CN108142219B - Fast asexual cuttage propagation method for Polygala crotalarioides - Google Patents
Fast asexual cuttage propagation method for Polygala crotalarioides Download PDFInfo
- Publication number
- CN108142219B CN108142219B CN201711427977.1A CN201711427977A CN108142219B CN 108142219 B CN108142219 B CN 108142219B CN 201711427977 A CN201711427977 A CN 201711427977A CN 108142219 B CN108142219 B CN 108142219B
- Authority
- CN
- China
- Prior art keywords
- cutting
- cuttage
- plant
- rooting
- propagation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to the technical field of plant asexual cuttage rapid propagation, in particular to a cuttage rapid propagation method of Polygala crotalarioides, which comprises the following steps: (1) collecting cutting slips, (2) cutting period, (3) preparing a substrate, (4) preparing the cutting slips, (5) cutting, (6) cultivating, and (7) transplanting. The cutting mode of the invention adopts flat cutting, the ear making mode adopts flat cutting with the upper and lower surfaces being planes, the rooting solution adopts KT-30+ NAA + Bacteria combined hormone treatment solution, the substrate adopts river sand, the survival rate is high, the rooting rate is high, the root system grows well, the rooting rate and the survival rate both reach more than 93.8 percent, and the bottle seedling reaches 96.7 percent. The method for asexual cutting propagation of Polygala crotalarioides has the advantages of simple operation, low cost, high survival rate and high rooting rate, and can be harvested after three to five years of Polygala crotalarioides cultivation generally, so that a high profit margin is obtained, and the method has important practical significance for developing forest economy, increasing the income of farmers and keeping rural stability.
Description
Technical Field
The invention relates to the technical field of plant asexual cuttage rapid propagation, in particular to a cuttage rapid propagation method of Polygala crotalarioides.
Background
Polygala crotalarioides line transliteration (Va Wen: Nya Miex Tiang), alias: YOU, Nera, is a perennial herb of Polygala crotalarioides Buch. The whole plant is used as the medicine, has the best root effect, and is favored to grow on laterite sunny hills, thin tree tall grass communities and shrub grass slopes with the elevation of 1300 plus and 2000 m. The Chinese medicinal composition is distributed in Afghan Va mountain areas, Yun county, Shuangjiang and the like, is a rare Chinese medicinal herb in Va nationality in Yunnan Va, has the medicinal history for more than 300 years according to introduction of the Cao doctor of the Va nationality, has the functions of stimulating appetite, strengthening tendons and bones, enhancing sexual function and resisting fatigue, and can make eyebrow hairs beautiful and long-lasting after long-term administration, and has the effects of beautifying and brightening and eliminating nevus spots. In the fighting cattle competition, the earliest Va nationality uses Polygala crotalarioides as a 'force-exerting medicine' to feed fighting cattle, so that physical strength is doubled and fighting will be raised. The product has warm and nontoxic properties, has numb, spicy and diced flavor, and has effects of heart, kidney and swelling channels, tranquilizing mind, improving intelligence, eliminating phlegm, relieving swelling, promoting blood circulation, relieving pain, invigorating kidney, tonifying yang, relieving fatigue, resisting stress, and inducing male hormone. Pharmacological tests and clinical researches show that the compound has various physiological activities of immunity enhancement, immunologic adjuvant, antipsychotic, blood fat regulation, anticoagulation, antithrombotic, antitumor and the like. It can be used for treating sexual hypofunction such as sexual impotence, premature ejaculation, cough and asthma due to kidney deficiency, arthralgia due to wind-cold-dampness, lymphoid tuberculosis and tuberculosis, rheumatic numbness, insomnia, dreaminess, difficulty in expectoration, senile dementia, amnesia, palpitation, swelling and pain of breast, female infertility due to cold womb, and clear and thin womb.
The main components separated from the plants at present are triterpenoid saponin compounds, ketones, xanthene ketoside compounds, oleanolic acid type saponins, oligosaccharide esters, alkaloids, flavonoids, phenylpropanoids, coumarins, male hormone-like compounds and the like, and other organic components and inorganic substances. According to the pharmacological research of the strengthening effect of Va nationality herbal medicine ' YOU ' of YOU ' taught by Zhang Fu Li Ming torch Sun, the medicine is proved to have the effects of obviously promoting the development of testis of young male mice and the growth of atrophic accessory gonadal organs of castrated male rats, and can obviously enhance the swimming endurance, the normal pressure anoxia resistance and the low temperature resistance of the mice. "YOUYUNIANG" has effects in improving anti-stress ability of organism and has male hormone-like effect.
