CN108094325A - A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy - Google Patents

A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy Download PDF

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Publication number
CN108094325A
CN108094325A CN201810092990.4A CN201810092990A CN108094325A CN 108094325 A CN108094325 A CN 108094325A CN 201810092990 A CN201810092990 A CN 201810092990A CN 108094325 A CN108094325 A CN 108094325A
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weight
parts
chicken
spice
longevity perfume
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唐仲举
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Guangxi Bama Eco Agriculture Development Co Ltd
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Guangxi Bama Eco Agriculture Development Co Ltd
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Priority to CN201810092990.4A priority Critical patent/CN108094325A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/22—Compounds of alkali metals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to cultural technique fields, and in particular to a kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, including step (1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possess the solarization time reach 68 it is small when hillside as farm；(2) henhouse construction；(3) hatch；(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；(5) health care cultivation is handled；(6) deliver for sale.The longevity perfume (or spice) chicken yield of the cultivation of the present invention out reach 2.8 3.5 kilograms/only, deliver listing rate for sale and reach more than 95%, vitamin, protein, sugared content ratio on be improved, quality gets a promotion, and economic value content is high.

Description

A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy
【Technical field】
The invention belongs to cultural technique fields, and in particular to a kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy.
【Background technology】
Longevity perfume (or spice) chicken then enjoys the salubrious sweetness of the natural mountain spring water of bar horse, and seat gathers around 5000 mu of natural forest farms freely, enjoys gloomy 220 days natural growth cycles are spent in the favor of woods natural equilibrium food chain, perfection, are qualified " longevity township of world sylvogenic soil Chicken " is known as " Gu member increases first of longevity chicken ", is to make poultry geographical sign star's product a bar horse future.
With the transition in epoch, requirement of the people to food is higher and higher, for the progress kept pace with the times, promotes longevity perfume (or spice) chicken Sales volume do not reduce, improve longevity perfume (or spice) chicken quality breeding way will be it is a kind of it is better improve longevity perfume (or spice) chicken economic value side Method.
【The content of the invention】
The object of the present invention is to provide a kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, cultivation of the invention comes out Longevity perfume (or spice) chicken yield reach 2.8-3.5 kilograms/only, deliver listing rate for sale and reach more than 95%, in vitamin, protein, sugared content Ratio on be improved, quality gets a promotion, and economic value content is high.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses The solarization time reach 6-8 it is small when hillside as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more The solarization time reach 6-8 it is small when, 80-100 square metres of the area of every henhouse, henhouse spacing reach 800 meters or more, every is put in a suitable place to breed Longevity perfume (or spice) chicken density for 1000-1500 only,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and taken out, continued to hatch to longevity perfume (or spice) chicken hen, incubate Longevity change phase perfume (or spice) egg is to be positioned over nest on the rice straw after disinfection with 4-6, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated It is 0.2-0.5m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed, The first time free-ranging since the 3rd day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out Column, the time into column, and send alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream Dynamic mountain spring water, the 3-4 after fed with water start when small to the first mixed fodder of second month feeding, injected volume for 100-200g/ only； Second month started the second mixed fodder of feeding to four month, and injected volume is 200-300g/, when longevity perfume (or spice) chicken was at four month To seven month the 3rd mixed fodder of feeding, injected volume was 200-300g/；
First mixed fodder is mixed to get by following raw material：Soy meal 20-40 parts by weight, corn flour 30-50 weight Part, chicken rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium acid carbonate 0.3-0.8 parts by weight, Fiery sesame oil 0.1-0.6 parts by weight；
Second mixed fodder is prepared by following raw material：Soy meal 15-30 parts by weight, corn flour 10-20 weight Part, chicken rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium acid carbonate 0.5-1.0 parts by weight, fire fiber crops Oily 0.2-0.6 parts by weight, pumpkin peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, locust 3-10 parts by weight；
3rd mixed fodder is prepared by following raw material：Dregs of beans 10-25 parts by weight, wheat bran 10-20 parts by weight, fish Powder 10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium acid carbonate 0.3-0.8 parts by weight, yellow meal worm 5- 10 parts by weight, fermentation material 5-15 parts by weight, diseases prevention liquid 0.5-5.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is Only, the health care addition feed is prepared 25-50g/ by following raw material：Dendrobium candidum 10-20 parts by weight, bamboo shoot 1-3 weights Measure part, sweet osmanthus 0.5-1.2 parts by weight, mulberry leaf powder 0.5-1.0 parts by weight；
(6) deliver for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
It further illustrates in the present invention, the fermentation material is prepared by following raw material：Sweet tea napier grass 15-25 parts by weight, Green bristlegrass 10-15 parts by weight, sericin fixation 10-15 parts by weight, extract of soybean 15-25 parts by weight, mixing beneficial bacterium 3-6 weight Part.
It further illustrates in the present invention, the mixing beneficial bacterium is mixed to get by following raw material：Cellulase 3-8 weight Part, Brevibacillus 2-6 parts by weight, saccharomyces pastorianus bacterium 2-4 parts by weight, bifidobacterium bifidum 2-5 parts by weight.
It further illustrates in the present invention, the viable count of the cellulase is hundred million cfu/g of 3-5, Brevibacillus Viable count is hundred million cfu/g of 3-8, the viable count of saccharomyces pastorianus bacterium is hundred million cfu/g of 2-5, the viable count of bifidobacterium bifidum is 3-8 Hundred million cfu/g.
It further illustrates in the present invention, the fermentation material is prepared by following methods：
The water that good soybean is selected to add in 3-6 times of volume is blended 10-15 minutes loading conical flasks of mashing by the first step In, the sodium bicarbonate solution adjusting pH to 5-6 for being subsequently added into 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2- The mixing protease of 3% volume carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieves, use Centrifuge carries out centrifugation and can obtain extract of soybean in 2-5 minutes；
Second step adds in sealing high steam pot, then passes to 300-500 DEG C after fresh sericin fixation is cleaned up Steam carry out boiling 5-10 minute, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition dries It does to moisture content 15-20%；
3rd step with the material obtained after the processing of first and second step after crushing green bristlegrass, sweet tea napier grass, is subsequently added into mixing Beneficial bacterium is carried out after building heap fermentation 5-8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
It further illustrates in the present invention, the mixing protease is by 2-6 parts by weight ficin, 2-5 weight Part subtilopeptidase A, 1-5 parts by weight papain, 1-3 parts by weight bromelain composition.
It further illustrates in the present invention, the rate of the shaking table is 80-100r/min, the centrifugal rotational speed of the centrifuge For 2000-2500r/min.
