CN107969384A - A kind of cultural method of the emerald green duck of dragon victory - Google Patents
A kind of cultural method of the emerald green duck of dragon victory Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/55—Linaceae (Flax family), e.g. Linum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to cultural technique field, more particularly to a kind of cultural method of the emerald green duck of dragon victory, operate in accordance with the following steps:For the duckling in 36 week old, the feed configured according to the raw material of following mass percentage is fed, 3 times/day, and 80 200g/ every time, and by its free choice feeding to all you can eat;The composition of the feed is as follows:Corn protein powder 50 60%, the coconut dregs of rice 10 15%, mung bean flour 8 15%, maize alcohol lees 5 8%, steam fish meal 2 5%, salt 1 3%, calcium monohydrogen phosphate 3 5%, disease-resistant auxiliary material 2 5%.Advantage of the present invention:The feed for finding to feed the application in periodic time by substantial amounts of research can not only improve growth rate, also have the effect for suppressing virus well, so that the survival rate of the emerald green duck of dragon victory is improved, and then the purpose increased income.
Description
Technical field
The present invention relates to cultural technique field, more particularly to a kind of cultural method of the emerald green duck of dragon victory.
Background technology
Dragon wins emerald green duck, and the emerald green duck of dragon victory is blackish green because its whole body feather black carries, and gains the name in the gloss as emerald.
Main product is also named " foreign hole duck ", means the duck that people from Miao ethnic group supports in Miao ethnic group compatriot residence, locality.It is mainly distributed on
Guangxi Zhuang Autonomous Region Long Shengxian Ma Di, big Jiang Xiang.According to records, " foreign hole duck " feather black bands bird with red feathers, produces green-shell egg, gregariousness
It is good, it is suitable for the water surface and rice field is put in a suitable place to breed.The emerald green duck of dragon victory has unique shape and appearance, has high ornamental value, and meat is thin
Tender no fishy smell, is the rare genetic resources of duck varieties.
The content of the invention
The goal of the invention of the present invention is:A kind of cultural method of the emerald green duck of dragon victory is provided, is found by substantial amounts of research
The feed that periodic time feeds the application can not only improve growth rate, also have the effect for suppressing virus well, so as to carry
The survival rate of the high emerald green duck of dragon victory, and then the purpose increased income.
In order to realize foregoing invention purpose, the technical solution adopted by the present invention is as follows:
A kind of cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps:
For the duckling in 3-6 week old, the feed configured according to the raw material of following mass percentage is fed,
3 times/day, and 80-200g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 50-60%, coconut dregs of rice 10-15%, mung bean flour 8-15%, corn
Alcohol grain 5-8%, steam fish meal 2-5%, salt 1-3%, calcium monohydrogen phosphate 3-5%, disease-resistant auxiliary material 2-5%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:25%-35% Radix Curcumae extracts, 15%-
25% Semen Lini extract, 15%-20% mulberry bark extracts, 15%-20% common adenostema herbs extract, 5%-15% rhizoma imperataes carry
Take thing, 5%-10% dried rehamnnia root extracts;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After the 10-30% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, dope are added:Sweetener:Methylol is fine
The plain weight ratio of dimension is 10:1-2:2-3, after being followed by stirring for uniformly, is put into dryer and is dried, and is using power while drying
50-80W, frequency are modified processing for 30-50 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the common adenostema herb extract is prepared by following methods:Common adenostema herb drying and crushing is crossed into screening
The common adenostema herb powder that particle diameter is 1-2 microns is taken, the ethanol solution that the mass fraction for adding 5-10 times is 50-60% places conical flask
In, water-bath extraction is carried out under the conditions of the use of bath temperature being 30-40 DEG C, water-bath uses power as 100-150W, frequency during extracting
Rate is 40-80 hertz of ultrasonic wave assisted extractions, and water-bath extraction time is 40-55 minutes, is centrifuged while hot, the bar of centrifugation
Part is 2500-3000r/min, and the time of centrifugation is 30-50s, takes filtrate to be then concentrated under reduced pressure.In obtained material
Content containing organic acid is 1.62-5.36mg/g, the content of alkane type Diterpenes is 0.95-2.67mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the Radix Curcumae extract is prepared by following methods:Picking is taken in the noon on the 15th of August in 2016
The rhizome of 12 points of Radix Curcumae, crosses 50-100 mesh sieves, weighs 50-80g, put in boiling flask, add after dry, pulverize after removal of impurities
Enter mass concentration be 75-80% ethanol solution 250-350mL, carry out alcohol extracting, in alcohol extracting also using ultrasonic power for 100W,
Frequency is 40 hertz of assisted extractions 20-25 minute, takes out standing to room temperature, filters to take filtrate and then carry out being concentrated under reduced pressure into substance
Long-pending 1.01 times.In obtained material the content containing zingiberene be 0.67mg/g-1.26mg/g, flavones content be
1.15mg/g-3.25mg/g。
Further illustrate, the Semen Lini extract is prepared by following methods:After linseed is crushed, add compound
Enzyme hydrolysis 10-20 minutes, carries out 3500-4000r/min using centrifuge and centrifuges 5-10 minutes, take supernatant, add Sevag examinations
Agent takes off albumen 2-3 times, each 5-8 minutes, then carries out after being concentrated in vacuo to 1.12 times of original volume, adds mass fraction and is
The ethanol solution of 70-80% carries out alcohol precipitation at 5-8 DEG C and stays overnight, and then carries out 3000-4000r/min centrifugations 1- using centrifuge
3 minutes, precipitation is taken out after standing, extract is obtained after steam drying;The Sevag reagents be according to volume ratio chloroform-
N-butanol 3-4: 1 is mixed to get;Contain linolenic content in obtained material for 1.24mg/g-3.15mg/g, lignanoids
The content of compound is 0.36mg/g-1.64mg/g.
