CN107980617A - A kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation - Google Patents
A kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation Download PDFInfo
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- CN107980617A CN107980617A CN201810045331.5A CN201810045331A CN107980617A CN 107980617 A CN107980617 A CN 107980617A CN 201810045331 A CN201810045331 A CN 201810045331A CN 107980617 A CN107980617 A CN 107980617A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention belongs to fruit tree hybridization breeding field, more particularly to a kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation, comprises the following steps：(1) scion culture；(2) stock culture；(3) it is tender to an old grafting；(4) pinching promotees to depart from juvenile phase by force；(5) long scion is to fruitful branch secondary grafting；(6) hybridization of lower generation and the identification of this generation fruit.The present invention, which innovatively integrates, applies " tender to old " grafting, the twice technology such as " pinching by force ", " long scion is to fruitful branch " secondary grafting, " castrating thorn " and " flower forcing room is induced into flower ", technical route, principle from have in cultivating technique flow with traditional Normal practice significant innovation with it is different, duration significant effect needed for shortening a generation cycle of crossbreeding, is the more excellent means of fruit tree selection cross kind.
Description
【Technical field】
It is more particularly to a kind of to shorten juvenile phase Rapid identification dragon fruit filial generation the invention belongs to fruit tree hybridization breeding field
Method.
【Background technology】
At present, in fruit breeding obtain filial generation seed after, Normal practice mainly directly planted by seedling or
Common grafting mode, allows filial generation plant to grow the regular hour naturally in crop field until it departs from juvenile phase, to yielding positive results
Carry out hybridization of future generation or fruit characterization test again afterwards.Directly planted by seedling or common grafting mode is cultivated, its
The time for departing from experience needed for juvenile phase to flowering naturally result first is longer.Common saying " Peach trees take three years to bear fruits and four years you will wait for apricots apricot 5 years ", what is said is plant
The time limit from seed sprouting to result, usual peach need 3 years, Japanese plum 4 years, apricot 5 years, and dragon fruit usually needs 24-36 months.
Therefore, identify that dragon fruit filial generation needs the very long time, result in slow to the progress of dragon fruit crossbreeding.
【The content of the invention】
For the difficult point of the above-mentioned prior art, it is an object of the invention to provide one kind to shorten juvenile phase Rapid identification dragon fruit
The method of filial generation, by shortening the juvenile phase of the fruit tree seedling such as dragon fruit, promotes filial generation seedling to be tied in advance into flower
Fruit carrys out the trait expression of Rapid identification filial generation, so as to achieve the purpose that to accelerate breeding；When overcoming with traditional conventional method, from
The shortcomings that time undergone needed for seedling growth to its disengaging juvenile phase to flowering naturally result first is longer.
To solve the above problems, the technical solution adopted in the present invention is：It is miscellaneous that one kind shortens juvenile phase Rapid identification dragon fruit
The method for handing over offspring, comprises the following steps：
(1) scion culture：Take dragon fruit filial generation seed to carry out vernalization to show money or valuables one carries unintentionally, then sow to long in nutrition cup
Into seedling, topping treatment is carried out when seedling length is to 2cm, the implant left it is aging it is stout and strong after can be used as step (3)
Scion；
(2) stock culture：The red imperial No. 1 plant progress in osmanthus is potted plant, the thorn seat on all descending branches of plant is excluded, in order to
Allow the branch that nutrition concentration dependency is grown upward slightly, when the branch grown upward slightly grows to 20cm, pinched and castrated thorn processing,
It can be used as the stock of step (3) after turning green；
(3) it is tender to an old grafting：The scion that step (1) obtains is taken to graft on the stock of step (2), grafting and healing
The optimum of culture is 20-25 DEG C of temperature, relative humidity 85-95%；
(4) pinching promotees to depart from juvenile phase by force：Potted plant go under the conditions of full exposure that step (3) is survived is cultivated, and is poured within every 3 days
The pancebrin of one time 0.1%, when a sprouting of the potted plant scion pumping after a grafting survival is grown to 1cm, by scion
On remaining thorn seat need to all castrate and remove, be carried out at the same time pinching topping treatment, only retain base portion 1-2 sections, in order to which secondary sprouting is taken out
It is raw；
Pinching topping treatment is carried out again when secondary sprouting is grown to 1cm, only retains base portion 1-2 section, in order to sprouting three times
Pumping；
When sprouting pumping is to 20-30cm three times, if being marked it was observed that piercing seat and becoming normal needle-shaped thorn seat via seta
Will seedling and has disengaged from juvenile phase, carries out pinching topping treatment at this time, and the sprouting three times after turning green becomes third generation branch；
(5) long scion is to fruitful branch secondary grafting：It is branch fringe to take the third generation branch 20-30cm that step (4) obtains, grafting
The descending branch end of plant in step (2), after surviving 20d, goes to flower forcing room and induces third generation branch into flower；
(6) hybridization of lower generation and the identification of this generation fruit：Third generation branch can carry out crossbreeding of future generation, the third generation when blooming
Fruit characterization test can be carried out after hybridization of the non-lower generation fruit maturation of branch.
