CN108042433A - A kind of whitening antioxidation plant composition, plant composition zymotic fluid and preparation method and purposes - Google Patents
A kind of whitening antioxidation plant composition, plant composition zymotic fluid and preparation method and purposes Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The present invention provides a kind of plant composition of whitening antioxidation, the plant composition includes by mass percentage：Rhizoma Chuanxiong 15%~50%, the radix paeoniae rubrathe 10%~40%, radix ampelopsis 10%~35%, mulberry leaf 5%~25%；Plant composition zymotic fluid the present invention also provides a kind of whitening antioxidation and its preparation method and application；The plant composition of whitening antioxidation provided by the invention, based on TCM theory, it is reasonably compounded according to the compound principle of monarch, enable the composition to synergy, reach whitening, oxidation resistant effect, meanwhile avoid the whitening agent of traditional chemical synthesis, the irritation that antioxidant is brought.
Description
Technical field
The invention belongs to field of daily chemicals, are related to a kind of plant composition of whitening antioxidation, plant composition hair
Zymotic fluid and its preparation method and application.
Background technology
Peach and cream, moist skin are always what women was pursued, whitening, it is anti-oxidant also be always cosmetics city
The theme of field.Whitening class cosmetics are to add ursin, quinhydrones, kojic acid, BHT, vitamin C and its derivative currently on the market
Based on the chemical synthesis substance with whitening, anti-oxidation efficacy.Although these chemical synthesis effect compound effects are notable,
The problems such as irritation is big, unstable, effect is single is gradually exposed in use.So exploitation security level is high, more work(
Effect, stability are good, cosmetic material from natural plant active ingredient, become cosmetics research and development institution and production in recent years
The hot spot of enterprises pay attention.
Applicating history of the Chinese herbal medicine in China is long, and in ancient Chinese, already there are many for extractions such as plant, Chinese herbal medicines
And the discussion of fermentation method.《Health side》、《Miscellaneous treatment side》、《Compendium of Materia Medica》There is the record of plant fermentation in.Using plant
The advantages of fermentation, is that fermentation temperature is relatively low, fermentation pH is mild, and active components of plants structure is not destroyed, can be more complete
The effect of retaining plant ingredient and its activity.
The species of Chinese herbal medicine is various, has effects that certain curative effect mostly, and side effect is smaller.At present, research reports
Road carries out plant material exploitation using fermentation technique, and improves plant effect.Such as the red ginseng to ferment and common red ginseng phase
Improved than, polyphenol and flavones content and inoxidizability, the metabolite content of ginsenoside increases, therefore crease-resistant effect and
White-skinned face function is improved.
Therefore, how to avoid destroying the structure of active components of plants using traditional high temperature extraction, reasonable development is new
The formula of Chinese herbal medicine avoids the irritation of chemical synthesis preparation, further improves the whitening of natural plant component, oxidation resistant work(
Effect, has important meaning for the development of cosmetics.
The content of the invention
It is an object of the invention to a kind of plant composition of whitening antioxidation and its preparation methods of zymotic fluid and purposes.
For this purpose, the present invention uses following technical scheme：
In a first aspect, the present invention provides a kind of plant composition of whitening antioxidation, the plant composition presses quality
Percentages include：
The plant composition of whitening antioxidation provided by the invention, based on TCM theory, according to answering for monarch
Square principle is reasonably compounded, and enables the composition to synergy, reaches whitening, oxidation resistant effect, meanwhile, it avoids
The irritation that whitening agent, the antioxidant of traditional chemical synthesis are brought.
Above-mentioned 4 kinds of components, can be synergistic according to scientific matching, compared to the activity of one-component, whitening antioxidation
It significantly improves, and lacks any one component, effect can be declined；Its tyrosinase inhibition rate can reach
94.2%, and the inhibiting rate of one-component only has 61%~84%；And DPPH free radical scavenging activities, the clearance rate of plant composition
83.9% is can reach, and the clearance rate of one-component only has 65%~77%.
In the present invention, the mass percent of Rhizoma Chuanxiong is 15%~50% in the plant composition of whitening antioxidation, such as
Can be 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%.
In the present invention, Rhizoma Chuanxiong is monarch drug in a prescription, has promoting circulation of blood, promoting blood circulation, promotes blood circulation of human body, increases human body nutrition and passes
It send, extends the lag time of tyrosinase reaction, reach whitening effect.
