CN108142219A - The female good asexual cuttage rapid propagating method of ma - Google Patents
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Abstract
The present invention relates to the asexual cuttage quick breeding technology fields of plant, and in particular to be the female good cuttage rapid propagating method of ma, include the following steps：(1) cuttings, the period of (2) cuttage are acquired, (3) matrix prepares, and (4) cuttings prepares, (5) cuttage, and (6) are cultivated, (7) transplanting.The cuttage mode of the present invention uses plug flat, fringe mode processed is using the truncation for being all up and down plane, solution of taking root uses the combination HORMONE TREATMENT liquid of KT 30+NAA+ Tuzets, matrix uses river sand, survival rate is high, rooting rate is high, root growth is good, and rooting rate and survival rate reach more than 93.8%, bottle seedling up to 96.7%.The female good vegetative propagation method of ma of the present invention is easy to operate, of low cost, survival rate is high, rooting rate is high, can harvest after general 3 to five years of the female good cultivation of ma, obtain rich profit rate, it increases farmers' income to development forest economy, steady countryside is kept to have important practical significance.
Description
Technical field
The present invention relates to the asexual cuttage quick breeding technology fields of plant, and in particular to be that the female good cuttage of ma is quickly numerous
Grow method.
Background technology
Female good system's transliteration (the Was's text of ma:Nya Miex Tiang), alias：Bifurcation mill ma, Nie Liang, are Polygalaceae rogation flower, are
Herbaceos perennial southwest Radix Polygalae (Polygala crotalarioides Buch.Ham.).Complete stool is used as medicine, especially with root effect
Most preferably, happiness is grown on the laterite of height above sea level 1300-2000m and faces south massif, dilute tree altoherbiprate and shrub-grass.Ah Was mountain area,
The ground such as Yun County, double rivers are distributed, and are a kind of civil rare Chinese herbal medicines of Yunnan Wa, and introducing medication history according to Wa herbalist has
More than 300 years, have improve a poor appetite, the work(that strengthening the muscles and bones, sexual function are hyperfunction and antifatigue, long-term use can make eyebrow elegant long, there is face
The benefits of facial expression pool and mole spot fading." bullfight contest ", earliest Wa are fed with bullfight with female good be used as " medicine of having an effect " of ma, make strength times
Increase, it is high in spirits.This product is warm-natured nontoxic, and taste is numb, peppery, fourth, and the thoughts of returning home, kidney, swollen warp have tranquilize the mind and promote the intelligence, eliminating the phlegm and detumescence, work
Blood and relieving pain, tonifying kidney and strengthening yang, antifatigue, anti-stress ability, male sex hormone sample and other effects.Pharmacological testing and clinical research show and
There are Immune-enhancing effect and immunologic adjuvant, antipsychotic, Adjust-blood lipid, anticoagulation, antithrombotic and a variety of physiological activity such as antitumor.It cures mainly
The hypogona disms such as breakdown of the normal physiological coordination between the heart and the kidney, kidney deficiency sun beach wormwood, premature ejaculation, kidney deficiency cough and asthma, wind-cold-dampness arthralgia, scrofula consumptive disease wound, rheumatic numbness, insomnia are more
The cards such as thin and fertility under dream, spit not well, senile dementia, forgetful palpitation with fear and mastosis, women's palace cold.
Main component isolated from this platymiscium is triterpene saponin componds, ketone, xanthone glycoside at present
Close object, oleanolic acid type saponin, oligosaccharides ester, alkaloid, flavonoids, Phenylpropanoid Glycosides class, cumarin and male sex hormone sample etc. and other
Organic principle and inorganic matter.According to Ping Fuliming torch Sun Xue favours《Wa's herbal medicine " bifurcation grinds ma " roborant pharmacological research》
Prove that it has the life of the testicualr development for being obviously promoted young age male mice and the sexual accessory gland organ of castration male rat atrophy
It is long, Tolerance of Swimming, oxygen deficit tolerance and anti-seismic design can be significantly increased." bifurcation grinds ma " can improve the anti-of body
Stress ability, and with Andrin-like action.
