CN108142424A - The bactericidal composition of a kind of fluorine thiazole pyrrole ethyl ketone and fludioxonil and preparation and its application - Google Patents
The bactericidal composition of a kind of fluorine thiazole pyrrole ethyl ketone and fludioxonil and preparation and its application Download PDFInfo
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- CN108142424A CN108142424A CN201810141552.2A CN201810141552A CN108142424A CN 108142424 A CN108142424 A CN 108142424A CN 201810141552 A CN201810141552 A CN 201810141552A CN 108142424 A CN108142424 A CN 108142424A
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- China
- Prior art keywords
- ethyl ketone
- fludioxonil
- pyrrole ethyl
- thiazole pyrrole
- fluorine thiazole
- Prior art date
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- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 229960002900 methylcellulose Drugs 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 229950007856 mofetil Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229940068984 polyvinyl alcohol Drugs 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- PYODKQIVQIVELM-UHFFFAOYSA-M sodium;2,3-bis(2-methylpropyl)naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S([O-])(=O)=O)=C(CC(C)C)C(CC(C)C)=CC2=C1 PYODKQIVQIVELM-UHFFFAOYSA-M 0.000 description 1
- HIEHAIZHJZLEPQ-UHFFFAOYSA-M sodium;naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 HIEHAIZHJZLEPQ-UHFFFAOYSA-M 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the bactericidal compositions of a kind of fluorine thiazole pyrrole ethyl ketone and fludioxonil and preparation and its application.The combination sterilization composition is made of fluorine thiazole pyrrole ethyl ketone and fludioxonil；Wherein, the mass ratio of the fluorine thiazole pyrrole ethyl ketone and fludioxonil is 1：(0.2~8).Compound sterilizing agent formulation quick-acting and lasting effect of the present invention are good, mechanism of action is unique, action site is more, vegetables concurrent oomycetes disease, gray mold and early blight can be effectively prevented, dead caused by root rot caused by can also effectively preventing oomycetes, Fusariumsp, rhizoctonia, Phomopsis and Phytophthora capsici, sickle-like bacteria；Preparation process is simple, at low cost, remarkable in economical benefits, and diseases prevention spectrum is wide, and biological safety is high.
Description
Technical field
The invention belongs to pesticide field more particularly to the bactericidal compositions and preparation of a kind of fluorine thiazole pyrrole ethyl ketone and fludioxonil
And its application.
Background technology
Usually concurrent multiple diseases, such as concurrent cucumber downy mildew, gray mold of cucumber and cucumber root on cucumber on vegetables
Maize ear rot, concurrent late blight, early blight and gray mold on tomato, concurrent capsicum epidemic disease, Botrytis cinerea and capsicum on capsicum
Dead, the concurrent late blight of potato and target on potato.
Vegetables oomycetes disease is caused by Mastigomycotina oomycetes is infected, wherein being attached most importance to phytophthora, downy mildew, the rotten oomycetes such as mould
Pathogen, the vegetable crops such as main harm Solanaceae, Curcurbitaceae and potato are wanted, causes samping off, epidemic disease, downy mildew, root rot
And dead etc., the substantially underproduction is caused even to have no harvest.Especially downy mildew and phytophthora short incubation period, then infect often, it is planting
Germ, which can rapidly develop, in one season of growth of object causes plant disease epidemic, leads to the heavy losses of agricultural production, such as cucumber cream
Mildew can cause cucumber plant all dead in several days.
Gray mold is a kind of global important aeroborne disease, and flower, fruit, leaf and the stem of crop can fall ill, national each
Ground generally occurs, and in rising trend.Botrytis cinerea (Botrytis cinerea) causes the gray mold of most crops,
Host range is extensive, can infect a variety of fruits and vegetables such as tomato, eggplant, cucumber, capsicum, strawberry, grape, garlic, romaine lettuce, citrus and make
Object.Early blight is to endanger the Major Diseases of potato and tomato, is mainly caused by Solanum rod method Alternaria solani.
In greenhouse or greenhouse, when the weather for encountering continuous several days low temperature and less sunshines, cucumber downy mildew often with cucumber grey mold
Sick concurrent, tomato late blight is often concurrent with graw mold of tomato, in capsicum stalk and fruit epidemic disease often with Botrytis cinerea simultaneously
Hair, the late blight of potato is often concurrent with early blight, and the mixture for carrying out spraying application at present does not have mostly just for a kind of disease
Have while for the medicament of oomycetes disease and gray mold, if mixing use using several medicament barrels, although oomycetes disease simultaneous can be controlled
Evil, gray mold and early blight, but be difficult accurately to grasp dosage, cause overdose or drug effect poor.
Cucumber root rot is often caused by a variety of pathogen Combined Infections, and such as fusarium (Fusarium solani) intends stem
Point mould (Phomopsis sp), corruption mould (Pythium myriotylum) and phytophthora (Phytophthora drechsleri) etc.,
Using 30% hymexazo AP, 800 times of liquid, 3% Evil, 650 times of liquid irrigating roots of mould first frost AP.In addition, fusarium root rot and plan
SP250 times of liquid of 12.5% carbendazim, 60% WP700 times of liquid of good fortune first sulphur, 50% chlorine bromine isocyanide urine also can be used in Phoma sp root rot
Sour SP1000 times of liquid irrigating root.25% metalaxyl WP, 600 times of liquid, 50% fluorine also can be used in rotten mould root rot and phytophthora root rot
The pouring roots such as WP600 times of liquid of second aluminium.Every plant of dosage 200mL or so, fills 1~2 time again when necessary during pouring root, and control effect is often very
Difference urgently develops new and effective medicament in production, improve its control effect to cucumber root rot.
Dead of capsicum can be by Phytophthora capsici (Phytophthora capsici) and Fusarium Solani (erosion arteries and veins fusarium
Fusarium vasinfectum, scouring rush's fusarium Fusarium eguiseti, Fusorium moniliforme Sheldon F.moniliforme, sharp fusarium
F.oxysporum it) individually infects or Combined Infection causes.30% hymexazo AP, 800 times of liquid, the mould first frost AP of 3% Evil can be used
650 times of liquid irrigating roots.In addition, caused by fusarium root rot dead SP250 times of liquid of 12.5% carbendazim, 60% good fortune first also can be used
SP1000 times of 700 times of liquid of sulphur WP, 50% chlorobromide isocyanuric acid liquid irrigating root.Dead also can be used 25% first white caused by Phytophthora capsici
The pouring roots such as clever WP600 times of liquid, 50% fluorine mofetil aluminium 600 times of liquid of WP.Every plant of dosage 200mL or so, fills again when necessary during pouring root
1~2 time, control effect is often very poor, and new and effective medicament is urgently developed in production, and improving it, the prevention of dead is imitated to capsicum
Fruit.
It is the maximally efficient means of controlling plant diseases using chemical agent, but the application of long-term continuous high dose single is killed
Microbial inoculum, the problems such as be easy to causeing residual, environmental pollution and the development of drug resistance of medicament.Rationally there is compounding fungicide expansion to kill
Bacterium spectrum improves control effect, extends dispenser interval, reduce dosage, delay the generation of pathogen drug resistance and development etc. special
Point.Bactericide compounded is one of effective ways to solve the above problems.Current composite bactericide can not realize cucumber downy mildew,
The vegetables such as tomato late blight, eliminating vegetable botrytis, the late blight of potato, target and cucumber root rot and capsicum epidemic disease
The prevention of upper concurrent important disease.
Invention content
The present situation of hair is mixed for the multiple diseases such as downy mildew, late blight, gray mold on vegetables, lacks efficient spray in production
Mist is simultaneous to control oomycetes disease (downy mildew, late blight, epidemic disease) and gray mold, lack the prevention of efficient pouring root by pythium spp, phytophthora,
Fusarium is independent or Combined Infection caused by root rot or the composite bactericide of dead, fungicidal spectrum existing for existing composite bactericide
In place of the deficiencies of relatively narrow, the present invention provides the bactericidal composition of a kind of fluorine thiazole pyrrole ethyl ketone and fludioxonil and preparation and its answer
With, the biocide preparation it is novel, it is efficient, safely, have a wide range of application, vegetable downy mildew, late blight, epidemic disease, gray mold, Huang can be prevented
The diseases such as melon root rot and dead of capsicum.
