CN108308112A - A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken - Google Patents
A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken Download PDFInfo
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- CN108308112A CN108308112A CN201810093094.XA CN201810093094A CN108308112A CN 108308112 A CN108308112 A CN 108308112A CN 201810093094 A CN201810093094 A CN 201810093094A CN 108308112 A CN108308112 A CN 108308112A
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Abstract
The invention belongs to cultural technique fields, and in particular to a kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, including step (1) selection cultivation ground；(2) selection henhouse position：It selects building several henhouses on the hillside of 800 meters of water resource or more, every henhouse solarization time reaches 68 hours, 80 100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every longevity perfume (or spice) chicken density put in a suitable place to breed is 1,000 1500；(3) hatch；(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in such a way that free-ranging is with addition of stable breeding；(5) it delivers for sale.The longevity perfume (or spice) chicken yield of the cultivation of the present invention out reach 2.9 3.6 kilograms/only, deliver listing rate for sale and reach 93% or more, vitamin, protein, sugared content ratio on be improved, quality gets a promotion, and economic value content is high.
Description
【Technical field】
The invention belongs to cultural technique fields, and in particular to a kind of green cultivation method for the quality improving longevity perfume (or spice) chicken.
【Background technology】
Longevity perfume (or spice) chicken then enjoys the salubrious sweetness of the natural mountain spring water of bar horse, and seat gathers around 5000 mu of natural forest farms freely, enjoys gloomy
220 days natural growth cycles are spent in the favor of woods natural equilibrium food chain, perfection, are qualified " longevity township of world sylvogenic soil
Chicken " is known as " Gu member increases first of longevity chicken ", is to make poultry geographical sign star's product a bar horse future.
With the changes of the times, requirement of the people to food is higher and higher, for the progress kept pace with the times, promotes longevity perfume (or spice) chicken
Sales volume do not reduce, improve longevity perfume (or spice) chicken quality breeding way will be it is a kind of it is better improve longevity perfume (or spice) chicken economic value side
Method.
【Invention content】
The object of the present invention is to provide a kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, cultivation of the invention comes out
Longevity perfume (or spice) chicken yield reach 2.9-3.6 kilograms/only, deliver listing rate for sale and reach 94% or more, in vitamin, protein, sugared content
Ratio on be improved, quality gets a promotion, and economic value content is high.
To achieve the goals above, the technical solution adopted by the present invention is as follows：
A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, specifically includes following steps：
(1) selection cultivation ground：Selection has free of contamination water resource, possesses the tree of 6 years lifes above pine trees and miscellaneous tree miscegenation
Woods, possessing the solarization time reaches 6-8 hours hillside as farm；
(2) selection henhouse position：It selects building several henhouses on the hillside of 800 meters of water resource or more, every
The henhouse solarization time reaches 6-8 hours, 80-100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, every
The longevity perfume (or spice) chicken density put in a suitable place to breed is 1000-1500,
(3) hatched chicks：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and is taken out, continues to incubate to longevity perfume (or spice) chicken hen
Change, longevity incubation period perfume (or spice) egg is a to be positioned on the rice straw after disinfection with nest with 4-6, and longevity perfume (or spice) chicken hen of leaving freely is cooked
Nest, it is 0.2-0.5m that coop ventilation amount is kept during hatching3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in such a way that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
First time free-ranging third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken at noon and return the edible feed in hillside；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Mountain spring water is moved, the 3-4 hours after fed with water start to second month feeding first stage feed, and injected volume is 100-200g/；
Second month started feeding second stage feed to four month, and injected volume is 200-300g/, when longevity perfume (or spice) chicken was at four month
To seven month feeding phase III feed, injected volume is 200-300g/；
The first stage feed is mixed to get by following raw material：Corn protein powder 20-40 parts by weight, broad bean powder 30-50
Parts by weight, chicken rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 weights
Measure part, fiery sesame oil 0.1-0.6 parts by weight；
The second stage feed is prepared by following raw material：Corn protein powder 15-30 parts by weight, broad bean powder 10-20
Parts by weight, chicken rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium bicarbonate 0.5-1.0 parts by weight,
Fiery sesame oil 0.2-0.6 parts by weight, waxgourd peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, katydid 3-10 parts by weight；
The phase III feed is prepared by following raw material：Soyabean expeller 10-25 parts by weight, rice chaff 10-20 parts by weight,
Mountain flour 10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight, yellow meal worm
5-10 parts by weight, fermentation premix 5-15 parts by weight, diseases prevention liquid 1.0-10.0g/l；
When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is 20-30g/, the guarantor
Strong addition feed is prepared by following raw material：Smilax china L 20-35 parts by weight, Tricholoma 1-5 parts by weight, Radix zanthoxyli 0.5-1.0
Parts by weight, mulberry leaf powder 0.2-0.9 parts by weight；
(5) it delivers for sale：Dealing is delivered for sale in cultivation longevity perfume (or spice) chicken within seven month.
In the present invention, further instruction, the fermentation premix are prepared by following raw material：Rye grass 14-28
Parts by weight, hybridization green bristlegrass 12-22 parts by weight, manioc waste 10-22 parts by weight, Semen Lablab Album extract 8-15 parts by weight, beneficial to mixed
Combined bacteria 5-12 parts by weight；
In the present invention, further instruction, the beneficial Mixed Microbes are mixed to get by following raw material：Cellulase 5-10
Parts by weight, bacillus licheniformis 3-8 parts by weight, torulopsis 5-9 parts by weight, bifidobacterium longum 1-5 parts by weight.
In the present invention, the viable count of further instruction, the cellulase is hundred million cfu/g of 5-15, lichens gemma bar
The viable count of bacterium is hundred million cfu/g of 5-10, the viable count of torulopsis is hundred million cfu/g of 3-8, the viable count of bifidobacterium longum is 5-10
Hundred million cfu/g.
In the present invention, further instruction, the fermentation premix are prepared by following methods：
The water for selecting good Semen Lablab Album that 3-6 times of volume is added is blended 10-15 minutes loading conical flasks of mashing by the first step
In, the sodium bicarbonate solution adjusting pH to 5-6 for being subsequently added into 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2-
The mixing protease of 3% volume carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieve, use
Centrifuge carries out centrifugation and can be obtained Semen Lablab Album extract in 2-5 minutes；
Sealing high steam pot is added in manioc waste by second step, is then passed to 300-500 DEG C of steam and is carried out boiling 5-10
Minute, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition carries out being dried to moisture content 15-20% i.e.
It can；
Third walks, and the material obtained after being handled with the first and second step after hybridization green bristlegrass, rye grass are crushed is subsequently added into
It is carried out after building heap fermentation 5-8 days beneficial to Mixed Microbes, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
In the present invention, further instruction, the mixing protease are by 3-8 parts by weight subtilopeptidase A, 3-6
Parts by weight bromelain, 2-6 parts by weight ficin, 1-5 parts by weight papains composition.
In the present invention, the rate of further instruction, the shaking table is 80-100r/min, the centrifugation of the centrifuge
Rotating speed is 2000-2500r/min.