Since drug effect is distinguished, people fall over each other to excavate, and have resulted in the serious exhaustion of original producton location resource, oneself is on the verge of to disappear, and
Wild domestication survival rate is than relatively low, and therefore, to protect this rare herbal species, to ma, female good progress quick breeding technology is ground
It is important topic urgently to be resolved hurrily to study carefully.
At present, only domestic good research report female to ma focuses primarily upon two aspect of medicinal efficacy and medicinal ingredient, such as
" nya miex tiang film agent " disclosed in Chinese Patent Application No. 2009100945950, Chinese Patent Application No. 2009100945861 are public
" effervescing agent of nya miex tiang " opened, and it is cultivated, is bred and the correlative studys such as selection and breeding are less, particularly asexual Cutting test breeding
Technical research has not been reported.
Cuttage seeding is faster than seedling early growth, and breeding coefficient is high, at low cost, while can keep maternal Optimality
Shape, and seed collection is relatively difficult.In the case where experimental facilities and resource are limited, to ma, female good progress vegetative propagation is ground
Study carefully with innovative significance and realistic meaning.
Invention content
In view of the above-mentioned problems of the prior art, present invention aims at provide it is a kind of it is easy to operate, of low cost, into
The female good asexual cuttage rapid propagating method of ma that motility rate is high, rooting rate is high.
To achieve the above object, the present invention adopts the following technical scheme that：
The female good vegetative propagation method of ma, includes the following steps：
(1) cuttings is acquired
The branch of current year life is cut from robust growth, the female good plant of not damaged, no disease and pests harm wild ma, respectively clip band
Terminal bud stem section and two kinds of cuttings with base of leaf section；
(2) period of cuttage
The female good cuttage of ma can be carried out in spring and summer；
(3) matrix prepares
It is carried out disinfection before cuttage with the liquor potassic permanganate of 3-7% to cutting medium, cutting medium is then inserted into cuttage
Bed, stromal thickness 15-20cm, leveling sprinkle profoundly water, and prepare cutting propagation；
(4) cuttings prepares
With taking root, solution to each cuttings bottom impregnate 25-35 minutes, and the liquid level of soak reaches slotting during immersion
Fringe bottom 1-2cm high；
(5) cuttage
It can carry out cuttage after the completion of immersion, cuttage line-spacing 4-7cm, spacing in the rows 4-6cm, with waddy punch method, by cuttings
It is placed in hole, cuttings is advisable in not burying with 2/3, is then compacted with hand, makes cuttings bottom and cutting medium close contact, then
It sprinkles profoundly water, and arch support is placed on cuttage bed surface, overlying agricultural plastic film, then it is capped the sunshade that light transmittance is 90%
Net；
(6) it cultivates
Daily to cutting bed internal spraying moisture 1-3 times, relative humidity is 80-95% to keep in arched shed, in daily management
On, running check simultaneously records condition of rooting, weeding at any time, prevents pest and disease damage in time and pulls out diseased plant, 10-18 days after cuttage
It takes root and rudiment, becomes cuttage seeding；
(7) it transplants
After cuttage seeding survives, imitating wild planting is transplanted in the pine forests of artificial growth and transplants, along the hill start height of bed during plant
For 20cm, bedside 1-1.2m, line-spacing 20-25cm, branch diatance 15-20cm.
Preferably, the clip method of two kinds of cuttings in the step (1) with terminal bud stem section and with base of leaf section is：
1) band terminal bud stem section cuttings：Choose the terminal bud section of the female good branch of tool terminal bud ma, by retain 3-5 save, long 2-4cm
Standard clip cuttings, and each cuttings retains terminal bud, lower cut is cut into plane；
2) band base of leaf section cuttings：On the female good remaining branch of ma of clip terminal bud stem section, clip retains 2-3 again
The cuttings of section, long 2-4cm, and plane is cut into away from axillary bud 2-4mm notch at section in cuttings, lower cut is also cut into plane.
Preferably, it during the step (1) makes cuttings, constantly sprinkles water to blade face, prevents fringe bar dehydration.
Preferably, it is 22-27 DEG C that step (2) the cuttage period, which works as ground temperature,.
