CN108192902A - 一种多肽 - Google Patents

一种多肽 Download PDF

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CN108192902A
CN108192902A CN201711457491.2A CN201711457491A CN108192902A CN 108192902 A CN108192902 A CN 108192902A CN 201711457491 A CN201711457491 A CN 201711457491A CN 108192902 A CN108192902 A CN 108192902A
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马丁·约翰内斯·勒斯纳
弗里茨·艾兴泽埃尔
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Abstract

本发明涉及一种多肽。本发明还涉及所述多肽相应的核酸分子，包含上述核酸分子的构建体和/或载体和/或细胞，和/或包含所述多肽、核酸分子、构建体、载体和/或细胞的组合物。本发明进一步涉及上述组合物用于医疗用途，优选用于治疗传染病。此外，本发明涉及所述多肽、核酸分子、构建体、载体、细胞和/或组合物作为抗菌剂、优选作为食品添加剂或消毒剂的应用，或用于检测细菌，优选用于诊断应用。

Description

一种多肽
本申请是申请日为2011年5月4日的题为“一种多肽”的中国专利申请号201180072127.9的分案申请。
技术领域
本发明涉及一种多肽，相应的核酸分子，包含上述核酸分子的构建体和/或载体和/或细胞，和/或包含所述多肽、核酸分子、构建体、载体和/或细胞的组合物。本发明进一步涉及上述多肽、相应的核酸分子、包含上述核酸分子的构建体和/或载体和/或细胞、和/或组合物，用于医疗用途，优选用于治疗传染病。此外，本发明涉及所述多肽、核酸分子、构建体、载体、细胞和/或组合物作为抗菌剂、优选作为食品添加剂或消毒剂的应用，或在用于检测细菌、优选用于诊断应用中的应用。
背景技术
金黄色葡萄球菌是一种主要的人类病原体，其通常牵涉若干严重的传染病和食物中毒。由于新出现的耐抗生素菌株，它的治疗变得越来越困难。来自感染金黄色葡萄球菌的噬菌体的内溶素已表明潜在地控制这些病原体并可以用于它们的特异性检测。在大多数情况下，靶向葡萄球菌物种的内溶素应用的主要障碍是低酶活性、难以大批量生产和/或蛋白质稳定性。
总是需要关于例如抗微生物活性和/或稳定性具有改善特性的新抗菌剂。
发明内容
本文报道的是新表征的Ply2638，金黄色葡萄球菌噬菌体Φ2638a的内溶素。上述酶和若干工程化衍生物以可溶性方式表达在大肠杆菌中并在冻干(冷冻干燥，lyophilisation)以后显示出乎意料的稳定性，如在重建(重构，reconstitution)以后由它们的裂解活性所证明的。除细胞壁结合域(其结合葡萄球菌属的细胞壁)之外，我们还示出，两个功能域，即M23内肽酶域和酰胺酶域对于最佳的裂解活性是至关重要的。
我们示出，具有催化结构域的酶的改造和/或源自金黄色葡萄球菌Φ11内溶素、ΦTwort内溶素、和溶葡萄球菌素的细胞壁结合域的复制产生异源多肽融合产物，其在冻干和重建以后具有增强的裂解活性和/或改变的pH最适点(最适度，optimum)和/或增加的稳定性。
核酸分子
在第一方面，提供了一种核酸分子，其包括第一核苷酸序列或由第一核苷酸序列组成，所述核苷酸序列与SEQ ID NO:12具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性(关于所有本文使用的SEQ ID NO的概述参见表1)。优选地，所述第一核苷酸序列具有至少282、285、290、300、310、320、330、340、350、360或370个核苷酸，更优选至少381个核苷酸的长度，和/或至多510、480、450、420或390个核苷酸的长度。优选地，所述核酸分子具有至少282、285、290、300、310、320、330、340、350、360、370个核苷酸，更优选至少381个核苷酸的长度，和/或至多4500、4200、3900和/或3600个核苷酸的长度。优选地，所述核酸分子具有至少1200、1230、1260、1290、1320、1350、1380、1410、1440、1470、1500、1530或1560个核苷酸的长度，和/或至多4500、4200、3900和/或3600个核苷酸的长度。还优选的是，按照本发明的核酸分子具有至少1890、1920、1950、1980、2010、2040、2070、2100、2130或2160个核苷酸的长度，和/或至多4500、4200、3900和/或3600个核苷酸的长度。优选地，所述第一核苷酸序列编码细胞壁结合域，其结合葡萄球菌属的肽聚糖细胞壁。优选地，所述第一核苷酸序列源于金黄色葡萄球菌噬菌体Φ2638a内溶素。
如估计自与ALE-1的C端92个残基的晶体结构的序列对比(Lu et al.,J.Biol.Chem.,2006,281(1):549-58)，据估计，来自源自金黄色葡萄球菌噬菌体Φ2638a内溶素的细胞壁结合域的最少94个氨基酸可以足以将上述酶引导到葡萄球菌属的细胞壁。
可以利用技术人员公知的测定来评估(结构)域与葡萄球菌属的肽聚糖细胞壁的结合。在优选的实施方式中，免疫组织化学技术和/或基因融合技术(其产生标记的构建体)用来评估肽、多肽或蛋白质与葡萄球菌属的肽聚糖细胞壁的特异性结合。在上述免疫组织化学或融合技术中所使用的信号的定量方法是本领域公知的。
在一种实施方式中，可以利用荧光融合构建体来量化葡萄球菌属肽聚糖细胞壁结合，上述荧光融合构建体包含多肽，其包含由第一核苷酸序列编码的域。由Loessner et al(Molecular Microbiology 2002,44(2):335–349)以及在实施例1中详细描述了这样的细胞壁结合测定。在该测定中，使包含所述荧光融合构建体或阴性对照、优选绿色荧光蛋白(GFP)的溶液经受葡萄球菌属细胞、优选金黄色葡萄球菌细胞、更优选金黄色葡萄球菌BB255指定时间段，其后通过离心作用，和结合的荧光融合构建体一起，沉降细胞。暴露于荧光融合构建体的葡萄球菌属细胞的荧光信号减去暴露于阴性对照(优选GPF)的葡萄球菌属细胞的荧光信号是细胞结合的度量，如在本公开中所指的。
优选地，在本发明的范围内，核酸分子将编码多肽域，该多肽域结合葡萄球菌属的肽聚糖细胞壁，当使用这种测定时，检测到沉降细胞的荧光信号的增加高于阴性对照(如本文中所定义的)。上述结合优选是特异性的。优选地，本发明涉及核酸分子，该核酸分子编码多肽或域，其呈现由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素(Ply2638)的肽聚糖细胞壁结合如本文所定义的至少50、60、70、80、90或100、150或200％的结合。
在一种实施方式中，本发明涉及核酸分子，其与编码金黄色葡萄球菌噬菌体Ф2638内溶素的SEQ ID NO:1具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。
本发明进一步涉及核酸分子，除所述第一核苷酸序列之外，其还包含编码裂解域的异源核苷酸序列。优选地，所述裂解域呈现肽聚糖水解酶活性(如后文中所定义的)。所述核酸分子包含异源核苷酸序列，其在本文中被定义为“改造构建体(retrofittedconstruct)”。
如在本文中所使用的，术语"异源序列"或"异源核酸"是指这样的核酸，其不是天然存在的，可操作地连接为所述第一核苷酸序列的邻近序列。如在本文中所使用的，术语“异源”可以指“重组体”。"重组体"是指这样的遗传本体(entity)，其不同于一般在天然界中发现的遗传本体。当应用于核苷酸序列或核酸分子时，这意味着所述核苷酸序列或核酸分子是克隆、限制和/或连接步骤、和其它程序(其致使产生不同于在天然界中发现的序列或分子的构建体)的各种组合的产物。
可以通过技术人员公知的方法来评估“肽聚糖水解酶活性”，其在本文中还被定义为“裂解活性”。在实施方式中，可以用分光光度法并通过测量底物细胞悬浮液的浊度降低来评估裂解活性。优选地，可以用分光光度法测量金黄色葡萄球菌悬液的浊度降低来评估裂解活性，其中通过用分光光度法(Libra S22,Biochrom)测量OD595来量化浊度。更优选地，在37℃下，在PBS缓冲液(pH 7.4，120mM氯化钠)中，连同金黄色葡萄球菌悬浮液(其具有1±0.05的初始OD600，如用分光光度法(Libra S22,Biochrom)所评估的)一起，温育200nM的由核酸分子(如在本文中识别的)编码的多肽30分钟。通过将在温育30分钟以前的OD595减去在温育30分钟以后的OD595来计算浊度降低。在本发明的范围内，核酸分子将包含编码裂解域的核酸序列，当利用这种测定时，检测到至少10、20、30、40、50或60％的浊度降低。优选地，检测到至少70％的降低。优选地，本发明涉及核酸分子，该核酸分子编码多肽，其呈现由SEQID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素(Ply2638)的裂解活性的至少50、60、70、80、90、100、150或200％或更高的裂解活性。
在实施方式中，本发明的核酸分子可以不包含SEQ ID NO:1或者不由SEQ ID NO:1构成。SEQ ID NO:1编码金黄色葡萄球菌噬菌体Φ2638内溶素。
优选的实施方式包括核酸分子，该核酸分子包含所述第一核苷酸序列(如本文中所识别的)并进一步包含作为裂解域的第二和第三核苷酸序列，其中所述第二序列编码内肽酶域以及第三核苷酸序列编码酰胺酶域。因此，本发明涉及核酸分子，该核酸分子包含所述第一核苷酸序列，其中所述核酸分子与SEQ ID NO:1具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性，或其中所述核酸分子进一步包含编码裂解域的异源核苷酸序列。
如在本文中所使用的，内肽酶域优选切割五甘氨酸交联桥(cross-bridge)(Trayer,H.R.and Buckley,C.E.(1970)Molecular properties of lysostaphin,abacteriolytic agent specific for Staphylococcus aureus.J.Biol.Chem.245,4842–4846)，其存在于葡萄球菌属的细胞壁中，优选存在于金黄色葡萄球菌、模仿葡萄球菌和肉葡萄球菌的细胞壁中。如在本文中所使用的，酰胺酶域优选水解包含γ-谷氨酰基的底物。在用纯化肽聚糖温育包含任何这些域的所述多肽以后，可以通过表征切割产物来证实在多肽中这些域的功能和活性。优选地，编码第二或第三域的每个核苷酸序列具有细菌或噬菌体来源。在优选的实施方式中，所述第二和第三核苷酸序列源于编码酶的基因，上述酶选自由金黄色葡萄球菌噬菌体Φ2638a内溶素、金黄色葡萄球菌噬菌体Φ11内溶素、金黄色葡萄球菌噬菌体ΦTwort内溶素和模仿葡萄球菌溶葡萄球菌素组成的组。优选地，所述第二核苷酸序列与SEQ ID NO:14或15具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性以及所述第三核苷酸序列与SEQ ID NO:17或18具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％序列同一性。
本发明包括如本文所定义的所有构建体，其包含在构建体内的任何可能位置处的功能域(如在本发明中所指的)。在优选的实施方式中，如本文所定义的核酸分子编码多肽，该多肽具有由如本文中所识别的第一核酸序列编码的C端域，其在本文中显示编码能够靶向葡萄球菌属的功能多肽。甚至更优选的是如本文所定义的核酸分子，包括这样的核酸分子，其与SEQ ID NO:9具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。SEQ ID NO:9包含第一核苷酸序列，所述第一核苷酸序列编码C端SH3b同源细胞壁结合域(两个域均来自由SEQ ID NO:1编码的Ply2638：Leu138-Lys486)；第二核苷酸序列，该第二核苷酸序列编码多肽，其包含N端M23甘氨酰-甘氨酸内肽酶同源域(由SEQ ID NO:33编码的成熟溶葡萄球菌素：Ala1-Gly154)；以及第三核苷酸序列，其编码中心酰胺酶-2同源域。它具有58.266kDa的理论大小。由SEQ ID NO:9编码的多肽与金黄色葡萄球菌噬菌体Φ2638a内溶素的不同之处在于：N-端M23内肽酶域被来自模仿葡萄球菌溶葡萄球菌素的M23内肽酶域取代。我们在这里示出，由SEQ ID NO:9编码的多肽，相比于金黄色葡萄球菌噬菌体Φ2638a内溶素，显示至少20％增加的裂解活性，同时在冻干和重建以后保持裂解活性。在优选的实施方式中，包含所述第一、第二和第三核苷酸序列的核酸分子编码多肽，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，其呈现至少0.7、0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5、1.6、1.7、1.8、1.9或2倍的裂解活性。在优选的实施方式中，包含所述第一、第二和第三核苷酸序列的核酸分子编码多肽，相比于新制备的多肽，其在冻干和重建(如本文所定义的)以后，呈现至多1、2、3、4、5、6、7、8、9或10％的裂解活性的降低，其中“新制备的”在本文中优选被定义为，在-20℃下，以1.63mg/mL在冻干缓冲液(50mM Tris、500mM蔗糖、200mM甘露醇、0.05％聚山梨酯20+50％甘油)中储存至多2天，并且在测定中评估裂解活性(如本文中所确定的)之前立即融化。
冻干和重建在本文中被定义为通过冷冻干燥脱水并随后通过添加水重建样品。在实施方式中，可以通过针对3次变化的300ml冻干缓冲液(50mM磷酸盐或Tris、500mM蔗糖、200mM甘露醇、pH 7.4)等分部分进行透析(渗析)并在液氮的气相中进行冷冻来进行冻干和重建。可以在标准条件下进行冷冻干燥，优选地在-40℃和75毫托的真空下60分钟，接着在5小时内提高温度至-10℃以及在-10℃和相同真空水平下保持另外60分钟。作为最后的步骤，优选在10小时内将温度提高至25℃。通过添加水重建样品。
在另一种优选的实施方式中，如本文所定义的核酸分子除上文识别的第一、第二和第三核苷酸序列之外还包含至少一个相同或异源的第一、第二和/或第三核苷酸序列的复制体。优选地，如本文所定义的核酸分子除所述第一、第二和第三核苷酸序列之外还包含重复的相同第一核苷酸序列。我们在这里示出，如本文所定义的编码细胞壁结合域的第一核苷酸序列的复制产生多肽，优选地如由SEQ ID NO:20编码的，相比于由缺少上述重复的第一域的核酸分子编码的参比多肽的裂解活性，或相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，其显示至少5、10、20、30、20或40％增加的裂解活性，如在如本文中所确定的测定中所评估的，更具体地利用等摩尔量的多肽和改变的PBS缓冲液，其包含200、300、400、或1000nM NaCl。该实施方式还包括异源核酸分子，其中所述第一、第二和第三核苷酸序列源于相同来源，其为金黄色葡萄球菌噬菌体Φ2638a，所述核酸分子包含以下序列，其与SEQ ID NO:1具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性，以及另外的重复的、相同的或异源的第一、第二和/或第三核苷酸序列。还优选的是，如本文所定义的核酸分子包含核苷酸序列，其与SEQ ID NO:6和20具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。
在另一种优选的实施方式中，如本文所定义的核酸分子包含编码CHAP(半胱氨酸、组氨酸依赖性酰胺水解酶/肽酶)域的第四核苷酸序列。更优选地，所述第四核苷酸序列源于金黄色葡萄球菌噬菌体Φ11或金黄色葡萄球菌噬菌体ΦTwort内溶素。甚至更优选地，所述第四核苷酸序列与SEQ ID NO:18或SEQ ID NO:19具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。优选地，如本文所定义的核酸分子包含核苷酸序列，其与SEQ ID NO:5或7具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。我们在这里示出以下分子，其包含如由SEQ ID NO:5所定义的所述第一、第二、第三和第四核苷酸序列，编码以下多肽，相比于由缺少所述第四域的构建体编码的多肽，和/或相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素，其呈现增加的裂解活性和/或改变的、优选降低的pH最适点。用分光光度法并在如前文所定义的条件下评估裂解活性。优选地，相比于由参比多肽编码的多肽(与所述多肽的差别仅在于缺少所述第四域)的裂解活性，或相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，所述多肽呈现至少0.7、0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5、1.6、1.7、1.8、1.9、或2倍的裂解活性的增加。甚至更优选地，相比于限定的参比多肽或由SEQ ID NO:1编码的多肽，所述多肽呈现至少2.5倍的裂解活性的增加。
改变的或降低的pH最适点在本文中被定义为最佳的裂解活性改变或降低至较低的pH值，其中离子强度保持恒定。优选地用分光光度法评估裂解活性(如本文所定义的)。优选地，相比于由参比多肽编码的多肽(与所述多肽的差别仅在于缺少所述第四域)的裂解活性，或相比于由SEQ IDNO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，裂解活性的pH最适点降低0.5-1pH单位。
本发明优选涉及包含第一、第二、第三和可选地第四核苷酸序列(如本文中所识别的)的核酸分子，其编码以下多肽，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，其具有相同的裂解活性和/或相同的pH最适点或者其具有增加的裂解活性和/或降低的pH最适点。当利用如本文中所确定的测定或技术人员公知的方法时，在本文中识别的核酸分子没有可检测的差异。本发明还涉及包含如本文中所识别的第一、第二、和第四核苷酸序列的核酸分子，其编码以下多肽，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，其具有相同的裂解活性和/或相同的pH最适点，或者其具有增加的裂解活性和/或降低的pH最适点。本发明还涉及包含如本文中所识别的第一、第三、和第四核苷酸序列的核酸分子，其编码以下多肽，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的裂解活性，其具有相同的裂解活性和/或相同的pH最适点，或者其具有增加的裂解活性和/或降低的pH最适点。本文识别的每种核苷酸序列，即第一、第二、第三、第四核苷酸序列，其编码本文定义的多肽的单独域，可以通过已知用于构建和装配核酸片段的任何常用方法加以装配，其中上述常用方法是本领域技术人员公知的并且广泛描述于文献(Sambrook,Maniatis et al.(1989))和本公开描述的实验部分。在优选的实施方式中，第一、第二、第三和/或第四核苷酸序列可操作地连接在一起。
因此，本发明的核酸分子编码多肽，优选如本文中所识别的多肽，其能够借助于由如本文所定义的第一核苷酸序列编码的细胞壁结合域来结合葡萄球菌属，和/或溶解所述细菌，其中借助于分别由第二、第三和第四核苷酸序列编码的内肽酶和/或酰胺酶域和可选地CHAP域(如本文所定义的)。
在优选的实施方式中，如本文所定义的本发明的核酸分子可选地包含以下序列，其编码便于纯化的标记。优选地，所述标记选自，但不限于，由FLAG标记、多(His)标记、HA标记和Myc标记组成的组。更优选地，所述标记是6xHis标记。甚至更优选地，所述标记是N端6xHis标记，其相同于SEQ ID NO:43。
多肽
在另外的方面，提供了由如上文识别的核酸分子编码的多肽。这种多肽包含细胞壁结合域，并且优选地包含内肽酶域和/或酰胺酶域(如在之前的部分中所定义的)。
本发明包括的多肽域与SEQ ID NO:35、36、37、38、39、40、41和/或42优选具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。优选地，本发明包括的多肽域优选包含一个或多个假定接头(连接子，linker)并与SEQIDNO:51、52、53、54、55、56、57和/或58具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。
本发明包括的多肽与SEQ ID NO:21、25、26、27、29和/或32优选具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。更优选地，本发明包括的多肽与SEQ ID NO:25、26、27、29和/或32优选具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性。
在本发明的实施方式中，多肽与SEQ ID NO:21、25、26、27、29、32、35、36、37、38、39、40、41、和/或42优选具有至少80％的序列同一性，其由核酸构建体编码，上述核酸构建体与SEQ ID NO:1、5、6、7、9、20、12、13、14、15、16、17、18和/或19分别具有至少80％的同一性。更优选地，本发明的多肽与SEQ ID NO:25、26、27、29、32、35、36、37、38、39、40、41、和/或42具有至少80％的序列同一性，其由核酸构建体编码，上述核酸构建体与SEQ ID NO:5、6、7、9、20、12、13、14、15、16、17、18和/或19分别具有至少80％的同一性。
按照本发明的多肽可以具有至少94、95、96、100、110或120个氨基酸，优选127个氨基酸和/或至多1500、1400、1300或1200个氨基酸的长度。优选地，所述多肽的长度为至少400、410、420、430、440、450、460、470、480、490、500、510或520个氨基酸和/或至多1500、1400、1300或1200个氨基酸。还优选的是，根据本发明的多肽的长度为至少630、640、650、660、670、680、690、700、710或720个氨基酸和/或至多1500、1400、1300或1200个氨基酸。
本发明包括的氨基酸或核苷酸序列可以源自如本文中所识别的序列中的一种，其中分别取代、插入、缺失、或添加1、2、3、4、5、6、7、8、9、10、12、14、16、18、20或更多个核苷酸或氨基酸。本发明包括的氨基酸序列可以源自如本文中所识别的序列中的一种，其中通过添加另外的N端或C端氨基酸或化学部分，以增加稳定性、溶解性和活性。
本发明的实施方式包括变体多肽。变体多肽可以是多肽的非天然存在形式。多肽变体可以以一些工程化方式不同于分离自其天然来源的多肽。可以通过开始自SEQ ID NO:1、5、6、7、9、12、13、14、15、16、17、18、19和/或20的核苷酸序列的定位诱变来制备变体。优选地，多肽变体包含这样的突变，其并不改变编码的多肽的生物功能。按照优选的实施方式，多肽变体呈现葡萄球菌属肽聚糖细胞壁结合和/或裂解活性，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的葡萄球菌属肽聚糖细胞壁结合和/或裂解活性，其是相同的或增强的。本发明的多肽变体优选是SEQ ID NO:21、25、26、27、29、32、35、36、37、38、39、40、41和/或42的变体。具有相同的或增强的葡萄球菌属肽聚糖细胞壁结合和/或裂解活性的多肽变体是呈现金黄色葡萄球菌肽聚糖细胞壁结合和/或裂解活性的多肽，相比于由SEQ ID NO:1编码的金黄色葡萄球菌噬菌体Φ2638a内溶素的葡萄球菌属肽聚糖细胞壁结合和/或裂解活性(以如上文确定的测定所测得)，其是相同的或增加的。
按照另一种优选实施方式，本发明的核苷酸序列是SEQ ID NO:1、5、6、7、9、12、13、14、15、16、17、18、19和/或20的核苷酸序列的变体。核苷酸序列变体可以用于制备如之前定义的多肽变体。核酸变体可以是如上文所定义的任何核苷酸序列的片段。核酸变体还可以是核苷酸序列，其与SEQ ID NO:1、5、6、7、9、12、13、14、15、16、17、18、19和/或20的差别在于遗传密码的简并度。核酸变体还可以是SEQ ID NO:1、5、6、7、9、12、13、14、15、16、17、18、19和/或20的等位变体。等位变体表示占据相同染色体基因座的基因的两种或更多种替代形式的任何一种。优选的核酸变体是核苷酸序列，其包含沉默突变。可替换地或组合地，还可以通过引入核苷酸取代来获得核酸变体，上述核苷酸取代并不产生由核苷酸序列编码的多肽的另一种氨基酸序列，而是对应于用于产生本发明的多肽的宿主生物体的密码子选择。按照优选的实施方式，核酸变体编码仍然呈现其生物功能的多肽。更优选地，核苷酸序列变体编码呈现葡萄球菌属肽聚糖细胞壁结合和/或裂解活性的多肽。甚至更优选地，核酸变体编码具有如之前定义的增强的葡萄球菌属肽聚糖细胞壁结合和/或裂解活性的多肽。编码呈现葡萄球菌属肽聚糖细胞壁结合和/或裂解活性的多肽的核酸可以分离自任何微生物。
可以利用技术人员已知的技术来获得所有这些变体，如在低至中等至高杂交条件下通过杂交筛查文库(蛋白质印迹(southern blotting)法)可以用来设计探针的核苷酸序列SEQ ID NO:1、5、6、7、9、12、13、14、15、16、17、18、19和/或20或其变体。低到中到高严格性条件是指在42℃下在5X SSPE、0.3％SDS、200pg/ml剪切和变性鲑鱼精DNA中，以及在分别用于低到中到高严格性的25％、35％或50％甲酰胺中的预杂交和杂交。随后，洗涤杂交反应三次，时间为30分钟，每次利用2X SSC、0.2％SDS以及用于低到中到高严格性的55℃、65℃、或75℃。
如本文提供的序列信息不应狭义地解释为需要包括错误识别的碱基。技术人员能够确定上述错误识别的碱基并且知道如何校正这样的错误。
核酸构建体
在另外的方面，提供了一种核酸构建体，其包含如在前述部分中所识别的核酸分子。这种核酸构建体可以包含第一核酸序列，该第一核酸序列编码以下多肽，其包含细胞壁结合域，可以进一步包含如在前述部分中所定义的第二和第三以及可选的第四核酸序列。
本发明还涉及一种表达载体，其包含本发明的核酸构建体。优选地，表达载体包含本发明的核苷酸序列，其可操作连接于一个或多个控制序列，该控制序列引导编码的多肽在细胞、受试者、或无细胞表达系统中的生产或表达。
表达载体可以被看作是重组表达载体。可以通过引入根据本发明的核酸分子将质粒、黏粒、噬菌体或病毒转化，来构建这种载体。根据待转化的宿主生物体的上述转化载体是本领域技术人员公知的并且广泛描述于文献中。
本发明的又一个主题是用于宿主生物体的转化方法，其中通过整合(合并，integrating)本发明的至少一种核酸分子，可以通过任何适当的已知方式来进行上述转化，其中上述已知方式广泛描述于专题文献以及尤其描述于在本申请中引用的参考文献中，更特别地通过根据本发明的载体。
细胞
在另外的方面，本发明涉及一种细胞，其包含如本文所定义的本发明的核酸构建体或表达载体。细胞可以是任何微生物、原核或真核细胞，其适用于表达本发明的多肽。在优选的实施方式中，所述细胞是大肠杆菌。在甚至更优选的实施方式中，所述细胞是大肠杆菌CL1blue MRF。
方法
在另外的方面，提供了一种用于生产、可选纯化和可选冷冻干燥如在之前部分中所定义的多肽的方法。所述方法包括以下步骤：
i)在细胞中生产所述多肽，其中上述细胞包含如在前述部分中所定义的核酸构建体，可选地，
ii)纯化所述多肽，以及可选地，
iii)冷冻干燥所述纯化多肽。
在优选的实施方式中，大肠杆菌用于步骤i)：用于生产多肽，其中利用重组技术。更优选地，大肠杆菌XL1blueMRF用于步骤i)：用于生产多肽，其中利用重组技术。优选地，在步骤ii)中，填充有5mL低密度镍螯合琼脂糖珠(ABT珠)的IMAC和Econo-Pac层析柱(Biorad)连同重力流一起用于纯化所述(6xHis标记的重组)多肽。可以相对于3x1l冷冻干燥缓冲液透析洗脱的多肽2、4、和12小时，所述缓冲液优选包含50mM磷酸盐、500mM蔗糖、200mM甘露醇、0.005％聚山梨酯20，pH 7.4。
方法
在另外的方面，本发明还涉及用于生产具有增强的裂解活性的多肽的方法，其中通过处理如在前述部分中所定义的或如通过上文描述的方法可获得的多肽。所述处理包括取代二价金属离子，用于增加裂解活性(相比于未经处理的多肽)，优选地所述方法包括以下步骤：
i)相对于包含螯合化合物的缓冲液透析(渗析)所述多肽；
ii)相对于包含二价金属离子的缓冲液透析(渗析)所述多肽，优选地所述二价金属离子选自由Mn2+、Co2+、Cu2+、和Zn2+组成的组。
“螯合化合物”在本文中被定义为结合金属离子的化合物。公知的螯合化合物是乙二胺四乙酸(EDTA)和乙二醇四乙酸(EGTA)。优选地，EDTA用于本发明的方法的步骤i)。
优选地，步骤ii)的二价金属离子选自由Mn2+、Co2+、Cu2+组成的组，更优选地，所述二价金属离子选自由Mn2+和Co2+组成的组，甚至更优选地，所述二价金属离子是Mn2+。
我们示出，通过上文限定的任一种取代二价金属离子致使Ply2638的裂解活性增加2-2.5倍。用如本文所定义的分光光度法来评估裂解活性。优选地，相比于未经处理的多肽，所述方法致使裂解活性增加至少1.1、1.2、1.3、1.4、1.5、1.6、1.7、1.8、1.9或2倍。甚至更优选地，上述方法致使裂解活性增加至少2.5倍。优选地，相比于未经处理的由SEQ IDNO:1编码的多肽，经处理的多肽呈现裂解活性增加0.7、0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5、1.6、1.7、1.8、1.9至2倍。
组合物
在另外的方面，提供了一种组合物，该组合物包含如本文中所识别的或通过本文描述的方法可获得的核酸分子或核酸构建体或多肽或载体或细胞。优选地，本发明涉及一种组合物，该组合物呈现如本文所定义的裂解活性。更优选地，所述组合物用作药剂。这种药剂优选用于治疗、预防和/或延迟传染病。本发明还涉及一种药物或药用组合物。甚至更优选地，本发明涉及一种用于治疗传染病的药物或药用组合物。优选地，本发明涉及一种药物组合物，用于治疗由细菌、优选地葡萄球菌属的细菌、更优选地金黄色葡萄球菌种的细菌引起的传染病。优选地，所述传染病是皮肤感染、乳腺炎、肺炎、脑膜炎、心内膜炎、中毒性休克综合征(TSS)、脓毒症、败血症、菌血症、或骨髓炎。优选地，所述皮肤感染选自由丘疹、脓疱病、疖、疔疮、蜂窝织炎、毛囊炎、痈、鳞状皮肤综合征和脓肿组成的组。
如本文所定义的组合物可以包含如本文中所识别的或通过本文描述的方法可获得的不同核酸分子、和/或核酸构建体和/或多肽和/或载体和/或细胞的混合物。
如本文所定义的组合物可以包含一种或多种另外的活性组分。活性组分优选地在本文中被定义为显示如本文所定义的裂解活性。优选地，所述一种或多种另外的活性组分选自由噬菌体或抗菌素和抗生素组成的组。在本文中涵盖的噬菌体可以是在文献中已知的任何噬菌体。优选地，本发明所包括的噬菌体属于，但不限于，由肌噬菌体科(Myoviridae)、长尾噬菌体科(Siphoviridae)和短尾噬菌体科(Podoviridae)组成的列表中的科。本发明所包括的噬菌体还可以属于由复层噬菌体科(Tectiviridae)、覆盖噬菌体科(被脂病毒科，Corticoviridae)、脂毛噬菌体科(脂毛病毒科，Lipothrixviridae)、芽生噬菌体科(Plasmaviridae)、古噬菌科(Rudiviridae)、微小纺锤形噬菌体科(福赛尔噬菌体科，Fuselloviridae)、丝杆噬菌体科(Inoviridae)、微小噬菌体科(Microviridae)、轻小噬菌体科(Leviviridae)和囊状噬菌体科(Cystoviridae)组成的列表中的科。更优选地，所述一种或多种活性组分包含溶葡萄球菌素和/或由溶葡萄球菌素构成，优选与SEQ ID NO:34具有80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99或100％的序列同一性的模仿葡萄球菌溶葡萄球菌素，更优选SEQ ID NO:34的模仿葡萄球菌溶葡萄球菌素。甚至更优选地，所述一种或多种活性组分包含一种或多种不同噬菌体以及溶葡萄球菌素和/或由上述两者组成，优选地，一种或多种不同噬菌体以及模仿葡萄球菌溶葡萄球菌素(SEQ ID NO:34)。在本发明的范围内，可以顺序和/或同时给予如本文所定义的活性组分的组合。
如本文所定义的组合物可以进一步包含药用载体。上述组合物优选用作药物或药剂。优选地，药剂用于治疗传染病。组合物可以具有液体、固体、或者半液体或半固体形式。
本发明的组合物可以用来治疗感染金黄色葡萄球菌的动物(包括人类)。任何适宜的给予途径可以用来给予所述组合物，包括，但不限于：口服、气雾剂或用于递送到肺的其它装置、鼻喷入、静脉内、肌内、腹腔内、鞘内、阴道、直肠、局部、腰椎穿刺、鞘内、以及直接施用到脑和/或脑脊膜。
包含如本文中所识别的或通过本文描述的方法可获得的核酸分子或核酸构建体或多肽或载体或细胞的组合物，当它降低在患者中或在所述患者的细胞中或在细胞系中或在无细胞体外系统中存在的葡萄球菌属的量时，优选是活性的、功能性的、或治疗活性的，或者能够治疗、预防和/或延缓传染病，以及优选意味着葡萄球菌属的初始量的99％、90％、80％、70％、60％、50％、40％、30％、20％、10％、5％或更少仍然是可检测的。优选地，没有葡萄球菌属是可检测的。在此段落中，短语“葡萄球菌属的量”优选指活的葡萄球菌属。可以利用技术人员已知的标准技术来检测葡萄球菌属，这些标准技术如免疫组织化学技术(利用葡萄球菌属特异性抗体)、管凝固酶测试(其检测葡萄球菌凝固酶或"游离凝固酶")、表面蛋白如凝聚因子(玻片凝固酶测试)和/或蛋白A(商用胶乳测试)的检测。可以利用技术人员已知的标准技术来检测活的葡萄球菌属，如微生物细菌培养技术和/或实时定量反转录聚合酶链反应(用来测定细菌mRNA)。优选地，在组织中或者在个体或患者的细胞中，通过比较在用本发明的所述组合物或多肽进行治疗以前在所述个体或患者中存在的量，评估所述降低。可替换地，可以与所述个体或患者的组织或细胞进行比较，其中在治疗是局部的情况下上述组织或细胞还未用所述组合物或多肽加以治疗。
可以将包含如本文中所识别的或通过本文描述的方法可获得的核酸分子或核酸构建体或多肽或载体或细胞的组合物给予患者或所述患者的细胞、组织或器官至少一周、一个月、六个月、一年或更长时间。
在另一种实施方式中，本发明涉及一种非医疗用组合物，其呈现如本文所定义的结合和/或裂解活性。优选地，本发明涉及一种抗菌剂。优选地，本发明涉及一种抗菌剂，其用于溶解细菌、优选葡萄球菌属的细菌、更优选金黄色葡萄球菌种的细菌。优选地，本发明涉及一种抗菌剂，其作为食品防腐剂或消毒剂。
应用
在另外的方面，本发明涉及包含由如本文所定义的第一、第二、第三和可选的第四核酸序列编码的域的多肽的应用，编码上述多肽的核酸分子的应用，包含上述核酸分子的构建体的应用，包含上述构建体的载体的应用，包含上述载体的细胞的应用，和/或包含任何上述的组合物的应用，优选作为抗菌剂。优选地，本发明涉及作为抗菌剂的应用，用于溶解细菌、优选葡萄球菌属的细菌、更优选金黄色葡萄球菌种的细菌。优选地，本发明涉及作为食品防腐剂或消毒剂的抗菌剂。可能地，上述食品防腐剂或消毒剂连同其它抗菌剂一起使用。优选地，上述食品防腐剂或消毒剂连同如本文所定义的一种或多种另外的活性组分一起使用。优选地，所述一种多种另外的活性组分选自由如本文所定义的噬菌体或抗菌素和抗生素组成的组。
上面提到的多肽、核酸分子、构建体、载体、细胞和/或组合物可以通过许多方式应用于食品、和/或各种待消毒的物理部位，上述方式包括，但不限于，将所述多肽和/或包含本发明的多肽的细胞掺入食品，将所述多肽和/或包含本发明的多肽的细胞喷洒到食品或待消毒的物理部位上。
本发明的多肽可以分离自细胞，或可以直接应用或给予包含本发明的所述多肽的细胞而无需所述多肽的分离。例如，可以将产生本发明的多肽的细胞给予受试者(人或动物)或应用于表面，其中本发明的多肽将被分泌进入食品，分泌到表面或分泌进入受试者的肠中。然后，本发明的多肽可以结合并可选地溶解在此环境中存在的细菌细胞，优选葡萄球菌属的细菌，更优选金黄色葡萄球菌种的细菌。
进一步提供了包含由如本文所定义的第一核酸序列编码的域的多肽的应用、编码上述多肽的核酸分子的应用、包含上述核酸分子的构建体的应用、包含上述构建体的载体的应用、包含上述载体的细胞的应用、和/或包含任何上述的组合物的应用，优选用于检测细菌，更优选用于检测葡萄球菌属的细菌，更优选金黄色葡萄球菌种的细菌。优选地，所述多肽、核酸分子、构建体、载体、细胞和/或组合物用于诊断应用。可能地，所述多肽、核酸分子、构建体、载体、细胞和/或组合物连同其它检测剂一起使用。
方法
在另外的方面，本发明进一步涉及通过给予如前文所定义的组合物用于治疗、延缓和/或预防传染病的方法。这种方法的所有特点已在本文中加以定义。
定义
序列同一性
"序列同一性"在本文中被定义为在两个或更多氨基酸(肽、多肽、或蛋白质)序列或两个或更多核酸(核苷酸、多核苷酸)序列之间的关系，如通过比较序列所确定的。在本领域中，"同一性"还指在氨基酸或核苷酸序列(根据具体情况)之间序列相关的程度，如通过在一系列上述序列之间的匹配所确定的。通过比较一种肽或多肽的氨基酸序列和它的保守氨基酸取代与第二肽或多肽的序列来确定在两个氨基酸序列之间的"相似性"。在优选的实施方式中，针对如本文中所识别的整个SEQ ID NO计算同一性或相似性。可以通过已知方法容易地计算"同一性"和"相似性"，包括但不限于以下方法，其描述于ComputationalMolecular Biology,Lesk,A.M.,ed.,Oxford University Press,New York,1988；Biocomputing:Informatics and Genome Projects,Smith,D.W.,ed.,Academic Press,New York,1993；Computer Analysis of Sequence Data,Part I,Griffin,A.M.,andGriffin,H.G.,eds.,Humana Press,New Jersey,1994；Sequence Analysis in MolecularBiology,von Heine,G.,Academic Press,1987；和Sequence Analysis Primer,Gribskov,M.and Devereux,J.,eds.,M Stockton Press,New York,1991；以及Carillo,H.,andLipman,D.,SIAM J.Applied Math.,48:1073(1988)。
设计用来确定同一性的优选方法以在待测试的序列之间产生最大匹配。用来确定同一性和相似性的方法编码在可公开获得的计算机程序中。用来在两个序列之间确定同一性和相似性的优选计算机程序方法包括例如，GCG程序包(Devereux,J.,et al.,NucleicAcids Research 12(1):387(1984))、BestFit、BLASTP、BLASTN、和FASTA(Altschul,S.F.etal.,J.Mol.Biol.215:403-410(1990))。BLAST X程序可公开获自NCBI和其它来源(BLASTManual,Altschul,S.,et al.,NCBI NLM NIH Bethesda,MD 20894；Altschul,S.,et al.,J.Mol.Biol.215:403-410(1990))。公知的Smith Waterman算法还可以用来确定同一性。
用于多肽序列比较的优选参数包括以下各项：算法：Needleman和Wunsch,J.Mol.Biol.48:443-453(1970)；比较矩阵：BLOSSUM62，来自Hentikoff和Hentikoff,Proc.Natl.Acad.Sci.USA.89:10915-10919(1992)；空隙罚分(空位罚分)：12；以及空隙长度罚分：4。可用于这些参数的程序可公开获得为来自Genetics Computer Group(位于Madison,WI)的"Ogap"程序。上述参数是用于氨基酸比较的默认参数(连同没有对端间隙的罚分)。
用于核酸比较的优选参数包括以下各项：算法：Needleman和Wunsch,J.Mol.Biol.48:443-453(1970)；比较矩阵：匹配＝+10，错配＝0；空隙罚分：50；空隙长度罚分：3。可获得为Gap程序，来自Genetics Computer Group(位于Madison,Wis)。上面给出的是用于核酸比较的默认参数。