In the present invention, further instruction, the diseases prevention liquid are prepared by following raw material：Coptis extract 30-
60mg/l, Changchun flower extract 35-50mg/l, tonka beans extract 40-60mg/l, capillary extract 60-80mg/l, linseed
Extract 55-115mg/l, Flos Pelargonii Hortori 75-105mg/l, Cortex Moutan extract 50-70mg/l, cinnamomum cassia extract 30-
50mg/l。
It further illustrates in the present invention, the coptis extract is prepared by following methods：The coptis is cleaned
It crushes, 50-65% (v/v) ethanol solutions is added and carry out heating and refluxing extraction at 50-60 DEG C 10-20 minutes, extracting solution is taken to subtract
Pressure concentration adjusts pH to 4.5-5.5, at 55 DEG C, 100- up to no ethanol flavor, the acetum for being subsequently added into 5-10 times of volume
120 hertz of high-voltage pulse electric field pulse processing 2 × 10-6-3×10-6S is extracted 20-30 minutes, is obtained crude extract, then use
Ethyl acetate carries out layering extraction, and ethyl acetate portion is taken to be concentrated under reduced pressure, and is spray-dried and can be obtained coptis extract, in extract
It is main to contain alkaloid 35.12-40.28mg/g；
The Changchun flower extract is prepared by following methods：Suitable diatomite is added after catharanthus roseus is cleaned
It carries out mortar grinder to be slurried, is subsequently added into 50-65% (v/v) ethanol solutions and carries out heating and refluxing extraction 5-10 at 40-50 DEG C
Minute, filter to take filtrate, filtrate recycled into ethyl alcohol until no alcohol taste by reduced pressure, be subsequently added into dilute hydrochloric acid adjusting pH to
Supernatant is filtered to take after 4.0-5.0, is subsequently added into cellulase water enzyme digestion 20-30 minutes at 45-50 DEG C, is subsequently added into hydrogen
Sodium oxide molybdena adjusts pH to neutrality, filters to take precipitation and can be obtained Changchun flower extract, master contains total alkaloid in extract
35.65-41.02mg/g。
It further illustrates in the present invention, the tonka beans extract is prepared by following methods：By tonka beans
10-20 mesh sieve is crossed after carrying out ultramicro grinding at pressure 3-5MPa, 10-20 times of water refluxing extraction is added and is extracted at 50-60 DEG C
Obtain extracting solution within 20-30 minutes, the petroleum ether that 1.2-1.5 times of extracting liquid volume is then added is extracted, and petroleum ether portion is taken to connect
It and recycles petroleum ether with water-bath distillation, be subsequently added into 80-85% (v/v) ethanol solution and carry out alcohol extracting 1-2 hours, then use macropore
Resin carries out adsorbing separation elution, and it is 20%, 40%, 60%, 80% and 90% alcohol-water body that volumetric concentration is used in elution successively
It is that eluent carries out gradient elution, the ethanol-water solution elution volume of each gradient is 5-6BV, elution flow rate 3-4BV/h,
It is concentrated under reduced pressure to give paste, dry to can be obtained tonka beans extract, master contains coumaric acid glycosides 31.02- in extract
38.26mg/g；
The capillary extract is prepared by following methods：White oil, diatomite grinding are added after oriental wormwood is cleaned
Be slurried, 80-85% (v/v) ethanol solutions refluxing extractions be then added 3 times, the temperature extracted every time is 60-70 DEG C, extraction when
Between be 30-40 minute, merge 3 times extraction extracting solutions, be added activated carbon decolorizing after filtering remove activated carbon, be then diluted with water
It is added afterwards after n-butanol oscillation is rocked and stands 5-10 minutes, then n-butanol fraction is taken to be detached with silica gel column chromatography, with petroleum ether-
Ethyl acetate is that eluant, eluent carries out gradient elution, 40：1 elution fraction carries out 5 times with acetone and is recrystallized to give capillary extract
It is main to contain escoparone 20.36-31.04mg/g；The oriental wormwood：White oil：Diatomaceous weight ratio is 2-3:0.05-0.07：
0.1-0.2。
It further illustrates in the present invention, the extractive of flax seed is prepared by following methods：By linseed
It is dry to cross 50-100 mesh sieve after progress ultramicro grinding at pressure 3-5MPa, the use of 3-5 times of volume, temperature is 100-120 DEG C
Water termostat impregnates linseed tea powder and obtains soup tea in 5-10 minutes, is subsequently added into vinegar acid for adjusting pH to 2.0-3.0, uses 65-75%
(v/v) ethanol solution refluxing extraction takes extracting solution, is 1 then according to volume ratio:1 ratio is added ethyl acetate and is swayed
Extraction is stood after 20-30 minutes, ethyl acetate portion is detached with silica gel column chromatography, using petroleum ether-ethyl acetate as eluant, eluent
Gradient elution is carried out, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give extractive of flax seed master containing flax wood phenol
Plain 18.26-24.26mg/g；
The India extract is prepared by following methods：After India is crushed, 80-85% (v/v) second is added
Alcoholic solution carries out refluxing extraction 30-40 minutes under ultrasonic wave subsidiary conditions at 80-90 DEG C, and refluxing extraction 3 times altogether are closed
And No. 3 extracting solutions are concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can are obtained India extraction owner and contained
There is pelargonidin 21.06-35.27mg/g.
It further illustrates in the present invention, the Cortex Moutan extract is prepared by following methods：By moutan bark
10-20 times of water is added to decoct 50-60 minutes, filters to take filtrate, reduction vaporization, which is concentrated into 1.02-1.05 times of original volume, to be obtained
To the Cortex Moutan extract of moutan bark active constituent；
Cinnamomum cassia extract is prepared by following methods：Chinese cassia tree is added to 10-20 times of water to decoct 50-60 minutes,
Filtrate is filtered to take, reduction vaporization is concentrated into the cinnamomum cassia extract that 1.02-1.05 times of original volume can be obtained Chinese cassia tree active constituent.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. being cultivated using original free-ranging mode in the application, longevity perfume (or spice) chicken freely lives with enough during being cultivated
Dynamic time and space, can freely prey on, freely run so that the muscle of longevity perfume (or spice) chicken is sturdy, without fishy smell, chicken fat content is few, taste
Delicious and edible water be the mountain spring water that flows it is pollution-free it is few containing albic material, Mineral Elements Content is high, longevity perfume (or spice) chicken can be improved
Digestive system ability；By freely preying on, free of contamination mountain spring water living is eaten, reaches the cultivation of ecosystem, but also deposits
It is not high in the longevity perfume (or spice) chicken yield of original ecological breeding, because being easy infectious disease, delivered for sale in the epoch of this progress in order to reach raising
Yield, many farms are cultivated using the mode of stable breeding, not only output increased, and marketing time also shortens, to reach
To the economic benefit for improving businessman, but with the variation in epoch, people increasingly pursues quality, and therefore, this has been taken a fancy to by my company
The business opportunity of sample is found in continuous research, with addition of the mode of stable breeding and training feeding in the mode of free-ranging, can not only be improved
The yield delivered for sale, the longevity perfume (or spice) chicken delivered for sale also have very high health-care effect, substantially increase the quality of longevity perfume (or spice) chicken, and then greatly improve
The economic benefit of longevity perfume (or spice) chicken.
2. using different feeds is launched stage by stage, contain abundant protein, amino acid, dimension life in first stage feed
Element, with addition of fiery sesame oil can supplement linoleic acid content promote chicken growth, and then can fully absorb the protein in feed,
Amino acid, vitamin, and then the utilization rate of feed is improved, and the feed intake of chicken can be improved, achieve the purpose that not waste feed,
So that feed is reasonably utilized, the conversion ratio of feed is improved.In second stage feed, the ratio of the formula of feed is had adjusted
Example, with addition of waxgourd peel have the function of removing toxic substances after digestive system, promote to digest, and have the nutrition such as abundant starch at
Point, hayashishita weeds, which provide cellulose, can enhance digestion, and katydid is katydid living, can improve the grazing rate of chicken, and provide albumin
For the demand of growth, with addition of feed in achieved the purpose that make full use of agricultural waste material；It is each in phase III feed
Each performs its own functions for a raw material, and complements each other, and the nutritional ingredient provided adapts to use in the longevity perfume (or spice) chicken feed of growth 4-6 months, and nutrition is complete
Face is balanced, is capable of providing longevity perfume (or spice) chicken nutrient elements required for the growth, without slow-growing because nutrient is lacked, in turn
It ensure that the marketing time of longevity perfume (or spice) chicken.
