CN108158030A - A kind of compound Chinese medicine extract extracting method and its application in cigarette - Google Patents
A kind of compound Chinese medicine extract extracting method and its application in cigarette Download PDFInfo
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
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- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
Abstract
The invention discloses a kind of compound Chinese medicine extract extracting method, step includes：1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, be immersed in ethanol water and extract, soak and immersion slag are filtered to obtain after extraction；2) the aqueous solution high temperature and pressure extraction that slag immerses malic acid, citric acid is impregnated, filtering acquisition extracting solution and extraction slag after extraction；3) slag and water mixing defibrination are extracted, slurries complex enzyme zymohydrolysis adds in L leucines after enzymolysis and carries out Maillard reaction, acquisition reaction solution after the completion of reaction；4) soak and extracting solution are concentrated under reduced pressure respectively, and reaction solution is concentrated under reduced pressure into paste, then liquid after concentration and condensed cream are sufficiently mixed, that is, obtain compound Chinese medicine extract.The present invention significantly reduces the content of HCN and benzo [a] pyrene in main flume, reduces cigarette smoke harmfulness, harm of the smoking to human body is reduced while consumer demand is met by adding compound Chinese medicine extract into cigarette shreds.
Description
Technical field
The invention belongs to cigarette additive technical field more particularly to a kind of compound Chinese medicine extract extracting method and its
Application in cigarette.
Background technology
Chinese herbal medicine is applied reduces cigarette smoke in cigarette mainly has the harm of human body two aspects to act on, on the one hand,
Chinese herbal medicine extract adds in the tobacco, and by the pipe tobacco that burns, the Chinese herbal medicine substance in pipe tobacco is by gradient-heated, active ingredient
Feedback, gasification, volatilization and distillation are steamed, and enters the harmful components in cigarette smoke in capture flue gas or blocks harmful components
Generation；On the other hand, adding advantageous to body medicinal herb components in cigarette can enter with flue gas in vivo, such as alkaloid, Huang
Ketone, abundant glycosides, plant stay the organic compounds such as alcohol, amino acid, vitamin, Yi Jitie, zinc, selenium and other trace elements.China possesses rich
Rich Chinese herbal medicine resource and theory of traditional Chinese medical science basis, Chinese herbal medicine of the hoof choosing with harmful components in selectivity reducing cigarette fume, and
For mitigating the road that bitting midges are development less harmful Chinese-style cigarette characteristics.
Main flume (MS), be when smoking flue gas by being directly entered the part of respiratory tract after filtering；During suction gap,
Air velocity in cigarette substantially reduces, and nearby oxygen content is abundant for combustion cone, and oxidation reaction mainly occurs, generates side-stream smoke
(SS), directly by cigarette paper dispersal events to environment.Main flume enters lung by the respiratory tract of smoker, can stimulate
Human central nervous system generates excitation, directly determines cigarette smoke organoleptic quality.
In cigarette mainstream flue gas largely in the substance being detrimental to health, such as grain phase nicotine, tar, BaP,
Crotonaldehyde, hydrogen cyanide etc., carbon monoxide, carbon dioxide in gas phase etc..Wherein, nicotine also known as nicotine（Nicotine）, change
Formula is C10H14N2, is high volatility, bitter, the alkaloid with excitation.Nicotine can stimulate central nervous system emerging
It puts forth energy, increases adrenaline secretion, have the function of to refresh oneself and recover from fatigue, increase pleasant sensation, when smoking passes through the quilts such as oral cavity, nasal cavity, tracheae
Absorption of human body.After smoker generates dependence to nicotine, that is, have " smoking addiction ", the long-term nicotine that sucks can reduce human activity
And memory, easily cause angiocardiopathy.Hydrogen cyanide, molecular formula HCN are the extremely toxic substances for seriously endangering health.It is
The reaction product of nitrate, amino acid etc. in tobacco leaf is existed simultaneously in main flume gas phase and grain phase part, and ratio is close to 1：1,
Content is not high, but it is the compound that ciliary toxicity is most strong in cigarette mainstream flue gas.HCN can inhibit in human tissue cell 42 kinds
The activity of respiratory enzyme prevents histocyte from normally using the oxygen in blood, damaging human central nervous system, can in liver
Rapid metabolization becomes rhodanate.The organic matter fragment that tobacco burn incompletely under conditions of high temperature obtains was polymerizeing
Different types of condensed-nuclei aromatics can be formed by being formed in journey（PAH）.What is identified in flue gas has the PAH of carcinogenicity there are about 30 kinds,
Wherein benzo [a] pyrene is carcinogenicity object the most typical.
