CN108142365A - A kind of cultural method of high-quality longevity perfume (or spice) chicken - Google Patents
A kind of cultural method of high-quality longevity perfume (or spice) chicken Download PDFInfo
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- CN108142365A CN108142365A CN201810093093.5A CN201810093093A CN108142365A CN 108142365 A CN108142365 A CN 108142365A CN 201810093093 A CN201810093093 A CN 201810093093A CN 108142365 A CN108142365 A CN 108142365A
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Abstract
The invention belongs to cultural technique fields, and in particular to a kind of cultural method of high-quality longevity perfume (or spice) chicken, including step (1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses the solarization time and reaches the hillside of 68 hours as farm；(2) henhouse construction；(3) hatch；(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；(5) health care cultivation is handled；(6) it delivers for sale.The longevity perfume (or spice) chicken yield of the cultivation of the present invention out reach 3.0 3.5 kilograms/only, deliver listing rate for sale and reach more than 94%, vitamin, protein, sugared content ratio on be improved, quality gets a promotion, and economic value content is high.
Description
【Technical field】
The invention belongs to cultural technique fields, and in particular to a kind of cultural method of high-quality longevity perfume (or spice) chicken.
【Background technology】
Longevity perfume (or spice) chicken then enjoys the salubrious sweetness of the natural mountain spring water of bar horse, and seat gathers around 5000 mu of natural forest farms freely, enjoys gloomy
220 days natural growth cycles are spent in the favor of woods natural equilibrium food chain, perfection, are qualified " longevity township of world sylvogenic soil
Chicken " is known as " Gu member increases first of longevity chicken ", is to make poultry geographical sign star's product a bar horse future.
With the changes of the times, requirement of the people to food is higher and higher, for the progress kept pace with the times, promotes longevity perfume (or spice) chicken
Sales volume do not reduce, improve longevity perfume (or spice) chicken quality breeding way will be it is a kind of it is better improve longevity perfume (or spice) chicken economic value side
Method.
【Invention content】
The object of the present invention is to provide a kind of cultural method of high-quality longevity perfume (or spice) chicken, the longevity perfume (or spice) chicken of cultivation of the invention out
Yield reach 3.0-3.5 kilograms/only, deliver listing rate for sale and reach more than 94%, vitamin, protein, sugared content ratio on
It is improved, quality gets a promotion, and economic value content is high.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of cultural method of high-quality longevity perfume (or spice) chicken, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses
The solarization time reaches the hillside of 6-8 hours as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more
The solarization time reaches 6-8 hours, 80-100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every is put in a suitable place to breed
Longevity perfume (or spice) chicken density for 1000-1500 only,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and taken out, continued to hatch to longevity perfume (or spice) chicken hen, incubate
Longevity change phase perfume (or spice) egg is to be positioned on the rice straw after disinfection with nest, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, is incubated with 4-6
It is 0.2-0.5m that coop ventilation amount is kept during change3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
The first time free-ranging since the third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Dynamic mountain spring water, 3-4 hours after fed with water start to second month feeding I mixed fodder, and injected volume is 100-200g/；
Second month started feeding Section II mixed fodder to four month, and injected volume is 200-300g/, when longevity perfume (or spice) chicken was at four month
To seven month feeding Section III mixed fodder, injected volume was 200-300g/；
The I mixed fodder is mixed to get by following raw material：Soy meal 20-40 parts by weight, corn flour 30-50 weight
Part, chicken rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight,
Fiery sesame oil 0.1-0.6 parts by weight；
The Section II mixed fodder is prepared by following raw material：Soy meal 15-30 parts by weight, corn flour 10-20 weight
Part, chicken rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium bicarbonate 0.5-1.0 parts by weight, fire fiber crops
Oily 0.2-0.6 parts by weight, pumpkin peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, mantis 3-10 parts by weight；
The Section III mixed fodder is prepared by following raw material：Cottonseed Meal 10-25 parts by weight, husk 10-20 weight
Part, bone meal 10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight, bloom
Worm 5-10 parts by weight, fermentation material 5-15 parts by weight, diseases prevention auxiliary liquid 1.0-10.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is
Only, the health care addition feed is prepared 20-30g/ by following raw material：Fleece-flower root 15-28 parts by weight, Russula delica 1-3 weight
Part, Caulis Spatholobi 0.8-1.5 parts by weight, mulberry leaf powder 0.3-0.8 parts by weight；
(6) it delivers for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
In the present invention, further instruction, the fermentation material are prepared by following raw material：Timothy grass 15-25 weight
Part, Bunge Corydalis Herb 10-20 parts by weight, bamboo shoot tip 8-15 parts by weight, Cauliflower extract 5-20 parts by weight, mixing beneficial bacterium 3-8 weights
Measure part；
In the present invention, further instruction, the mixing beneficial bacterium are mixed to get by following raw material：Cellulase 3-8
Parts by weight, bacillus subtilis 2-6 parts by weight, pichia 2-4 parts by weight, bifidobacterium pseudocatenulatum 2-5 parts by weight.
In the present invention, further instruction, the viable count of the cellulase is hundred million cfu/g of 5-15, bacillus subtilis
The viable count that the viable count of bacterium is hundred million cfu/g of 5-10, the viable count of pichia is hundred million cfu/g of 3-8, bifidobacterium pseudocatenulatum
For hundred million cfu/g of 5-10.
In the present invention, further instruction, the fermentation material are prepared by following methods：
The water that good Cauliflower is selected to add in 3-6 times of volume is blended 10-15 minutes loading conical flasks of mashing by the first step
In, the sodium bicarbonate solution adjusting pH to 5-6 for being subsequently added into 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2-
The mixing protease of 3% volume carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieve, use
Centrifuge carries out centrifugation and can obtain Cauliflower extract in 2-5 minutes；
Second step adds in sealing high steam pot, then passes to 300-500 DEG C after fresh bamboo shoot tip is cleaned up
Steam carries out boiling 5-10 minutes, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition is dried
To moisture content 15-20%；
Third walks, and with the material obtained after the processing of first and second step after Bunge Corydalis Herb, timothy grass are crushed, is subsequently added into mixing
Beneficial bacterium is carried out after building heap fermentation 5-8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
In the present invention, further instruction, the mixing protease are by 3-8 parts by weight subtilopeptidase A, 3-6
Parts by weight bromelain, 2-6 parts by weight ficin, 1-5 parts by weight papain composition.
In the present invention, further instruction, the rate of the shaking table are 80-100r/min, the centrifugation of the centrifuge
Rotating speed is 2000-2500r/min.