3. harmful weeds be utilized turn waste into wealth in the fermentation premix of addition, be it is a kind of can utilization of resources weeds one
The new processing mode of kind, but manioc waste, which can digest to generate after hydrogen cyanide longevity perfume (or spice) chicken feed is used in being fermented, can cause brain, painstaking effort
The dysfunction of the systems such as pipe, therefore eat the cyaniding for needing further to carry out processing degradation manioc waste after being unable to direct fermentation
Object, the applicant by noxious material by that the study found that by the technological treatment of the application, rapidly can carry out detoxification drop
The toxicity of itself has been solved, has reduced the amount in fermentation release, and during the fermentation due to there is temperature to have microbial action to generate
Chemical reaction can cause a series of change of properties, generate some sorbefacient nutriments and promote growth of animal because
Son, not yet there are one detailed report principles therein so far, therefore it is that have very much must to carry out detoxification to raw material before fermentation
It wants, is found after detoxification by research so that noxious material burst size is reduced but not thoroughly in fermentation process, by multiple
The microorganism matched carries out the content that degradation substantially reduces noxious material, increases the safety of feed, and finds noxious material drop
The content of organics of feed increases after solution.
4. 8 kinds of extracts are respectively different active site extraction units in the diseases prevention liquid of the application, Each performs its own functions, Xiang Fuxiang
At synergistic effect plays the purpose of prevention longevity perfume (or spice) chicken coccidiasis, by containing life in the substance in the raw extract of the application
Alkaloids, phenols, tannin class, phenolic acid class, glycoside, are tested by the applicant and are matched, and the active constituent of each raw material complements each other
Reach the detoxication of toxicity synergistic and that alkaline matter can be released afterwards, 93% or more prevention longevity perfume is reached after compounding
The effect of morbidity and the poisoning of chicken coccidiasis, reduces the disease of globidiosis, and to reduce the disease rate of longevity perfume (or spice) chicken, increase is delivered for sale
Yield.
5. adding feed for feeding health care in 3 months before delivering for sale in the application, it is by fresh Smilax china L that feed is added in health care
With addition of the active polysaccharide extraction unit of Tricholoma, Radix zanthoxyli active polysaccharide extraction unit after being beaten, obtained after mulberry leaf powder mixing,
The health care adds feed longevity perfume (or spice) chicken feed with after 3 months, applicants have discovered that, the longevity perfume (or spice) chicken delivered for sale carries after culinary art in fresh meat
The fragrant of Smilax china L, the detection that nutrient composition content is carried out to the chicken nugget for the longevity perfume (or spice) chicken delivered for sale is found, is compared and is not being delivered for sale
The longevity perfume (or spice) chicken of health care addition feed is fed within first 3 months, amino acid content improves 3.96-4.56%, and stem of noble dendrobium alkali content improves
0.54-0.61%, protein content improve 9.24-10.23%, and sugared content improves 0.20-0.31%.
【Specific implementation mode】
Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with
Much implement different from other manner described here, those skilled in the art can be without prejudice to intension of the present invention the case where
Under do similar improvement, therefore the present invention is not limited to the specific embodiments disclosed below.
Embodiment 1：
A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, specifically includes following steps：
(1) selection cultivation ground：Selection has free of contamination water resource, possesses the tree of 6 years lifes above pine trees and miscellaneous tree miscegenation
Woods, possessing the solarization time reaches 6 hours hillside as farm；
(2) selection henhouse position：It selects building several henhouses on the hillside of 800 meters of water resource or more, every
The henhouse solarization time reaches 6 hours, 80 square metres of the area of every henhouse, henhouse spacing reaches 800 meters or more, and every is put in a suitable place to breed
Longevity perfume (or spice) chicken density is 1000,
(3) hatched chicks：Longevity perfume (or spice) egg is put into after hatching 3 days in incubator and is taken out, continues to hatch to longevity perfume (or spice) chicken hen,
Longevity incubation period perfume (or spice) egg with nest with 4 to be positioned on the rice straw after disinfection, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated
It is 0.2m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in such a way that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
First time free-ranging third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken at noon and return the edible feed in hillside；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Mountain spring water is moved, 3 hours after fed with water start to second month feeding first stage feed, and injected volume is 100g/；Second
The moon started feeding second stage feed to four month, and injected volume is 200g/, when longevity perfume (or spice) chicken was at four month to seven month
Feeding phase III feed, injected volume are 200g/；
The first stage feed is mixed to get by following raw material：20 parts by weight of corn protein powder, 30 parts by weight of broad bean powder,
10 parts by weight of chicken rice noodle roll end, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium bicarbonate, 0.1 parts by weight of fiery sesame oil；
The second stage feed is prepared by following raw material：15 parts by weight of corn protein powder, 10 parts by weight of broad bean powder,
10 parts by weight of chicken rice noodle roll end, 3 parts by weight of amino acid, 1 parts by weight of salt, 0.5 parts by weight of sodium bicarbonate, 0.2 parts by weight of fiery sesame oil, winter
5 parts by weight of melon skin, 5 parts by weight of hayashishita weeds, 3 parts by weight of katydid；
The phase III feed is prepared by following raw material：10 parts by weight of soyabean expeller, 10 parts by weight of rice chaff, mountain flour 10
Parts by weight, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium bicarbonate, 5 parts by weight of yellow meal worm, fermentation premix 5
Parts by weight, diseases prevention liquid 1.0g/l；
When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is 20g/, the health care
Addition feed is prepared by following raw material：20 parts by weight of Smilax china L, 1 parts by weight of Tricholoma, 0.5 parts by weight of Radix zanthoxyli, mulberry leaf
0.2 parts by weight of powder；
(5) it delivers for sale：Dealing is delivered for sale in cultivation longevity perfume (or spice) chicken within seven month.
One of raw material fermentation premix is prepared by following raw material in above-mentioned phase III feed：14 weight of rye grass
Part, hybridization green bristlegrass 12 parts by weight, 10 parts by weight of manioc waste, 8 parts by weight of Semen Lablab Album extract, beneficial to 5 parts by weight of Mixed Microbes (by
Following raw material is mixed to get：7 parts by weight of cellulase that viable count is 500,000,000 cfu/g, the lichens gemma that viable count is 500,000,000 cfu/g
5 parts by weight of bacillus, 6 parts by weight of torulopsis that viable count is 300,000,000 cfu/g, 3 weight of bifidobacterium longum that viable count is 500,000,000 cfu/g
Measure part)；
One of raw material fermentation premix is prepared by following methods in above-mentioned phase III feed：
The first step will select the water of 3 times of volumes of good Semen Lablab Album addition to blend mashing and be fitted into conical flask for 10 minutes, connects
Be added 0.05% (w/v) sodium bicarbonate solution adjust pH to 5 obtain mixed serum, add 2% volume of slurries by 3 weights
Measure part subtilopeptidase A, 3 parts by weight bromelains, 2 parts by weight ficins, 1 parts by weight papain group
At mixing protease in conical flask at 50 DEG C carry out rate be 80r/min shaking table digest 15 minutes, cross 150 mesh sieve after,
It uses centrifugal rotational speed to carry out centrifugation for 2000r/min centrifuges and can be obtained Semen Lablab Album extract in 2 minutes；
Sealing high steam pot is added in manioc waste by second step, is then passed to 300 DEG C of steam and is carried out boiling 5 minutes, connects
It cool to water is dried in room temperature, gravity-flow ventilation, 38 DEG C of dryer of addition carries out being dried to moisture content 15%；
Third walks, and the material obtained after being handled with the first and second step after hybridization green bristlegrass, rye grass are crushed is subsequently added into
It is carried out after building heap fermentation 5 days beneficial to Mixed Microbes, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
One of raw material in above-mentioned phase III feed, diseases prevention liquid is prepared by following raw material：Coptis extract 30mg/l,
Changchun flower extract 35mg/l, tonka beans extract 40mg/l, capillary extract 60mg/l, extractive of flax seed 55mg/l, day
Zhu Kui extracts 75mg/l, Cortex Moutan extract 50mg/l, cinnamomum cassia extract 30mg/l；
In the diseases prevention liquid, coptis extract is prepared by following methods：The coptis is cleaned and is crushed, is added 50%
(v/v) ethanol solution carries out heating and refluxing extraction 10 minutes at 50 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, then
The acetum that 5 times of volumes are added adjusts pH to 4.5, at 55 DEG C, 100 hertz of high-voltage pulse electric field pulse processing 2 × 10-6S,
Extraction 20 minutes, obtains crude extract, then carries out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure, spraying
Dry to can be obtained coptis extract, master contains alkaloid 35.12mg/g in extract；
The Changchun flower extract is prepared by following methods：Suitable diatomite is added after catharanthus roseus is cleaned
It carries out mortar grinder to be slurried, is subsequently added into 50% (v/v) ethanol solution and carries out heating and refluxing extraction at 40 DEG C 5 minutes, filter
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 4.0 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 20 minutes at 45 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can be obtained Changchun flower extract, and master contains total alkaloid 35.65mg/g in extract.
