CN108293595A - Utilize the method for the cultivating bisporous mushroom of ginkgo waste - Google Patents
Utilize the method for the cultivating bisporous mushroom of ginkgo waste Download PDFInfo
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- CN108293595A CN108293595A CN201710988387.XA CN201710988387A CN108293595A CN 108293595 A CN108293595 A CN 108293595A CN 201710988387 A CN201710988387 A CN 201710988387A CN 108293595 A CN108293595 A CN 108293595A
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- Prior art keywords
- ginkgo
- extract
- alcohol
- mulberry
- mushroom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The method that the present invention relates to the use of the cultivating bisporous mushroom of ginkgo waste, by ginkgo filter residue 60~75%, mulberry filter residue 40~55%, yellow ginger waste 20~30%, sucrose 0.3~0.6%, urea 0.5~1.5% adds 3~10% straw decomposing inoculants, fully mixes thoroughly, air humidity under the conditions of being 60%~85% heap decomposed 15~40 days, matrix is laid into 5cm or more after again will be decomposed, and sprinkling 5%~15% agaricus bisporus zymotic fluid of inoculation obtains agaricus bisporus through mushroom producing culture.The present invention takes full advantage of ginkgo, mulberry functional components, is conducive to the blood circulation-promoting health function of enhancing agaricus bisporus, edible convenient for various crowds, adds less investment, is at low cost convenient for large-scale production.
Description
Technical field
The method that the present invention relates to the use of the cultivating bisporous mushroom of ginkgo waste belongs to mushroom-cultivating technical field.
Background technology
Agaricus bisporus is also known as white mushroom, mushroom, mushroom, and European and American countries operator is often referred to as common cultivation mushroom
Or button mushroom.Agaricus bisporus is the mushroom class of worldwide cultivation and consumption, there is the title of " world mushroom ", can marketing fresh, canning, salt marsh.
The mycelia of agaricus bisporus is also as the raw material of pharmacy.Agaricus bisporus fructification is medium big, the wide 5-12cm of cap, first hemispherical, after
It is open and flat, white, smooth, slightly dry gradual change yellow, the curls inward of edge initial stage.Bacterial context white, it is thick, slightly become pale red after wound, tool mushroom is special
Some smells.Lamella is just pink, rear change brown to dark brown, close, narrow, from life, Length discrepancy, stem long 4.5-9cm, thick 1.5-
3.5cm, white is smooth, has mercerising, close cylindrical, internal soft or middle reality, collarium single layer, white, film quality, in the middle part of raw stem,
It is easy to fall off.Raw forest land, meadow, field, park, road side etc., distributed pole is extensive, and China generally cultivates.Chinese agaricus bisporus is planted
Training it is most have the provinces such as Fujian, Shandong, Henan, Zhejiang.Planting type has mushroom house cultivation, the cultivation of greenhouse posture and greenhouse ridge cultivation
Deng.Different regions, Different climate condition and Various Seasonal, which can be taken, is suitble to the planting type of oneself.
Existing research shows that containing about protein 3.7g, fat 0.2g, sugar 3.0g, fibre in the every 100g fresh goods of agaricus bisporus
Tie up element 0.8g, phosphorus 110mg, calcium 9mg, iron 0.6mg.Agaricus bisporus is containing there are many amino acid, nucleotide, vitamin B1, vitamins
B2, vitamin C, nicotinic acid, provitamin D etc..Junket ammonia enzyme contained by agaricus bisporus is substantially reduced blood pressure effect, polysaccharide
Quinones is combined with sulfydryl, can inhibit Synthesis of DNA, medically, there is the work for inhibiting activity of tumor cells
With.
Ginkgo is Ginkgoaceae, Ginkgo deciduous tree.Ginkgo is fallen leaves megaphanerophyte, and for the diameter of a cross-section of a tree trunk 1.3 meters above the ground up to 4 meters, treelet bark is closely flat
Sliding, light grey, the skin taupe of tree, irregular lobe are coarse greatly；Have long shoot with it is slow-growing away from shape brachyplast.Childhood and strong
Year tree crown cone, it is always then wide oval；Branch is close verticillate, tiltedly upper stretching, extension.Leaf alternate, it is radial scattered on long shoot, have elongated
Petiole, fan-shaped, two sides light green is hairless.It is scattered on long shoot, the fasciation on brachyplast.Its vein form is " two discrimination shape bifurcateds
Vein ".Normal 2 split on long shoot, base portion wide wedge shape.Globe daisy dioecism, unisexuality are born in the lepidophyllous armpit on brachyplast top
It is interior, it is in fasciation shape.April blooms, and October is ripe, the long stalk of seed tool, sagging, normal for ellipse, long inverted ovoid, oval or nearly circle
It is spherical.Kind skin and flesh matter, by white powder, exosper meat, yellow or orange-yellow when ripe.