Increasingly pay attention to today of own bodies health, control and reduction harmful constituents in cigarette flue gas release in people
Amount reduces cigarette smoke harmfulness, and smoking is reduced while consumer demand is met and is of great significance to the harm of human body.
Invention content
To achieve the above object, the present invention provides a kind of compound Chinese medicine extract extracting method, step includes：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, after grinding
Powder be immersed in the mixed solution of second alcohol and water, soaking temperature is 80~90 DEG C, and soaking time >=5h is solid after the completion of impregnating
Liquid separation obtains soak and impregnates slag；
2) mixed liquor is formed in the aqueous solution for impregnating slag immersion malic acid, citric acid, mixed liquor is put into closed container
High pressure extraction is carried out, the extraction pressure is 5~10 standard atmospheric pressures, and balance envelope after the completion is extracted in Extracting temperature >=120 DEG C
Close container inner and outer air pressure, mixed liquor cooled to room temperature, then separation of solid and liquid obtain extracting solution and extraction slag；
3) the extraction slag and water are mixed, is put into defibrination in fiberizer, obtain suspension slurry, the suspension slurry participates in multiple
Enzymolysis liquid is closed, is digested under ultrasonic environment, the solid phase particles after the completion of enzymolysis in filtering removal slurries, then be added dropwise into slurries
A small amount of ammonium hydroxide adjusts pH value, then adds in L-Leu and carries out Maillard reaction and be sufficiently stirred, is reacted after the completion of reaction
Liquid；
4) soak and extracting solution are concentrated under reduced pressure respectively, and the reaction solution is concentrated under reduced pressure into paste, then by soak and
The condensed cream of liquid and reaction solution is sufficiently mixed after extracting solution is concentrated under reduced pressure, that is, obtains the compound Chinese medicine extract.
Further, in the step 1), the Longtube Ground Ivy Herb leaf, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and Pueraria lobota
Root is counted in parts by weight is respectively：10~20 parts of Longtube Ground Ivy Herb leaf, 8~15 parts of cymbopogon distans, 9~16 parts of paris polyphylla leaf, corter pseudolaricis 2~7
2~3 parts of part, 2~3 parts of Caulis Spatholobi and pueraria lobata.
Further, in the step 1), the Longtube Ground Ivy Herb leaf, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and Pueraria lobota
Root is counted in parts by weight is respectively：13 parts of Longtube Ground Ivy Herb leaf, 11 parts of cymbopogon distans, 10 parts of paris polyphylla leaf, 4.5 parts of corter pseudolaricis, Caulis Spatholobi 2
2 parts of part and pueraria lobata.
Further, in the step 1), in the mixed solution of second alcohol and water, the volume ratio of second alcohol and water is 3:7, it grinds
Powder and the solid-liquid mass ratio of ethanol water afterwards is 1:3~5, soak is concentrated under reduced pressure into the 1/5 of original volume in step 4)
~1/4.
Further, in the step 2), malic acid, citric acid aqueous solution in, malic acid, citric acid quality percentage
Content is respectively：20%~25%, 6%~8%, it is 1 to impregnate slag and malic acid, the aqueous solution solid-liquid mass ratio of citric acid:3~5, it is high
Extraction time >=2h is pressed, extracting solution is concentrated under reduced pressure into the 1/5~1/4 of original volume in step 4).