The tonka beans extract is prepared by following methods：Tonka beans are subjected to Ultramicro-powder at pressure 3MPa
10 mesh sieve is crossed after broken, 10 times of water refluxing extractions of addition are extracted 20 minutes at 50 DEG C obtains extracting solution, and extracting liq is then added
The petroleum ether of 1.2 times of product is extracted, and is taken petroleum ether portion then to recycle petroleum ether with water-bath distillation, is subsequently added into 80% (v/v)
Ethanol solution carries out alcohol extracting 1 hour, then carries out adsorbing separation elution with macroreticular resin, and elution is successively with volumetric concentration
20%, 40%, 60%, 80% and 90% ethanol-water system eluent carries out gradient elution, and the alcohol-water of each gradient is molten
Liquid elution volume is 5BV, elution flow rate 3BV/h, is concentrated under reduced pressure to give paste, and drying can be obtained tonka beans extract,
It is main in extract to contain coumaric acid glycosides 31.02mg/g；
The capillary extract is prepared by following methods：White oil, diatomite grinding are added after oriental wormwood is cleaned
It is slurried, 80% (v/v) ethanol solution refluxing extraction is then added 3 times, the temperature extracted every time is 60 DEG C, the time of extraction is 30
Minute, merge the extracting solution of 3 extractions, filtering removal activated carbon after activated carbon decolorizing is added, is added just after being then diluted with water
Butanol oscillation stands 5 minutes after rocking, and then n-butanol fraction is taken to be detached with silica gel column chromatography, is with petroleum ether-ethyl acetate
Eluant, eluent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give capillary extract master containing artemisia scoparia
Lactone
20.36mg/g；The oriental wormwood：White oil：Diatomaceous weight ratio is 2:0.05：0.1.
The extractive of flax seed is prepared by following methods：Linseed drying is surpassed at pressure 3MPa
50 mesh sieve is crossed after Crushing of Ultrafine, impregnating linseed tea powder using 3 times of volumes, the water termostat that temperature is 100 DEG C obtains soup tea in 5 minutes,
Vinegar acid for adjusting pH is subsequently added into 2.0, extracting solution is taken using 65% (v/v) ethanol solution refluxing extraction, then according to volume ratio
It is 1:1 ratio is added ethyl acetate and is swayed standing extraction after twenty minutes, by ethyl acetate portion silica gel column chromatography point
From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes
Contain flax lignan 18.26mg/g to extractive of flax seed master；
The India extract is prepared by following methods：After India is crushed, it is molten that 80% (v/v) ethyl alcohol is added
Liquid carries out refluxing extraction 30 minutes under ultrasonic wave subsidiary conditions at 80 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain India extraction owner and contain fish pelargonium color
Plain 21.06mg/g.
The Cortex Moutan extract is prepared by following methods：Moutan bark is added to 10 times of water and decocts 50 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into the Cortex Moutan extract that original volume 1.02 can be obtained moutan bark active constituent again；
Cinnamomum cassia extract is prepared by following methods：Chinese cassia tree is added to 10 times of water to decoct 50 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into the cinnamomum cassia extract that original volume 1.02 can be obtained Chinese cassia tree active constituent again.
Embodiment 2：
A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, specifically includes following steps：
(1) selection cultivation ground：Selection has free of contamination water resource, possesses the tree of 6 years lifes above pine trees and miscellaneous tree miscegenation
Woods, possessing the solarization time reaches 8 hours hillside as farm；
(2) selection henhouse position：It selects building several henhouses on the hillside of 800 meters of water resource or more, every
The henhouse solarization time reaches 8 hours, 100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every is put in a suitable place to breed
Longevity perfume (or spice) chicken density be 1500,
(3) hatched chicks：Longevity perfume (or spice) egg is put into after hatching 5 days in incubator and is taken out, continues to hatch to longevity perfume (or spice) chicken hen,
Longevity incubation period perfume (or spice) egg with nest with 6 to be positioned on the rice straw after disinfection, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated
It is 0.5m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in such a way that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
First time free-ranging third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken at noon and return the edible feed in hillside；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Mountain spring water is moved, 4 hours after fed with water start to second month feeding first stage feed, and injected volume is 200g/；Second
The moon started feeding second stage feed to four month, and injected volume is 300g/, when longevity perfume (or spice) chicken was at four month to seven month
Feeding phase III feed, injected volume are 300g/；
The first stage feed is mixed to get by following raw material：40 parts by weight of corn protein powder, 50 parts by weight of broad bean powder,
30 parts by weight of chicken rice noodle roll end, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, 0.6 parts by weight of fiery sesame oil；
The second stage feed is prepared by following raw material：30 parts by weight of corn protein powder, 20 parts by weight of broad bean powder,
18 parts by weight of chicken rice noodle roll end, 8 parts by weight of amino acid, 2 parts by weight of salt, 1.0 parts by weight of sodium bicarbonate, 0.6 parts by weight of fiery sesame oil, winter
15 parts by weight of melon skin, 15 parts by weight of hayashishita weeds, 10 parts by weight of katydid；
The phase III feed is prepared by following raw material：25 parts by weight of soyabean expeller, 20 parts by weight of rice chaff, mountain flour 20
Parts by weight, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, 10 parts by weight of yellow meal worm, fermentation premix
15 parts by weight, diseases prevention liquid 10.0g/l；
When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is 30g/, the health care
Addition feed is prepared by following raw material：35 parts by weight of Smilax china L, 5 parts by weight of Tricholoma, 1.0 parts by weight of Radix zanthoxyli, mulberry leaf
0.9 parts by weight of powder；
(5) it delivers for sale：Dealing is delivered for sale in cultivation longevity perfume (or spice) chicken within seven month.