Due to outstanding drug effect, people strive for mining, the resources of the origin are seriously exhausted, the resources are nearly exhausted, and the survival rate of wild domestication is low, so that the research on the rapid propagation technology of the nianhua mother's health to protect the rare traditional Chinese medicine variety is an important subject to be solved urgently.
At present, only domestic research reports on Polygala crotalarioides mainly focus on two aspects of medicinal efficacy and medicinal components, such as Polygala crotalarioides film agent disclosed by Chinese patent application No. 2009100945950 and Polygala crotalarioides effervescent agent disclosed by Chinese patent application No. 2009100945861, but the research on the cultivation, propagation, breeding and the like of the Polygala crotalarioides is less, and particularly, the research on the asexual cuttage experimental propagation technology is not reported.
The cutting seedling grows faster than the seedling in the early stage, the propagation coefficient is high, the cost is low, the excellent characters of the female parent can be kept, and the seed collection is difficult. Under the condition of limited experimental equipment and resources, the research on the asexual cutting propagation of Polygala crotalarioides has innovative significance and practical significance.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a fast asexual cutting propagation method of Polygala crotalarioides with simple operation, low cost, high survival rate and high rooting rate.
In order to achieve the purpose, the invention adopts the following technical scheme:
the asexual cutting propagation method of the Polygala crotalarioides comprises the following steps:
(1) collecting cutting slips
Cutting branches of the same year from a strong, nondestructive and disease and pest-free wild Polygala crotalarioides plant, and respectively cutting two cuttings of a stem section with a top bud and a stem section with leaves;
(2) period of cuttage
The cuttage of Polygala crotalarioides can be carried out in both spring and summer;
(3) substrate preparation
Sterilizing a cutting matrix by using 3-7% potassium permanganate solution before cutting, filling the cutting matrix into a cutting bed, leveling, watering thoroughly, and preparing for cutting propagation, wherein the thickness of the matrix is 15-20 cm;
(4) preparation of cutting shoots
Soaking the bottoms of the cuttings with a rooting solution for 25-35 minutes, wherein the liquid level of the soaking solution reaches 1-2cm of the bottoms of the cuttings during soaking;
(5) cutting
After the cuttage is finished, cuttage can be carried out, the row spacing of the cuttage is 4-7cm, the plant spacing is 4-6cm, a wood stick punching method is used, the cutting slips are placed in holes, the cutting slips are preferably 2/3 immersed in the soil, then the cutting slips are compacted by hands, the bottoms of the cutting slips are in close contact with a cuttage matrix, then the water is poured, an arch-shaped support is placed on the surface of a cuttage bed, an agricultural plastic film is covered on the arch-shaped support, and then a sunshade net with the light transmittance of 90% is covered on the arch-shaped support;
(6) cultivation of
Spraying water into the cuttage bed for 1-3 times every day to keep the relative humidity in the arched shed at 80-95%, frequently checking and recording the rooting condition in daily management, weeding at any time, preventing and treating plant diseases and insect pests in time and removing diseased plants, and rooting and germinating 10-18 days after cuttage to become cuttage seedlings;
(7) transplanting
After the cutting seedling survives, planting in a wild-like manner, transplanting in an artificially planted pine forest, and planting with the bed height of 20cm, the bed width of 1-1.2m, the row spacing of 20-25cm and the branch spacing of 15-20 cm.
Preferably, the scissoring method of the two cutting shoots with the apical bud stem segment and the leaf stem segment in the step (1) comprises the following steps:
1) cutting the stem with the top bud: selecting terminal bud segments of mother good branches of the mother, cutting slips according to the standard of reserving 3-5 nodes and 2-4cm in length, reserving terminal buds for each slip, and cutting the lower incision into a plane;
2) cutting the stem with leaves: cutting the remaining branches of the Polygala crotalarioides with 2-3 nodes and length of 2-4cm, cutting axillary buds 2-4mm away from the upper nodes of the cutting into a plane, and cutting the lower cuts into a plane.
Preferably, in the process of making the cutting in the step (1), the leaf surfaces are continuously sprayed with water to prevent the ear strips from losing water.