In the present invention, the mass percent of the radix paeoniae rubrathe is 10%~40% in the plant composition of whitening antioxidation, such as
Can be 10%, 15%, 20%, 25%, 30%, 35% or 40%.
In the present invention, the radix paeoniae rubrathe is minister, has promoting blood circulation and removing blood stasis, improvement human body microcirculation, anti-oxidant, the system of defense etc. of promotion
Multiple functions, collaboration Rhizoma Chuanxiong have better whitening, anti-ageing effect.
In the present invention, the mass percent of radix ampelopsis is 10%~35% in the plant composition of whitening antioxidation, such as
Can be 10%, 15%, 20%, 25%, 30% or 35%.
Radix ampelopsis is usually used in clearing heat and detoxicating, carbuncle that disappears dissipating bind, and in the present invention, and radix ampelopsis is assistant, have whitening, heat-clearing wind-dispelling,
The effect of myogenic sore.
In the present invention, the mass percent of mulberry leaf is 5%~25% in the plant composition of whitening antioxidation, such as can
To be 5%, 10%, 15%, 20% or 25%.
In the present invention, mulberry leaf have whitening, anti-ageing physiological activator to make rich in mulberry leaf polyphenol, flavones in mulberry leaves etc.,
Above-mentioned 3 kinds of active components can further be assisted to play effect.
Preferably, the plant composition includes by mass percentage：Rhizoma Chuanxiong 30%~45%, the radix paeoniae rubrathe 20%~
35%th, radix ampelopsis 15%~30% and mulberry leaf 5%~15%.
In the present invention, it is preferred to be the component of above-mentioned mass percent, can further improve plant composition whitening,
Antioxygenic property.
Preferably, the plant composition includes by mass percentage：Rhizoma Chuanxiong 40%, the radix paeoniae rubrathe 30%, 20% and of radix ampelopsis
Mulberry leaf 10%.
In the present invention, it is preferred to mass percent is Rhizoma Chuanxiong 40%, the radix paeoniae rubrathe 30%, radix ampelopsis 20% and mulberry leaf 10%, it can be with
Plant composition whitening, antioxygenic property is made to reach optimal, it is clear that tyrosinase inhibition rate is up to 94.2%, DPPH free radicals
Except rate is up to 83.9%.
Second aspect, the present invention provides a kind of plant composition zymotic fluid of whitening antioxidation, the plant compositions
Zymotic fluid includes plant composition, Bacillus acidi lactici bacterium solution and the sterile water of whitening antioxidation as described in relation to the first aspect.
The present invention prepares the plant composition zymotic fluid of whitening antioxidation by the way of microbial fermentation, avoids tradition
High temperature extraction damages the structure of active components of plants, and reaction condition is mild, need not add chemical reagent, product safety
Property it is high, containing substantial amounts of fermenting enzyme in zymotic fluid, the active ingredient in plant material can be extracted sufficiently, can be more complete
Reservation plant the effect of ingredient and its activity.
Preferably, the Bacillus acidi lactici is Lactobacillus gasseri.
Preferably, the bacterial concentration of the Bacillus acidi lactici is 106Cfu/mL~108Cfu/mL, such as can be 106cfu/
mL、107Cfu/mL or 108cfu/mL。
Preferably, the plant composition, the proportioning of sterile water and Bacillus acidi lactici bacterium solution in zymotic fluid are (5~15):
(200~250):(15~25), such as can be 5:200:15、8:210:18、13:200:20 or 15:250:25.
Wherein, the proportioning of plant composition and sterile water is calculated according to mass ratio, and the proportioning of Bacillus acidi lactici bacterium solution is according to body
Product calculates.
Preferably, the plant composition, the proportioning of sterile water and Bacillus acidi lactici bacterium solution in zymotic fluid are 13:200:
20。
In the present invention, it is preferred to plant composition, the proportioning of sterile water and Bacillus acidi lactici bacterium solution in zymotic fluid are 13:
200:20, whitening and anti-oxidation efficacy can be made to reach optimal, and the effect of other proportionings decreases.
The third aspect, the present invention provides a kind of plant composition zymotic fluids of the whitening antioxidation as described in second aspect
Preparation method, the described method comprises the following steps：After plant composition is crushed, sieving, with sterile water, Bacillus acidi lactici bacterium solution
Mixing is fermented, and obtains the plant composition zymotic fluid.
