CN108434174A - It is a kind of to be used to assist dental caries therapeutic and the mouth sprays and preparation method thereof of pre- anti-caries - Google Patents
It is a kind of to be used to assist dental caries therapeutic and the mouth sprays and preparation method thereof of pre- anti-caries Download PDFInfo
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- CN108434174A CN108434174A CN201810367654.6A CN201810367654A CN108434174A CN 108434174 A CN108434174 A CN 108434174A CN 201810367654 A CN201810367654 A CN 201810367654A CN 108434174 A CN108434174 A CN 108434174A
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- caries
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Abstract
The present invention provides a kind of for assisting dental caries therapeutic and the mouth sprays and preparation method thereof of pre- anti-caries.The mouth sprays of the present invention include raw material：Apiolin, lactitol, fanesol, antierythrite, Epigallo-catechin gallate (EGCG), propolis extract, menthol, licochalcone A, ethyl alcohol, sterile water.The mouth sprays of the present invention have apparent antibacterial action, especially there is good antibacterial effect to the main pathogenic bacteria Streptococcus mutans of saprodontia, it can inhibit the growth of Streptococcus mutans etc., and there is good removal ability to the biomembrane of the formation such as Streptococcus mutans, therefore can effectively assist dental caries therapeutic and pre- anti-caries；Simultaneously conducive to re-epithelilization to heal conducive to ulcer wound, there is apparent antibacterial, anti-inflammatory, promote ulcer healing effect.
Description
Technical field
The present invention relates to a kind of for assisting dental caries therapeutic and the mouth sprays and preparation method thereof of pre- anti-caries, belongs to doctor
Medicine technical field.
Background technology
Saprodontia is commonly called as decayed tooth, decayed tooth, is a kind of bacteriosis, can cause pulpitis and periapical inflammation, or even can draw
Play alveolar bone and inflammation of jaws；Not in time such as dental caries therapeutic, lesion continues to develop, and will form cavity in a tooth, is gradually developed to corona
It destroys and disappears completely, eventually lead to loss of tooth.Morbidity of dental caries is high, widely distributed, is one of main common disease in oral cavity, generation
Itself and tumour and angiocardiopathy are listed as three big keypoint control disease of the mankind by boundary's health organization.
Bacterium is the pathogenetic necessary condition of dental caries, and the main pathogenic bacteria of saprodontia is presently believed to be Streptococcus mutans, therefore
Inhibition and removing to Streptococcus mutans are also important a step during prevention and treatment of saprodontia；Dental caries are also
One longer process, removed in time in dental caries generating process pathogenic factor can make saprodontia terminate progress, prevent saprodontia to
Even more serious direction develops or secondary pulpitis, periapical inflammation etc., therefore needs in the treatment of saprodontia and during preventing pair
Cariogenic Streptococcus mutans remove sterilization in time.
The treatment of clinic saprodontia predominantly removes the treatment of dental caries bad tissue augmentation at present, lacks the treatment to saprodontia pathogenic bacteria
Strategy, and it is also less in the drug in pre- anti-caries stage.Chinese patent literature CN105106040A discloses a kind of oral cavity shield
Tooth spray, formula by natural plant essential oils, surfactant, menthol, ethyl alcohol, stevioside, Mint Essence, food coloring,
Sodium citrate, sodium fluoride and deionized water composition.The oral cavity shield tooth spray that the invention provides can prevention of gingivitis, reduction tooth bacterium
Spot eliminates oral peculiar smell, pre- child-resistant decayed tooth saprodontia etc., but the specific aim that the prevention of dental caries is treated in the invention is poor, preventing decayed tooth work(
Effect effect is weaker, and natural active matter is less, and antibacterial, promotes wound healing effect not good enough at anti-inflammatory；And the toxic secondary work of sodium fluoride
With.