One of raw material fermentation premix is prepared by following raw material in above-mentioned phase III feed：28 weight of rye grass
Part, 22 parts by weight of hybridization green bristlegrass, 22 parts by weight of manioc waste, 15 parts by weight of Semen Lablab Album extract, 12 parts by weight of beneficial Mixed Microbes
It (is mixed to get by following raw material：5 parts by weight of cellulase that viable count is 1,500,000,000 cfu/g, the ground that viable count is 1,000,000,000 cfu/g
The long bifid that 3 parts by weight of clothing bacillus, 5 parts by weight of torulopsis that viable count is 800,000,000 cfu/g, viable count are 1,000,000,000 cfu/g
1 parts by weight of bacillus)；
One of raw material fermentation premix is prepared by following methods in above-mentioned phase III feed：
The first step will select the water of 6 times of volumes of good Semen Lablab Album addition to blend mashing and be fitted into conical flask for 15 minutes, connects
Be added 0.08% (w/v) sodium bicarbonate solution adjust pH to 6 obtain mixed serum, add 3% volume of slurries by 6 weights
Measure part subtilopeptidase A, 4 parts by weight bromelains, 5 parts by weight ficins, 4 parts by weight papain groups
At mixing protease in conical flask at 55 DEG C carry out rate be 100r/min shaking table digest 20 minutes, cross 150 mesh sieve after,
It uses centrifugal rotational speed to carry out centrifugation for 2500r/min centrifuges and can be obtained Semen Lablab Album extract in 5 minutes；
Sealing high steam pot is added in manioc waste by second step, is then passed to 500 DEG C of steam and is carried out boiling 10 minutes,
Then by cool to water is dried in room temperature, gravity-flow ventilation, 40 DEG C of dryer of addition carries out being dried to moisture content 15-20%；
Third walks, and the material obtained after being handled with the first and second step after hybridization green bristlegrass, rye grass are crushed is subsequently added into
It is carried out after building heap fermentation 8 days beneficial to Mixed Microbes, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
One of raw material in above-mentioned phase III feed, diseases prevention liquid is prepared by following raw material：Coptis extract 60mg/l,
Changchun flower extract 50mg/l, tonka beans extract 60mg/l, capillary extract 80mg/l, extractive of flax seed 115mg/l, day
Zhu Kui extracts 105mg/l, Cortex Moutan extract 70mg/l, cinnamomum cassia extract 50mg/l；
In the diseases prevention liquid, coptis extract is prepared by following methods：The coptis is cleaned and is crushed, is added 55%
(v/v) ethanol solution carries out heating and refluxing extraction 15 minutes at 55 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, then
The acetum that 7 times of volumes are added adjusts pH to 5, at 55 DEG C, 110 hertz of high-voltage pulse electric field pulse processing 2.5 × 10-6S,
Extraction 25 minutes, obtains crude extract, then carries out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure, spraying
Dry to can be obtained coptis extract, master contains alkaloid 40.28mg/g in extract；
The Changchun flower extract is prepared by following methods：Suitable diatomite is added after catharanthus roseus is cleaned
It carries out mortar grinder to be slurried, is subsequently added into 60% (v/v) ethanol solution and carries out heating and refluxing extraction at 45 DEG C 7 minutes, filter
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 4.5 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 25 minutes at 48 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can be obtained Changchun flower extract, and master contains total alkaloid 41.02mg/g in extract.
The tonka beans extract is prepared by following methods：Tonka beans are subjected to Ultramicro-powder at pressure 4MPa
15 mesh sieve is crossed after broken, 15 times of water refluxing extractions of addition are extracted 25 minutes at 55 DEG C obtains extracting solution, and extracting liq is then added
The petroleum ether of 1.3 times of product is extracted, and is taken petroleum ether portion then to recycle petroleum ether with water-bath distillation, is subsequently added into 82% (v/v)
Ethanol solution carries out alcohol extracting 1.5 hours, then carries out adsorbing separation elution with macroreticular resin, and elution is successively with volumetric concentration
20%, 40%, 60%, 80% and 90% ethanol-water system eluent carries out gradient elution, and the alcohol-water of each gradient is molten
Liquid elution volume is 5.5BV, elution flow rate 3.5BV/h, is concentrated under reduced pressure to give paste, dry to can be obtained tonka beans extraction
Object, it is main in extract to contain coumaric acid glycosides 38.26mg/g；
The capillary extract is prepared by following methods：White oil, diatomite grinding are added after oriental wormwood is cleaned
It is slurried, 82% (v/v) ethanol solution refluxing extraction is then added 3 times, the temperature extracted every time is 65 DEG C, the time of extraction is 35
Minute, merge the extracting solution of 3 extractions, filtering removal activated carbon after activated carbon decolorizing is added, is added just after being then diluted with water
Butanol oscillation stands 7 minutes after rocking, and then n-butanol fraction is taken to be detached with silica gel column chromatography, is with petroleum ether-ethyl acetate
Eluant, eluent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give capillary extract master containing artemisia scoparia
Lactone
31.04mg/g；The oriental wormwood：White oil：Diatomaceous weight ratio is 2:0.06：0.2.
The extractive of flax seed is prepared by following methods：Linseed drying is surpassed at pressure 4MPa
80 mesh sieve is crossed after Crushing of Ultrafine, impregnating linseed tea powder using 4 times of volumes, the water termostat that temperature is 110 DEG C obtains soup tea in 8 minutes,
Vinegar acid for adjusting pH is subsequently added into 2.5, extracting solution is taken using 70% (v/v) ethanol solution refluxing extraction, then according to volume ratio
It is 1:1 ratio is added ethyl acetate and carries out standing extraction after swaying 25 minutes, by ethyl acetate portion silica gel column chromatography point
From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes
Contain flax lignan 24.26mg/g to extractive of flax seed master；
The India extract is prepared by following methods：After India is crushed, it is molten that 82% (v/v) ethyl alcohol is added
Liquid carries out refluxing extraction 35 minutes under ultrasonic wave subsidiary conditions at 85 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain India extraction owner and contain fish pelargonium color
Plain 35.27mg/g.
The Cortex Moutan extract is prepared by following methods：Moutan bark is added to 15 times of water and decocts 55 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into the Cortex Moutan extract that original volume 1.03 can be obtained moutan bark active constituent again；
Cinnamomum cassia extract is prepared by following methods：Chinese cassia tree is added to 18 times of water to decoct 55 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into the cinnamomum cassia extract that original volume 1.04 can be obtained Chinese cassia tree active constituent again.
Embodiment 3：
A kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, specifically includes following steps：
(1) selection cultivation ground：Selection has free of contamination water resource, possesses the tree of 6 years lifes above pine trees and miscellaneous tree miscegenation
Woods, possessing the solarization time reaches 7 hours hillside as farm；
(2) selection henhouse position：It selects building several henhouses on the hillside of 800 meters of water resource or more, every
The henhouse solarization time reaches 7 hours, 90 square metres of the area of every henhouse, henhouse spacing reaches 800 meters or more, and every is put in a suitable place to breed
Longevity perfume (or spice) chicken density is 1200,
(3) hatched chicks：Longevity perfume (or spice) egg is put into after hatching 4 days in incubator and is taken out, continues to hatch to longevity perfume (or spice) chicken hen,
Longevity incubation period perfume (or spice) egg with nest with 5 to be positioned on the rice straw after disinfection, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated
It is 0.4m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in such a way that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
First time free-ranging third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken at noon and return the edible feed in hillside；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Mountain spring water is moved, 3.5 hours after fed with water start to second month feeding first stage feed, and injected volume is 150g/；Second
Start within a month to four month feeding second stage feed, injected volume is 250g/, when longevity perfume (or spice) chicken was at four month to the 7th
Month feeding phase III feed, injected volume be 250g/ only；
The first stage feed is mixed to get by following raw material：30 parts by weight of corn protein powder, 40 parts by weight of broad bean powder,
20 parts by weight of chicken rice noodle roll end, 5 parts by weight of amino acid, 0.6 parts by weight of salt, 0.6 parts by weight of sodium bicarbonate, 0.4 parts by weight of fiery sesame oil；
The second stage feed is prepared by following raw material：20 parts by weight of corn protein powder, 14 parts by weight of broad bean powder,
14 parts by weight of chicken rice noodle roll end, 5 parts by weight of amino acid, 1.5 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, fiery 0.4 parts by weight of sesame oil,
10 parts by weight of waxgourd peel, 10 parts by weight of hayashishita weeds, 7 parts by weight of katydid；
The phase III feed is prepared by following raw material：18 parts by weight of soyabean expeller, 13 parts by weight of rice chaff, mountain flour 15
Parts by weight, 6 parts by weight of amino acid, 0.8 parts by weight of salt, 0.5 parts by weight of sodium bicarbonate, 8 parts by weight of yellow meal worm, fermentation premix 10
Parts by weight, diseases prevention liquid 5.0g/l；
When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is 25g/, the health care
Addition feed is prepared by following raw material：27 parts by weight of Smilax china L, 3 parts by weight of Tricholoma, 0.7 parts by weight of Radix zanthoxyli, mulberry leaf
0.5 parts by weight of powder；
(5) it delivers for sale：Dealing is delivered for sale in cultivation longevity perfume (or spice) chicken within seven month.