It further illustrates in the present invention, the diseases prevention liquid is prepared by following raw material：Thalictrum extract 10- 20mg/l, kuh-seng seed extract 20-30mg/l, oriental cherry bark extract 40-80mg/l, extractive of pubescent angelica root coumarin 50-70mg/l, crow Imperial tea extraction 60-90mg/l, forsythia suspense extraction 60-100mg/l, Flos Chrysanthemi Indici extract 50-70mg/l, Fructus Piperis extract 50-70mg/l。
It further illustrates in the present invention, the Thalictrum extract is prepared by following methods：By grass of meadow rue It cleans and crushes, add in 50-65% (v/v) ethanol solutions and heating and refluxing extraction is carried out at 50-60 DEG C 10-20 minutes, take extraction Liquid is concentrated under reduced pressure up to no ethanol flavor, and the acetum for being subsequently added into 5-10 times of volume adjusts pH to 4.5-5.5, at 55 DEG C, 100-120 hertz of high-voltage pulse electric field pulse processing 2 × 10-6-3×10-6S extracts 20-30 minutes, obtains crude extract, then Layering extraction is carried out using ethyl acetate, ethyl acetate portion is taken to be concentrated under reduced pressure, spray drying can obtain Thalictrum extract, carry Master in object is taken to contain alkaloid 48.21-64.22mg/g；
The kuh-seng seed extract is prepared by following methods：Suitable silicon is added in after kuh-seng seed is cleaned Diatomaceous earth carries out mortar grinder into slurry, is subsequently added into 50-65% (v/v) ethanol solutions and heating and refluxing extraction is carried out at 40-50 DEG C 5-10 minutes, filtrate is filtered to take, filtrate is subsequently added into dilute hydrochloric acid and adjusts pH by the recycling ethyl alcohol that is concentrated under reduced pressure up to no alcohol taste Supernatant is filtered to take after to 4.0-5.0, cellulase is subsequently added into water enzyme digestion 20-30 minutes at 45-50 DEG C, is subsequently added into Sodium hydroxide adjusts pH to neutrality, filters to take precipitation and can obtain kuh-seng seed extract, master contains total alkaloid in extract
46.35-51.35mg/g。
It further illustrates in the present invention, the oriental cherry bark extract is prepared by following methods：By oriental cherry Bark crosses 10-20 mesh sieves after ultramicro grinding is carried out under pressure 3-5MPa, adds in 10-20 times of water refluxing extraction at 50-60 DEG C Extraction obtains extracting solution in 20-30 minutes, and the petroleum ether for then adding in 1.2-1.5 times of extracting liquid volume is extracted, and takes petroleum ether Portion then recycles petroleum ether with water-bath distillation, be subsequently added into 80-85% (v/v) ethanol solution carry out alcohol extracting 1-2 it is small when, then use Macroreticular resin carry out adsorbing separation elution, elution successively with volumetric concentration be 20%, 40%, 60%, 80% and 90% ethyl alcohol- Aqueous systems eluent carries out gradient elution, and the ethanol-water solution elution volume of each gradient is 5-6BV, elution flow rate 3- 4BV/h is concentrated under reduced pressure to give paste, and dry to can obtain flowering cherry bark extract, master contains coumaric acid glycosides in extract 64.25-78.15mg/g；
The extractive of pubescent angelica root coumarin is prepared by following methods：White oil, diatomite grinding are added in after Radix Angelicae Pubescentis is cleaned Into slurry, then add in 80-85% (v/v) ethanol solutions refluxing extraction 3 times, the temperature extracted every time is 60-70 DEG C, extraction when Between for 30-40 minute, merge the extracting solutions of 3 extractions, after adding in activated carbon decolorizing filtering remove activated carbon, be then diluted with water Afterwards add in n-butanol vibration rock after stand 5-10 minutes, then n-butanol fraction is taken to be separated with silica gel column chromatography, with petroleum ether- Ethyl acetate carries out gradient elution for eluant, eluent, 40：1 elution fraction carries out 5 times with acetone and is recrystallized to give extractive of pubescent angelica root coumarin It is main to contain angelica lactone 19.35-27.54mg/g；The Radix Angelicae Pubescentis：White oil：Diatomaceous weight ratio is 2-3:0.05-0.07： 0.1-0.2。
It further illustrates in the present invention, the oolong tea extract is prepared by following methods：By oolong tea It is dry to cross 50-100 mesh sieves after progress ultramicro grinding under pressure 3-5MPa, the use of 3-5 times of volume, temperature is 100-120 DEG C Water termostat impregnates oolong tea tea powder and obtains soup tea in 5-10 minutes, is subsequently added into vinegar acid for adjusting pH to 2.0-3.0, uses 65-75% (v/v) ethanol solution refluxing extraction takes extracting solution, is 1 then according to volume ratio:1 ratio adds in ethyl acetate and is swayed Extraction is stood after 20-30 minutes, ethyl acetate portion is separated with silica gel column chromatography, using petroleum ether-ethyl acetate as eluant, eluent Gradient elution is carried out, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give oolong tea extract master containing tea polyphenols 304.15-322.18mg/g；
The forsythia suspense extraction is prepared by following methods：After Fructus Forsythiae is crushed, 80-85% (v/v) second is added in Alcoholic solution carries out refluxing extraction 30-40 minutes under ultrasonic wave subsidiary conditions at 80-90 DEG C, and refluxing extraction 3 times, is closed altogether And No. 3 extracting solutions are concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can are obtained forsythia suspense extraction master and contained There is epicatechin 22.34-28.64mg/g.
It further illustrates in the present invention, the Flos Chrysanthemi Indici extract is prepared by following methods：By wild chrysanthemum It adds in 10-20 times of water to decoct 50-60 minutes, filters to take filtrate, reduction vaporization, which is concentrated into 1.02-1.05 times of original volume, to be obtained To the Flos Chrysanthemi Indici extract of wild chrysanthemum active ingredient；
Fructus Piperis extract is prepared by following methods：White pepper is added in 10-20 times of water and decocts 50-60 points Clock, filters to take filtrate, is evaporated under reduced pressure to be concentrated into 1.02-1.05 times of original volume and can obtain the white pepper of white pepper active ingredient and carries Take object.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. being cultivated in the application using original free-ranging mode, longevity perfume (or spice) chicken freely lives with enough during being cultivated Dynamic time and space, can freely prey on, freely run so that the muscle of longevity perfume (or spice) chicken is sturdy, without fishy smell, chicken fat content is few, taste Delicious and edible water is pollution-free containing albic material is few, Mineral Elements Content is high for the mountain spring water of flowing, can improve longevity perfume (or spice) chicken Digestive system ability；By freely preying on, free of contamination mountain spring water living is eaten, reaches the cultivation of ecosystem, but also deposits It is not high in the longevity perfume (or spice) chicken yield of original ecological breeding, because easily infectious disease, delivers for sale in the epoch of this progress in order to reach raising Yield, many farms are cultivated using the mode of stable breeding, not only output increased, and marketing time also shortens, to reach To the economic benefit for improving businessman, but with the variation in epoch, people increasingly pursues quality, and therefore, this has been taken a fancy to by my company The business opportunity of sample is found in continuous research, with addition of the mode of stable breeding and training feeding in the mode of free-ranging, can not only be improved The yield delivered for sale, the longevity perfume (or spice) chicken delivered for sale also have very high health-care effect, substantially increase the quality of longevity perfume (or spice) chicken, and then greatly improve The economic benefit of longevity perfume (or spice) chicken.
2. using different feeds is launched stage by stage, contain abundant protein, amino acid, dimension life in the first mixed fodder Element, with addition of fiery sesame oil can supplement linoleic acid content promote chicken growth, and then can fully absorb the protein in feed, Amino acid, vitamin, and then the utilization rate of feed is improved, and the feed intake of chicken can be improved, achieve the purpose that not waste feed, So that feed is reasonably utilized, the conversion ratio of feed is improved.In second mixed fodder, the ratio of the formula of feed is had adjusted Example, with addition of pumpkin peel after digestive system there is removing toxic substances, promote to digest, and with the nutrition such as starch enriched into Point, hayashishita weeds, which provide cellulose, can enhance digestion, and locust is locust living, can improve the grazing rate of chicken, and provides albumin For the demand of its growth, with addition of feed in achieved the purpose that make full use of agricultural waste material；It is each in 3rd mixed fodder Each performs its own functions for a raw material, and complements each other, and the nutritional ingredient provided adapts to use in the longevity perfume (or spice) chicken feed of growth 4-6 months, and nutrition is complete Face is balanced, is capable of providing longevity perfume (or spice) chicken nutrient elements required for the growth, without slow-growing because nutrient is lacked, and then It ensure that the marketing time of longevity perfume (or spice) chicken.