Preferably, the matrix of the step (3) is river sand.
Preferably, the solution of taking root of the step (4) is：KT-30+NAA+ Tuzets.
Preferably, the step (6) cultivates period when temperature is higher than 28 DEG C, is aerated processing.
Preferably, pine forests have 2 degree with upgrade in the step (7), and pine forests sunshade rate is 70-75%,
Preferably, the KT-30 is forchlorfenuron (CPPU), and the NAA is methyl α-naphthyl acetate.
Preferably, the KT-30 is 0.5-1.8mg/L, and the NAA is 6.1-8.5mg/L, and the Tuzet is 1.5-
2.0mg/L。
Compared with prior art, the present invention has the following advantages：
(1) cuttage mode of the invention is using plug flat, and fringe mode processed using the truncation for being all up and down plane, adopt by solution of taking root
With the combination HORMONE TREATMENT liquid of KT-30+NAA+ Tuzets, matrix uses river sand, and the female good vegetative propagation survival rate of ma is high, raw
Root rate is high, root growth is good, and rooting rate and survival rate reach more than 93.8%, bottle seedling up to 96.7%.
(2) type of rearing of the invention is simple, reduces the female good cutting propagation production cost of ma, meet commercially produce will
It asks.
(3) the female good vegetative propagation method of ma of the invention is easy to operate, of low cost, survival rate is high, rooting rate is high,
After general 3 to five years of the female good cultivation of ma can harvest, obtain rich profit rate, increase farmers' income, protect to development forest economy
Steady countryside is held to have important practical significance.
Specific embodiment
Embodiment 1
From near four row mountain of Geng Ma counties of Lincang City of Yunnan Province Natural Populations robust growth, not damaged, no disease and pests harm open country
Clip current year raw branch on the female good plant of raw ma, respectively 2 kinds of cuttings below clip：1) band terminal bud stem section cuttings：Choose tool top
The terminal bud section of the female good branch of bud ma, by the standard clip cuttings for retaining 3-5 section, long 2cm or so, and each cuttings retains top
Bud, lower cut are cut into plane, 100 piece of one bundle.2) band base of leaf section cuttings.In the female good remaining branch of the ma of clip terminal bud stem section
On, clip retains the cuttings of 2~3 sections, long 2cm or so again, and is cut into away from axillary bud 2mm at section in cuttings or so notch flat
Face, lower cut are also cut into plane, 100 piece of one bundle, and lower cut is cut into plane and takes root less, is conducive to the raising of yield,.It makes and inserts
During fringe, constantly sprinkle water to blade face, prevent fringe bar dehydration.It is put into be equipped in the fresh polybag of tongue and take back.
The female good cuttage of ma can be carried out in spring and summer, be best period when ground temperature is 22-27 DEG C.Because the autumn and winter day
Temperature is low, and cell division is slow, and the generation callus time is long, and cuttage skill item is easy to dead, and not dead also substantially will be in the coming year
March after can just take root rudiment.
It is carried out disinfection before cuttage with 3% liquor potassic permanganate to cutting medium, and inserts each cutting bed (high 20 cm, width
80cm, long 5m), stromal thickness 15cm, leveling sprinkles profoundly water, and prepares cutting propagation, cutting medium is river sand.With the life of combination
Root solution KT-30 (0.5mg/L)+NAA (6.1mg/L)+Tuzet (1.5mg/L) impregnates 25min to each cuttings, and when immersion impregnates
The liquid level of liquid reaches cuttings bottom 1-2cm high.
Cuttage can be carried out after the completion of immersion, cuttage line-spacing about 4cm, spacing in the rows about 4cm often handle 100 cuttings, use
Waddy punch method, cuttings is placed in hole, and cuttings is advisable in not burying with 2/3, is then compacted with hand, makes base portion close with matrix
Contact, then sprinkles profoundly water, and arch support is placed on cuttage bed surface, overlying agricultural plastic film, then be capped light transmittance and be
90% sunshade net.Automatic spraying system should be installed by such as having ready conditions, according to weather conditions, using blade face also have a small amount of droplets as degree to
Cutting bed internal spraying, to keep relative humidity 80-95% in arched shed.Under conditions of high temperature, when temperature is higher than 28 DEG C, pay attention to suitable
Work as ventilation, in daily management, running check simultaneously records condition of rooting, weeding at any time, prevents pest and disease damage in time and pulls out diseased plant,
It takes root within 15 days or so after general cuttage and rudiment.