Further, in the step 3), it is 1 to extract slag and water mixing quality ratio:2~3, grinding clearance for 0.05~
0.1mm, containing cellulase, pectase and protease in complex enzyme hydrolysis liquid, cellulase, pectin described in multiplex-enzyme extraction liquid
Enzyme, protease mass percentage be respectively 0.6%~0.8%, 0.05%~0.1%, 0.1%~0.2%, the suspension slurry and
The mass ratio of multiplex-enzyme extraction liquid is 1:0.1~0.3, hydrolysis temperature is 45 ~ 50 DEG C, and enzymolysis time is more than 2h, and the L- is bright
The additive amount of propylhomoserin is 0.03~0.1 times of suspension slurry quality, and the Maillard reaction temperature is 85 ~ 100 DEG C, the reaction time
For 1 ~ 5h, pH value is 8 ~ 9.
The invention also discloses compound Chinese medicine extract obtained above is added to cigarette shreds as cigarette additive
In application.
Further, the additive amount of the compound Chinese medicine extract is the 0.2 ~ 0.3% of pipe tobacco weight.
As can be seen from the above technical solutions, it is an advantage of the invention that：The present invention is compound by being added into cigarette shreds
Chinese medical extract, the Chinese herbal medicine substance in pipe tobacco by gradient-heated, steamed feedback, gasification, volatilization and distillation, gone forward side by side by active ingredient
Enter the harmful components captured in cigarette smoke in flue gas, significantly reduce the content of HCN and benzo [a] pyrene in main flume, drop
Low cigarette smoke harmfulness reduces harm of the smoking to human body while consumer demand is met, and valency is used with good
Value.
Specific embodiment
It is described in detail with reference to embodiment：
Embodiment 1
A kind of compound Chinese medicine extract extracting method, step include：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, the company
Money blade of grass, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：10 parts of Longtube Ground Ivy Herb leaf, rue
2 parts of 8 parts of vanilla, 9 parts of paris polyphylla leaf, 2 parts of corter pseudolaricis, 2 parts of Caulis Spatholobi and pueraria lobata.Powder after grinding is immersed in second alcohol and water
Volume ratio is 3:In 7 ethanol water, it is 1 to impregnate solid-liquid mass ratio:3, soaking temperature is 80~90 DEG C, soaking time 5h,
Separation of solid and liquid obtains soak and impregnates slag after the completion of immersion；
2) it is described impregnate slag and immerse in the aqueous solution of malic acid, citric acid forms mixed liquor, malic acid, citric acid aqueous solution
In, malic acid, citric acid mass percentage be respectively：20%th, 6%, solid-to-liquid ratio is 1 in mixed liquor:3.Mixed liquor is put into
High pressure extraction is carried out in closed container, the pressure of extracting is 8 standard atmospheric pressure, 120 DEG C, extraction time 2h of Extracting temperature,
Extraction after the completion of balance closed container inner and outer air pressure, mixed liquor cooled to room temperature, then separation of solid and liquid obtain extracting solution and
Extract slag；
3) water of 2 times of the extraction slag and its quality is mixed, is put into defibrination in fiberizer, grinding clearance for 0.05~
0.1mm, obtains suspension slurry, and the suspension slurry participates in complex enzyme hydrolysis liquid, digested under ultrasonic environment, wherein complex enzyme hydrolysis
Containing cellulase, pectase and protease in liquid, the matter of cellulase, pectase, protease described in multiplex-enzyme extraction liquid
It is respectively 0.6%, 0.05%, 0.1% to measure percentage composition.The mass ratio of suspension slurry and multiplex-enzyme extraction liquid is 1:0.1, enzymolysis temperature
It is 45 ~ 50 DEG C to spend, enzymolysis time 3h.Solid phase particles after the completion of enzymolysis in filtering removal slurries, then dropwise addition is few into slurries
It measures ammonium hydroxide and adjusts pH value to 8, the L-Leu for then adding in 0.03 times of suspension slurry quality carries out Maillard reaction and fully stirs
It mixes, Maillard reaction temperature is 85 ~ 100 DEG C, reaction time 1h, and reaction solution is obtained after the completion of reaction；
4) soak is concentrated under reduced pressure into the 1/5 of original volume, and extracting solution is concentrated under reduced pressure into the 1/5 of original volume, the reaction solution
Paste is concentrated under reduced pressure into, the condensed cream of liquid and reaction solution is sufficiently mixed after then soak and extracting solution are concentrated under reduced pressure, that is, is obtained
Obtain the compound Chinese medicine extract.