In the present invention, further instruction, the diseases prevention auxiliary liquid are prepared by following raw material：Belladonna extract
30-60mg/l, it henbane extract 30-60mg/l, vanilla extract 30-70mg/l, common cnidium fruit P.E 70-90mg/l, covers
Basin extract 65-105mg/l, Gentrin Knotweed P.E 80-115mg/l, Folium Artemisiae Argyi extract 50-70mg/l, galangal rhizome extract 40-
50mg/l。
It further illustrates in the present invention, the belladonna extract is prepared by following methods：Belladonna is cleaned
It crushes, adds in 50-65% (v/v) ethanol solutions and heating and refluxing extraction is carried out at 50-60 DEG C 10-20 minutes, extracting solution is taken to subtract
Pressure concentration adjusts pH to 4.5-5.5, at 55 DEG C, 100- up to no ethanol flavor, the acetum for being subsequently added into 5-10 times of volume
120 hertz of high-voltage pulse electric field pulse processing 2 × 10-6-3×10-6S is extracted 20-30 minutes, is obtained crude extract, then use
Ethyl acetate carries out layering extraction, and ethyl acetate portion is taken to be concentrated under reduced pressure, and spray drying is that can obtain belladonna extract, in extract
It is main to contain alkaloid 38.95-46.251mg/g；
The henbane extract is prepared by following methods：Suitable diatomite is added in after henbane seed is cleaned
It is slurry to carry out mortar grinder, is subsequently added into 50-65% (v/v) ethanol solutions and heating and refluxing extraction 5-10 is carried out at 40-50 DEG C
Minute, filter to take filtrate, filtrate recycled into ethyl alcohol until no alcohol taste by reduced pressure, be subsequently added into dilute hydrochloric acid adjusting pH to
Supernatant is filtered to take after 4.0-5.0, cellulase is subsequently added into water enzyme digestion 20-30 minutes at 45-50 DEG C, is subsequently added into hydrogen
Sodium oxide molybdena adjusts pH to neutrality, filters to take precipitation and can obtain henbane extract, master contains total alkaloid in extract
40.26-56.24mg/g。
It further illustrates in the present invention, the vanilla extract is prepared by following methods：Vanilla is being pressed
10-20 mesh sieve is crossed after ultramicro grinding is carried out under power 3-5MPa, 10-20 times of water refluxing extraction is added in and extracts 20-30 at 50-60 DEG C
Minute obtains extracting solution, and the petroleum ether for then adding in 1.2-1.5 times of extracting liquid volume is extracted, and petroleum ether portion is taken then to use water
Bath is distilled to recover petroleum ether, is subsequently added into 80-85% (v/v) ethanol solution and carries out alcohol extracting 1-2 hours, then with macroreticular resin into
Row adsorbing separation elutes, and elution is eluted successively with the ethanol-water system that volumetric concentration is 20%, 40%, 60%, 80% and 90%
Liquid carries out gradient elution, and the ethanol-water solution elution volume of each gradient is 5-6BV, elution flow rate 3-4BV/h, and decompression is dense
Contracting obtains paste, dry to can obtain vanilla extract, and master contains coumaric acid glycosides 40.26-51.23mg/g in extract；
The common cnidium fruit P.E is prepared by following methods：White oil, diatomite are added in after frutus cnidii is cleaned
It grinds slurry, then adds in 80-85% (v/v) ethanol solutions refluxing extractions 3 times, the temperature extracted every time is 60-70 DEG C, extraction
Time for 30-40 minute, merge the extracting solutions of 3 extractions, add in filtering removal activated carbon after activated carbon decolorizing, then add water
It is added in after dilution after n-butanol oscillation is rocked and stands 5-10 minutes, then n-butanol fraction is taken to be detached with silica gel column chromatography, with stone
Oily ether-ethyl acetate carries out gradient elution for eluant, eluent, 40：1 elution fraction carries out 5 times with acetone and is recrystallized to give cnidium monnieri
Seed extract master contains Osthole 15.24-25.31mg/g；The frutus cnidii：White oil：Diatomaceous weight ratio is 2-3:
0.05-0.07：0.1-0.2.
It further illustrates in the present invention, the Fructus Rubi extract is prepared by following methods：By raspberry
It is dry to cross 50-100 mesh sieve after progress ultramicro grinding under pressure 3-5MPa, the use of 3-5 times of volume, temperature is 100-120 DEG C
Water termostat impregnates raspberry tea powder and obtains soup tea in 5-10 minutes, is subsequently added into vinegar acid for adjusting pH to 2.0-3.0, uses 65-75%
(v/v) ethanol solution refluxing extraction takes extracting solution, is 1 then according to volume ratio:1 ratio adds in ethyl acetate and is swayed
Extraction is stood after 20-30 minutes, ethyl acetate portion is detached with silica gel column chromatography, using petroleum ether-ethyl acetate as eluant, eluent
Gradient elution is carried out, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give Fructus Rubi extract master containing Quercetin
12.26-25.36mg/g；
The Gentrin Knotweed P.E is prepared by following methods：After giant knotweed is crushed, 80-85% (v/v) second is added in
Alcoholic solution carries out refluxing extraction 30-40 minutes under ultrasonic wave subsidiary conditions at 80-90 DEG C, and refluxing extraction 3 times altogether are closed
And No. 3 extracting solutions are concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can are obtained Gentrin Knotweed P.E master and contained
There is resveratrol 18.36-27.31mg/g.
It further illustrates in the present invention, the galangal rhizome extract is prepared by following methods：By galangal
It adds in 10-20 times of water to decoct 50-60 minutes, filters to take filtrate, reduction vaporization, which is concentrated into 1.02-1.05 times of original volume, to be obtained
To the galangal rhizome extract of galangal active constituent；
Folium Artemisiae Argyi extract is prepared by following methods：Folium artemisiae argyi is added in 10-20 times of water to decoct 50-60 minutes,
Filtrate is filtered to take, reduction vaporization is concentrated into the Folium Artemisiae Argyi extract that 1.02-1.05 times of original volume can obtain folium artemisiae argyi active constituent.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. it is cultivated in the application using original free-ranging, when longevity perfume (or spice) chicken has enough freely activities during being cultivated
Between and space, can freely prey on, freely run so that the muscle of longevity perfume (or spice) chicken is sturdy, without fishy smell, chicken fat content is few, delicious flavour,
And edible water be that the mountain spring water flowed is pollution-free containing albic material is few, Mineral Elements Content is high, can improve disappearing for longevity perfume (or spice) chicken
The ability of change system；By freely preying on, free of contamination mountain spring water living is eaten, reaches the cultivation of ecosystem, but there is also originals
The longevity perfume (or spice) chicken yield of ecologic breeding is not high, because of easily infectious disease, the production delivered for sale is improved in order to reach in the epoch of this progress
Amount, many farms are cultivated using the mode of stable breeding, and not only output increased, and marketing time also shortens is carried with reaching
The economic benefit of high businessman, but with the variation in epoch, people increasingly pursues quality, therefore, my company has taken a fancy to such
Business opportunity is found in continuous research, with addition of the mode of stable breeding and training feeding in the mode of free-ranging, can not only be improved and be delivered for sale
Yield, the longevity perfume (or spice) chicken delivered for sale also have very high health-care effect, substantially increase the quality of longevity perfume (or spice) chicken, and then greatly improve Shou Xiang
The economic benefit of chicken.
2. using different feeds is launched stage by stage, contain abundant protein, amino acid, dimension life in I mixed fodder
Element, with addition of fiery sesame oil can supplement linoleic acid content promote chicken growth, and then can fully absorb the protein in feed,
Amino acid, vitamin, and then the utilization rate of feed is improved, and the feed intake of chicken can be improved, achieve the purpose that not waste feed,
So that feed is reasonably utilized, the conversion ratio of feed is improved.In Section II mixed fodder, the ratio of the formula of feed is had adjusted
Example, with addition of pumpkin peel after digestive system there is removing toxic substances, promote to digest, and have the nutrition such as abundant starch into
Point, hayashishita weeds, which provide cellulose, can enhance digestion, and mantis is mantis living, can improve the grazing rate of chicken, and provides albumin
For the demand of growth, with addition of feed in achieved the purpose that make full use of agricultural waste material；It is each in Section III mixed fodder
Each performs its own functions for a raw material, and complements each other, and the nutritional ingredient provided adapts to use in the longevity perfume (or spice) chicken feed of growth 4-6 months, and nutrition is complete
Face is balanced, is capable of providing longevity perfume (or spice) chicken nutrient elements required for the growth, without slow-growing because nutrient is lacked, and then
It ensure that the marketing time of longevity perfume (or spice) chicken.