A kind of combination sterilization composition provided by the invention, it is made of fluorine thiazole pyrrole ethyl ketone and fludioxonil；Wherein, it is described
The mass ratio of fluorine thiazole pyrrole ethyl ketone and the fludioxonil is 1：(0.2~8), such as 1：(0.2~7), 1：(0.2~6), 1：(0.25
~8) or 1：(0.25~7).
Preferably, the mass ratio of the fluorine thiazole pyrrole ethyl ketone and the fludioxonil is 1：(0.25~6), such as 1：(0.25~
5)1：(0.3~6), 1：(0.5~6), 1：(1~6), 1：(0.3~5) or 1：(0.5~5)
It is highly preferred that the mass ratio of the fluorine thiazole pyrrole ethyl ketone and the fludioxonil is 1：(1~5), such as 1：(1~4), 1：
(1~3), 1：(2~5), 1：(2~4), 1：(3~5) or 1：(3~4).
Most preferably, the mass ratio of the fluorine thiazole pyrrole ethyl ketone and the fludioxonil is 1：3.
In a specific embodiment of the present invention, the mass ratio of the fluorine thiazole pyrrole ethyl ketone and the fludioxonil can be 5：1、4：
1、3：1、2：1、1：1、1：2、1：3、1：4、1：5、1：6、1：7 or 1：8.
Invention further provides a kind of compound sterilizing agent formulations, it is combined by compound sterilizing described in any one of the above embodiments
Acceptable auxiliary agent composition on object and pesticidal preparations.
In above-mentioned compound sterilizing agent formulation, the mass percentage of the combination sterilization composition can be 10%~
50%, concretely 20%.
In above-mentioned compound sterilizing agent formulation, the fungicide preparation can be the pesticidal preparations of any one dosage form, including
But it is not limited at least one of following dosage forms：Wettable powder (WP), water dispersible granules (WG), aqueous emulsion (AE), suspending agent
(SC).The dosage form can be used method well-known in the art and prepare, and typically be sufficiently mixed active material and auxiliary agent
Dissolving or grinding are granulated, and preparation use is made.
In above-mentioned compound sterilizing agent formulation, the auxiliary agent includes dispersant, wetting agent, binding agent, thickener, anti-bubble
Agent, disintegrant, filler etc. and other be beneficial to active material in the formulation stablize and play drug effect substance, have no special limit
Fixed, concrete component and dosage are determined by testing.
The dosage form is wettable powder；The auxiliary agent can be it is following a) or b)：A) auxiliary agent is wetting agent and filler；
B) auxiliary agent is dispersant, wetting agent and filler.
In terms of parts by weight, the wettable powder can be made of following components；
Any one of the wettable powder concretely following A 1)-A7)：
1) in terms of parts by weight, the wettable powder is made of following components；
2) in terms of parts by weight, the wettable powder is made of (WP1) following components；
3) in terms of parts by weight, the wettable powder is made of (WP2) following components；
4) in terms of parts by weight, the wettable powder is made of (WP3) following components；
5) in terms of parts by weight, the wettable powder is made of (WP4) following components；
6) in terms of parts by weight, the wettable powder is made of (WP5) following components；
7) in terms of parts by weight, the wettable powder is made of (WP6) following components；
Preparation method of the preparation method of the wettable powder for conventional wettable powder, specifically may include walking as follows
Suddenly：According to the parts by weight, the raw material of the component is mixed, level-one crushing is carried out successively and two level crushes, obtain described
Wettable powder.
The dosage form is water dispersible granules；The auxiliary agent can be for c) or d)：C) auxiliary agent is wetting agent, filler and bonding
Agent；D) auxiliary agent is dispersant, wetting agent, filler and binding agent.
In terms of parts by weight, the water dispersible granules can be made of following components；
Any one of the water dispersible granules concretely following B1)-B7)：
B1) in terms of parts by weight, the water dispersible granules are made of following components；
B2) in terms of parts by weight, the water dispersible granules are made (WG1) of following components；
B3) in terms of parts by weight, the water dispersible granules are made (WG2) of following components；
B4) in terms of parts by weight, the water dispersible granules are made (WG3) of following components；
B5) in terms of parts by weight, the water dispersible granules are made (WG4) of following components；
B6) in terms of parts by weight, the water dispersible granules are made (WG5) of following components；
B7) in terms of parts by weight, the water dispersible granules are made (WG6) of following components；
Using the preparation method of conventional water-dispersible grain diameter, specific steps can be as follows for the preparation of the water-dispersible grain diameter：It presses
According to the parts by weight, fluorine thiazole pyrrole ethyl ketone, fludioxonil, dispersant, wetting agent and filler are mixed, crushed, obtaining can wet-milling；
Will described in can wet-milling mix with binding agent and water, granulation, dry, obtain the water dispersible granules.
The dosage form is aqueous emulsion；The auxiliary agent can be thickener, dispersant, wetting agent and water.
In terms of parts by weight, the aqueous emulsion can be made of following components；
In terms of parts by weight, the aqueous emulsion can be specifically made of following components：
Using the preparation method of conventional aqueous emulsion, specific steps can be as follows for the preparation of the aqueous emulsion：According to described heavy
Number is measured, fluorine thiazole pyrrole ethyl ketone, fludioxonil, thickener, dispersant and wetting agent are mixed, obtain oil phase；In stirring bar
It under part, adds water in the oil phase, obtains the aqueous emulsion.
The dosage form is suspending agent；The auxiliary agent is dispersant, wetting agent and water.
In terms of parts by weight, the suspending agent is made of following components：
In terms of parts by weight, the suspending agent can be specifically made of following components：
Using the preparation method of conventional suspending agent, specific steps can be as follows for the preparation of the suspending agent：According to described heavy
Number is measured, fluorine thiazole pyrrole ethyl ketone, fludioxonil, dispersant, wetting agent and water are mixed, grinding obtains the suspending agent.
In above-mentioned all dosage forms, the dispersant can be lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt
(NNO), 3- oleoyls amino -2- methoxy benzenesulfonic acids sodium (LS), sanlose (CMC), alkyl phenol polyoxyethylene ether,
One or more mixtures in ethylene glycol monobutyl ether, polyoxyethylene alkyl ether and neopelex.
The lignin sulfonic acid sodium salt concretely sodium lignin sulfonate (M-14), desugar sodium lignin sulfonate (M-9), sub-
Sulphate pulp waste liquid.Concretely octylphenol polyethylene ethylene oxide (10) ether (OP-10), nonyl phenol gather the alkyl phenol polyoxyethylene ether
Ethylene oxide ether.
When the dispersant is a variety of mixture, concretely mass ratio is 2：1.5 M-9 and CMC, mass ratio 2：
1.5 OP-10 and CMC, mass ratio 2：1.5 or 0.8：1.5 NNO and CMC, mass ratio 2：1.5 LS and CMC, quality
Than being 3：2 M-9 and ethylene glycol monobutyl ether, mass ratio 4：(2.5~3) or 4：2.5 or 4：3 polyoxyethanyl alkyl ether,
The mixture and CMC of nonylphenol polyoxyethylene ether and neopelex composition, wherein, in the mixture, polyoxy second
The mass ratio of base alkyl ether, nonylphenol polyoxyethylene ether and neopelex is 1：2：2.
The wetting agent can sodium alkyl benzene sulfonate, alkyl benzene calcium sulfonate, Negel, fatty alcohol polyoxyethylene ether
(JFC), alkylphenol-polyethenoxy base formaldehyde condensate sulfonate (SOPA), wgwin D30, spaonin powder and bay alcohol radical sulfuric acid
At least one of sodium.
The sodium alkyl benzene sulfonate concretely neopelex.The Negel concretely two fourth
Base sodium naphthalene sulfonate (Nekal BX).
The filler can be one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite and bentonite.
The binding agent is one or more in cyclodextrin, carboxymethyl cellulose, Arabic gum and polyvinyl alcohol.
The thickener is one kind in xanthans, hydroxymethyl cellulose, methylcellulose, aluminum magnesium silicate and polyvinyl alcohol
It is or a variety of.
The present invention also provides combination sterilization compositions described in any one of the above embodiments or compounding described in any one of the above embodiments to kill
Application of the microbial inoculum preparation in controlling plant diseases.