Embodiment 2
A kind of compound Chinese medicine extract extracting method, step include：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, the company
Money blade of grass, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：13 parts of Longtube Ground Ivy Herb leaf, rue
2 parts of 11 parts of vanilla, 10 parts of paris polyphylla leaf, 4.5 parts of corter pseudolaricis, 2 parts of Caulis Spatholobi and pueraria lobata.Powder after grinding be immersed in ethyl alcohol and
The volume ratio of water is 3:In 7 ethanol water, it is 1 to impregnate solid-liquid mass ratio:3, soaking temperature is 80~90 DEG C, soaking time
5h, separation of solid and liquid obtains soak and impregnates slag after the completion of immersion；
2) it is described impregnate slag and immerse in the aqueous solution of malic acid, citric acid forms mixed liquor, malic acid, citric acid aqueous solution
In, malic acid, citric acid mass percentage be respectively：22%th, 7%, solid-liquid mass ratio is 1 in mixed liquor:3.By mixed liquor
It is put into closed container and carries out high pressure extraction, the pressure of extracting is 5 standard atmospheric pressure, 120 DEG C of Extracting temperature, extraction time
2h is extracted and is balanced closed container inner and outer air pressure after the completion, mixed liquor cooled to room temperature, and then separation of solid and liquid obtains extracting solution
With extraction slag；
3) water of 2 times of the extraction slag and its quality is mixed, is put into defibrination in fiberizer, grinding clearance for 0.05~
0.1mm, obtains suspension slurry, and the suspension slurry participates in complex enzyme hydrolysis liquid, digested under ultrasonic environment, wherein complex enzyme hydrolysis
Containing cellulase, pectase and protease in liquid, the matter of cellulase, pectase, protease described in multiplex-enzyme extraction liquid
It is respectively 0.7%, 0.07%, 0.15% to measure percentage composition.The mass ratio of suspension slurry and multiplex-enzyme extraction liquid is 1:0.2, enzymolysis temperature
It is 45 ~ 50 DEG C to spend, enzymolysis time 2h.Solid phase particles after the completion of enzymolysis in filtering removal slurries, then dropwise addition is few into slurries
It measures ammonium hydroxide and adjusts pH value to 8, the L-Leu for then adding in 0.05 times of suspension slurry quality carries out Maillard reaction and fully stirs
It mixes, Maillard reaction temperature is 85 ~ 100 DEG C, reaction time 2h, and reaction solution is obtained after the completion of reaction；
4) soak is concentrated under reduced pressure into the 1/5 of original volume, and extracting solution is concentrated under reduced pressure into the 1/5 of original volume, the reaction solution
Paste is concentrated under reduced pressure into, the condensed cream of liquid and reaction solution is sufficiently mixed after then soak and extracting solution are concentrated under reduced pressure, that is, is obtained
Obtain the compound Chinese medicine extract.
Embodiment 3
A kind of compound Chinese medicine extract extracting method, step include：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, the company
Money blade of grass, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：15 parts of Longtube Ground Ivy Herb leaf, rue
3 parts of 14 parts of vanilla, 12 parts of paris polyphylla leaf, 5.7 parts of corter pseudolaricis, 3 parts of Caulis Spatholobi and pueraria lobata.Powder after grinding be immersed in ethyl alcohol and
The volume ratio of water is 3:In 7 ethanol water, it is 1 to impregnate solid-liquid mass ratio:4, soaking temperature is 80~90 DEG C, soaking time
5h, separation of solid and liquid obtains soak and impregnates slag after the completion of immersion；
2) it is described impregnate slag and immerse in the aqueous solution of malic acid, citric acid forms mixed liquor, malic acid, citric acid aqueous solution
In, malic acid, citric acid mass percentage be respectively：24%th, 7%, solid-liquid mass ratio is 1 in mixed liquor:5.By mixed liquor
It is put into closed container and carries out high pressure extraction, the pressure of extracting is 9 standard atmospheric pressure, 120 DEG C of Extracting temperature, extraction time
2h is extracted and is balanced closed container inner and outer air pressure after the completion, mixed liquor cooled to room temperature, and then separation of solid and liquid obtains extracting solution
With extraction slag；
3) water of 3 times of the extraction slag and its quality is mixed, is put into defibrination in fiberizer, grinding clearance for 0.05~