It is that a kind of one kind of energy utilization of resources weeds is new 3. in the fermentation material of addition harmful weeds make use of to turn waste into wealth Processing mode, but sericin fixation, which can digest to generate after hydrogen cyanide longevity perfume (or spice) chicken feed is used in being fermented, can cause brain, angiocarpy Etc. systems dysfunction, therefore be unable to eat the cyaniding for needing further to carry out processing degradation sericin fixation after direct fermentation Object, the applicant by noxious material by that the study found that by the technological treatment of the application, rapidly can carry out detoxification drop The toxicity of itself has been solved, has reduced the amount in fermentation release, and during the fermentation due to there is temperature to have what microbial action generated Chemical reaction can cause a series of change of properties, generate some sorbefacient nutriments and promote growth of animal because Son, so far not yet there are one detailed report principle therein, therefore it is that have very much must that detoxification is carried out before fermentation to raw material It wants, is found after detoxification by research so that noxious material burst size is reduced but not thoroughly in fermentation process, by multiple The microorganism matched somebody with somebody carries out the content that degradation substantially reduces noxious material, adds the security of feed, and finds noxious material drop The content of organics of feed increases after solution.
4. 8 kinds of extracts are respectively different active site extraction units in the diseases prevention liquid of the application, Each performs its own functions, Xiang Fuxiang Into synergistic effect plays the purpose of prevention longevity perfume (or spice) chicken coccidiasis, by containing life in the substance in the raw extract of the application Alkaloids, phenols, tannin class, phenolic acid class, glycoside, are tested by the applicant and matched, and the active ingredient of each raw material complements each other Reach synergistic afterwards and the detoxication of the toxicity of alkaline matter can be released, more than 98% prevention longevity perfume is reached after compounding The effect of morbidity and the poisoning of chicken coccidiasis reduces the disease of global-worm illness, and so as to reduce the disease rate of longevity perfume (or spice) chicken, increase is delivered for sale Yield.
5. adding feed for feeding health care in 3 months before delivering for sale in the application, health care addition feed is by fresh iron sheet stone Active polysaccharide extraction unit, sweet osmanthus active polysaccharide extraction unit with addition of bamboo shoot after dry measure used in former times is beaten obtains after mulberry leaf powder mixing, The health care adds feed longevity perfume (or spice) chicken feed with after 3 months, applicants have discovered that, the longevity perfume (or spice) chicken delivered for sale carries after culinary art in fresh meat The fragrant of dendrobium candidum, the chicken nugget of the longevity perfume (or spice) chicken to delivering for sale carry out the detection discovery of nutrient composition content, compare do not going out The longevity perfume (or spice) chicken of health care addition feed is fed within 3 months before column, amino acid content improves 2.52-3.73%, and stem of noble dendrobium alkali content carries High 0.54-1.25%, protein content improve 5.36-8.14%, and sugared content improves 0.05-0.12%.
【Specific embodiment】
Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with Much implement different from other manner described here, those skilled in the art can be in the situation without prejudice to intension of the present invention Under do similar improvement, therefore the present invention is not limited to the specific embodiments disclosed below.
Embodiment 1：
A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses The solarization time reach 6 it is small when hillside as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more The solarization time reach 6 it is small when, 80 square metres of the area of every henhouse, henhouse spacing reaches 800 meters or more, and every longevity for putting in a suitable place to breed is fragrant Chicken density is 1000,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched Longevity phase perfume (or spice) egg is to be positioned over nest on the rice straw after disinfection with 4, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period Between keep the coop ventilation amount to be 0.2m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed, The first time free-ranging since the 3rd day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out Column, the time into column, and send alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream Dynamic mountain spring water, 3 after fed with water start when small to the first mixed fodder of second month feeding, injected volume for 100g/ only；Second The moon started the second mixed fodder of feeding to four month, and injected volume is 200g/, when longevity perfume (or spice) chicken was at four month to seven month The 3rd mixed fodder of feeding, injected volume are 200g/；
First mixed fodder is mixed to get by following raw material：20 parts by weight of soy meal, 30 parts by weight of corn flour, chicken intestines 10 parts by weight of powder, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium acid carbonate, 0.1 parts by weight of fiery sesame oil；
Second mixed fodder is prepared by following raw material：15 parts by weight of soy meal, 10 parts by weight of corn flour, chicken intestines 10 parts by weight of powder, 3 parts by weight of amino acid, 1 parts by weight of salt, 0.5 parts by weight of sodium acid carbonate, 0.2 parts by weight of fiery sesame oil, pumpkin peel 5 parts by weight, 5 parts by weight of hayashishita weeds, 3 parts by weight of locust；
3rd mixed fodder is prepared by following raw material：10 parts by weight of dregs of beans, 10 parts by weight of wheat bran, 10 weight of fish meal Measure part, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium acid carbonate, 5 parts by weight of yellow meal worm, 5 parts by weight of fermentation material, Diseases prevention liquid 0.5g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is Only, the health care addition feed is prepared 25g/ by following raw material：10 parts by weight of dendrobium candidum, 1 parts by weight of bamboo shoot, sweet osmanthus 0.5-1.2 parts by weight, 0.5 parts by weight of mulberry leaf powder；
(6) deliver for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned 3rd mixed fodder：15 parts by weight of sweet tea napier grass, dog 10 parts by weight of tail grass, 10 parts by weight of sericin fixation, 15 parts by weight of extract of soybean, mixing beneficial bacterium 3-6 parts by weight are (by following original Material is mixed to get：3 parts by weight of cellulase that viable count is 300,000,000 cfu/g, the Brevibacillus 2 that viable count is 300,000,000 cfu/g 2 parts by weight of saccharomyces pastorianus bacterium that parts by weight, viable count are 200,000,000 cfu/g, the bifidobacterium bifidum 2 that viable count is 300,000,000 cfu/g Parts by weight)；
One of raw material fermentation material is prepared by following methods in above-mentioned 3rd mixed fodder：
The water for selecting 3 times of volumes of good soybean addition is blended mashing and is fitted into conical flask for 10 minutes, then by the first step Add in 0.05% (w/v) sodium bicarbonate solution adjust pH to 5 obtain mixed serum, add 2% volume of slurries by 2 weight Part ficin, 2 parts by weight subtilopeptidase As, 1 parts by weight papain, 1 parts by weight bromelain composition Mixing protease in conical flask at 50 DEG C carry out rate be 80r/min shaking table digest 15 minutes, cross 150 mesh sieves after, adopt It is that 2000r/min centrifuges carry out centrifugation and can obtain extract of soybean in 2 minutes with centrifugal rotational speed；
Second step adds in sealing high steam pot after fresh sericin fixation is cleaned up, then pass to 300 DEG C of steaming Vapour carries out boiling 5 minutes, then by cool to water is dried in room temperature, gravity-flow ventilation, 38 DEG C of dryer of addition carries out being dried to moisture content 15%；
3rd step with the material obtained after the processing of first and second step after crushing green bristlegrass, sweet tea napier grass, is subsequently added into mixing Beneficial bacterium is carried out after building heap fermentation 5 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned 3rd mixed fodder, diseases prevention liquid is prepared by following raw material：Thalictrum extract 10mg/ L, kuh-seng seed extract 20mg/l, oriental cherry bark extract 40mg/l, extractive of pubescent angelica root coumarin 50mg/l, oolong tea extract 60mg/l, forsythia suspense extraction 60mg/l, Flos Chrysanthemi Indici extract 50mg/l, Fructus Piperis extract 50mg/l；
In the diseases prevention liquid, Thalictrum extract is prepared by following methods：Grass of meadow rue is cleaned into crushing, is added in 50% (v/v) ethanol solution carries out heating and refluxing extraction 10 minutes at 50 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, The acetum for being subsequently added into 5 times of volumes adjusts pH to 4.5, at 55 DEG C, 100 hertz of high-voltage pulse electric field pulse processing 2 × 10-6S is extracted 20 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, ethyl acetate portion is taken to be concentrated under reduced pressure, Spray drying can obtain Thalictrum extract, and master contains alkaloid 48.21mg/g in extract；
The kuh-seng seed extract is prepared by following methods：Suitable silicon is added in after kuh-seng seed is cleaned Diatomaceous earth carries out mortar grinder into slurry, is subsequently added into 50% (v/v) ethanol solution and heating and refluxing extraction is carried out at 40 DEG C 5 minutes, Filtrate is filtered to take, filtrate is subsequently added into dilute hydrochloric acid and adjusts mistake after pH to 4.0 by the recycling ethyl alcohol that is concentrated under reduced pressure up to no alcohol taste Leaching supernatant is subsequently added into cellulase water enzyme digestion 20 minutes at 45 DEG C, is subsequently added into sodium hydroxide and adjusts pH into Property, it filters to take precipitation and can obtain kuh-seng seed extract, master contains total alkaloid 46.35mg/g in extract.