Preferably, the local air temperature in the cuttage period of the step (2) is 22-27 ℃.
Preferably, the matrix of step (3) is river sand.
Preferably, the rooting solution of step (4) is: KT-30+ NAA + Bactericin.
Preferably, the incubation period of step (6) is conducted at a temperature above 28 ℃ with aeration.
Preferably, the pine forest in the step (7) has a slope of more than 2 degrees, the sun-shading rate of the pine forest is 70-75%,
preferably, the KT-30 is forchlorfenuron (CPPU) and the NAA is naphthylacetic acid.
Preferably, the KT-30 is 0.5-1.8mg/L, the NAA is 6.1-8.5mg/L, and the prochloraz is 1.5-2.0 mg/L.
Compared with the prior art, the invention has the following advantages:
(1) the cutting mode of the invention adopts flat cutting, the ear making mode adopts flat cutting with the upper and lower surfaces being flat, the rooting solution adopts KT-30+ NAA + Bacterian combined hormone treatment solution, the substrate adopts river sand, the excellent asexual cutting propagation of the mother of the nux vomica has high survival rate, high rooting rate and good root system growth, the rooting rate and the survival rate both reach more than 93.8 percent, and the bottle seedling reaches 96.7 percent.
(2) The method has a simple cultivation mode, reduces the production cost of the excellent cutting propagation of the mother, and the mother of the mother is the mother of the mother, the mother meets the mother and the mother of the artificial mother of the artificial mother and the mother of the artificial mother of the artificial mother of the artificial mother.
(3) The method for asexual cutting propagation of Polygala crotalarioides has the advantages of simple operation, low cost, high survival rate and high rooting rate, and can be harvested after three to five years of Polygala crotalarioides cultivation generally, so that a high profit margin is obtained, and the method has important practical significance for developing forest economy, increasing the income of farmers and keeping rural stability.
Detailed Description
Example 1
Cutting branches growing in the same year from wild good mother plants of the wild mother and mother, which grow vigorously, are not damaged and do not have diseases and insect pests near four rows of mountains in Gunn county of Lincang city in Yunnan province, and respectively cutting the following 2 types of cutting slips: 1) cutting the stem with the top bud: selecting terminal bud segments of good branches of mother plants with terminal buds, cutting slips according to the standard of reserving 3-5 nodes and about 2cm in length, reserving terminal buds for each slip, cutting the lower incision into planes, and bundling 100 slips. 2) Cutting the shoot with the leaf stem section. Cutting the remaining branches of the Polygala crotalarioides with 2-3 nodes and about 2cm long again on the remaining branches of the Polygala crotalarioides with the apical bud stems, cutting the axillary buds 2mm away from the upper nodes of the cutting into a plane, cutting the lower cuts into a plane, cutting 100 bundles, and cutting the lower cuts into a plane with less roots, thereby being beneficial to the improvement of yield. In the process of making the cutting, the leaf surfaces are continuously sprayed with water to prevent the ear strips from losing water. Putting into a plastic bag filled with moss and taking back.
The cuttage of Polygala crotalarioides can be carried out in both spring and summer, and the optimum time is the local temperature of 22-27 ℃. Because the temperature is low in autumn and winter, the cell division is slow, the callus generation time is long, the cutting branches are easy to die, and the non-dead branches can root and sprout only after 3 months in the next year.
Before cuttage, sterilizing cuttage matrix with 3% potassium permanganate solution, filling into each cuttage bed (height 20cm, width 80cm, length 5m), leveling, watering thoroughly, and preparing cuttage propagation, wherein the cuttage matrix is river sand. Soaking the cutting shoots in a combined rooting solution KT-30(0.5mg/L) + NAA (6.1mg/L) + hymenide (1.5mg/L) for 25min until the liquid level of the soaking solution reaches 1-2cm height of the bottom of the cutting shoots.