The plant can be melon and fruit and/or vegetables.
The disease can be oomycetes disease, gray mold, early blight, root rot and at least one of dead.
The oomycetes disease includes but not limited to downy mildew, late blight, epidemic disease etc.；The plant disease specifically may be selected from down
State at least one：Cucumber downy mildew, the late blight of potato, target, tomato late blight, gray mold of cucumber, tomato ash
Mildew, capsicum epidemic disease, cucumber root rot and dead of capsicum.
The present invention has the advantages that：
Combination sterilization composition of the present invention has synergy to cucumber root rot bacterium, botrytis cinerea pers and Phytophthora infestans germ
Effect, wherein fluorine thiazole pyrrole ethyl ketone are 1 with fludioxonil mass ratio:Synergy is most strong when 3.
20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 100g a.i./hm of the present invention2、150g a.i./hm2、200g
a.i./hm2Spraying prevention cucumber downy mildew, tomato late blight, the late blight of potato, the effect of capsicum epidemic disease up to 82.4%~
94.2%, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 133.33g a.i./hm2、200g a.i./hm2、266.67g a.i./
hm2Target, the effect of cucumber/graw mold of tomato are prevented up to 80.2%~89.0%；900~1800 times of dilutions fill
Root prevention cucumber root rot, dead of capsicum effect up to 50.5%~65.8%.The composite bactericide quick-acting and lasting effect are good,
Mechanism of action is unique, and action site is more, can effectively prevent vegetables concurrent oomycetes disease, gray mold and early blight, can also have
Dead caused by root rot caused by effect prevention oomycetes, Fusariumsp, rhizoctonia, Phomopsis and Phytophthora capsici, sickle-like bacteria.This is multiple
Preparaton preparation process is simple, at low cost, remarkable in economical benefits, and diseases prevention spectrum is wide, and biological safety is high.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Drug in embodiment and experimental example：96% fludioxonil active compound, 50% fludioxonil WP, 250g/L Fluoxastrobin SC are purchased from
Syngenta (China) Investment Co., Ltd；95% fluorine thiazole pyrrole ethyl ketone active compound, 10% fluorine thiazole pyrrole ethyl ketone OD are purchased from U.S. Du
Company of nation；50% dimethomorph WP, 50% Boscalid WG are purchased from German BASF AG；The mould first of 3% Evil
White AP, purchased from Institute of Plant Protection of the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant." % " in following embodiments is mass percentage.
The bactericidal composition of embodiment 1, the fluorine thiazole pyrrole ethyl ketone of different ratio and fludioxonil is to cucumber root rot bacterium, tomato
Ash arrhizus bacteria and the virulence test of Phytophthora infestans germ
1.1 material to be tested
1.1.1 for examination strain：Cucumber root rot bacterium picks up from Dingzhou City in Hebei Province Zhao Cun townshiies Xi Gandecun for 2015, through separation
Contain Solanum fusarium (Fusarium solani), Phomopsis (Phomopsis sp), P. myriotylum in pathogen identification
(Pythium myriotylum), Rhizoctonia solani Kuhn (Rhizoctonia solani) and De Shi phytophthoras (Phytophthora
drechsleri).Botrytis cinerea (Botrytis cinerea) picks up from Dingxing County of Hebei province Xian Yu towns Longhua for 2015
Village.Phytophthora infestans germ (Phytophthora infestans) picks up from Dingxing County of Hebei province Xian Yu towns Longhua village for 2015.
1.1.2 for examination culture medium：Potato dextrose agar (PDA) culture medium (potato 200g, glucose 20g, agar
20g, deionized water are settled to 1000mL).Rye sucrose agar (RSA) culture medium (rye 60g, sucrose 20g, agar powder 12g,
Deionized water is settled to 1000m L).
1.1.3 for reagent product：Fluorine thiazole pyrrole ethyl ketone and fludioxonil quality proportioning 9~1 are prepared with active compound:1~9 investigational agent
Agent carries out virulence test.
1.2 test method
1.2.1 colony diameter method measures the virulence of fungicide and bactericidal composition to cucumber root rot bacterium
By 95% fluorine thiazole pyrrole ethyl ketone active compound and 96% fludioxonil active compound acetone solution, then it is diluted to the medicine of series concentration
Liquid (content of acetone is no more than 2%), by liquid:Culture medium (PDA) volume ratio 1:9 ratio, addition dissolve postcooling to 60 DEG C
Mixing in the culture medium of left and right adds in the culture dish of diameter 9cm, and drug containing PDA plate is made, and blank control adds in the corresponding of equivalent
Solvent；The cucumber root rot bacterial strain of preculture 4d with the card punch of a diameter of 5mm along colony edge is beaten and takes bacteria cake, is inoculated into and contains
The PDA culture medium tablet center of series concentration, per 1 ferfas cake of ware, mycelia is face-down, covers ware lid, and often processing is repeated 3 times, and is put
Crossing method is used to measure colony diameter after cultivating 4d in 25 DEG C of incubators.The inhibiting rate of each concentration liquid is calculated, is utilized
DPS7.05 Software of Data Statistics is obtained fluorine thiazole pyrrole ethyl ketone, fludioxonil and its mixture and inhibits the growth of cucumber root rot mycelia
Virulence regression equation (Y=a+bx), EC50Value and related coefficient.
Inhibiting rate (%)=(control bacterium colony increases straight for (control bacterium colony increases diameter-processing bacterium colony and increases diameter) × 100/
Diameter)
Colony diameter method measures test method of the bactericidal composition to the virulence of botrytis cinerea and Phytophthora infestans germ
With cucumber root rot bacterium.
1.2.2 the virulence synergist of fungicide compounding
It is calculated according to Wadley (1967) formula and does not have to the mixed coefficient of synergism of medicament：
SR=EC50(th)/EC50(ob)
A, b represent two kinds of fluorine thiazole pyrrole ethyl ketone (A), fludioxonil (B) fungicide shared ratio in the mixture in above formula；
EC50(A) and EC50(B) the actual observation EC of A and B is represented respectively50Value, EC50(th) represent that two kinds of fungicide of A, B are mixed by a ﹕ b
Theoretical EC afterwards50Value, EC50(ob) the mixed actual observation EC of a ﹕ b are pressed for two kinds of fungicide of A, B50Value.SR>1.5, synergy；
SR<0.5, antagonism；SR represents to be added between 0.5 and 1.5.
2. result of the test
As known from Table 1, fluorine thiazole pyrrole ethyl ketone inhibits the synergy of cucumber root rot bacterium mycelia growth to match with fludioxonil mixture
1：Coefficient of synergism is more than 1.5 in the range of (0.2~8), has preferable synergistic effect, wherein fluorine thiazole pyrrole ethyl ketone and fludioxonil
Mass ratio is 1:Synergy is most strong when 3.
1 fluorine thiazole pyrrole ethyl ketone of table is with fludioxonil mixture to the virulence synergist of cucumber root rot bacterium
2 fluorine thiazole pyrrole ethyl ketone of table is with fludioxonil mixture to the virulence synergist of botrytis cinerea
Note：It is measured in PDA culture medium using mycelial growth rate method.
As known from Table 2, fluorine thiazole pyrrole ethyl ketone inhibits the synergy of botrytis cinerea mycelia growth to match with fludioxonil mixture
1：Coefficient of synergism is more than 1.5 in the range of (0.25~6), has preferable synergistic effect, wherein fluorine thiazole pyrrole ethyl ketone is with coughing up bacterium
Nitrile mass ratio is 1:Synergy is most strong when 3.
3 fluorine thiazole pyrrole ethyl ketone of table is with fludioxonil mixture to the virulence synergist of Phytophthora infestans germ
Note：It is measured on RSA culture mediums using mycelial growth rate method.
As known from Table 3, fluorine thiazole pyrrole ethyl ketone inhibits the synergy of Phytophthora infestans germ mycelia growth to match with fludioxonil mixture
1：Coefficient of synergism is more than 1.5 in the range of (0.25~6), has preferable synergistic effect, wherein fluorine thiazole pyrrole ethyl ketone is with coughing up bacterium
Nitrile mass ratio is 1:Synergy is most strong when 3.