The oriental cherry bark extract is prepared by following methods：Oriental cherry bark is surpassed under pressure 3MPa 10 mesh sieves are crossed after Crushing of Ultrafine, 10 times of water refluxing extractions of addition are extracted 20 minutes at 50 DEG C obtains extracting solution, then adds in extraction The petroleum ether that liquid accumulates 1.2 times is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, is subsequently added into 80% (v/v) when ethanol solution progress alcohol extracting 1 is small, adsorbing separation elution then is carried out with macroreticular resin, volumetric concentration is used in elution successively Gradient elution, the alcohol-water of each gradient are carried out for 20%, 40%, 60%, 80% and 90% ethanol-water system eluent Solution elution volume is 5BV, elution flow rate 3BV/h, is concentrated under reduced pressure to give paste, dry to can obtain the extraction of flowering cherry skin Object, it is main in extract to contain coumaric acid glycosides 64.25mg/g；
The extractive of pubescent angelica root coumarin is prepared by following methods：White oil, diatomite grinding are added in after Radix Angelicae Pubescentis is cleaned Into slurry, 80% (v/v) ethanol solution refluxing extraction is then added in 3 times, the temperature extracted every time is 60 DEG C, the time of extraction is 30 Minute, merge the extracting solution of 3 extractions, add in filtering removal activated carbon after activated carbon decolorizing, added in just after being then diluted with water Butanol vibration rock after stand 5 minutes, then n-butanol fraction is taken to be separated with silica gel column chromatography, using petroleum ether-ethyl acetate as Eluant, eluent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give extractive of pubescent angelica root coumarin master containing Radix Angelicae Sinensis Lactone
19.35mg/g；The Radix Angelicae Pubescentis：White oil：Diatomaceous weight ratio is 2:0.05：0.1.
The oolong tea extract is prepared by following methods：Oolong tea drying is surpassed under pressure 3MPa 50 mesh sieves are crossed after Crushing of Ultrafine, impregnating oolong tea tea powder using 3 times of volumes, the water termostat that temperature is 100 DEG C obtains soup tea in 5 minutes, Vinegar acid for adjusting pH is subsequently added into 2.0, extracting solution is taken using 65% (v/v) ethanol solution refluxing extraction, then according to volume ratio For 1:1 ratio adds in ethyl acetate and is swayed standing extraction after twenty minutes, by ethyl acetate portion silica gel column chromatography point From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes Contain tea polyphenols 304.15mg/g to oolong tea extract master；
The forsythia suspense extraction is prepared by following methods：After Fructus Forsythiae is crushed, it is molten to add in 80% (v/v) ethyl alcohol Liquid carries out refluxing extraction 30 minutes under ultrasonic wave subsidiary conditions at 80 DEG C, and refluxing extraction 3 times, merges No. 3 extracting solutions altogether It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain forsythia suspense extraction master and contain epicatechin 22.34mg/g。
The Flos Chrysanthemi Indici extract is prepared by following methods：Wild chrysanthemum is added in 10 times of water and decocts 50 points Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.02 times of Flos Chrysanthemi Indici extracts that can obtain wild chrysanthemum active ingredient of original volume；
Fructus Piperis extract is prepared by following methods：White pepper is added in 10 times of water to decoct 50 minutes, mistake Leaching filtrate, reduction vaporization are concentrated into 1.02 times of Fructus Piperis extracts that can obtain white pepper active ingredient of original volume.