After the cuttage is finished, the cuttage can be carried out, the row spacing of the cuttage is about 4cm, the plant spacing is about 4cm, every 100 cuttings are processed, a wood stick punching method is used, the cuttings are placed in holes, the cuttings are preferably 2/3 immersed in the soil, then the cuttings are compacted by hands, the base parts are in close contact with the matrix, then the water is poured, an arch-shaped support is placed on the surface of the cuttage bed, an agricultural plastic film is covered, and a sunshade net with the light transmittance of 90% is covered. If the conditions exist, an automatic spraying system is installed, and according to the weather conditions, a small amount of water drops on the leaf surfaces are sprayed into the cuttage bed to keep the relative humidity in the arched shed to be 80-95%. Under the condition of high temperature, when the temperature is higher than 28 ℃, proper ventilation is paid attention to, the rooting condition is frequently checked and recorded in daily management, weeding is carried out at any time, plant diseases and insect pests are prevented and removed in time, and the plants are rooted and germinated about 15 days after cuttage generally.
After the cutting seedlings survive, the cutting seedlings are planted in a wild-like mode and transplanted into artificial planting for planting, pine forests have certain slopes, the slopes are more than 2 degrees, the sun shading is 70-75%, the bed rising height along the slopes during planting is 20cm, the bed width is 1-1.2m, the planting row spacing is 20cm, and the branch spacing is 15cm, so that the cutting seedlings can absorb more nutrients and have larger growth space conveniently. The Polygala crotalarioides can be harvested after three to five years of cultivation. The implementation result shows that: the rooting rate is 95.7%, and the survival rate is 94.6%.
Example 2
Cutting branches growing in the same year from wild good mother plants of the wild mother and mother, which grow vigorously, are not damaged and do not have diseases and insect pests near four rows of mountains in Gunn county of Lincang city in Yunnan province, and respectively cutting the following 2 types of cutting slips: 1) cutting the stem with the top bud: selecting terminal bud segments of good branches of mother plants with terminal buds, cutting cuttings according to the standard of reserving 3-5 nodes and about 4cm long, reserving terminal buds for each cutting, cutting the lower cuts into planes, and cutting 100 cuttings. 2) Cutting the shoot with the leaf stem section. And (3) cutting the remaining branches of the Polygala crotalarioides with 2-3 nodes, about 4cm long, on the residual branches of the Polygala crotalarioides with the apical bud stems, cutting the axillary buds 4mm away from the upper nodes of the cutting into a plane, cutting the lower cuts into a plane, cutting 100 bundles, and cutting the lower cuts into a plane with less rooting, thereby being beneficial to the improvement of yield. In the process of making the cutting, the leaf surfaces are continuously sprayed with water to prevent the ear strips from losing water. Putting into a plastic bag filled with moss and taking back.
The cuttage of Polygala crotalarioides can be carried out in both spring and summer, and the local temperature is in the best period of 22-27 ℃. Because the temperature is low in autumn and winter, the cell division is slow, the callus generation time is long, the cutting branches are easy to die, and the non-dead branches can root and sprout only after 3 months in the next year.
Before cuttage, sterilizing cuttage matrix with 7% potassium permanganate solution, filling into each cuttage bed (height 20cm, width 80cm, length 5m), leveling, watering thoroughly, and preparing cuttage propagation, wherein the cuttage matrix is river sand. Soaking each cutting for 30min by using a combined rooting solution KT-30(1.8mg/L) + NAA (8.5mg/L) + hymenide (2.0mg/L), wherein the liquid level of the soaking solution reaches the height of 1-2cm at the bottom of the cutting when the soaking solution is soaked.
After the cuttage is finished, the cuttage can be carried out, the row spacing of the cuttage is about 7cm, the plant spacing is about 6cm, every 100 cuttings are processed, a wood stick punching method is used, the cuttings are placed in holes, the cuttings are preferably 2/3 immersed in the soil, then the cuttings are compacted by hands, the base parts are in close contact with the matrix, then the water is poured, an arch-shaped support is placed on the surface of the cuttage bed, an agricultural plastic film is covered, and a sunshade net with the light transmittance of 90% is covered. Spraying water into the cuttage bed for 1-3 times every day to maintain the relative humidity of the interior of the arch shed at 80-95%. Under the condition of high temperature, when the temperature is higher than 28 ℃, proper ventilation is paid attention to, the rooting condition is frequently checked and recorded in daily management, weeding is carried out at any time, plant diseases and insect pests are prevented and removed in time, and the plants are rooted and germinated about 15 days after cuttage generally.