Therefore, urgent need is a kind of can effectively inhibit being used for a long time and having no toxic side effect medical for Streptococcus mutans
Product, to increase the control effect to saprodontia.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of for assisting the oral cavity of dental caries therapeutic and pre- anti-caries to spray
Agent.The mouth sprays that the present invention obtains have apparent antibacterial action, especially have to the main pathogenic bacteria Streptococcus mutans of saprodontia
There is good antibacterial effect, can inhibit the growth of Streptococcus mutans etc., and has to the biomembrane of the formation such as Streptococcus mutans good
Good removal ability, therefore can effectively assist dental caries therapeutic and pre- anti-caries；Simultaneously conducive to re-epithelilization to profit
It heals in ulcer wound, there is apparent antibacterial, anti-inflammatory, promote ulcer healing effect.
The present invention also provides a kind of preparation methods for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries.
Technical scheme is as follows：
It is a kind of to be used to that dental caries therapeutic and the mouth sprays of pre- anti-caries, including the raw material of following percentage to be assisted to be made：
The sum of each component is absolutely.
It is described for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including following hundred according to currently preferred
The raw material of ratio is divided to be made：
The sum of each component is absolutely.
It is described for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including following hundred according to currently preferred
The raw material of ratio is divided to be made：
The sum of each component is absolutely.
According to currently preferred, the alloy alcohol is TT- fanesols, i.e. trans-trans fanesol.
According to currently preferred, the menthol is levorotatory menthol.
According to currently preferred, the ethyl alcohol is the edible alcohol that Ti accumulates Nong Du≤95.0%.
According to the present invention, the propolis extract is the resin (natural gum) that honeybee acquires from plant gemma or trunk, is mixed
Enter thereon palatine gland, a kind of colloidal solid object with aromatic odor that the secretion of wax gland is process, complicated component, mainly
Ingredient is flavone compound, terpenoid, organic acid, armaticity aldehydes and a variety of aminoacidases, vitamin, minerals
Deng.It is common to cook nutritional supplement, food and beverage additive.
According to currently preferred, the Epigallo-catechin gallate (EGCG), lactitol, antierythrite, menthol,
Fanesol, apiolin, licochalcone A are the food or pharmaceutical grade of purity >=98%, bee in the propolis extract
Mass content >=40% of glue flavones, propolis extract are food or pharmaceutical grade.
The above-mentioned preparation method for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including step：
(1) lactitol, antierythrite, Epigallo-catechin gallate (EGCG) are dissolved in sterile water, are uniformly mixed
Solution A；
(2) apiolin, fanesol, propolis extract, licochalcone A, menthol are dissolved in ethyl alcohol, are uniformly mixed
Obtain solution B；
(3) solution A and solution B are uniformly mixed, through filtration sterilization, it is filling in spray bottle to obtain the final product.
According to the present invention, the commercially available acquisition of spray bottle described in step (4).
Apiolin (Apigenin) is natural plant extracts in the present invention, has natural antibacterial activity, while having anti-
Tumour, virus, the biological actions such as protection cardiovascular and cerebrovascular；Apiolin of the present invention is preferably food or pharmaceutical grade, commercially available to obtain
.
EGCG is natural plant extracts in the present invention, is natural activity Fungicidal substance, can inhibit Streptococcus mutans growth,
Mitigate the adherency to oral cavity Acquired Pellicle of Streptococcus mutans and actinomyces viscosus, while there is antibacterial, antiviral, anti-oxidant, anti-dynamic
Arteries and veins hardening, antithrombus formation, anti-inflammatory and antitumor action.Food that EGCG of the present invention is preferably purity more than or equal to 98% or
Person's pharmaceutical grade, commercially available acquisition.
Propolis extract has significant curative effect to the oral cavities such as bacterium, fungi, virus pathogeny in the present invention, and normally thin to oral cavity
Born of the same parents are without bad toxic side effect；It can inhibit the glycometabolism system of the pathogens such as Streptococcus mutans；And be conducive to re-epithelilization,
To heal conducive to ulcer wound；Propolis extract of the present invention is preferably high-purity food or pharmaceutical grade, commercially available acquisition.
Fanesol is preferably TT- fanesols in the present invention, can inhibit the generation of the polysaccharide of the pathogenic bacteria such as Streptococcus mutans
Synthesis and the formation of its biomembrane are thanked, to be conducive to the prevention of saprodontia；Fanesol of the present invention is preferably that purity is more than or equal to
98% food or pharmaceutical grade, commercially available acquisition.