The present invention prepares plant composition zymotic fluid by the method for fermentation, and reaction condition is mild, without chemistry addition examination
Agent, the Product Safety that is prepared is high, the effect of can keeping natural plant ingredient to greatest extent.
Preferably, the mesh number of the sieving is the mesh of 40 mesh~100, such as can be 40 mesh, 50 mesh, 60 mesh, 70 mesh, 80
Mesh, 90 mesh or 100 mesh.
Preferably, the fermentation carries out in shaking table.
Preferably, the temperature of the fermentation is 35 DEG C~40 DEG C, such as can be 35 DEG C, 36 DEG C, 37 DEG C, 38 DEG C, 39 DEG C
Or 40 DEG C.
Preferably, the rotating speed of the fermentation be 80r/min~150r/min, such as can be 80r/min, 90r/min,
100r/min, 110r/min, 120r/min, 130r/min, 140r/min or 150r/min.
Preferably, the time of the fermentation be 40h~60h, such as can be 40h, 42h, 45h, 48h, 50h, 52h,
53h, 55h, 56h, 58h or 60h.
In the present invention, further included after fermentation tunning is centrifuged, is collected supernatant, cross film obtain the plant
Composition zymotic fluid.
Preferably, the rotating speed of the centrifugation be 10000r/min~12000r/min, such as can be 10000r/min,
11000r/min, 11500r/min, 11600r/min, 11800r/min or 12000r/min.
Preferably, the time of the centrifugation be 20min~40min, such as can be 20min, 23min, 25min,
26min, 28min, 30min, 35min or 40min.
Preferably, the filter membrane for crossing film is 0.22 μm of filter membrane.
The preparation method of the plant composition zymotic fluid of whitening antioxidation provided by the invention, comprises the following steps：
(1) after crushing plant composition, crossing the mesh sieve of 40 mesh~100, mix with sterile water, Bacillus acidi lactici bacterium solution, proportioning
For (5~15) g:(200~250) g:(15~25) mL, in 35 DEG C~40 DEG C, rotating speed is 80r/min~150r/min shaking tables
It ferments, obtains tunning；
(2) by tunning in rotating speed to centrifuge 20min~40min under 10000r/min~12000r/min, in collection
Clear liquid crosses 0.22 μm of filter membrane, collects filtrate and obtains the plant composition zymotic fluid.
Fourth aspect, the present invention provides a kind of plant composition zymotic fluids of the whitening antioxidation as described in second aspect
Purposes in facial mask and facial treatment milk is prepared.
When plant composition zymotic fluid provided by the invention is applied in facial mask and facial treatment milk, compared to un-added production
Product improve whitening, oxidation resistant effect.
Compared with the prior art, the invention has the advantages that：
(1) plant composition of whitening antioxidation provided by the invention, based on TCM theory, according to monarch
Compound principle is reasonably compounded, and enables the composition to synergy, reaches whitening, oxidation resistant effect, meanwhile, it avoids
The irritation that whitening agent, the antioxidant of traditional chemical synthesis are brought.4 kinds of components, can be synergistic according to scientific matching, phase
Than in the activity of one-component, whitening antioxidation significantly improves, and lacks any one component, effect can under
Drop；Its tyrosinase inhibition rate can reach 94.2%, and the inhibiting rate of one-component only has 61%~84%；And DPPH is certainly
By base clearance rate, the clearance rate of plant composition can reach 83.9%, and the clearance rate of one-component only have 65%~
77%.
(2) present invention prepares the plant composition zymotic fluid of whitening antioxidation by the way of microbial fermentation, avoids
Conventional high-temperature extraction damages the structure of active components of plants, and reaction condition is mild, need not add chemical reagent, product
Safe, containing substantial amounts of fermenting enzyme in zymotic fluid, the active ingredient in plant material can be extracted sufficiently, can be compared with
Complete the effect of retaining plant ingredient and its activity.
Specific embodiment
The technical solution further illustrated the present invention below by specific embodiment.Those skilled in the art should be bright
, the embodiment be only to aid in understand the present invention, be not construed as to the present invention concrete restriction.