Its ingredient is analyzed from ginkgo leaf extraction essence, finds flavonoids, biflavone body, bilobalide-like in ginkgo leaf
Close the ingredients such as object, can activated blood platelet function, so that blood is not condensed into blocks, it can distend the blood vessels simultaneously promotion artery with it is quiet
The blood circulation of arteries and veins, therefore can prevention of cardiovascular disease, cerebral thrombus and apoplexy.It is also good ornamental plant, plantation at present is advised
Mould reaches tens of thousands of mus, forms a large amount of Caulis Ginkgo branches and leaves the like waste in the course of cultivation.It is cultivating bisporous to develop ginkgo waste
Mushroom technology can reduce cultivation cost, realize recycling for ginkgo waste, promote increasing peasant income.
Invention content
Being suitable for the agaricus bisporus bacterial strain of ginkgo matrix the purpose of the present invention is selection and breeding are a kind of, and develops mating with ginkgo
For matrix formulations and method that the parent species of major ingredient are prepared and cultivated on a large scale, recycling for ginkgo waste is realized.
The present invention provides the method using the cultivating bisporous mushroom of ginkgo waste, specifically includes that steps are as follows：
(1) ginkgo crushed after being dried is crossed into 20 mesh sieve, 70~85% ethanol solutions is added, extract 3~5h, wherein ethyl alcohol
Volumetric usage L/ ginkgo weight kg is 1:2~5, ginkgo filter residue and ginkgo extract are filtered to obtain, and ginkgo extract is concentrated back
It receives ethyl alcohol to no alcohol taste and obtains ginkgo alcohol extract.
(2) ramulus mori crushed after being dried is crossed into 20 mesh sieve, 70~85% ethanol solutions is added, extract 3~5h, wherein ethyl alcohol
Volumetric usage L/ ramulus mori weight kg is 1:2~5, filter to obtain mulberry filter residue and mulberry extracting solution, and by mulberry extracting solution concentration and recovery ethyl alcohol
Obtain mulberry alcohol extract to no alcohol taste, with ginkgo alcohol extract mix in proportion alcohol extract is spare.
(3) yellow ginger is cleaned, crushed after being dried cross 20 mesh sieve, be added 70~85% ethanol solutions, extract 3~5h,
The wherein volumetric usage L/ yellow ginger weight kg of ethyl alcohol is 1:2~5, filtering removal extracting solution obtains yellow ginger filter residue.
(4) by ginkgo filter residue 60~75%, mulberry filter residue 40~55%, yellow ginger waste 20~30%, sucrose 0.3~
0.6%, urea 0.5~1.5% adds 3~10% straw decomposing inoculants, fully mixes thoroughly, and air humidity is 60%~85%
It carries out heaping decomposed 15~40 days under part, then decomposed rear matrix is laid into 5cm or more, sprinkling 5%~15% pair of spore mushroom of inoculation
Mushroom zymotic fluid.
(5) under the conditions of temperature is 20 DEG C~25 DEG C, air humidity is 40%~55%, it is put into the bacterium germination sterilized in advance
Room, is protected from light culture mycelia 20 days~30 days, alcohol extract is sprayed in matrix immediately, then cover the loess of 3~5cm thickness, in temperature
20 DEG C~25 DEG C, cultivate 4~7 days under the conditions of humidity 85%~95%, ventilation 1 time daily.
(6) harvesting the previous day stops water spray, after harvesting first batch of mushroom, removes remaining mushroom root on cultivating bag, then will be packed
Matrix be placed on 20 DEG C~25 DEG C, under the conditions of air humidity 60%~75% culture mycelia 3~4 days to purseful.
(7) alcohol extract is sprayed again, is cultivated under condition of culture under the conditions of 20 DEG C~25 DEG C, air humidity 85%~95%
10~16 days, ventilation 1 time daily harvested second batch of mushroom.
In a specific embodiment, mulberry alcohol extract described in the step (2) and ginkgo alcohol extract volume ratio are 1：2
~4.
In a specific embodiment, agaricus bisporus vaccination ways described in the step (4) are directly by double spore mushrooms
Mushroom seed liquor is directly sprayed onto in the matrix handled well, and routinely potato fluid nutrient medium carries out inoculation pair to mushroom seed liquor
Spore mushroom is cultivated 3~5 days.
In a specific embodiment, 5%~15% agaricus bisporus zymotic fluid described in the step (4) refers to being added
Fermentating liquid volume L and matrix weight kg ratios.
In a specific embodiment, the weight ratio of the volume and matrix of alcohol extract is sprayed described in the step (5)
For 80~200mL：1kg.
In a specific embodiment, the weight ratio of the volume and matrix of alcohol extract is sprayed described in the step (7)
For 40~80mL：1kg.
In a specific embodiment, the filtering refers to that secondary using three layers of filtered through gauze, ceramic membrane filter is primary,
In 0.2 μm of pore size ceramic membrane filter.
In a specific embodiment, the straw decomposing inoculant contains bacillus subtilis, trichoderma, lactic acid bacteria, lichens
Bacillus one of which is several, can also can voluntarily be prepared from market purchasing, and bacillus subtilis, trichoderma are pressed 1：3
Or 1：5 ratio mixing.
Technique effect
1, the starch and cellulose in existing yellow ginger waste are taken full advantage of, while waste being made to be sent out by agaricus bisporus
After ferment, matrix, which returns to, is used as organic fertilizer in soil, can quickly be utilized, improve yellow ginger waste utilization rate.
2, mulberry, gingko functional ingredient are taken full advantage of, is conducive to the blood circulation-promoting health function of enhancing agaricus bisporus, convenient for each
Kind crowd is edible, adds less investment, is at low cost convenient for large-scale production.
Specific implementation mode
The present invention is further explained in the light of specific embodiments, but its any for being not limited to these examples
A or similar example.
Embodiment 1
Ginkgo crushed after being dried is crossed into 20 mesh sieve, takes 150kg that 80% ethanol solution 450L is added, extracts 4h, filter silver-colored
Apricot filter residue and ginkgo extract, and ginkgo extract concentration and recovery ethyl alcohol to no alcohol taste is obtained into ginkgo alcohol extract.Ramulus mori is carried out
Clean, crushed after being dried crosses 20 mesh sieve, takes 150kg that 80% ethanol solution 450L is added, and extracts 4h, filters to obtain mulberry filter residue and Sang Ti
Liquid is taken, and mulberry extracting solution concentration and recovery ethyl alcohol to no alcohol taste is obtained into mulberry alcohol extract, alcohol extracting is mixed to obtain in proportion with ginkgo alcohol extract
Object is spare.Yellow ginger is cleaned, crushed after being dried cross 20 mesh sieve, take 100kg be added 80% ethanol solution 400L, extract 4h,
Filtering removal extracting solution obtains yellow ginger filter residue.
Ginkgo filter residue 210kg, mulberry filter residue 120kg, yellow ginger waste 60kg, sucrose 1.2kg, urea 1.5kg are taken, is added
16kg straw decomposing inoculants (4% or so), are fully mixed thoroughly, under the conditions of air humidity is 75% heap decomposed 30 days, then will
Matrix is laid into 5cm or more, sprinkling inoculation 32L agaricus bisporus zymotic fluid (8%) after decomposed.It is 22 DEG C, air humidity in temperature
Under the conditions of 65%, it is put into the bacterium germination room sterilized in advance, is protected from light culture mycelia 25 days, sprays alcohol extract in matrix immediately, then
The loess for covering 4cm thickness, is cultivated 6 days under the conditions of 22 DEG C of temperature, humidity 85%, daily ventilation 1 time.Harvest the previous day stopping spray
Water after harvesting first batch of mushroom, removes remaining mushroom root on cultivating bag, then packed matrix is placed on 23 DEG C, air humidity 65%
Mycelia 3~4 days is cultivated under part to purseful.Alcohol extract is sprayed again, and item is cultivated under the conditions of 23 DEG C, air humidity 85%~95%
It is cultivated 14 days under part, daily ventilation 1 time, harvests second batch of mushroom.
Embodiment 2
Ginkgo crushed after being dried is crossed into 20 mesh sieve, takes 200kg that 75% ethanol solution 650L is added, extracts 3.5h, filter
Ginkgo filter residue and ginkgo extract, and ginkgo extract concentration and recovery ethyl alcohol to no alcohol taste is obtained into ginkgo alcohol extract.By ramulus mori into
Row clean, crushed after being dried cross 20 mesh sieve, take 200kg be added 75% ethanol solution 650L, extract 3.5h, filter mulberry filter residue and
Mulberry extracting solution, and mulberry extracting solution concentration and recovery ethyl alcohol to no alcohol taste is obtained into mulberry alcohol extract, it is mixed in proportion with ginkgo alcohol extract
Alcohol extract is spare.Yellow ginger is cleaned, crushed after being dried cross 20 mesh sieve, take 200kg be added 75% ethanol solution 700L, extraction
3.5h, filtering removal extracting solution obtain yellow ginger filter residue.