Further illustrate, the mulberry bark extract is prepared by following methods:After root bark of white mulberry rhizome clean dry,
50-100 mesh sieves are crushed, 100-120g powder is weighed and adds in conical flask, add the volume fraction 40-50% ethanol of 500mL
Solution, the mass concentration for being subsequently added into 10mL are the ammonium sulfate of 0.3-0.4g/m L, and vibration stands 20-25 points after 1 minute
Clock, 40-50 DEG C of temperature, power 50-60W ultrasound condition under extract 30min, filter, will solution move into separatory funnel in it is quiet
Put layering.Upper solution is taken, mulberry bark extract is can obtain after being spray-dried.Containing containing flavones in obtained material
The content measured as 1.06-2.31mg/g, sterol is 0.96-1.25mg/g.
Further illustrate, the cogongrass rhizome extractive is prepared by following methods:The rhizoma imperatae of autumn picking is crushed
After take 200-300g to add in triangular flask, the acetic acid methanol solution of the 1-3% of cumulative volume is added, at 35-40 DEG C, microwave power
The reaction of 20-25 minutes is carried out under 500W, is then centrifuged and takes solution, rhizoma imperatae extraction is can obtain after spray drying
Thing.Contain Cylindrin 1.21mg/g-2.64mg/g, arundoin 1.13mg/g-2.15mg/g in obtained material.
Further illustrate, the dried rehamnnia root extract is prepared by following methods:After dried rehamnnia root remove seed is smashed to pieces, add
Mass fraction 40-50% ethanol solutions carry out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2-3 times, each 20-30 points
Clock, the temperature of extraction is 50-60 DEG C, be concentrated under reduced pressure into 1.12 times of original volume after the filtrate for repeating extraction is merged
Obtain dried rehamnnia root extract;The volume ratio of dried rehamnnia root and ethanol solution is 1:10-25.Contain alkaloid in obtained material
1.21-2.67mg/g, martynoside 0.86mg/g-1.96mg/g.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows:
It is corn protein powder of the present invention, the coconut dregs of rice, mung bean flour, maize alcohol lees, steam fish meal, salt, calcium monohydrogen phosphate, disease-resistant
Auxiliary material has reached after proportioning contains organic matter 50.21-56.23%, trace element 11.26-14.32%, effective carbon
19.63-24.36%, effective calcium 15.63-20.26% can meet the emerald green duck nutrient elements required for the growth of dragon victory, promote growth, and
And with the addition of disease-resistant auxiliary material has the function that good anti-hepatitis, the survival rate of the emerald green duck of dragon victory is improved.
The effect of Chinese medicine active compound:Radix Curcumae is cold in nature, acrid flavour, hardship.Return liver warp, the heart channel of Hang-Shaoyin, lung channel.It is promoting blood circulation and stopping pain, promoting the circulation of qi scabies, clear
Heart solution is strongly fragrant, normalizing gallbladder to cure jaundice.Common adenostema herb is pungent, slight bitter, cool;Return lung, Liver Channel;It is clearing heat and detoxicating, dispelling wind and eliminating dampness.Linseed is sweet in flavor, mild-natured.