Mesoerythrit of the present invention can inhibit Streptococcus mutans, the pathogenic bacteria of decayed tooth such as actinomyces viscosus, and it is formed
Biomembrane has good removal ability, and there is tasty and refreshing pure sweet taste in be not involved in glycometabolism while；Antierythrite of the present invention is excellent
It is selected as high-purity food or pharmaceutical grade, commercially available acquisition.
In the present invention licochalcone A be natural plant extracts, be natural activity Fungicidal substance, have apparent antibacterial,
Anti-inflammatory promotees ulcer healing effect, simultaneous to can be used as fragrance；Licochalcone A of the present invention is preferably food or pharmaceutical grade, commercially available to obtain
.
Lactitol is fragrance in the present invention, while having the function of assisting Antivariant streptococcus, is not involved in bacterium glycometabolism,
Do not cause saprodontia simultaneously；Lactitol of the present invention is preferably high-purity food or pharmaceutical grade, commercially available acquisition.
Menthol is preferably levorotatory menthol in the present invention, is natural plant extracts, has cool pain-alleviating, auxiliary anti-
Inflammation, the effect of pure and fresh mouthfeel；Menthol of the present invention is preferably high-purity food or pharmaceutical grade, commercially available acquisition.
Advantageous effect：
1, the purpose of the invention is to increase the controlling mode in clinical and daily life to saprodontia and improve treatment and
Control effect, and increase the convenience of saprodontia prevention；Saprodontia Susceptible population is helped to prevent saprodontia to more when saprodontia is slighter
For serious direction secondary disease, the increase of pre- anti-caries damage envelope reduces saprodontia after tooth body, endodontic treatment or repairing and treating
Generation；There is certain antiinflammation to oral cavity mucosal inflammation simultaneously, and ulcer healing can be promoted；Reduce family's tendency saprodontia hair
Raw probability.
2, Epigallo-catechin gallate (EGCG), propolis extract, fanesol, apiolin are used as mainly in the present invention
Ingredient, collaboration antierythrite, licochalcone A etc. make mouth sprays prepared by the present invention have excellent antibacterial action, can press down
The growth of the pathogenic bacteria of decayed tooth such as Streptococcus mutans processed, actinomyces viscosus, inhibits the glycometabolism of pathogen, can effectively remove variation chain
The biomembrane that the pathogenic bacteria such as coccus and actinomyces viscosus are formed, and effectively inhibit the formation of above-mentioned pathogenic bacteria biomembrane, to advantageous
In the prevention of saprodontia；The mouth sprays of the present invention are conducive to re-epithelilization, to heal conducive to ulcer wound；Mouth of the present invention
Chamber spray is raw materials used green safe, to human body without bad toxic side effect；The mutual synergistic effect of the various raw material components of the present invention,
The excellent results of the present invention are just reached.
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but not limited to this.
Experimental method described in following embodiments is unless otherwise specified conventional method simultaneously；The reagent and material
Material, unless otherwise specified, commercially obtains.
In following, apiolin, pharmaceutical grade, purity 98%, Shaanxi Sen Fu natural products Co., Ltd is on sale；Lactitol, food
Grade, purity 99%, Henan Qi Nuo food ingredients Co., Ltd are on sale；TT- fanesols, pharmaceutical grade, 99.5%, golden space chemical industry
Company is on sale；Antierythrite, food-grade, purity 99%, Shaanxi Sen Fu natural products Co., Ltd are on sale；Epigallocatechin
Gallate, food-grade, purity 98%, Shaanxi Sen Fu natural products Co., Ltd are on sale；Propolis extract, food-grade, bee
Glue flavones mass content is 40%, and Shaanxi Sen Fu natural products Co., Ltd is on sale；Levorotatory menthol, food-grade, purity 99%,
Shenzhen Ding Cheng plant perfumes company is on sale；Licochalcone A, pharmaceutical grade, purity 98%, Kang Baotai Fine Chemical Co., Ltd have
It sells；Ethyl alcohol is that volumetric concentration is 95% edible alcohol, and it is on sale to neutralize Sheng Tai companies for food-grade.