After the cutting seedlings survive, the cutting seedlings are planted in a wild-like mode and transplanted into artificial planting for planting, pine forests have certain slopes, the slopes are more than 2 degrees, the sun shading is 70-75%, the bed rising height along the slopes during planting is 20cm, the bed width is 1-1.2m, the planting row spacing is 25cm, and the branch spacing is 20cm, so that the cutting seedlings can absorb more nutrients and have larger growth space conveniently. The Polygala crotalarioides can be harvested after three to five years of cultivation. The implementation result shows that: the rooting rate is 95.3%, and the survival rate is 95.2%.
Example 3
Cutting branches growing in the same year from wild good mother plants of the wild mother and mother, which grow vigorously, are not damaged and do not have diseases and insect pests near four rows of mountains in Gunn county of Lincang city in Yunnan province, and respectively cutting the following 2 types of cutting slips: 1) cutting the stem with the top bud: selecting terminal bud segments of good branches of mother plants with terminal buds, cutting cuttings according to the standard of reserving 3-5 nodes and about 3cm long, reserving terminal buds for each cutting, cutting the lower cuts into planes, and cutting 100 cuttings. 2) Cutting the shoot with the leaf stem section. Cutting the remaining branches of the Polygala crotalarioides with 2-3 nodes and about 3cm long again on the remaining branches of the Polygala crotalarioides with the apical bud stems, cutting the axillary buds 3mm away from the upper nodes of the cutting into planes, cutting the lower cuts into planes, cutting 100 bundles, and cutting the lower cuts into planes with less rooting, which is beneficial to the improvement of yield. In the process of making the cutting, the leaf surfaces are continuously sprayed with water to prevent the ear strips from losing water. Putting into a plastic bag filled with moss and taking back.
The cuttage of Polygala crotalarioides can be carried out in both spring and summer, and the optimum time is the local temperature of 22-27 ℃. Because the temperature is low in autumn and winter, the cell division is slow, the callus generation time is long, the cutting branches are easy to die, and the non-dead branches can root and sprout only after 3 months in the next year.
Sterilizing the cutting matrix with 5% potassium permanganate solution before cutting, filling into each cutting bed (height 20cm, width 80cm, length 5m), leveling, watering thoroughly, and preparing for cutting propagation, wherein the cutting matrix is river sand. Soaking each cutting for 30min by using a combined rooting solution KT-30(1.5mg/L) + NAA (8.1mg/L) + hymenide (1.7mg/L), wherein the liquid level of the soaking solution reaches the height of 1-2cm at the bottom of the cutting when the soaking solution is soaked.
After the cuttage is finished, the cuttage can be carried out, the row spacing of the cuttage is about 6cm, the plant spacing is about 5cm, every 100 cuttings are processed, a wood stick punching method is used, the cuttings are placed in holes, the cuttings are preferably 2/3 immersed in the soil, then the cuttings are compacted by hands, the base parts are in close contact with the matrix, then the water is poured, an arch-shaped support is placed on the surface of the cuttage bed, an agricultural plastic film is covered, and a sunshade net with the light transmittance of 90% is covered. Spraying water into the cuttage bed for 1-3 times every day to maintain the relative humidity of the interior of the arch shed at 80-95%. Under the condition of high temperature, when the temperature is higher than 28 ℃, proper ventilation is paid attention to, the rooting condition is frequently checked and recorded in daily management, weeding is carried out at any time, plant diseases and insect pests are prevented and removed in time, and the plants are rooted and germinated about 10 days after cuttage generally.
After the cutting seedlings survive, the cutting seedlings are planted in a wild-like mode and transplanted into artificial planting for planting, pine forests have certain slopes, the slopes are more than 2 degrees, the sun shading is 70-75%, the bed rising height along the slopes during planting is 20cm, the bed width is 1-1.2m, the planting row spacing is 22cm, and the branch spacing is 17cm, so that the cutting seedlings can absorb more nutrients and have larger growth space conveniently. The Polygala crotalarioides can be harvested after three to five years of cultivation. The implementation result shows that: the combined rooting solution is treated and soaked for 30min, the rooting rate is 96.7 percent, and the survival rate is 95.6 percent.
