of the first part of the "chinese pharmacopoeia" of the 2015 edition; the percent residual total saponin content was calculated for each sample according to the following formula: the total content of saponin residue percentage is 6 month saponin total content ÷ 0 month saponin total content x 100%; as a result, the total content percentage of the saponins in the oral liquid of the embodiments 1 to 5 is 88.7 to 90.3 percent, the residual percentage of the total content of the saponins in the oral liquid of the supplementation examples a1 to a5 is 97.4 to 99.0 percent, and the later is higher than the former, which shows that the ginsenoside which is an important physiologically active substance in the oral liquid of the later is obviously more stable; in addition, referring to examples 1 to 5, respectively, except that xylitol is not added in the formula, 5 batches of oral liquid which can be called supplement examples b1 to supplement examples b5 are obtained, the five batches of oral liquid are treated for 40-6 months by the above method, the residual percentage of the total content of the saponin at 6 months is measured and calculated, and the residual percentage of the total content of the saponin is 96.8-98.3%. This indicates that the significant decrease in total saponin content in examples 1-5 may be adversely affected by xylitol, and this adverse effect can be overcome by the addition of the pharmaceutical adjuvant sodium tartrate, which is conventional in the art. Thus, according to any aspect of the invention, wherein sodium tartrate is also added. In one embodiment, the amount of sodium tartrate added is 5 to 15% by weight of the ginseng extract. In one embodiment, sodium tartrate is added to the obtained ginseng extract fluid extract.

The composition for delaying senility, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation is prepared from ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche deserticola and cervus elaphus linnaeus. Presents the combined biological efficacy of the medicinal materials, and particularly has the obvious efficacies of delaying senility, inhibiting the growth of tumor cells and improving cardiovascular and cerebrovascular microcirculation. For example, for 18 men aged 45 to 50 and 15 men aged 48 to 53, each person administered 30ml of the composition obtained in example 1 of the present invention every day was taken twice a day, and after taking the composition for one quarter, the spirit was remarkably refreshed and the facial wrinkles were remarkably reduced compared to before taking the composition of the present invention, indicating that the composition of the present invention has an excellent effect of delaying aging. For another example, for 26 benign tumor patients, 30ml of the composition obtained in example 1 of the present invention was administered to each person twice a day, and after half a year of administration, the patients were significantly more energetic and had much improved sleep, which may be associated with the inhibition of tumor cell growth, than before the administration of the composition of the present invention, indicating that the composition of the present invention has an excellent effect of inhibiting tumor cell growth. For another example, for 28 patients suffering from long-term cerebrovascular microcirculation diseases, each person takes 30ml of the composition obtained in example 1 of the invention twice a day in the morning and at night, and after taking the composition for half a year, various troubles caused by cerebrovascular microcirculation are obviously reduced compared with the cases before taking the composition, which shows that the composition has excellent effect of improving cardiovascular and cerebrovascular microcirculation.

In addition, based on the physiological properties of various medicinal materials in the composition for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation, the composition for delaying senescence, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation can be expected to have biological effects generated by the medicinal materials individually or in combination. For example, the composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation according to the present invention can be endowed with respective typical biological effects of ginseng, cordyceps militaris, prepared rehmannia root, epimedium herb, cistanche deserticola and cervus elaphus linnaeus as described above. Thus, the use of the third aspect of the present invention also includes the uses relating to these therapeutic/prophylactic effects described above.

Detailed Description

The present invention will be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be understood by those skilled in the art that various changes and modifications may be made to the invention without departing from the spirit and scope of the invention. The present invention has been described generally and/or specifically with respect to materials used in testing and testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible.

The total charge per batch in the following composition was 10 kg.

Example 1: preparation of oral liquid composition

The formula is as follows: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus, 8 parts of xylitol, water and a proper amount, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 8 g.

The preparation method comprises the following steps: (1) decocting radix Ginseng 6 times of water for 1.25 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 7 times of water for 12 hr, decocting for 1.25 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (3) mixing Cordyceps militaris with xylitol 2.5 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, standing at 50 deg.C for 10 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid. Each bottle is filled with 100 ml.