One of raw material fermentation premix is prepared by following raw material in above-mentioned phase III feed：21 weight of rye grass
Part, 19 parts by weight of hybridization green bristlegrass, 17 parts by weight of manioc waste, 10 parts by weight of Semen Lablab Album extract, 9 parts by weight of beneficial Mixed Microbes
It (is mixed to get by following raw material：10 parts by weight of cellulase that viable count is 1,100,000,000 cfu/g, the ground that viable count is 800,000,000 cfu/g
The long bifid that 8 parts by weight of clothing bacillus, 9 parts by weight of torulopsis that viable count is 500,000,000 cfu/g, viable count are 700,000,000 cfu/g
5 parts by weight of bacillus)；
One of raw material fermentation premix is prepared by following methods in above-mentioned phase III feed：
The first step will select the water of 4 times of volumes of good Semen Lablab Album addition to blend mashing and be fitted into conical flask for 12 minutes, connects
The sodium bicarbonate solution adjusting pH to 5.5 for 0.06% (w/v) of addition obtains mixed serum, adds 2.5% volume of slurries
By 8 parts by weight subtilopeptidase As, 6 parts by weight bromelains, 6 parts by weight ficins, 5 parts by weight Papains
The mixing protease of enzyme composition carries out the shaking table that rate is 90r/min in conical flask and digests 17 minutes at 52 DEG C, cross 150 mesh sieve
Afterwards, it uses centrifugal rotational speed to carry out centrifugation for 2200r/min centrifuges and can be obtained Semen Lablab Album extract in 4 minutes；
Sealing high steam pot is added in manioc waste by second step, is then passed to 400 DEG C of steam and is carried out boiling 6 minutes, connects
It cool to water is dried in room temperature, gravity-flow ventilation, 39 DEG C of dryer of addition carries out being dried to moisture content 17%；
Third walks, and the material obtained after being handled with the first and second step after hybridization green bristlegrass, rye grass are crushed is subsequently added into
It is carried out after building heap fermentation 7 days beneficial to Mixed Microbes, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
One of raw material in above-mentioned phase III feed, diseases prevention liquid is prepared by following raw material：Coptis extract 50mg/l,
Changchun flower extract 40mg/l, tonka beans extract 50mg/l, capillary extract 70mg/l, extractive of flax seed 85mg/l, day
Zhu Kui extracts 95mg/l, Cortex Moutan extract 60mg/l, cinnamomum cassia extract 45mg/l；
In the diseases prevention liquid, coptis extract is prepared by following methods：The coptis is cleaned and is crushed, is added 65%
(v/v) ethanol solution carries out heating and refluxing extraction 20 minutes at 60 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor, then
The acetum that 10 times of volumes are added adjusts pH to 5.5, at 55 DEG C, 120 hertz of high-voltage pulse electric field pulse processing 3 × 10- 6S is extracted 30 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure, spray
Mist drying can be obtained coptis extract, and master contains alkaloid 37.59mg/g in extract；
The Changchun flower extract is prepared by following methods：Suitable diatomite is added after catharanthus roseus is cleaned
It carries out mortar grinder to be slurried, is subsequently added into 65% (v/v) ethanol solution and carries out heating and refluxing extraction at 50 DEG C 10 minutes, filter
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 5.0 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 30 minutes at 50 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can be obtained Changchun flower extract, and master contains total alkaloid 37.23mg/g in extract.
The tonka beans extract is prepared by following methods：Tonka beans are subjected to Ultramicro-powder at pressure 5MPa
20 mesh sieve is crossed after broken, 20 times of water refluxing extractions of addition are extracted 30 minutes at 60 DEG C obtains extracting solution, and extracting liq is then added
The petroleum ether of 1.5 times of product is extracted, and is taken petroleum ether portion then to recycle petroleum ether with water-bath distillation, is subsequently added into 85% (v/v)
Ethanol solution carries out alcohol extracting 2 hours, then carries out adsorbing separation elution with macroreticular resin, and elution is successively with volumetric concentration
20%, 40%, 60%, 80% and 90% ethanol-water system eluent carries out gradient elution, and the alcohol-water of each gradient is molten
Liquid elution volume is 6BV, elution flow rate 4BV/h, is concentrated under reduced pressure to give paste, and drying can be obtained tonka beans extract,
It is main in extract to contain coumaric acid glycosides 33.24mg/g；
The capillary extract is prepared by following methods：White oil, diatomite grinding are added after oriental wormwood is cleaned
It is slurried, is then added 85% (v/v) ethanol solution refluxing extraction 3 times, the temperature extracted every time is DEG C, the time of extraction is 40 points
Clock merges the extracting solution of 3 extractions, and filtering removal activated carbon after activated carbon decolorizing is added, positive fourth is added after being then diluted with water
Alcohol oscillation stands 10 minutes after rocking, and then n-butanol fraction is taken to be detached with silica gel column chromatography, is to wash with petroleum ether-ethyl acetate
De- agent carries out gradient elution, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give capillary extract master containing in artemisia scoparia
Ester 24.27mg/g；The oriental wormwood：White oil：Diatomaceous weight ratio is 3:0.07：0.2.
The extractive of flax seed is prepared by following methods：Linseed drying is surpassed at pressure 5MPa
It is sieved with 100 mesh sieve after Crushing of Ultrafine, impregnating linseed tea powder using 5 times of volumes, the water termostat that temperature is 120 DEG C obtains soup in 10 minutes
Tea is subsequently added into vinegar acid for adjusting pH to 3.0, extracting solution is taken using 75% (v/v) ethanol solution refluxing extraction, then according to volume
Than being 1:1 ratio is added ethyl acetate and carries out standing extraction after swaying 30 minutes, by ethyl acetate portion silica gel column chromatography
Separation carries out gradient elution, 40 by eluant, eluent of petroleum ether-ethyl acetate：1 elution fraction carries out 5 recrystallizations with acetone
It obtains extractive of flax seed master and contains flax lignan 21.09mg/g；
The India extract is prepared by following methods：After India is crushed, it is molten that 85% (v/v) ethyl alcohol is added
Liquid carries out refluxing extraction 40 minutes under ultrasonic wave subsidiary conditions at 90 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain India extraction owner and contain fish pelargonium color
Plain 29.67mg/g.
The Cortex Moutan extract is prepared by following methods：Moutan bark is added to 20 times of water and decocts 60 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into the Cortex Moutan extract that original volume 1.05 can be obtained moutan bark active constituent again；
Cinnamomum cassia extract is prepared by following methods：Chinese cassia tree is added to 20 times of water to decoct 60 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into the cinnamomum cassia extract that original volume 1.05 can be obtained Chinese cassia tree active constituent again.