0.1mm, obtains suspension slurry, and the suspension slurry participates in complex enzyme hydrolysis liquid, digested under ultrasonic environment, wherein complex enzyme hydrolysis
Containing cellulase, pectase and protease in liquid, the matter of cellulase, pectase, protease described in multiplex-enzyme extraction liquid
It is respectively 0.8%, 0.08%, 0.17% to measure percentage composition.The mass ratio of suspension slurry and multiplex-enzyme extraction liquid is 1:0.3, enzymolysis temperature
It is 45 ~ 50 DEG C to spend, enzymolysis time 2h.Solid phase particles after the completion of enzymolysis in filtering removal slurries, then dropwise addition is few into slurries
It measures ammonium hydroxide and adjusts pH value to 9, the L-Leu for then adding in 0.1 times of suspension slurry quality carries out Maillard reaction and fully stirs
It mixes, Maillard reaction temperature is 85 ~ 100 DEG C, reaction time 3h, and reaction solution is obtained after the completion of reaction；
4) soak is concentrated under reduced pressure into the 1/4 of original volume, and extracting solution is concentrated under reduced pressure into the 1/5 of original volume, the reaction solution
Paste is concentrated under reduced pressure into, the condensed cream of liquid and reaction solution is sufficiently mixed after then soak and extracting solution are concentrated under reduced pressure, that is, is obtained
Obtain the compound Chinese medicine extract.
Embodiment 4
A kind of compound Chinese medicine extract extracting method, step include：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, the company
Money blade of grass, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：18 parts of Longtube Ground Ivy Herb leaf, rue
3 parts of 15 parts of vanilla, 14 parts of paris polyphylla leaf, 6.3 parts of corter pseudolaricis, 3 parts of Caulis Spatholobi and pueraria lobata.Powder after grinding be immersed in ethyl alcohol and
The volume ratio of water is 3:In 7 ethanol water, it is 1 to impregnate solid-liquid mass ratio:5, soaking temperature is 80~90 DEG C, soaking time
5h, separation of solid and liquid obtains soak and impregnates slag after the completion of immersion；
2) it is described impregnate slag and immerse in the aqueous solution of malic acid, citric acid forms mixed liquor, malic acid, citric acid aqueous solution
In, malic acid, citric acid mass percentage be respectively：22%th, 8%, solid-liquid mass ratio is 1 in mixed liquor:5.By mixed liquor
It is put into closed container and carries out high pressure extraction, the pressure of extracting is 9 standard atmospheric pressure, 120 DEG C of Extracting temperature, extraction time
2h is extracted and is balanced closed container inner and outer air pressure after the completion, mixed liquor cooled to room temperature, and then separation of solid and liquid obtains extracting solution
With extraction slag；
3) water of 3 times of the extraction slag and its quality is mixed, is put into defibrination in fiberizer, grinding clearance for 0.05~
0.1mm, obtains suspension slurry, and the suspension slurry participates in complex enzyme hydrolysis liquid, digested under ultrasonic environment, wherein complex enzyme hydrolysis
Containing cellulase, pectase and protease in liquid, the matter of cellulase, pectase, protease described in multiplex-enzyme extraction liquid
It is respectively 0.8%, 0.1%, 0.2% to measure percentage composition.The mass ratio of suspension slurry and multiplex-enzyme extraction liquid is 1:0.3, hydrolysis temperature
It is 45 ~ 50 DEG C, enzymolysis time 2h.Solid phase particles after the completion of enzymolysis in filtering removal slurries, then dropwise addition is a small amount of into slurries
Ammonium hydroxide adjusting pH value to 9, the then L-Leu of 0.08 times of addition suspension slurry quality carry out Maillard reaction and are sufficiently stirred,
Maillard reaction temperature is 85 ~ 100 DEG C, reaction time 4h, and reaction solution is obtained after the completion of reaction；
4) soak is concentrated under reduced pressure into the 1/4 of original volume, and extracting solution is concentrated under reduced pressure into the 1/4 of original volume, the reaction solution
Paste is concentrated under reduced pressure into, the condensed cream of liquid and reaction solution is sufficiently mixed after then soak and extracting solution are concentrated under reduced pressure, that is, is obtained
Obtain the compound Chinese medicine extract.