可选地，在确定氨基酸相似性的程度时，技术人员还可以考虑所谓的"保守"氨基酸取代，如技术人员将清楚的。保守氨基酸取代是指具有类似侧链的残基的可互换性。例如，具有脂族侧链的氨基酸的组是甘氨酸、丙氨酸、缬氨酸、亮氨酸、和异亮氨酸；具有脂族羟基侧链的氨基酸的组是丝氨酸和苏氨酸；具有包含酰胺的侧链的氨基酸的组是天冬酰胺和谷氨酰胺；具有芳族侧链的氨基酸的组是苯丙氨酸、酪氨酸、和色氨酸；具有碱性侧链的氨基酸的组是赖氨酸、精氨酸、和组氨酸；以及具有含硫侧链的氨基酸的组是半胱氨酸和甲硫氨酸。优选的保守氨基酸取代组是缬氨酸-亮氨酸-异亮氨酸、苯丙氨酸-酪氨酸、赖氨酸-精氨酸、丙氨酸-缬氨酸、和天冬酰胺-谷氨酰胺。本文披露的氨基酸序列的取代变体是那些变体，其中在所披露的序列中的至少一个残基已被除去并在其位置插入不同的残基。优选地，氨基酸变化是保守的。对于每种天然存在的氨基酸的优选的保守取代是如下：Ala对ser；Arg对lys；Asn对gln或his；Asp对glu；Cys对ser或ala；Gln对asn；Glu对asp；Gly对pro；His对asn或gln；Ile对leu或val；Leu对ile或val；Lys对arg、gln或glu；Met对leu或ile；Phe对met、leu或tyr；Ser对thr；Thr对ser；Trp对tyr；Tyr对trp或phe；以及Val对ile或leu。
核酸构建体、转化、表达载体、可操作地连接、表达、控制序列、多肽
构建体
由核苷酸序列表示核酸分子。由氨基酸序列表示多肽。核酸构建体定义为核酸分子，其分离自天然产生的基因或其已被改变以包含以本来不会存在于天然界的方式被合并或并列的核酸区段。通过核苷酸序列表示核酸分子。可选地，存在于核酸构建体中的核苷酸序列可操作地连接于一个或多个控制序列，其引导所述肽或多肽在细胞或在受试者中产生或表达。
“可操作地连接”在本文中定义为以下构型，其中控制序列被适当地放置在相对于编码本发明的多肽的核苷酸序列的位置，以使控制序列引导本发明的肽或多肽在细胞和/或受试者中的产生/表达。
“可操作地连接”还可以用于定义以下构型，其中序列被适当地放置在相对于编码功能域的另一序列的位置，以使嵌合多肽在细胞和/或受试者中被编码。
“表达”将理解为包括任何涉及肽或多肽的生产的步骤，包括，但不限于，转录、转录后修饰、翻译、翻译后修饰和分泌。
控制序列在本文中定义为包括所有以下组件，其是多肽表达所必要的或者有利于多肽的表达。在最低限度，控制序列包括启动子以及转录和翻译终止信号。可选地，由在核酸构建体中存在的核苷酸序列表示的启动子可操作连接于编码如本文中所识别的肽或多肽的另一种核苷酸序列。
术语"转化"是指在并入新DNA(即，细胞的外源DNA)以后，在细胞中诱导的永久或瞬时基因变化。当细胞是细菌细胞时，如在本发明中所旨在的，该术语通常是指染色体外的、自我复制的载体，其具有可选择的抗生素耐受性。
表达载体可以是任何载体，其可以方便地进行重组DNA程序并且可以引起编码本发明的多肽的核苷酸序列在细胞和/或受试者中的表达。如在本文中所使用的，术语"启动子"是指核酸片段，其作用是控制相对于基因的转录起始位点的转录方向位于上游的一个或多个基因或核酸的转录。它关系到通过针对DNA依赖性RNA聚合酶的结合位点的存在所识别的结合位点、转录起始位点、和任何其它DNA序列，包括，但不限于，转录因子结合位点、阻抑和激活蛋白结合位点、和本领域技术人员已知的直接或间接地用来调节从启动子开始的转录量的任何其它序列的核苷酸。在本发明的上下文中，启动子优选终止于转录起始位点(TSS)的核苷酸-1。
如在本文中所使用的，“多肽”是指任何肽、寡肽。多肽、基因产物、表达产物、或蛋白质。多肽由连续氨基酸组成。术语"多肽"涵盖天然存在的或合成的分子。
在本文件和其权利要求中，动词"包含"和它的结合形式在其非限制性意义上用来指包括在该词语以后的事项，但并不排除未具体提及的事项。另外，动词“由……组成”可以由“基本上由……组成”代替，意思是产物或组合物或如本文所定义的核酸分子或核酸构建体或载体或细胞的肽或多肽可以包含除具体识别的成分以外的另外成分，所述另外成分并不改变本发明的独特特征。另外，通过不定冠词"一"或"一种"提及要素并不排除存在多于一种要素的可能性，除非上下文明显地要求，否则存在一种且仅一种要素。因此不定冠词"一"或"一种"通常是指"至少一种"。在本说明书中引用的所有专利和参考文献的全部内容以引用方式结合于本文。
以下实施例仅用于说明的目的，而不是用来限制本发明的范围。
附图说明
图1对抗金黄色葡萄球菌SA2638/2854细胞，Ply2638(SEQ ID NO:21，由SEQ IDNO:44编码)活性依赖于内溶素浓度的线性关系。在标准条件(PBS缓冲液，pH 7.4，120mM氯化钠)下并用光度裂解测定进行测定。最大活性由来自S形裂解曲线的回归拟合的第一导数确定，借助于SigmaPlot软件计算。误差棒表示标准偏差，其由重复三次的技术实验计算。
图2二价阳离子对Ply2638(SEQ ID NO:21，由SEQ ID NO:44编码)的裂解活性的影响。用EDTA处理酶，随后通过针对包含MgCl2、CaCl2、ZnCl2、CuCl2、CoCl2、或MnSO4的MOPS缓冲液的透析取代金属离子。针对MOPS缓冲液的Ply2638透析(渗析)(省略EDTA处理)用作参比。误差棒表示标准偏差，其由重复三次的技术实验计算。
图3在冻干和重建以后，50nM Ply2638(SEQ ID NO:21，由SEQ ID NO:44编码)对金黄色葡萄球菌SA2638/2854细胞的裂解活性。在标准条件下，用浊度降低测定来测量活性。冻干缓冲液作为对照。在冷冻干燥以后，三域(三重结构域，triple domain)酶恢复全裂解活性。
图4在冻干和重建(表示为冻干)以后，相比于新制备的M23-LST_Ami2638_CBD2638(SEQ ID NO:29，由SEQ ID NO:48编码)和Ply2638(SEQ ID NO:21，由SEQ ID NO:44编码)，50nM M23-LST_Ami2638_CBD2638(SEQ ID NO:29，由SEQ ID NO:48编码)对金黄色葡萄球菌SA2638/2854细胞的裂解活性。冻干缓冲液作为对照。在标准条件下用浊度降低测定来测量活性。在冷冻干燥以后，三域酶恢复全裂解活性。
图5溶葡萄球菌素(LST；SEQ ID NO:34，由SEQ ID NO:33编码)和Ply2638衍生物(SEQ ID NO:31、30、29、21和24，分别由SEQ ID NO:11、10、48、44和4编码)依赖于浓度的相对活性。在50nM下LST的活性设定为参比。在标准条件(37℃，pH 7.4和120mM氯化钠浓度)下并利用来自冷冻储备液的金黄色葡萄球菌SA2638/2854底物细胞进行所有测定。
图6a和图6b在各种pH值(图6a)和氯化钠浓度(图6b)下用浊度降低测定确定的截短和(具有PlyTw域)改造Ply2638变体的相对活性。通过两种催化结构域的一种，酶的截短导致受损的活性。在碱性pH值和升高盐浓度下，CBD(M23_Ami_SH3b_SH3b；SEQ ID NO:32，由SEQ ID NO；49编码)的复制加速裂解。具有CHAP11域的Ply2638改造得到以下酶，其假定会攻击在葡萄球菌属的肽聚糖层中的三种不同的键。其pH最适点改变到稍酸性条件并改善抗菌活性。然而，嵌合酶的蛋白质稳定性仍然是一个挑战。在标准条件(pH 7.4和120mM氯化钠浓度)下，Ply2638的最大的溶解速度设定为参比。棒表示重复三次测定的平均值，标准偏差未示出。(CHAP_M23_Ami_SH3b＝SEQ ID NO:25，由SEQ ID NO:45编码；Ami_M23_Ami_SH3b＝SEQ ID NO:26，由SEQ ID NO:46编码；M23_Ami_SH3b_SH3b＝SEQ ID NO:32，由SEQ ID NO:49编码；M23_Ami_SH3b＝SEQ ID NO:21，由SEQ ID NO:44编码；Ami_SH3b＝SEQ ID NO:22，由SEQ ID NO:2编码；M23_SH3b＝SEQ ID NO:23，由SEQ ID NO:3编码)。
图7在标准条件下，针对使用金黄色葡萄球菌SA2638/2854底物细胞，50nM四域酶(四重结构域酶)(SEQ ID NO:27和28，分别由SEQ ID NO:47和8编码)、Ply2638(SEQ ID NO:21，由SEQ ID NO:44编码)、和溶葡萄球菌素(SEQ ID NO:34，由SEQ ID NO:33编码)的活性。冻干缓冲液作为对照。通过结合Ply2638、PlyTw、和溶葡萄球菌素的域来构建四域酶。
实施例
材料和方法
菌株、培养条件、噬菌体和质粒
大肠杆菌XL1BlueMRF和大肠杆菌Sure用于6x-His-标记的(SEQ ID NO:43)融合蛋白的过表达。在30℃下，在LB-PE培养基中培养两种菌株，含有100μg/ml氨苄青霉素和30μg/ml四环素，用于质粒选择。噬菌体2638A裂解液作为模板，用于Ply2638A基因或其域编码区的扩增。CHAPTw域(SEQ ID NO:19)由噬菌体Twort裂解液扩增。来自噬菌体11的半胱氨酸-组氨酸依赖性酰胺酶/肽酶(CHAP)域和酰胺酶域(分别为SEQ ID NO 18和17；Donovan,etal.,2006和2008；Navarre et al.,1999；Sass and Bierbaum 2007)由包含phi11自溶素基因的pet21a载体扩增，其来自Donovan,D.M.的善意馈赠。包含成熟溶葡萄球菌素的序列(SEQ ID NO:33)的质粒LT1215作为模板，用于域扩增M23-LST(SEQ ID NO:15)和CWT-LST(SEQ ID NO:13)。
pQE-30载体(目录号：32915，Qiagen，Hilden，德国；SEQ ID NO:50)作为克隆和表达载体，用于分别在大肠杆菌XL1BlueMRF或大肠杆菌Sure中产生6x-His标记的重组融合蛋白。
DNA技术和克隆程序
按照Sambrook,Maniatis et al.(1989)的标准技术用于克隆单基因和产生融合蛋白。高保真PCR酶混合物(Fermentas)用于PCR反应。借助于分光光度计(NanoDrop ND-1000分光光度计)来确定DNA浓度。
通过将Ply2638(SEQ ID NO:1)编码序列Met1–Lys486插入pQE30(SEQ ID NO:50)部位BamHI–SalI来构建pHPl2638。pHPl2638-Pl2638构建体具有连续插入BamHI–SacI–SalI部位的相同序列。CHAP11(SEQ ID NO:18)、Ami11(SEQ ID NO:17)和CHAP_Ami11被N端引入BamHI消化的pHPL2638A。在连接反应以前，利用虾碱性磷酸酶(SAP，Fermentas)使载体去磷酸化。利用BamHI和SacI限制位点，通过用各自的插入片段替换来自pHGFP_CBD2638A_c载体(SEQ ID NO:59)的GFP编码区来构建pHM23_CBD2638(SEQ ID NO:3)和pHM23-2638_Ami2638_CBD2638_CBD2638(SEQ ID NO:49)。pHM23-LST_Ami2638_CBD2638(SEQ ID NO:48)具有pQE-30主链，其具有插入BamHI和SacI中的成熟溶葡萄球菌素(SEQ ID NO:33)编码序列Ala1-Gly154以及Ply2638部分序列，其编码在SacI和SalI位点处的Leu138-Lys486。pHM23-LST_M23-LST_CWT-LST(SEQ ID NO:11)具有插入BamHI和SacI中的成熟溶葡萄球菌素(SEQ ID NO:33)序列Ala1–Gly154以及插入pQE30的SacI和SalI位点的Ala1–Lys246。以相同的方式来构建pHLST-LST(SEQ ID NO:10)，其中在BamHI–SacI和SacI–SalI位点重复地具有Ala1–Lys246。在编码四域构建体pHCHAPTw_Ami2638_M23-LST_CBD2638(SEQ ID NO:47)和pHCHAPTw_Ami2638_M23-LST_CWT-LST(SEQ ID NO:8)的质粒中，通过剪接重叠延伸PCR(SOE)和插入pQE30BamHI和SalI位点，来直接融合域。在两种构建体中，借助于生物信息学(未发表的数据)来确定单个域的边缘区(boarder region)(CHAPTw，SEQ ID NO:19：Met1–Ile140，Ami2638,SEQ ID NO:16：SEQ ID NO:1的Lys141-Gly358，CBD2638，SEQ IDNO:12：SEQ ID NO:1的Trp393-Lys486，M23-LST，SEQ ID NO:15：SEQ ID NO:33的Ala1–Gly154，CWT-LST，SEQ ID NO:13：SEQ ID NO:33的Trp155–Lys246)。将具有重复顺序的质粒转移到大肠杆菌Sure菌株中，并将所有其它质粒转移到大肠杆菌XL1BlueMRF中。
重组融合蛋白的表达和纯化
基本上如之前由其他人(Loessner et al.,1996,Schmelcher et al.,2010)描述的，进行蛋白过表达和部分纯化。简单地说，在250ml改良LB培养基(15g/l胰蛋白，8g/l酵母提取物，5g/l NaCl，pH 7.8)中生长携带质粒的大肠杆菌，直到在600nm(OD600nm)处0.4至0.6的光密度并用1mMIPTG加以诱导。在30℃下进一步温育细胞4小时，或在20℃下温育18小时，冷却至4℃，然后通过离心作用收获。将细胞颗粒悬浮于5ml固定缓冲液(50mM NaH2PO4，500mM NaCl，5mM咪唑，0.1％聚山梨酯20，pH 7.4)中。借助于通过在1200psi下操作的弗氏压力细胞破碎器(French Pressure Cell Press)(1200psi,SLM Aminco,Urbana,IL,U.S.)的双通道来释放包含可溶性重组蛋白的细胞溶质大肠杆菌内含物。其它下游处理步骤包括通过离心作用除去不溶性细胞碎片，过滤灭菌(0.2μm PES膜，Millipore)，以及固定金属亲和层析(IMAC)纯化，其中利用填充有低密度Ni-NTA超流树脂(Chemie Brunschwig AG,Basel,Switzerland)的MicroBiospin(Bio-Rad,Hercules,CA,U.S.)柱。用5-10柱容积的固定缓冲液对Ni-NTA固定蛋白质进行柱上重力流洗涤。然后用洗脱缓冲液(50mM NaH2PO4，500mM NaCl，125mM咪唑，0.1％聚山梨酯20，pH 7.4)洗脱蛋白部分，并针对两种变化的透析缓冲液(50mM NaH2PO4，100mM NaCl，0.1％聚山梨酯20，pH 7.4)加以透析。用NanoDrop ND-1000分光光度计来确定蛋白质浓度，在280nm处校正吸收系数，如借助于VectorNTI软件(Invitrogen,Carlsbad,CA,U.S.)由一级氨基酸序列计算，并通过SDS-PAGE来估计纯度。在与50％甘油混合的情况下，将等分部分存储于-20℃。
重组蛋白的冻干
针对3次变化的300ml冻干缓冲液(50mM磷酸盐或Tris，500mM蔗糖，200mM甘露醇，pH 7.4)等分部分透析(渗析)IMAC纯化蛋白，并在液氮的气相中冷冻。在-40℃和75毫托的真空下，进行冷冻干燥60分钟，接着在5小时期间提高温度至-10℃，然后在-10℃和在相同真空水平下保持另外60分钟。作为最后的步骤，在10小时期间，将温度提高至25℃。在裂解测定中，在测试以前，通过添加水重建样品。
细胞壁结合测定
作为用来确定CBD引导GFP融合于细菌表面和介导紧密结合于细胞壁配体的能力的标准测定，使用了以下条件：通过离心作用收获来自晚对数期的细菌，优选金黄色葡萄球菌BB255，再悬浮于1/10容积的PBS-T(50mM NaH2PO4，120mM NaCl[pH 8.0]，0.01％聚山梨酯20)中，然后存储在冰上。将GFP-CBD蛋白，优选SEQ ID NO:64，由SEQ ID NO:60编码，在相同的缓冲液中稀释至400nM的浓度(2x GFP-CBD)，并同样存储在冰上。在1.5ml微型离心杯中，混合100μl细胞和100μl的2xGFP-CBD，并在室温下温育5分钟。然后通过在微型离心中的离心作用(16000g，60s)从上清液除去细胞。丢弃上清液并用500μl的PBS-T缓冲液洗涤细胞两次。对于荧光显微镜检术，最后将颗粒再悬浮于50μl缓冲液中。对于荧光计测定，最后将颗粒再悬浮于200μl的PBS-T中并转移到微量板孔中。可以利用多标记计数装置(Victor3,Perkin Elmer,Massachusetts,U.S.)并借助于无菌的未经处理的黑色96-孔聚苯乙烯微量板(Nunc,Roskilde,Denmark)进行定量荧光测定。作为阴性对照，可以使用GFP。
定量荧光测定
通过用7.5μg GFP-CBDS2638融合蛋白(SEQ ID NO:64，由SEQ ID NO:60编码)温育来自1ml容积的设定为OD600nm 1+/-0.05(约4x 109个细胞)的细胞研究pH和盐对CBD2638与金黄色葡萄球菌BB255细胞表面配体相互作用的依赖性。这种细胞蛋白比接近饱和点(如在前述实验中确定的)并使得能够检测结合效率的变化。利用柠檬酸盐缓冲液(pH 4.5至6.5)和磷酸盐缓冲液(pH 6至9)测试不同的pH值。在使用在pH缓冲液中的GFP-CBD2638蛋白温育以后，用相应的pH缓冲液洗涤细胞，接着是标准PBS-T(pH 8)洗涤。最后，将细胞调节到OD595nm＝0.3，以借助于Victor3多标记计数器检测来自其200μl悬浮液的荧光。利用借助于增加氯化钠浓度(10mM NaH2PO4，0–1000mM NaCl，0.1％聚山梨酯20，pH 6)所制备的缓冲液进行类似的实验。
通过记录洗涤和热灭活细胞(之前用过量GFP-CBD2638蛋白温育)的相对荧光单位(RFU)来测试具有改变的细胞表面特性的葡萄球菌属菌株的CBD结合能力的量化。利用在多标记计数装置中的适当的滤光片组合，来测量相同容积(200μl)的GFP-CBD2638标记细胞(调节到OD595nm＝0.3)的荧光。以相同方式，对金黄色葡萄球菌BB255细胞和SDS处理的细胞进行GFP-CBD2638(SEQ ID NO:64，由SEQ ID NO:60编码)、GFP-CBD2638-CBD2638(SEQ IDNO 65和/或66，分别由SEQ ID NO:61和62编码)、和GFP-CBD2638-CBD2638-CBD2638(SEQ IDNO:67，由SEQ ID NO:63编码)的吸收水平的比较和量化。
裂解测定
使用于裂解活性测定的底物细胞生长至在600nm(OD600)处0.4的光密度，用PBST(pH 7.4)洗涤两次，并再悬浮于包含15％甘油的PBS缓冲液(pH 7.4)中，同时将其浓缩100倍。在-20℃下存储细胞。为了进一步用于结合或裂解活性测定，融化细胞，用PBS(pH 7.4)洗涤，并稀释至1±0.05的OD600。在标准裂解活性测定中，将蛋白样品稀释至等摩尔量并分布于透明的96-孔组织培养试验板(SPL life sciences，Pocheon，Korea)。加入底物细胞至最终体积并在37℃下记录在595nm(OD595nm)处的光密度降低约1小时。
在裂解测定中，针对来自冷冻储备液的噬菌体2638A繁殖株金黄色葡萄球菌SA2638/2854，测试Ply2638的改造和检测构建体的裂解活性。我们测试了在各种缓冲液条件下的活性。利用柠檬酸盐/磷酸盐缓冲液(25mM柠檬酸盐、25mM磷酸盐、120mM NaCl、pH4.6-6.6)和Tris/磷酸盐缓冲液(25mM Tris、25mM磷酸盐、120mM NaCl)，测试了增量为0.4的从4.6至9的pH值。在0至1000mM氯化钠(在10mM磷酸盐缓冲液中，pH 7.4)的盐浓度下，测试了Ply2638A衍生物的活性。在裂解测定中，在其施加之前，用MQ将Ply2638A衍生物稀释至10μM的最终浓度。此处，利用多通道移液管，将4μl的10μM Ply2638A衍生物施加于196μl基底细胞悬浮液，从而得到200nM蛋白的测定浓度。基底细胞悬浮液制备自冷冻储备液：用pH或盐缓冲液将其稀释并用分光光度法(Libra S22,Biochrom)将其标准化至1±0.05的初始OD600。在1小时期间，利用Victor31420多标记分析仪(Multilabel Counter instrument)(Perkin Elmer)来测量在595nm(OD595)处光密度的降低。在每个单读数以后，剧烈摇晃平板1秒(双轨道，0.1mm直径)。作为阳性对照，使用N端6xHis标记的溶葡萄球菌素(HLST)，市售溶葡萄球菌素(重组，源自大肠杆菌，Sigma)。作为阴性对照，使用MilliQ水。
二价金属离子对Ply2638的活性的影响
针对包含EDTA的缓冲液(50mM MOPS、100mM氯化钠、0.005％聚山梨酯20、10mMEDTA)透析部分纯化的Ply2638(SEQ ID NO:21)2小时，接着针对包含相应的二价金属离子的缓冲液(50mM MOPS，100mM氯化钠，0.005％聚山梨酯20，以及分别10mM CaCl2、10mMMgCl2、1mM CoCl2、1mM CuCl2、1mM MnSO4、或1mM ZnCl2)进行透析。在用于标准裂解测定中之前，SDS处理和EDTA洗涤用作底物的细胞。
表1.SEQ ID NO识别
SEQ ID NO:1(Ply2638)
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：2(Ami2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：3(M23-2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：4(Ply2638-Plv2638+6x His和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：5(CHAP11_M23-2638_Ami2638_CBD2638)
ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：6(Ami11_M23-2638_Ami2638_CBD2638)
AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：7(CHAPTw_Ami2638_M23-LST_CBD2638)
ATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：8(CHAPTw_Ami2638_M23-LST_CWT-LST+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTAA
SEQ ID NO：9(M23-LST_Ami2638_CBD2638)
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：10(LST_LST+6xHis和克降位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGGAGCTCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
SEQ ID NO：11(M23-LST_M23-LST_CWT-LST+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
SEQ ID NO：12(CBD-2638)
TGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：13(CWT-LST)
TGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
SEQ ID NO：14(M23-2638)
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGAT
SEQ ID NO：15(M23-LST)
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATAT
SEQ ID NO：16(Ami-2638)
GGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGT
SEQ ID NO：17(Ami-11)
AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCA
SEQ ID NO：18(CHAP-I1)
ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCT
SEQ ID NO：19(CHAP-Twort)
ATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATT
SEQ ID NO：20(M23-2638_Ami2638_CBD2638_CBD2638)
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：21(Plv2638)
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：22(Ami2638_CBD2638)
MRGSHHHHHHGSLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：23(M23-2638_CBD2638)
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：24(Plv2638-Plv2638)
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：25(CHAP11_M23-2638_Ami2638_CBD2638)
MRGSHHHHHHGSMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：26(Ami11_M23-2638_Ami2638_CBD2638)
MRGSHHHHHHGSKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：27(CHAPTw_Ami2638_M23-LST_CBD2638)
MRGSHHHHHHGSMKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：28(CHAPTw_Ami2638_M23-LST_CWT-LST)
MRGSHHHHHHGSMKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
SEQ ID NO：29(M23-LST_Ami2638_CBD2638)
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGELLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：30(LST LST)
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIKELAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
SEQ ID NO：31(M23-LST_M23-LST_CWT-LST)
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGELAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
SEQ ID NO：32(M23-2638_Ami2638_CBD2638_CBD2638)
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：33(LST)
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
SEQ ID NO：34(LST aa)
AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
SEQ ID NO：35(CBD-2638)
WKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：36(CWT-LST)
WKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
SEQ ID NO：37(M23-2638)
MLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKD
SEQ ID NO：38(M23-LST)
AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGY
SEQ ID NO：39(Ami-2638)
NKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDG
SEQ ID NO：40(Ami-11)
NPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMD
SEQ ID NO：41(CHAP-11)
MSIIMEVATMQAKLTKNEF1EWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRP
SEQ ID NO：42(CHAP-Twort)
MKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVWWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFI
SEQ ID NO 43(6xHis标记N端)
MRGSHHHHHHGS
SEQ ID NO：44(Plv2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：45(CHAP11_M23-2638_Ami2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：46(Ami11_M23-2638_Ami2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCAAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：47(CHAPTw_Ami2638_M23-LST_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：48(M23-LST_Ami2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：49(M23-2638_Ami2638_CBD2638_CBD2638+6xHis和克隆位点)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：50(pQE-30载体，可获自登录号：32915，Qiagen-Hilden，德国)
CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCGCATGCGAGCTCGGTACCCCGGGTCGACCTGCAGCCAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC
SEQ ID NO：51(CBD-2638，具有以粗体表示的假定接头)
SEQ ID NO：52(CWT-LST，具有以粗体表示的假定接头)
SEQ ID NO：53(M23-2638，具有以粗体表示的假定接头)
SEQ ID NO：54(M23-LST，具有以粗体表示的假定接头)
SEQ ID NO：55(Ami-2638，具有以粗体表示的假定接头)
SEQ ID NO：56(Ami-11，具有以粗体表示的假定接头)
SEQ ID NO：57(CHAP-11，具有以粗体表示的假定接头)
SEQ ID NO：58(CHAP-Twort，具有以粗体表示的假定接头)
SEQ ID NO：59(pHGFP_CBD2638_c载体)
CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAAGTCGACCTGCAGCCAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC
SEQ ID NO：60(GFP_CBD2638)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
SEQ ID NO：61(GFP_CBD2638_CBD2638变体1。限制位点，用于构建：源自第二 CBD2638的BamHI-SacI-SacI-SalI.TAA终止密码子)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAAGTCGAC
SEQ ID NO：62(GFP_CBD2638_CBD2638变体2。限制位点，用于构建：由载体编码的 BamHI-SacI-KpnI-SalI终止密码子)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGGTACCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGTCGACCTGCAGCCAAGCTTAATTAGCTGA
SEQ ID NO：63(GFP_CBD2638_CBD2638_CBD2638。限制位点，用于构建：由载体编码 的BamHI-SacI-KpnI-SalI-PstI.终止密码子)
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGGTACCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGTCGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAACTGCAGCCAAGCTTAATTAGCTGA
SEQ ID NO：64(GFP_CBD2638 aa)
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：65(GFP_CBD2638_CBD2638变体1 aa)
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
SEQ ID NO：66(GFP_CBD2638_CBD2638变体2 aa)
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDLQPSLIS
SEQ ID NO：67(GFP_CBD2638_CBD2638_CBD2638aa)
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKLQPSLIS。
序列表
<110> 迈克瑞欧斯人体健康有限公司
<120> 一种多肽
<130> P6035159PCT
<160> 67
<170> PatentIn version 3.3
<210> 1
<211> 1461
<212> DNA
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 1
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60
acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120
tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180
gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240
ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300
ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360
ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420
aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480
gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540
aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600
caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660
ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720
agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780
tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840
gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900
tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960
tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020
aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080
aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140
aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 1200
tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 1260
tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 1320
aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 1380
ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 1440
ttgtggggcg aaattaaata a 1461
<210> 2
<211> 1086
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的Ami2638_CBD2368
<400> 2
atgagaggat cgcatcacca tcaccatcac ggatccttac gccctaaaga cgcaaagaaa 60
gatgaaaaat cacaagtatg tagtggtttg gctatggaaa aatatgacat tacaaattta 120
aatgctaaac aagataaatc aaagaatggg agcgtgaaag agttgaaaca tatctattca 180
aaccatatta aaggtaacaa gattacagca ccaaaaccta gtattcaagg tgtggtcatc 240
cacaatgatt atggtagtat gacacctagt caatacttac catggttata tgcacgtgag 300
aataacggta cacacgttaa cggttgggct agtgtttatg caaatagaaa cgaagtgctt 360
tggtatcatc cgacagacta cgtagagtgg cattgtggta atcaatgggc aaatgctaac 420
ttaatcggat ttgaagtgtg tgagtcgtat cctggtagaa tctcggacaa attattctta 480
gaaaatgaag aagcgacatt gaaagtagct gcggatgtga tgaagtcgta cggattacca 540
gttaatcgca acactgtacg tctgcataac gaattcttcg gaacttcttg tccacatcgt 600
tcgtgggact tgcatgttgg caaaggtgag ccttacacaa ctactaatat taataaaatg 660