Embodiment 1
It is a kind of to be used to that dental caries therapeutic and the mouth sprays of pre- anti-caries, including the raw material of following percentage to be assisted to be made：
The sum of each component is absolutely.
The above-mentioned preparation method for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including step：
(1) 2g lactitols, 1g antierythrites, 2g EGCG are weighed respectively is dissolved in wiring solution-forming in 500g sterile waters, mixing,
Obtain solution A；
(2) it is left-handed that 1g apiolins, 2g TT- fanesols, 3g propolis extracts, 1g licochalcone As, 3g are weighed respectively
Menthol is dissolved in the edible alcohol that 50g volumetric concentrations are 95%, and mixing obtains solution B；
(3) solution A and solution B are uniformly mixed, addition sterile water is to 1000g, filling to be in spray bottle through filtration sterilization
.
Embodiment 2
It is a kind of to be used to that dental caries therapeutic and the mouth sprays of pre- anti-caries, including the raw material of following percentage to be assisted to be made：
The sum of each component is absolutely.
The above-mentioned preparation method for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including step：
(1) 1g lactitols, 0.5g antierythrites, 1g EGCG are weighed respectively and is dissolved in wiring solution-forming in 500g sterile waters, are mixed
It is even, obtain solution A；
(2) 0.5g apiolins, 1g TT- fanesols, 2g propolis extracts, 0.5g licochalcone As, 2g are weighed respectively
Levorotatory menthol is dissolved in the edible alcohol that 20g volumetric concentrations are 95%, and mixing obtains solution B；
(3) solution A and solution B are uniformly mixed, addition sterile water is to 1000g, filling to be in spray bottle through filtration sterilization
.
Embodiment 3
It is a kind of to be used to that dental caries therapeutic and the mouth sprays of pre- anti-caries, including the raw material of following percentage to be assisted to be made：
The sum of each component is absolutely.
The above-mentioned preparation method for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, including step：
(1) 3g lactitols, 2g antierythrites, 3g EGCG are weighed respectively is dissolved in wiring solution-forming in 500ml sterile waters, mixing,
Obtain solution A；
(2) it is left-handed that 2g apiolins, 3g TT- fanesols, 4g propolis extracts, 2g licochalcone As, 4g are weighed respectively
Menthol is dissolved in the edible alcohol that 80g volumetric concentrations are 95%, and mixing obtains solution B；
(3) solution A and solution B are uniformly mixed, addition sterile water is to 1000g, filling to be in spray bottle through filtration sterilization
.
Comparative example 1
A kind of mouth sprays, raw material composition is as described in Example 1, except that：Without apiolin in component, it is other with it is real
It is consistent to apply example 1.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Comparative example 2
A kind of mouth sprays, raw material composition is as described in Example 1, except that：Without TT- fanesols in component,
It is consistent with embodiment 1.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Comparative example 3
A kind of mouth sprays, raw material composition is as described in Example 1, except that：Without antierythrite, Qi Tayu in component
Embodiment 1 is consistent.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Comparative example 4
A kind of mouth sprays, raw material composition is as described in Example 1, except that：Without epigallocatechin in component
Gallate, it is other consistent with embodiment 1.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Comparative example 5
A kind of mouth sprays, raw material composition is as described in Example 1, except that：It is other without propolis extract in component
It is consistent with embodiment 1.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Comparative example 6
A kind of mouth sprays, raw material composition is as described in Example 1, except that：Without licochalcone A in component,
It is consistent with embodiment 1.
The preparation method of above-mentioned mouth sprays is consistent with embodiment 1.
Test example 1
To the measurement of Streptococcus mutans minimal inhibitory concentration MIC and minimum bactericidal concentration MBC
1, the preparation of sample to be tested
The mouth sprays spraying with sterile water mixed preparing various concentration respectively prepared by embodiment 1 and comparative example 1-6
Agent solution (8000,4000,2000,1000,500,250,125,62.5,32.15,15.625,7.8,3.9ug/ml), as waiting for
Sample；The computational methods of sample to be tested concentration are：The volume of the density * mouth sprays of mouth sprays/(mouth sprays and sterile
The total volume of water), different mouth sprays density are each about 1mg/ml.