In the present invention, Lactobacillus gasseri is purchased from Guangdong Microbes Inst Culture Collection.It is being sent out
Before ferment, strain is enlarged culture first, incubation step is as follows：
Actication of culture：Picking colony is put into MRS fluid nutrient mediums from the inclined-plane of preservation, 37 DEG C of static gas wave refrigerator 36h-
48h。
Purifying agaric：By the strain gradient dilution bed board of activation, to obtain single bacterium colony.
Strain spreads cultivation：Picking single bacterium colony is incubated at MRS fluid nutrient mediums, and after 12h, MRS liquid is inoculated in by 2% inoculum concentration
Culture medium, 37 DEG C of static gas wave refrigerators for 24 hours, adjust bacterial concentration to 106cfu/mL-108cfu/mL。
Embodiment 1
Plant composition zymotic fluid is prepared by following steps in the present embodiment
(1) Rhizoma Chuanxiong 5.2g, radix paeoniae rubrathe 3.9g, radix ampelopsis 2.6g and mulberry leaf 1.3g are weighed in proportion, are crushed, it, will after crossing 50 mesh sieves
106-108The bacterium solution of cfu/mL Lactobacillus gasseris is seeded in plant composition and sterile water, plant composition, water and bacterium solution
It matches as 13g:200g:20mL carries out fermentation 48h in 37 DEG C, rotating speed is 100r/min shaking tables, obtains tunning；
(2) tunning is centrifuged into 30min in the case where rotating speed is 12000r/min, wherein centrifugation radius is 9cm, collects supernatant
Liquid, crosses 0.22 μm of filter membrane, and filtration sterilization collects filtrate and obtains plant composition zymotic fluid.
Embodiment 2
Plant composition zymotic fluid is prepared by following steps in the present embodiment
(1) Rhizoma Chuanxiong 4.55g, radix paeoniae rubrathe 3.25g, radix ampelopsis 2.6g and mulberry leaf 2.6g are weighed in proportion, are crushed, after crossing 50 mesh sieves,
By 106-108The bacterium solution of cfu/mL Lactobacillus gasseris is seeded in plant composition and sterile water, plant composition, water and bacterium solution
Proportioning be 13g:200g:20mL carries out fermentation 48h in 37 DEG C, rotating speed is 100r/min shaking tables, obtains tunning；
(2) tunning is centrifuged into 30min in the case where rotating speed is 12000r/min, wherein centrifugation radius is 9cm, collects supernatant
Liquid, crosses 0.22 μm of filter membrane, and filtration sterilization collects filtrate and obtains plant composition zymotic fluid.
Embodiment 3
Plant composition zymotic fluid is prepared by following steps in the present embodiment
(1) Rhizoma Chuanxiong 3.9g, radix paeoniae rubrathe 3.25g, radix ampelopsis 3.25g and mulberry leaf 2.6g are weighed in proportion, are crushed, after crossing 50 mesh sieves,
By 106-108The bacterium solution of cfu/mL Lactobacillus gasseris is seeded in plant composition and sterile water, plant composition, water and bacterium solution
Proportioning be 13g:200g:20mL carries out fermentation 48h in 37 DEG C, rotating speed is 100r/min shaking tables, obtains tunning；
(2) tunning is centrifuged into 30min in the case where rotating speed is 12000r/min, wherein centrifugation radius is 9
Cm is collected supernatant, and crosses 0.22 μm of filter membrane, and filtration sterilization collects filtrate and obtains plant composition zymotic fluid.
Embodiment 4
Plant composition zymotic fluid is prepared by following steps in the present embodiment
(1) Rhizoma Chuanxiong 2g, radix paeoniae rubrathe 1.9g, radix ampelopsis 3.1g and mulberry leaf 2g are weighed in proportion, is crushed, after crossing 40 mesh sieves, by 106-
108The bacterium solution of cfu/mL Lactobacillus gasseris is seeded in plant composition and sterile water, the proportioning of plant composition, water and bacterium solution
For 15g:250g:25mL carries out fermentation 40h in 35 DEG C, rotating speed is 80r/min shaking tables, obtains tunning；
(2) tunning is centrifuged into 20min in the case where rotating speed is 10000r/min, wherein centrifugation radius is 9
Cm is collected supernatant, and crosses 0.22 μm of filter membrane, and filtration sterilization collects filtrate and obtains plant composition zymotic fluid.