aaagactact tcatcaaacg catcaaacat tattatgacg gtggaaagct agaagtaagc 720
aaagcagcaa ctatcaaaca atctgacgtt aagcaagaag ttaaaaagca agaagcaaaa 780
caaattgtga aagcaacaga ttggaaacag aataaagatg gcatttggta taaagctgaa 840
catgcttcgt tcacagtgac agcaccagag ggaattatca caagatacaa aggtccttgg 900
actggtcacc cacaagctgg tgtattacaa aaaggtcaaa cgattaaata tgatgaggtt 960
caaaaatttg acggtcatgt ttgggtatcg tgggaaacgt ttgagggcga aactgtatac 1020
atgccggtac gcacatggga cgctaaaact ggtaaagttg gtaagttgtg gggcgaaatt 1080
aaataa 1086
<210> 3
<211> 1035
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的M23-2638_CBD2638
<400> 3
atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60
acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120
tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180
catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240
acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300
aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360
gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420
cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgagctcgg tggaaagcta 660
gaagtaagca aagcagcaac tatcaaacaa tctgacgtta agcaagaagt taaaaagcaa 720
gaagcaaaac aaattgtgaa agcaacagat tggaaacaga ataaagatgg catttggtat 780
aaagctgaac atgcttcgtt cacagtgaca gcaccagagg gaattatcac aagatacaaa 840
ggtccttgga ctggtcaccc acaagctggt gtattacaaa aaggtcaaac gattaaatat 900
gatgaggttc aaaaatttga cggtcatgtt tgggtatcgt gggaaacgtt tgagggcgaa 960
actgtataca tgccggtacg cacatgggac gctaaaactg gtaaagttgg taagttgtgg 1020
ggcgaaatta aataa 1035
<210> 4
<211> 2961
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的Ply2638_Ply2638
<400> 4
atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60
acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120
tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180
catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240
acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300
aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360
gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420
cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660
tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720
acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780
ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840
tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900
gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960
aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020
ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080
ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200
aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260
ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320
ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380
gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440
cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaagagctc 1500
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 1560
acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 1620
tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 1680
gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 1740
ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 1800
ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 1860
ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 1920
aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 1980
gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 2040
aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 2100
caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 2160
ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 2220
agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 2280
tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 2340
gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 2400
tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 2460
tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 2520
aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 2580
aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 2640
aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 2700
tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 2760
tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 2820
aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 2880
ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 2940
ttgtggggcg aaattaaata a 2961
<210> 5
<211> 1983
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> CHAP11_M23-2638_Ami2638_CBD2638
<400> 5
atgcaagcaa aattaactaa aaatgagttt atagagtggt tgaaaacttc tgagggaaaa 60
caattcaatg tggacttatg gtatggattt caatgctttg attatgccaa tgctggttgg 120
aaagttttgt ttggattact tctaaaaggt ttaggtgcaa aagatattcc gttcgctaac 180
aacttcgacg gattagctac tgtataccaa aatacaccgg acttcttagc acaacctggc 240
gacatggtgg tattcggtag caactacggt gctggatatg gtcacgttgc atgggtaatt 300
gaagcaactt tagattacat cattgtatat gagcagaatt ggctaggcgg tggctggact 360
gacggaatcg aacaacccgg ctggggttgg gaaaaagtta caagacgaca acatgcttat 420
gatttcccta tgtggtttat ccgtccgaat tttaaaagtg agacagcgcc acgatcagtt 480
caatctccta cacaagcacc taaaaaagaa acagctggat ccatgctaac tgctattgac 540
tatcttacga aaaaaggttg gaaaatatca tctgaccctc gcacttacga tggttaccct 600
aaaaactacg gctacagaaa ttaccatgaa aacggcatta attatgatga gttttgtggt 660
ggttatcata gagcttttga tgtttacagt aacgaaacta acgacgtgcc tgctgttact 720
agcggaacag ttattgaagc aaacgattac ggtaattttg gtggtacatt cgttattaga 780
gacgctaacg ataacgattg gatatatggg catctacaac gtggctcaat gcgatttgtt 840
gtaggcgaca aagtcaatca aggtgacatt attggtttac aaggtaatag caactattac 900
gacaatccta tgagtgtaca tttacattta caattacgcc ctaaagacgc aaagaaagat 960
gaaaaatcac aagtatgtag tggtttggct atggaaaaat atgacattac aaatttaaat 1020
gctaaacaag ataaatcaaa gaatgggagc gtgaaagagt tgaaacatat ctattcaaac 1080
catattaaag gtaacaagat tacagcacca aaacctagta ttcaaggtgt ggtcatccac 1140
aatgattatg gtagtatgac acctagtcaa tacttaccat ggttatatgc acgtgagaat 1200
aacggtacac acgttaacgg ttgggctagt gtttatgcaa atagaaacga agtgctttgg 1260
tatcatccga cagactacgt agagtggcat tgtggtaatc aatgggcaaa tgctaactta 1320
atcggatttg aagtgtgtga gtcgtatcct ggtagaatct cggacaaatt attcttagaa 1380
aatgaagaag cgacattgaa agtagctgcg gatgtgatga agtcgtacgg attaccagtt 1440
aatcgcaaca ctgtacgtct gcataacgaa ttcttcggaa cttcttgtcc acatcgttcg 1500
tgggacttgc atgttggcaa aggtgagcct tacacaacta ctaatattaa taaaatgaaa 1560
gactacttca tcaaacgcat caaacattat tatgacggtg gaaagctaga agtaagcaaa 1620
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 1680
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 1740
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1800
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1860
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1920
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1980
taa 1983
<210> 6
<211> 2109
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> Ami11_M23-2638_Ami2638_CBD2638
<400> 6
aagccacaac ctaaagcagt agaacttaaa atcatcaaag atgtggttaa aggttatgac 60
ctacctaagc gtggtagtaa ccctaaaggt atagttatac acaacgacgc agggagcaaa 120
ggggcgactg ctgaagcata tcgtaacgga ttagtaaatg cacctttatc aagattagaa 180
gcgggcattg cgcatagtta cgtatcaggc aacacagttt ggcaagcctt agatgaatca 240
caagtaggtt ggcataccgc taatcaaata ggtaataaat attattacgg tattgaagta 300
tgtcaatcaa tgggcgcaga taacgcgaca ttcttaaaaa atgaacaggc aactttccaa 360
gaatgcgcta gattgttgaa aaaatgggga ttaccagcaa acagaaatac aatcagattg 420
cacaatgaat ttacttcaac atcatgccct catagaagtt cggttttaca cactggtttt 480
gacccagtaa ctcgcggtct attgccagaa gacaagcggt tgcaacttaa agactacttt 540
atcaagcaga ttagggcgta catggatggt aaaataccgg ttgccactgt ctctaatgag 600
tcaagcgctt caagtaatac agttaaacca gttgcaagtg caggatccat gctaactgct 660
attgactatc ttacgaaaaa aggttggaaa atatcatctg accctcgcac ttacgatggt 720
taccctaaaa actacggcta cagaaattac catgaaaacg gcattaatta tgatgagttt 780
tgtggtggtt atcatagagc ttttgatgtt tacagtaacg aaactaacga cgtgcctgct 840
gttactagcg gaacagttat tgaagcaaac gattacggta attttggtgg tacattcgtt 900
attagagacg ctaacgataa cgattggata tatgggcatc tacaacgtgg ctcaatgcga 960
tttgttgtag gcgacaaagt caatcaaggt gacattattg gtttacaagg taatagcaac 1020
tattacgaca atcctatgag tgtacattta catttacaat tacgccctaa agacgcaaag 1080
aaagatgaaa aatcacaagt atgtagtggt ttggctatgg aaaaatatga cattacaaat 1140
ttaaatgcta aacaagataa atcaaagaat gggagcgtga aagagttgaa acatatctat 1200
tcaaaccata ttaaaggtaa caagattaca gcaccaaaac ctagtattca aggtgtggtc 1260
atccacaatg attatggtag tatgacacct agtcaatact taccatggtt atatgcacgt 1320
gagaataacg gtacacacgt taacggttgg gctagtgttt atgcaaatag aaacgaagtg 1380
ctttggtatc atccgacaga ctacgtagag tggcattgtg gtaatcaatg ggcaaatgct 1440
aacttaatcg gatttgaagt gtgtgagtcg tatcctggta gaatctcgga caaattattc 1500
ttagaaaatg aagaagcgac attgaaagta gctgcggatg tgatgaagtc gtacggatta 1560
ccagttaatc gcaacactgt acgtctgcat aacgaattct tcggaacttc ttgtccacat 1620
cgttcgtggg acttgcatgt tggcaaaggt gagccttaca caactactaa tattaataaa 1680
atgaaagact acttcatcaa acgcatcaaa cattattatg acggtggaaa gctagaagta 1740
agcaaagcag caactatcaa acaatctgac gttaagcaag aagttaaaaa gcaagaagca 1800
aaacaaattg tgaaagcaac agattggaaa cagaataaag atggcatttg gtataaagct 1860
gaacatgctt cgttcacagt gacagcacca gagggaatta tcacaagata caaaggtcct 1920
tggactggtc acccacaagc tggtgtatta caaaaaggtc aaacgattaa atatgatgag 1980
gttcaaaaat ttgacggtca tgtttgggta tcgtgggaaa cgtttgaggg cgaaactgta 2040
tacatgccgg tacgcacatg ggacgctaaa actggtaaag ttggtaagtt gtggggcgaa 2100
attaaataa 2109
<210> 7
<211> 1923
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> CHAPTw_Ami2638_M23-LST_CBD2638
<400> 7
atgaaaaccc tgaaacaagc agagtcctac attaagagta aagtaaatac aggaactgat 60
tttgatggtt tatatgggta tcagtgtatg gacttagcag tagattatat ttaccatgta 120
acagatggta aaataagaat gtggggtaat gctaaggatg cgataaataa ctcttttggt 180
ggtactgcta cggtatataa aaactaccct gcttttagac ctaagtacgg tgatgtagtc 240
gtatggacta ctggtaattt tgcaacttat ggtcatatcg caatagttac taaccctgac 300
ccttatggag accttcaata tgttacagtt cttgaacaaa actggaacgg taacgggatt 360
tataaaaccg agttagctac aatcagaaca cacgattaca caggaattac acattttatt 420
aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480
gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540
aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600
caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660
ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720
agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780
tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840
gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900
tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960
tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020
aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080
aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140
aagcaagaag caaaacaaat tgtgaaagca acagatgctg caacacatga acattcagca 1200
caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 1260
ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 1320
tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 1380
cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 1440
aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 1500
tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 1560
caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 1620
ccaacgccga atacaggttg gaaacagaat aaagatggca tttggtataa agctgaacat 1680
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1740
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1800
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1860
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1920
taa 1923
<210> 8
<211> 1953
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的CHAPTw_Ami2638_M23-LST_CWT-LST
<400> 8
atgagaggat cgcatcacca tcaccatcac ggatccatga aaaccctgaa acaagcagag 60
tcctacatta agagtaaagt aaatacagga actgattttg atggtttata tgggtatcag 120
tgtatggact tagcagtaga ttatatttac catgtaacag atggtaaaat aagaatgtgg 180
ggtaatgcta aggatgcgat aaataactct tttggtggta ctgctacggt atataaaaac 240
taccctgctt ttagacctaa gtacggtgat gtagtcgtat ggactactgg taattttgca 300
acttatggtc atatcgcaat agttactaac cctgaccctt atggagacct tcaatatgtt 360
acagttcttg aacaaaactg gaacggtaac gggatttata aaaccgagtt agctacaatc 420
agaacacacg attacacagg aattacacat tttattaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660
tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720
acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780
ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840
tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900
gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960
aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020
ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080
ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200
aaagcaacag atgctgcaac acatgaacat tcagcacaat ggttgaataa ttacaaaaaa 1260
ggatatggtt acggtcctta tccattaggt ataaatggcg gtatgcacta cggagttgat 1320
ttttttatga atattggaac accagtaaaa gctatttcaa gcggaaaaat agttgaagct 1380
ggttggagta attacggagg aggtaatcaa ataggtctta ttgaaaatga tggagtgcat 1440
agacaatggt atatgcatct aagtaaatat aatgttaaag taggagatta tgtcaaagct 1500
ggtcaaataa tcggttggtc tggaagcact ggttattcta cagcaccaca tttacacttc 1560
caaagaatgg ttaattcatt ttcaaattca actgcccaag atccaatgcc tttcttaaag 1620
agcgcaggat atggaaaagc aggtggtaca gtaactccaa cgccgaatac aggttggaaa 1680
acaaacaaat atggcacact atataaatca gagtcagcta gcttcacacc taatacagat 1740
ataataacaa gaacgactgg tccatttaga agcatgccgc agtcaggagt cttaaaagca 1800
ggtcaaacaa ttcattatga tgaagtgatg aaacaagacg gtcatgtttg ggtaggttat 1860
acaggtaaca gtggccaacg tatttacttg cctgtaagaa catggaataa atctactaat 1920
actttaggtg ttctttgggg aactataaag taa 1953
<210> 9
<211> 1518
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> M23-LST_Ami2638_CBD2638
<400> 9
gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60
ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120
attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180
tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240
atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300
ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360
aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420
ggaaaagcag gtggtacagt aactccaacg ccgaatacag gtgagctctt acgccctaaa 480
gacgcaaaga aagatgaaaa atcacaagta tgtagtggtt tggctatgga aaaatatgac 540
attacaaatt taaatgctaa acaagataaa tcaaagaatg ggagcgtgaa agagttgaaa 600
catatctatt caaaccatat taaaggtaac aagattacag caccaaaacc tagtattcaa 660
ggtgtggtca tccacaatga ttatggtagt atgacaccta gtcaatactt accatggtta 720
tatgcacgtg agaataacgg tacacacgtt aacggttggg ctagtgttta tgcaaataga 780
aacgaagtgc tttggtatca tccgacagac tacgtagagt ggcattgtgg taatcaatgg 840
gcaaatgcta acttaatcgg atttgaagtg tgtgagtcgt atcctggtag aatctcggac 900
aaattattct tagaaaatga agaagcgaca ttgaaagtag ctgcggatgt gatgaagtcg 960
tacggattac cagttaatcg caacactgta cgtctgcata acgaattctt cggaacttct 1020
tgtccacatc gttcgtggga cttgcatgtt ggcaaaggtg agccttacac aactactaat 1080
attaataaaa tgaaagacta cttcatcaaa cgcatcaaac attattatga cggtggaaag 1140
ctagaagtaa gcaaagcagc aactatcaaa caatctgacg ttaagcaaga agttaaaaag 1200
caagaagcaa aacaaattgt gaaagcaaca gattggaaac agaataaaga tggcatttgg 1260
tataaagctg aacatgcttc gttcacagtg acagcaccag agggaattat cacaagatac 1320
aaaggtcctt ggactggtca cccacaagct ggtgtattac aaaaaggtca aacgattaaa 1380
tatgatgagg ttcaaaaatt tgacggtcat gtttgggtat cgtgggaaac gtttgagggc 1440
gaaactgtat acatgccggt acgcacatgg gacgctaaaa ctggtaaagt tggtaagttg 1500
tggggcgaaa ttaaataa 1518
<210> 10
<211> 1521
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的LST_LST
<400> 10
atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60
caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120
ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180
tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240
cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300
aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360
tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420
caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480
ccaacgccga atacaggttg gaaaacaaac aaatatggca cactatataa atcagagtca 540
gctagcttca cacctaatac agatataata acaagaacga ctggtccatt tagaagcatg 600
ccgcagtcag gagtcttaaa agcaggtcaa acaattcatt atgatgaagt gatgaaacaa 660
gacggtcatg tttgggtagg ttatacaggt aacagtggcc aacgtattta cttgcctgta 720
agaacatgga ataaatctac taatacttta ggtgttcttt ggggaactat aaaggagctc 780
gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 840
ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 900
attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 960
tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 1020
atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 1080
ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 1140
aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 1200
ggaaaagcag gtggtacagt aactccaacg ccgaatacag gttggaaaac aaacaaatat 1260
ggcacactat ataaatcaga gtcagctagc ttcacaccta atacagatat aataacaaga 1320
acgactggtc catttagaag catgccgcag tcaggagtct taaaagcagg tcaaacaatt 1380
cattatgatg aagtgatgaa acaagacggt catgtttggg taggttatac aggtaacagt 1440
ggccaacgta tttacttgcc tgtaagaaca tggaataaat ctactaatac tttaggtgtt 1500
ctttggggaa ctataaagtg a 1521
<210> 11
<211> 1245
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的M23-LST_M23_LST-CWT-LST
<400> 11
atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60
caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120
ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180
tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240
cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300
aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360
tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420
caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480
ccaacgccga atacaggtga gctcgctgca acacatgaac attcagcaca atggttgaat 540
aattacaaaa aaggatatgg ttacggtcct tatccattag gtataaatgg cggtatgcac 600
tacggagttg atttttttat gaatattgga acaccagtaa aagctatttc aagcggaaaa 660
atagttgaag ctggttggag taattacgga ggaggtaatc aaataggtct tattgaaaat 720
gatggagtgc atagacaatg gtatatgcat ctaagtaaat ataatgttaa agtaggagat 780