The invention has the advantages that：
1st, the present invention " shortening juvenile phase Rapid identification fruit tree filial generation " method, innovatively integrates and applies " tender to old "
The technology such as grafting, twice " pinching by force ", " long scion is to fruitful branch " secondary grafting, " castrating thorn " and " flower forcing room induction into flower ",
In technical route, principle from having significant innovation in cultivating technique flow with traditional Normal practice with different, shortening crossbreeding
A generation cycle needed for duration significant effect, be fruit tree selection cross kind more excellent means.
2nd, the method for the shortening juvenile phase Rapid identification dragon fruit filial generation that the present invention uses, is applied in fruit trees such as dragon fruits
Crossbreeding on, generation cycle of dragon fruit crossbreeding can be made by being up to the free period of 24-36 months, contracting originally
It is as short as 8-10 months, saves the time of more than half.
3rd, the present invention shortens juvenile phase Rapid identification fruit tree filial generation, and standardization, unification, controllable degree are high, are easy to same
Lateral comparison between seeds different cultivars.
4th, the present invention shortens juvenile phase Rapid identification fruit tree filial generation, applies and is put down in the crossbreeding of the fruit trees such as dragon fruit
Single plant takes up an area less, easy to operate to save manually.
5th, the present invention has the innovation of substance in product appearance structure, effect compared with the similar product announced
And difference, there is the innovation of substance with the similar products announced in terms of the process method flow of production product and set
Meter.There is potential promote to be worth for innovation and progress to industry field.
【Brief description of the drawings】
Fig. 1 is the growth schematic diagram of stock culture of the embodiment of the present invention；
Fig. 2 is the tender growth schematic diagram to an old grafting of the embodiment of the present invention；
Fig. 3 is growth schematic diagram of the long scion of the embodiment of the present invention to fruitful branch secondary grafting.
【Embodiment】
Embodiment 1：
A kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation, comprises the following steps：
(1) scion culture：Take dragon fruit filial generation seed to carry out vernalization to show money or valuables one carries unintentionally, then sow to long in nutrition cup
Into seedling, topping treatment is carried out when seedling length is to 2cm, the implant left it is aging it is stout and strong after can be used as step (3)
Scion；
(2) stock culture：The red imperial No. 1 plant progress in osmanthus is potted plant, the thorn seat on all descending branches of plant is excluded, in order to
Allow the branch that nutrition concentration dependency is grown upward slightly, when the branch grown upward slightly grows to 20cm, pinched and castrated thorn processing,
It can be used as the stock of step (3) after turning green；
(3) it is tender to an old grafting：By the use of sharp cutter cut step (1) obtain implant 1.0cm as scion, using patch
Connection is grafted on the stock of step (2), is comprised the following steps that：1. cutter is got out, bandage, alcohol, the instrument such as cotton；
2. vertically indulging partial application along the xylem of rib at the top of stock with cutter, deep 3cm is then parallel to cut one again
Knife, by the seamed edge cut off a right angle, hold excision seamed edge loop friction 5 stock xylems, retain juice in incision；
3. taking the scion of 1cm long, the horizontal plane that scion lower cut is placed with to stock leans on xylem side；
4. the bandage of 5cm long is taken to fix scion；
5. castrate except institute's spinosity seat on stock；
Finally by the plant after grafting, it is positioned in the rain-shelter of 20 DEG C of temperature, relative humidity 85%, degree of shading 90%,
Carry out showing to survive as scion remains unchanged or slightly wilts after healing grown cultures 7d, 7d；Dry, the table if scion is wilted
Bright poor healing, can connect other scion in another seamed edge immediately；