Distributed in lung channel, Liver Channel, large intestine channel.Moisturize, wind-dispelling.The root bark of white mulberry is cold in nature, sweet in flavor;It is distributed in lung channel;Removing heat from lung and relieving asthma, inducing diuresis for removing edema.Rhizoma imperatae
It is sweet, cold;Enter lung, stomach, small intestinl channel;Cool blood, hemostasis, heat-clearing, diuresis.Dried rehamnnia root sweetness and bitterness, it is cool;Enter the heart, liver and kidney channel;Enriching yin, is supported
Blood.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.This specification (including any accessory claim, summary)
Disclosed in any feature, unless specifically stated, can be replaced by other alternative features that are equivalent or have similar purpose.
I.e., unless specifically stated, each feature is an example in a series of equivalent or similar characteristics.
Embodiment 1
A kind of cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps:
For the duckling in 3 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 80g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 60%, the coconut dregs of rice 15%, mung bean flour 12%, maize alcohol lees
5%th, steam fish meal 2%, salt 1%, calcium monohydrogen phosphate 3%, disease-resistant auxiliary material 2%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:35% Radix Curcumae extract, 25% linseed
Extract, 15% mulberry bark extract, 15% common adenostema herb extract, 5% cogongrass rhizome extractive, 5% dried rehamnnia root extract;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 10% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, dope are added:Sweetener:Hydroxymethyl cellulose
Weight ratio is 10:1:2, after being followed by stirring for uniformly, it is put into dryer and is dried, the use of power is 50W while drying, frequency
Processing is modified for 30 hertz of ultrasonic waves, you can obtains powdery disease-resistant auxiliary material.
Further illustrate, the common adenostema herb extract is prepared by following methods:Common adenostema herb drying and crushing is crossed into screening
The common adenostema herb powder that particle diameter is 1 micron is taken, the ethanol solution that the mass fraction for adding 5 times is 50% is placed in conical flask, is used
Bath temperature carries out water-bath extraction under the conditions of being 30 DEG C, water-bath extract during the use of power is 100W, frequency be 40 hertz of ultrasounds
Ripple assisted extraction, water-bath extraction time are 40 minutes, are centrifuged while hot, the condition of centrifugation is 2500r/min, centrifugation
Time is 30s, takes filtrate to be then concentrated under reduced pressure.In obtained material the content containing organic acid for 1.62mg/g,
The content of alkane type Diterpenes is 0.95mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the Radix Curcumae extract is prepared by following methods:Picking is taken in the noon on the 15th of August in 2016
The rhizome of 12 points of Radix Curcumae, crosses 50 mesh sieves, weighs 50g, put in boiling flask after dry, pulverize after removal of impurities, add quality
Concentration is 75% ethanol solution 250mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz
Assisted extraction 20 minutes, takes out and stands to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.
In obtained material the content containing zingiberene be 0.67mg/g, flavones content 1.15mg/g.
Further illustrate, the Semen Lini extract is prepared by following methods:After linseed is crushed, add compound
Enzyme hydrolysis 10 minutes, carries out 3500r/min using centrifuge and centrifuges 5 minutes, take supernatant, add Sevag reagents and take off albumen 2
It is secondary, 5 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 70%
Carry out alcohol precipitation at 5 DEG C to stay overnight, then carrying out 3000r/min using centrifuge centrifuges 1 minute, and precipitation, steam are taken out after standing
Extract is obtained after drying;The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3: 1;Obtained thing
Containing linolenic content be 1.24mg/g in matter, the content of Lignanoids compounds be 0.36mg/g.
Further illustrate, the mulberry bark extract is prepared by following methods:After root bark of white mulberry rhizome clean dry,
50 mesh sieves are crushed, 100g powder is weighed and adds in conical flask, add 40% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.3g/m L, and vibration stands 20 minutes after 1 minute, in 40 DEG C of temperature, power
30min is extracted under the ultrasound condition of 50W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Mulberry bark extract is can obtain after mist drying.The content containing flavones is 1.06mg/g, the content of sterol in obtained material
For 0.96mg/g.
Further illustrate, the cogongrass rhizome extractive is prepared by following methods:The rhizoma imperatae of autumn picking is crushed
After take 200g to add in triangular flask, add 1% acetic acid methanol solution of cumulative volume, at 35 DEG C, under microwave power 500W into
The row reaction of 20 minutes, is then centrifuged and takes solution, and cogongrass rhizome extractive is can obtain after spray drying.Obtained thing
Contain Cylindrin 1.21mg/g, arundoin 1.13mg/g in matter.