Claims (6)
1. The method for asexual cutting propagation of Polygala crotalarioides is characterized by comprising the following steps:
(1) collecting cutting slips
Cutting branches of the same year from a strong, nondestructive and disease and pest-free wild Polygala crotalarioides plant, and respectively cutting two cuttings of a stem section with a top bud and a stem section with leaves; the scion with the top bud stem section is the top bud section of a good branch with a top bud mother, the scion is cut according to a standard of reserving 3-5 nodes and 2-4cm in length, the top bud of each scion is reserved, and the lower incision is cut into a plane; cutting the cut with the leaf stem section into a plane by cutting 2-3 nodes and 2-4cm long on the residual branch of the Polygala crotalarioides with the cut with the top bud stem section, and cutting the axillary bud 2-4mm away from the upper node of the cut into a plane by cutting the lower cut into a plane;
(2) period of cuttage
The cuttage of Polygala crotalarioides can be carried out in both spring and summer;
(3) substrate preparation
Sterilizing a cutting matrix by using 3-7% potassium permanganate solution before cutting, filling the cutting matrix into a cutting bed, leveling, watering thoroughly, and preparing for cutting propagation, wherein the thickness of the matrix is 15-20 cm;
(4) preparation of cutting shoots
Soaking the bottoms of the cuttings for 25-35 minutes by using a rooting solution, wherein the liquid level of the soaking solution reaches the height of 1-2cm of the bottoms of the cuttings when the soaking solution is soaked, the rooting solution is KT-30+ NAA + Bacterite, the KT-30 is 0.5-1.8mg/L, the NAA is 6.1-8.5mg/L, and the Bacterite is 1.5-2.0 mg/L;
(5) cutting
After the cuttage is finished, cuttage can be carried out, the row spacing of the cuttage is 4-7cm, the plant spacing is 4-6cm, a wood stick punching method is used, the cutting slips are placed in holes, the cutting slips are preferably 2/3 immersed in the soil, then the cutting slips are compacted by hands, the bottoms of the cutting slips are in close contact with a cuttage matrix, then the water is poured, an arch-shaped support is placed on the surface of a cuttage bed, an agricultural plastic film is covered on the arch-shaped support, and then a sunshade net with the light transmittance of 90% is covered on the arch-shaped support;
(6) cultivation of
Spraying water into the cuttage bed for 1-3 times every day to keep the relative humidity in the arched shed at 80-95%, frequently checking and recording the rooting condition in daily management, weeding at any time, preventing and treating plant diseases and insect pests in time and removing diseased plants, and rooting and germinating 10-18 days after cuttage to become cuttage seedlings;
(7) transplanting
After the cutting seedling survives, planting in a wild-like manner, transplanting in an artificially planted pine forest, and planting with the bed height of 20cm, the bed width of 1-1.2m, the row spacing of 20-25cm and the branch spacing of 15-20 cm; the pine forest has a gradient of more than 2 degrees, and the sun-shading rate of the pine forest is 70-75%.
2. The method for vegetative cutting propagation of Polygala crotalarioides according to claim 1, wherein in the process of making cuttings in step (1), water is continuously sprayed on leaf surfaces to prevent water loss of cuttings.
3. The method for asexual cutting propagation of mother plant and plant branches according to claim 1, characterized in that the local air temperature in the cutting period of step (2) is 22-27 ℃.
4. The method for asexual cutting propagation of mother plant and plant of claim 1, wherein the substrate in step (3) is river sand.
5. The method for asexual cutting propagation of mother plant and plant of claim 1, wherein the cultivation period in step (6) is carried out at a temperature higher than 28 ℃ by ventilation.