Example 2: preparation of oral liquid composition

The formula is as follows: 8 parts of ginseng, 8 parts of cordyceps militaris, 4 parts of prepared rehmannia root, 8 parts of epimedium herb, 1 part of cistanche, 3 parts of cervus elaphus linnaeus, 6 parts of xylitol, water and a proper amount, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 10 g.

The preparation method comprises the following steps: (1) decocting radix Ginseng 8 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum in 5 times of water for 12 hr, decocting for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (3) mixing Cordyceps militaris with xylitol 3 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, standing at 50 deg.C for 8 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid. Each bottle is filled with 100 ml.

Example 3: preparation of oral liquid composition

The formula is as follows: 8 parts of ginseng, 4 parts of cordyceps militaris, 8 parts of prepared rehmannia root, 4 parts of epimedium herb, 3 parts of cistanche, 1 part of cervus elaphus linnaeus, 10 parts of xylitol, water and a proper amount, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 6 g.

The preparation method comprises the following steps: (1) decocting 5 times of radix Ginseng in water for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 8 times of water for 12 hr, decocting for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (3) mixing Cordyceps militaris, 2 times of xylitol and 3 times of water, homogenizing until the mixture can pass through a 120-mesh stainless steel net, standing the slurry at 50 deg.C for 12 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water of formula amount, and stirring; (4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid. Each bottle is filled with 100 ml.

Example 4: preparation of oral liquid composition

The formula is as follows: 8 parts of ginseng, 7 parts of cordyceps militaris, 5 parts of prepared rehmannia root, 7 parts of epimedium herb, 1.5 parts of cistanche, 2.5 parts of cervus elaphus linnaeus, 7 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 9 g.

The preparation method comprises the following steps: (1) decocting 7 times of radix Ginseng in water for 1.1 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 0.9 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 0.6 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 7 times of water for 12 hr, decocting for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.6 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (3) mixing Cordyceps militaris with xylitol 2.8 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, standing at 50 deg.C for 9 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid. Each bottle is filled with 100 ml.

Example 5: preparation of oral liquid composition

The formula is as follows: 8 parts of ginseng, 5 parts of cordyceps militaris, 7 parts of prepared rehmannia root, 5 parts of epimedium herb, 2.5 parts of cistanche, 1.5 parts of cervus elaphus linnaeus, 9 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 7 g.

The preparation method comprises the following steps: (1) decocting radix Ginseng 6 times of water for 1.4 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.6 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.9 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum in 6 times of water for 12 hr, decocting for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 0.8 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 (40 ℃) for later use; (3) mixing Cordyceps militaris with xylitol 2.2 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, standing at 50 deg.C for 11 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of water according to the formula amount, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ under hot pressure for 20 minutes to obtain the composition in the form of oral liquid. Each bottle is filled with 100 ml.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

1. A composition for delaying aging, inhibiting tumor cell growth and improving cardiovascular and cerebrovascular microcirculation, which is prepared from the following substances: 8 parts of ginseng, 4-8 parts of cordyceps militaris, 4-8 parts of prepared rehmannia root, 4-8 parts of epimedium herb, 1-3 parts of cistanche, 1-3 parts of cervus elaphus linnaeus and a physiologically acceptable carrier;

the composition is in the form of oral liquid, the dosage of ginseng in each 100ml of the oral liquid is 6-10 g, and the composition is prepared by the following steps:

(1) decocting 5-8 times of ginseng in water for 1-1.5 hours, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; mixing the filtrates, concentrating to one third of the volume, adding ethanol until the ethanol content reaches 70-75%, mixing uniformly, and standing for 24 hours; taking the supernatant and the sediment filtrate, recovering ethanol, concentrating to obtain a clear paste with the density of 1.25-1.30 measured at 40 ℃, and adding 5-15% of sodium tartrate into the clear paste for later use;

(2) soaking radix rehmanniae preparata, herba epimedii, herba cistanche and cervus elaphus linnaeus in 5-8 times of water for 12 hours, then decocting and extracting for 1-1.5 hours, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; adding 4-6 times of water into filter residues, decocting and extracting for 0.5-1 hour, and filtering to obtain filtrate; mixing the filtrates, concentrating to one third of the volume, adding ethanol until the ethanol content reaches 70-75%, mixing uniformly, and standing for 24 hours; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain clear paste with the density of 1.25-1.30 measured at 40 ℃ for later use;

(4) dissolving 6-10 parts by weight of xylitol in 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid.