Further illustrate, the dried rehamnnia root extract is prepared by following methods:After dried rehamnnia root remove seed is smashed to pieces, add
40% ethanol solution of mass fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2 times, 20 minutes every time, extraction
Temperature be 50 DEG C, be concentrated under reduced pressure into 1.12 times of original volume after the filtrate for repeating extraction is merged can obtain dried rehamnnia root
Extract;The volume ratio of dried rehamnnia root and ethanol solution is 1:20.Contain alkaloid 1.21mg/g, martynoside in obtained material
0.86mg/g。。
Embodiment 2
A kind of cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps:
For the duckling in 5 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 100g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 59%, the coconut dregs of rice 15%, mung bean flour 8%, maize alcohol lees 6%,
Steam fish meal 3%, salt 2%, calcium monohydrogen phosphate 4%, disease-resistant auxiliary material 3%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:25% Radix Curcumae extract, 15% linseed
Extract, 15% mulberry bark extract, 20% common adenostema herb extract, 15% cogongrass rhizome extractive, 10% dried rehamnnia root extract;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 30% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, dope are added:Sweetener:Hydroxymethyl cellulose
Weight ratio is 10:2:3, after being followed by stirring for uniformly, it is put into dryer and is dried, the use of power is 80W while drying, frequency
Processing is modified for 50 hertz of ultrasonic waves, you can obtains powdery disease-resistant auxiliary material.
Further illustrate, the common adenostema herb extract is prepared by following methods:Common adenostema herb drying and crushing is crossed into screening
The common adenostema herb powder that particle diameter is 2 microns is taken, the ethanol solution that the mass fraction for adding 10 times is 60% is placed in conical flask, is used
Bath temperature carries out water-bath extraction under the conditions of being 40 DEG C, water-bath extract during the use of power is 150W, frequency be 80 hertz of ultrasounds
Ripple assisted extraction, water-bath extraction time are 55 minutes, are centrifuged while hot, the condition of centrifugation is 3000r/min, centrifugation
Time is 50s, takes filtrate to be then concentrated under reduced pressure.In obtained material the content containing organic acid for 2.34mg/g,
The content of alkane type Diterpenes is 1.17mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the Radix Curcumae extract is prepared by following methods:Picking is taken in the noon on the 15th of August in 2016
The rhizome of 12 points of Radix Curcumae, crosses 100 mesh sieves, weighs 80g, put in boiling flask after dry, pulverize after removal of impurities, add quality
Concentration is 80% ethanol solution 350mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz
Assisted extraction 25 minutes, takes out and stands to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.
In obtained material the content containing zingiberene be 0.94mg/g, flavones content 2.36mg/g.
Further illustrate, the Semen Lini extract is prepared by following methods:After linseed is crushed, add compound
Enzyme hydrolysis 20 minutes, carries out 4000r/min using centrifuge and centrifuges 10 minutes, take supernatant, add Sevag reagents and take off albumen 3
It is secondary, 8 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 80%
Carry out alcohol precipitation at 8 DEG C to stay overnight, then carrying out 4000r/min using centrifuge centrifuges 3 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying;The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 4: 1;Obtained thing
Containing linolenic content be 1.96mg/g in matter, the content of Lignanoids compounds be 0.69mg/g.
Further illustrate, the mulberry bark extract is prepared by following methods:After root bark of white mulberry rhizome clean dry,
100 mesh sieves are crushed, 120g powder is weighed and adds in conical flask, add 50% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.4g/mL, and vibration stands 25 minutes after 1 minute, in temperature 50 C, power
30min is extracted under the ultrasound condition of 60W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Mulberry bark extract is can obtain after mist drying.The content containing flavones is 1.38mg/g, the content of sterol in obtained material
For 1.02mg/g.
Further illustrate, the cogongrass rhizome extractive is prepared by following methods:The rhizoma imperatae of autumn picking is crushed
After take 300g to add in triangular flask, add 3% acetic acid methanol solution of cumulative volume, at 40 DEG C, under microwave power 500W into
The row reaction of 25 minutes, is then centrifuged and takes solution, and cogongrass rhizome extractive is can obtain after spray drying.Obtained thing
Contain Cylindrin 1.95mg/g, arundoin 1.67mg/g in matter.
Further illustrate, the dried rehamnnia root extract is prepared by following methods:After dried rehamnnia root remove seed is smashed to pieces, add
50% ethanol solution of mass fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 3 times, 30 minutes every time, extraction
Temperature be 60 DEG C, be concentrated under reduced pressure into 1.12 times of original volume after the filtrate for repeating extraction is merged can obtain dried rehamnnia root
Extract;The volume ratio of dried rehamnnia root and ethanol solution is 1:25.Contain alkaloid 1.84mg/g, martynoside in obtained material
1.12mg/g。
Embodiment 3
A kind of cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps:
For the duckling in 6 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 120g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 52%, the coconut dregs of rice 12%, mung bean flour 15%, maize alcohol lees
7%th, steam fish meal 4%, salt 2%, calcium monohydrogen phosphate 4%, disease-resistant auxiliary material 4%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:26% Radix Curcumae extract, 16% linseed
Extract, 20% mulberry bark extract, 18% common adenostema herb extract, 12% cogongrass rhizome extractive, 8% dried rehamnnia root extract;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 20% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, dope are added:Sweetener:Hydroxymethyl cellulose
Weight ratio is 10:1:3, after being followed by stirring for uniformly, it is put into dryer and is dried, the use of power is 60W while drying, frequency
Processing is modified for 35 hertz of ultrasonic waves, you can obtains powdery disease-resistant auxiliary material.
Further illustrate, the common adenostema herb extract is prepared by following methods:Common adenostema herb drying and crushing is crossed into screening
The common adenostema herb powder that particle diameter is 1.5 microns is taken, the ethanol solution that the mass fraction for adding 7 times is 53% is placed in conical flask, is made
Carries out water-bath extraction under the conditions of being 34 DEG C with bath temperature, water-bath extract during the use of power is 125W, frequency is 50 hertz and surpasses
Sound wave assisted extraction, water-bath extraction time are 45 minutes, are centrifuged while hot, and the condition of centrifugation is 2650r/min, centrifugation
Time be 35, take filtrate to be then concentrated under reduced pressure.In obtained material the content containing organic acid for 4.20mg/g,
The content of alkane type Diterpenes is 2.04mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the Radix Curcumae extract is prepared by following methods:Picking is taken in the noon on the 15th of August in 2016
The rhizome of 12 points of Radix Curcumae, crosses 60 mesh sieves, weighs 60g, put in boiling flask after dry, pulverize after removal of impurities, add quality
Concentration is 76% ethanol solution 300mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz
Assisted extraction 23 minutes, takes out and stands to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.
In obtained material the content containing zingiberene be 1.13mg/g, flavones content 2.34mg/g.
Further illustrate, the Semen Lini extract is prepared by following methods:After linseed is crushed, add compound
Enzyme hydrolysis 18 minutes, carries out 3600r/min using centrifuge and centrifuges 7 minutes, take supernatant, add Sevag reagents and take off albumen 2
It is secondary, 7 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 72%
Carry out alcohol precipitation at 6 DEG C to stay overnight, then carrying out 3800r/min using centrifuge centrifuges 2 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying;The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 4: 1;Obtained thing
Containing linolenic content be 2.05mg/g in matter, the content of Lignanoids compounds be 1.20mg/g.
Further illustrate, the mulberry bark extract is prepared by following methods:After root bark of white mulberry rhizome clean dry,
70 mesh sieves are crushed, 110g powder is weighed and adds in conical flask, add 43% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.35g/mL, and vibration stands 23 minutes after 1 minute, in 47 DEG C of temperature, power
30min is extracted under the ultrasound condition of 53W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Mulberry bark extract is can obtain after mist drying.The content containing flavones is 1.95mg/g, the content of sterol in obtained material
For 1.10mg/g.
Further illustrate, the cogongrass rhizome extractive is prepared by following methods:The rhizoma imperatae of autumn picking is crushed
After take 250g to add in triangular flask, add 2% acetic acid methanol solution of cumulative volume, at 38 DEG C, under microwave power 500W into
The row reaction of 22 minutes, is then centrifuged and takes solution, and cogongrass rhizome extractive is can obtain after spray drying.Obtained thing
Contain Cylindrin 2.36mg/g, arundoin 2.01mg/g in matter.