It is that a kind of one kind of energy utilization of resources weeds is new 3. harmful weeds, which are utilized, in the fermentation material of addition turns waste into wealth
Processing mode, but bamboo shoot tip, which can digest to generate after hydrogen cyanide longevity perfume (or spice) chicken feed is used in being fermented, can cause brain, angiocarpy etc.
The dysfunction of system, therefore the cyanide for needing further to carry out processing degradation bamboo shoot tip is eaten after being unable to direct fermentation,
The applicant by noxious material by that the study found that by the technological treatment of the application, rapidly can carry out detoxification degradation
Toxicity itself reduces the amount in fermentation release, and during the fermentation due to the change for having temperature to have microbial action to generate
A series of change of properties can be caused by learning reaction, generated some sorbefacient nutriments and promoted the factor of growth of animal,
So far not yet there are one detailed report principle therein, therefore it is to be highly desirable to carry out detoxification to raw material before fermentation
, by research find after detoxification so that fermentation process in noxious material burst size reduce but not thoroughly, pass through compounding
Microorganism carry out degradation and substantially reduce the content of noxious material, increase the safety of feed, and find noxious material degradation
The content of organics of feed increases afterwards.
4. 8 kinds of extracts are respectively different active site extraction units in the diseases prevention auxiliary liquid of the application, Each performs its own functions, mutually auxiliary
It coordinates, synergistic effect plays the purpose of prevention longevity perfume (or spice) chicken coccidiasis, by containing in the substance in the raw extract of the application
Alkaloid, phenols, tannin class, phenolic acid class, glycoside are tested by the applicant and matched, the mutually auxiliary phase of active constituent of each raw material
Reach the detoxication of toxicity synergistic and that alkaline matter can be released after, reach for more than 94% prevention longevity after compounding
The effect of morbidity and the poisoning of fragrant chicken coccidiasis reduces the disease of global-worm illness, so as to reduce the disease rate of longevity perfume (or spice) chicken, increases and
The yield on column.
5. adding feed for feeding health care in 3 months before delivering for sale in the application, health care addition feed is by the fresh fleece-flower root
With addition of the active polysaccharide extraction unit of Russula delica, Caulis Spatholobi active polysaccharide extraction unit after being beaten, obtained after mulberry leaf powder mixing,
The health care adds feed longevity perfume (or spice) chicken feed with after 3 months, applicants have discovered that, the longevity perfume (or spice) chicken delivered for sale carries after culinary art in fresh meat
The fragrant of the fleece-flower root, the detection that nutrient composition content is carried out to the chicken nugget of longevity perfume (or spice) chicken delivered for sale is found, is compared and is not being delivered for sale
The longevity perfume (or spice) chicken of health care addition feed is fed within first 3 months, amino acid content improves 3.67-6.24%, and stem of noble dendrobium alkali content improves
0.84-0.97%, protein content improve 10.26-11.68%, and sugared content improves 0.25-0.64%.
【Specific embodiment】
Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with
Much implement different from other manner described here, those skilled in the art can be in the situation without prejudice to intension of the present invention
Under do similar improvement, therefore the present invention is not limited to the specific embodiments disclosed below.
Embodiment 1：
A kind of cultural method of high-quality longevity perfume (or spice) chicken, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses
The solarization time reaches the hillside of 6 hours as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more
The solarization time reaches 6 hours, 80 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every longevity put in a suitable place to breed is fragrant
Chicken density is 1000,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched
Longevity phase perfume (or spice) egg is to be positioned on the rice straw after disinfection with nest, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period with 4
Between keep the coop ventilation amount to be 0.2m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
The first time free-ranging since the third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Dynamic mountain spring water starts to second month feeding I mixed fodder after fed with water for 3 hours, injected volume for 100g/ only；Second
The moon started feeding Section II mixed fodder to four month, and injected volume is 200g/, when longevity perfume (or spice) chicken was at four month to seven month
Feeding Section III mixed fodder, injected volume are 200g/；
The I mixed fodder is mixed to get by following raw material：20 parts by weight of soy meal, 30 parts by weight of corn flour, chicken intestines
10 parts by weight of powder, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium bicarbonate, 0.1 parts by weight of fiery sesame oil；
The Section II mixed fodder is prepared by following raw material：15 parts by weight of soy meal, 10 parts by weight of corn flour, chicken intestines
10 parts by weight of powder, 3 parts by weight of amino acid, 1 parts by weight of salt, 0.5 parts by weight of sodium bicarbonate, 0.2 parts by weight of fiery sesame oil, pumpkin peel
5 parts by weight, 5 parts by weight of hayashishita weeds, 3 parts by weight of mantis；
The Section III mixed fodder is prepared by following raw material：10 parts by weight of Cottonseed Meal, 10 parts by weight of husk, bone meal
10 parts by weight, 3 parts by weight of amino acid, 0.5 parts by weight of salt, 0.3 parts by weight of sodium bicarbonate, 5 parts by weight of yellow meal worm, 5 weight of fermentation material
Measure part, diseases prevention auxiliary liquid 1.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is
Only, the health care addition feed is prepared 20g/ by following raw material：15 parts by weight of the fleece-flower root, 1 parts by weight of Russula delica, Caulis Spatholobi
0.8 parts by weight, 0.3 parts by weight of mulberry leaf powder；
(6) it delivers for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned Section III mixed fodder：15 parts by weight of timothy grass,
10 parts by weight of fourth grass, 8 parts by weight of bamboo shoot tip, 5 parts by weight of Cauliflower extract, mixing 3 parts by weight of beneficial bacterium (are mixed by following raw material
Conjunction obtains：3 parts by weight of cellulase that viable count is 500,000,000 cfu/g, 2 weight of bacillus subtilis that viable count is 500,000,000 cfu/g
Part, 2 parts by weight of pichia that viable count is 300,000,000 cfu/g, 2 weight of bifidobacterium pseudocatenulatum that viable count is 500,000,000 cfu/g
Part)；
One of raw material fermentation material is prepared by following methods in above-mentioned Section III mixed fodder：
The water for selecting 3 times of volumes of good Cauliflower addition is blended mashing and is fitted into conical flask for 10 minutes, connect by the first step
Add in 0.05% (w/v) sodium bicarbonate solution adjust pH to 5 obtain mixed serum, add 2% volume of slurries by 3 weights
Measure part subtilopeptidase A, 3 parts by weight bromelains, 2 parts by weight ficins, 1 parts by weight papain group
Into mixing protease in conical flask at 50 DEG C carry out rate be 80r/min shaking table digest 15 minutes, cross 150 mesh sieve after,
Centrifugal rotational speed is used to carry out centrifugation for 2000r/min centrifuges and can obtain Cauliflower extract within 2 minutes；
Second step adds in sealing high steam pot after fresh bamboo shoot tip is cleaned up, then pass to 300 DEG C of steam
Boiling 5 minutes is carried out, then by cool to water is dried in room temperature, gravity-flow ventilation, 38 DEG C of dryer of addition carries out being dried to moisture content
15%；
Third walks, and with the material obtained after the processing of first and second step after Bunge Corydalis Herb, timothy grass are crushed, is subsequently added into mixing
Beneficial bacterium is carried out after building heap fermentation 5 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned Section III mixed fodder, diseases prevention auxiliary liquid are prepared by following raw material：Belladonna extract
30mg/l, henbane extract 30mg/l, vanilla extract 30mg/l, common cnidium fruit P.E 70mg/l, raspberry
Extract 65mg/l, Gentrin Knotweed P.E 80mg/l, Folium Artemisiae Argyi extract 50mg/l, galangal rhizome extract 40mg/l；
In the diseases prevention auxiliary liquid, belladonna extract is prepared by following methods：Belladonna is cleaned into crushing, is added in
50% (v/v) ethanol solution carries out heating and refluxing extraction 10 minutes at 50 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor,
The acetum for being subsequently added into 5 times of volumes adjusts pH to 4.5, at 55 DEG C, 100 hertz of high-voltage pulse electric field pulse processing 2 ×
10-6S is extracted 20 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure,
Spray drying can obtain belladonna extract, and master contains alkaloid 38.95mg/g in extract；
The henbane extract is prepared by following methods：Suitable diatomite is added in after henbane seed is cleaned
It is slurry to carry out mortar grinder, is subsequently added into 50% (v/v) ethanol solution and heating and refluxing extraction is carried out at 40 DEG C 5 minutes, filters
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 4.0 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 20 minutes at 45 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can obtain henbane extract, and master contains total alkaloid 40.26mg/g in extract.
