JP6261086B2 - ポリペプチド - Google Patents
ポリペプチド Download PDFInfo
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- JP6261086B2 JP6261086B2 JP2014509259A JP2014509259A JP6261086B2 JP 6261086 B2 JP6261086 B2 JP 6261086B2 JP 2014509259 A JP2014509259 A JP 2014509259A JP 2014509259 A JP2014509259 A JP 2014509259A JP 6261086 B2 JP6261086 B2 JP 6261086B2
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Description
第1の態様において、第1のヌクレオチド配列を含む、又は第1のヌクレオチド配列からなる核酸分子であって、前記ヌクレオチド配列が、配列番号12(本明細書で用いられる全ての配列番号の大要については、表1を参照されたい)と80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99又は100%の配列同一性を有する、核酸分子を提供する。好ましくは、前記第1のヌクレオチド配列は、少なくとも282、285、290、300、310、320、330、340、350、360又は370個のヌクレオチド、より好ましくは、少なくとも381個のヌクレオチドの長さ、及び/又は最大で510、480、450、420又は390個のヌクレオチドの長さを有する。好ましくは、前記核酸分子は、少なくとも282、285、290、300、310、320、330、340、350、360、370個のヌクレオチド、より好ましくは少なくとも381個のヌクレオチドの長さ、及び/又は最大で4500、4200、3900、及び/又は3600個のヌクレオチドの長さを有する。好ましくは、前記核酸分子は、少なくとも1200、1230、1260、1290、1320、1350、1380、1410、1440、1470、1500、1530又は1560個のヌクレオチドの長さ、及び/又は最大で4500、4200、3900、及び/又は3600個のヌクレオチドの長さを有する。また、少なくとも1890、1920、1950、1980、2010、2040、2070、2100、2130又は2160個のヌクレオチドの長さ、及び/又は最大で4500、4200、3900、及び/又は3600個のヌクレオチドの長さを有する本発明に係る核酸分子も好ましい。前記第1のヌクレオチド配列は、スタフィロコッカス属のペプチドグリカン細胞壁と結合する細胞壁結合ドメインをコードするのが好ましい。前記第1のヌクレオチド配列は、S.アウレウスバクテリオファージΦ2638aエンドリシンに由来するのが好ましい。
ポリペプチド
核酸構築物
細胞
方法
i)前の章で定義された核酸構築物を含む細胞中で、前記ポリペプチドを生産するステップ、任意選択で
ii)前記ポリペプチドを精製するステップ、及び任意選択で
iii)前記精製したポリペプチドを凍結乾燥するステップ
を含む。
方法
i)前記ポリペプチドを、キレート化合物を含む緩衝液で透析するステップ、
ii)前記ポリペプチドを、二価金属イオンを含有する緩衝液で透析するステップであって、好ましくは前記二価金属イオンが、Mn2+、Co2+、Cu2+、及びZn2+からなる群より選択されるステップ
を含む。
組成物
使用
方法
定義
配列同一性
核酸構築物、形質転換、発現ベクター、作動可能に連結した、発現、制御配列、ポリペプチド
構築物
菌株、培養条件、ファージ、及びプラスミド
6x−Hisでタグ付けした(配列番号43)融合タンパク質の過剰発現のために、E.コリXL1BlueMRF’、及びE.コリSureを使用した。両方の株を、LB−PE培地中で、30℃で、プラスミド選択のための100μg/mlアンピシリン及び30μg/mlテトラサイクリンと共に培養した。Ply2638A遺伝子又はそのドメインコード領域を増幅させるためのテンプレートとして、ファージ2638A溶解産物を用いた。ファージTwort溶解産物からCHAPTwドメイン(配列番号19)を増幅した。ファージ11由来のシステイン−ヒスチジン依存性アミダーゼ/ペプチダーゼ(CHAP)ドメイン、及びアミダーゼドメイン(配列番号18及び17、それぞれ;Donovanら、2006及び2008;Navarreら、1999;Sass及びBierbaum 2007)を、Donovan,D.M.氏から進呈されたphi11自己溶解酵素遺伝子を含有するpet21aベクターから増幅した。M23−LST(配列番号15)及びCWT−LST(配列番号13)ドメインを増幅するためのテンプレートとして成熟リソスタフィンの配列(配列番号33)を含有するプラスミドLT1215を使用した。
DNA技術及びクローニングの手順
組換え融合タンパク質の発現及び精製
組換えタンパク質の凍結乾燥
細胞壁結合分析
定量的な蛍光分析
溶解分析
二価金属イオンのPly2638の活性への影響
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTAA
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGGAGCTCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
TGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
TGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGAT
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATAT
GGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGT
AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCA
ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCT
ATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATT
ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGELLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIKELAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGELAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAACTATAAAGTGA
AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
WKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
WKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
MLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKD
AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGY
NKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDG
NPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMD
MSIIMEVATMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRP
MKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFI
MRGSHHHHHHGS
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCAAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAACAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATGTAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCGCATGCGAGCTCGGTACCCCGGGTCGACCTGCAGCCAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC
GKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
GKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK
MLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKG
AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTG
EKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATD
PKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASA
MSIIMEVATMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRG
MKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFATYGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIRPNFATESSVKKKDTKKKPKPSNRDGINKDKIVYDRTNINYNMVKRG
CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAAGTCGACCTGCAGCCAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAAGTCGAC
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGGTACCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGTCGACCTGCAGCCAAGCTTAATTAGCTGA
ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGGTACCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAAGTCGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAACTGCAGCCAAGCTTAATTAGCTGA
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDLQPSLIS
MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKLQPSLIS
Claims (26)
- 第1のヌクレオチド配列を含む核酸分子であって、前記ヌクレオチド配列が、配列番号12と少なくとも85%の配列同一性を有し、
前記第1のヌクレオチド配列が、スタフィロコッカス(Staphylococcus)属のペプチドグリカン細胞壁と結合する細胞壁結合ドメインをコードし、
前記核酸分子が、溶解ドメインをコードする異種ヌクレオチド配列をさらに含み、前記溶解ドメインが、第2及び第3のヌクレオチド配列であり、前記第2のヌクレオチド配列が、配列番号15と少なくとも85%の配列同一性を有し、かつ、M23エンドペプチダーゼドメインをコードし、前記第3のヌクレオチド配列が、配列番号16と少なくとも85%の配列同一性を有し、かつ、アミダーゼドメインをコードし、前記核酸分子が、配列番号9と少なくとも85%の配列同一性を有する、
核酸分子。 - 前記第1のヌクレオチド配列が、S.アウレウス(S.aureus)バクテリオファージΦ2638aエンドリシンに由来する、請求項1に記載の核酸分子。
- 前記第2のヌクレオチド配列が、S.シミュランス(S.simulans)のリソスタフィンをコードする遺伝子に由来し、前記第3のヌクレオチド配列がS.アウレウスバクテリオファージΦ2638aエンドリシンをコードする遺伝子に由来する、請求項1に記載の核酸分子。
- CHAP(システイン、ヒスチジン依存性アミドヒドロラーゼ/ペプチダーゼ)ドメインをコードする第4のヌクレオチド配列をさらに含む、請求項1〜3のいずれか一項に記載の核酸分子。
- 前記第4のヌクレオチド配列が、S.アウレウスバクテリオファージΦ11又はS.アウレウスバクテリオファージΦTwortエンドリシンに由来する、請求項4に記載の核酸分子。
- 前記第4のヌクレオチド配列が、配列番号18又は配列番号19と少なくとも85%の配列同一性を有する、請求項5に記載の核酸分子。
- 配列番号1によってコードされたS.アウレウスバクテリオファージΦ2638aエンドリシンと比較して、同じ若しくは増加した溶解活性、又は同じ若しくは低い最適pHを有するポリペプチドをコードする、請求項1〜6のいずれか一項に記載の核酸分子。
- (1)請求項1〜7のいずれか一項に特定された第1のヌクレオチド配列によってコードされる、スタフィロコッカス属のペプチドグリカン細胞壁と結合する細胞壁結合ドメイン、
(2)請求項1〜7のいずれか一項に特定された第2のヌクレオチド配列によってコードされる、M23エンドペプチダーゼドメイン、及び
(3)請求項1〜7のいずれか一項に特定された第3のヌクレオチド配列によってコードされる、アミダーゼドメインを
含む、ポリペプチド。 - 請求項1〜7のいずれか一項に記載の核酸分子を含む核酸構築物。