Embodiment 2：
A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses The solarization time reach 8 it is small when hillside as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more The solarization time reach 8 it is small when, 100 square metres of the area of every henhouse, henhouse spacing reach 800 meters or more, every longevity put in a suitable place to breed Fragrant chicken density is 1500,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 5 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched Longevity phase perfume (or spice) egg is to be positioned over nest on the rice straw after disinfection with 6, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period Between keep the coop ventilation amount to be 0.5m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed, The first time free-ranging since the 3rd day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out Column, the time into column, and send alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream Dynamic mountain spring water, 4 after fed with water start when small to the first mixed fodder of second month feeding, injected volume for 200g/ only；Second The moon started the second mixed fodder of feeding to four month, and injected volume is 300g/, when longevity perfume (or spice) chicken was at four month to seven month The 3rd mixed fodder of feeding, injected volume are 300g/；
First mixed fodder is mixed to get by following raw material：40 parts by weight of soy meal, 50 parts by weight of corn flour, chicken intestines 30 parts by weight of powder, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium acid carbonate, 0.6 parts by weight of fiery sesame oil；
Second mixed fodder is prepared by following raw material：30 parts by weight of soy meal, 20 parts by weight of corn flour, chicken intestines 18 parts by weight of powder, 8 parts by weight of amino acid, 2 parts by weight of salt, 1.0 parts by weight of sodium acid carbonate, 0.6 parts by weight of fiery sesame oil, pumpkin peel 15 parts by weight, 15 parts by weight of hayashishita weeds, 10 parts by weight of locust；
3rd mixed fodder is prepared by following raw material：25 parts by weight of dregs of beans, 20 parts by weight of wheat bran, 20 weight of fish meal Measure part, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium acid carbonate, 10 parts by weight of yellow meal worm, 15 weight of fermentation material Part, diseases prevention liquid 5.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is Only, the health care addition feed is prepared 50g/ by following raw material：20 parts by weight of dendrobium candidum, 3 parts by weight of bamboo shoot, sweet osmanthus 1.2 parts by weight, 1.0 parts by weight of mulberry leaf powder；
(6) deliver for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned 3rd mixed fodder：25 parts by weight of sweet tea napier grass, dog 115 parts by weight of tail grass, 15 parts by weight of sericin fixation, 25 parts by weight of extract of soybean, mixing 6 parts by weight of beneficial bacterium are (by following original Material is mixed to get：8 parts by weight of cellulase that viable count is 500,000,000 cfu/g, the Brevibacillus 6 that viable count is 800,000,000 cfu/g 4 parts by weight of saccharomyces pastorianus bacterium that parts by weight, viable count are 500,000,000 cfu/g, the bifidobacterium bifidum 5 that viable count is 800,000,000 cfu/g Parts by weight)；
One of raw material fermentation material is prepared by following methods in above-mentioned 3rd mixed fodder：
The water for selecting 6 times of volumes of good soybean addition is blended mashing and is fitted into conical flask for 15 minutes, then by the first step Add in 0.08% (w/v) sodium bicarbonate solution adjust pH to 6 obtain mixed serum, add 3% volume of slurries by 6 weight Part ficin, 5 parts by weight subtilopeptidase As, 5 parts by weight papains, 3 parts by weight bromelains composition Mixing protease in conical flask at 55 DEG C carry out rate be 100r/min shaking table digest 20 minutes, cross 150 mesh sieves after, adopt It is that 2500r/min centrifuges carry out centrifugation and can obtain extract of soybean in 5 minutes with centrifugal rotational speed；
Second step adds in sealing high steam pot after fresh sericin fixation is cleaned up, then pass to 500 DEG C of steaming Vapour carries out boiling 10 minutes, then by cool to water is dried in room temperature, gravity-flow ventilation, 40 DEG C of dryer of addition carries out being dried to moisture content 15-20%；
3rd step with the material obtained after the processing of first and second step after crushing green bristlegrass, sweet tea napier grass, is subsequently added into mixing Beneficial bacterium is carried out after building heap fermentation 8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned 3rd mixed fodder, diseases prevention liquid is prepared by following raw material：Thalictrum extract 20mg/ L, kuh-seng seed extract 30mg/l, oriental cherry bark extract 80mg/l, extractive of pubescent angelica root coumarin 70mg/l, oolong tea extract 90mg/l, forsythia suspense extraction 100mg/l, Flos Chrysanthemi Indici extract 70mg/l, Fructus Piperis extract 70mg/l；
In the diseases prevention liquid, Thalictrum extract is prepared by following methods：Grass of meadow rue is cleaned into crushing, is added in 55% (v/v) ethanol solution carries out heating and refluxing extraction 15 minutes at 55 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, The acetum for being subsequently added into 7 times of volumes adjusts pH to 5, at 55 DEG C, 110 hertz of high-voltage pulse electric field pulse processing 2.5 × 10-6S is extracted 25 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, ethyl acetate portion is taken to be concentrated under reduced pressure, Spray drying can obtain Thalictrum extract, and master contains alkaloid 64.22mg/g in extract；
The kuh-seng seed extract is prepared by following methods：Suitable silicon is added in after kuh-seng seed is cleaned Diatomaceous earth carries out mortar grinder into slurry, is subsequently added into 60% (v/v) ethanol solution and heating and refluxing extraction is carried out at 45 DEG C 7 minutes, Filtrate is filtered to take, filtrate is subsequently added into dilute hydrochloric acid and adjusts mistake after pH to 4.5 by the recycling ethyl alcohol that is concentrated under reduced pressure up to no alcohol taste Leaching supernatant is subsequently added into cellulase water enzyme digestion 25 minutes at 48 DEG C, is subsequently added into sodium hydroxide and adjusts pH into Property, it filters to take precipitation and can obtain kuh-seng seed extract, master contains total total alkaloid 51.35mg/g in extract.
The oriental cherry bark extract is prepared by following methods：Oriental cherry bark is surpassed under pressure 4MPa 15 mesh sieves are crossed after Crushing of Ultrafine, 15 times of water refluxing extractions of addition are extracted 25 minutes at 55 DEG C obtains extracting solution, then adds in extraction The petroleum ether that liquid accumulates 1.3 times is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, is subsequently added into 82% (v/v) when ethanol solution progress alcohol extracting 1.5 is small, adsorbing separation elution then is carried out with macroreticular resin, elution is dense with volume successively The ethanol-water system eluent spent for 20%, 40%, 60%, 80% and 90% carries out gradient elution, and the ethyl alcohol of each gradient- Aqueous solution elution volume is 5.5BV, elution flow rate 3.5BV/h, is concentrated under reduced pressure to give paste, dry to can obtain flowering cherry Bark extract, it is main in extract to contain coumaric acid glycosides 78.15mg/g；
The extractive of pubescent angelica root coumarin is prepared by following methods：White oil, diatomite grinding are added in after Radix Angelicae Pubescentis is cleaned Into slurry, 82% (v/v) ethanol solution refluxing extraction is then added in 3 times, the temperature extracted every time is 65 DEG C, the time of extraction is 35 Minute, merge the extracting solution of 3 extractions, add in filtering removal activated carbon after activated carbon decolorizing, added in just after being then diluted with water Butanol vibration rock after stand 7 minutes, then n-butanol fraction is taken to be separated with silica gel column chromatography, using petroleum ether-ethyl acetate as Eluant, eluent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give extractive of pubescent angelica root coumarin master containing Radix Angelicae Sinensis Lactone 27.54mg/g；The Radix Angelicae Pubescentis：White oil：Diatomaceous weight ratio is 2:0.06：0.2.
The oolong tea extract is prepared by following methods：Oolong tea drying is surpassed under pressure 4MPa 80 mesh sieves are crossed after Crushing of Ultrafine, impregnating oolong tea tea powder using 4 times of volumes, the water termostat that temperature is 110 DEG C obtains soup tea in 8 minutes, Vinegar acid for adjusting pH is subsequently added into 2.5, extracting solution is taken using 70% (v/v) ethanol solution refluxing extraction, then according to volume ratio For 1:1 ratio adds in ethyl acetate and carries out standing extraction after swaying 25 minutes, by ethyl acetate portion silica gel column chromatography point From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes Contain tea polyphenols 322.18mg/g to oolong tea extract master；
The forsythia suspense extraction is prepared by following methods：After Fructus Forsythiae is crushed, it is molten to add in 82% (v/v) ethyl alcohol Liquid carries out refluxing extraction 35 minutes under ultrasonic wave subsidiary conditions at 85 DEG C, and refluxing extraction 3 times, merges No. 3 extracting solutions altogether It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain forsythia suspense extraction master and contain epicatechin 28.64mg/g。
The Flos Chrysanthemi Indici extract is prepared by following methods：Wild chrysanthemum is added in 15 times of water and decocts 55 points Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.03 times of Flos Chrysanthemi Indici extracts that can obtain wild chrysanthemum active ingredient of original volume；
Fructus Piperis extract is prepared by following methods：White pepper is added in 18 times of water to decoct 55 minutes, mistake Leaching filtrate, reduction vaporization are concentrated into 1.04 times of Fructus Piperis extracts that can obtain white pepper active ingredient of original volume.