The vanilla extract is prepared by following methods：After vanilla is carried out ultramicro grinding under pressure 3MPa
10 mesh sieve is crossed, 10 times of water refluxing extractions of addition are extracted 20 minutes at 50 DEG C obtains extracting solution, then adds in extracting liquid volume 1.2
Petroleum ether again is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, it is molten to be subsequently added into 80% (v/v) ethyl alcohol
Liquid carries out alcohol extracting 1 hour, then carries out adsorbing separation elution with macroreticular resin, elution is successively 20% with volumetric concentration, 40%,
60%th, 80% and 90% ethanol-water system eluent carries out gradient elution, the ethanol-water solution elution volume of each gradient
For 5BV, elution flow rate 3BV/h, paste is concentrated under reduced pressure to give, dry to can obtain vanilla extract, master contains in extract
There is coumaric acid glycosides 40.26mg/g；
The common cnidium fruit P.E is prepared by following methods：White oil, diatomite are added in after frutus cnidii is cleaned
It grinds slurry, then adds in 80% (v/v) ethanol solution refluxing extraction 3 times, the temperature extracted every time is 60 DEG C, the time of extraction
It it is 30 minutes, the extracting solutions of 3 extractions of merging add in filtering removal activated carbon after activated carbon decolorizing, add after being then diluted with water
Enter after n-butanol oscillation is rocked and stand 5 minutes, then n-butanol fraction is taken to be detached with silica gel column chromatography, with petroleum ether-acetic acid second
Ester carries out gradient elution for eluant, eluent, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give common cnidium fruit P.E master containing
There is Osthole 15.24mg/g；The frutus cnidii：White oil：Diatomaceous weight ratio is 2:0.05：0.1.
The Fructus Rubi extract is prepared by following methods：Raspberry drying is surpassed under pressure 3MPa
50 mesh sieve is crossed after Crushing of Ultrafine, impregnating raspberry tea powder using 3 times of volumes, the water termostat that temperature is 100 DEG C obtains soup tea in 5 minutes,
Vinegar acid for adjusting pH is subsequently added into 2.0, extracting solution is taken using 65% (v/v) ethanol solution refluxing extraction, then according to volume ratio
It is 1:1 ratio adds in ethyl acetate and is swayed standing extraction after twenty minutes, by ethyl acetate portion silica gel column chromatography point
From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes
Contain Quercetin 12.26mg/g to Fructus Rubi extract master；
The Gentrin Knotweed P.E is prepared by following methods：After giant knotweed is crushed, it is molten to add in 80% (v/v) ethyl alcohol
Liquid carries out refluxing extraction 30 minutes under ultrasonic wave subsidiary conditions at 80 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain Gentrin Knotweed P.E master and contain resveratrol
18.36mg/g。
The galangal rhizome extract is prepared by following methods：Galangal is added in 10 times of water and decocts 50 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.02 times of galangal rhizome extracts that can obtain galangal active constituent of original volume；
Folium Artemisiae Argyi extract is prepared by following methods：Folium artemisiae argyi is added in 10 times of water to decoct 50 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into 1.02 times of Folium Artemisiae Argyi extracts that can obtain folium artemisiae argyi active constituent of original volume.
Embodiment 2：
A kind of cultural method of high-quality longevity perfume (or spice) chicken, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses
The solarization time reaches the hillside of 8 hours as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more
The solarization time reaches 8 hours, 100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, every longevity put in a suitable place to breed
Fragrant chicken density is 1500,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 5 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched
Longevity phase perfume (or spice) egg is to be positioned on the rice straw after disinfection with nest, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period with 6
Between keep the coop ventilation amount to be 0.5m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
The first time free-ranging since the third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Dynamic mountain spring water starts to second month feeding I mixed fodder after fed with water for 4 hours, injected volume for 200g/ only；Second
The moon started feeding Section II mixed fodder to four month, and injected volume is 300g/, when longevity perfume (or spice) chicken was at four month to seven month
Feeding Section III mixed fodder, injected volume are 300g/；
The I mixed fodder is mixed to get by following raw material：40 parts by weight of soy meal, 50 parts by weight of corn flour, chicken intestines
30 parts by weight of powder, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, 0.6 parts by weight of fiery sesame oil；
The Section II mixed fodder is prepared by following raw material：30 parts by weight of soy meal, 20 parts by weight of corn flour, chicken intestines
18 parts by weight of powder, 8 parts by weight of amino acid, 2 parts by weight of salt, 1.0 parts by weight of sodium bicarbonate, 0.6 parts by weight of fiery sesame oil, pumpkin peel
15 parts by weight, 15 parts by weight of hayashishita weeds, 10 parts by weight of mantis；
The Section III mixed fodder is prepared by following raw material：25 parts by weight of Cottonseed Meal, 20 parts by weight of husk, bone meal
20 parts by weight, 8 parts by weight of amino acid, 1.0 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, 10 parts by weight of yellow meal worm, fermentation material 15
Parts by weight, diseases prevention auxiliary liquid 10.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is
Only, the health care addition feed is prepared 30g/ by following raw material：28 parts by weight of the fleece-flower root, 3 parts by weight of Russula delica, Caulis Spatholobi
1.5 parts by weight, 0.8 parts by weight of mulberry leaf powder；
(6) it delivers for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned Section III mixed fodder：25 parts by weight of timothy grass,
20 parts by weight of fourth grass, 15 parts by weight of bamboo shoot tip, 20 parts by weight of Cauliflower extract, mixing 8 parts by weight of beneficial bacterium are (by following raw material
It is mixed to get：3 parts by weight of cellulase that viable count is 1,500,000,000 cfu/g, the bacillus subtilis 2 that viable count is 1,000,000,000 cfu/g
2 parts by weight of pichia that parts by weight, viable count are 800,000,000 cfu/g, the bifidobacterium pseudocatenulatum 2 that viable count is 1,000,000,000 cfu/g
Parts by weight)；
One of raw material fermentation material is prepared by following methods in above-mentioned Section III mixed fodder：
The water for selecting 6 times of volumes of good Cauliflower addition is blended mashing and is fitted into conical flask for 15 minutes, connect by the first step
Add in 0.08% (w/v) sodium bicarbonate solution adjust pH to 6 obtain mixed serum, add 3% volume of slurries by 6 weights
Measure part subtilopeptidase A, 4 parts by weight bromelains, 5 parts by weight ficins, 4 parts by weight papain groups
Into mixing protease in conical flask at 55 DEG C carry out rate be 100r/min shaking table digest 20 minutes, cross 150 mesh sieve after,
Centrifugal rotational speed is used to carry out centrifugation for 2500r/min centrifuges and can obtain Cauliflower extract within 5 minutes；
Second step adds in sealing high steam pot after fresh bamboo shoot tip is cleaned up, then pass to 500 DEG C of steam
Boiling 10 minutes is carried out, then by cool to water is dried in room temperature, gravity-flow ventilation, 40 DEG C of dryer of addition carries out being dried to moisture content
15-20%；
Third walks, and with the material obtained after the processing of first and second step after Bunge Corydalis Herb, timothy grass are crushed, is subsequently added into mixing
Beneficial bacterium is carried out after building heap fermentation 8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned Section III mixed fodder, diseases prevention auxiliary liquid are prepared by following raw material：Belladonna extract
50mg/l, henbane extract 50mg/l, vanilla extract 40mg/l, common cnidium fruit P.E 80mg/l, raspberry
Extract 85mg/l, Gentrin Knotweed P.E 95mg/l, Folium Artemisiae Argyi extract 60mg/l, galangal rhizome extract 45mg/l；
In the diseases prevention auxiliary liquid, belladonna extract is prepared by following methods：Belladonna is cleaned into crushing, is added in
55% (v/v) ethanol solution carries out heating and refluxing extraction 15 minutes at 55 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor,
The acetum for being subsequently added into 7 times of volumes adjusts pH to 5, at 55 DEG C, 110 hertz of high-voltage pulse electric field pulse processing 2.5 ×
10-6S is extracted 25 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure,
Spray drying can obtain belladonna extract, and master contains alkaloid 46.251mg/g in extract；
The henbane extract is prepared by following methods：Suitable diatomite is added in after henbane seed is cleaned
It is slurry to carry out mortar grinder, is subsequently added into 60% (v/v) ethanol solution and heating and refluxing extraction is carried out at 45 DEG C 7 minutes, filters
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 4.5 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 25 minutes at 48 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can obtain henbane extract, and master contains total alkaloid 56.24mg/g in extract.