After cuttage seeding survives, imitating wild planting is transplanted to the middle plant of artificial growth, and pine forests have certain slope, the gradient
It is 2 degree or more, sunshade is 70~75%, along the hill start height of bed is 20cm during plant, and bedside 1-1.2m, plant line-spacing is 20cm,
Branch diatance is 15cm, absorbs more nutrition and with bigger growing space in this way convenient for cuttage seeding.The female good cultivation general three of ma to
It can harvest after 5 years.Result of implementation shows：Rooting rate is 95.7%, survival rate 94.6%.
Embodiment 2
From near four row mountain of Geng Ma counties of Lincang City of Yunnan Province Natural Populations robust growth, not damaged, no disease and pests harm open country
Clip current year raw branch on the female good plant of raw ma, respectively 2 kinds of cuttings below clip：1) band terminal bud stem section cuttings：Choose tool top
The terminal bud section of the female good branch of bud ma, by the standard clip cuttings for retaining 3-5 section, long 4cm or so, and each cuttings retains top
Bud, lower cut are cut into plane, 100 piece of one bundle.2) band base of leaf section cuttings.In the female good remaining branch of the ma of clip terminal bud stem section
On, clip retains 2~3 sections, long, the cuttings of 4cm or so again, and is cut into away from axillary bud 4mm at section in cuttings or so notch flat
Face, lower cut are also cut into plane, 100 piece of one bundle, and lower cut is cut into plane and takes root less, is conducive to the raising of yield,.It makes and inserts
During fringe, constantly sprinkle water to blade face, prevent fringe bar dehydration.It is put into be equipped in the fresh polybag of tongue and take back.
The female good cuttage of ma can be carried out in spring and summer, when ground temperature is 22-27 DEG C of best period.Because autumn and winter weather
Temperature is low, and cell division is slow, and the generation callus time is long, and cuttage skill item is easy to dead, and not dead also substantially will be the 3 of the coming year
Can just take root rudiment after month.
It is carried out disinfection before cuttage with 7% liquor potassic permanganate to cutting medium, and inserts each cutting bed (high 20 cm, width
80cm, long 5m), stromal thickness 20cm, leveling sprinkles profoundly water, and prepares cutting propagation, cutting medium is river sand.With the life of combination
Root solution KT-30 (1.8mg/L)+NAA (8.5mg/L)+Tuzet (2.0mg/L) impregnates 30min to each cuttings, and when immersion impregnates
The liquid level of liquid reaches cuttings bottom 1-2cm high.
Cuttage can be carried out after the completion of immersion, cuttage line-spacing about 7cm, spacing in the rows about 6cm often handle 100 cuttings, use
Waddy punch method, cuttings is placed in hole, and cuttings is advisable in not burying with 2/3, is then compacted with hand, makes base portion close with matrix
Contact, then sprinkles profoundly water, and arch support is placed on cuttage bed surface, overlying agricultural plastic film, then be capped light transmittance and be
90% sunshade net.Daily to cutting bed internal spraying moisture 1-3 times, to keep relative humidity 80-95% in arched shed.In high temperature
Under conditions of, when temperature is higher than 28 DEG C, pay attention to suitably divulging information, in daily management, running check simultaneously records condition of rooting, at any time
Weeding prevents pest and disease damage and simultaneously pulls out diseased plant in time, takes root and rudiment within 15 days or so after general cuttage.
After cuttage seeding survives, imitating wild planting is transplanted to the middle plant of artificial growth, and pine forests have certain slope, the gradient
It is 2 degree or more, sunshade is 70~75%, along the hill start height of bed is 20cm during plant, and bedside 1-1.2m, plant line-spacing is 25cm,
Branch diatance is 20cm, absorbs more nutrition and with bigger growing space in this way convenient for cuttage seeding.The female good cultivation general three of ma to
It can harvest after 5 years.Result of implementation shows：Rooting rate is 95.3%, survival rate 95.2%.
