CN108348556A - 细胞周期阻滞提高产生诱导多能干细胞的效率 - Google Patents

细胞周期阻滞提高产生诱导多能干细胞的效率 Download PDF

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CN108348556A
CN108348556A CN201680063137.9A CN201680063137A CN108348556A CN 108348556 A CN108348556 A CN 108348556A CN 201680063137 A CN201680063137 A CN 201680063137A CN 108348556 A CN108348556 A CN 108348556A
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cell
phases
cell cycle
inhibitor
cycle
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R·Y·史密斯
M·A·格利克斯曼
N·F·扎伊迪
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European 3 Co
Seaport Diagnostics Inc
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European 3 Co
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
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    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2510/00Genetically modified cells

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CN201680063137.9A 2015-11-02 2016-10-27 细胞周期阻滞提高产生诱导多能干细胞的效率 Pending CN108348556A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562249520P 2015-11-02 2015-11-02
US62/249,520 2015-11-02
PCT/US2016/059112 WO2017079029A1 (en) 2015-11-02 2016-10-27 Cell cycle block improves efficiency in generating induced pluripotent stem cells

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CN108348556A true CN108348556A (zh) 2018-07-31

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EP (1) EP3370744A4 (ja)
JP (1) JP2018531610A (ja)
KR (1) KR20180072817A (ja)
CN (1) CN108348556A (ja)
IL (1) IL258640A (ja)
WO (1) WO2017079029A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115531382A (zh) * 2021-06-29 2022-12-30 中国科学院生物物理研究所 喹啉噻唑啉酮衍生物Ro-3306在治疗β-冠状病毒感染中的应用
WO2023273882A1 (zh) * 2021-06-30 2023-01-05 呈诺再生医学科技(珠海横琴新区)有限公司 一种高效无遗传修饰的iPSC诱导、产业化单克隆挑取平台及应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107589002B (zh) * 2017-09-08 2020-05-29 杭州市妇产科医院 一种诱导多能干细胞染色体的制备方法
US20220267736A1 (en) * 2017-12-28 2022-08-25 Kaneka Corporation Pluripotent stem cell aggregation suppressor
CN110016460A (zh) * 2018-01-09 2019-07-16 中国农业大学 一种高效鸡胚胎原代成纤维细胞g2/m期同步化方法
CN108531447B (zh) * 2018-04-13 2021-11-23 上海市生物医药技术研究院 调节精子运动能力及辅助生殖的化合物及其用途
WO2023243679A1 (ja) * 2022-06-17 2023-12-21 学校法人日本医科大学 高力価ウイルスベクターの製造方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033847A (en) * 1995-02-06 2000-03-07 St. Jude Children's Research Hospital InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof
US20140056860A1 (en) * 2011-04-08 2014-02-27 Universite Montpellier Ii Method for rejuvenating cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032847A2 (en) * 1997-01-23 1998-07-30 The Johns Hopkins University School Of Medicine Characterization of the yeast transcriptome
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9238797B2 (en) * 2008-07-07 2016-01-19 Takara Bio Inc. Method for production of pluripotent stem cell
US20120076762A1 (en) * 2009-03-25 2012-03-29 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation
JP2013510576A (ja) * 2009-11-11 2013-03-28 サンフォード−バーンハム メディカル リサーチ インスティテュート iPS細胞の生成および調節のための方法およびその組成物
CN104278008B (zh) * 2013-07-12 2020-08-21 北京宏冠再生医学科技有限公司 一种通过小分子化合物处理来制备多潜能干细胞的方法、试剂盒和用途
JP6232907B2 (ja) * 2013-10-09 2017-11-22 富士レビオ株式会社 融合細胞およびその作製方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033847A (en) * 1995-02-06 2000-03-07 St. Jude Children's Research Hospital InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof
US20140056860A1 (en) * 2011-04-08 2014-02-27 Universite Montpellier Ii Method for rejuvenating cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHRISTOPH SCHORL等: "Analysis of cell cycle phases and progression in cultured mammalian cells", 《METHODS》 *
R W ALLEN等: "Arrest of cell growth in the G1 phase of the cell cycle by serine deprivation", 《EXPERIMENTAL CELL RESEARCH》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115531382A (zh) * 2021-06-29 2022-12-30 中国科学院生物物理研究所 喹啉噻唑啉酮衍生物Ro-3306在治疗β-冠状病毒感染中的应用
CN115531382B (zh) * 2021-06-29 2024-01-30 中国科学院生物物理研究所 喹啉噻唑啉酮衍生物Ro-3306在治疗β-冠状病毒感染中的应用
WO2023273882A1 (zh) * 2021-06-30 2023-01-05 呈诺再生医学科技(珠海横琴新区)有限公司 一种高效无遗传修饰的iPSC诱导、产业化单克隆挑取平台及应用

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JP2018531610A (ja) 2018-11-01
KR20180072817A (ko) 2018-06-29
EP3370744A1 (en) 2018-09-12
WO2017079029A1 (en) 2017-05-11
IL258640A (en) 2018-06-28
EP3370744A4 (en) 2019-04-17

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