Experiment one：
Comparative example 1：The preparation method of fermentation premix：The first step, it is same as Example 1；Second step, by bandog tail
The material that grass, manioc waste, rye grass obtain after being handled with the first step after crushing is subsequently added into and carries out building heap fermentation beneficial to Mixed Microbes
After 5 days, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
Comparative example 2：The preparation method of fermentation premix：The first step, by fresh soybean complete stool clean up carry out water carry,
It is concentrated to give Semen Lablab Album extract；Second step, it is same as Example 1；Third walk, will hybridization green bristlegrass, rye grass crush after with
The material obtained after the processing of first and second step, which is subsequently added into, to carry out beneficial to Mixed Microbes after building heap fermentation 5 days, is controlled during fermentation
The center of fermentation heap is not more than 55 DEG C.
Comparative example 3：The preparation method of fermentation premix：The first step, it is same as Example 1；Second step, with 1 phase of embodiment
Together；Third walks, and carries out building heap fermentation 5 days with the material obtained after the processing of the first and second step after hybridizing green bristlegrass, rye grass crushing
Afterwards, the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
Comparative example 4：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, bacillus licheniformis, viable count are identical with parts by weight；
Comparative example 5：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, torulopsis, viable count are identical with parts by weight；
Comparative example 6：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, bifidobacterium longum, viable count are identical with parts by weight；
Comparative example 7：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Bacillus licheniformis, torulopsis, bifidobacterium longum, viable count are identical with parts by weight；
Comparative example 8：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, bacillus licheniformis, bifidobacterium longum, viable count are identical with parts by weight；
Comparative example 9：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, torulopsis, bifidobacterium longum, viable count are identical with parts by weight；
Comparative example 10：Ferment premix preparation method it is substantially same as Example 1, difference be beneficial to Mixed Microbes by with
Lower raw material is mixed to get：Cellulase, bacillus licheniformis, torulopsis, viable count are identical with parts by weight；
By embodiment 1-3, control group 1-10 modes treated fermentation premix carry out CN-Residual concentration detects, according to
The front and back fermentation premix of fermentation process calculates drop cyanogen rate containing cyanometer, wherein drop cyanogen rate %=(contains in reaction primary fermentation premix
Concentration containing cyanogen in fermentation premix after cyanogen concentration-reaction)/concentration containing cyanogen in primary fermentation premix is reacted, and detection is done every time
Parallel testing three times is averaged.
Table 1
As seen from the above table, cyanogen rate drops in comparative example 1 compared with Example 1 reduces 19.7%, illustrates to carry out manioc waste special
The content of phase generation cyanide after fermentation can be reduced after different processing, the as toxin of manioc waste itself will can be obtained before fermentation
To removing, the expection before this imagines with experiment is consistent.Relatively drop cyanogen rate reduces 17.4% to comparative example 2 compared with Example 1, card
The content higher containing dynamic element (sugar, protein) in the Semen Lablab Album extract Jing Guo special extraction is illustrated, is provided in the fermentation phase
One good growing environment of zymophyte, can give full play to the capacity of decomposition of flora；Cyanogen relatively drops in comparative example 3 compared with Example 1
Rate has reduced 54.2%, it was demonstrated that beneficial between Mixed Microbes there is good capacity of decomposition to be done then in order to verify this viewpoint
The single fermentation bacterium solution of the contrast test of comparative example 4-10, comparative example 4-6 reduces 25% left side compared to embodiment 1-3 drop cyanogen abilities
It is right；, comparative example 8-10 amount amount mixed bacteria liquid drop cyanogen ability compared with embodiment 1-3 and reduce 20% or so；It can be seen that
The ability that there is mutually collaboration of complementing each other to drop cyanogen using the combined fermentation bacterium of the application, promotes drop cyanogen ability to greatly improve.
Experiment two：
The diseases prevention liquid prepared using the embodiment of the present invention 1 is to the emergency toxicology experimental observation of longevity perfume (or spice) chicken
Experiment carries out in the clinical labororatory of ecological agriculture development Co., Ltd of Bama of Guangxi Green City.Experiment longevity perfume (or spice) chicken is purchased from
The Hechi City streets Jin Cheng Jiang Lao wholesale market, 1 kilogram or so of specification, experiment use enema, set 4 concentration gradient medicine groups altogether
(1.0g/l, 2.0g/l, 5.0g/l, 10.0g/l) and 1 blank control group for being not added with drug, every group sets 3 repetitions, each group
Longevity perfume (or spice) chicken 20 is put, is specifically shown in Table 1.It after on-test, is observed continuously, records longevity perfume (or spice) chicken exception and death condition.
The emergency toxicology experiment grouping of 1 pair of longevity perfume (or spice) chicken
2 different pharmaceutical concentration lower longevity of table perfume (or spice) chicken death situation
It the results are shown in Table 2.Control group and 1.0g/l drug concentration groups find death, and the normal 2.0g/ of activity without longevity perfume (or spice) chicken
L groups, average mortality reaches 10.0%, 5.0g/l groups after 96h, adds up average mortality 25.0%, and 10.0g/l groups add up flat
The equal death rate 56.67%.
It is LD50=0.34745ml/m to longevity perfume (or spice) chicken half lethal dose to calculate the embodiment of the present invention 1 with Bliss methods3,
Regression equation is y (Probit)=6.438+3.1321Log (D), is specifically shown in Table 3.By Te Lunbao formula (safe concentrations
=48hLC50*0.3/ (LC/48hLC50 for 24 hours)) calculate the application liquid insecticide it is dense to the safety of longevity perfume (or spice) chicken
Degree is
0.0955ml/m3, illustrate that the embodiment of the present invention 1 is higher to the safety of longevity perfume (or spice) chicken.
Table 3Bliss methods calculate the embodiment of the present invention 1 to longevity perfume (or spice) chicken median lethal dose
Experiment three：
1.1 experiments kind
The longevity perfume (or spice) chicken (500 ± 10g of weight) of severe infections globidiosis is purchased from the streets Hechi City Jin Cheng Jiang Lao.Selection has spirit
Bad, feather towers, head is crispaturaed, stand still for a long while an a corner, be off one's feed, cockscomb and visual mucous membrane anaemia, pale, gradual assistant, excrement
The longevity perfume (or spice) chicken 1100 of appearance coccidia disease symptoms at water sample and with blood, is randomly assigned to be 11 groups, every group of 100 longevity perfume (or spice)
One henhouse of chicken.
The diseases prevention liquid bowel lavage that experimental group 1-3 is prepared using embodiment 1-3 respectively, bowel lavage was primary on 1st, every time 20 milli
It rises；
Control group 1-8 contains coptis extract, catharanthus roseus extraction, tonka beans extract, oriental wormwood using only single respectively
Extract, extractive of flax seed, India extract, Cortex Moutan extract, cinnamomum cassia extract anti-disease substance launch into henhouse, one
Day bowel lavage is primary, 20 milliliters every time.
The death rate of observation longevity perfume (or spice) chicken is taken after 2 weeks,
Longevity perfume (or spice) chicken average mortality (%)=(longevity perfume (or spice) chicken amount of survival when longevity perfume (or spice) chicken quantity-microscopy of dispensing)/dispensing
Longevity perfume (or spice) chicken quantity × 100% sample highest effective concentration, i.e. solvent extractable matter concentration corresponding to highest killing rate.
