CN108026591B - 诊断方法和组合物 - Google Patents

诊断方法和组合物 Download PDF

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Publication number
CN108026591B
CN108026591B CN201580062485.XA CN201580062485A CN108026591B CN 108026591 B CN108026591 B CN 108026591B CN 201580062485 A CN201580062485 A CN 201580062485A CN 108026591 B CN108026591 B CN 108026591B
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primer
amplification reaction
nucleic acid
region
genetic element
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Chinese (zh)
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CN108026591A (zh
Inventor
P·帕特尔
I·吴
A·理查德森
K·贝尔霍钦
J·李
S·塔巴克曼
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Theranos Inc
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Theranos Inc
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Priority claimed from PCT/US2014/056151 external-priority patent/WO2015076919A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580062485.XA 2014-09-17 2015-09-17 诊断方法和组合物 Active CN108026591B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110943909.0A CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201462051945P 2014-09-17 2014-09-17
US201462051912P 2014-09-17 2014-09-17
USPCT/US2014/056151 2014-09-17
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification
US62/051,912 2014-09-17
US62/051,945 2014-09-17
US201462068603P 2014-10-24 2014-10-24
US201462068605P 2014-10-24 2014-10-24
US62/068,603 2014-10-24
US62/068,605 2014-10-24
US201562151358P 2015-04-22 2015-04-22
US62/151,358 2015-04-22
PCT/US2015/050811 WO2016044664A1 (en) 2014-09-17 2015-09-17 Diagnostic methods and compositions

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202110943909.0A Division CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Publications (2)

Publication Number Publication Date
CN108026591A CN108026591A (zh) 2018-05-11
CN108026591B true CN108026591B (zh) 2021-09-07

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CN201580062485.XA Active CN108026591B (zh) 2014-09-17 2015-09-17 诊断方法和组合物
CN201580061362.4A Pending CN107002066A (zh) 2014-09-17 2015-09-17 复合式多步核酸扩增
CN202110943909.0A Pending CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物

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CN201580061362.4A Pending CN107002066A (zh) 2014-09-17 2015-09-17 复合式多步核酸扩增
CN202110943909.0A Pending CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Country Status (8)

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US (3) US9909193B2 (https=)
EP (2) EP3194629A4 (https=)
JP (6) JP2017532030A (https=)
CN (3) CN108026591B (https=)
BR (1) BR112017005392A2 (https=)
CA (2) CA2960859A1 (https=)
MX (2) MX2017003473A (https=)
WO (2) WO2016044664A1 (https=)

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CA2960859A1 (en) 2014-09-17 2016-03-24 Theranos, Inc. Hybrid multi-step nucleic acid amplification
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
BR112019012057A2 (pt) 2016-12-15 2019-11-12 Meharry Medical College agente antiviral, composição farmacêutica para a administração do mesmo e métodos de redução na replicação do vírus zika (zikv) e tratamento de doença mediada pelo vírus zika (zikv)
FI130673B1 (en) * 2020-11-24 2024-01-11 Mobidiag Oy Method and system for performing a cumulative nucleic acid amplification reaction

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US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
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EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
JP4517061B2 (ja) * 2003-08-08 2010-08-04 独立行政法人産業技術総合研究所 ダンベル型ｄｎａの効率的な製造方法
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Also Published As

Publication number Publication date
MX2017003473A (es) 2017-08-02
US20160201148A1 (en) 2016-07-14
US9909193B2 (en) 2018-03-06
JP2021000138A (ja) 2021-01-07
BR112017005392A2 (pt) 2017-12-12
MX366113B (es) 2019-06-26
EP3194590A4 (en) 2018-05-09
CN113736863A (zh) 2021-12-03
JP2023011943A (ja) 2023-01-24
CN108026591A (zh) 2018-05-11
EP3194590A1 (en) 2017-07-26
CN107002066A (zh) 2017-08-01
US20180223381A1 (en) 2018-08-09
JP2022110152A (ja) 2022-07-28
JP2017532030A (ja) 2017-11-02
EP3194629A4 (en) 2018-03-07
US20170342476A1 (en) 2017-11-30
JP2021006056A (ja) 2021-01-21
JP2017531424A (ja) 2017-10-26
MX2017003475A (es) 2017-08-02
WO2016044673A1 (en) 2016-03-24
EP3194629A1 (en) 2017-07-26
CA2960859A1 (en) 2016-03-24
CA2961209A1 (en) 2016-03-24
WO2016044664A1 (en) 2016-03-24

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