Embodiment 5
Plant composition zymotic fluid is prepared by following steps in the present embodiment
(1) Rhizoma Chuanxiong 5.0g, radix paeoniae rubrathe 1.0g, radix ampelopsis 3.5g and mulberry leaf 0.5g are weighed in proportion, are crushed, it, will after crossing 100 mesh sieves
106-108The bacterium solution of cfu/mL Lactobacillus gasseris is seeded in plant composition and sterile water, plant composition, water and bacterium solution
It matches as 5g:200g:15mL carries out fermentation 60h in 40 DEG C, rotating speed is 150r/min shaking tables, obtains tunning；
(2) tunning is centrifuged into 40min in the case where rotating speed is 12000r/min, wherein centrifugation radius is 9
Cm is collected supernatant, and crosses 0.22 μm of filter membrane, and filtration sterilization collects filtrate and obtains plant composition zymotic fluid.
Embodiment 6
Difference lies in Rhizoma Chuanxiong, the radix paeoniae rubrathe, radix ampelopsis and mulberry leaf are in plant composition in this implementation with embodiment 1 for the present embodiment
In mass percent be respectively 30%, 30%, 25% and 15%, remaining preparation method is same as Example 1, is prepared
Plant composition zymotic fluid.
Embodiment 7
Difference lies in Rhizoma Chuanxiong, the radix paeoniae rubrathe, radix ampelopsis and mulberry leaf are in plant composition in this implementation with embodiment 1 for the present embodiment
In mass percent be respectively 45%, 20%, 20% and 15%, remaining preparation method is same as Example 1, is prepared
Plant composition zymotic fluid.
Comparative example 1
Difference lies in a kind of plant component, Rhizoma Chuanxiong 13g and bacterium is only used only in this comparative example to this comparative example with embodiment 1
Liquid, water are fermented to obtain Rhizoma Chuanxiong zymotic fluid.
Comparative example 2
Difference lies in a kind of plant component, radix paeoniae rubrathe 13g and bacterium is only used only in this comparative example to this comparative example with embodiment 1
Liquid, water are fermented to obtain radix paeoniae rubrathe zymotic fluid.
Comparative example 3
Difference lies in a kind of plant component, radix ampelopsis 13g and bacterium is only used only in this comparative example to this comparative example with embodiment 1
Liquid, water are fermented to obtain radix ampelopsis zymotic fluid.
Comparative example 4
Difference lies in a kind of plant component, mulberry leaf 13g and bacterium is only used only in this comparative example to this comparative example with embodiment 1
Liquid, water are fermented to obtain mulberry leaf zymotic fluid.
Comparative example 5
Difference lies in use Rhizoma Chuanxiong 7.0g, the radix paeoniae rubrathe to this comparative example with embodiment 1 in plant composition in this comparative example
0.5g, radix ampelopsis 0.5g and mulberry leaf 5.2g, then obtain plant composition zymotic fluid with bacterium solution, water by fermentation.
Comparative example 6
Difference lies in plant composition, sterile water and Bacillus acidi lactici bacterium solutions in this comparative example with embodiment 1 for this comparative example
Proportioning be 2g:120g:10mL carries out fermentation and plant composition zymotic fluid is prepared.
Comparative example 7
This comparative example and embodiment 1 difference lies in, plant composition only includes Rhizoma Chuanxiong 8g and radix paeoniae rubrathe 4g in this comparative example,
Then plant composition zymotic fluid is obtained with bacterium solution, water by fermentation.
Comparative example 8
This comparative example and embodiment 1 difference lies in, in this comparative example plant composition only include Rhizoma Chuanxiong 8g, radix ampelopsis 4g and
Radix paeoniae rubrathe 6g then obtains plant composition zymotic fluid with bacterium solution, water by fermentation.
Comparative example 9
Difference lies in plant composition only includes Rhizoma Chuanxiong 8g and radix ampelopsis to this comparative example in this comparative example with embodiment 1
4g then obtains plant composition zymotic fluid with bacterium solution, water by fermentation.
Comparative example 10
This comparative example and embodiment 1 difference lies in, in this comparative example plant composition only include Rhizoma Chuanxiong 8g, radix ampelopsis 4g
With mulberry leaf 2g, then plant composition zymotic fluid is obtained with bacterium solution, water by fermentation.
Zymotic fluid prepared by embodiment 1-7 and comparative example 1-10 carries out in-vitro evaluation test experiments, anti-oxidation efficacy point
Analysis experiment, patch experiment and normal individual trial evaluation experimental, specific test are as follows：
(1) in-vitro evaluation test (tyrosinase inhibits test)
Tyrosinase is the key enzyme of melanin generation, it controls its level of activity of the forming process of melanin to pigment
Deposition play a major role.Current market sales of many whitening products are all to reach whitening function to inhibit tyrosinase,
Therefore be to evaluate the leading indicator of skin-lightening cosmetic to the power of tyrosinase inhibitory action, test method is as follows：
The zymotic fluid prepared using embodiment 1-7 and comparative example 1-10 is sample solution, according to 1 column data sample-adding of table, and
It is placed on constant temperature 10min in 37 DEG C of water-baths；Tyrosinase is added in, solution is placed in 37 DEG C of water-baths after constant temperature 10min, in
Its absorbance is measured under 475nm.
Table 1
| Reaction solution ingredient | Blank control | Blank | Sample controls | Sample |
| Sample solution/mL | - | - | 1.0 | 1.0 |
| PBS (pH=6.8)/mL | 3.0 | 2.0 | 2.0 | 1.0 |
| L-tyrosine/mL | 2.0 | 2.0 | 2.0 | 2.0 |
| Tyrosinase/mL | - | 1.0 | - | 1.0 |
| Total volume/mL | 5.0 | 5.0 | 5.0 | 5.0 |
Calculate inhibitory activity against tyrosinase, wherein inhibitory activity against tyrosinase=[1- (A2/A1)] × 100%, wherein
A1For the absorbance of blank；A2For the absorbance of sample, the result of calculating is as shown in table 2 below：
Table 2
The present invention is can be seen that by embodiment 1 and embodiment 4-5 comparative examples to provide in composition, Rhizoma Chuanxiong 30%~
45%th, the radix paeoniae rubrathe 20%~35%, radix ampelopsis 15%~30% and mulberry leaf 5%~15%；Plant composition, sterile water and Bacillus acidi lactici
Proportioning of the bacterium solution in zymotic fluid is (5~15) g:(200~250) g:During (15~25) mL, the tyrosinase of zymotic fluid inhibits
Rate can reach highest, and effect is best；By embodiment 4-5 with
The comparison of embodiment 6-7 is as can be seen that the scope of further preferred plant component of the invention, effect is compared with excellent
Select the effect outside scope more preferable；By embodiment 1-5 and the comparison of comparative example 1-4,7-10 as can be seen that using only a kind of plant group
Point, effect can decline, and comparative example 3-4 effects are worst, lack any one or two kinds of components, inhibiting rate are also declined；
By example 1-5 and comparative example 5-6 comparisons as can be seen that the mass percent and composition of composition, bacterium solution and water are matched somebody with somebody
Than not in the framework of the present definition when, inhibiting rate can also be declined.
(2) anti-oxidation efficacy analysis experiment
With the antioxidation in vitro performance for the method evaluation sample for removing DPPH free radicals, ethanol solution is in wavelength 517nm
There is absorption maximum at place, in purple.In the presence of having free radical scavenger, with its electron pairing, make UV absorption remitted its fury very
To disappearance, and to it to receive electron amount directly proportional for fading extent.Therefore, sample can be evaluated by measuring the variation of light absorption value
Antioxidation activity.
It is as follows：
Take three PE pipe, the deionized water of an addition 2mL, another two each prepare liquids for adding in 2mL, be respectively labeled as i,
J, k sequentially adds the DPPH solution of 2mL into i and j pipes, adds in 2mL absolute ethyl alcohols in k pipes, be uniformly mixed, be protected from light standing
30min, with ultraviolet specrophotometer at 517nm wavelength, its absorbance of sequentially determining.
Clearance rate calculation formula is：Clearance rate (%)=[1- (Aj-Ak)/Ai] × 100%, in formula：AiIt is molten for DPPH ethyl alcohol
The absorbance of liquid；AjThe absorbance of free radical is removed for prepare liquid；AkFor the absorbance of prepare liquid.
The result being calculated is as shown in table 3 below：
Table 3
It can be concluded that plant composition zymotic fluid is for DPPH free radicals by anti-oxidation efficacy analysis experiment
The trend that the influence of clearance rate is presented with tyrosinase inhibition rate has identical effect, is limited hence it is demonstrated that of the invention
Composition can make whitening, anti-oxidation efficacy reach highest.