Preparation, quality testing and the control effect of embodiment 2, fluorine thiazole pyrrole ethyl ketone and fludioxonil wettable powder
First, the preparation of fluorine thiazole pyrrole ethyl ketone fludioxonil wettable powder
1st, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1 is prepared:3, w/w)
Raw material is divided into each group shown in WP1 in table 4 and prepares wettable powder, is as follows：
95% fluorine thiazole pyrrole ethyl ketone active compound 5.26kg, 96% fludioxonil active compound 15.63kg is taken, adds in 2kg M-9,1.5kg
CMC, 5kg neopelex, 70.61kg fine particle calcium carbonates, by being mixed, into level-one crushing, (grain fineness reaches 80
Mesh)；After row is mixed again, (grain fineness reaches 325 mesh) is crushed into two level, product is packaged as, obtains 20% fluorine thiazole
Pyrrole ethyl ketone fludioxonil WP (1:3, w/w).
2nd, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (2 is prepared:1, w/w)
Each raw material is only replaced with the corresponding components of WP2 in table 4 by preparation method with 1.
3rd, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1 is prepared:1, w/w)
Each raw material is only replaced with the corresponding components of table 4WP3 by preparation method with 1.
4th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1 is prepared:2, w/w)
Each raw material is only replaced with the corresponding components of WP4 in table 4 by preparation method with 1.
5th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1 is prepared:4, w/w)
Each raw material is only replaced with the corresponding components of WP5 in table 4 by preparation method with 1.
6th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1 is prepared:5, w/w)
Each raw material is only replaced with the corresponding components of WP6 in table 4 by preparation method with 1.
4 WP1-WP6 components of table
2nd, the quality testing of fluorine thiazole pyrrole ethyl ketone fludioxonil WP
1) active constituent content：Utilize the content of liquid chromatography for measuring fluorine thiazole pyrrole ethyl ketone and fludioxonil, the results showed that
The content of active ingredient is all higher than 20.0% in 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonils WP.
2) wettability determination：It is carried out according to GB/T5451-2001 pesticide wettable wettability determinations method.1-6 fluorine
The MEBO ribbon gauze of thiazole pyrrole ethyl ketone fludioxonil WP is 55s.
3) measure of suspensibility：It is carried out according to GB/T14825-2006 pesticide wettable suspensibilities assay method.1-6
The suspensibility of fluorine thiazole pyrrole ethyl ketone fludioxonil WP is up to more than 85%.
4) measure of fineness：According to the wet screening in GB/T16150-1995 pesticide wettable fineness assay methods into
Row.Pass through 325 mesh standard sieve (i.e. grain sizes<44 μm) 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonil WP up to 98.5%.
5) measure of moisture：It is carried out according to azeotropic distillation method in GB/T1600-2001 pesticide moisture assay methods.1-6 fluorine
Moisture≤1.8% in thiazole pyrrole ethyl ketone fludioxonil WP.
6) measure of mobility：It is carried out according to international analysis of agricultural drugs coordination board (CIPAC) method.1-6 fluorine thiazole pyrroles
The flow index of ethyl ketone fludioxonil WP is 1.
7) measure of foaming characteristic：It is carried out according to international analysis of agricultural drugs coordination board (CIPAC) method.1-6 fluorine thiazole pyrroles
The foaming volume of ethyl ketone fludioxonil WP is 5mL, meets national standard.
8) measure of thermodynamic stability：It is carried out according to international analysis of agricultural drugs coordination board (CIPAC) method.1-6 fluorine thiophenes
Resolution ratio≤5% of active ingredient, meets national standard after the storage of azoles pyrrole ethyl ketone fludioxonil WP heat.
3rd, cucumber downy mildew, tomato late blight, the late blight of potato, target, cucumber/tomato gray mould are prevented
Disease, cucumber root rot and the effect of dead of capsicum epidemic disease, capsicum
1st, the cucumber downy mildew test of pesticide effectiveness is prevented
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》In " the bactericidal agent for preventing and treating cucumber downy mildew test of pesticide effectiveness
Criterion " is tested.Cucumber variety is " excellent 35 " in Tianjin, Cultivation in Greenhouse, field planting on October 15th, 2015, a ridge duplicate rows, ridge
Away from 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg, powdery mildew of cucumber occurs over the years.Experimental field water
Fertile condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Often 4 repetitions of processing, totally 24
Cell, random alignment, plot area about 18m2。
Start to spray before cucumber downy mildew naturally-occurring, share medicine 3 times, interval is 7 days.Spraying time is respectively
On March 5th, 2016, March 12, March 19.It is uniformly sprayed using Jacto-HD400 types hand sprayer.It is with liquid volume
900L/hm2。
(on March 26th, 2016) investigation morbidity 7 days after last 1 dispenser.Per random 4 sampling surveys of cell, every tune
2 plants are looked into, the whole blades of every plant of investigation, the percentage that entire blade area is accounted for by lesion area on each blade divides sick grade.0 grade：
Disease-free spot；1 grade：Scab accounts for less than 5% entire leaf area；3 grades：Scab accounts for the 6%~10% of entire leaf area；5 grades：Scab
Account for the 11%~25% of entire leaf area；7 grades：Scab accounts for the 26%~50% of entire leaf area；9 grades：Scab accounts for entire blade face
More than 51% long-pending.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index, preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 5,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WP a.i./hm2Foliage-spray is to cucumber
Downy mildew has good control effect (89.4%~93.8%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP150g
a.i./hm2Preventive effect for 91.5%, be significantly higher than and spray 50% dimethomorph WP 375g a.i./hm2Control effect, with
10% fluorine thiazole pyrrole ethyl ketone OD 25g a.i./hm2Control effect it is suitable.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP
100g a.i./hm2Preventive effect for 89.4%, also significantly greater than spray 50% dimethomorph WP 375g a.i./hm2Preventive effect.
Show when preceding or just generation occurs for cucumber downy mildew, using 20% 100~200g of fluorine thiazole pyrrole ethyl ketone fludioxonil WP
a.i./hm2Foliage-spray has preferable control effect.
5 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of cucumber downy mildew
Note：With after column data, indifference is notable (P=0.05) between identical lowercase letter data.Similarly hereinafter.
2nd, the tomato late blight test of pesticide effectiveness is prevented
In Dingxing County of Hebei province Xian Yu towns Longhua village, tomato concentrates producing region, tomato late blight over the years is selected to occur more serious
Heliogreenhouse is tested.Tomato variety is prosperous for Prose.On October 15th, 2015 is colonized, line-spacing 1m, spacing in the rows 0.3m, and preceding stubble is
Tomato, anniversary plantation.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg.Experimental field all cell water and fertilizer conditions are preferable,
Cultivation management condition (soil types, fertilising, farming, watering etc.) uniformity, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil (1:3, w/w) WP dispensers dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Often 4 repetitions of processing, totally 24
Cell, random alignment, plot area 18m2。
Premorbid starts to spray, and shares medicine 3 times, and interval is 7 days.Spraying time is respectively April 18, April in 2016
25 days, May 2.It is uniformly sprayed using Jacto-HD400 types hand sprayer.It is 900L/hm with liquid volume2.It applies for last 1 time
(on May 9th, 2016) investigates incidence 7 days after medicine.Per random 5 sampling surveys of cell, every is investigated 2 plants, every plant of investigation
Whole compound leaves, the percentage that entire compound leaf area is accounted for by lesion area on each compound leaf divide sick grade.0 grade：Disease-free spot；1 grade：Scab
Account for less than 5% entire compound leaf area；3 grades：Scab accounts for the 6%~10% of entire compound leaf area；5 grades：Scab accounts for entire compound leaf
The 11%~25% of area；7 grades：Scab accounts for the 26%~50% of entire compound leaf area；9 grades：Scab accounts for entire compound leaf area
More than 51%.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index, preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 6,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 50~100g of WP a.i./hm2Foliage-spray is to tomato
Late blight has good control effect (89.0%~94.2%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g
a.i./hm2Preventive effect for 94.2%, be significantly higher than and spray 50% dimethomorph WP 375g a.i./hm2And 10% fluorine thiazole pyrrole
Ethyl ketone OD 25g a.i./hm2Control effect.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 150g a.i./hm2It is anti-
It is 91.6% to imitate, and is significantly higher than and sprays 50% dimethomorph WP 375g a.i./hm2Preventive effect.Spray 20% fluorine thiazole pyrrole second
Ketone fludioxonil WP 75g a.i./hm2Preventive effect for 89.0%, with spraying 50% dimethomorph WP 375g a.i./hm2And
10% fluorine thiazole pyrrole ethyl ketone OD 25g a.i./hm2Control effect it is suitable.Show before tomato late blight occurs or just to occur
When, using 20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WP a.i./hm2Foliage-spray has preferable preventive effect.