Simultaneously respectively prepare various concentration (8000,4000,2000,1000,500,250,125,62.5,32.15,
15.625,7.8,3.9ug/ml) apiolin ethanol water (mass concentration of ethyl alcohol is 10% in ethanol water), EGCG
Aqueous solution, the aqueous solution of antierythrite, propolis extract ethanol water (mass concentration of ethyl alcohol is in ethanol water
10%), the ethanol water (mass concentration of ethyl alcohol is 10% in ethanol water) of TT- fanesols, as sample to be tested.
2, test method
Above-mentioned sample to be tested is separately added into 96 porocyte culture plates of sterilizing；By Streptococcus mutans turbid liquid concentration tune
To 0.5 Maxwell than turbid, after 1000 times of BHI broth dilutions, 100ul is added into above-mentioned every hole, 18- are cultivated in 37 DEG C after sealing
It is observed afterwards for 24 hours；Lowest concentration of drug to completely inhibit bacterial growth in hole is MIC；Continue to observe bacterium after cultivating 48h
Growing state, applies tablet observation, and asepsis growth hole contained drug minimum concentration is MBC.Test result is as follows shown in table：
1 minimal inhibitory concentration MIC of table and minimum bactericidal concentration MBC test datas
Conclusion：Mouth sprays prepared by the present invention, which have Streptococcus mutans compared with comparative example and one-component, preferably to kill
Bacterium sterilization effect illustrates to play synergistic effect between each raw material to reach excellent anti-microbial property.
Test example 2
Influence to biofilm formation
1, the preparation of sample to be tested
It is 1 institute of test example that mouth sprays and sterile water prepared by embodiment 1 and comparative example 1-6, which are mixed compound concentration respectively,
The spray solution for obtaining minimum bactericidal concentration, as sample to be tested；
Apiolin ethanol water (the ethanol water of difference compound concentration 2mg/ml (sufficiently achieving minimum bactericidal concentration) simultaneously
In solution the mass concentration of ethyl alcohol be 10%), the ethanol water of the aqueous solution of the aqueous solution of EGCG, antierythrite, propolis extract
Ethanol water (the second in ethanol water of solution (in ethanol water the mass concentration of ethyl alcohol be 10%), TT- fanesols
The mass concentration of alcohol is 10%), as sample to be tested.
2, test method
It is 0.2 by the BHI broth dilutions to 655nm absorbances of the Streptococcus mutans after culture for 24 hours；Take 96 holes of sterilizing thin
Born of the same parents' culture plate is separately added into sample to be tested and the above-mentioned bacterium solutions of 100ul into plate hole, after 37 DEG C are cultivated 48h, absorbs culture solution and hangs
Floating bacterium leaves behind biomembrane in hole, sterile distilled water is used in combination to rinse biomembrane 3 times, state then up in hole be separately added into it is dense
The crystal violet that degree is 0.4wt% dyes 15min to biomembrane；The unbonded crystal violet of 4 removings of aseptic water washing, 37 DEG C of dryings
2h；To above-mentioned per being added the ethyl alcohol that 100ul volumetric concentrations are 95% in hole, rocker 10min by the pigment in biomembrane discharge to
In ethyl alcohol, the absorbance of ethyl alcohol is tested；Control group is set, water is only added in the hole of control group, absorbance is as 100%.Test
The results are shown in table below：
Influence of the different samples of table 2 to biofilm formation
Conclusion：Although the mouth sprays in each independent component and comparative example can also inhibit the hammer that makes a variation under to a certain degree
Bacterium forms biomembrane, but obviously streptococcus mutans biomembrane is formed not as good as the mouth sprays of the preparation of the embodiment of the present invention 1
Inhibition is apparent, illustrates that the shape of streptococcus mutans biomembrane could effectively be inhibited by playing synergistic effect between each raw material of the present invention
At.