Further illustrate, the dried rehamnnia root extract is prepared by following methods:After dried rehamnnia root remove seed is smashed to pieces, add
43% ethanol solution of mass fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2 times, 28 minutes every time, extraction
Temperature be 55 DEG C, be concentrated under reduced pressure into 1.12 times of original volume after the filtrate for repeating extraction is merged can obtain dried rehamnnia root
Extract;The volume ratio of dried rehamnnia root and ethanol solution is 1:10.Contain alkaloid 2.01mg/g, martynoside in obtained material
1.53mg/g。
Embodiment 4
A kind of cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps:
For the duckling in 4 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 180g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 50%, the coconut dregs of rice 10%, mung bean flour 14%, maize alcohol lees
8%th, steam fish meal 5%, salt 3%, calcium monohydrogen phosphate 5%, disease-resistant auxiliary material 5%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:30% Radix Curcumae extract, 20% linseed
Extract, 16% mulberry bark extract, 17% common adenostema herb extract, 10% cogongrass rhizome extractive, 7% dried rehamnnia root extract;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 25% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, dope are added:Sweetener:Hydroxymethyl cellulose
Weight ratio is 10:2:3, after being followed by stirring for uniformly, it is put into dryer and is dried, the use of power is 75W while drying, frequency
Processing is modified for 45 hertz of ultrasonic waves, you can obtains powdery disease-resistant auxiliary material.
Further illustrate, the common adenostema herb extract is prepared by following methods:Common adenostema herb drying and crushing is crossed into screening
The common adenostema herb powder that particle diameter is 2 microns is taken, the ethanol solution that the mass fraction for adding 8 times is 59% is placed in conical flask, is used
Bath temperature carries out water-bath extraction under the conditions of being 39 DEG C, water-bath extract during the use of power is 145W, frequency be 75 hertz of ultrasounds
Ripple assisted extraction, water-bath extraction time are 52 minutes, are centrifuged while hot, the condition of centrifugation is 2750r/min, centrifugation
Time is 40s, takes filtrate to be then concentrated under reduced pressure.In obtained material the content containing organic acid for 5.36mg/g,
The content of alkane type Diterpenes is 2.67mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the Radix Curcumae extract is prepared by following methods:Picking is taken in the noon on the 15th of August in 2016
The rhizome of 12 points of Radix Curcumae, crosses 85 mesh sieves, weighs 75g, put in boiling flask after dry, pulverize after removal of impurities, add quality
Concentration is 79% ethanol solution 320mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz
Assisted extraction 24 minutes, takes out and stands to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.
In obtained material the content containing zingiberene be 1.26mg/g, flavones content 3.25mg/g.
Further illustrate, the Semen Lini extract is prepared by following methods:After linseed is crushed, add compound
Enzyme hydrolysis 18 minutes, carries out 3650r/min using centrifuge and centrifuges 9 minutes, take supernatant, add Sevag reagents and take off albumen 3
It is secondary, 7 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 77%
Carry out alcohol precipitation at 6 DEG C to stay overnight, then carrying out 3800r/min using centrifuge centrifuges 2 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying;The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3: 1;Obtained thing
Containing linolenic content be 3.15mg/g in matter, the content of Lignanoids compounds be 1.64mg/g.
Further illustrate, the mulberry bark extract is prepared by following methods:After root bark of white mulberry rhizome clean dry,
80 mesh sieves are crushed, 117g powder is weighed and adds in conical flask, add 49% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.32g/mL, and vibration stands 24 minutes after 1 minute, in 44 DEG C of temperature, power
30min is extracted under the ultrasound condition of 58W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Mulberry bark extract is can obtain after mist drying.The content containing flavones is 2.31mg/g, the content of sterol in obtained material
For 1.25mg/g.
Further illustrate, the cogongrass rhizome extractive is prepared by following methods:The rhizoma imperatae of autumn picking is crushed
After take 275g to add in triangular flask, add 2% acetic acid methanol solution of cumulative volume, at 38 DEG C, under microwave power 500W into
The row reaction of 23 minutes, is then centrifuged and takes solution, and cogongrass rhizome extractive is can obtain after spray drying.Obtained thing
Contain Cylindrin 2.64mg/g, arundoin 2.15mg/g in matter.
Further illustrate, the dried rehamnnia root extract is prepared by following methods:After dried rehamnnia root remove seed is smashed to pieces, add
48% ethanol solution of mass fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 3 times, 28 minutes every time, extraction
Temperature be 58 DEG C, be concentrated under reduced pressure into 1.12 times of original volume after the filtrate for repeating extraction is merged can obtain dried rehamnnia root
Extract;The volume ratio of dried rehamnnia root and ethanol solution is 1:15.Contain alkaloid 2.67mg/g, martynoside in obtained material
1.96mg/g。
Experiment one:
The cultural method for the emerald green duck of dragon victory that 1-4 of the embodiment of the present invention is prepared carries out growing state record, and record is such as
Table 1 below.
Control group:Fed using vitamin C.