Embodiment 5
A kind of compound Chinese medicine extract extracting method, step include：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, the company
Money blade of grass, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：20 parts of Longtube Ground Ivy Herb leaf, rue
2 parts of 15 parts of vanilla, 16 parts of paris polyphylla leaf, 7 parts of corter pseudolaricis, 2 parts of Caulis Spatholobi and pueraria lobata.Powder after grinding is immersed in second alcohol and water
Volume ratio be 3:In 7 ethanol water, it is 1 to impregnate solid-liquid mass ratio:5, soaking temperature is 80~90 DEG C, soaking time
5h, separation of solid and liquid obtains soak and impregnates slag after the completion of immersion；
2) it is described impregnate slag and immerse in the aqueous solution of malic acid, citric acid forms mixed liquor, malic acid, citric acid aqueous solution
In, malic acid, citric acid mass percentage be respectively：25%th, 8%, solid-liquid mass ratio is 1 in mixed liquor:5.By mixed liquor
It is put into closed container and carries out high pressure extraction, the extraction pressure is 10 standard atmospheric pressures, 120 DEG C of Extracting temperature, during extraction
Between 2h, extraction after the completion of balance closed container inner and outer air pressure, mixed liquor cooled to room temperature, then separation of solid and liquid extracted
Liquid and extraction slag；
3) water of 3 times of the extraction slag and its quality is mixed, is put into defibrination in fiberizer, grinding clearance for 0.05~
0.1mm, obtains suspension slurry, and the suspension slurry participates in complex enzyme hydrolysis liquid, digested under ultrasonic environment, wherein complex enzyme hydrolysis
Containing cellulase, pectase and protease in liquid, the matter of cellulase, pectase, protease described in multiplex-enzyme extraction liquid
It is respectively 0.6%, 0.1%, 0.2% to measure percentage composition.The mass ratio of suspension slurry and multiplex-enzyme extraction liquid is 1:0.3, hydrolysis temperature
It is 45 ~ 50 DEG C, enzymolysis time 2h.Solid phase particles after the completion of enzymolysis in filtering removal slurries, then dropwise addition is a small amount of into slurries
Ammonium hydroxide adjusting pH value to 8, the then L-Leu of 0.1 times of addition suspension slurry quality carry out Maillard reaction and are sufficiently stirred,
Maillard reaction temperature is 85 ~ 100 DEG C, reaction time 5h, and reaction solution is obtained after the completion of reaction；
4) soak is concentrated under reduced pressure into the 1/5 of original volume, and extracting solution is concentrated under reduced pressure into the 1/5 of original volume, the reaction solution
Paste is concentrated under reduced pressure into, the condensed cream of liquid and reaction solution is sufficiently mixed after then soak and extracting solution are concentrated under reduced pressure, that is, is obtained
Obtain the compound Chinese medicine extract.
Comparative example 1
A kind of compound Chinese medicine extract extracting method, method and step and embodiment 2 are identical, differ only in, in step 2)
Slag is impregnated to immerse in the distilled water for being free of malic acid, citric acid.Other steps are identical with embodiment 2 with parameter, finally obtain
Obtain the compound Chinese medicine extract of this comparative example.
Embodiment 6
Compound Chinese medicine extract made from above-described embodiment 1~5 and comparative example 1 is respectively added to cigarette as cigarette additive
In pipe tobacco, it is finally made cigarette finished product.The additive amount of compound Chinese medicine extract is the 0.2% of pipe tobacco weight.Embodiment will be contained
1~5 and comparative example 1 made from each group cigarette finished product of compound Chinese medicine extract test is lighted in test equipment respectively, simultaneously
The cigarette made of the pipe tobacco for being commonly free of any additive lights test as blank control group in test equipment, receives respectively
Collect the smog after the filtering of mouth stick and be detected comparison, detect the content of benzo [a] pyrene in main flume, while by national cigarette
Measure continuous flow method of the careless industry about hydrogen cyanide in cigarette mainstream flue gas (HCN)（YC/T253-2008）Operation, uses 1mol/
LNaOH solution collects hydrogen cyanide (HCN) burst size in main flume, with the cyanogen of isonicotinic acid -- barbituric acid method detection collection liquid
Change hydrogen（HCN）Content, and with the hydrogen cyanide in the blank control group main flume of unused additive（HCN）Content carries out pair
According to calculating the percentage reduced relative to blank control group hydrogen cyanide content.The results are shown in Table 1.