Embodiment 3
From near four row mountain of Geng Ma counties of Lincang City of Yunnan Province Natural Populations robust growth, not damaged, no disease and pests harm open country
Clip current year raw branch on the female good plant of raw ma, respectively 2 kinds of cuttings below clip：1) band terminal bud stem section cuttings：Choose tool top
The terminal bud section of the female good branch of bud ma, by the standard clip cuttings for retaining 3-5 section, long 3cm or so, and each cuttings retains top
Bud, lower cut are cut into plane, 100 piece of one bundle.2) band base of leaf section cuttings.In the female good remaining branch of the ma of clip terminal bud stem section
On, clip retains the cuttings of 2~3 sections, long 3cm or so again, and is cut into away from axillary bud 3mm at section in cuttings or so notch flat
Face, lower cut are also cut into plane, 100 piece of one bundle, and lower cut is cut into plane and takes root less, is conducive to the raising of yield,.It makes and inserts
During fringe, constantly sprinkle water to blade face, prevent fringe bar dehydration.It is put into be equipped in the fresh polybag of tongue and take back.
The female good cuttage of ma can be carried out in spring and summer, be best period when ground temperature is 22-27 DEG C.Because the autumn and winter day
Temperature is low, and cell division is slow, and the generation callus time is long, and cuttage skill item is easy to dead, and not dead also substantially will be in the coming year
March after can just take root rudiment.
It is carried out disinfection before cuttage with 5% liquor potassic permanganate to cutting medium, and inserts each cutting bed (high 20 cm, width
80cm, long 5m), stromal thickness 18cm, leveling sprinkles profoundly water, and prepares cutting propagation, cutting medium is river sand.With the life of combination
Root solution KT-30 (1.5mg/L)+NAA (8.1mg/L)+Tuzet (1.7mg/L) impregnates 30min to each cuttings, and when immersion impregnates
The liquid level of liquid reaches cuttings bottom 1-2cm high.
Cuttage can be carried out after the completion of immersion, cuttage line-spacing about 6cm, spacing in the rows about 5cm often handle 100 cuttings, use
Waddy punch method, cuttings is placed in hole, and cuttings is advisable in not burying with 2/3, is then compacted with hand, makes base portion close with matrix
Contact, then sprinkles profoundly water, and arch support is placed on cuttage bed surface, overlying agricultural plastic film, then be capped light transmittance and be
90% sunshade net.Daily to cutting bed internal spraying moisture 1-3 times, to keep relative humidity 80-95% in arched shed.In high temperature
Under conditions of, when temperature is higher than 28 DEG C, pay attention to suitably divulging information, in daily management, running check simultaneously records condition of rooting, at any time
Weeding prevents pest and disease damage and simultaneously pulls out diseased plant in time, takes root and rudiment within 10 days or so after general cuttage.
After cuttage seeding survives, imitating wild planting is transplanted to the middle plant of artificial growth, and pine forests have certain slope, the gradient
It is 2 degree or more, sunshade is 70~75%, along the hill start height of bed is 20cm during plant, and bedside 1-1.2m, plant line-spacing is 22cm,
Branch diatance is 17cm, absorbs more nutrition and with bigger growing space in this way convenient for cuttage seeding.The female good cultivation general three of ma to
It can harvest after 5 years.Result of implementation shows：It combines solution treatment of taking root and impregnates 30min, rooting rate 96.7%, survival rate is
95.6%.