(3) plant composition zymotic fluid patch is tested
Using 22-40 Sui men and women volunteer of 30 ages as object (male 11 people, 19 people of female).Substances are respectively to be diluted to
Embodiment 1, comparative example 1-2 and the comparative example 8-9 and deionized water of 10% aqueous solution.
Experiment process：With syringe 0.02mL tested materials is taken to be put into spot examination device and (include special filter paper piece), then will be added with
The spot examination device of tested material spreads on the back of subject with medical adhesive tape, is gently pressed with palm and is allowed to equably be pasted on skin, held
Continue for 24 hours；After for 24 hours, spot of tearing examination device, and indicate sample and blank control position.And in tear spot examination device after 30min, for 24 hours and
48h assesses place of application and records result.
Wherein, the extent of reaction, the dermoreaction corresponding to grading system are as shown in table 4 below：
Table 4
The result of test is as shown in table 5 below：
Table 5
From test result table 5, during using single plant herb fermenting, can all there be suspicious reaction, but it is also only faint
Erythema；And during 2 kinds of component proportions, erythema weakens, and whens 3 kinds of component proportions further weakens；And 4 kinds of tcm components carry out rationally again
There is not suspicious reaction to human body with the obtained zymotic fluid of combination.Thus illustrate that there is whitening, anti-oxidation efficacy in the present invention
Plant composition zymotic fluid has security, does not cause adverse reaction to human body.
Experimental example 1
Plant composition zymotic fluid prepared by embodiment 1 is prepared into facial treatment milk, and is compared with contrast experiment's example 1.
Specific formula such as the following table 6：
Table 6
Facial treatment milk preparation method is as follows：Oil phase in formula table is put into oil phase pot, is heated to 85 DEG C, stirring to dissolving
Uniformly；Water in formula table is mutually put into water phase pot, is heated to 85 DEG C, stirs to being completely dissolved, oil phase is slowly pumped into water
Phase pot, with the rate emulsifying 5~10 minutes of 4000~6000rpm, stirring be cooled to 45 DEG C add in essence, Phenoxyethanol,
Plant composition zymotic fluid simultaneously stirs evenly, and is cooled to the related physical and chemical index of 38 DEG C of detections, discharges after qualified, as with whitening
With anti-oxidation efficacy facial treatment milk.
The plant fermented liquid in the present invention is not added in common facial treatment milk preparation method.
Experimental example 2
Plant composition zymotic fluid prepared by embodiment 1 is prepared into facial mask, and is compared with contrast experiment's example 2.Specifically
Formula such as the following table 7：
Table 7
The preparation method of facial mask is as follows：
Methyl hydroxybenzoate and Nipasol are dissolved by heating in butanediol in advance to transparent, essence is in PEG-60 hydrogenated castors
It is pre-dissolved in oil.Water in formula table is added to water phase pot, is heated to 85 DEG C, to being completely dissolved, stirring is cooled to 45 DEG C for stirring
Addition phase is added in, stirring is to being completely dissolved.The related physical and chemical index of 38 DEG C of detections is cooled to, is discharged after qualified, as with whitening
With the facial mask of anti-oxidation efficacy.
The plant fermented liquid in the present invention is not added in common facial mask preparation method.
Facial mask prepared by experimental example 2 carries out normal individual trial evaluation experimental：
Experimental method：The facial mask prepared in experimental example 2 is chosen as laboratory sample, carries out on probation comment to volunteer respectively
Valency.Choose dark, the deeper volunteer of 90 colours of skin, each 45 people of men and women is randomly divided into 3 groups, every group of 15 males, 15 female
Property.3 groups of volunteers are respectively using 5 whitening antioxidation facial mask of embodiment, 6 common facial mask of comparative example, the sample of commercially available whitening mask.
Application method：It uses, using once, is used continuously 4 weeks after face cleaning within every two days, improving situation according to the colour of skin carries out sensory evaluation.