Embodiment 3：
A kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses The solarization time reach 7 it is small when hillside as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more The solarization time reach 7 it is small when, 90 square metres of the area of every henhouse, henhouse spacing reaches 800 meters or more, and every longevity for putting in a suitable place to breed is fragrant Chicken density is 1200,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 4 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched Longevity phase perfume (or spice) egg is to be positioned over nest on the rice straw after disinfection with 5, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period Between keep the coop ventilation amount to be 0.4m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed, The first time free-ranging since the 3rd day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out Column, the time into column, and send alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream Dynamic mountain spring water, 3.5 after fed with water start when small to the first mixed fodder of second month feeding, injected volume for 150g/ only；Second Start within a month to four month the second mixed fodder of feeding, injected volume is 250g/, when longevity perfume (or spice) chicken was at four month to the 7th Month the 3rd mixed fodder of feeding, injected volume for 250g/ only；
First mixed fodder is mixed to get by following raw material：30 parts by weight of soy meal, 40 parts by weight of corn flour, chicken intestines 20 parts by weight of powder, 5 parts by weight of amino acid, 0.6 parts by weight of salt, 0.6 parts by weight of sodium acid carbonate, 0.4 parts by weight of fiery sesame oil；
Second mixed fodder is prepared by following raw material：20 parts by weight of soy meal, 14 parts by weight of corn flour, chicken intestines 14 parts by weight of powder, 5 parts by weight of amino acid, 1.5 parts by weight of salt, 0.8 parts by weight of sodium acid carbonate, 0.4 parts by weight of fiery sesame oil, pumpkin 10 parts by weight of skin, 10 parts by weight of hayashishita weeds, 7 parts by weight of locust；
3rd mixed fodder is prepared by following raw material：18 parts by weight of dregs of beans, 13 parts by weight of wheat bran, 15 weight of fish meal Measure part, 6 parts by weight of amino acid, 0.8 parts by weight of salt, 0.5 parts by weight of sodium acid carbonate, 8 parts by weight of yellow meal worm, 10 weight of fermentation material Part, diseases prevention liquid 2.5g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is Only, the health care addition feed is prepared 25-50g/ by following raw material：13 parts by weight of dendrobium candidum, 2 parts by weight of bamboo shoot, 1.0 parts by weight of sweet osmanthus, 0.7 parts by weight of mulberry leaf powder；
(6) deliver for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned 3rd mixed fodder：20 parts by weight of sweet tea napier grass, dog 13 parts by weight of tail grass, 12 parts by weight of sericin fixation, 20 parts by weight of extract of soybean, mixing 5 parts by weight of beneficial bacterium are (by following raw material It is mixed to get：6 parts by weight of cellulase that viable count is 400,000,000 cfu/g, 5 weight of Brevibacillus that viable count is 600,000,000 cfu/g Measure part, 3 parts by weight of saccharomyces pastorianus bacterium that viable count is 300,000,000 cfu/g, 4 weight of bifidobacterium bifidum that viable count is 500,000,000 cfu/g Measure part)；
One of raw material fermentation material is prepared by following methods in above-mentioned 3rd mixed fodder：
The water for selecting 4 times of volumes of good soybean addition is blended mashing and is fitted into conical flask for 12 minutes, then by the first step Add in 0.06% (w/v) sodium bicarbonate solution adjust pH to 5.5 obtain mixed serum, add 2.5% volume of slurries by 4 Parts by weight ficin, 3 parts by weight subtilopeptidase As, 3 parts by weight papains, 2 parts by weight bromelains The mixing protease of composition carries out the shaking table that rate is 90r/min in conical flask and digests 17 minutes at 52 DEG C, cross 150 mesh sieves Afterwards, centrifugal rotational speed is used to carry out centrifugation for 2200r/min centrifuges and can obtain extract of soybean within 4 minutes；
Second step adds in sealing high steam pot after fresh sericin fixation is cleaned up, then pass to 400 DEG C of steaming Vapour carries out boiling 6 minutes, then by cool to water is dried in room temperature, gravity-flow ventilation, 39 DEG C of dryer of addition carries out being dried to moisture content 17%；
3rd step with the material obtained after the processing of first and second step after crushing green bristlegrass, sweet tea napier grass, is subsequently added into mixing Beneficial bacterium is carried out after building heap fermentation 7 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned 3rd mixed fodder, diseases prevention liquid is prepared by following raw material：Thalictrum extract 15mg/ L, kuh-seng seed extract 24mg/l, oriental cherry bark extract 65mg/l, extractive of pubescent angelica root coumarin 60mg/l, oolong tea extract 70mg/l, forsythia suspense extraction 80mg/l, Flos Chrysanthemi Indici extract 60mg/l, Fructus Piperis extract 60mg/l；
In the diseases prevention liquid, Thalictrum extract is prepared by following methods：Grass of meadow rue is cleaned into crushing, is added in 65% (v/v) ethanol solution carries out heating and refluxing extraction 20 minutes at 60 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, The acetum for being subsequently added into 10 times of volumes adjusts pH to 5.5, at 55 DEG C, 120 hertz of high-voltage pulse electric field pulse processing 3 × 10-6S is extracted 30 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, ethyl acetate portion is taken to be concentrated under reduced pressure, Spray drying can obtain Thalictrum extract, and master contains alkaloid 55.24mg/g in extract；
The kuh-seng seed extract is prepared by following methods：Suitable silicon is added in after kuh-seng seed is cleaned Diatomaceous earth carries out mortar grinder into slurry, is subsequently added into 65% (v/v) ethanol solution and heating and refluxing extraction is carried out at 50 DEG C 10 minutes, Filtrate is filtered to take, filtrate is subsequently added into dilute hydrochloric acid and adjusts mistake after pH to 5.0 by the recycling ethyl alcohol that is concentrated under reduced pressure up to no alcohol taste Leaching supernatant is subsequently added into cellulase water enzyme digestion 30 minutes at 50 DEG C, is subsequently added into sodium hydroxide and adjusts pH into Property, it filters to take precipitation and can obtain kuh-seng seed extract, master contains total alkaloid 48.25mg/g in extract.
The oriental cherry bark extract is prepared by following methods：Oriental cherry bark is surpassed under pressure 5MPa 20 mesh sieves are crossed after Crushing of Ultrafine, 20 times of water refluxing extractions of addition are extracted 30 minutes at 60 DEG C obtains extracting solution, then adds in extraction The petroleum ether that liquid accumulates 1.5 times is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, is subsequently added into 85% (v/v) when ethanol solution progress alcohol extracting 2 is small, adsorbing separation elution then is carried out with macroreticular resin, volumetric concentration is used in elution successively Gradient elution, the alcohol-water of each gradient are carried out for 20%, 40%, 60%, 80% and 90% ethanol-water system eluent Solution elution volume is 6BV, elution flow rate 4BV/h, is concentrated under reduced pressure to give paste, dry to can obtain the extraction of flowering cherry skin Object, it is main in extract to contain coumaric acid glycosides 72.12mg/g；
The extractive of pubescent angelica root coumarin is prepared by following methods：White oil, diatomite grinding are added in after Radix Angelicae Pubescentis is cleaned Into slurry, then add in 85% (v/v) ethanol solution refluxing extraction 3 times, the temperature extracted every time for DEG C, time of extraction be 40 points Clock merges the extracting solution of 3 extractions, adds in filtering removal activated carbon after activated carbon decolorizing, positive fourth is added in after being then diluted with water Alcohol vibration stands 10 minutes after rocking, and then n-butanol fraction is taken to be separated with silica gel column chromatography, using petroleum ether-ethyl acetate to wash De- agent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give extractive of pubescent angelica root coumarin master containing in Radix Angelicae Sinensis Ester 22.27mg/g；The Radix Angelicae Pubescentis：White oil：Diatomaceous weight ratio is 3:0.07：0.2.