6. The asexual cutting propagation method of mother plant and plant of claim 1, wherein the KT-30 is forchlorfenuron (CPPU) and the NAA is naphthylacetic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711427977.1A CN108142219B (en) | 2017-12-26 | 2017-12-26 | Fast asexual cuttage propagation method for Polygala crotalarioides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711427977.1A CN108142219B (en) | 2017-12-26 | 2017-12-26 | Fast asexual cuttage propagation method for Polygala crotalarioides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108142219A CN108142219A (en) | 2018-06-12 |
| CN108142219B true CN108142219B (en) | 2022-01-07 |
Family
ID=62462846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711427977.1A Active CN108142219B (en) | 2017-12-26 | 2017-12-26 | Fast asexual cuttage propagation method for Polygala crotalarioides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108142219B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667893A (en) * | 2022-03-25 | 2022-06-28 | 沧源奕岚佤医药开发有限公司 | Cultivation method of important Va drug resource Polygala crotalarioides |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711843A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for rooting |
| CN103553778A (en) * | 2013-10-25 | 2014-02-05 | 遵义师范学院 | Cephalotaxus fortune cutting seedling rooting-promoting compound conditioner and preparation method thereof |
| CN103999866A (en) * | 2014-04-28 | 2014-08-27 | 深圳市新华南方生物科技股份有限公司 | Rooting induction agent preparation method |
| CN105432316A (en) * | 2016-01-22 | 2016-03-30 | 岭南新科生态科技研究院（北京）有限公司 | Lycium barbarum cottage planting method used in desertification region |
-
2017
- 2017-12-26 CN CN201711427977.1A patent/CN108142219B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711843A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant growth regulating composition for rooting |
| CN103553778A (en) * | 2013-10-25 | 2014-02-05 | 遵义师范学院 | Cephalotaxus fortune cutting seedling rooting-promoting compound conditioner and preparation method thereof |
| CN103999866A (en) * | 2014-04-28 | 2014-08-27 | 深圳市新华南方生物科技股份有限公司 | Rooting induction agent preparation method |
| CN105432316A (en) * | 2016-01-22 | 2016-03-30 | 岭南新科生态科技研究院（北京）有限公司 | Lycium barbarum cottage planting method used in desertification region |
Non-Patent Citations (6)
| Title |
|---|
| 中药材工厂化快繁育苗技术;王敏珍等;《农业工程技术(温室园艺)》;20091010(第10期);第46-47页 * |
| 济南远志无公害栽培技术规程;王登良等;《山东农业科学》;20130930(第09期);第113-114页 * |
| 激素和基质对黄花远志扦插生根的影响试验;蔡仕珍等;《四川林业科技》;20050330(第01期);第65-67页 * |
| 蔡仕珍等.激素和基质对黄花远志扦插生根的影响试验.《四川林业科技》.2005,(第01期),第65-67页. * |
| 青岛崂山野生远志药材品质评价、驯化栽培及毛状根培养研究;潘俊;《中国优秀硕士学位论文全文数据库 农业科技辑》;20121231(第09期);第22-24、46-48页 * |
| 黄花倒水莲的开发利用价值与栽培管理技术;张冬生等;《农技服务》;20160320(第03期);第237-238页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108142219A (en) | 2018-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103141260B (en) | Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest | |
| CN111226704B (en) | Cutting seedling raising method for savatier shawense | |
| CN102246694B (en) | Tissue culture method of gynura divaricata | |
| CN103444530B (en) | Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian | |
| CN104094750A (en) | Cultivation method of green food cabbage mustard | |
| CN101461327A (en) | Rapid propagation technique of Paris polyphylla var. yunnanensis, seedling exercising and planting methods | |
| CN105284314A (en) | Cultivation method for passion fruit virus-free seedling | |
| CN106717742A (en) | A kind of greenhouse Chinese toon high-yield planting method | |
| CN111937703B (en) | Method for breeding high-quality polygonatum cyrtonema seedlings | |
| CN103004595A (en) | Twig cuttage breeding method for ginseng fruit | |
| CN103988672A (en) | Method for building shiny-leaved yellow horn tea garden and cultivating shiny-leaved yellow horn | |
| CN104663176A (en) | Planting method for honeysuckle | |
| CN107548921A (en) | The cultural method of caper in a kind of landscape greenhouse | |
| CN102144569B (en) | Method for rapidly propagating root and rhizome of Divaricate Gynura | |
| CN113115652A (en) | Cutting propagation method of yolk fruit | |
| CN113273464A (en) | Planting method and processing and storing method of ginseng/mountain ginseng/wild ginseng | |
| CN108142219B (en) | Fast asexual cuttage propagation method for Polygala crotalarioides | |
| CN107371695A (en) | A kind of cultural method of sponge gourd | |
| CN113170707B (en) | Efficient seedling growing method for mesona blume virus-free seedlings and application of efficient seedling growing method | |
| CN104604512A (en) | Cutting seedling method for zanthoxylum dissitum | |
| CN104054569A (en) | Selective breeding method production process of wild dendrobium officinale | |
| JP2018139593A (en) | Method of producing seedlings of glycyrrhiza plant | |
| CN114402821A (en) | Method for raising seedlings of semi-lignified twigs of agilawood by cutting | |
| JP6530584B2 (en) | Method of producing seedlings of licorice genus plant | |
| CN112425436A (en) | Planting method for intercropping blueberry and asparagus cochinchinensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |