CN108057051B

CN108057051B - Composition containing saussurea involucrate and cubilose extract and preparation method and application thereof

Info

Publication number: CN108057051B
Application number: CN201610980454.9A
Authority: CN
Inventors: 张艳芳; 张炜铤; 詹华强; 董婷霞; 陈嘉伦; 黄振辉; 吴启韵; 谭天乐
Original assignee: Hoi Wan Trading Co ltd
Current assignee: Huizhou Mianli Biotechnology Co ltd
Priority date: 2016-11-08
Filing date: 2016-11-08
Publication date: 2021-11-23
Anticipated expiration: 2036-11-08
Also published as: CN108057051A

Abstract

The invention relates to a composition containing saussurea involucrate extract and cubilose extract, related application and product thereof, and a preparation method. The invention can fully exert the effects of the saussurea involucrate and the cubilose, and has obviously enhanced oxidation resistance and whitening effect. The preparation process is simple, the cost can be reduced, and increasingly deficient natural precious resources of the wild saussurea involucrate can be protected.

Description

Composition containing saussurea involucrate and cubilose extract and preparation method and application thereof

Technical Field

The invention relates to a product for disease treatment, health care or beauty treatment, in particular to application of saussurea involucrate and cubilose extract in the product.

Background

Saussurea involucrate, also known as Saussurea involucrate flower (kar. et Kir.) Sch. -Bip.), is shaped like lotus flower, so it is called Saussurea involucrate. Is perennial herb and is 15-35 cm high. The rhizome is thick and the neck is left with most of the brown leaves. Thick and strong stem without hair. Dense leaves, basal leaves and cauline leaves without stems, oval leaves or oval ovate leaves; the most upper part is in the shape of leaf bud, membranous and light yellow, and surrounds the general inflorescence, and sharp teeth are arranged on the edge. 10-20 head inflorescences, and spherical total inflorescences are densely arranged at the top of the stem. The involucre is hemispherical and has the diameter of 1 cm; 3-4 layers of bract, purple brown at edge or all. The floret is purple. The slim fruit is oblong. The crown hair is white. The flower and fruit period is 7-9 months. It is distributed in the alpine region of many places in China, and Russia and Kazakhstan are also distributed. The sea surface is grown in rock cracks, stone walls and gravel beaches near the snow lines of the mountains, and the altitude is 2400 + 4000 meters. Tianshan produced the most and the best quality. Can be used as a medicine and also has certain ornamental value. In 1996, the saussurea involucrate has been listed as a secondary protective plant in China, is the only saussurea involucrate plant listed in the book of Chinese plant Red skin, and is a third-level endangered species in China. The No. 13 document of the State Council of 2000 has made the prohibition of collecting and digging wild saussurea involucrate. The wild saussurea involucrate is bred by seeds, the growth period needs 6 to 8 years, and if the root of the wild saussurea involucrate is pulled out, the saussurea involucrate can not grow in place. In the fifth and sixty years of the last century, saussurea involucrate can be collected on gravel slope with altitude of about 1800 m. The area of the saussurea involucrate in China is about 5000 ten thousand mu, but the area is less than 1000 ten thousand mu at present. 7-8 months per year is the full-blown season of wild snow lotus herb in Tianshan mountain in Xinjiang, however, every time, a large number of lawbreakers come to wild in the front and are harvested in the vicinity of a snow line of more than 3000 meters. Local experts have shown over and over again that if this phenomenon is not contained, it is not for years possible for this precious species saussurea involucrata to disappear from the earth.

Snow lotus herb contains protein, amino acid, flavonoid, alkaloid, etc. Has effects in dredging channels, promoting blood circulation, expelling cold, removing dampness, stopping bleeding, relieving swelling, and removing toxic materials. Snow lotus herb is warm in nature and sweet and bitter in taste, enters liver, spleen and kidney meridians, has the functions of dispelling cold, tonifying yang, regulating menstruation and stopping bleeding, and is used for treating impotence, weak waist and knees, irregular menstruation, metrorrhagia and metrostaxis, leukorrhagia, rheumatic arthritis, traumatic hemorrhage and other diseases. In the book of Ben Cao gang mu Shi Yi of the Ming Dynasty Zhao academic Ming, there are records of "snow on the cold land, spring and summer are not scattered, there is grass in the snow, similar to the Du stem of lotus, Ting Xue Yuan ai" and "there are Tianshan in the land, snow on the winter and summer, there are lotus in the snow, and the one on the top of Tianshan mountain is the first. Nature and taste: bitter, slightly bitter and warm; the functional indications are as follows: clearing away heat and toxic material, dispelling pathogenic wind and dampness, relieving swelling and pain. The compendium of materia Medica is recorded in Shi Yi (supplement to compendium of materia Medica): the shape of snow lotus herb is similar to that of lotus flower, and the position of yili is the first one to produce Tianshan crest. It correctly describes the shape and origin of saussurea involucrate. Saussurea involucrate, also known as saussurea involucrate and Chinese pholidota herb, is a perennial herb of the compositae family, is grown in rock seams, stone walls and gravel beaches near snow lines of Tianshan mountain, Altai mountain and Kunlun mountain, and produces the most and the best quality from Tianshan. In the aspect of beautifying, the skin care product can accelerate the metabolism of the skin, reduce wrinkles and keep the skin glossy and plump. Delaying senility. And has good therapeutic effect on facial freckle and chloasma hepaticum. In addition, the natural saussurea involucrate contains natural saussurea involucrate flavonoid reed, saussurea involucrate lactone, saussurea involucrate polysaccharide and the like. The sun protection index of the sun protection material is as high as 22 DSE. Therefore, the skin can be protected from being damaged by ultraviolet rays, the skin pigmentation can be improved, the aging of a human body can be delayed, and people can keep youth.

The nidus Collocaliae is a nest formed by mixing secreted saliva or saliva and its down feather, and coagulating on cliff. The main producing areas are Indonesia, Malaysia, Vietnam and the like, and according to the record of 'materia medica Shi Yi': bird's nest is sweet and mild, and can nourish lung yin, resolve phlegm and stop cough, tonify and clear, so it is a holy drug for conditioning consumptive disease, all diseases can be treated by lung deficiency and can not clear and descend. The traditional Chinese medicine considers that the bird's nest has the effects of nourishing yin and moistening dryness, tonifying qi and strengthening the middle warmer, and is suitable for patients with weak constitution, malnutrition, chronic dysentery, chronic malaria, excessive phlegm and cough, old chronic bronchitis, bronchiectasis, emphysema, pulmonary tuberculosis, hemoptysis, hematemesis and stomachache to eat. Modern medicine finds that the bird's nest can promote the immunologic function and has the effects of delaying the aging of a human body and prolonging the life. The effect of cubilose on improving skin mainly comes from the theory of traditional Chinese medicine. Qing Dynasty famous physicians Zhang Luo in Ben Jing Yuan (the origin of the menstrual cycle), described the bird's nest "capable of promoting the generation of gold and water, nourishing the lung with kidney qi, and regulating stomach qi. In addition, in Huangdi's classic "lung governs skin hair and governs breathing", it can be seen that the effect of bird's nest on improving skin is mainly through improving lung function.

The occurrence of many diseases has been shown to be associated with free radicals in recent years. Therefore, the use of antioxidant mechanisms to treat many diseases including cardiovascular diseases, chronic nephritis, Alzheimer's disease, and allergic diseases has been increasingly appreciated. Natural products with antioxidant capacity are more widely used in cosmetics for anti-skin aging.

Melanin on the human skin is transformed from dopachrome produced by Melanocytes (Melanocytes). Melanin gradually matures in melanosomes (melanosomes) within melanocytes, and after maturation, melanosomes are transferred to Keratinocytes (Keratinocytes) along the dendrites of melanocytes, resulting in darker skin color. Inhibiting tyrosinase activity can effectively reduce the production of dopachrome and melanin, thereby enabling skin color to become lighter and whiter. Most of the existing whitening drugs such as Hydroquinone (Hydroquinone) and Arbutin (Arbutin) achieve the whitening effect by aiming at the activity of tyrosinase. Although hydroquinone has an obvious whitening effect, it was forbidden in 2006 because of the risk of carcinogenesis. Thereafter, arbutin is also forbidden because of its chemical structure similar to hydroquinone. The current scientific community is looking for whitening products with higher safety to replace hydroquinone and the like.

At present, a plurality of cosmetic products prepared from natural plant extracts exist in the market, but the antioxidant or whitening effect is not obvious, or the ideal antioxidant and whitening effect is achieved at the cost of complex process, high cost and the like.

In addition, different regions have different traditional and cultural medicines. With the development of thousands of years of traditional Chinese medicines, different areas gradually form characteristic traditional and culture of medicines, wherein the wild of the traditional Chinese area (northern area) and the Lingnan area (southern area) is the most obvious. For example, the growing environment of Tianshan saussurea involucrate is far from that of edible bird's nest. The saussurea involucrate grows in the extremely cold environment outside the northeast of the desert and at the peak of the mountain; the edible bird's nest is produced in a dark cave in the Michelson forest in each island on the equator. From ancient times to present, compatibility of north and south crosses such as saussurea involucrate and cubilose is very rare in traditional Chinese medicine formulas.

Disclosure of Invention

The first purpose of the invention is to provide a composition, which comprises saussurea involucrate extract and cubilose extract.

In one aspect, the saussurea involucrate extract can be aqueous or alcoholic saussurea involucrate extract. In another aspect, the bird's nest extract may be a bird's nest water extract.

In one aspect, the weight ratio of the saussurea involucrate extract to the bird's nest extract is in the range of about 5:1 to 1:2, preferably about 5:1 to 1:1, for example about 5:1, 4.5:1, 4:1, 3.5:1, 3.4:1, 3.3:1, 3.2:1, 3.1:1, 3:1, 2.9:1, 2.8:1, 2.7:1, 2.6:1, 2.5:1, 2:1, 1.5:1, 1:1.5 and 1: 2.

In one aspect, the concentration of the saussurea involucrate extract is 0.001-0.1g crude drug/ml, such as about 0.001-0.1g crude drug/ml, about 0.002-0.09g crude drug/ml, about 0.003-0.08g crude drug/ml, about 0.004-0.07g crude drug/ml, about 0.005-0.06g crude drug/ml, about 0.006-0.05g crude drug/ml, about 0.007-0.04g crude drug/ml, about 0.008-0.03g crude drug/ml, about 0.009-0.02g crude drug/ml, or about 0.01g crude drug/ml.

In one aspect, the concentration of the bird's nest extract is about 0.001-0.1g crude drug/ml, such as about 0.001-0.1g crude drug/ml, about 0.002-0.09g crude drug/ml, about 0.003-0.08g crude drug/ml, about 0.004-0.07g crude drug/ml, about 0.005-0.06g crude drug/ml, about 0.006-0.05g crude drug/ml, about 0.007-0.04g crude drug/ml, about 0.008-0.03g crude drug/ml, about 0.009-0.02g crude drug/ml, or about 0.01g crude drug/ml.

In one aspect, the aqueous extract of saussurea involucrate can be prepared by the steps of: providing saussurea involucrate; performing ultrasonic extraction in water or aqueous medium; and removing the residue to obtain the aqueous extract of saussurea involucrate. In a specific embodiment, the saussurea involucrate is provided in powder form.

The residue may be removed by means customary in the art, for example by filtration or centrifugation.

In one aspect, the aqueous bird's nest extract may be prepared by the steps of: soaking nidus Collocaliae in water or aqueous medium; removing the liquid; stewing the soaked cubilose in water or an aqueous medium; and removing the cooked bird's nest to obtain the bird's nest water extract.

The liquid may be removed by means customary in the art, for example by filtration.

In one aspect, the composition of the present invention does not contain an extract or an active ingredient other than the saussurea involucrate extract and the cubilose extract.

A second object of the present invention is to provide a product for anti-oxidation and/or whitening, which comprises the composition of the present invention.

The third purpose of the invention is to provide the application of the composition in preparing products for resisting oxidation and/or whitening.

A fourth object of the present invention is to provide the use of the composition of the present invention for anti-oxidation and/or whitening.

A fifth object of the present invention is to provide the composition of the present invention for anti-oxidation and/or whitening.

A sixth object of the present invention is to provide a method of anti-oxidation and/or whitening comprising administering the composition of the present invention to a subject.

A seventh object of the present invention is to provide a method for preparing the composition of the present invention, which comprises: a) providing the saussurea involucrate extractive and the cubilose extractive; and b) mixing the saussurea involucrate extract and the cubilose extract in a weight ratio of about 5:1 to 1: 2.

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments, whereby the above objects of the present invention will be more clearly understood by those skilled in the art.

Drawings

FIG. 1 is a schematic diagram showing the process flow of preparing the composition according to example 1 of the present invention, wherein the weight ratio of the saussurea involucrate extract to the cubilose extract is 3: 1.

FIG. 2 is a standard curve of Trolox absorbance at 570nm according to example 2.

Fig. 3 shows the results of the test of the antioxidant ability of the bird's nest extract, the saussurea involucrate extract, and the composition of the bird's nest extract and the saussurea involucrate extract with different ratios according to example 3 of the present invention.

FIG. 4 shows the results of the inhibition of tyrosinase activity by saussurea involucrate extract, which is a composition of bird's nest extract and saussurea involucrate extract with different ratios according to example 4 of the present invention.

Detailed Description

The invention is based on the surprising discovery of the inventor that the combination of the two north and south medicinal materials of the saussurea involucrate and the cubilose generates unexpected synergistic effect in the aspects of antioxidation and anti-aging. The compatibility can not only obviously enhance the antioxidation/anti-aging effect of the saussurea involucrate, but also enhance the whitening effect of the composition containing the compatibility.

Saussurea involucrate extract

The Saussurea involucrate (Kar.et Kir.) Sch-Bip) used in the invention can be used as a whole plant medicine, namely, the whole herb of the Saussurea involucrate is taken.

The saussurea involucrate extract can be water extract or alcohol extract, and preferably water extract. Those skilled in the art can obtain the saussurea involucrate extract of the invention by adopting the ordinary technical means in the field. For example, as for the aqueous extract of saussurea involucrate, there is no particular limitation on the extraction method that can be used as long as it can sufficiently extract the water-soluble components contained in saussurea involucrate.

The water extract of saussurea involucrate can be obtained by various methods. For example, saussurea involucrate is added to water or aqueous medium, optionally soaked for a period of time, with or without heating. Saussurea involucrate may be provided in powder form to facilitate extraction of water soluble components. Preferably, ultrasonic waves may be additionally applied to shorten the extraction time, or ultrasonic wave extraction may be separately performed without soaking and/or heating. The extraction time can be adjusted according to the amount of water used for soaking herba Saussureae Involueratae, whether heating is applied, whether other auxiliary means (such as ultrasonic wave) is applied, etc. The extraction time may be suitably reduced, for example when heating and/or using ultrasound. Otherwise, the length is correspondingly prolonged.

Removing solid after extraction to obtain herba Saussureae Involueratae extractive solution. The removal of solids can be carried out by means customary in the art, for example, centrifugation, filtration, etc.

The obtained saussurea involucrate extract can be converted into other forms by any means known in the art. For example, by freeze-drying into powder form, to suit different specific uses.

In a specific embodiment, the aqueous extract of saussurea involucrate is prepared by the following steps: providing saussurea involucrate powder; performing ultrasonic extraction in water or aqueous medium; and filtering or centrifuging to remove residue to obtain herba Saussureae Involueratae water extract.

More specifically, herba Saussureae Involueratae is made into powder and sieved, such as No. 3 sieve (mesh size 355 + -13 μm; 50 mesh); ultrasonic extracting with water or aqueous medium 30-50 times, such as 35-45 times, such as 40 times of herba Saussureae Involueratae for 20-40min, preferably 30 min; centrifuging to obtain supernatant. Centrifugation may be carried out at 5000 Xg, for example 5-15min, such as 10 min.

Optionally, the above extraction and centrifugation steps may be repeated, e.g., one or more times, and then the supernatants of the repeated extractions collected together.

After collecting the supernatant, water or aqueous medium can be optionally added to a constant volume of about 0.001-0.1g crude drug/ml, such as about 0.001-0.1g crude drug/ml, about 0.002-0.09g crude drug/ml, about 0.003-0.08g crude drug/ml, about 0.004-0.07g crude drug/ml, about 0.005-0.06g crude drug/ml, about 0.006-0.05g crude drug/ml, about 0.007-0.04g crude drug/ml, about 0.008-0.03g crude drug/ml, about 0.009-0.02g crude drug/ml, or about 0.01g crude drug/ml.

Bird's nest extract

The bird's nest usable in the present invention is not particularly limited, but various types of bird's nests may be used. The bird's nest raw material which can be used in the present invention may be various kinds of bird's nests which are commercially available and naturally harvested, such as but not limited to Indonesia hoax white bird's nests, such as Indonesia white bird's nest and Indonesia white bird's strip, and Thailand white bird's nests, such as Thailand white bird's strip, and the like.

The bird's nest extract of the present invention may be an aqueous extract. One skilled in the art can obtain the cubilose extract which can be used in the invention by adopting the means of ordinary technology in the field. For example, as for the water extract of bird's nest, there is no particular limitation as long as it can sufficiently extract the water-soluble components contained in bird's nest, such as hydrothermal extraction.

In one embodiment, the hydrothermal process comprises the steps of: soaking nidus Collocaliae in water or aqueous medium; filtering to obtain nidus Collocaliae residue; stewing the obtained filter residue in water or water medium; and filtering to remove the filter residue of the stewed bird's nest to obtain the bird's nest extract.

In a further aspect, the hydrothermal treatment comprises adding water or an aqueous medium to the cubilose feedstock and soaking for a period of time sufficient to expand the cubilose feedstock, for example at least 1 hour, for example at least 2 or 3 hours, then filtering off the water or aqueous medium and poaching the expanded cubilose at a temperature of 85 degrees celsius or above for a period of time, for example at least 3 hours, at least 4 hours, at least 5 hours, at least 6 hours and up to 10 hours. In an exemplary embodiment, each kilogram of bird's nest raw material is soaked in about 80-200 liters of water or aqueous medium for swelling for at least 3 hours, after which the water or aqueous medium is removed by filtration, and about 20-40 liters of water or aqueous medium per kilogram of bird's nest raw material is added, then heated to about 85 degrees celsius or above, cooked for at least about 6 hours, after which the cooked bird's nest is removed by filtration.

Removing nidus Collocaliae after stewing, optionally adding water or aqueous medium to a constant volume of about 0.001-0.1g crude drug/ml, such as about 0.001-0.1g crude drug/ml, about 0.002-0.09g crude drug/ml, about 0.003-0.08g crude drug/ml, about 0.004-0.07g crude drug/ml, about 0.005-0.06g crude drug/ml, about 0.006-0.05g crude drug/ml, about 0.007-0.04g crude drug/ml, about 0.008-0.03g crude drug/ml, about 0.009-0.02g crude drug/ml, or about 0.01g crude drug/ml.

The bird's nest raw material can be pretreated by adopting a pretreatment method which is conventional in the field. For hairpins, the surface dirt and the surface attached feathers can be removed by repeatedly rinsing. For the treated cubilose, water can be directly added for soaking and expansion.

In a specific embodiment, the water extract of bird's nest is prepared by the following steps:

a) soaking nidus Collocaliae in water or aqueous medium at about 50-150 times, such as about 60, 70, 80, 90, 100, 110, 120, 130 or 140 times, preferably 100 times, weight of nidus Collocaliae for about 1-5 hours, preferably about 3 hours, e.g. at room temperature, and filtering to obtain nidus Collocaliae residue; and

b) decocting the residue with water or aqueous medium about 20-40 times, such as about 25-35 times, preferably 30 times, the weight of nidus Collocaliae for about 4-8 hr, such as about 5-7 hr, preferably about 6 hr, and filtering to obtain nidus Collocaliae extractive solution, i.e. water extract.

The obtained cubilose extract can be converted into other forms by any means known in the art. For example, by freeze-drying into powder form, to suit different specific uses.

Figure 1 illustrates an exemplary process flow for preparing the composition of the present invention.

Composition comprising a metal oxide and a metal oxide

The composition of the invention is based on the combination of saussurea involucrate extract and cubilose extract.

The saussurea involucrate extract can be extracted by water or alcohol, and the cubilose extract can be water extract in the invention.

In one aspect, the weight ratio of saussurea involucrate extract to cubilose extract contained in the present composition is in the range of about 5:1 to 1:2, preferably about 5:1 to 1:1, e.g. about 5:1, 4.5:1, 4:1, 3.5:1, 3.4:1, 3.3:1, 3.2:1, 3.1:1, 3:1, 2.9:1, 2.8:1, 2.7:1, 2.6:1, 2.5:1, 2:1, 1.5:1, 1:1.5 and 1:2, e.g. 3: 1.

In a specific embodiment, the saussurea involucrate extract is aqueous extract of saussurea involucrate with concentration of about 0.01g crude drug/ml, the bird's nest extract is aqueous extract of bird's nest with concentration of about 0.01g crude drug/ml, and the weight ratio of the saussurea involucrate extract to the bird's nest extract is about 3: 1.

In a particular embodiment, the composition comprises an aqueous extract of saussurea involucrate and an aqueous extract of cubilose in a weight ratio of about 3.5:1 to 2.5:1 (e.g., 3.4:1, 3.3:1, 3.2:1, 3.1:1, 3:1, 2.9:1, 2.8:1, 2.7:1, 2.6:1), and the concentration of herba Saussureae Involueratae extract is about 0.006g crude drug/ml to 0.015g crude drug/ml (such as about 0.007-0.014g crude drug/ml, 0.008-0.013g crude drug/ml, 0.009-0.012g crude drug/ml, 0.010-0.011g crude drug/ml), the nidus Collocaliae water extract has a concentration of about 0.006g crude drug/ml to 0.015g crude drug/ml (e.g. about 0.007-0.014g crude drug/ml, 0.008-0.013g crude drug/ml, 0.009-0.012g crude drug/ml, 0.010-0.011g crude drug/ml).

Those skilled in the art will appreciate that the volume ratio of the two can be adjusted moderately with the concentration of the extract, and that the composition so adjusted can be expected to achieve synergistic effects in terms of antioxidant/anti-aging and improved whitening effects relative to the saussurea involucrate extract alone.

For example, when the volume of the prepared herba saussureae involucratae water extract is about 0.01g crude drug/ml, and the volume of the prepared nidus Collocaliae water extract is 0.01g crude drug/ml, the two are combined together by mixing, for example, in a weight/volume ratio of 3: 1. In accordance with the weight ratio, when the prepared herba saussureae involucratae water extractive solution has a constant volume of about 0.03g crude drug/ml and the prepared nidus Collocaliae water extractive solution has a constant volume of 0.01g crude drug/ml, the two are mixed together at a volume ratio of 1:1, for example.

As used herein, the unit "g crude drug/ml" refers to the number of g of raw materials contained per ml of extract.

As used herein, the term "about" when used to define a numerical value means a range of ± 5% of the indicated numerical value, e.g., ± 4%, ± 3%, ± 2% or ± 1%.

Those skilled in the art will appreciate that details not described in detail herein relating to the compositions of the present invention and products and processes of manufacture thereof may be resorted to by those skilled in the art. For example, when referring to mixing the bird's nest extract with the saussurea involucrate extract, mixing means commonly used in the art, such as mechanical mixing, stirring mixing, automatic mixing suitable for an automated process, and the like, may be employed. For example, the water used for preparing the water extract may be tap water, spring water, mineral water, distilled water, etc., which are common in the art. For another example, the saussurea involucrate powder can be prepared by ordinary techniques in the field, such as grinding.

The composition according to the present invention may contain only the saussurea involucrate extract and the bird's nest extract in the weight ratio within the scope of the present invention, and may further contain other beneficial components according to other desired effects, and may further contain auxiliary components such as acceptable carriers according to the use of the composition (e.g., for cosmetics, foods, health products, etc.) to achieve other effects in addition to oxidation resistance and whitening.

For example, the compositions of the present invention may also be used to formulate medicaments with pharmaceutical carriers and/or other pharmaceutically active ingredients, depending on the actual requirements. The compositions of the invention may also be formulated into cosmetic products with cosmetic carriers and/or other active ingredients; can be used as food additive for health products, foods, beverages, etc.; can be used as nutrition enhancer for nutritional products.

It will also be appreciated by those skilled in the art that the compositions of the present invention can be used directly in different applications, or as a feedstock material for different products, different applications, which are further processed into different products and used widely.

The composition or product according to the invention may be presented in various forms, such as a pharmaceutical, nutraceutical, food, beverage, food additive, dietary supplement or cosmetic product.

The compositions or products of the invention may be consumed directly or otherwise administered to a subject in need thereof, e.g., a human, and may, for example, be provided to the subject in vivo, e.g., orally, or in vitro, e.g., topically to the skin.

Saussurea involucrate and bird's nest are traditional rare and precious Chinese medicinal materials. In a high-cold area with 3,000-4,000 meters of the original Tianshan mountain of the Tianshan snow lotus, because a large amount of manual mining is carried out, wild resources are gradually exhausted, and the yield of the Tianshan snow lotus with high quality is not large due to the difficulty of artificial culture. The invention can reduce the dosage of the saussurea involucrate and generate unexpected synergistic effect on the aspects of oxidation resistance and aging resistance by combining the saussurea involucrate and the cubilose. The compatibility can not only obviously enhance the antioxidation/anti-aging effect of the saussurea involucrate, but also enhance the whitening effect of the composition containing the compatibility, and can be widely applied to various related products such as medicines, health products, foods and beauty products. The preparation method of the composition has simple steps and easy operation, greatly simplifies the preparation process and is beneficial to industrial production.

The saussurea involucrate and the edible bird's nest are the breakthrough of the combination of the north and south medicines except the synergic complementation of the efficacies. The inventor combines the saussurea involucrate produced from the top of Tianshan mountain in the arctic-arctic cold region with the cubilose in the cave on java island in Indonesia, and the breakthrough combination becomes an example of the development of new traditional Chinese medicine resources later.

Examples

The present invention will be described in detail with reference to specific examples. However, those skilled in the art will appreciate that the specific embodiments are merely illustrative of the invention more clearly and are not to be considered in any way as limiting the invention. Unless otherwise indicated, all reagents and equipment are commercially available and all amounts are by weight.

Example 1: preparation of the extract

Taking 10g of Tianshan saussurea involucrate of the natural Tianshan saussurea involucrate farm in Xinjiang, grinding into powder, and sieving with a No. 3 sieve. Ultrasonic extracting with water (400mL) 40 times the weight of herba Saussureae Involueratae for 30 min; centrifuging at 5000 Xg for 10min, and collecting supernatant; and repeating the steps. Collecting the supernatant, adding water to a constant volume of 0.010g crude drug/ml (namely constant volume of 1L), and obtaining the saussurea involucrate extract.

Soaking 10g of Indonesia bigelovii nest in 100 times of water (1L) at room temperature for 3 hr, and filtering to obtain filter residue; stewing and boiling filter residues of the cubilose for 6 hours by using water (300mL) with the weight 30 times that of the cubilose; filtering to remove residue, adding water to constant volume of 0.010g crude drug/ml (constant volume of 1L), and collecting nidus Collocaliae extractive solution.

Example 2: test for antioxidant Effect

A standard curve is established based on the copper ion oxidation resistance of TROLOX (an analogue of vitamin E), and the total oxidation resistance of different nidus Collocaliae extracts and herba Saussureae Involueratae extracts is calculated according to the standard curve. In this test, the higher the TROLOX equivalent, the better the antioxidant capacity of the tested extract (see FIG. 2).

Example 3: synergistic effect of saussurea involucrate extract and cubilose extract

This example shows the synergistic effect of saussurea involucrate extract and cubilose extract in antioxidant capacity.

The saussurea involucrate extract, the bird's nest extract and the composition prepared according to example 1 in the weight ratio of 1:1, 3:1 and 5:1 were respectively weighed. Antioxidant capacity tests were performed in the following experimental groups to compare the effect of the compositions relative to the individual components.

Control group: water without any test substance as blank

Group 1: 1:1 group

a: composition of 12 weight parts (1 g) saussurea involucrate extract and 12 weight parts (1 g) cubilose extract

b: 12 parts by weight (i.e. 1 g) of saussurea involucrate extract and 12 parts by weight (i.e. 1 ml) of water

c: 12 parts by weight (i.e. 1 g) of cubilose extract supplemented with 12 parts by weight (i.e. 1 ml) of water

Group 2: 3:1 group

a: composition of 18 weight parts (1.5 g) saussurea involucrate extract and 6 weight parts (0.5 g) cubilose extract

b: 18 parts by weight (i.e., 1.5 g) of saussurea involucrate extract and 6 parts by weight (i.e., 0.5 ml) of water

c: 6 parts by weight (i.e. 0.5 g) of cubilose extract supplemented with 18 parts by weight (i.e. 1.5 ml) of water

Group 3: 5:1 group

a: composition of 20 weight parts (1.67 g) saussurea involucrate extract and 4 weight parts (0.33 g) cubilose extract

b: 20 parts by weight (i.e., 1.67 g) of saussurea involucrate extract and 4 parts by weight (i.e., 0.33 ml) of water

c: 4 parts by weight (i.e. 0.33 g) of cubilose extract supplemented with 20 parts by weight (i.e. 1.67 ml) of water

Three replicates were set up for each set of experiments and the average of three replicates was taken as the test result.

The test results are shown in fig. 3. Therefore, the tested combination of the cubilose extract and the saussurea involucrate extract with different proportions shows synergistic antioxidant capacity.

Alternative experiments were performed with other sources of bird's nests and found that the synergy was not limited to the specific source of bird's nests (data not shown). Without being bound to a particular theory, the inventors speculate that this is related to the consistency of the bird's nest in its antioxidant efficacy, and thus the consistency of the active ingredients that exert antioxidant efficacy.

Example 4: whitening effect test of composition of saussurea involucrate extract and cubilose extract

The test for tyrosinase activity inhibition is a conventional test method for testing whitening effects. Wherein tyrosine is converted into levodopa (L-DOPA) and Dopachrome (Dopachrome) by tyrosinase. The DOPAchrome has absorbance at 492nm, so that the activity of the tyrosinase can be quantified and calculated. Dopachrome is only a temporary storage and it is converted to Melanin (Melanin). As mentioned above, melanin on human skin is transformed from dopachrome produced by Melanocytes (Melanocytes).

This example employs a tyrosinase activity inhibition assay as is conventional in the art, wherein 0.5mM levodopa is placed in a 50mM sodium phosphate solution at pH 6.8, the tyrosinase concentration is 0.2mg/mL, and the control or sample volume for the assay is 200. mu.L. As a blank control, water without any added extract was used, and 24 parts by weight of each of the compositions of group 1a, group 2a and group 3a in example 3 and 24 parts by weight of the saussurea involucrate extract prepared according to example 1 alone (i.e., 2g of saussurea involucrate extract) were used as different test samples.

And placing the control and different test samples in a 96-well plate, standing for 20 minutes at room temperature under the condition of no light, reading the absorbance at the wavelength of 492nm by using a plate reader, and calculating the inhibition rate of the different test samples on tyrosinase.

Three replicates were set for each set of tests and the average of three replicates was taken as the test result. The test results are shown in fig. 4. Therefore, compared with the single saussurea involucrate extract, the whitening effect of the composition with different proportions is obviously improved. This shows that the composition of the present invention can exert a sufficient whitening effect while having a synergistically beneficial antioxidant effect.

As proved by the application, the bird's nest is matched with the saussurea involucrate to be used, and the integral oxidation resistance and the whitening effect of the formula are fully exerted in a synergistic and beneficial mode. The preparation process is simple, the cost can be reduced, and increasingly deficient natural precious resources of the wild saussurea involucrate can be protected. Meanwhile, under the condition of the current shortage of traditional Chinese medicine resources, the invention is an innovative solution with foresight by combining and applying the very rare compatibility of north-south cross-border of the saussurea involucrate and the cubilose.

As previously mentioned, the present invention has been specifically explained in detail in connection with one or more embodiments. However, it is not to be understood that the scope of the present invention is limited thereby. It should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the spirit and substance of the present invention, and these changes and modifications are within the scope of the present invention.

Claims

1. A product for resisting oxidation and/or whitening, which consists of saussurea involucrate extract and cubilose extract, and is characterized in that:

wherein the weight ratio of the saussurea involucrate extract to the cubilose extract is 3: 1;

wherein the saussurea involucrate extract is a saussurea involucrate water extract, and the cubilose extract is a cubilose water extract;

wherein the water extract of saussurea involucrate is prepared by the following steps:

a. providing saussurea involucrate;

b. performing ultrasonic extraction in water or aqueous medium; and

c. removing residue to obtain herba Saussureae Involueratae water extract;

wherein the water extract of cubilose is prepared by the following steps:

a. soaking nidus Collocaliae in water or aqueous medium;

b. removing the liquid;

c. stewing the soaked cubilose in water or an aqueous medium; and

d. removing the stewed bird's nest to obtain the water extract of bird's nest.

2. A method of making the composition of claim 1, comprising:

a) providing saussurea involucrate extract and cubilose extract; and

b) mixing the saussurea involucrate extract and the cubilose extract in a weight ratio of 3: 1.