(4) pinching promotees to depart from juvenile phase by force：Potted plant go under the conditions of full exposure that step (3) is survived is cultivated, and is poured within every 3 days
The pancebrin of one time 0.1%, when a sprouting of the potted plant scion pumping after a grafting survival is grown to 1cm, by scion
On remaining thorn seat need to all castrate and remove, be carried out at the same time pinching topping treatment, only retain base portion 1 and save, in order to secondary sprouting pumping；
Pinching topping treatment is carried out again when secondary sprouting is grown to 1cm, only retains base portion 1 and saves, in order to which sprouting is taken out three times
It is raw；
When sprouting pumping is to 20cm three times, if being indicated it was observed that piercing seat and becoming normal needle-shaped thorn seat via seta
Seedling has disengaged from juvenile phase, carries out pinching topping treatment at this time, and the sprouting three times after turning green becomes third generation branch；
(5) long scion is to fruitful branch secondary grafting：It is branch fringe to take the third generation branch 20cm that step (4) obtains, using slotting
Connection grafts in the descending branch end of plant in step (2), and concrete operation step is as follows：1. cutter is got out, bandage, wine
The instruments such as essence, cotton；
2. laterally cutting 2cm in stock end with secateurs, expose smooth notch；
3. colliding in the position of suitable order 1cm to medulla insertion blade until with medulla, edegewise side slowly rotates
Until exocuticle sees that stain occurs；
4. taking specific purpose tool to be inserted into along medulla to collide until with blade, 2 circles are rotated, sleeve is taken out and removes stem meat, clip
20cm scion, takes specific purpose tool alignment medulla insertion, and rotate a circle medulla needed for intercepting as scale with cutter incision medulla, will
Stock is inserted into scion, and scion is fixed with bandage；
5. castrate except institute's spinosity seat on stock；Plant after grafting is positioned over 20 DEG C of temperature, relative humidity 85%, degree of shading
In 90% rain-shelter, healing grown cultures 7d being carried out, sees whether to survive, potted plant after surviving is gone under the conditions of full exposure,
Every 3 days pancebrins for pouring one time 0.1%, culture go to flower forcing room for 2 months and induce third generation branch into flower；
(6) hybridization of lower generation and the identification of this generation fruit：Third generation branch can carry out crossbreeding of future generation, the third generation when blooming
Fruit characterization test can be carried out after hybridization of the non-lower generation fruit maturation of branch.
Embodiment 2：
A kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation, comprises the following steps：
(1) scion culture：Take dragon fruit filial generation seed to carry out vernalization to show money or valuables one carries unintentionally, then sow to long in nutrition cup
Into seedling, topping treatment is carried out when seedling length is to 2cm, the implant left it is aging it is stout and strong after can be used as step (3)
Scion；
(2) stock culture：The red imperial No. 1 plant progress in osmanthus is potted plant, the thorn seat on all descending branches of plant is excluded, in order to
Allow the branch that nutrition concentration dependency is grown upward slightly, when the branch grown upward slightly grows to 20cm, pinched and castrated thorn processing,
It can be used as the stock of step (3) after turning green；
(3) it is tender to an old grafting：By the use of sharp cutter cut step (1) obtain implant 1.0cm as scion, using patch
Connection is grafted on the stock of step (2), is comprised the following steps that：1. cutter is got out, bandage, alcohol, the instrument such as cotton；
2. vertically indulging partial application along the xylem of rib at the top of stock with cutter, deep 3.5cm is then parallel to cut one again
Knife, by the seamed edge cut off a right angle, hold excision seamed edge loop friction 5 stock xylems, retain juice in incision；
3. taking the scion of 1cm long, the horizontal plane that scion lower cut is placed with to stock leans on xylem side；
4. the bandage of 5.5cm long is taken to fix scion；
5. castrate except institute's spinosity seat on stock；
Finally by the plant after grafting, it is positioned in the rain-shelter of 22 DEG C of temperature, relative humidity 88%, degree of shading 90%,