The vanilla extract is prepared by following methods：After vanilla is carried out ultramicro grinding under pressure 4MPa
15 mesh sieve is crossed, 15 times of water refluxing extractions of addition are extracted 25 minutes at 55 DEG C obtains extracting solution, then adds in extracting liquid volume 1.3
Petroleum ether again is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, it is molten to be subsequently added into 82% (v/v) ethyl alcohol
Liquid carries out alcohol extracting 1.5 hours, then carries out adsorbing separation elution with macroreticular resin, elution is successively 20% with volumetric concentration,
40%th, 60%, 80% and 90% ethanol-water system eluent carries out gradient elution, the ethanol-water solution elution of each gradient
Volume is 5.5BV, elution flow rate 3.5BV/h, is concentrated under reduced pressure to give paste, dry to can obtain vanilla extract, is extracted
It is main in object to contain coumaric acid glycosides 51.23mg/g；
The common cnidium fruit P.E is prepared by following methods：White oil, diatomite are added in after frutus cnidii is cleaned
It grinds slurry, then adds in 82% (v/v) ethanol solution refluxing extraction 3 times, the temperature extracted every time is 65 DEG C, the time of extraction
It it is 35 minutes, the extracting solutions of 3 extractions of merging add in filtering removal activated carbon after activated carbon decolorizing, add after being then diluted with water
Enter after n-butanol oscillation is rocked and stand 7 minutes, then n-butanol fraction is taken to be detached with silica gel column chromatography, with petroleum ether-acetic acid second
Ester carries out gradient elution for eluant, eluent, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give common cnidium fruit P.E master containing
There is Osthole 25.31mg/g；The frutus cnidii：White oil：Diatomaceous weight ratio is 2:0.06：0.2.
The Fructus Rubi extract is prepared by following methods：Raspberry drying is surpassed under pressure 4MPa
80 mesh sieve is crossed after Crushing of Ultrafine, impregnating raspberry tea powder using 4 times of volumes, the water termostat that temperature is 110 DEG C obtains soup tea in 8 minutes,
Vinegar acid for adjusting pH is subsequently added into 2.5, extracting solution is taken using 70% (v/v) ethanol solution refluxing extraction, then according to volume ratio
It is 1:1 ratio adds in ethyl acetate and carries out standing extraction after swaying 25 minutes, by ethyl acetate portion silica gel column chromatography point
From using petroleum ether-ethyl acetate as eluant, eluent progress gradient elution, 40：1 elution fraction carries out 5 times with acetone and recrystallizes
Contain Quercetin 25.36mg/g to Fructus Rubi extract master；
The Gentrin Knotweed P.E is prepared by following methods：After giant knotweed is crushed, it is molten to add in 82% (v/v) ethyl alcohol
Liquid carries out refluxing extraction 35 minutes under ultrasonic wave subsidiary conditions at 85 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain Gentrin Knotweed P.E master and contain resveratrol
27.31mg/g。
The galangal rhizome extract is prepared by following methods：Galangal is added in 15 times of water and decocts 55 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.03 times of galangal rhizome extracts that can obtain galangal active constituent of original volume；
Folium Artemisiae Argyi extract is prepared by following methods：Folium artemisiae argyi is added in 18 times of water to decoct 55 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into 1.04 times of Folium Artemisiae Argyi extracts that can obtain folium artemisiae argyi active constituent of original volume.
Embodiment 3：
A kind of cultural method of high-quality longevity perfume (or spice) chicken, specifically includes following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses
The solarization time reaches the hillside of 7 hours as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse on the hillside of 800 meters of water resource or more
The solarization time reaches 7 hours, 90 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, and every longevity put in a suitable place to breed is fragrant
Chicken density is 1200,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 4 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, hatched
Longevity phase perfume (or spice) egg is to be positioned on the rice straw after disinfection with nest, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period with 5
Between keep the coop ventilation amount to be 0.4m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse, morning 9 next day:00, which opens henhouse Men Youqi, puts life in a suitable place to breed,
The first time free-ranging since the third day after longevity perfume (or spice) chicken hatches starts with the tinkle of bells training longevity perfume (or spice) chicken from free-ranging in first day and goes out
Column, the time into column, and send out alarm training longevity perfume (or spice) chicken go back to hillside at noon and eat feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination stream
Dynamic mountain spring water starts to second month feeding I mixed fodder after fed with water for 3.5 hours, injected volume for 150g/ only；Second
Start within a month to four month feeding Section II mixed fodder, injected volume is 250g/, when longevity perfume (or spice) chicken was at four month to the 7th
Month feeding Section III mixed fodder, injected volume for 250g/ only；
The I mixed fodder is mixed to get by following raw material：30 parts by weight of soy meal, 40 parts by weight of corn flour, chicken intestines
20 parts by weight of powder, 5 parts by weight of amino acid, 0.6 parts by weight of salt, 0.6 parts by weight of sodium bicarbonate, 0.4 parts by weight of fiery sesame oil；
The Section II mixed fodder is prepared by following raw material：20 parts by weight of soy meal, 14 parts by weight of corn flour, chicken intestines
14 parts by weight of powder, 5 parts by weight of amino acid, 1.5 parts by weight of salt, 0.8 parts by weight of sodium bicarbonate, 0.4 parts by weight of fiery sesame oil, pumpkin
10 parts by weight of skin, 10 parts by weight of hayashishita weeds, 7 parts by weight of mantis；
The Section III mixed fodder is prepared by following raw material：18 parts by weight of Cottonseed Meal, 13 parts by weight of husk, bone meal
15 parts by weight, 6 parts by weight of amino acid, 0.8 parts by weight of salt, 0.5 parts by weight of sodium bicarbonate, 8 parts by weight of yellow meal worm, 10 weight of fermentation material
Measure part, diseases prevention auxiliary liquid 5.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed when five month big with addition of health care, injected volume is
Only, the health care addition feed is prepared 25g/ by following raw material：22 parts by weight of the fleece-flower root, 2 parts by weight of Russula delica, Caulis Spatholobi
1.1 parts by weight, 0.6 parts by weight of mulberry leaf powder；
(6) it delivers for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
One of raw material fermentation material is prepared by following raw material in above-mentioned Section III mixed fodder：21 parts by weight of timothy grass,
16 parts by weight of fourth grass, 12 parts by weight of bamboo shoot tip, 13 parts by weight of Cauliflower extract, mixing 5 parts by weight of beneficial bacterium are (by following raw material
It is mixed to get：3 parts by weight of cellulase that viable count is 1,100,000,000 cfu/g, 2 weight of bacillus subtilis that viable count is 800,000,000 cfu/g