Test example 3
To the ability of pathogenic bacteria biomembrane removal
1, the preparation of sample to be tested
It is 1 institute of test example that mouth sprays and sterile water prepared by embodiment 1 and comparative example 1-6, which are mixed compound concentration respectively,
The spray solution for obtaining minimum bactericidal concentration, as sample to be tested；
Apiolin ethanol water (the ethyl alcohol that compound concentration is 2mg/ml (sufficiently achieving minimum bactericidal concentration) respectively simultaneously
In aqueous solution the mass concentration of ethyl alcohol be 10%), the ethyl alcohol of the aqueous solution of the aqueous solution of EGCG, antierythrite, propolis extract
The ethanol water (in ethanol water of aqueous solution (in ethanol water the mass concentration of ethyl alcohol be 10%), TT- fanesols
The mass concentration of ethyl alcohol is 10%), as sample to be tested.
2, test method
It is 0.2 by the BHI broth dilutions to 655nm absorbances of the Streptococcus mutans after culture for 24 hours, and 100ul is taken to distinguish
It is added in the hole of 96 porocyte culture plates, 37 DEG C of culture 48h to biofilm formation；It is separately added into sample to be tested in above-mentioned hole, 37
After DEG C Anaerobic culturel 20min, culture solution and suspended bacterial are absorbed, biomembrane is left behind in hole, three times with aseptic water washing, Kong Zhong
A concentration of 0.4wt% of biomembrane violet staining 15min, it is dry then with aseptic water washing 4 times；Then add to every hole
It is 95% ethyl alcohol to enter 100ul volumetric concentrations, and rocker 10min discharges the pigment in biomembrane into ethyl alcohol, tests the suction of ethyl alcohol
Luminosity；Control group is set, water is only added in the hole of control group, absorbance is as 100%.Test result is as follows shown in table：
Test data of the different samples of table 3 to biomembrane removal ability
Conclusion：Although each component and the mouth sprays of comparative example can also remove established streptococcus mutans biomembrane,
But the mouth sprays with obvious effects prepared not as good as the present invention illustrate that each raw material component of the present invention plays synergistic effect to shape
At the excellent removal ability that has of streptococcus mutans biomembrane.
Claims (8)
1. a kind of for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that include the original of following percentage
Material is made：
The sum of each component is absolutely.
2. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that including
The raw material of following percentage is made：
The sum of each component is absolutely.
3. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that including
The raw material of following percentage is made：
The sum of each component is absolutely.
4. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that described
Alloy alcohol is TT- fanesols.
5. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that described
Menthol is levorotatory menthol.
6. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that described
Ethyl alcohol is the edible alcohol that Ti accumulates Nong Du≤95.0%.
7. according to claim 1 for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries, which is characterized in that described
Epigallo-catechin gallate (EGCG), lactitol, antierythrite, menthol, fanesol, apiolin, licochalcone A are equal
For the food or pharmaceutical grade of purity >=98%, mass content >=40% of propolis flavone, propolis carry in the propolis extract
It is food or pharmaceutical grade to take object.
8. as claim 1-7 any one of them is used to assist the preparation side of dental caries therapeutic and the mouth sprays of pre- anti-caries
Method, including step：
(1) lactitol, antierythrite, Epigallo-catechin gallate (EGCG) are dissolved in sterile water, are uniformly mixed to obtain solution
A；
(2) apiolin, fanesol, propolis extract, licochalcone A, menthol are dissolved in ethyl alcohol, are uniformly mixed molten
Liquid B；
(3) solution A and solution B are uniformly mixed, through filtration sterilization, it is filling in spray bottle to obtain the final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810367654.6A CN108434174B (en) | 2018-04-23 | 2018-04-23 | It is a kind of for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810367654.6A CN108434174B (en) | 2018-04-23 | 2018-04-23 | It is a kind of for assisting dental caries therapeutic and the mouth sprays of pre- anti-caries and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115350098A (en) * | 2022-07-28 | 2022-11-18 | 南方科技大学 | Ready-to-use polyacrylic acid silver fluoride anti-caries coating paste and using method and tool |
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| CN115350098A (en) * | 2022-07-28 | 2022-11-18 | 南方科技大学 | Ready-to-use polyacrylic acid silver fluoride anti-caries coating paste and using method and tool |
| CN115350098B (en) * | 2022-07-28 | 2023-12-15 | 南方科技大学 | Instant silver polyacrylate fluoride caries-preventing paste, using method and tool |
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| CN108434174B (en) | 2019-11-22 |
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