Table 1
Test group hydrogen cyanide (HCN) content declines percentage benzo [a] pyrene content (mg/50 branch)
Blank control group/6.39
Embodiment 1 43.82% 0.093
Embodiment 2 47.51% 0.084
Embodiment 3 42.19% 0.117
Embodiment 4 40.03% 0.105
Embodiment 5 42.20% 0.108
Comparative example 1 13.17% 3.446
As shown in Table 1, by adding compound Chinese medicine extract into cigarette shreds, HCN and benzene in main flume are significantly reduced
And the content of [a] pyrene, cigarette smoke harmfulness is reduced, harm of the smoking to human body is reduced while consumer demand is met,
With good use value.Comparative example 2 and comparative example 1 are it is found that using malic acid, citric acid to medium-height grass of the present invention
Medicine carries out high pressure extraction, will can be conducive to remove in Longtube Ground Ivy Herb leaf, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata
The beneficiating ingredient of HCN and benzo [a] pyrene extracts so that the harm reduction effect of final extract more optimizes.
Technical solution provided by the present invention is described in detail above, for those of ordinary skill in the art,
The thought of embodiment according to the present invention, there will be changes in specific embodiments and applications, in conclusion this theory
Bright book content should not be construed as limiting the invention.
Claims (8)
1. a kind of compound Chinese medicine extract extracting method, which is characterized in that extraction step includes：
1) Longtube Ground Ivy Herb leaf, cymbopogon distans aerial part, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are mixed and ground, after grinding
Powder be immersed in the mixed solution of second alcohol and water, soaking temperature is 80~90 DEG C, and soaking time >=5h is solid after the completion of impregnating
Liquid separation obtains soak and impregnates slag；
2) mixed liquor is formed in the aqueous solution for impregnating slag immersion malic acid, citric acid, mixed liquor is put into closed container
High pressure extraction is carried out, the extraction pressure is 5~10 standard atmospheric pressures, and balance envelope after the completion is extracted in Extracting temperature >=120 DEG C
Close container inner and outer air pressure, mixed liquor cooled to room temperature, then separation of solid and liquid obtain extracting solution and extraction slag；
3) the extraction slag and water are mixed, is put into defibrination in fiberizer, obtain suspension slurry, the suspension slurry participates in multiple
Enzymolysis liquid is closed, is digested under ultrasonic environment, the solid phase particles after the completion of enzymolysis in filtering removal slurries, then be added dropwise into slurries
A small amount of ammonium hydroxide adjusts pH value, then adds in L-Leu and carries out Maillard reaction and be sufficiently stirred, is reacted after the completion of reaction
Liquid；
4) soak and extracting solution are concentrated under reduced pressure respectively, and the reaction solution is concentrated under reduced pressure into paste, then by soak and
The condensed cream of liquid and reaction solution is sufficiently mixed after extracting solution is concentrated under reduced pressure, that is, obtains the compound Chinese medicine extract.
2. a kind of compound Chinese medicine extract extracting method according to claim 1, which is characterized in that in the step 1),
The Longtube Ground Ivy Herb leaf, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：Longtube Ground Ivy Herb leaf 10
2~3 parts of~20 parts, 8~15 parts of cymbopogon distans, 9~16 parts of paris polyphylla leaf, 2~7 parts of corter pseudolaricis, 2~3 parts of Caulis Spatholobi and pueraria lobata.