Measure part, 2 parts by weight of pichia that viable count is 500,000,000 cfu/g, 2 weight of bifidobacterium pseudocatenulatum that viable count is 700,000,000 cfu/g
Measure part)；
One of raw material fermentation material is prepared by following methods in above-mentioned Section III mixed fodder：
The water for selecting 4 times of volumes of good Cauliflower addition is blended mashing and is fitted into conical flask for 12 minutes, connect by the first step
The sodium bicarbonate solution adjusting pH to 5.5 for 0.06% (w/v) of addition obtains mixed serum, adds 2.5% volume of slurries
By 8 parts by weight subtilopeptidase As, 6 parts by weight bromelains, 6 parts by weight ficins, 5 parts by weight Papains
The mixing protease of enzyme composition carries out the shaking table that rate is 90r/min in conical flask and digests 17 minutes at 52 DEG C, cross 150 mesh sieve
Afterwards, centrifugal rotational speed is used to carry out centrifugation for 2200r/min centrifuges and can obtain Cauliflower extract within 4 minutes；
Second step adds in sealing high steam pot after fresh bamboo shoot tip is cleaned up, then pass to 400 DEG C of steam
Boiling 6 minutes is carried out, then by cool to water is dried in room temperature, gravity-flow ventilation, 39 DEG C of dryer of addition carries out being dried to moisture content
17%；
Third walks, and with the material obtained after the processing of first and second step after Bunge Corydalis Herb, timothy grass are crushed, is subsequently added into mixing
Beneficial bacterium is carried out after building heap fermentation 7 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
One of raw material in above-mentioned Section III mixed fodder, diseases prevention auxiliary liquid are prepared by following raw material：Belladonna extract
60mg/l, henbane extract 60mg/l, vanilla extract 70mg/l, common cnidium fruit P.E 90mg/l, raspberry
Extract 105mg/l, Gentrin Knotweed P.E 115mg/l, Folium Artemisiae Argyi extract 70mg/l, galangal rhizome extract 50mg/l；
In the diseases prevention auxiliary liquid, belladonna extract is prepared by following methods：Belladonna is cleaned into crushing, is added in
65% (v/v) ethanol solution carries out heating and refluxing extraction 20 minutes at 60 DEG C, and extracting solution is taken to be concentrated under reduced pressure up to no ethanol flavor,
The acetum for being subsequently added into 10 times of volumes adjusts pH to 5.5, at 55 DEG C, 120 hertz of high-voltage pulse electric field pulse processing 3 ×
10-6S is extracted 30 minutes, is obtained crude extract, then carry out layering extraction using ethyl acetate, and ethyl acetate portion is taken to be concentrated under reduced pressure,
Spray drying can obtain belladonna extract, and master contains alkaloid 40.15mg/g in extract；
The henbane extract is prepared by following methods：Suitable diatomite is added in after henbane seed is cleaned
It is slurry to carry out mortar grinder, is subsequently added into 65% (v/v) ethanol solution and heating and refluxing extraction is carried out at 50 DEG C 10 minutes, filters
Filtrate is taken, filtrate is recycled into ethyl alcohol up to no alcohol taste by reduced pressure, is subsequently added into after dilute hydrochloric acid adjusts pH to 5.0 and filters to take
Supernatant is subsequently added into cellulase water enzyme digestion 30 minutes at 50 DEG C, is subsequently added into sodium hydroxide and adjusts pH to neutrality, mistake
Leaching precipitation can obtain henbane extract, and master contains total alkaloid 48.25mg/g in extract.
The vanilla extract is prepared by following methods：After vanilla is carried out ultramicro grinding under pressure 5MPa
20 mesh sieve is crossed, 20 times of water refluxing extractions of addition are extracted 30 minutes at 60 DEG C obtains extracting solution, then adds in extracting liquid volume 1.5
Petroleum ether again is extracted, and petroleum ether portion is taken then to recycle petroleum ether with water-bath distillation, it is molten to be subsequently added into 85% (v/v) ethyl alcohol
Liquid carries out alcohol extracting 2 hours, then carries out adsorbing separation elution with macroreticular resin, elution is successively 20% with volumetric concentration, 40%,
60%th, 80% and 90% ethanol-water system eluent carries out gradient elution, the ethanol-water solution elution volume of each gradient
For 6BV, elution flow rate 4BV/h, paste is concentrated under reduced pressure to give, dry to can obtain vanilla extract, master contains in extract
There is coumaric acid glycosides 46.28mg/g；
The common cnidium fruit P.E is prepared by following methods：White oil, diatomite are added in after frutus cnidii is cleaned
Grind slurry, then add in 85% (v/v) ethanol solution refluxing extraction 3 times, the temperature extracted every time for DEG C, time of extraction be
40 minutes, merge the extracting solution of 3 extractions, add in filtering removal activated carbon after activated carbon decolorizing, added in after being then diluted with water
N-butanol oscillation stands 10 minutes after rocking, and then n-butanol fraction is taken to be detached with silica gel column chromatography, with petroleum ether-ethyl acetate
Gradient elution is carried out for eluant, eluent, 40：1 elution fraction, carries out 5 times with acetone and is recrystallized to give common cnidium fruit P.E master containing
Osthole 20.24mg/g；The frutus cnidii：White oil：Diatomaceous weight ratio is 3:0.07：0.2.
The Fructus Rubi extract is prepared by following methods：Raspberry drying is surpassed under pressure 5MPa
It is sieved with 100 mesh sieve after Crushing of Ultrafine, impregnating raspberry tea powder using 5 times of volumes, the water termostat that temperature is 120 DEG C obtains soup in 10 minutes
Tea is subsequently added into vinegar acid for adjusting pH to 3.0, extracting solution is taken using 75% (v/v) ethanol solution refluxing extraction, then according to volume
Than being 1:1 ratio adds in ethyl acetate and carries out standing extraction after swaying 30 minutes, by ethyl acetate portion silica gel column chromatography
Separation carries out gradient elution, 40 by eluant, eluent of petroleum ether-ethyl acetate：1 elution fraction carries out 5 recrystallizations with acetone
It obtains Fructus Rubi extract master and contains Quercetin 18.25mg/g；
The Gentrin Knotweed P.E is prepared by following methods：After giant knotweed is crushed, it is molten to add in 85% (v/v) ethyl alcohol
Liquid carries out refluxing extraction 40 minutes under ultrasonic wave subsidiary conditions at 90 DEG C, and refluxing extraction 3 times altogether merge No. 3 extracting solutions
It is concentrated under reduced pressure, low temperature is evaporated to obtain the medicinal extract of 1.12 times of original volume, you can obtain Gentrin Knotweed P.E master and contain resveratrol
22.14mg/g。
The galangal rhizome extract is prepared by following methods：Galangal is added in 20 times of water and decocts 60 points
Clock, filters to take filtrate, and reduction vaporization is concentrated into 1.05 times of galangal rhizome extracts that can obtain galangal active constituent of original volume；
Folium Artemisiae Argyi extract is prepared by following methods：Folium artemisiae argyi is added in 20 times of water to decoct 60 minutes, is filtered to take
Filtrate, reduction vaporization are concentrated into 1.05 times of Folium Artemisiae Argyi extracts that can obtain folium artemisiae argyi active constituent of original volume.