6 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of tomato late blight
3rd, the late blight of potato test of pesticide effectiveness is prevented
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》In " bactericidal agent for preventing and treating late blight of potato drug effect try
Test criterion " it is tested.Local susceptible variety is Potato Cultivars " Favorita ".Experimental plot is located at Hebei province Weichang gram Le
Small reed ditch village of ditch township, plantation on April 25th, 2016, line-spacing 0.6m, spacing in the rows 0.3m, the late blight of potato occur over the years.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Often 4 repetitions of processing, totally 24
Cell, random alignment.Plot area 54m2If protection row.
Start to spray before field natural occurrence, share medicine 3 times, 7~10 days intervals.Spraying time is respectively June 20
Day, June 27, July 4.It is fine during dispenser.It is sprayed, sprayed uniformly week using Jacto-HD400 types hand sprayer
It arrives.It is 900L/hm with liquid volume2.It is influenced during entire experiment without harsh weather.
(on July 11st, 2016) investigates incidence 7 days after last time dispenser.Per cell 5 sampling surveys of diagonal,
Every is investigated 2 plants, and the whole compound leaves of every plant of investigation, the percentage that entire compound leaf area is accounted for by lesion area on each compound leaf divides disease
Grade.0 grade：Disease-free spot；1 grade：Scab accounts for less than 5% entire compound leaf area；3 grades：Scab account for entire compound leaf area 6%~
10%；5 grades：Scab accounts for the 11%~25% of entire compound leaf area；7 grades：Scab accounts for the 26%~50% of entire compound leaf area；9
Grade：Scab accounts for more than 51% entire compound leaf area.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index and preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 7,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WP a.i./hm2Foliage-spray is to Ma Ling
Potato late blight has good control effect (82.4%~90.6%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g
a.i./hm2Preventive effect for 90.6%, be significantly higher than and spray 50% dimethomorph WP 375g a.i./hm2And 10% fluorine thiazole pyrrole
Ethyl ketone OD 25g a.i./hm2Control effect.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 150g a.i./hm2It is anti-
It is 86.8% to imitate, with spraying 10% fluorine thiazole pyrrole ethyl ketone OD 25g a.i./hm2And 50% dimethomorph WP 375g a.i./hm2
Preventive effect it is suitable.Show when preceding or just generation occurs for the late blight of potato, using 20% fluorine thiazole pyrrole ethyl ketone fludioxonil
WP100~200g a.i./hm2Foliage-spray has preferable preventive effect.
7 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of the late blight of potato
4th, the target test of pesticide effectiveness is prevented
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》In " bactericidal agent for preventing and treating target drug effect try
Test criterion " it is tested.Local susceptible variety is Potato Cultivars " Favorita ".Experimental plot is located at Hebei province Weichang gram Le
Small reed ditch village of ditch township, plantation on April 25th, 2016, line-spacing 0.6m, spacing in the rows 0.3m, target occur over the years.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：250g/L Fluoxastrobin SC, dispenser dosage are 150g a.i./hm2；
50% fludioxonil WP, dispenser dosage are 100g a.i./hm2；Clear water is sprayed in blank control.4 repetitions are often handled, totally 24 cells,
Random alignment.Plot area 54m2If protection row.
Start to spray before field natural occurrence, share medicine 3 times, 7~10 days intervals.Spraying time is respectively June 20
Day, June 27, July 4.It is fine during dispenser.It is sprayed, sprayed uniformly week using Jacto-HD400 types hand sprayer
It arrives.It is 900L/hm with liquid volume2.It is influenced during entire experiment without harsh weather.
(on July 11st, 2016) investigates incidence 7 days after last time dispenser.Per cell 5 sampling surveys of diagonal,
Every is investigated 2 plants, and the whole compound leaves of every plant of investigation, the percentage that entire compound leaf area is accounted for by lesion area on each compound leaf divides disease
Grade.0 grade：Disease-free spot；1 grade：Scab accounts for less than 5% entire compound leaf area；3 grades：Scab account for entire compound leaf area 6%~
10%；5 grades：Scab accounts for the 11%~25% of entire compound leaf area；7 grades：Scab accounts for the 26%~50% of entire compound leaf area；9
Grade：Scab accounts for more than 51% entire compound leaf area.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index and preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 8,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WP a.i./hm2Foliage-spray is to Ma Ling
Potato early blight has good control effect (81.6%~89.0%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g
a.i./hm2Preventive effect for 89.0%, be significantly higher than and spray 250g/L Fluoxastrobin SC 150g a.i./hm2With 250g/L Fluoxastrobins
SC 150g a.i./hm2Control effect.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 150g a.i./hm2Preventive effect
It is 85.9%, with spraying 250g/L Fluoxastrobin SC 150g a.i./hm2Preventive effect it is suitable, be significantly higher than 50% fludioxonil WP
100g a.i./hm2Preventive effect.Show when preceding or just generation occurs for target, using 20% fluorine thiazole pyrrole ethyl ketone
Fludioxonil 150~200g of WP a.i./hm2Foliage-spray has preferable preventive effect.
8 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of target
5th, the capsicum epidemic disease test of pesticide effectiveness is prevented
Medicine inspecting institute of the experimental evidence Ministry of Agriculture《Pesticide field efficacy medicine test criterion》In " bactericidal agent for preventing and treating capsicum epidemic disease drug effect try
Test criterion " it carries out.Susceptible variety is capsicum variety " the ears of an ox or cow king ".Experimental plot is located at Dingxing County of Hebei province and consolidates cities and towns Bei Zhuantoucun,
For greenhouse cultivation, line-spacing 0.8m, spacing in the rows 0.3m.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage is 50g a.i./hm2、
75g a.i./hm2、100g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD 25g a.i./hm2；50% alkene acyl
Quinoline WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.4 repetitions are often handled, totally 24 cells, it is random to arrange
Row, plot area 24m2。
Premorbid starts to spray, and shares medicine 3 times, and interval is 7 days.Spraying time is respectively May 4, May 11 in 2016
Day, May 18.It is uniformly thoughtfully sprayed using Jacto-HD400 types hand sprayer.It is 900L/hm with liquid volume2。
(on May 25th, 2016) is investigated 7 days after last 1 dispenser.Per random 5 sampling surveys of cell, every tune
Look into 10 plants.Stage division：0 grade：It is disease-free；1 grade：Rhizome portion disease-free spot, indivedual branches or fruit morbidity；3 grades：Rhizome portion is slightly sent out
Disease or the morbidity of the following branch of half or fruit；5 grades：The morbidity of rhizome portion moderate or branch more than half or fruit morbidity；7 grades：Root
Stem seriously falls ill, and the big portion of plant is wilting；9 grades：Plant all wilting death.It sporadicly falls ill before first time dispenser, state of an illness radix
It is denoted as zero.Each processing state of an illness is recorded according to more than stage division, calculates incidence, disease index, preventive effect.Data use SPSS
Fisher ' s least significant differences (LSD) measure for statistical analysis in 20.0 softwares.
Diseased plant rate (%)=(diseased plant number/investigation total strain number) × 100
Disease index=Σ (diseased plant numbers at different levels × relative disease value of series)/(investigation total strain number × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 9,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WP a.i./hm2Foliage-spray is to capsicum
Epidemic disease has good control effect (89.1%~92.6%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g
a.i./hm2Preventive effect for 92.6%, be significantly higher than and spray 50% dimethomorph WP 375g a.i./hm2And 10% fluorine thiazole pyrrole
Ethyl ketone OD 25g a.i./hm2Control effect.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~150g of WP a.i./
hm2Preventive effect for 89.1%~90.4%, be significantly higher than and spray 50% dimethomorph WP 375g a.i./hm2Preventive effect, with spray
Apply 10% fluorine thiazole pyrrole ethyl ketone OD 25g a.i./hm2Control effect it is suitable.Show before capsicum epidemic disease occurs or just to occur
20% fluorine thiazole pyrrole ethyl ketone fludioxonil 150~200g of WP a.i./hm of Shi Caiyong2Foliage-spray has preferable control effect.