Claims (10)
1. the female good vegetative propagation method of ma, which is characterized in that include the following steps：
(1) cuttings is acquired
The branch of current year life is cut from robust growth, the female good plant of not damaged, no disease and pests harm wild ma, respectively clip band terminal bud
Stem section and two kinds of cuttings with base of leaf section；
(2) period of cuttage
The female good cuttage of ma can be carried out in spring and summer；
(3) matrix prepares
It is carried out disinfection before cuttage with the liquor potassic permanganate of 3-7% to cutting medium, cutting medium is then inserted into cutting bed, base
Matter thickness is 15-20cm, and leveling sprinkles profoundly water, and prepares cutting propagation；
(4) cuttings prepares
With taking root, solution to each cuttings bottom impregnate 25-35 minutes, and the liquid level of soak reaches cuttings bottom during immersion
Portion 1-2cm high；
(5) cuttage
It can carry out cuttage after the completion of immersion, with waddy punch method, cuttings is placed in by cuttage line-spacing 4-7cm, spacing in the rows 4-6cm
In hole, cuttings is advisable in not burying with 2/3, is then compacted with hand, makes cuttings bottom and cutting medium close contact, then irrigates
Water, and arch support is placed on cuttage bed surface, overlying agricultural plastic film, then it is capped the sunshade net that light transmittance is 90%；
(6) it cultivates
Daily to cutting bed internal spraying moisture 1-3 times, relative humidity is 80-95% to keep in arched shed, in daily management, warp
It often checks and records condition of rooting, weeding at any time prevents pest and disease damage and pull out diseased plant, can take root within 10-18 days after cuttage in time
And rudiment, become cuttage seeding；
(7) it transplants
After cuttage seeding survives, imitating wild planting is transplanted in the pine forests of artificial growth and transplants, and is along the hill start height of bed during plant
20cm, bedside 1-1.2m, line-spacing 20-25cm, branch diatance 15-20cm.
2. the female good vegetative propagation method of ma according to claim 1, which is characterized in that band top in the step (1)
The clip method of leaf stem section and two kinds of cuttings with base of leaf section is：
1) band terminal bud stem section cuttings：The terminal bud section of the female good branch of tool terminal bud ma is chosen, by the standard for retaining 3-5 section, long 2-4cm
Clip cuttings, and each cuttings retains terminal bud, lower cut is cut into plane；
2) band base of leaf section cuttings：On the female good remaining branch of ma of clip terminal bud stem section, clip retains 2-3 section, length again
The cuttings of 2-4cm, and plane is cut into away from axillary bud 2-4mm notch at section in cuttings, lower cut is also cut into plane.
3. the female good vegetative propagation method of ma according to claim 1 or 2, which is characterized in that the step (1) makes
During cuttings, constantly sprinkle water to blade face, prevent fringe bar dehydration.
4. the female good vegetative propagation method of ma according to claim 1, which is characterized in that during step (2) cuttage
Phase is 22-27 DEG C when ground temperature.
5. the female good vegetative propagation method of ma according to claim 1, which is characterized in that the matrix of the step (3)
For river sand.
6. the female good vegetative propagation method of ma according to claim 1, which is characterized in that step (4) take root
Solution is：KT-30+NAA+ Tuzets.
7. the female good vegetative propagation method of ma according to claim 1, which is characterized in that when the step (6) is cultivated
Phase when temperature is higher than 28 DEG C, is aerated processing.
8. the female good vegetative propagation method of ma according to claim 1, which is characterized in that pine tree in the step (7)
Woods has 2 degree with upgrade, and pine forests sunshade rate is 70-75%.
9. the female good vegetative propagation method of ma according to claim 6, which is characterized in that the KT-30 is chlorine pyrrole benzene
Urea (CPPU), the NAA are methyl α-naphthyl acetate.
10. the female good vegetative propagation method of ma according to claim 6, which is characterized in that the KT-30 is 0.5-
1.8mg/L, the NAA are 6.1-8.5mg/L, and the Tuzet is 1.5-2.0mg/L.
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| CN201711427977.1A CN108142219B (en) | 2017-12-26 | 2017-12-26 | Fast asexual cuttage propagation method for Polygala crotalarioides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667893A (en) * | 2022-03-25 | 2022-06-28 | 沧源奕岚佤医药开发有限公司 | Cultivation method of important Va drug resource Polygala crotalarioides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667893A (en) * | 2022-03-25 | 2022-06-28 | 沧源奕岚佤医药开发有限公司 | Cultivation method of important Va drug resource Polygala crotalarioides |
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