Standards of grading for 1 point-without effect, 2 points-effective, very weak, 3 points-effective, with obvious effects, 4 points-effective, effect
Fruit is preferable, 5 points-effective, effect it is very good.Scoring " >=3 points " is judged as with obvious effects, and counts scoring " >=3 points "
Total number of persons.Obtain that the results are shown in Table 8：
Table 8
More than volunteer's sensory evaluation data explanation：There is the composite plant of whitening and anti-oxidation efficacy hair containing the present invention
The facial mask of zymotic fluid has effects that clearly for common facial mask, and is better than commercially available whitening mask.
Applicant state, the present invention by above-described embodiment come illustrate the present invention whitening antioxidation plant composition,
Plant composition zymotic fluid and its preparation method and application, but the invention is not limited in above-mentioned method detailed, that is, do not mean that
The present invention, which has to rely on above-mentioned method detailed, to be implemented.Person of ordinary skill in the field is it will be clearly understood that the present invention's
Any improvement, the addition of equivalence replacement and auxiliary element to each raw material of product of the present invention, the selection of concrete mode etc., all falls within
Within protection scope of the present invention and the open scope.
Claims (10)
1. a kind of plant composition of whitening antioxidation, which is characterized in that the plant composition includes by mass percentage：
2. plant composition according to claim 1, which is characterized in that the plant composition wraps by mass percentage
It includes：Rhizoma Chuanxiong 30%~45%, the radix paeoniae rubrathe 20%~35%, radix ampelopsis 15%~30% and mulberry leaf 5%~15%；
Preferably, the plant composition includes by mass percentage：Rhizoma Chuanxiong 40%, the radix paeoniae rubrathe 30%, radix ampelopsis 20% and mulberry leaf
10%.
3. the plant composition zymotic fluid of a kind of whitening antioxidation, which is characterized in that the plant composition zymotic fluid is included such as
Plant composition, Bacillus acidi lactici bacterium solution and the sterile water of the whitening antioxidation of claim 1 or 2.
4. plant composition zymotic fluid according to claim 3, which is characterized in that the Bacillus acidi lactici is Jia Shi breast bars
Bacterium；
Preferably, the bacterial concentration of the Bacillus acidi lactici is 106Cfu/mL~108cfu/mL。
5. the plant composition zymotic fluid according to claim 3 or 4, which is characterized in that the plant composition, sterile water
It is (5~15) with proportioning of the Bacillus acidi lactici bacterium solution in zymotic fluid:(200~250):(15~25)；
Preferably, the plant composition, the proportioning of sterile water and Bacillus acidi lactici bacterium solution in zymotic fluid are 13:200:20.
6. the preparation method of the plant composition zymotic fluid of the whitening antioxidation according to any one of claim 3-5,
It is characterized in that, the described method comprises the following steps：After plant composition is crushed, sieving, mixed with Bacillus acidi lactici bacterium solution, sterile water
Conjunction is fermented, and obtains the plant composition zymotic fluid.
7. according to the method described in claim 6, it is characterized in that, the mesh number of the sieving is the mesh of 40 mesh~100；
Preferably, the fermentation carries out in shaking table；
Preferably, the temperature of the fermentation is 35 DEG C~40 DEG C；
Preferably, the rotating speed of the fermentation is 80r/min~150r/min；
Preferably, the time of the fermentation is 40h~60h.
8. the method according to claim 6 or 7, which is characterized in that further included after the fermentation by tunning carry out from
The heart, collect supernatant, cross film obtain the plant composition zymotic fluid；
Preferably, the rotating speed of the centrifugation is 10000r/min~12000r/min；
Preferably, the time of the centrifugation is 20min~40min；
Preferably, the filter membrane for crossing film is 0.22 μm of filter membrane.
9. according to the method any one of claim 6-8, which is characterized in that the described method comprises the following steps：
(1) after crushing plant composition, crossing the mesh sieve of 40 mesh~100, mix, matched as (5 with sterile water, Bacillus acidi lactici bacterium solution
~15) g:(200~250) g:(15~25) mL is carried out in 35 DEG C~40 DEG C, rotating speed is 80r/min~150r/min shaking tables
Fermentation, obtains tunning；
(2) tunning is centrifuged into 20min~40min in the case where rotating speed is 10000r/min~12000r/min, collected supernatant,
0.22 μm of filter membrane is crossed, filtrate is collected and obtains the plant composition zymotic fluid.
10. the plant composition zymotic fluid of the whitening antioxidation according to any one of claim 3-9 prepare facial mask and
Purposes in facial treatment milk.
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