CN108251377A - 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 - Google Patents
利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 Download PDFInfo
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- CN108251377A CN108251377A CN201810041514.XA CN201810041514A CN108251377A CN 108251377 A CN108251377 A CN 108251377A CN 201810041514 A CN201810041514 A CN 201810041514A CN 108251377 A CN108251377 A CN 108251377A
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0656—Adult fibroblasts
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N2510/00—Genetically modified cells
Abstract
Description
Component | 1× | 20× |
SYBR(2×) | 10μL | 200μL |
cDNA | 1μL | 20μL |
Nuclear-Free water | 4μL | 80μL |
15μL |
Component | 1× | 9× |
Primer F+R(10μM) | 0.8μL | 7.2μL |
Nuclear-Free water | 4.2μL | 37.8μL |
5μL |
Component | 1× | 16× |
SYBR(2×) | 10μL | 16μL |
cDNA | 1μL | 16μL |
Nuclear-Free water | 4μL | 64μL |
15μL |
Component | 1× | 5× |
Primer F+R(10μM) | 0.8μL | 4μL |
Nuclear-Free water | 4.2μL | 21μL |
5μL |
Claims (7)
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CN201810041514.XA CN108251377B (zh) | 2018-01-16 | 2018-01-16 | 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 |
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CN201810041514.XA CN108251377B (zh) | 2018-01-16 | 2018-01-16 | 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 |
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CN108251377A true CN108251377A (zh) | 2018-07-06 |
CN108251377B CN108251377B (zh) | 2021-08-27 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114134103A (zh) * | 2021-11-30 | 2022-03-04 | 北京中因科技有限公司 | 一种高效获得高活力iPSCs单克隆的方法 |
Citations (9)
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WO2004028339A2 (en) * | 2002-09-27 | 2004-04-08 | Brigham And Women's Hospital, Inc. | Treatment of patients with multiple sclerosis based on gene expression changes in central nervous system tissues |
EP1956910A2 (en) * | 2005-11-23 | 2008-08-20 | The Board of Regents of The University of Texas System | Oncogenic ras-specific cytotoxic compound and methods of use thereof |
EP1960777A2 (en) * | 2005-11-28 | 2008-08-27 | Choongwae Pharma Corporation | Serum-free expansion of cells in culture |
WO2009061442A1 (en) * | 2007-11-06 | 2009-05-14 | Children's Medical Center Corporation | Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells |
CN102325531A (zh) * | 2008-12-19 | 2012-01-18 | 健泰科生物技术公司 | 化合物和使用方法 |
CN102741394A (zh) * | 2009-10-16 | 2012-10-17 | 斯克里普斯研究所 | 多能细胞的诱导 |
CN103114075B (zh) * | 2013-01-18 | 2014-11-12 | 内蒙古大学 | 一种牛滋养层干细胞建系方法 |
CN106754729A (zh) * | 2016-12-31 | 2017-05-31 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用Xist Tale抑制性转录因子制备诱导性多能干细胞的方法 |
CN109750000A (zh) * | 2017-11-06 | 2019-05-14 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用XistTale抑制性转录因子R6制备新型动物细胞系R6-MEFs方法及用途 |
-
2018
- 2018-01-16 CN CN201810041514.XA patent/CN108251377B/zh active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004028339A2 (en) * | 2002-09-27 | 2004-04-08 | Brigham And Women's Hospital, Inc. | Treatment of patients with multiple sclerosis based on gene expression changes in central nervous system tissues |
EP1956910A2 (en) * | 2005-11-23 | 2008-08-20 | The Board of Regents of The University of Texas System | Oncogenic ras-specific cytotoxic compound and methods of use thereof |
CN101360422A (zh) * | 2005-11-23 | 2009-02-04 | 得克萨斯大学体系董事会 | 致癌Ras特异性细胞毒化合物及其使用方法 |
US20090286847A1 (en) * | 2005-11-23 | 2009-11-19 | Board Of Regents, The University Of Texas System | Oncogenic ras-specific cytotoxic compound and methods of use thereof |
EP1960777A2 (en) * | 2005-11-28 | 2008-08-27 | Choongwae Pharma Corporation | Serum-free expansion of cells in culture |
WO2009061442A1 (en) * | 2007-11-06 | 2009-05-14 | Children's Medical Center Corporation | Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells |
CN102325531A (zh) * | 2008-12-19 | 2012-01-18 | 健泰科生物技术公司 | 化合物和使用方法 |
CN102741394A (zh) * | 2009-10-16 | 2012-10-17 | 斯克里普斯研究所 | 多能细胞的诱导 |
CN103114075B (zh) * | 2013-01-18 | 2014-11-12 | 内蒙古大学 | 一种牛滋养层干细胞建系方法 |
CN106754729A (zh) * | 2016-12-31 | 2017-05-31 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用Xist Tale抑制性转录因子制备诱导性多能干细胞的方法 |
CN109750000A (zh) * | 2017-11-06 | 2019-05-14 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用XistTale抑制性转录因子R6制备新型动物细胞系R6-MEFs方法及用途 |
Non-Patent Citations (3)
Title |
---|
JINGWEI WEI等: "KDM4B-mediated reduction of H3K9me3 and H3K36me3 levels improves somatic cell reprogramming into pluripotency", 《SCIENTIFIC REPORTS》 * |
梁延峰: "Xist Tale R-dTFs来源的小鼠iPS细胞系的建立及多能性鉴定", 《中国优秀硕士学位论文全文数据库(电子期刊)基础科学辑》 * |
梁延峰等: "Xist Tale R-dTFs来源的小鼠iPS细胞系的建立及多能性鉴定", 《中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会论文集》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114134103A (zh) * | 2021-11-30 | 2022-03-04 | 北京中因科技有限公司 | 一种高效获得高活力iPSCs单克隆的方法 |
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Inventor after: Zhang Jindun Inventor after: Li Xihe Inventor before: Zhang Jindun Inventor before: Li Xihe Inventor before: Liang Yanfeng Inventor before: Fan Lihong |
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Effective date of registration: 20190611 Address after: 010020 No. 235 West University Road, Saihan District, Hohhot City, Inner Mongolia Autonomous Region Applicant after: Inner Mongolia University Applicant after: INNER MONGOLIA SAIKEXING LIVESTOCK BREEDING AND SEED INDUSTRY BIOTECHNOLOGY RESEARCH INSTITUTE CO., LTD. Applicant after: Inner Mongolia saikexing reproductive biotechnology (Group) Limited by Share Ltd Address before: 011517 Jinle Economic Zone, Helingeer City, Hohhot, the Inner Mongolia Autonomous Region. The intersection of Jinsheng road and East Valley Road of Sheng Le modern service area Applicant before: INNER MONGOLIA SAIKEXING LIVESTOCK BREEDING AND SEED INDUSTRY BIOTECHNOLOGY RESEARCH INSTITUTE CO., LTD. Applicant before: Inner Mongolia saikexing reproductive biotechnology (Group) Limited by Share Ltd |
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