tatgtcaaag ctggtcaaat aatcggttgg tctggaagca ctggttattc tacagcacca 840
catttacact tccaaagaat ggttaattca ttttcaaatt caactgccca agatccaatg 900
cctttcttaa agagcgcagg atatggaaaa gcaggtggta cagtaactcc aacgccgaat 960
acaggttgga aaacaaacaa atatggcaca ctatataaat cagagtcagc tagcttcaca 1020
cctaatacag atataataac aagaacgact ggtccattta gaagcatgcc gcagtcagga 1080
gtcttaaaag caggtcaaac aattcattat gatgaagtga tgaaacaaga cggtcatgtt 1140
tgggtaggtt atacaggtaa cagtggccaa cgtatttact tgcctgtaag aacatggaat 1200
aaatctacta atactttagg tgttctttgg ggaactataa agtga 1245
<210> 12
<211> 285
<212> DNA
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 12
tggaaacaga ataaagatgg catttggtat aaagctgaac atgcttcgtt cacagtgaca 60
gcaccagagg gaattatcac aagatacaaa ggtccttgga ctggtcaccc acaagctggt 120
gtattacaaa aaggtcaaac gattaaatat gatgaggttc aaaaatttga cggtcatgtt 180
tgggtatcgt gggaaacgtt tgagggcgaa actgtataca tgccggtacg cacatgggac 240
gctaaaactg gtaaagttgg taagttgtgg ggcgaaatta aataa 285
<210> 13
<211> 279
<212> DNA
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 13
tggaaaacaa acaaatatgg cacactatat aaatcagagt cagctagctt cacacctaat 60
acagatataa taacaagaac gactggtcca tttagaagca tgccgcagtc aggagtctta 120
aaagcaggtc aaacaattca ttatgatgaa gtgatgaaac aagacggtca tgtttgggta 180
ggttatacag gtaacagtgg ccaacgtatt tacttgcctg taagaacatg gaataaatct 240
actaatactt taggtgttct ttggggaact ataaagtga 279
<210> 14
<211> 438
<212> DNA
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 14
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60
acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120
tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180
gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240
ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300
ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360
ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420
aaagacgcaa agaaagat 438
<210> 15
<211> 420
<212> DNA
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 15
gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60
ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120
attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180
tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240
atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300
ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360
aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420
<210> 16
<211> 510
<212> DNA
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 16
ggtaacaaga ttacagcacc aaaacctagt attcaaggtg tggtcatcca caatgattat 60
ggtagtatga cacctagtca atacttacca tggttatatg cacgtgagaa taacggtaca 120
cacgttaacg gttgggctag tgtttatgca aatagaaacg aagtgctttg gtatcatccg 180
acagactacg tagagtggca ttgtggtaat caatgggcaa atgctaactt aatcggattt 240
gaagtgtgtg agtcgtatcc tggtagaatc tcggacaaat tattcttaga aaatgaagaa 300
gcgacattga aagtagctgc ggatgtgatg aagtcgtacg gattaccagt taatcgcaac 360
actgtacgtc tgcataacga attcttcgga acttcttgtc cacatcgttc gtgggacttg 420
catgttggca aaggtgagcc ttacacaact actaatatta ataaaatgaa agactacttc 480
atcaaacgca tcaaacatta ttatgacggt 510
<210> 17
<211> 642
<212> DNA
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 17
aagccacaac ctaaagcagt agaacttaaa atcatcaaag atgtggttaa aggttatgac 60
ctacctaagc gtggtagtaa ccctaaaggt atagttatac acaacgacgc agggagcaaa 120
ggggcgactg ctgaagcata tcgtaacgga ttagtaaatg cacctttatc aagattagaa 180
gcgggcattg cgcatagtta cgtatcaggc aacacagttt ggcaagcctt agatgaatca 240
caagtaggtt ggcataccgc taatcaaata ggtaataaat attattacgg tattgaagta 300
tgtcaatcaa tgggcgcaga taacgcgaca ttcttaaaaa atgaacaggc aactttccaa 360
gaatgcgcta gattgttgaa aaaatgggga ttaccagcaa acagaaatac aatcagattg 420
cacaatgaat ttacttcaac atcatgccct catagaagtt cggttttaca cactggtttt 480
gacccagtaa ctcgcggtct attgccagaa gacaagcggt tgcaacttaa agactacttt 540
atcaagcaga ttagggcgta catggatggt aaaataccgg ttgccactgt ctctaatgag 600
tcaagcgctt caagtaatac agttaaacca gttgcaagtg ca 642
<210> 18
<211> 516
<212> DNA
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 18
atgcaagcaa aattaactaa aaatgagttt atagagtggt tgaaaacttc tgagggaaaa 60
caattcaatg tggacttatg gtatggattt caatgctttg attatgccaa tgctggttgg 120
aaagttttgt ttggattact tctaaaaggt ttaggtgcaa aagatattcc gttcgctaac 180
aacttcgacg gattagctac tgtataccaa aatacaccgg acttcttagc acaacctggc 240
gacatggtgg tattcggtag caactacggt gctggatatg gtcacgttgc atgggtaatt 300
gaagcaactt tagattacat cattgtatat gagcagaatt ggctaggcgg tggctggact 360
gacggaatcg aacaacccgg ctggggttgg gaaaaagtta caagacgaca acatgcttat 420
gatttcccta tgtggtttat ccgtccgaat tttaaaagtg agacagcgcc acgatcagtt 480
caatctccta cacaagcacc taaaaaagaa acagct 516
<210> 19
<211> 420
<212> DNA
<213> 葡萄球菌噬菌体Twort（Staphylococcus phage Twort）
<400> 19
atgaaaaccc tgaaacaagc agagtcctac attaagagta aagtaaatac aggaactgat 60
tttgatggtt tatatgggta tcagtgtatg gacttagcag tagattatat ttaccatgta 120
acagatggta aaataagaat gtggggtaat gctaaggatg cgataaataa ctcttttggt 180
ggtactgcta cggtatataa aaactaccct gcttttagac ctaagtacgg tgatgtagtc 240
gtatggacta ctggtaattt tgcaacttat ggtcatatcg caatagttac taaccctgac 300
ccttatggag accttcaata tgttacagtt cttgaacaaa actggaacgg taacgggatt 360
tataaaaccg agttagctac aatcagaaca cacgattaca caggaattac acattttatt 420
<210> 20
<211> 1851
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> M23-2638_Ami2638_CBD2638_CBD2638
<400> 20
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60
acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120
tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180
gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240
ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300
ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360
ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420
aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480
gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540
aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600
caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660
ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720
agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780
tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840
gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900
tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960
tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020
aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080
aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140
aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 1200
tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 1260
tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 1320
aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 1380
ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 1440
ttgtggggcg aaattaaaga gctcggtgga aagctagaag taagcaaagc agcaactatc 1500
aaacaatctg acgttaagca agaagttaaa aagcaagaag caaaacaaat tgtgaaagca 1560
acagattgga aacagaataa agatggcatt tggtataaag ctgaacatgc ttcgttcaca 1620
gtgacagcac cagagggaat tatcacaaga tacaaaggtc cttggactgg tcacccacaa 1680
gctggtgtat tacaaaaagg tcaaacgatt aaatatgatg aggttcaaaa atttgacggt 1740
catgtttggg tatcgtggga aacgtttgag ggcgaaactg tatacatgcc ggtacgcaca 1800
tgggacgcta aaactggtaa agttggtaag ttgtggggcg aaattaaata a 1851
<210> 21
<211> 498
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的Ply2638
<400> 21
Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala
1 5 10 15
Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg
20 25 30
Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu
35 40 45
Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe
50 55 60
Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly
65 70 75 80
Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val
85 90 95
Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gln Arg
100 105 110
Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gln Gly Asp Ile
115 120 125
Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val
130 135 140
His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met
210 215 220
Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly
225 230 235 240
Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val
245 250 255
Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln
260 265 270
Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro
275 280 285
Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu
290 295 300
Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg
305 310 315 320
Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His
325 330 335
Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr
340 345 350
Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr
355 360 365
Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln
370 375 380
Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val
385 390 395 400
Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala
405 410 415
Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg
420 425 430
Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys
435 440 445
Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val
450 455 460
Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val
465 470 475 480
Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu
485 490 495
Ile Lys
<210> 22
<211> 361
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的Ami2638_CBD2638
<400> 22
Met Arg Gly Ser His His His His His His Gly Ser Leu Arg Pro Lys
1 5 10 15
Asp Ala Lys Lys Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala Met
20 25 30
Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser Lys
35 40 45
Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys
50 55 60
Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val Ile
65 70 75 80
His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu
85 90 95
Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val
100 105 110
Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val
115 120 125
Glu Trp His Cys Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe
130 135 140
Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu
145 150 155 160
Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser
165 170 175
Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe
180 185 190
Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys
195 200 205
Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe
210 215 220
Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser
225 230 235 240
Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys
245 250 255
Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn Lys
260 265 270
Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala
275 280 285
Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro
290 295 300
Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val
305 310 315 320
Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly
325 330 335
Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys
340 345 350
Val Gly Lys Leu Trp Gly Glu Ile Lys
355 360
<210> 23
<211> 344
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的M23-2638_CBD2638
<400> 23
Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala
1 5 10 15
Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg
20 25 30
Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu
35 40 45
Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe
50 55 60
Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly
65 70 75 80
Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val
85 90 95
Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gln Arg
100 105 110
Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gln Gly Asp Ile
115 120 125
Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val
130 135 140
His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Glu Leu Gly Gly Lys Leu Glu Val Ser Lys
210 215 220
Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln
225 230 235 240
Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp
245 250 255
Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro
260 265 270
Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gln
275 280 285
Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln
290 295 300
Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu
305 310 315 320
Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val
325 330 335
Gly Lys Leu Trp Gly Glu Ile Lys
340
<210> 24
<211> 986
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的Ply2638_Ply2638
<400> 24
Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala
1 5 10 15
Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg
20 25 30
Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu
35 40 45
Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe
50 55 60
Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly
65 70 75 80
Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val
85 90 95
Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gln Arg
100 105 110
Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gln Gly Asp Ile
115 120 125
Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val
130 135 140
His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met
210 215 220
Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly
225 230 235 240
Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val
245 250 255
Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln
260 265 270
Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro
275 280 285
Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu
290 295 300
Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg
305 310 315 320
Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His
325 330 335
Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr
340 345 350
Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr
355 360 365
Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln
370 375 380
Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val
385 390 395 400
Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala
405 410 415
Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg
420 425 430
Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys
435 440 445
Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val
450 455 460
Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val
465 470 475 480
Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu
485 490 495
Ile Lys Glu Leu Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly
500 505 510
Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn
515 520 525
Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe
530 535 540
Cys Gly Gly Tyr His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn
545 550 555 560
Asp Val Pro Ala Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr
565 570 575
Gly Asn Phe Gly Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp
580 585 590
Trp Ile Tyr Gly His Leu Gln Arg Gly Ser Met Arg Phe Val Val Gly
595 600 605
Asp Lys Val Asn Gln Gly Asp Ile Ile Gly Leu Gln Gly Asn Ser Asn
610 615 620
Tyr Tyr Asp Asn Pro Met Ser Val His Leu His Leu Gln Leu Arg Pro
625 630 635 640
Lys Asp Ala Lys Lys Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala
645 650 655
Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser
660 665 670
Lys Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile
675 680 685
Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val
690 695 700
Ile His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp
705 710 715 720
Leu Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser
725 730 735
Val Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr
740 745 750
Val Glu Trp His Cys Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly
755 760 765
Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe
770 775 780
Leu Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys
785 790 795 800
Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu
805 810 815
Phe Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly
820 825 830
Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr
835 840 845
Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val
850 855 860
Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys
865 870 875 880
Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn
885 890 895
Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr
900 905 910
Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His
915 920 925
Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu
930 935 940
Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu
945 950 955 960
Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly
965 970 975
Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
980 985
<210> 25
<211> 672
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的CHAP11_M23-2638_Ami2638_CBD2638
<400> 25
Met Arg Gly Ser His His His His His His Gly Ser Met Gln Ala Lys
1 5 10 15
Leu Thr Lys Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys
20 25 30
Gln Phe Asn Val Asp Leu Trp Tyr Gly Phe Gln Cys Phe Asp Tyr Ala
35 40 45
Asn Ala Gly Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly
50 55 60
Ala Lys Asp Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val
65 70 75 80
Tyr Gln Asn Thr Pro Asp Phe Leu Ala Gln Pro Gly Asp Met Val Val
85 90 95
Phe Gly Ser Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile
100 105 110
Glu Ala Thr Leu Asp Tyr Ile Ile Val Tyr Glu Gln Asn Trp Leu Gly
115 120 125
Gly Gly Trp Thr Asp Gly Ile Glu Gln Pro Gly Trp Gly Trp Glu Lys
130 135 140
Val Thr Arg Arg Gln His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg
145 150 155 160
Pro Asn Phe Lys Ser Glu Thr Ala Pro Arg Ser Val Gln Ser Pro Thr
165 170 175
Gln Ala Pro Lys Lys Glu Thr Ala Gly Ser Met Leu Thr Ala Ile Asp
180 185 190
Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr
195 200 205
Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly
210 215 220
Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe Asp Val
225 230 235 240
Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly Thr Val
245 250 255
Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val Ile Arg
260 265 270
Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gln Arg Gly Ser
275 280 285
Met Arg Phe Val Val Gly Asp Lys Val Asn Gln Gly Asp Ile Ile Gly
290 295 300
Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val His Leu
305 310 315 320
His Leu Gln Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys Ser Gln
325 330 335
Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn
340 345 350
Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu Lys His
355 360 365
Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro
370 375 380
Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met Thr Pro
385 390 395 400
Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly Thr His
405 410 415
Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val Leu Trp
420 425 430
Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln Trp Ala
435 440 445
Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg
450 455 460
Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu Lys Val
465 470 475 480
Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr
485 490 495
Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His Arg Ser
500 505 510
Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile
515 520 525
Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp
530 535 540
Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp
545 550 555 560
Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala
565 570 575
Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His
580 585 590
Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys
595 600 605
Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln
610 615 620
Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val
625 630 635 640
Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr
645 650 655
Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
660 665 670
<210> 26
<211> 714
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的Ami11_M23-2638_Ami2638_CBD2638
<400> 26
Met Arg Gly Ser His His His His His His Gly Ser Lys Pro Gln Pro
1 5 10 15
Lys Ala Val Glu Leu Lys Ile Ile Lys Asp Val Val Lys Gly Tyr Asp
20 25 30
Leu Pro Lys Arg Gly Ser Asn Pro Lys Gly Ile Val Ile His Asn Asp
35 40 45
Ala Gly Ser Lys Gly Ala Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val
50 55 60
Asn Ala Pro Leu Ser Arg Leu Glu Ala Gly Ile Ala His Ser Tyr Val
65 70 75 80
Ser Gly Asn Thr Val Trp Gln Ala Leu Asp Glu Ser Gln Val Gly Trp
85 90 95
His Thr Ala Asn Gln Ile Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val
100 105 110
Cys Gln Ser Met Gly Ala Asp Asn Ala Thr Phe Leu Lys Asn Glu Gln
115 120 125
Ala Thr Phe Gln Glu Cys Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro
130 135 140
Ala Asn Arg Asn Thr Ile Arg Leu His Asn Glu Phe Thr Ser Thr Ser
145 150 155 160
Cys Pro His Arg Ser Ser Val Leu His Thr Gly Phe Asp Pro Val Thr
165 170 175
Arg Gly Leu Leu Pro Glu Asp Lys Arg Leu Gln Leu Lys Asp Tyr Phe
180 185 190
Ile Lys Gln Ile Arg Ala Tyr Met Asp Gly Lys Ile Pro Val Ala Thr
195 200 205
Val Ser Asn Glu Ser Ser Ala Ser Ser Asn Thr Val Lys Pro Val Ala
210 215 220
Ser Ala Gly Ser Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly
225 230 235 240
Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn
245 250 255
Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe
260 265 270
Cys Gly Gly Tyr His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn
275 280 285
Asp Val Pro Ala Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr
290 295 300
Gly Asn Phe Gly Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp
305 310 315 320
Trp Ile Tyr Gly His Leu Gln Arg Gly Ser Met Arg Phe Val Val Gly
325 330 335
Asp Lys Val Asn Gln Gly Asp Ile Ile Gly Leu Gln Gly Asn Ser Asn
340 345 350
Tyr Tyr Asp Asn Pro Met Ser Val His Leu His Leu Gln Leu Arg Pro
355 360 365
Lys Asp Ala Lys Lys Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala
370 375 380
Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser
385 390 395 400
Lys Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile
405 410 415
Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val
420 425 430
Ile His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp
435 440 445
Leu Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser
450 455 460
Val Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr
465 470 475 480
Val Glu Trp His Cys Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly
485 490 495
Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe
500 505 510
Leu Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys
515 520 525
Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu
530 535 540
Phe Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly
545 550 555 560
Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr
565 570 575
Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val
580 585 590
Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys
595 600 605
Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn
610 615 620
Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr
625 630 635 640
Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His
645 650 655
Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu
660 665 670
Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu
675 680 685
Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly
690 695 700
Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
705 710
<210> 27
<211> 652
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的CHAPTw_Ami2638_M23-LST_CBD2638
<400> 27
Met Arg Gly Ser His His His His His His Gly Ser Met Lys Thr Leu
1 5 10 15
Lys Gln Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn Thr Gly Thr Asp
20 25 30
Phe Asp Gly Leu Tyr Gly Tyr Gln Cys Met Asp Leu Ala Val Asp Tyr
35 40 45
Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp Gly Asn Ala Lys
50 55 60
Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr Val Tyr Lys Asn
65 70 75 80
Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val Val Trp Thr Thr
85 90 95
Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val Thr Asn Pro Asp
100 105 110
Pro Tyr Gly Asp Leu Gln Tyr Val Thr Val Leu Glu Gln Asn Trp Asn
115 120 125
Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile Arg Thr His Asp
130 135 140
Tyr Thr Gly Ile Thr His Phe Ile Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met
210 215 220
Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly
225 230 235 240
Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val
245 250 255
Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln
260 265 270
Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro
275 280 285
Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu
290 295 300
Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg
305 310 315 320
Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His
325 330 335
Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr
340 345 350
Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr
355 360 365
Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln
370 375 380
Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val
385 390 395 400
Lys Ala Thr Asp Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn
405 410 415
Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn
420 425 430
Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro
435 440 445
Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn
450 455 460
Tyr Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His
465 470 475 480
Arg Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp
485 490 495
Tyr Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr
500 505 510
Ser Thr Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser
515 520 525
Asn Ser Thr Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr
530 535 540
Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys
545 550 555 560
Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr
565 570 575
Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr
580 585 590
Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr
595 600 605
Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr
610 615 620
Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys
625 630 635 640
Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
645 650
<210> 28
<211> 650
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的CHAPTw_Ami2638_M23-LST_CWT-LST
<400> 28
Met Arg Gly Ser His His His His His His Gly Ser Met Lys Thr Leu
1 5 10 15
Lys Gln Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn Thr Gly Thr Asp
20 25 30
Phe Asp Gly Leu Tyr Gly Tyr Gln Cys Met Asp Leu Ala Val Asp Tyr
35 40 45
Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp Gly Asn Ala Lys
50 55 60
Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr Val Tyr Lys Asn
65 70 75 80
Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val Val Trp Thr Thr
85 90 95
Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val Thr Asn Pro Asp
100 105 110
Pro Tyr Gly Asp Leu Gln Tyr Val Thr Val Leu Glu Gln Asn Trp Asn
115 120 125
Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile Arg Thr His Asp
130 135 140
Tyr Thr Gly Ile Thr His Phe Ile Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met
210 215 220
Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly
225 230 235 240
Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val
245 250 255
Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln
260 265 270
Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro
275 280 285
Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu
290 295 300
Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg
305 310 315 320
Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His
325 330 335
Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr
340 345 350
Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr
355 360 365
Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln
370 375 380
Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val
385 390 395 400
Lys Ala Thr Asp Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn
405 410 415
Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn
420 425 430
Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro
435 440 445
Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn
450 455 460
Tyr Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His
465 470 475 480
Arg Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp
485 490 495
Tyr Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr
500 505 510
Ser Thr Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser
515 520 525
Asn Ser Thr Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr
530 535 540
Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys
545 550 555 560
Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr
565 570 575
Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met
580 585 590
Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu
595 600 605
Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser
610 615 620
Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn
625 630 635 640
Thr Leu Gly Val Leu Trp Gly Thr Ile Lys
645 650
<210> 29
<211> 517
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的M23-LST_Ami2638_CBD2638
<400> 29
Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His
1 5 10 15
Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr
20 25 30
Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp
35 40 45
Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys
50 55 60
Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile Gly
65 70 75 80
Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr Met His Leu Ser
85 90 95
Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile Ile
100 105 110
Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe
115 120 125
Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro Met
130 135 140
Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr
145 150 155 160
Pro Thr Pro Asn Thr Gly Glu Leu Leu Arg Pro Lys Asp Ala Lys Lys
165 170 175
Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp
180 185 190
Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val
195 200 205
Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile
210 215 220
Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr
225 230 235 240
Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu
245 250 255
Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg
260 265 270
Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys
275 280 285
Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu
290 295 300
Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu
305 310 315 320
Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro
325 330 335
Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser
340 345 350
Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr
355 360 365
Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile
370 375 380
Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr
385 390 395 400
Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys
405 410 415
Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp
420 425 430
Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile
435 440 445
Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val
450 455 460
Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp
465 470 475 480
Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr
485 490 495
Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu
500 505 510
Trp Gly Glu Ile Lys
515
<210> 30
<211> 506
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的LST_LST
<400> 30
Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His
1 5 10 15
Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr
20 25 30
Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp
35 40 45
Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys
50 55 60
Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile Gly
65 70 75 80
Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr Met His Leu Ser
85 90 95
Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile Ile
100 105 110
Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe
115 120 125
Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro Met
130 135 140
Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr
145 150 155 160
Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr
165 170 175
Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg
180 185 190
Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly Val Leu Lys Ala
195 200 205
Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln Asp Gly His Val
210 215 220
Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile Tyr Leu Pro Val
225 230 235 240
Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val Leu Trp Gly Thr
245 250 255
Ile Lys Glu Leu Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn
260 265 270
Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn
275 280 285
Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro
290 295 300
Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn
305 310 315 320
Tyr Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His
325 330 335
Arg Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp
340 345 350
Tyr Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr
355 360 365
Ser Thr Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser
370 375 380
Asn Ser Thr Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr
385 390 395 400
Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys
405 410 415
Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr
420 425 430
Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met
435 440 445
Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu
450 455 460
Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser
465 470 475 480
Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn
485 490 495
Thr Leu Gly Val Leu Trp Gly Thr Ile Lys
500 505
<210> 31
<211> 414
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的M23-LST_M23-LST_CWT-LST
<400> 31
Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His
1 5 10 15
Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr
20 25 30
Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp
35 40 45
Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys
50 55 60
Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile Gly
65 70 75 80
Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr Met His Leu Ser
85 90 95
Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile Ile
100 105 110
Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe
115 120 125
Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro Met
130 135 140
Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr
145 150 155 160
Pro Thr Pro Asn Thr Gly Glu Leu Ala Ala Thr His Glu His Ser Ala
165 170 175
Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro
180 185 190
Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn
195 200 205
Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala
210 215 220
Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn
225 230 235 240
Asp Gly Val His Arg Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val
245 250 255
Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly
260 265 270
Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe Gln Arg Met Val
275 280 285
Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro Met Pro Phe Leu Lys
290 295 300
Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn
305 310 315 320
Thr Gly Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser
325 330 335
Ala Ser Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro
340 345 350
Phe Arg Ser Met Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile
355 360 365
His Tyr Asp Glu Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr
370 375 380
Thr Gly Asn Ser Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn
385 390 395 400
Lys Ser Thr Asn Thr Leu Gly Val Leu Trp Gly Thr Ile Lys
405 410
<210> 32
<211> 628
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的M23-2638_Ami2638_CBD2638_CBD2638
<400> 32
Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala
1 5 10 15
Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg
20 25 30
Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu
35 40 45
Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe
50 55 60
Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly
65 70 75 80
Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val
85 90 95
Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gln Arg
100 105 110
Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gln Gly Asp Ile
115 120 125
Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val
130 135 140
His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys
145 150 155 160
Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn
165 170 175
Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu
180 185 190
Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro
195 200 205
Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser Met
210 215 220
Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly
225 230 235 240
Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val
245 250 255
Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln
260 265 270
Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro
275 280 285
Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu
290 295 300
Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg
305 310 315 320
Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His
325 330 335
Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr
340 345 350
Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr
355 360 365
Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln
370 375 380
Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val
385 390 395 400
Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala
405 410 415
Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg
420 425 430
Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys
435 440 445
Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val
450 455 460
Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val
465 470 475 480
Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu
485 490 495
Ile Lys Glu Leu Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile
500 505 510
Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln
515 520 525
Ile Val Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr
530 535 540
Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile
545 550 555 560
Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu
565 570 575
Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly
580 585 590
His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met
595 600 605
Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp
610 615 620
Gly Glu Ile Lys
625
<210> 33
<211> 741
<212> DNA
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 33
gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60
ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120
attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180
tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240
atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300
ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360
aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420
ggaaaagcag gtggtacagt aactccaacg ccgaatacag gttggaaaac aaacaaatat 480
ggcacactat ataaatcaga gtcagctagc ttcacaccta atacagatat aataacaaga 540
acgactggtc catttagaag catgccgcag tcaggagtct taaaagcagg tcaaacaatt 600
cattatgatg aagtgatgaa acaagacggt catgtttggg taggttatac aggtaacagt 660
ggccaacgta tttacttgcc tgtaagaaca tggaataaat ctactaatac tttaggtgtt 720
ctttggggaa ctataaagtg a 741
<210> 34
<211> 246
<212> PRT
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 34
Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys
1 5 10 15
Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His
20 25 30
Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile
35 40 45
Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly
50 55 60
Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr
65 70 75 80
Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala
85 90 95
Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro
100 105 110
His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala
115 120 125
Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly
130 135 140
Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr
145 150 155 160
Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp
165 170 175
Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly
180 185 190
Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln
195 200 205
Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile
210 215 220
Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val
225 230 235 240
Leu Trp Gly Thr Ile Lys
245
<210> 35
<211> 94
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 35
Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser
1 5 10 15
Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro
20 25 30
Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile
35 40 45
Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp
50 55 60
Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp
65 70 75 80
Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
85 90
<210> 36
<211> 92
<212> PRT
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 36
Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser
1 5 10 15
Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg
20 25 30
Ser Met Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr
35 40 45
Asp Glu Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly
50 55 60
Asn Ser Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser
65 70 75 80
Thr Asn Thr Leu Gly Val Leu Trp Gly Thr Ile Lys
85 90
<210> 37
<211> 146
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 37
Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser
1 5 10 15
Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg
20 25 30
Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr
35 40 45
His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala
50 55 60
Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly
65 70 75 80
Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly
85 90 95
His Leu Gln Arg Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn
100 105 110
Gln Gly Asp Ile Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn
115 120 125
Pro Met Ser Val His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys
130 135 140
Lys Asp
145
<210> 38
<211> 140
<212> PRT
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 38
Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys
1 5 10 15
Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His
20 25 30
Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile
35 40 45
Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly
50 55 60
Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr
65 70 75 80
Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala
85 90 95
Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro
100 105 110
His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala
115 120 125
Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr
130 135 140
<210> 39
<211> 169
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 39
Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val Ile His
1 5 10 15
Asn Asp Tyr Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr
20 25 30
Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr
35 40 45
Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu
50 55 60
Trp His Cys Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu
65 70 75 80
Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu
85 90 95
Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr
100 105 110
Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe
115 120 125
Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly
130 135 140
Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile
145 150 155 160
Lys Arg Ile Lys His Tyr Tyr Asp Gly
165
<210> 40
<211> 163
<212> PRT
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 40
Asn Pro Lys Gly Ile Val Ile His Asn Asp Ala Gly Ser Lys Gly Ala
1 5 10 15
Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val Asn Ala Pro Leu Ser Arg
20 25 30
Leu Glu Ala Gly Ile Ala His Ser Tyr Val Ser Gly Asn Thr Val Trp
35 40 45
Gln Ala Leu Asp Glu Ser Gln Val Gly Trp His Thr Ala Asn Gln Ile
50 55 60
Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val Cys Gln Ser Met Gly Ala
65 70 75 80
Asp Asn Ala Thr Phe Leu Lys Asn Glu Gln Ala Thr Phe Gln Glu Cys
85 90 95
Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro Ala Asn Arg Asn Thr Ile
100 105 110
Arg Leu His Asn Glu Phe Thr Ser Thr Ser Cys Pro His Arg Ser Ser
115 120 125
Val Leu His Thr Gly Phe Asp Pro Val Thr Arg Gly Leu Leu Pro Glu
130 135 140
Asp Lys Arg Leu Gln Leu Lys Asp Tyr Phe Ile Lys Gln Ile Arg Ala
145 150 155 160
Tyr Met Asp
<210> 41
<211> 158
<212> PRT
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 41
Met Ser Ile Ile Met Glu Val Ala Thr Met Gln Ala Lys Leu Thr Lys
1 5 10 15
Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys Gln Phe Asn
20 25 30
Val Asp Leu Trp Tyr Gly Phe Gln Cys Phe Asp Tyr Ala Asn Ala Gly
35 40 45
Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly Ala Lys Asp
50 55 60
Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val Tyr Gln Asn
65 70 75 80
Thr Pro Asp Phe Leu Ala Gln Pro Gly Asp Met Val Val Phe Gly Ser
85 90 95
Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile Glu Ala Thr
100 105 110
Leu Asp Tyr Ile Ile Val Tyr Glu Gln Asn Trp Leu Gly Gly Gly Trp
115 120 125
Thr Asp Gly Ile Glu Gln Pro Gly Trp Gly Trp Glu Lys Val Thr Arg
130 135 140
Arg Gln His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg Pro
145 150 155
<210> 42
<211> 140
<212> PRT
<213> 葡萄球菌噬菌体Twort（Staphylococcus phage Twort）
<400> 42
Met Lys Thr Leu Lys Gln Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn
1 5 10 15
Thr Gly Thr Asp Phe Asp Gly Leu Tyr Gly Tyr Gln Cys Met Asp Leu
20 25 30
Ala Val Asp Tyr Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp
35 40 45
Gly Asn Ala Lys Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr
50 55 60
Val Tyr Lys Asn Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val
65 70 75 80
Val Trp Thr Thr Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val
85 90 95
Thr Asn Pro Asp Pro Tyr Gly Asp Leu Gln Tyr Val Thr Val Leu Glu
100 105 110
Gln Asn Trp Asn Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile
115 120 125
Arg Thr His Asp Tyr Thr Gly Ile Thr His Phe Ile
130 135 140
<210> 43
<211> 12
<212> PRT
<213> 人工（artificial）
<220>
<221> SITE
<223> His-标记
<400> 43
Met Arg Gly Ser His His His His His His Gly Ser
1 5 10
<210> 44
<211> 1497
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的Ply2638
<400> 44
atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60
acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120
tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180
catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240
acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300
aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360
gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420
cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660
tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720
acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780
ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840
tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900
gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960
aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020
ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080
ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200
aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260
ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320
ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380
gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440
cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaataa 1497
<210> 45
<211> 2019
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的CHAP11-M23-2638_Ami2638_CBD2638
<400> 45
atgagaggat cgcatcacca tcaccatcac ggatccatgc aagcaaaatt aactaaaaat 60
gagtttatag agtggttgaa aacttctgag ggaaaacaat tcaatgtgga cttatggtat 120
ggatttcaat gctttgatta tgccaatgct ggttggaaag ttttgtttgg attacttcta 180
aaaggtttag gtgcaaaaga tattccgttc gctaacaact tcgacggatt agctactgta 240
taccaaaata caccggactt cttagcacaa cctggcgaca tggtggtatt cggtagcaac 300
tacggtgctg gatatggtca cgttgcatgg gtaattgaag caactttaga ttacatcatt 360
gtatatgagc agaattggct aggcggtggc tggactgacg gaatcgaaca acccggctgg 420
ggttgggaaa aagttacaag acgacaacat gcttatgatt tccctatgtg gtttatccgt 480
ccgaatttta aaagtgagac agcgccacga tcagttcaat ctcctacaca agcacctaaa 540
aaagaaacag ctggatccat gctaactgct attgactatc ttacgaaaaa aggttggaaa 600
atatcatctg accctcgcac ttacgatggt taccctaaaa actacggcta cagaaattac 660
catgaaaacg gcattaatta tgatgagttt tgtggtggtt atcatagagc ttttgatgtt 720
tacagtaacg aaactaacga cgtgcctgct gttactagcg gaacagttat tgaagcaaac 780
gattacggta attttggtgg tacattcgtt attagagacg ctaacgataa cgattggata 840
tatgggcatc tacaacgtgg ctcaatgcga tttgttgtag gcgacaaagt caatcaaggt 900
gacattattg gtttacaagg taatagcaac tattacgaca atcctatgag tgtacattta 960
catttacaat tacgccctaa agacgcaaag aaagatgaaa aatcacaagt atgtagtggt 1020
ttggctatgg aaaaatatga cattacaaat ttaaatgcta aacaagataa atcaaagaat 1080
gggagcgtga aagagttgaa acatatctat tcaaaccata ttaaaggtaa caagattaca 1140
gcaccaaaac ctagtattca aggtgtggtc atccacaatg attatggtag tatgacacct 1200
agtcaatact taccatggtt atatgcacgt gagaataacg gtacacacgt taacggttgg 1260
gctagtgttt atgcaaatag aaacgaagtg ctttggtatc atccgacaga ctacgtagag 1320
tggcattgtg gtaatcaatg ggcaaatgct aacttaatcg gatttgaagt gtgtgagtcg 1380
tatcctggta gaatctcgga caaattattc ttagaaaatg aagaagcgac attgaaagta 1440
gctgcggatg tgatgaagtc gtacggatta ccagttaatc gcaacactgt acgtctgcat 1500
aacgaattct tcggaacttc ttgtccacat cgttcgtggg acttgcatgt tggcaaaggt 1560
gagccttaca caactactaa tattaataaa atgaaagact acttcatcaa acgcatcaaa 1620
cattattatg acggtggaaa gctagaagta agcaaagcag caactatcaa acaatctgac 1680
gttaagcaag aagttaaaaa gcaagaagca aaacaaattg tgaaagcaac agattggaaa 1740
cagaataaag atggcatttg gtataaagct gaacatgctt cgttcacagt gacagcacca 1800
gagggaatta tcacaagata caaaggtcct tggactggtc acccacaagc tggtgtatta 1860
caaaaaggtc aaacgattaa atatgatgag gttcaaaaat ttgacggtca tgtttgggta 1920
tcgtgggaaa cgtttgaggg cgaaactgta tacatgccgg tacgcacatg ggacgctaaa 1980
actggtaaag ttggtaagtt gtggggcgaa attaaataa 2019
<210> 46
<211> 2145
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的Ami11_M23-2638_Ami2638_CBD2638
<400> 46
atgagaggat cgcatcacca tcaccatcac ggatccaagc cacaacctaa agcagtagaa 60
cttaaaatca tcaaagatgt ggttaaaggt tatgacctac ctaagcgtgg tagtaaccct 120
aaaggtatag ttatacacaa cgacgcaggg agcaaagggg cgactgctga agcatatcgt 180
aacggattag taaatgcacc tttatcaaga ttagaagcgg gcattgcgca tagttacgta 240
tcaggcaaca cagtttggca agccttagat gaatcacaag taggttggca taccgctaat 300
caaataggta ataaatatta ttacggtatt gaagtatgtc aatcaatggg cgcagataac 360
gcgacattct taaaaaatga acaggcaact ttccaagaat gcgctagatt gttgaaaaaa 420
tggggattac cagcaaacag aaatacaatc agattgcaca atgaatttac ttcaacatca 480
tgccctcata gaagttcggt tttacacact ggttttgacc cagtaactcg cggtctattg 540
ccagaagaca agcggttgca acttaaagac tactttatca agcagattag ggcgtacatg 600
gatggtaaaa taccggttgc cactgtctct aatgagtcaa gcgcttcaag taatacagtt 660
aaaccagttg caagtgcagg atccatgcta actgctattg actatcttac gaaaaaaggt 720
tggaaaatat catctgaccc tcgcacttac gatggttacc ctaaaaacta cggctacaga 780
aattaccatg aaaacggcat taattatgat gagttttgtg gtggttatca tagagctttt 840
gatgtttaca gtaacgaaac taacgacgtg cctgctgtta ctagcggaac agttattgaa 900
gcaaacgatt acggtaattt tggtggtaca ttcgttatta gagacgctaa cgataacgat 960
tggatatatg ggcatctaca acgtggctca atgcgatttg ttgtaggcga caaagtcaat 1020
caaggtgaca ttattggttt acaaggtaat agcaactatt acgacaatcc tatgagtgta 1080
catttacatt tacaattacg ccctaaagac gcaaagaaag atgaaaaatc acaagtatgt 1140
agtggtttgg ctatggaaaa atatgacatt acaaatttaa atgctaaaca agataaatca 1200
aagaatggga gcgtgaaaga gttgaaacat atctattcaa accatattaa aggtaacaag 1260
attacagcac caaaacctag tattcaaggt gtggtcatcc acaatgatta tggtagtatg 1320
acacctagtc aatacttacc atggttatat gcacgtgaga ataacggtac acacgttaac 1380
ggttgggcta gtgtttatgc aaatagaaac gaagtgcttt ggtatcatcc gacagactac 1440
gtagagtggc attgtggtaa tcaatgggca aatgctaact taatcggatt tgaagtgtgt 1500
gagtcgtatc ctggtagaat ctcggacaaa ttattcttag aaaatgaaga agcgacattg 1560
aaagtagctg cggatgtgat gaagtcgtac ggattaccag ttaatcgcaa cactgtacgt 1620
ctgcataacg aattcttcgg aacttcttgt ccacatcgtt cgtgggactt gcatgttggc 1680
aaaggtgagc cttacacaac tactaatatt aataaaatga aagactactt catcaaacgc 1740
atcaaacatt attatgacgg tggaaagcta gaagtaagca aagcagcaac tatcaaacaa 1800
tctgacgtta agcaagaagt taaaaagcaa gaagcaaaac aaattgtgaa agcaacagat 1860
tggaaacaga ataaagatgg catttggtat aaagctgaac atgcttcgtt cacagtgaca 1920
gcaccagagg gaattatcac aagatacaaa ggtccttgga ctggtcaccc acaagctggt 1980
gtattacaaa aaggtcaaac gattaaatat gatgaggttc aaaaatttga cggtcatgtt 2040
tgggtatcgt gggaaacgtt tgagggcgaa actgtataca tgccggtacg cacatgggac 2100
gctaaaactg gtaaagttgg taagttgtgg ggcgaaatta aataa 2145
<210> 47
<211> 1959
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的CHAPTw_Ami2638_M23-LST_CBD2638
<400> 47
atgagaggat cgcatcacca tcaccatcac ggatccatga aaaccctgaa acaagcagag 60
tcctacatta agagtaaagt aaatacagga actgattttg atggtttata tgggtatcag 120
tgtatggact tagcagtaga ttatatttac catgtaacag atggtaaaat aagaatgtgg 180
ggtaatgcta aggatgcgat aaataactct tttggtggta ctgctacggt atataaaaac 240
taccctgctt ttagacctaa gtacggtgat gtagtcgtat ggactactgg taattttgca 300
acttatggtc atatcgcaat agttactaac cctgaccctt atggagacct tcaatatgtt 360
acagttcttg aacaaaactg gaacggtaac gggatttata aaaccgagtt agctacaatc 420
agaacacacg attacacagg aattacacat tttattaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660
tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720
acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780
ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840
tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900
gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960
aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020
ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080
ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200
aaagcaacag atgctgcaac acatgaacat tcagcacaat ggttgaataa ttacaaaaaa 1260
ggatatggtt acggtcctta tccattaggt ataaatggcg gtatgcacta cggagttgat 1320
ttttttatga atattggaac accagtaaaa gctatttcaa gcggaaaaat agttgaagct 1380
ggttggagta attacggagg aggtaatcaa ataggtctta ttgaaaatga tggagtgcat 1440
agacaatggt atatgcatct aagtaaatat aatgttaaag taggagatta tgtcaaagct 1500
ggtcaaataa tcggttggtc tggaagcact ggttattcta cagcaccaca tttacacttc 1560
caaagaatgg ttaattcatt ttcaaattca actgcccaag atccaatgcc tttcttaaag 1620
agcgcaggat atggaaaagc aggtggtaca gtaactccaa cgccgaatac aggttggaaa 1680
cagaataaag atggcatttg gtataaagct gaacatgctt cgttcacagt gacagcacca 1740
gagggaatta tcacaagata caaaggtcct tggactggtc acccacaagc tggtgtatta 1800
caaaaaggtc aaacgattaa atatgatgag gttcaaaaat ttgacggtca tgtttgggta 1860
tcgtgggaaa cgtttgaggg cgaaactgta tacatgccgg tacgcacatg ggacgctaaa 1920
actggtaaag ttggtaagtt gtggggcgaa attaaataa 1959
<210> 48
<211> 1554
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的M23-LST_Ami2638_CBD2638
<400> 48
atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60
caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120
ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180
tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240
cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300
aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360
tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420
caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480
ccaacgccga atacaggtga gctcttacgc cctaaagacg caaagaaaga tgaaaaatca 540
caagtatgta gtggtttggc tatggaaaaa tatgacatta caaatttaaa tgctaaacaa 600
gataaatcaa agaatgggag cgtgaaagag ttgaaacata tctattcaaa ccatattaaa 660
ggtaacaaga ttacagcacc aaaacctagt attcaaggtg tggtcatcca caatgattat 720
ggtagtatga cacctagtca atacttacca tggttatatg cacgtgagaa taacggtaca 780
cacgttaacg gttgggctag tgtttatgca aatagaaacg aagtgctttg gtatcatccg 840
acagactacg tagagtggca ttgtggtaat caatgggcaa atgctaactt aatcggattt 900
gaagtgtgtg agtcgtatcc tggtagaatc tcggacaaat tattcttaga aaatgaagaa 960
gcgacattga aagtagctgc ggatgtgatg aagtcgtacg gattaccagt taatcgcaac 1020
actgtacgtc tgcataacga attcttcgga acttcttgtc cacatcgttc gtgggacttg 1080
catgttggca aaggtgagcc ttacacaact actaatatta ataaaatgaa agactacttc 1140
atcaaacgca tcaaacatta ttatgacggt ggaaagctag aagtaagcaa agcagcaact 1200
atcaaacaat ctgacgttaa gcaagaagtt aaaaagcaag aagcaaaaca aattgtgaaa 1260
gcaacagatt ggaaacagaa taaagatggc atttggtata aagctgaaca tgcttcgttc 1320
acagtgacag caccagaggg aattatcaca agatacaaag gtccttggac tggtcaccca 1380
caagctggtg tattacaaaa aggtcaaacg attaaatatg atgaggttca aaaatttgac 1440
ggtcatgttt gggtatcgtg ggaaacgttt gagggcgaaa ctgtatacat gccggtacgc 1500
acatgggacg ctaaaactgg taaagttggt aagttgtggg gcgaaattaa ataa 1554
<210> 49
<211> 1887
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的M23-2638_Ami2638_CBD2638_CBD2638
<400> 49
atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60
acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120
tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180
catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240
acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300
aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360
gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420
cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480
tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540
caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600
aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660
tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720
acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780
ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840
tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900
gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960
aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020
ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080
ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200
aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260
ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320
ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380
gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440
cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaagagctc 1500
ggtggaaagc tagaagtaag caaagcagca actatcaaac aatctgacgt taagcaagaa 1560
gttaaaaagc aagaagcaaa acaaattgtg aaagcaacag attggaaaca gaataaagat 1620
ggcatttggt ataaagctga acatgcttcg ttcacagtga cagcaccaga gggaattatc 1680
acaagataca aaggtccttg gactggtcac ccacaagctg gtgtattaca aaaaggtcaa 1740
acgattaaat atgatgaggt tcaaaaattt gacggtcatg tttgggtatc gtgggaaacg 1800
tttgagggcg aaactgtata catgccggta cgcacatggg acgctaaaac tggtaaagtt 1860
ggtaagttgt ggggcgaaat taaataa 1887
<210> 50
<211> 3461
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> pQE-30载体
<400> 50
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 60
attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 120
ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct 180
gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag 240
aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga 300
atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc 360
actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 420
cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 480
aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 540
ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg 600
gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 660
tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 720
tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 780
tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 840
ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 900
ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt 960
aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 1020
tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg 1080
aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1140
cctgagtagg acaaatccgc cctctagagc tgcctcgcgc gtttcggtga tgacggtgaa 1200
aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 1260
agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 1320
acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 1380
ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1440
accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1500
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1560
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1620
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1680
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1740
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1800
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1860
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1920
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1980
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 2040
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 2100
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 2160
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2220
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2280
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 2340
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 2400
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2460
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 2520
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 2580
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 2640
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 2700
ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 2760
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2820
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 2880
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 2940
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3000
gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3060
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 3120
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 3180
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 3240
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 3300
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 3360
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 3420
cctataaaaa taggcgtatc acgaggccct ttcgtcttca c 3461
<210> 51
<211> 127
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 51
Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val
1 5 10 15
Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr
20 25 30
Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala
35 40 45
Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly
50 55 60
Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr
65 70 75 80
Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser
85 90 95
Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp
100 105 110
Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
115 120 125
<210> 52
<211> 106
<212> PRT
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 52
Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys
1 5 10 15
Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr
20 25 30
Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met
35 40 45
Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu
50 55 60
Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser
65 70 75 80
Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn
85 90 95
Thr Leu Gly Val Leu Trp Gly Thr Ile Lys
100 105
<210> 53
<211> 190
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 53
Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser
1 5 10 15
Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg
20 25 30
Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr
35 40 45
His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala
50 55 60
Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly
65 70 75 80
Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly
85 90 95
His Leu Gln Arg Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn
100 105 110
Gln Gly Asp Ile Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn
115 120 125
Pro Met Ser Val His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys
130 135 140
Lys Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr
145 150 155 160
Asp Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser
165 170 175
Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly
180 185 190
<210> 54
<211> 154
<212> PRT
<213> 模仿葡萄球菌（Staphylococcus simulans）
<400> 54
Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys
1 5 10 15
Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His
20 25 30
Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile
35 40 45
Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly
50 55 60
Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr
65 70 75 80
Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala
85 90 95
Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro
100 105 110
His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala
115 120 125
Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly
130 135 140
Gly Thr Val Thr Pro Thr Pro Asn Thr Gly
145 150
<210> 55
<211> 246
<212> PRT
<213> 金黄色葡萄球菌噬菌体2638a（Staphylococcus aureus bacteriophage2638a）
<400> 55
Glu Lys Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile
1 5 10 15
Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser Val Lys
20 25 30
Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr
35 40 45
Ala Pro Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly
50 55 60
Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn
65 70 75 80
Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn
85 90 95
Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly
100 105 110
Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser
115 120 125
Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala
130 135 140
Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val
145 150 155 160
Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys
165 170 175
Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr
180 185 190
Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys
195 200 205
His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile
210 215 220
Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln
225 230 235 240
Ile Val Lys Ala Thr Asp
245
<210> 56
<211> 220
<212> PRT
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 56
Pro Lys Lys Glu Thr Ala Lys Pro Gln Pro Lys Ala Val Glu Leu Lys
1 5 10 15
Ile Ile Lys Asp Val Val Lys Gly Tyr Asp Leu Pro Lys Arg Gly Ser
20 25 30
Asn Pro Lys Gly Ile Val Ile His Asn Asp Ala Gly Ser Lys Gly Ala
35 40 45
Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val Asn Ala Pro Leu Ser Arg
50 55 60
Leu Glu Ala Gly Ile Ala His Ser Tyr Val Ser Gly Asn Thr Val Trp
65 70 75 80
Gln Ala Leu Asp Glu Ser Gln Val Gly Trp His Thr Ala Asn Gln Ile
85 90 95
Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val Cys Gln Ser Met Gly Ala
100 105 110
Asp Asn Ala Thr Phe Leu Lys Asn Glu Gln Ala Thr Phe Gln Glu Cys
115 120 125
Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro Ala Asn Arg Asn Thr Ile
130 135 140
Arg Leu His Asn Glu Phe Thr Ser Thr Ser Cys Pro His Arg Ser Ser
145 150 155 160
Val Leu His Thr Gly Phe Asp Pro Val Thr Arg Gly Leu Leu Pro Glu
165 170 175
Asp Lys Arg Leu Gln Leu Lys Asp Tyr Phe Ile Lys Gln Ile Arg Ala
180 185 190
Tyr Met Asp Gly Lys Ile Pro Val Ala Thr Val Ser Asn Glu Ser Ser
195 200 205
Ala Ser Ser Asn Thr Val Lys Pro Val Ala Ser Ala
210 215 220
<210> 57
<211> 206
<212> PRT
<213> 金黄色葡萄球菌噬菌体phi 11（Staphylococcus aureus phage phi 11）
<400> 57
Met Ser Ile Ile Met Glu Val Ala Thr Met Gln Ala Lys Leu Thr Lys
1 5 10 15
Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys Gln Phe Asn
20 25 30
Val Asp Leu Trp Tyr Gly Phe Gln Cys Phe Asp Tyr Ala Asn Ala Gly
35 40 45
Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly Ala Lys Asp
50 55 60
Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val Tyr Gln Asn
65 70 75 80
Thr Pro Asp Phe Leu Ala Gln Pro Gly Asp Met Val Val Phe Gly Ser
85 90 95
Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile Glu Ala Thr
100 105 110
Leu Asp Tyr Ile Ile Val Tyr Glu Gln Asn Trp Leu Gly Gly Gly Trp
115 120 125
Thr Asp Gly Ile Glu Gln Pro Gly Trp Gly Trp Glu Lys Val Thr Arg
130 135 140
Arg Gln His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg Pro Asn Phe
145 150 155 160
Lys Ser Glu Thr Ala Pro Arg Ser Val Gln Ser Pro Thr Gln Ala Pro
165 170 175
Lys Lys Glu Thr Ala Lys Pro Gln Pro Lys Ala Val Glu Leu Lys Ile
180 185 190
Ile Lys Asp Val Val Lys Gly Tyr Asp Leu Pro Lys Arg Gly
195 200 205
<210> 58
<211> 187
<212> PRT
<213> 葡萄球菌噬菌体Twort（Staphylococcus phage Twort）
<400> 58
Met Lys Thr Leu Lys Gln Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn
1 5 10 15
Thr Gly Thr Asp Phe Asp Gly Leu Tyr Gly Tyr Gln Cys Met Asp Leu
20 25 30
Ala Val Asp Tyr Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp
35 40 45
Gly Asn Ala Lys Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr
50 55 60
Val Tyr Lys Asn Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val
65 70 75 80
Val Trp Thr Thr Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val
85 90 95
Thr Asn Pro Asp Pro Tyr Gly Asp Leu Gln Tyr Val Thr Val Leu Glu
100 105 110
Gln Asn Trp Asn Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile
115 120 125
Arg Thr His Asp Tyr Thr Gly Ile Thr His Phe Ile Arg Pro Asn Phe
130 135 140
Ala Thr Glu Ser Ser Val Lys Lys Lys Asp Thr Lys Lys Lys Pro Lys
145 150 155 160
Pro Ser Asn Arg Asp Gly Ile Asn Lys Asp Lys Ile Val Tyr Asp Arg
165 170 175
Thr Asn Ile Asn Tyr Asn Met Val Lys Arg Gly
180 185
<210> 59
<211> 4546
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> pHGFP_CBD2638_c载体
<400> 59
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 60
attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 120
ggatcgcatc accatcacca tcacggatcc atgagtaaag gagaagaact tttcactgga 180
gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 240
ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 300
ggaaaactac ctgttccatg gccaacactt gtcactactt tcgcgtatgg tcttcaatgc 360
tttgcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa 420
ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 480
gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 540
aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 600
tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 660
attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 720
ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 780
cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 840
catggcatgg atgaactata caaagagctc ggtggaaagc tagaagtaag caaagcagca 900
actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 960
aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1020
ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1080
ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1140
gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1200
cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaataagtc 1260
gacctgcagc caagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac 1320
ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 1380
tgagaatcca agctagcttg gcgagatttt caggagctaa ggaagctaaa atggagaaaa 1440
aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 1500
catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 1560
ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 1620
cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga 1680
tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 1740
cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 1800
tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 1860
tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 1920
acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 1980
tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 2040
tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 2100
gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata 2160
aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2220
gctctcctga gtaggacaaa tccgccctct agagctgcct cgcgcgtttc ggtgatgacg 2280
gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 2340
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 2400
ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 2460
gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 2520
aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 2580
tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2640
aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2700
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2760
tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2820
ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2880
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2940
ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3000
ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3060
gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3120
agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 3180
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3240
aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3300
aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3360
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3420
aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 3480
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 3540
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 3600
cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3660
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3720
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3780
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3840
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3900
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3960
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 4020
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 4080
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 4140
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4200
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 4260
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 4320
acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 4380
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 4440
tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 4500
attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac 4546
<210> 60
<211> 1143
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的GFP_CBD2638
<400> 60
atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60
actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120
gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180
actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240
caatgctttg cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg 300
cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360
cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420
gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480
aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540
cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600
ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660
aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720
attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140
taa 1143
<210> 61
<211> 1539
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的GFP_CBD2638_CBD2638 var.1
<400> 61
atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60
actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120
gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180
actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240
caatgctttg cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg 300
cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360
cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420
gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480
aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540
cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600
ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660
aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720
attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140
gagctcggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200
caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260
aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320
attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380
ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440
gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500
aaagttggta agttgtgggg cgaaattaaa taagtcgac 1539
<210> 62
<211> 1560
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> Hist-标记的GFP_CBD2638_CBD2638 var. 2
<400> 62
atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60
actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120
gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180
actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240
caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 300
cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360
cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420
gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480
aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540
cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600
ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660
aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720
attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140
ggtaccggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200
caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260
aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320
attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380
ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440
gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500
aaagttggta agttgtgggg cgaaattaaa gtcgacctgc agccaagctt aattagctga 1560
<210> 63
<211> 1944
<212> DNA
<213> 人工（artificial）
<220>
<221> CDS
<223> His-标记的GFP_CBD2638_CBD2638_CBD2638
<400> 63
atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60
actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120
gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180
actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240
caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 300
cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360
cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420
gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480
aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540
cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600
ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660
aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720
attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140
ggtaccggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200
caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260
aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320
attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380
ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440
gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500
aaagttggta agttgtgggg cgaaattaaa gtcgacggtg gaaagctaga agtaagcaaa 1560
gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 1620
attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 1680
gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1740
ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1800
aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1860
ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1920
ctgcagccaa gcttaattag ctga 1944
<210> 64
<211> 380
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的GFP_CBD2638
<400> 64
Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly
1 5 10 15
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly
20 25 30
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp
35 40 45
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys
50 55 60
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu
65 70 75 80
Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe
85 90 95
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
100 105 110
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly
115 120 125
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu
130 135 140
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
145 150 155 160
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn
165 170 175
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp
180 185 190
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro
195 200 205
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
210 215 220
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
225 230 235 240
Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu
245 250 255
Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu
260 265 270
Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys
275 280 285
Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr
290 295 300
Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr
305 310 315 320
Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr
325 330 335
Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr
340 345 350
Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys
355 360 365
Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
370 375 380
<210> 65
<211> 510
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的GFP_CBD2638_CBD2638 var.1
<400> 65
Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly
1 5 10 15
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly
20 25 30
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp
35 40 45
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys
50 55 60
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu
65 70 75 80
Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe
85 90 95
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
100 105 110
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly
115 120 125
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu
130 135 140
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
145 150 155 160
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn
165 170 175
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp
180 185 190
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro
195 200 205
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
210 215 220
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
225 230 235 240
Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu
245 250 255
Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu
260 265 270
Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys
275 280 285
Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr
290 295 300
Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr
305 310 315 320
Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr
325 330 335
Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr
340 345 350
Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys
355 360 365
Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Glu Leu Gly Gly
370 375 380
Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys
385 390 395 400
Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp
405 410 415
Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser
420 425 430
Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro
435 440 445
Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile
450 455 460
Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp
465 470 475 480
Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp
485 490 495
Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
500 505 510
<210> 66
<211> 519
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的GFP_CBD2638_CBD2638 var. 2
<400> 66
Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly
1 5 10 15
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly
20 25 30
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp
35 40 45
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys
50 55 60
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu
65 70 75 80
Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe
85 90 95
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
100 105 110
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly
115 120 125
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu
130 135 140
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
145 150 155 160
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn
165 170 175
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp
180 185 190
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro
195 200 205
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
210 215 220
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
225 230 235 240
Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu
245 250 255
Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu
260 265 270
Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys
275 280 285
Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr
290 295 300
Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr
305 310 315 320
Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr
325 330 335
Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr
340 345 350
Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys
355 360 365
Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Gly Thr Gly Gly
370 375 380
Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys
385 390 395 400
Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp
405 410 415
Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser
420 425 430
Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro
435 440 445
Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile
450 455 460
Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp
465 470 475 480
Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp
485 490 495
Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Val Asp
500 505 510
Leu Gln Pro Ser Leu Ile Ser
515
<210> 67
<211> 647
<212> PRT
<213> 人工（artificial）
<220>
<221> CHAIN
<223> His-标记的GFP_CBD2638_CBD2638_CBD2638
<400> 67
Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly
1 5 10 15
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly
20 25 30
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp
35 40 45
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys
50 55 60
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu
65 70 75 80
Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe
85 90 95
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
100 105 110
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly
115 120 125
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu
130 135 140
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
145 150 155 160
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn
165 170 175
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp
180 185 190
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro
195 200 205
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
210 215 220
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
225 230 235 240
Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu
245 250 255
Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln Glu
260 265 270
Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp Lys
275 280 285
Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr
290 295 300
Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr
305 310 315 320
Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr
325 330 335
Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr
340 345 350
Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys
355 360 365
Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Gly Thr Gly Gly
370 375 380
Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys
385 390 395 400
Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp
405 410 415
Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser
420 425 430
Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro
435 440 445
Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile
450 455 460
Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp
465 470 475 480
Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp
485 490 495
Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Val Asp
500 505 510
Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp
515 520 525
Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala
530 535 540
Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His
545 550 555 560
Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys
565 570 575
Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln
580 585 590
Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val
595 600 605
Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr
610 615 620
Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys
625 630 635 640
Leu Gln Pro Ser Leu Ile Ser
645

Claims (10)

1.一种包含第一核苷酸序列的核酸分子，其中，所述核苷酸序列与SEQ ID NO:12具有至少80％的序列同一性。
2.根据权利要求1所述的核酸分子，其中，所述第一核苷酸序列编码细胞壁结合域，所述细胞壁结合域结合葡萄球菌属的肽聚糖细胞壁。
3.根据权利要求1或2所述的核酸分子，其中，所述第一核苷酸序列源于金黄色葡萄球菌噬菌体Φ2638a内溶素。
4.根据权利要求1-3中任一项所述的核酸分子，其中，所述核酸分子与SEQ ID NO:1具有至少80％的序列同一性，或者其中所述核酸分子进一步包含编码裂解域的异源核苷酸序列。
5.根据权利要求1-3中任一项所述的核酸分子，其中，所述核酸分子进一步包含编码裂解域的异源核苷酸序列，其中所述裂解域是第二核苷酸序列和第三核苷酸序列，以及其中所述第二核苷酸序列编码M23内肽酶域并且所述第三核苷酸序列编码酰胺酶域。
6.根据权利要求5所述的核酸分子，其中，所述第二核苷酸序列和第三核苷酸序列源于编码酶的基团，所述酶选自由金黄色葡萄球菌噬菌体Φ2638a内溶素、金黄色葡萄球菌噬菌体Φ11内溶素、金黄色葡萄球菌噬菌体ΦTwort内溶素、和模仿葡萄球菌溶葡萄球菌素组成的组。
7.根据权利要求6所述的核酸分子，其中，所述第二核苷酸序列与SEQ ID:14或15具有至少80％的序列同一性以及所述第三核苷酸序列与SEQ ID NO:16或17具有至少80％的序列同一性。
8.根据权利要求7所述的核酸分子，其中，所述核苷酸分子与SEQ ID NO:9具有至少80％的序列同一性。
9.根据权利要求4-8中任一项所述的核酸分子，进一步包含编码CHAP(半胱氨酸、组氨酸依赖性酰胺水解酶/肽酶)域的第四核苷酸序列。
10.一种多肽，由如在权利要求1-9中任一项所识别的核酸分子编码。
CN201711457491.2A 2011-05-04 2011-05-04 一种多肽 Pending CN108192902A (zh)

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