2. The composition according to claim 1, which is made from a material selected from the group consisting of: 8 parts of ginseng, 5-7 parts of cordyceps militaris, 5-7 parts of prepared rehmannia root, 5-7 parts of epimedium herb, 1.5-2.5 parts of cistanche, 1.5-2.5 parts of cervus elaphus linnaeus and a physiologically acceptable carrier.

3. The composition according to claim 1, which is made from a material selected from the group consisting of: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus and a physiologically acceptable carrier.

4. The composition according to claim 1, wherein the dosage of ginseng in each 100ml of the oral liquid is 7-9 g.

5. The composition according to claim 1, wherein the dosage of ginseng per 100ml of the oral liquid is 8 g.

6. The composition according to claim 1, wherein the prescribed amount of water is such that the amount of ginseng dosed per 100ml of the oral liquid is 6-10 g.

7. The composition according to claim 1, wherein the prescribed amount of water is such that the amount of ginseng dosed per 100ml of the oral liquid is 7-9 g.

8. The composition according to claim 1, which is prepared from the following formula and preparation method of an oral liquid of any one of composition a1 to composition a 5:

oral liquid of composition a 1:

the formula is as follows: 8 parts of ginseng, 6 parts of cordyceps militaris, 6 parts of prepared rehmannia root, 6 parts of epimedium herb, 2 parts of cistanche, 2 parts of cervus elaphus linnaeus, 8 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 8 g;

the preparation method comprises the following steps: (1) decocting radix Ginseng 6 times of water for 1.25 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediments, recovering ethanol, concentrating to obtain a fluid extract with the density of 1.25-1.30 at 40 ℃, adding sodium tartrate which is 10% of the weight of the fluid extract, and uniformly mixing for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 7 times of water for 12 hr, decocting for 1.25 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; decocting the filter residue with 5 times of water for 0.75 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain a clear paste with a density of 1.25-1.30 at 40 ℃ for later use; (3) mixing Cordyceps militaris with xylitol 2.5 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, placing the pulp at 50 deg.C for 10 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid;

oral liquid of composition a 2:

the formula is as follows: 8 parts of ginseng, 8 parts of cordyceps militaris, 4 parts of prepared rehmannia root, 8 parts of epimedium herb, 1 part of cistanche, 3 parts of cervus elaphus linnaeus, 6 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 10 g;

the preparation method comprises the following steps: (1) decocting radix Ginseng 8 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediments, recovering ethanol, concentrating to obtain a fluid extract with the density of 1.25-1.30 at 40 ℃, adding sodium tartrate which is 15% of the weight of the fluid extract, and uniformly mixing for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum in 5 times of water for 12 hr, decocting for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain a clear paste with a density of 1.25-1.30 at 40 ℃ for later use; (3) mixing Cordyceps militaris with xylitol 3 times and water 3 times, homogenizing until the mixture can pass through 120 mesh stainless steel net, placing the slurry at 50 deg.C for 8 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid;

oral liquid of composition a 3:

the formula is as follows: 8 parts of ginseng, 4 parts of cordyceps militaris, 8 parts of prepared rehmannia root, 4 parts of epimedium herb, 3 parts of cistanche, 1 part of cervus elaphus linnaeus, 10 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 6 g;