Carry out showing to survive as scion remains unchanged or slightly wilts after healing grown cultures 7d, 7d；Dry, the table if scion is wilted
Bright poor healing, can connect other scion in another seamed edge immediately；
(4) pinching promotees to depart from juvenile phase by force：Potted plant go under the conditions of full exposure that step (3) is survived is cultivated, and is poured within every 3 days
The pancebrin of one time 0.1%, when a sprouting of the potted plant scion pumping after a grafting survival is grown to 1cm, by scion
On remaining thorn seat need to all castrate and remove, be carried out at the same time pinching topping treatment, only retain base portion 1.5 and save, in order to which secondary sprouting is taken out
It is raw；
Pinching topping treatment is carried out again when secondary sprouting is grown to 1cm, is only retained base portion 1.5 and is saved, in order to sprouting three times
Pumping；
When sprouting pumping is to 20cm three times, if being indicated it was observed that piercing seat and becoming normal needle-shaped thorn seat via seta
Seedling has disengaged from juvenile phase, carries out pinching topping treatment at this time, and the sprouting three times after turning green becomes third generation branch；
(5) long scion is to fruitful branch secondary grafting：It is branch fringe to take the third generation branch 25cm that step (4) obtains, using slotting
Connection grafts in the descending branch end of plant in step (2), and concrete operation step is as follows：1. cutter is got out, bandage, wine
The instruments such as essence, cotton；
2. laterally cutting 2.2cm in stock end with secateurs, expose smooth notch；
3. colliding in the position of suitable order 1.4cm to medulla insertion blade until with medulla, edegewise side slowly turns
Move until exocuticle sees that stain occurs；
4. taking specific purpose tool to be inserted into along medulla to collide until with blade, 2 circles are rotated, sleeve is taken out and removes stem meat, clip
26cm scion, takes specific purpose tool alignment medulla insertion, and rotate a circle medulla needed for intercepting as scale with cutter incision medulla, will
Stock is inserted into scion, and scion is fixed with bandage；
5. castrate except institute's spinosity seat on stock；Plant after grafting is positioned over 23 DEG C of temperature, relative humidity 90%, degree of shading
In 90% rain-shelter, healing grown cultures 7d being carried out, sees whether to survive, potted plant after surviving is gone under the conditions of full exposure,
Every 3 days pancebrins for pouring one time 0.1%, culture go to flower forcing room for 2 months and induce third generation branch into flower；
(6) hybridization of lower generation and the identification of this generation fruit：Third generation branch can carry out crossbreeding of future generation, the third generation when blooming
Fruit characterization test can be carried out after hybridization of the non-lower generation fruit maturation of branch.
Embodiment 3：
A kind of method for shortening juvenile phase Rapid identification dragon fruit filial generation, comprises the following steps：
(1) scion culture：Take dragon fruit filial generation seed to carry out vernalization to show money or valuables one carries unintentionally, then sow to long in nutrition cup
Into seedling, topping treatment is carried out when seedling length is to 2cm, the implant left it is aging it is stout and strong after can be used as step (3)
Scion；
(2) stock culture：The red imperial No. 1 plant progress in osmanthus is potted plant, the thorn seat on all descending branches of plant is excluded, in order to
Allow the branch that nutrition concentration dependency is grown upward slightly, when the branch grown upward slightly grows to 20cm, pinched and castrated thorn processing,
It can be used as the stock of step (3) after turning green；
(3) it is tender to an old grafting：By the use of sharp cutter cut step (1) obtain implant 1.0cm as scion, using patch
Connection is grafted on the stock of step (2), is comprised the following steps that：1. cutter is got out, bandage, alcohol, the instrument such as cotton；
2. vertically indulging partial application along the xylem of rib at the top of stock with cutter, deep 5cm is then parallel to cut one again