3. a kind of compound Chinese medicine extract extracting method according to claim 1, which is characterized in that in the step 1),
The Longtube Ground Ivy Herb leaf, cymbopogon distans, paris polyphylla leaf, corter pseudolaricis, Caulis Spatholobi and pueraria lobata are counted in parts by weight is respectively：Longtube Ground Ivy Herb leaf 13
2 parts of part, 11 parts of cymbopogon distans, 10 parts of paris polyphylla leaf, 4.5 parts of corter pseudolaricis, 2 parts of Caulis Spatholobi and pueraria lobata.
4. according to a kind of compound Chinese medicine extract extracting method of claims 1 to 3 any one of them, which is characterized in that described
In step 1), in the mixed solution of second alcohol and water, the volume ratio of second alcohol and water is 3:7, powder and ethanol water after grinding
Solid-liquid mass ratio be 1:3~5, soak is concentrated under reduced pressure into the 1/5~1/4 of original volume in step 4).
5. according to a kind of compound Chinese medicine extract extracting method of claims 1 to 3 any one of them, which is characterized in that described
In step 2), malic acid, citric acid aqueous solution in, malic acid, citric acid mass percentage be respectively：20%~25%,
6%~8%, it is 1 to impregnate slag and malic acid, the aqueous solution solid-liquid mass ratio of citric acid:3~5, the high pressure extraction time >=2h, step
4) extracting solution is concentrated under reduced pressure into the 1/5~1/4 of original volume in.
6. according to a kind of compound Chinese medicine extract extracting method of claims 1 to 3 any one of them, which is characterized in that described
In step 3), it is 1 to extract slag and water mixing quality ratio:2~3, grinding clearance is 0.05~0.1mm, is contained in complex enzyme hydrolysis liquid
Cellulase, pectase and protease, cellulase described in multiplex-enzyme extraction liquid, pectase, protease quality percentage contain
Amount is respectively 0.6%~0.8%, 0.05%~0.1%, 0.1%~0.2%, the mass ratio of the suspension slurry and multiplex-enzyme extraction liquid
It is 1:0.1~0.3, hydrolysis temperature is 45 ~ 50 DEG C, and enzymolysis time is more than 2h, and the additive amount of the L-Leu is starched to suspend
0.03~0.1 times of liquid quality, the Maillard reaction temperature are 85 ~ 100 DEG C, and the reaction time is 1 ~ 5h, and pH value is 8 ~ 9.
7. application of the compound Chinese medicine extract that the extracting method as described in any one of claim 1~6 is extracted in cigarette, special
Sign is that the compound Chinese medicine extract is added to as cigarette additive in cigarette shreds.
8. application of a kind of compound Chinese medicine extract according to claim 7 in cigarette, which is characterized in that described compound
The additive amount of Chinese medical extract is the 0.2 ~ 0.3% of pipe tobacco weight.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109758785A (en) * | 2019-03-28 | 2019-05-17 | 江西中烟工业有限责任公司 | A kind of preparation method of cigar ultra high pressure extraction liquid Maillard reaction flavor and the application in cigarette |
| CN110702891A (en) * | 2019-11-18 | 2020-01-17 | 江西中烟工业有限责任公司 | Method for testing harm reduction effect of natural herbal extract on long-term smoking harm |
| CN111418885A (en) * | 2020-05-20 | 2020-07-17 | 云南如茨生物科技有限公司 | Preparation method of special spice for heating cigarette products |
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| CN1491596A (en) * | 2003-08-22 | 2004-04-28 | 忠 陈 | Chinese herbal medicine additive in cigarette and its preparing mehtod and use |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758785A (en) * | 2019-03-28 | 2019-05-17 | 江西中烟工业有限责任公司 | A kind of preparation method of cigar ultra high pressure extraction liquid Maillard reaction flavor and the application in cigarette |
| CN110702891A (en) * | 2019-11-18 | 2020-01-17 | 江西中烟工业有限责任公司 | Method for testing harm reduction effect of natural herbal extract on long-term smoking harm |
| CN111418885A (en) * | 2020-05-20 | 2020-07-17 | 云南如茨生物科技有限公司 | Preparation method of special spice for heating cigarette products |
| CN111418885B (en) * | 2020-05-20 | 2021-12-21 | 云南如茨生物科技有限公司 | Heating cigarette product |
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