the preparation method comprises the following steps: (1) decocting 5 times of radix Ginseng in water for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediments, recovering ethanol, concentrating to obtain a fluid extract with the density of 1.25-1.30 at 40 ℃, adding sodium tartrate which is 5% of the weight of the fluid extract, and uniformly mixing for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 8 times of water for 12 hr, decocting for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.5 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain a clear paste with a density of 1.25-1.30 at 40 ℃ for later use; (3) mixing Cordyceps militaris, 2 times of xylitol and 3 times of water, homogenizing until the mixture can pass through a 120-mesh stainless steel net, placing the slurry at 50 deg.C for 12 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water of formula amount, and stirring; (4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid;

oral liquid of composition a 4:

the formula is as follows: 8 parts of ginseng, 7 parts of cordyceps militaris, 5 parts of prepared rehmannia root, 7 parts of epimedium herb, 1.5 parts of cistanche, 2.5 parts of cervus elaphus linnaeus, 7 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 9 g;

the preparation method comprises the following steps: (1) decocting 7 times of radix Ginseng in water for 1.1 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 0.9 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 0.6 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediments, recovering ethanol, concentrating to obtain a fluid extract with the density of 1.25-1.30 at 40 ℃, adding sodium tartrate accounting for 12% of the weight of the fluid extract, and uniformly mixing for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum with 7 times of water for 12 hr, decocting for 1.5 hr, and filtering to obtain filtrate; decocting the filter residue with 6 times of water for 0.6 hr, and filtering to obtain filtrate; decocting the filter residue with 4.5 times of water for 1 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 75%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain a clear paste with a density of 1.25-1.30 at 40 ℃ for later use; (3) mixing Cordyceps militaris with xylitol 2.8 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, placing the pulp at 50 deg.C for 9 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid;

oral liquid of composition a 5:

the formula is as follows: 8 parts of ginseng, 5 parts of cordyceps militaris, 7 parts of prepared rehmannia root, 5 parts of epimedium herb, 2.5 parts of cistanche, 1.5 parts of cervus elaphus linnaeus, 9 parts of xylitol and a proper amount of water, wherein the feeding amount of the ginseng in each 100ml of the oral liquid is 7 g;

the preparation method comprises the following steps: (1) decocting radix Ginseng 6 times of water for 1.4 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.6 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.9 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the sediment filtrate, recovering ethanol, concentrating to obtain a fluid extract with a density of 1.25-1.30 at 40 ℃, adding sodium tartrate which is 8% of the fluid extract by weight, and uniformly mixing for later use; (2) soaking radix rehmanniae Preparata, herba Epimedii, Cistanchis herba, and cornu Cervi Pantotrichum in 6 times of water for 12 hr, decocting for 1 hr, and filtering to obtain filtrate; decocting the filter residue with 4 times of water for 0.8 hr, and filtering to obtain filtrate; decocting the filter residue with 5.5 times of water for 0.5 hr, and filtering to obtain filtrate; mixing filtrates, concentrating to one third volume, adding ethanol until ethanol content reaches 70%, mixing, and standing for 24 hr; taking the supernatant and the filtrate of the sediment, recovering ethanol, and concentrating to obtain a clear paste with a density of 1.25-1.30 at 40 ℃ for later use; (3) mixing Cordyceps militaris with xylitol 2.2 times and water 3 times, homogenizing until the pulp can pass through 120 mesh stainless steel net, placing the pulp at 50 deg.C for 11 hr, mixing with the fluid extract obtained in step (1) and step (2), dissolving with 30% water, and stirring; (4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid.

9. A process for preparing a composition according to any one of claims 1 to 8 in the form of an oral liquid comprising the steps of:

(4) dissolving xylitol with 20% of formula amount of water, adding the mixed solution obtained in the step (3), stirring to dissolve, adding water to the formula amount, filtering, subpackaging, and sterilizing at 120 ℃ for 20 minutes under hot pressure to obtain the composition in the form of oral liquid.

10. Use of the composition of any one of claims 1 to 8 in the preparation of a product for delaying senescence, inhibiting growth of tumor cells, and improving cardiovascular and cerebrovascular microcirculation.