Knife, by the seamed edge cut off a right angle, hold excision seamed edge loop friction 5 stock xylems, retain juice in incision；
3. taking the scion of 1cm long, the horizontal plane that scion lower cut is placed with to stock leans on xylem side；
4. the bandage of 6cm long is taken to fix scion；
5. castrate except institute's spinosity seat on stock；
Finally by the plant after grafting, it is positioned in the rain-shelter of 25 DEG C of temperature, relative humidity 95%, degree of shading 90%,
Carry out showing to survive as scion remains unchanged or slightly wilts after healing grown cultures 7d, 7d；Dry, the table if scion is wilted
Bright poor healing, can connect other scion in another seamed edge immediately；
(4) pinching promotees to depart from juvenile phase by force：Potted plant go under the conditions of full exposure that step (3) is survived is cultivated, and is poured within every 3 days
The pancebrin of one time 0.1%, when a sprouting of the potted plant scion pumping after a grafting survival is grown to 1cm, by scion
On remaining thorn seat need to all castrate and remove, be carried out at the same time pinching topping treatment, only retain base portion 2 and save, in order to secondary sprouting pumping；
Pinching topping treatment is carried out again when secondary sprouting is grown to 1cm, only retains base portion 2 and saves, in order to which sprouting is taken out three times
It is raw；
When sprouting pumping is to 20cm three times, if being indicated it was observed that piercing seat and becoming normal needle-shaped thorn seat via seta
Seedling has disengaged from juvenile phase, carries out pinching topping treatment at this time, and the sprouting three times after turning green becomes third generation branch；
(5) long scion is to fruitful branch secondary grafting：It is branch fringe to take the third generation branch 30cm that step (4) obtains, using slotting
Connection grafts in the descending branch end of plant in step (2), and concrete operation step is as follows：1. cutter is got out, bandage, wine
The instruments such as essence, cotton；
2. laterally cutting 3cm in stock end with secateurs, expose smooth notch；
3. colliding in the position of suitable order 1.5cm to medulla insertion blade until with medulla, edegewise side slowly turns
Move until exocuticle sees that stain occurs；
4. taking specific purpose tool to be inserted into along medulla to collide until with blade, 2 circles are rotated, sleeve is taken out and removes stem meat, clip
30cm scion, takes specific purpose tool alignment medulla insertion, and rotate a circle medulla needed for intercepting as scale with cutter incision medulla, will
Stock is inserted into scion, and scion is fixed with bandage；
5. castrate except institute's spinosity seat on stock；Plant after grafting is positioned over 25 DEG C of temperature, relative humidity 95%, degree of shading
In 90% rain-shelter, healing grown cultures 7d being carried out, sees whether to survive, potted plant after surviving is gone under the conditions of full exposure,
Every 3 days pancebrins for pouring one time 0.1%, culture go to flower forcing room for 2 months and induce third generation branch into flower；
(6) hybridization of lower generation and the identification of this generation fruit：Third generation branch can carry out crossbreeding of future generation, the third generation when blooming
Fruit characterization test can be carried out after hybridization of the non-lower generation fruit maturation of branch.
Experiment 1：Using the pith-cambium grafting method in above-mentioned specific embodiment 1, scion note is connected on the stock in red imperial No. 1 of osmanthus, had
Body parameter reference embodiment 1, repeats experiment 3 times, calculates the average survival after note connects, and concrete outcome is shown in Table 1.
Table 1：
As shown in Table 1：Either " red imperial No. 2 of osmanthus " still " U.S. dragon No. 1 " kind, its note are connected on " red imperial No. 1 of osmanthus "
Average survival reaches 80.0%, and survival rate is high.
Experiment 2：Using the method for plant propagation with cutting in above-mentioned specific embodiment 1, scion note is connected on the stock in red imperial No. 1 of osmanthus, had
Body parameter reference embodiment 1, calculates the average survival after the method for plant propagation with cutting, and concrete outcome is shown in Table 2.