Ginkgo filter residue 280kg, yellow ginger waste 90kg, mulberry filter residue 180kg, sucrose 2kg, urea 2.5kg are taken, is added
28kg straw decomposing inoculants, are fully mixed thoroughly, under the conditions of air humidity is 70% heap decomposed 40 days, then will decomposed rear matrix
It is laid into 5cm or more, sprinkling inoculation 45L agaricus bisporus zymotic fluids.Under the conditions of temperature is 23 DEG C, air humidity is 65%, put
Enter the bacterium germination room sterilized in advance, be protected from light culture mycelia 28 days, sprays alcohol extract in matrix immediately, then cover the Huang of 3~5cm thickness
Soil is cultivated 7 days under the conditions of 22 DEG C of temperature, humidity 85%, daily ventilation 1 time.The previous day stopping water spray being harvested, harvests first batch
After mushroom, remaining mushroom root on cultivating bag is removed, then packed matrix is placed on 22 DEG C, cultivates mycelia 3 under the conditions of air humidity 70%
~4 days to purseful.Alcohol extract is sprayed again, is cultivated 13 days under condition of culture under the conditions of 22 DEG C, air humidity 85%, it is logical daily
Wind 1 time harvests second batch of mushroom.
Claims (5)
1. using the method for the cultivating bisporous mushroom of ginkgo waste, specifically include that steps are as follows：
(1) ginkgo crushed after being dried is crossed into 20 mesh sieve, 70~85% ethanol solutions is added, extract 3~5h, the wherein volume of ethyl alcohol
Dosage L/ ginkgo weight kg is 1:2~5, filter to obtain ginkgo filter residue and ginkgo extract, and by ginkgo extract concentration and recovery second
Alcohol to no alcohol taste obtains ginkgo alcohol extract；
(2) ramulus mori crushed after being dried is crossed into 20 mesh sieve, 70~85% ethanol solutions is added, extract 3~5h, the wherein volume of ethyl alcohol
Dosage L/ ramulus mori weight kg is 1:2~5, filter to obtain mulberry filter residue and mulberry extracting solution, and by mulberry extracting solution concentration and recovery ethyl alcohol to nothing
Alcohol taste obtains mulberry alcohol extract, with ginkgo alcohol extract mix in proportion alcohol extract is spare；
(3) yellow ginger is cleaned, crushed after being dried cross 20 mesh sieve, be added 70~85% ethanol solutions, extract 3~5h, wherein
The volumetric usage L/ yellow ginger weight kg of ethyl alcohol is 1:2~5, filtering removal extracting solution obtains yellow ginger filter residue；
(4) by ginkgo filter residue 60~75%, mulberry filter residue 40~55%, yellow ginger waste 20~30%, sucrose 0.3~0.6%, urine
Element 0.5~1.5%, adds 3~10% straw decomposing inoculants, fully mixes thoroughly, and air humidity carries out under the conditions of being 60%~85%
It heaps decomposed 15~40 days, then decomposed rear matrix is laid into 5cm or more, sprinkling 5%~15% agaricus bisporus zymotic fluid of inoculation；
(5) under the conditions of temperature is 20 DEG C~25 DEG C, air humidity is 40%~55%, it is put into the bacterium germination room sterilized in advance, is kept away
Optical culture mycelia 20 days~30 days, sprays alcohol extract, then cover the loess of 3~5cm thickness in matrix immediately, 20 DEG C of temperature~
25 DEG C, cultivate 4~7 days under the conditions of humidity 85%~95%, ventilation 1 time daily；
(6) harvesting the previous day stops water spray, after harvesting first batch of mushroom, removes remaining mushroom root on cultivating bag, then by packed matrix
Be placed on 20 DEG C~25 DEG C, under the conditions of air humidity 60%~75% culture mycelia 3~4 days to purseful；
(7) spray alcohol extract again, under the conditions of 20 DEG C~25 DEG C, air humidity 85%~95% under condition of culture culture 10~
16 days, ventilation 1 time daily harvested second batch of mushroom.
2. the method for claim 1 wherein step (2) the mulberry alcohol extract and ginkgo alcohol extract volume ratio are 1：2~4.
3. the method for claim 1 wherein step (5) it is described sprinkling alcohol extract volume and matrix weight ratio be 80~
200mL：1kg.
4. the method for claim 1 wherein step (7) it is described sprinkling alcohol extract volume and matrix weight ratio be 40~
80mL：1kg.
5. the method for claim 1 wherein filtering refers to, secondary using three layers of filtered through gauze, ceramic membrane filter is primary, wherein 0.2 μm
The ceramic membrane filter of pore size.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326487A (en) * | 2019-08-06 | 2019-10-15 | 程亚龙 | Composition and its application containing ramulus mori |
| CN117837436A (en) * | 2024-03-04 | 2024-04-09 | 中国科学院昆明植物研究所 | Wild planting method for boletus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN117837436A (en) * | 2024-03-04 | 2024-04-09 | 中国科学院昆明植物研究所 | Wild planting method for boletus |
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Application publication date: 20180720 |