9 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of capsicum epidemic disease
6th, the gray mold of cucumber test of pesticide effectiveness is prevented
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》GB/T17980.28-2000 " bactericidal agent for preventing and treating vegetables
Dish gray mold test of pesticide effectiveness criterion " is tested.
Experimental plot is located at Dingxing County of Hebei province Xian Yu towns Longhua village, cucumber variety for " excellent 35 " in Tianjin, Cultivation in Greenhouse,
October 14 in 2015 is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg,
Cucumber root rot occurs over the years.Experimental field water and fertilizer condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage for 133.33g a.i./
hm2、200g a.i./hm2、266.67g a.i./hm2.Comparison medicament：50% Boscalid WG, dispenser dosage are 300g
a.i./hm2；50% fludioxonil WP, dispenser dosage are 100g a.i./hm2；Clear water is sprayed in blank control.4 repetitions are often handled, altogether
24 cells, random alignment, plot area 18m2。
Premorbid starts to spray, and shares medicine 3 times, and interval is 7 days.Spraying time is respectively March 6, March 13 in 2016
Day, March 20.It is uniformly thoughtfully sprayed using Jacto-HD400 types hand sprayer.It is 900L/hm with liquid volume2。
(on March 27th, 2016) is investigated 7 days after last 1 dispenser.Incidence is investigated, takes at random at 5 points per cell,
Whole fruits of every 2 plants of investigation record disease fruit number caused by gray mold.Gray mold of cucumber is investigated as unit of fruit：0 grade：Nothing
Scab；1 grade：Residual flower morbidity；3 grades：It falls ill in areola portion；5 grades：Scab length accounts for less than 10% fruit；7 grades：Scab length accounts for
The 11%~25% of fruit；9 grades：Scab length accounts for more than 26% fruit.Diseased fruit rate, disease index are calculated according to more than stage division
And control effect, data use Fisher ' s least significant differences (LSD) in 20.0 softwares of SPSS to measure progress statistical
Analysis.
Diseased fruit rate (%)=(the total fruit number of disease fruit number/investigation) × 100
Disease index=Σ (disease fruit number × relative disease value of series at different levels)/(investigating total fruit number × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 10,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 133.33~266.67g of WP a.i./hm2It sprays on blade face
Applying has gray mold of cucumber good control effect (80.2%~85.9%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP
266.67g a.i./hm2Preventive effect for 85.9%, with spraying 50% Boscalid WG 300g a.i./hm2And 50% fludioxonil
WP 100g a.i./hm2Control effect.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g a.i./hm2Preventive effect
It is 82.0%, substantially less than sprays 50% Boscalid WG 300g a.i./hm2And spray 50% fludioxonil WP 100g
a.i./hm2Control effect.When showing that preceding or just generation occurs for gray mold of cucumber, bacterium is coughed up using 20% fluorine thiazole pyrrole ethyl ketone
Nitrile WP133.33~266.67g a.i./hm2Carry out foliage-spray has preferable preventive effect to gray mold of cucumber.
10 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of gray mold of cucumber
7th, the graw mold of tomato test of pesticide effectiveness is prevented
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》GB/T17980.28-2000 " bactericidal agent for preventing and treating vegetables
Dish gray mold test of pesticide effectiveness criterion " is tested.Tomato variety is conventional variety " east victory hooligan ".Experimental plot is located at Hebei province Xu
Water county Gao Lin villages and small towns Tian Cunpu villages, field planting on November 10th, 2015.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage for 133.33g a.i./
hm2、200g a.i./hm2、266.67g a.i./hm2.Comparison medicament：50% Boscalid WG, dispenser dosage are 300g
a.i./hm2；50% fludioxonil WP, dispenser dosage are 100g a.i./hm2；Clear water is sprayed in blank control.4 repetitions are often handled, altogether
24 cells, random alignment, plot area 18m2。
Premorbid starts to spray, and shares medicine 3 times, and interval is 7 days.Spraying time is respectively on 2 4th, 2016,2 months 11
Day, 18 days 2 months.It is uniformly thoughtfully sprayed using Jacto-HD400 types hand sprayer.It is 900L/hm with liquid volume2。
(on March 25th, 2016) is investigated 7 days after last 1 dispenser.Incidence is investigated, takes at random at 5 points per cell,
Whole fruits of every 2 plants of investigation record disease fruit number caused by gray mold.Grey mold disease fruit grade scale is：0 grade：Disease-free spot；1
Grade：Remain petal morbidity or column cap morbidity；3 grades：Sepal rots or column cap morbidity spreads to areola portion；5 grades：There is infiltration in areola portion
Spot but without mould layer；7 grades：There is mould layer in areola portion but does not expand to other positions；9 grades：The mould layer in areola portion expands to other positions.Root
Upper stage division calculates diseased fruit rate, disease index and control effect according to this, and data use in SPSS20.0 softwares Fisher ' s most
Low significant difference (LSD) measures for statistical analysis.
Diseased fruit rate (%)=(the total fruit number of disease fruit number/investigation) × 100
Disease index=Σ (disease fruit number × relative disease value of series at different levels)/(investigating total fruit number × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 11,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 133.33~266.67g of WP a.i./hm2It sprays on blade face
Applying has graw mold of tomato good control effect (82.1%~86.2%).Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil
WP266.67g a.i./hm2Preventive effect for 86.2%, with spraying 50% Boscalid WG 300g a.i./hm2And spray 50%
Fludioxonil WP 100g a.i./hm2Control effect it is suitable.Spray 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP 200g
a.i./hm2Preventive effect for 84.3%, with spraying 50% Boscalid WG 300g a.i./hm2And spray 50% fludioxonil WP
100g a.i./hm2Control effect it is suitable.When showing that preceding or just generation occurs for graw mold of tomato, using 20% fluorine thiazole pyrrole second
Ketone fludioxonil WP133.33~266.67g a.i./hm2Carrying out foliage-spray has preferable control effect.
11 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of graw mold of tomato
8th, the cucumber root rot test of pesticide effectiveness is prevented
Experimental plot is located at Dingzhou City in Hebei Province Zhao Cun townshiies Xi Gandecun, and cucumber variety is " capital is green ", Cultivation in Greenhouse, and 2015
October 14 in year is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg, cucumber
Root rot occurs over the years.Experimental field water and fertilizer condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage for 1800 times of liquid, 1200 times
Liquid, 900 times of liquid.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage are 600 times of liquid；50% fludioxonil WP, dispenser agent
It measures as 4500 times of liquid, blank control filling clear water.4 repetitions are often handled, totally 24 cells, random alignment, plot area 24m2.
Before cucumber root rot occurs, every plant of irrigation liquid 250mL.Every each processing root rot of 15d investigation, a situation arises after medicine, record disease
Strain number calculates control effect according to diseased plant rate.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares
It measures for statistical analysis.
As known from Table 12,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 900~1800 times of liquid irrigating roots of WP have cucumber root rot
Good control effect (54.8%~65.8%) is significantly higher than 10% 3600 times of liquid of fluorine thiazole pyrrole ethyl ketone OD, 50% fludioxonil
The control effect of 4500 times of liquid of WP and the mould 650 times of liquid of first frost AP of 3% Evil.Show that cucumber root rot uses 20% fluorine before occurring
Thiazole pyrrole ethyl ketone fludioxonil 900~1800 times of liquid irrigating roots of WP have preferable control effect.