The dragon victory emerald green duck age of every group selection is 3 week old, and one is divided into 5 groups, every group 200, will be sick imperial with hepatitis
The emerald green duck 5 of victory only puts into every group of mixed breeding respectively, carries out infecting experiment, is observed after 3 days, then the disease dragon victory to the hepatitis fallen ill
Emerald green duck treatment, allows the emerald green duck of disease dragon victory freely to drink
Table 1
Number of elements | Morbidity number of elements | Cure number of elements | Incidence | It is efficient | |
Embodiment 1 | 200 | 12 | 10 | 6.0 | 83.3 |
Embodiment 2 | 200 | 12 | 10 | 6.0 | 83.3 |
Embodiment 3 | 200 | 11 | 10 | 5.5 | 90.9 |
Embodiment 4 | 200 | 10 | 9 | 5.0 | 90.0 |
Control group | 200 | 130 | 45 | 65.0 | 34.6 |
As seen from the above table, there is the ability that can reduce incidence of hepatitis rate using the application feed combinations.
Experiment two:
The cultural method for the emerald green duck of dragon victory that 1-4 of the embodiment of the present invention, comparative example 1-6 are prepared carries out growing state note
Record, record such as table 1 below.
Comparative example 1:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:It is strongly fragrant
Golden extract.
Comparative example 2:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:It is sub-
Pockmarks extract.
Comparative example 3:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:Mulberry
White bark extract.
Comparative example 4:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:Wind
Gas grass extract.
Comparative example 5:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:In vain
MAOGEN extract.
Comparative example 6:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:It is dry
Glutinous rehmannia extract.
Comparative example 7:Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is:It is strongly fragrant
Gold, linseed, the root bark of white mulberry, common adenostema herb, rhizoma imperatae, dried rehamnnia root Chinese medicine filter to take filtrate decompression after directly decocting and are dried to powder i.e.
Can.
Control group:Fed using vitamin C.
Dragon victory emerald green duck age per group selection is 3 week old, and by the emerald green duck 5 of disease dragon victory with hepatitis, only input is raised together respectively,
Carry out infecting experiment, observed after 3 days and select 2400 diseased emerald green ducks of dragon victory, be grouped into 12 groups every group 200 at random, each group is distinguished
Using dragon win emerald green duck feed respectively embodiment 1-4, comparative example 1-6, control group food fed, the record record death rate and
Weight contrasts, and continues feeding after 10 days, and the time for comparing recovery normal type, (each group is differed with control group to be calculated back less than 10g
The multiple normal value to control group).Gathered data is listed as follows
Table 2
As seen from the above table, there is the ability that can reduce incidence of hepatitis rate using the application feed combinations, compared to more single
The combination disease-resistant auxiliary material of disease-resistant auxiliary material and the application, effect cannot reach, and reduction incidence in comparison with the present application, dead
Rate, which can reduce, reaches 3-5 times;The disease-resistant auxiliary material being prepared using the application method that compares is compared with Example 7 compared with treating
Required time is short, recovers that the time of weight is fast, and the death rate is low.
Experiment three:Toxicological test:
Acute oral (LD50) > 500mg/kg,
Acute percutaneous (LD50) > 2000mg/kg,
Belong to low toxicity.
A sick duck is taken not do any treatment, as virus control;
Above-described embodiment 1-4, comparative example 1-7 groups, every group is randomly selected one on the 4th day in treatment, is consolidated after weighing weight
Fixed, dissection, takes liver to calculate liver index value, calculates liver index inhibiting rate.
Liver index=liver weight (g)/weight (g) × 100
Liver index inhibiting rate=(virus control group liver index average-experimental group liver index average)/virus control group liver refers to
Number average × 100%
Liver index is bigger, represents that liver pathological changes degree is serious,
Hepatic tissue is fixed with 10% formaldehyde, and ethanol series dehydration, dimethylbenzene is transparent, paraffin embedding, and slice thickness 4-5 is micro-
Rice, HE dyeing, is changed, with ordinary optical microscope observation blood lipid with the liver of Normal group as a comparison.