- 細胞、対象、又は無細胞発現系においてコードされたポリペプチドの産生又は発現を指示する1つ又は複数の制御配列に作動可能に連結した、請求項9に記載の核酸構築物を含む発現ベクター。
- 請求項9に記載の核酸構築物又は請求項10に記載の発現ベクターを含む細胞であって、前記細胞が、微生物細胞、原核細胞又は真核細胞である、細胞。
- 請求項8に記載のポリペプチドを生産する方法であって、前記ポリペプチドを、請求項9に記載の核酸構築物を含む請求項11に記載の細胞中で生産するステップを含む、方法。
- 前記ポリペプチドを精製するステップをさらに含む、請求項12に記載の方法。
- 前記精製したポリペプチドを凍結乾燥するステップをさらに含む、請求項13に記載の方法。
- 請求項8に記載のポリペプチド又は請求項12〜14のいずれか一項に記載の方法によって入手できるポリペプチドを処理することにより、強化された溶解活性を有するポリペプチドを生産する方法であって、
i)前記ポリペプチドを、キレート化合物を含む緩衝液で透析するステップ、
ii)前記ポリペプチドを、二価金属イオンを含有する緩衝液で透析するステップである、ステップ
を含む、方法。 - 前記二価金属イオンが、Co2+、Cu2+、Mn2+、及びZn2+からなる群より選択される、請求項15に記載の方法。
- 請求項1〜7のいずれか一項に記載の核酸分子、請求項8に記載のポリペプチド、又は請求項12〜14のいずれか一項に記載の方法によって入手できるポリペプチド、又は請求項9に記載の核酸構築物、又は請求項10に記載のベクター、又は請求項11に記載の細胞を含む組成物。
- 1種又は複数の追加の活性成分を含む、請求項17に記載の組成物。
- 薬剤として使用するための、請求項17又は18に記載の組成物。
- 感染性疾患の治療における薬剤として使用するための、請求項19に記載の組成物。
- 抗菌剤としての、請求項1〜7のいずれか一項に記載の核酸分子、請求項8に記載のポリペプチド、又は請求項12〜14のいずれか一項に記載の方法によって入手できるポリペプチド、請求項9に記載の核酸構築物、請求項10に記載のベクター、請求項11に記載の細胞、又は請求項17若しくは18に記載の組成物の使用。
- スタフィロコッカスを検出するための、
請求項1〜7のいずれか一項に記載の核酸分子、
請求項8に記載のポリペプチド、若しくは請求項12〜14のいずれか一項に記載の方法によって入手できるポリペプチド、又は
請求項9に記載の核酸構築物、又は
請求項10に記載のベクター、又は
請求項11に記載の細胞、又は
請求項17若しくは18に記載の組成物
の使用。 - 請求項17又は18に記載の組成物を動物(ヒトを除く)対象に投与することによる、感染性疾患の治療、遅延、又は予防方法。
- 前記活性成分がバクテリオファージ及び抗生物質からなる群より選択される、請求項18に記載の組成物。
- 食品添加剤又は消毒剤としての請求項21に記載の使用。
- 診断薬を製造するための、
請求項1〜7のいずれか一項に記載の核酸分子、
請求項8に記載のポリペプチド、若しくは請求項12〜14のいずれか一項に記載の方法によって入手できるポリペプチド、又は
請求項9に記載の核酸構築物、又は
請求項10に記載のベクター、又は
請求項11に記載の細胞、又は
請求項17若しくは18に記載の組成物
の使用。
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PCT/NL2011/050307 WO2012150858A1 (en) | 2011-05-04 | 2011-05-04 | A polypeptide |
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DK3019185T3 (da) | 2013-07-11 | 2021-02-15 | Micreos Human Health Bv | Kombinationsbehandling for atopisk dermatitis |
WO2015175774A1 (en) | 2014-05-14 | 2015-11-19 | Trustees Of Dartmouth College | Deimmunized lysostaphin and methods of use |
AU2016231141B2 (en) | 2015-03-12 | 2022-03-03 | Micreos Human Health B.V. | Combination of bactericidal agent with a lysosomotropic alkalinising agent for the treatment of a bacterial infection |
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PL3085777T3 (pl) | 2020-05-18 |
EP2705144A1 (en) | 2014-03-12 |
BR112013028251A2 (pt) | 2017-01-17 |
PL2705144T3 (pl) | 2016-12-30 |
EP2705144B2 (en) | 2019-08-28 |
KR101911114B1 (ko) | 2018-10-23 |
IL229247B (en) | 2019-03-31 |
JP2014519815A (ja) | 2014-08-21 |
DK3085777T3 (da) | 2020-02-03 |
US10174300B2 (en) | 2019-01-08 |
ES2589784T3 (es) | 2016-11-16 |
US9382298B2 (en) | 2016-07-05 |
EP3085777A1 (en) | 2016-10-26 |
HK1195791A1 (zh) | 2014-11-21 |
CA2834924A1 (en) | 2012-11-08 |
CN108192902A (zh) | 2018-06-22 |
EP3085777B1 (en) | 2019-11-06 |
DK2705144T4 (da) | 2019-10-21 |
ES2768777T3 (es) | 2020-06-23 |
KR20140066976A (ko) | 2014-06-03 |
WO2012150858A1 (en) | 2012-11-08 |
AU2011367262A1 (en) | 2013-11-21 |
CN103703127A (zh) | 2014-04-02 |
ES2589784T5 (es) | 2020-04-21 |
EP2705144B1 (en) | 2016-06-08 |
US20160369255A1 (en) | 2016-12-22 |
AU2011367262B2 (en) | 2016-11-03 |
SG194776A1 (en) | 2013-12-30 |
CA2834924C (en) | 2022-08-16 |
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