Control effect table of the 4 each groups of medicaments of table to globidiosis
| Processing | Chicken number/only | Death toll/only | The death rate/% | Survival rate/% |
| Experimental group 1 | 100 | 6 | 6 | 94 |
| Experimental group 2 | 100 | 2 | 2 | 98 |
| Experimental group 3 | 100 | 7 | 7 | 93 |
| Control group 1 | 100 | 30 | 30 | 70 |
| Control group 2 | 100 | 31 | 31 | 69 |
| Control group 3 | 100 | 34 | 34 | 66 |
| Control group 4 | 100 | 32 | 32 | 68 |
| Control group 5 | 100 | 31 | 31 | 69 |
| Control group 6 | 100 | 34 | 34 | 66 |
| Control group 7 | 100 | 30 | 30 | 70 |
| Control group 8 | 100 | 33 | 33 | 67 |
Test result shows：There is excellent prevention to imitate the epidemic disease of the globidiosis of longevity perfume (or spice) chicken using the diseases prevention liquid of the application
Fruit, the extract for the control group 1-8 single uses that compare, which compares not only, has the effect of higher prevention globidiosis, and desinsection
It is better persistently, it was demonstrated that the formula of the application has the function of that mutually auxiliary cooperates with.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of green cultivation method for the quality improving longevity perfume (or spice) chicken, which is characterized in that specifically include following steps：
(1) selection cultivation ground：Selection has free of contamination water resource, possesses the woods of 6 years lifes above pine trees and miscellaneous tree miscegenation, gathers around
There is the hillside that the solarization time reaches 6-8 hours as farm；
(2) selection henhouse position：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more
The solarization time reaches 6-8 hours, 80-100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every is put in a suitable place to breed
Longevity perfume (or spice) chicken density be 1000-1500 only；
(3) hatched chicks：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and is taken out, continued to hatch to longevity perfume (or spice) chicken hen, incubate
Longevity change phase perfume (or spice) egg is a to be positioned on the rice straw after disinfection with nest with 4-6, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated
It is 0.2-0.5m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated by the way of free-ranging；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns first time free-ranging third day of the henhouse after longevity perfume (or spice) chicken hatches, from
Free-ranging in first day starts with the tinkle of bells training longevity perfume (or spice) chicken and delivers for sale, into the time on column, and it is fragrant to send out the alarm training longevity at noon
Chicken goes back to hillside and eats feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination flowing mountain
Spring, the 3-4 hours after fed with water start to second month feeding first stage feed, injected volume be 100-200g/ only；Second
Start within a month to four month feeding second stage feed, injected volume be 200-300g/ only, when longevity perfume (or spice) chicken was at four month to the
Seven months feeding phase IIIs feed, injected volume are 200-300g/；
The first stage feed is mixed to get by following raw material：Corn protein powder 20-40 parts by weight, broad bean powder 30-50 weight
Part, chicken rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight,
Fiery sesame oil 0.1-0.6 parts by weight；
The second stage feed is prepared by following raw material：Corn protein powder 15-30 parts by weight, broad bean powder 10-20 weight
Part, chicken rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium bicarbonate 0.5-1.0 parts by weight, fire fiber crops
Oily 0.2-0.6 parts by weight, waxgourd peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, katydid 3-10 parts by weight；
The phase III feed is prepared by following raw material：Soyabean expeller 10-25 parts by weight, rice chaff 10-20 parts by weight, mountain flour
10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight, yellow meal worm 5-10
Parts by weight, fermentation premix 5-15 parts by weight, diseases prevention liquid 1.0-10.0g/l；
When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is 20-30g/, and the health care adds
Feed is added to be prepared by following raw material：Smilax china L 20-35 parts by weight, Tricholoma 1-5 parts by weight, Radix zanthoxyli 0.5-1.0 weight
Part, mulberry leaf powder 0.2-0.9 parts by weight；
(5) it delivers for sale：Dealing is delivered for sale in cultivation longevity perfume (or spice) chicken within seven month.
2. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as described in claim 1, it is characterised in that：The fermentation premix
Material is prepared by following raw material：Rye grass 14-28 parts by weight, hybridization green bristlegrass 12-22 parts by weight, manioc waste 10-22 weight
Part, Semen Lablab Album extract 8-15 parts by weight, beneficial Mixed Microbes 5-12 parts by weight.
3. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The beneficial mixing
Bacterium is mixed to get by following raw material：Cellulase 5-10 parts by weight, bacillus licheniformis 3-8 parts by weight, torulopsis 5-9 weight
Part, bifidobacterium longum 1-5 parts by weight.
4. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The cellulase
Viable count be hundred million cfu/g of 5-15, the viable count of bacillus licheniformis is hundred million cfu/g of 5-10, the viable count of torulopsis is 3-8
Hundred million cfu/g, bifidobacterium longum viable count be hundred million cfu/g of 5-10.
5. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The fermentation premix
Material is prepared by following methods：
The first step will select the water of 3-6 times of volume of good Semen Lablab Album addition to blend mashing and be fitted into conical flask for 10-15 minutes,
The sodium bicarbonate solution adjusting pH to 5-6 for being subsequently added into 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2-3%
The mixing protease of volume carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieve, using centrifugation
Machine carries out centrifugation and can be obtained Semen Lablab Album extract in 2-5 minutes；
Sealing high steam pot is added in manioc waste by second step, is then passed to 300-500 DEG C of steam and is carried out 5-10 points of boiling
Clock, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition carries out being dried to moisture content 15-20% i.e.
It can；
Third walks, and the material obtained after being handled with the first and second step after hybridization green bristlegrass, rye grass are crushed is subsequently added into beneficial
Mixed Microbes carry out after building heap fermentation 5-8 days, and the center that fermentation heap is controlled during fermentation is not more than 55 DEG C.
6. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as claimed in claim 5, it is characterised in that：The mixed protein
Enzyme is by 3-8 parts by weight subtilopeptidase A, 3-6 parts by weight bromelain, 2-6 parts by weight ficin, 1-5 weights
Measure part papain composition.
7. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as claimed in claim 5, it is characterised in that：The speed of the shaking table
Rate is 80-100r/min, and the centrifugal rotational speed of the centrifuge is 2000-2500r/min.
8. improving the green cultivation method of the quality of longevity perfume (or spice) chicken as described in claim 1, it is characterised in that：The diseases prevention liquid by
Following raw material is prepared：Coptis extract 30-60mg/l, Changchun flower extract 35-50mg/l, tonka beans extract 40-
60mg/l, capillary extract 60-80mg/l, extractive of flax seed 55-115mg/l, Flos Pelargonii Hortori 75-105mg/l, tree peony
Bark extract 50-70mg/l, cinnamomum cassia extract 30-50mg/l.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744932A (en) * | 2008-12-15 | 2010-06-23 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating coccidiosis of poultry |
| CN106305602A (en) * | 2016-08-22 | 2017-01-11 | 安徽全红生态农庄有限公司 | Method for artificially breeding pheasants with low sickness rate and high healthcare value |
| CN106665477A (en) * | 2016-11-26 | 2017-05-17 | 阜阳金满地生态农业科技股份有限公司 | Breeding method of zinc-rich black-bone chickens |
| CN107549112A (en) * | 2017-10-23 | 2018-01-09 | 山西农业大学 | Put the cultural method of ecological chicken in a suitable place to breed in mountain region |
-
2018
- 2018-01-31 CN CN201810093094.XA patent/CN108308112A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744932A (en) * | 2008-12-15 | 2010-06-23 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating coccidiosis of poultry |
| CN106305602A (en) * | 2016-08-22 | 2017-01-11 | 安徽全红生态农庄有限公司 | Method for artificially breeding pheasants with low sickness rate and high healthcare value |
| CN106665477A (en) * | 2016-11-26 | 2017-05-17 | 阜阳金满地生态农业科技股份有限公司 | Breeding method of zinc-rich black-bone chickens |
| CN107549112A (en) * | 2017-10-23 | 2018-01-09 | 山西农业大学 | Put the cultural method of ecological chicken in a suitable place to breed in mountain region |
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