Table 2：
As shown in Table 2：Either " red imperial No. 2 of osmanthus " still " U.S. dragon No. 1 " kind, it is plugged on " red imperial No. 1 of osmanthus "
Average survival reaches more than 85.0%, and survival rate is high.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, equal change or the modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (1)
- A kind of 1. method for shortening juvenile phase Rapid identification dragon fruit filial generation, it is characterised in that：Comprise the following steps：(1) scion culture：Take dragon fruit filial generation seed to carry out vernalization to show money or valuables one carries unintentionally, then sow to growing up to reality in nutrition cup Raw seedling, topping treatment is carried out when seedling length is to 2cm, the implant left it is aging it is stout and strong after can be used as the scion of step (3)；(2) stock culture：The red imperial No. 1 plant progress in osmanthus is potted plant, the thorn seat on all descending branches of plant is excluded, is sought in order to allow The branch that foster concentration dependency is grown upward slightly, when the branch grown upward slightly grows to 20cm, is pinched and is castrated thorn processing, turned green It can be used as the stock of step (3) afterwards；(3) it is tender to an old grafting：The scion that step (1) obtains is taken to graft on the stock of step (2), grafting and healing are cultivated Optimum for 20-25 DEG C of temperature, relative humidity 85-95%；(4) pinching promotees to depart from juvenile phase by force：Potted plant go under the conditions of full exposure that step (3) is survived is cultivated, and is poured once within every 3 days 0.1% pancebrin, when a sprouting of the potted plant scion pumping after a grafting survival is grown to 1cm, by scion Remaining thorn seat, which need to be castrated all, to be removed, and is carried out at the same time pinching topping treatment, is only retained base portion 1-2 sections, in order to secondary sprouting pumping；Pinching topping treatment is carried out again when secondary sprouting is grown to 1cm, only retains base portion 1-2 section, in order to sprouting pumping three times；When sprouting pumping is to 20-30cm three times, if being indicated it was observed that piercing seat and becoming normal needle-shaped thorn seat via seta Seedling has disengaged from juvenile phase, carries out pinching topping treatment at this time, and the sprouting three times after turning green becomes third generation branch；(5) long scion is to fruitful branch secondary grafting：It is branch fringe to take the third generation branch 20-30cm that step (4) obtains, and grafts in step Suddenly in (2) plant descending branch end, after surviving 20d, go to flower forcing room and induce third generation branch into flower；(6) hybridization of lower generation and the identification of this generation fruit：Third generation branch can carry out crossbreeding of future generation, the third generation branch when blooming Non- lower generation hybridization fruit maturation after can carry out fruit characterization test.
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| CN109197204A (en) * | 2018-08-28 | 2019-01-15 | 华亭县正元生态农业发展有限公司 | A kind of breeding method of high winter resistance dragon fruit new varieties |
| CN109924076A (en) * | 2019-03-25 | 2019-06-25 | 广西壮族自治区农业科学院园艺研究所 | A kind of breeding method shortening the dragon fruit crossbreeding period |
| CN111386879A (en) * | 2020-05-06 | 2020-07-10 | 罗有海 | In-tree culture method of dragon fruit fattening stock |
| CN112568073A (en) * | 2020-12-14 | 2021-03-30 | 广西壮族自治区农业科学院 | Cultivation method for dragon fruit seedlings |
| CN112772171A (en) * | 2021-02-08 | 2021-05-11 | 福建省农业科学院果树研究所 | Method for increasing number of waxberry hybrid progeny |
| CN112790062A (en) * | 2021-02-26 | 2021-05-14 | 中国热带农业科学院海口实验站 | Multi-stage pruning method for passion fruit seedlings |
| CN114514829A (en) * | 2022-03-08 | 2022-05-20 | 中国热带农业科学院热带作物品种资源研究所 | Method for increasing grafting survival rate of tender buds of pitaya |
| CN114568140A (en) * | 2022-03-08 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Cultivation method of high-quality dragon fruit stock |
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| CN109197204A (en) * | 2018-08-28 | 2019-01-15 | 华亭县正元生态农业发展有限公司 | A kind of breeding method of high winter resistance dragon fruit new varieties |
| CN109924076A (en) * | 2019-03-25 | 2019-06-25 | 广西壮族自治区农业科学院园艺研究所 | A kind of breeding method shortening the dragon fruit crossbreeding period |
| CN109924076B (en) * | 2019-03-25 | 2022-01-04 | 广西壮族自治区农业科学院园艺研究所 | Breeding method for shortening dragon fruit crossbreeding period |
| CN111386879A (en) * | 2020-05-06 | 2020-07-10 | 罗有海 | In-tree culture method of dragon fruit fattening stock |
| CN111386879B (en) * | 2020-05-06 | 2022-05-27 | 罗有海 | In-tree culture method of dragon fruit fattening stock |
| CN112568073A (en) * | 2020-12-14 | 2021-03-30 | 广西壮族自治区农业科学院 | Cultivation method for dragon fruit seedlings |
| CN112772171A (en) * | 2021-02-08 | 2021-05-11 | 福建省农业科学院果树研究所 | Method for increasing number of waxberry hybrid progeny |
| CN112790062A (en) * | 2021-02-26 | 2021-05-14 | 中国热带农业科学院海口实验站 | Multi-stage pruning method for passion fruit seedlings |
| CN114514829A (en) * | 2022-03-08 | 2022-05-20 | 中国热带农业科学院热带作物品种资源研究所 | Method for increasing grafting survival rate of tender buds of pitaya |
| CN114568140A (en) * | 2022-03-08 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Cultivation method of high-quality dragon fruit stock |
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