Experiment one：
Comparative example 1：The preparation method of fermentation material：The first step, it is same as Example 1；Second step, by Bunge Corydalis Herb, bamboo shoot tip,
The material that timothy grass obtains after being handled after crushing with the first step is subsequently added into mixing beneficial bacterium and carries out after building heap fermentation 5 days,
The center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
Comparative example 2：The preparation method of fermentation material：Fresh soybean complete stool is cleaned up and carries out water and carry, concentrate by the first step
Obtain Cauliflower extract；Second step, it is same as Example 1；Third walk, by Bunge Corydalis Herb, timothy grass crush after with first and second step
The material obtained after processing is subsequently added into mixing beneficial bacterium and carries out after building heap fermentation 5 days, and fermentation heap is controlled during fermentation
Center is not more than 55 DEG C.
Comparative example 3：The preparation method of fermentation material：The first step, it is same as Example 1；Second step, it is same as Example 1；The
Three steps are carried out with the material obtained after the processing of first and second step after building heap fermentation 5 days, hair after crushing Bunge Corydalis Herb, timothy grass
The center of fermentation heap is controlled to be not more than 55 DEG C during ferment.
Comparative example 4：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, bacillus subtilis, viable count are identical with parts by weight；
Comparative example 5：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, pichia, viable count are identical with parts by weight；
Comparative example 6：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, bifidobacterium pseudocatenulatum, viable count are identical with parts by weight；
Comparative example 7：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Bacillus subtilis, pichia, bifidobacterium pseudocatenulatum, viable count are identical with parts by weight；
Comparative example 8：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, bacillus subtilis, bifidobacterium pseudocatenulatum, viable count are identical with parts by weight；
Comparative example 9：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, pichia, bifidobacterium pseudocatenulatum, viable count are identical with parts by weight；
Comparative example 10：The preparation method of fermentation material is substantially same as Example 1, and difference is mixing beneficial bacterium by following original
Material is mixed to get：Cellulase, bacillus subtilis, pichia, viable count are identical with parts by weight；
Embodiment 1-3, control group 1-10 modes treated fermentation material are subjected to CN-Residual concentration detects, according to fermentation
Cyanogen rate drops in being calculated containing cyanometer for fermentation material before and after the processing, wherein, drop cyanogen rate %=(concentration containing cyanogen-reactions in reaction primary fermentation material
Concentration containing cyanogen in fermentation material afterwards)/concentration containing cyanogen in primary fermentation material is reacted, and Parallel testing three times is done in detection every time, is averaged
Value.
Table 1
As seen from the above table, cyanogen rate drops in comparative example 1 compared with Example 1 reduces 30.3%, illustrates to carry out bamboo shoot tip special
The content of phase generation cyanide after fermentation can be reduced after different processing, the as toxin of bamboo shoot tip in itself will can be obtained before fermentation
To removing, the expection before this imagines with experiment is consistent.Relatively drop cyanogen rate reduces 19.3% to comparative example 2 compared with Example 1, card
Understand the content higher containing dynamic element (sugar, protein) in the Cauliflower extract Jing Guo special extraction, provided in the fermentation phase
One good growing environment of zymophyte can give full play to the capacity of decomposition of flora；Cyanogen relatively drops in comparative example 3 compared with Example 1
Rate reduction knows clearly 53.8%, it was demonstrated that has good capacity of decomposition between mixing beneficial bacterium, then in order to verify this viewpoint, does
The contrast test of comparative example 4-10, the single fermentation bacterium solution of comparative example 4-6 reduce 20% left side compared to embodiment 1-3 drop cyanogen abilities
It is right；, comparative example 8-10 amount amount mixed bacteria liquid cyanogen ability dropped compared with embodiment 1-3 reduce 16% or so；It can be seen that
The ability that there is mutually collaboration of complementing each other to drop cyanogen using the combined fermentation bacterium of the application, promotes drop cyanogen ability to greatly improve.
Experiment two：
Using diseases prevention auxiliary liquid prepared by the embodiment of the present invention 1 to the emergency toxicology experimental observation of longevity perfume (or spice) chicken
Experiment carries out in the clinical labororatory of ecological agriculture development Co., Ltd of Bama of Guangxi Green City.Experiment longevity perfume (or spice) chicken is purchased from
Hechi City Jin Cheng Jiang Lao streets wholesale market, 1 kilogram or so of specification, experiment set 4 concentration gradient medicine groups altogether using enema
(1.0g/l, 2.0g/l, 5.0g/l, 10.0g/l) and 1 blank control group for being not added with drug, every group sets 3 repetitions, each group
Longevity perfume (or spice) chicken 20 is put, is specifically shown in Table 1.It after on-test, is observed continuously, records longevity perfume (or spice) chicken exception and death condition.
The emergency toxicology experiment grouping of 1 pair of longevity perfume (or spice) chicken
2 different pharmaceutical concentration lower longevity of table perfume (or spice) chicken death situation
It the results are shown in Table 2.Control group and 1.0g/l drug concentration groups find death, and the normal 1.0g/ of activity without longevity perfume (or spice) chicken
L groups, average mortality reaches 10.0%, 5.0g/l groups after 96h, adds up average mortality 23.33%, and 10.0g/l groups add up flat
The equal death rate 55.0%.
It is LD50=0.34745ml/m to longevity perfume (or spice) chicken half lethal dose to calculate the embodiment of the present invention 1 with Bliss methods3,
Regression equation is y (Probit)=6.438+3.1321Log (D), is specifically shown in Table 3.By Te Lunbao formula (safe concentrations
=48hLC50*0.3/ (LC/48hLC50 for 24 hours)) calculate the application liquid insecticide it is dense to the safety of longevity perfume (or spice) chicken
It spends and is
0.0955ml/m3, illustrate that the embodiment of the present invention 1 is higher to the safety of longevity perfume (or spice) chicken.
Table 3Bliss methods calculate the embodiment of the present invention 1 to longevity perfume (or spice) chicken median lethal dose
Experiment three：
1.1 experiments kind
The longevity perfume (or spice) chicken (500 ± 10g of weight) of severe infections global-worm illness is purchased from Hechi City Jin Cheng Jiang Lao streets.Selection has spirit
Bad, feather towers, head is crispaturaed, stand still for a long while an a corner, be off one's feed, cockscomb and visual mucous membrane anaemia, pale, gradual assistant, excrement
The longevity perfume (or spice) chicken 1100 of appearance coccidia disease symptoms into water sample and with blood, is randomly assigned to be 11 groups, every group of 100 longevity perfume (or spice)
One henhouse of chicken.
Experimental group 1-3 assists liquid bowel lavage using the diseases prevention that embodiment 1-3 is prepared respectively, and bowel lavage was primary on 1st, every time
20 milliliters；
Control group 1-8 contains belladonna extract, henbane seed extraction, vanilla extract, frutus cnidii using only single respectively
Extract, Fructus Rubi extract, Gentrin Knotweed P.E, galangal rhizome extract, Folium Artemisiae Argyi extract anti-disease substance launch into henhouse, one
Day bowel lavage is primary, 20 milliliters every time.
The death rate of observation longevity perfume (or spice) chicken was taken after 2 weeks,
Longevity perfume (or spice) chicken average mortality (%)=(longevity perfume (or spice) chicken amount of survival during longevity perfume (or spice) chicken quantity-microscopy of dispensing)/dispensing
The sample highest effective concentration of longevity perfume (or spice) chicken quantity × 100%, i.e., the solvent extractable matter concentration corresponding to highest killing rate.