The oolong tea extract is prepared by following methods：Oolong tea drying is surpassed under pressure 5MPa 100 mesh sieves are crossed after Crushing of Ultrafine, impregnating oolong tea tea powder using 5 times of volumes, the water termostat that temperature is 120 DEG C obtains soup in 10 minutes Tea is subsequently added into vinegar acid for adjusting pH to 3.0, extracting solution is taken using 75% (v/v) ethanol solution refluxing extraction, then according to volume Than for 1:1 ratio adds in ethyl acetate and carries out standing extraction after swaying 30 minutes, by ethyl acetate portion silica gel column chromatography Separation carries out gradient elution, 40 by eluant, eluent of petroleum ether-ethyl acetate：1 elution fraction carries out 5 recrystallizations with acetone It obtains oolong tea extract master and contains tea polyphenols 311.11mg/g；
The forsythia suspense extraction is prepared by following methods：After Fructus Forsythiae is crushed, it is molten to add in 85% (v/v) ethyl alcohol Liquid carries out refluxing extraction 40 minutes under ultrasonic wave subsidiary conditions at 90 DEG C, and refluxing extraction 3 times, merges No. 3 extracting solutions altogether It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain forsythia suspense extraction master and contain epicatechin 25.17mg/g。
The Flos Chrysanthemi Indici extract is prepared by following methods：Wild chrysanthemum is added in 20 times of water and decocts 60 points Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.05 times of Flos Chrysanthemi Indici extracts that can obtain wild chrysanthemum active ingredient of original volume；
Fructus Piperis extract is prepared by following methods：White pepper is added in 20 times of water to decoct 60 minutes, mistake Leaching filtrate, reduction vaporization are concentrated into 1.05 times of Fructus Piperis extracts that can obtain white pepper active ingredient of original volume.
Experiment one：
Comparative example 1：The preparation method of fermentation material：The first step, it is same as Example 1；Second step, by green bristlegrass, Si Kegao The material that fine strain of millet, sweet tea napier grass obtain after being handled after crushing with the first step is subsequently added into mixing beneficial bacterium and build after heap ferments 5 days i.e. Can, the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
Comparative example 2：The preparation method of fermentation material：Fresh soybean complete stool is cleaned up and carries out water and carry, concentrate by the first step Obtain extract of soybean；Second step, it is same as Example 1；3rd step, by green bristlegrass, sweet tea napier grass crush after at first and second step The material obtained after reason is subsequently added into mixing beneficial bacterium and carries out after building heap fermentation 5 days, is controlled during fermentation in fermentation heap Heart position is not more than 55 DEG C.
Comparative example 3：The preparation method of fermentation material：The first step, it is same as Example 1；Second step, it is same as Example 1；The Three steps are carried out with the material obtained after the processing of first and second step after building heap fermentation 5 days, hair after crushing green bristlegrass, sweet tea napier grass The center of fermentation heap is controlled to be not more than 55 DEG C during ferment.
Comparative example 4：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, Brevibacillus, viable count are identical with parts by weight；
Comparative example 5：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, saccharomyces pastorianus bacterium, viable count are identical with parts by weight；
Comparative example 6：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, bifidobacterium bifidum, viable count are identical with parts by weight；
Comparative example 7：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：3 parts by weight of Brevibacillus, saccharomyces pastorianus bacterium, bifidobacterium bifidum, viable count and parts by weight phase Together；
Comparative example 8：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, Brevibacillus, bifidobacterium bifidum, viable count are identical with parts by weight；
Comparative example 9：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, saccharomyces pastorianus bacterium, bifidobacterium bifidum, viable count are identical with parts by weight；
Comparative example 10：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original Material is mixed to get：Cellulase, Brevibacillus, saccharomyces pastorianus bacterium, viable count are identical with parts by weight；
Embodiment 1-3, control group 1-10 modes treated fermentation material are subjected to CN-Residual concentration detects, according to fermentation Cyanogen rate drops in being calculated containing cyanometer for fermentation material before and after the processing, wherein, drop cyanogen rate %=(concentration containing cyanogen-reactions in reaction primary fermentation material Concentration containing cyanogen in fermentation material afterwards)/concentration containing cyanogen in primary fermentation material is reacted, and Parallel testing three times is done in detection every time, is averaged Value.
Table 1
As seen from the above table, cyanogen rate drops in comparative example 1 compared with Example 1 reduces 30.3%, illustrates to carry out sericin fixation The content of phase generation cyanide after fermentation can be reduced after special processing, being the toxin of sericin fixation in itself will be before fermentation It can be removed, the expection before this imagines with experiment is consistent.Relatively drop cyanogen rate reduces comparative example 2 compared with Example 1 19.3%, it was demonstrated that the content higher containing dynamic element (sugar, protein) in extract of soybean Jing Guo special extraction is being fermented Phase provides one good growing environment of zymophyte, can give full play to the capacity of decomposition of flora；Comparative example 3 is compared with Example 1 53.8% is known clearly compared with drop cyanogen rate reduction, it was demonstrated that there is good capacity of decomposition between mixing beneficial bacterium, then in order to verify this sight Point, has done the contrast test of comparative example 4-10, and the single fermentation bacterium solution of comparative example 4-6 is reduced compared to embodiment 1-3 drop cyanogen abilities 20% or so；, comparative example 8-10 amount amount mixed bacteria liquid cyanogen ability dropped compared with embodiment 1-3 reduce 16% or so； It can be seen that the ability that there is mutually collaboration of complementing each other to drop cyanogen using the combined fermentation bacterium of the application, promotes to drop cyanogen ability significantly It improves.
Experiment two：
Using diseases prevention liquid prepared by the embodiment of the present invention 1 to the emergency toxicology experimental observation of longevity perfume (or spice) chicken
Experiment carries out in the clinical labororatory of ecological agriculture development Co., Ltd of Bama of Guangxi Green City.Experiment longevity perfume (or spice) chicken is purchased from Hechi City Jin Cheng Jiang Lao streets wholesale market, 1 kilogram or so of specification, experiment set 4 concentration gradient medicine groups altogether using enema (0.5g/l, 1.0g/l, 2.0g/l, 5.0g/l) and 1 blank control group for being not added with drug, every group sets 3 repetitions, and each group puts Longevity perfume (or spice) chicken 20, is specifically shown in Table 1.It after on-test, is observed continuously, records longevity perfume (or spice) chicken exception and death condition.
The emergency toxicology experiment grouping of 1 pair of longevity perfume (or spice) chicken
2 different pharmaceutical concentration lower longevity of table perfume (or spice) chicken death situation
It the results are shown in Table 2.Control group and 0.5g/l drug concentration groups are found dead and movable normal without longevity perfume (or spice) chicken 0.10g/l groups, average mortality reaches 8.33%, 0.20g/l groups after 96h, adds up average mortality 26.67%, 0.50g/l Group adds up average mortality 70.00%.
It is LD50=0.34745ml/m to longevity perfume (or spice) chicken half lethal dose to calculate the embodiment of the present invention 1 with Bliss methods3, Regression equation is y (Probit)=6.438+3.1321Log (D), is specifically shown in Table 3.By Te Lunbao formula (safe concentration= 48hLC50*0.3/ (LC/48hLC50 for 24 hours)) calculate the application liquid insecticide be to the safe concentration of longevity perfume (or spice) chicken 0.0955ml/m3, illustrate that the embodiment of the present invention 1 is higher to the security of longevity perfume (or spice) chicken.