12 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP of table is to the control effect of cucumber root rot
9th, dead test of pesticide effectiveness of capsicum is prevented
Susceptible variety is capsicum variety " the ears of an ox or cow king ".Experimental plot is located at Dingxing County of Hebei province and consolidates cities and towns Bei Zhuantoucun, is big
Canopy cultivated peppers, line-spacing 0.8m, spacing in the rows 0.3m.October 14 in 2015 is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Before field planting
Chicken manure 5m is applied per acre3, diammonium hydrogen phosphate 50kg, dead of capsicum is over the years to be occurred.Experimental field water and fertilizer condition is preferable, cultivation management condition
Unanimously, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP (1:3, w/w) dispenser dosage for 1800 times of liquid, 1200 times
Liquid, 900 times of liquid.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage are 3600 times of liquid；50% fludioxonil WP, dispenser agent
It measures as 4500 times of liquid, blank control filling clear water.4 repetitions are often handled, totally 24 cells, random alignment, plot area 24m2。
Before dead generation of capsicum, every plant of irrigation liquid 250mL.Every each handling dead of 15d investigation, a situation arises after medicine,
Diseased plant number is recorded, control effect is calculated according to diseased plant rate.Data use the minimum conspicuousnesses of Fisher ' s in SPSS20.0 softwares poor
Different (LSD) measures for statistical analysis.
As known from Table 13,20% fluorine thiazole pyrrole ethyl ketone fludioxonil 1800~3600 times of liquid irrigating roots of WP have capsicum for dead
Good control effect (50.5%~65.7%), is significantly higher than 10% OD3600 times of liquid of fluorine thiazole pyrrole ethyl ketone, 50% fludioxonil
The control effect of 4500 times of liquid of WP and the mould 650 times of liquid of first frost AP of 3% Evil.Show that dead of capsicum uses 20% fluorine thiophene before occurring
Azoles pyrrole ethyl ketone fludioxonil 900~1800 times of liquid irrigating roots of WP have preferable control effect.
The control effect of 13 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP dead to capsicum of table
Experiments have shown that：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WP100~200g a.i./hm2Foliar spray is to cucumber cream
Mildew, tomato late blight, capsicum epidemic disease, 133.33~266.67g a.i./hm2Foliar spray cucumber/graw mold of tomato has
There is a good preventive and therapeutic effect, 20% fluorine thiazole 900~1800 times of liquid irrigating roots of pyrrole ethyl ketone fludioxonil WP are to cucumber root rot and peppery
Dead of green pepper has good control effect, is applied in being produced in field.
Preparation, quality testing and the control effect of embodiment 3, fluorine thiazole pyrrole ethyl ketone and fludioxonil water dispersible granules
First, the preparation of fluorine thiazole pyrrole ethyl ketone fludioxonil water dispersible granules
1st, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1 is prepared:3, w/w)
Raw material is divided into each group shown in WG1 in table 14 and prepares water dispersible granules, is as follows：
95% fluorine thiazole pyrrole ethyl ketone active compound 5.26kg, 96% fludioxonil active compound 15.63kg is taken, adds in NNO 2kg, CMC
1.5kg, neopelex 5kg, white carbon 68.61kg, through mixing, air-flow crushing obtain can wet-milling, can
Wet-milling adds in kneader mediate simultaneously with 2kg cyclodextrin and 5kg water, and the material of plasticity is made, and is finally sent into this material and squeezes
Comminutor is pressed, is granulated, water dispersible granules product is obtained by drying, screening.
2nd, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (2 is prepared:1, w/w)
Each raw material is only replaced with the corresponding components of WG2 in table 14 by preparation method with 1.
3rd, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1 is prepared:1, w/w)
Each raw material is only replaced with the corresponding components of WG3 in table 14 by preparation method with 1.
4th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1 is prepared:2, w/w)
Each raw material is only replaced with the corresponding components of WG4 in table 14 by preparation method with 1.
5th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1 is prepared:4, w/w)
Each raw material is only replaced with the corresponding components of WG5 in table 14 by preparation method with 1.
6th, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1 is prepared:5, w/w)
Each raw material is only replaced with the corresponding components of WG6 in table 14 by preparation method with 1.
14 WG1-WG6 components of table
2nd, fluorine thiazole pyrrole ethyl ketone fludioxonil WG quality testings
1) active constituent content measuring：Utilize the content of liquid chromatography for measuring fluorine thiazole pyrrole ethyl ketone and fludioxonil.1-6 fluorine
The content of active ingredient is all higher than 20.00% in thiazole pyrrole ethyl ketone fludioxonil WG.
2) determination of moisture：It is carried out according to azeotropic distillation method in GB/T1600-2001 pesticide moisture assay methods.1-6 fluorine thiophenes
Moisture≤1.8% in azoles pyrrole ethyl ketone fludioxonil WG.
3) dispersed measure：Using the dispersibility of long tube test method determination 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonils WG.Pipe
The deposit of bottom is 0.1mL.
4) wettability determination：Using measuring graduates test method determination.The wetability of 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonils WG
Well.
5) disintegrative measures：Into 100mL tool plugs graduated cylinder (interior high 22.5cm, internal diameter 28cm) containing 90mL distilled water in
Sample particle (0.5g, 250~1410 μm) is added at 25 DEG C, the middle part of graduated cylinder is clamped later, clogs bung hole, with the speed of 8r/min
Degree is rotated around center, until sample is disintegrated completely in water.The disintegration time of 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonils WG is
2min。
6) suspension stability：It is measured according to GB/T14825-2006 methods.1-6 fluorine thiazole pyrrole ethyl ketone fludioxonils WG
Suspensibility up to more than 90%.
7) measure of thermodynamic stability：Sample is enclosed in ground wide-mouth bottle, storage 4 weeks in (50 ± 1) DEG C insulating box
Afterwards, 1-6 fluorine thiazole pyrrole ethyl ketone fludioxonil WG active constituent contents are analyzed.Active ingredient resolution ratio is less than 5%.
3rd, the cucumber downy mildew test of pesticide effectiveness is prevented
Experimental plot is located at Dingxing County of Hebei province Xian Yu towns Longhua village, cucumber variety for " excellent 35 " in Tianjin, Cultivation in Greenhouse,
October 14 in 2015 is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg,
Cucumber cucumber downy mildew occurs over the years.Experimental field water and fertilizer condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Totally 12 each processing, is often handled 4 times
It repeats, totally 48 cells, random alignment, plot area about 18m2。
Start to spray before cucumber downy mildew naturally-occurring, share medicine 3 times, interval is 7 days.Spraying time is respectively
On March 5th, 2016, March 12, March 19.It is uniformly sprayed using Jacto-HD400 types hand sprayer.It is with liquid volume
900L/hm2。
(on March 26th, 2016) investigation morbidity 7 days after last 1 dispenser.Per random 4 sampling surveys of cell, every tune
2 plants are looked into, the whole blades of every plant of investigation, the percentage that entire blade area is accounted for by lesion area on each blade divides sick grade.0 grade：
Disease-free spot；1 grade：Scab accounts for less than 5% entire leaf area；3 grades：Scab accounts for the 6%~10% of entire leaf area；5 grades：Scab
Account for the 11%~25% of entire leaf area；7 grades：Scab accounts for the 26%~50% of entire leaf area；9 grades：Scab accounts for entire blade face
More than 51% long-pending.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index, preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
The result shows that 20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of WG a.i./hm2Foliage-spray is to cucumber
Downy mildew has good control effect (83.0%~91.0%).
15 20% fluorine thiazole pyrrole ethyl ketone fludioxonil WG of table is to the control effect of cucumber downy mildew
Embodiment 4 prepares fluorine thiazole pyrrole ethyl ketone fludioxonil aqueous emulsion
1st, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil AE (1 is prepared:3, w/w)
20% fluorine thiazole pyrrole ethyl ketone fludioxonil AE (1 is prepared in accordance with the following steps:3, w/w)：
95% fluorine thiazole pyrrole ethyl ketone active compound 5.26kg, 96% fludioxonil active compound 15.63kg is taken, adds in xanthans 1.2kg,
NNO 0.8kg, CMC 1.5kg, neopelex 5kg, by being mixed, under high velocity agitation, by water 70.61kg
It is added in oil phase, forms the aqueous emulsion of favorable dispersibility.After testing, quality testing meets relevant criterion.