4 each groups of medicaments of table are to the control effect table of global-worm illness
Result of the test shows：The epidemic disease of the global-worm illness of longevity perfume (or spice) chicken is prevented with excellent using the diseases prevention auxiliary liquid of the application
Effect is controlled, the extract for the control group 1-8 single uses that compare, which compares, not only has the effect of higher prevention global-worm illness, and
Insecticidal effect is more preferably lasting, it was demonstrated that the formula of the application has the function of mutually to assist collaboration.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of cultural method of high-quality longevity perfume (or spice) chicken, which is characterized in that specifically include following steps：
(1) addressing：Selection has free of contamination water resource, possesses 6 years raw more than pine trees and the woods of miscellaneous tree miscegenation, possesses solarization
Time reaches the hillside of 6-8 hours as farm；
(2) henhouse construction：It selects building several henhouses, every henhouse solarization on the hillside of 800 meters of water resource or more
Time reaches 6-8 hours, 80-100 square metres of the area of every henhouse, and henhouse spacing reaches 800 meters or more, every longevity put in a suitable place to breed
Fragrant chicken density is 1000-1500,
(3) hatch：Longevity perfume (or spice) egg is put into after hatching 3-5 days in incubator and taken out, continue to hatch to longevity perfume (or spice) chicken hen, the incubation period
Longevity perfume (or spice) egg is to be positioned on the rice straw after disinfection with nest, and longevity perfume (or spice) chicken hen of leaving freely is cooked nest, incubation period with 4-6
Between keep the coop ventilation amount to be 0.2-0.5m3/ s, until hatching chicken；
(4) aquaculture management：Longevity perfume (or spice) chicken is cultivated in a manner that free-ranging is with addition of stable breeding；
Its free choice feeding is given, afternoon 17:00, which calls out chicken, returns henhouse first time free-ranging since the third day after longevity perfume (or spice) chicken hatches, from
Free-ranging in first day start with the tinkle of bells training longevity perfume (or spice) chicken deliver for sale, the time into column, and it is fragrant to send out the alarm training longevity at noon
Chicken goes back to hillside and eats feed；
Start feeding after 48 hrs when hatching chicken recoil, first fed with water before feeding, water resource is free of contamination flowing mountain
Spring, the 3-4 hours after fed with water start to second month feeding I mixed fodder, injected volume for 100-200g/ only；Second
Start within a month to four month feeding Section II mixed fodder, injected volume for 200-300g/ only, when longevity perfume (or spice) chicken was at four month to the
Seven months feeding Section III mixed fodders, injected volume are 200-300g/；
The I mixed fodder is mixed to get by following raw material：Soy meal 20-40 parts by weight, corn flour 30-50 parts by weight, chicken
Rice noodle roll end 10-30 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight, fire fiber crops
Oily 0.1-0.6 parts by weight；
The Section II mixed fodder is prepared by following raw material：Soy meal 15-30 parts by weight, corn flour 10-20 parts by weight, chicken
Rice noodle roll end 10-18 parts by weight, amino acid 3-8 parts by weight, salt 1-2 parts by weight, sodium bicarbonate 0.5-1.0 parts by weight, fiery sesame oil
0.2-0.6 parts by weight, pumpkin peel 5-15 parts by weight, hayashishita weeds 5-15 parts by weight, mantis 3-10 parts by weight；
The Section III mixed fodder is prepared by following raw material：Cottonseed Meal 10-25 parts by weight, husk 10-20 parts by weight, bone
Powder 10-20 parts by weight, amino acid 3-8 parts by weight, salt 0.5-1.0 parts by weight, sodium bicarbonate 0.3-0.8 parts by weight, yellow meal worm 5-
10 parts by weight, fermentation material 5-15 parts by weight, diseases prevention auxiliary liquid 1.0-10.0g/l；
(5) health care cultivation is handled：When longevity perfume (or spice) chicken also additionally adds feed, injected volume 20- when five month big with addition of health care
Only, the health care addition feed is prepared 30g/ by following raw material：Fleece-flower root 15-28 parts by weight, Russula delica 1-3 parts by weight,
Caulis Spatholobi 0.8-1.5 parts by weight, mulberry leaf powder 0.3-0.8 parts by weight；
(6) it delivers for sale：Longevity perfume (or spice) chicken is delivered for sale dealing after health care addition feed 3 months is fed.
2. the cultural method of high-quality longevity perfume (or spice) chicken as described in claim 1, it is characterised in that：The fermentation material is by following raw material
It is prepared：Timothy grass 15-25 parts by weight, Bunge Corydalis Herb 10-20 parts by weight, bamboo shoot tip 8-15 parts by weight, Cauliflower extract 5-
20 parts by weight, mixing beneficial bacterium 3-8 parts by weight.
3. the cultural method of high-quality longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The mixing beneficial bacterium is by following
Raw material is mixed to get：Cellulase 3-8 parts by weight, bacillus subtilis 2-6 parts by weight, pichia 2-4 parts by weight, false chain
Shape Bifidobacterium 2-5 parts by weight.
4. the cultural method of high-quality longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The viable count of the cellulase
Viable count for hundred million cfu/g of 5-15, bacillus subtilis is hundred million cfu/g of 5-10, the viable count of pichia is hundred million cfu/ of 3-8
G, the viable count of bifidobacterium pseudocatenulatum is hundred million cfu/g of 5-10.
5. the cultural method of high-quality longevity perfume (or spice) chicken as claimed in claim 2, it is characterised in that：The fermentation material is by with lower section
What method was prepared：
The water for selecting 3-6 times of volume of good Cauliflower addition is blended mashing and is fitted into conical flask for 10-15 minutes by the first step,
The sodium bicarbonate solution adjusting pH to 5-6 for being subsequently added into 0.05-0.08% (w/v) obtains mixed serum, adds slurries 2-3%
The mixing protease of volume carries out shaking table in conical flask and digests 15-20 minutes at 50-55 DEG C, after crossing 150 mesh sieve, using centrifugation
Machine carries out centrifugation and can obtain Cauliflower extract in 2-5 minutes；
Second step adds in sealing high steam pot after fresh bamboo shoot tip is cleaned up, then pass to 300-500 DEG C of steam
Boiling 5-10 minutes is carried out, then by cool to water is dried in room temperature, gravity-flow ventilation, 38-40 DEG C of dryer of addition be dried to containing
Water rate 15-20%；
Third walks, and with the material obtained after the processing of first and second step after Bunge Corydalis Herb, timothy grass are crushed, it is beneficial to be subsequently added into mixing
Bacterium is carried out after building heap fermentation 5-8 days, and the center of fermentation heap is controlled to be not more than 55 DEG C during fermentation.
6. the cultural method of high-quality longevity perfume (or spice) chicken as claimed in claim 5, it is characterised in that：The mixing protease is by 3-
8 parts by weight subtilopeptidase As, 3-6 parts by weight bromelain, 2-6 parts by weight ficin, 1-5 parts by weight pawpaws
Protease forms.
7. the cultural method of high-quality longevity perfume (or spice) chicken as claimed in claim 5, it is characterised in that：The rate of the shaking table is 80-
100r/min, the centrifugal rotational speed of the centrifuge is 2000-2500r/min.
8. the cultural method of high-quality longevity perfume (or spice) chicken as described in claim 1, it is characterised in that：The diseases prevention assists liquid by following
Raw material is prepared：Belladonna extract 30-60mg/l, henbane extract 30-60mg/l, vanilla extract 30-70mg/l, snake
Machine tool extract 70-90mg/l, Fructus Rubi extract 65-105mg/l, Gentrin Knotweed P.E 80-115mg/l, Folium Artemisiae Argyi extract 50-
70mg/l, galangal rhizome extract 40-50mg/l.
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