3 Bliss methods of table calculate the embodiment of the present invention 1 to longevity perfume (or spice) chicken median lethal dose
Experiment three：
1.1 experiments kind
The longevity perfume (or spice) chicken (300 ± 20g of weight) of severe infections global-worm illness is purchased from Hechi City Jin Cheng Jiang Lao streets.Select equal spiritedness Bad, feather towers, head is crispaturaed, stand still for a long while an a corner, be off one's feed, cockscomb and visual mucous membrane anaemia, pale, gradual assistant, excrement The longevity perfume (or spice) chicken 1100 of appearance coccidia disease symptoms into water sample and with blood, is randomly assigned as 11 groups, every group of 100 longevity perfume (or spice) One henhouse of chicken.
The diseases prevention liquid bowel lavage that experimental group 1-3 is prepared respectively using embodiment 1-3, once, 20 in the least every time for bowel lavage on the one It rises；
Control group 1-8 is extracted respectively using only single containing Thalictrum extract, the extraction of kuh-seng seed, oriental cherry bark Object, extractive of pubescent angelica root coumarin, oolong tea extract, forsythia suspense extraction, Flos Chrysanthemi Indici extract, the anti-disease substance of Fructus Piperis extract are launched Enter henhouse, bowel lavage on the one once, 20 milliliters every time.
The death rate of observation longevity perfume (or spice) chicken was taken after 2 weeks,
Longevity perfume (or spice) chicken average mortality (%)=(longevity perfume (or spice) chicken amount of survival during longevity perfume (or spice) chicken quantity-microscopy of dispensing)/dispensing The sample highest effective concentration of longevity perfume (or spice) chicken quantity × 100%, i.e., the solvent extractable matter concentration corresponding to highest killing rate.
4 each groups of medicaments of table are to the control effect table of global-worm illness
Result of the test shows：There is excellent prevention to imitate the epidemic disease of the global-worm illness of longevity perfume (or spice) chicken using the diseases prevention liquid of the application Fruit, the extract for the control group 1-8 single uses that compare, which compares, not only has the effect of higher prevention global-worm illness, and desinsection It is better persistently, it was demonstrated that the formula of the application plays an important role of mutually to aid in cooperateing with.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (8)

1. a kind of cultural method of the longevity perfume (or spice) chicken with health-care efficacy, which is characterized in that specifically include following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses solarization Time reach 6-8 it is small when hillside as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse solarization on the hillside of 800 meters of water resource or more Time reach 6-8 it is small when, 80-100 square metres of the area of every henhouse, henhouse spacing reach 800 meters or more, every longevity put in a suitable place to breed Fragrant chicken density is 1000-1500,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, the incubation period Longevity perfume (or spice) egg is to be positioned over nest on the rice straw after disinfection with 4-6, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period Between keep the coop ventilation amount to be 0.2-0.5m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated by the way of full free-ranging；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed, from the longevity Start first time free-ranging within the 3rd day after the hatching of fragrant chicken, from free-ranging in first day start with the tinkle of bells training longevity perfume (or spice) chicken deliver for sale, into The time on column, and send alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination flowing mountain Spring, the 3-4 after fed with water start when small to the first mixed fodder of second month feeding, injected volume for 100-200g/ only；Second Start within a month to four month the second mixed fodder of feeding, injected volume for 200-300g/ only, when longevity perfume (or spice) chicken was at four month to the Seven months the 3rd mixed fodders of feeding, injected volume are 200-300g/；
First mixed fodder is mixed to get by following raw material：Soy meal 20-40 parts by weight, corn flour 30-50 parts by weight, chicken Rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium acid carbonate 0.3-0.8 parts by weight, fire fiber crops Oily 0.1-0.6 parts by weight；
Second mixed fodder is prepared by following raw material：Soy meal 15-30 parts by weight, corn flour 10-20 parts by weight, chicken Rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium acid carbonate 0.5-1.0 parts by weight, fiery sesame oil 0.2-0.6 parts by weight, pumpkin peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, locust 3-10 parts by weight；
3rd mixed fodder is prepared by following raw material：Dregs of beans 10-25 parts by weight, wheat bran 10-20 parts by weight, fish meal 10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium acid carbonate 0.3-0.8 parts by weight, yellow meal worm 5-10 Parts by weight, fermentation material 5-15 parts by weight, diseases prevention liquid 0.5-5.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed, injected volume 25- when five month big with addition of health care Only, the health care addition feed is prepared 50g/ by following raw material：Dendrobium candidum 10-20 parts by weight, bamboo shoot 1-3 weight Part, sweet osmanthus 0.5-1.2 parts by weight, mulberry leaf powder 0.5-1.0 parts by weight；
(6) deliver for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
2. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as described in claim 1, it is characterised in that：The fermentation material by Following raw material is prepared：Sweet tea napier grass 15-25 parts by weight, green bristlegrass 10-15 parts by weight, sericin fixation 10-15 parts by weight, soybean Extract 15-25 parts by weight, mixing beneficial bacterium 3-6 parts by weight.
3. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as claimed in claim 2, it is characterised in that：The mixing is beneficial Bacterium is mixed to get by following raw material：Cellulase 3-8 parts by weight, Brevibacillus 2-6 parts by weight, saccharomyces pastorianus bacterium 2-4 Parts by weight, bifidobacterium bifidum 2-5 parts by weight.
4. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as claimed in claim 2, it is characterised in that：The cellulase Viable count be hundred million cfu/g of 3-5, the viable count of Brevibacillus is hundred million cfu/g of 3-8, the viable count of saccharomyces pastorianus bacterium is Hundred million cfu/g of 2-5, the viable count of bifidobacterium bifidum are hundred million cfu/g of 3-8.
5. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as claimed in claim 2, it is characterised in that：The fermentation material is It is prepared by following methods：
The water for selecting 3-6 times of volume of good soybean addition is blended mashing and is fitted into conical flask for 10-15 minutes, connect by the first step The sodium bicarbonate solution adjusting pH to 5-6 for addition 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2-3% bodies Long-pending mixing protease carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieves, using centrifuge It carries out centrifugation and can obtain extract of soybean in 2-5 minutes；
Second step adds in sealing high steam pot after fresh sericin fixation is cleaned up, then pass to 300-500 DEG C of steaming Vapour carries out boiling 5-10 minutes, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition is dried to Moisture content 15-20%；
With the material obtained after the processing of first and second step after crushing green bristlegrass, sweet tea napier grass, it is beneficial to be subsequently added into mixing for 3rd step Bacterium is carried out after building heap fermentation 5-8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
6. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as claimed in claim 5, it is characterised in that：The mixed protein Enzyme is by 2-6 parts by weight ficin, 2-5 parts by weight subtilopeptidase A, 1-5 parts by weight papain, 1-3 weights Measure part bromelain composition.
7. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as claimed in claim 5, it is characterised in that：The speed of the shaking table Rate is 80-100r/min, and the centrifugal rotational speed of the centrifuge is 2000-2500r/min.
8. the cultural method of the longevity perfume (or spice) chicken with health-care efficacy as described in claim 1, it is characterised in that：The diseases prevention liquid by Following raw material is prepared：Thalictrum extract 10-20mg/l, kuh-seng seed extract 20-30mg/l, oriental cherry bark extract 40-80mg/l, extractive of pubescent angelica root coumarin 50-70mg/l, oolong tea extract 60-90mg/l, forsythia suspense extraction 60-100mg/l, mother chrysanthemum Flower extract 50-70mg/l, Fructus Piperis extract 50-70mg/l.
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