2nd, the cucumber downy mildew test of pesticide effectiveness is prevented
Experimental plot is located at Dingxing County of Hebei province Xian Yu towns Longhua village, cucumber variety for " excellent 35 " in Tianjin, Cultivation in Greenhouse,
October 14 in 2015 is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg,
Cucumber cucumber downy mildew occurs over the years.Experimental field water and fertilizer condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil AE (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Totally 12 each processing, is often handled 4 times
It repeats, totally 48 cells, random alignment, plot area about 18m2。
Start to spray before cucumber downy mildew naturally-occurring, share medicine 3 times, interval is 7 days.Spraying time is respectively
On March 5th, 2016, March 12, March 19.It is uniformly sprayed using Jacto-HD400 types hand sprayer.It is with liquid volume
900L/hm2。
(on March 26th, 2016) investigation morbidity 7 days after last 1 dispenser.Per random 4 sampling surveys of cell, every tune
2 plants are looked into, the whole blades of every plant of investigation, the percentage that entire blade area is accounted for by lesion area on each blade divides sick grade.0 grade：
Disease-free spot；1 grade：Scab accounts for less than 5% entire leaf area；3 grades：Scab accounts for the 6%~10% of entire leaf area；5 grades：Scab
Account for the 11%~25% of entire leaf area；7 grades：Scab accounts for the 26%~50% of entire leaf area；9 grades：Scab accounts for entire blade face
More than 51% long-pending.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index, preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
The result shows that 20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of AE a.i./hm2Foliage-spray is to cucumber
Downy mildew has good control effect (83.0%~91.0%).
16 20% fluorine thiazole pyrrole ethyl ketone fludioxonil AE of table is to the control effect of cucumber downy mildew
Embodiment 5 prepares fluorine thiazole pyrrole ethyl ketone fludioxonil suspending agent
1st, 20% fluorine thiazole pyrrole ethyl ketone fludioxonil SC (1 is prepared:3, w/w)
20% fluorine thiazole pyrrole ethyl ketone fludioxonil SC (1 is prepared in accordance with the following steps:3, w/w)：
95% fluorine thiazole pyrrole ethyl ketone active compound 5.26kg, 96% fludioxonil active compound 15.63kg is taken, adds in NNO 2kg,
Using water as medium, active component, auxiliary agent are added in sand milling kettle by CMC1.5kg, neopelex 5kg, water 70.61kg
It mixes and is ground with abrasive media, obtain homogeneous suspension liquid body, liquid is detached into obtained suspending agent with abrasive media.Through inspection
It surveys, quality testing meets relevant criterion.
2nd, the cucumber downy mildew test of pesticide effectiveness is prevented
Experimental plot is located at Dingxing County of Hebei province Xian Yu towns Longhua village, cucumber variety for " excellent 35 " in Tianjin, Cultivation in Greenhouse,
October 14 in 2015 is colonized, a ridge duplicate rows, row spacing 1m, spacing in the rows 0.3m.Chicken manure 5m is applied per acre before field planting3, diammonium hydrogen phosphate 50kg,
Cucumber cucumber downy mildew occurs over the years.Experimental field water and fertilizer condition is preferable, and cultivation management is consistent, Pass Test requirement.
Test medicine：20% fluorine thiazole pyrrole ethyl ketone fludioxonil SC (1:3, w/w) dispenser dosage is 100g a.i./hm2、
150g a.i./hm2、200g a.i./hm2.Comparison medicament：10% fluorine thiazole pyrrole ethyl ketone OD, dispenser dosage for 25g a.i./
hm2；50% dimethomorph WP, dispenser dosage are 375g a.i./hm2；Clear water is sprayed in blank control.Totally 12 each processing, is often handled 4 times
It repeats, totally 48 cells, random alignment, plot area about 18m2.Start to spray before cucumber downy mildew naturally-occurring, share medicine
3 times, interval is 7 days.Spraying time is respectively on March 5th, 2016, March 12, March 19.Using Jacto-HD400 types
Hand sprayer uniformly sprays.It is 900L/hm with liquid volume2。
(on March 26th, 2016) investigation morbidity 7 days after last 1 dispenser.Per random 4 sampling surveys of cell, every tune
2 plants are looked into, the whole blades of every plant of investigation, the percentage that entire blade area is accounted for by lesion area on each blade divides sick grade.0 grade：
Disease-free spot；1 grade：Scab accounts for less than 5% entire leaf area；3 grades：Scab accounts for the 6%~10% of entire leaf area；5 grades：Scab
Account for the 11%~25% of entire leaf area；7 grades：Scab accounts for the 26%~50% of entire leaf area；9 grades：Scab accounts for entire blade face
More than 51% long-pending.
It sporadicly falls ill before first time dispenser, state of an illness radix is denoted as zero.Each processing state of an illness is recorded according to more than stage division, is counted
Calculate incidence, disease index, preventive effect.Data use Fisher ' s least significant differences (LSD) in SPSS20.0 softwares to measure
It is for statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigation total number of sheets) × 100
Disease index=Σ (the disease number of sheets × relative disease value of series at different levels)/(investigation total number of sheets × 9) × 100
Control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
The result shows that 20% fluorine thiazole pyrrole ethyl ketone fludioxonil 100~200g of SC a.i./hm2Foliage-spray is to cucumber
Downy mildew has good control effect (83.0%~91.0%).
17 20% fluorine thiazole pyrrole ethyl ketone fludioxonil SC of table is to the control effect of cucumber downy mildew
Claims (9)
1. a kind of combination sterilization composition, it is characterised in that：It is made of fluorine thiazole pyrrole ethyl ketone and fludioxonil；Wherein, the fluorine
The mass ratio of thiazole pyrrole ethyl ketone and the fludioxonil is 1：(0.2~8).
2. combination sterilization composition according to claim 1, it is characterised in that：The fluorine thiazole pyrrole ethyl ketone coughs up bacterium with described
The mass ratio of nitrile is 1：(0.25~6).
3. combination sterilization composition according to claim 1 or 2, it is characterised in that：The fluorine thiazole pyrrole ethyl ketone with it is described
The mass ratio of fludioxonil is 1：(1~5).
4. a kind of compound sterilizing agent formulation, it is characterised in that：It is combined by the compound sterilizing described in any one of claim 1-3
Acceptable auxiliary agent composition on object and pesticidal preparations.
5. compound sterilizing agent formulation according to claim 4, it is characterised in that：The dosage form choosing of the compound sterilizing agent formulation
From following at least one：Suspending agent, wettable powder, aqueous emulsion, water dispersible granules.
6. compound sterilizing agent formulation according to claim 4 or 5, it is characterised in that：In the compound sterilizing agent formulation, institute
The mass percentage for stating combination sterilization composition is 10%~50%.
7. the compounding described in any one of combination sterilization composition or claim 4-6 described in any one of claim 1-3
Application of the fungicide preparation in controlling plant diseases.
8. application according to claim 7, it is characterised in that：The plant is melon and fruit and/or vegetables；The disease is ovum
Fungus diseases, gray mold, early blight, root rot and at least one of dead；And/or the oomycetes disease is downy mildew, late blight
Disease or epidemic disease.
9. the application according to right 7 or 8, it is characterised in that：The plant disease is selected from following at least one：Cucumber downy mildew
Disease, the late blight of potato, target, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease, cucumber root
Maize ear rot and dead of capsicum.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102459256A (en) * | 2009-04-22 | 2012-05-16 | 纳幕尔杜邦公司 | Solid forms of an azocyclic amide |
| CN104663674A (en) * | 2007-10-23 | 2015-06-03 | 杜邦公司 | Fungicidal Mixtures |
| CN105104386A (en) * | 2015-07-25 | 2015-12-02 | 海利尔药业集团股份有限公司 | Sterilization composition containing hymexazol and fludioxonil |
| CN106719710A (en) * | 2017-01-20 | 2017-05-31 | 广东中迅农科股份有限公司 | A kind of bactericidal composition containing fluorine thiazole pyrrole ethyl ketone |
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2018
- 2018-02-11 CN CN201810141552.2A patent/CN108142424A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663674A (en) * | 2007-10-23 | 2015-06-03 | 杜邦公司 | Fungicidal Mixtures |
| CN102459256A (en) * | 2009-04-22 | 2012-05-16 | 纳幕尔杜邦公司 | Solid forms of an azocyclic amide |
| CN105104386A (en) * | 2015-07-25 | 2015-12-02 | 海利尔药业集团股份有限公司 | Sterilization composition containing hymexazol and fludioxonil |
| CN106719710A (en) * | 2017-01-20 | 2017-05-31 | 广东中迅农科股份有限公司 | A kind of bactericidal composition containing fluorine thiazole pyrrole ethyl ketone |
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