The influence (n=10) of 3 liver of table verification (virus observation)
As seen from the above table, there is fine inhibition to virus using the feed of the application, embodiment 1-4 compares contrast
Example 1-6 has significant difference, and inhibiting rate can improve at least ten percentage points, and the directly decoction of the comparative example that compares 7 is assigned to disease-resistant
Auxiliary material,
Embodiment 1-4 has the effect for preferably suppressing virus.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, equal change or the modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (8)
1. a kind of cultural method of the emerald green duck of dragon victory, it is characterised in that operate in accordance with the following steps:
For the duckling in 3-6 week old, the feed configured according to the raw material of following mass percentage is fed, 3 times/
My god, and 80-200g/ every time, and by its free choice feeding to all you can eat;
The composition of the feed is as follows:Corn protein powder 50-60%, coconut dregs of rice 10-15%, mung bean flour 8-15%, corn alcohol
Poor 5-8%, steam fish meal 2-5%, salt 1-3%, calcium monohydrogen phosphate 3-5%, disease-resistant auxiliary material 2-5%;
The disease-resistant auxiliary material is made according to percent by weight of following raw material:25%-35% Radix Curcumae extracts, 15%-25%
Semen Lini extract, 15%-20% mulberry bark extracts, 15%-20% common adenostema herbs extract, the extraction of 5%-15% rhizoma imperataes
Thing, 5%-10% dried rehamnnia root extracts;
The preparation method of the disease-resistant auxiliary material is:Above-mentioned extract is mixed according to percent by weight, adds gross weight afterwards
10-30% water be mixed into dope after, add sweetener, hydroxymethyl cellulose, dope:Sweetener:Hydroxymethyl cellulose
Weight ratio is 10:1-2:2-3, after being followed by stirring for uniformly, is put into dryer and is dried, and the use of power is 50- while drying
80W, frequency are modified processing for 30-50 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
2. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the common adenostema herb extract is by following
Method is prepared:Common adenostema herb drying and crushing is sieved the common adenostema herb powder for choosing that particle diameter is 1-2 microns, adds 5-10 times of matter
Measure the ethanol solution that fraction is 50-60% to place in conical flask, carrying out water-bath under the conditions of the use of bath temperature being 30-40 DEG C carries
Take, water-bath extract during the use of power is 100-150W, frequency be 40-80 hertz of ultrasonic wave assisted extractions, water-bath extraction time
For 40-55 minutes, it is centrifuged while hot, the condition of centrifugation is 2500-3000r/min, and the time of centrifugation is 30-50s, is taken
Filtrate is then concentrated under reduced pressure.
3. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Radix Curcumae extract is by with lower section
Method is prepared:Picking is taken in the rhizome of the Radix Curcumae of the 12 noon on the 15th of August in 2016, mistake after dry, pulverize after removal of impurities
50-100 mesh sieves, weigh 50-80g, put in boiling flask, add the ethanol solution 250-350mL that mass concentration is 75-80%,
Carry out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz of assisted extractions 20-25 minute, take out standing
To room temperature, 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume are filtered to take.
4. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Semen Lini extract is by following
Method is prepared:After linseed is crushed, complex enzyme for hydrolyzing is added 10-20 minutes, 3500-4000r/ is carried out using centrifuge
Min is centrifuged 5-10 minutes, takes supernatant, is added Sevag reagents and is taken off albumen 2-3 times, each 5-8 minutes, and it is dense then to carry out vacuum
After being reduced to 1.12 times of original volume, addition mass fraction carries out alcohol precipitation for the ethanol solution of 70-80% at 5-8 DEG C and stays overnight, and connects
And carry out 3000-4000r/min centrifugations 1-3 minutes using centrifuge, precipitation is taken out after standing, is extracted after steam drying
Thing;The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3-4: 1.
5. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the mulberry bark extract is by following
Method is prepared:After root bark of white mulberry rhizome clean dry, 50-100 mesh sieves are crushed, 100-120g powder is weighed and adds taper
In bottle, the volume fraction 40-50% ethanol solutions of 500mL are added, the mass concentration for being subsequently added into 10mL is 0.3-0.4g/mL's
Ammonium sulfate, vibration 1 minute after stand 20-25 minutes, 40-50 DEG C of temperature, power 50-60W ultrasound condition under extract
30min, filters, and solution is moved into stratification in separatory funnel.Upper solution is taken, Sang Bai is can obtain after being spray-dried
Bark extract.
6. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the cogongrass rhizome extractive is by following
Method is prepared:Take 200-300g to add in triangular flask after the rhizoma imperatae of autumn picking is crushed, add the 1-3% of cumulative volume
Acetic acid methanol solution, the reaction of 20-25 minutes is carried out at 35-40 DEG C, under microwave power 500W, is then centrifuged
Solution is taken, cogongrass rhizome extractive is can obtain after spray drying.
7. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the dried rehamnnia root extract is by following
Method is prepared:After dried rehamnnia root remove seed is smashed to pieces, add mass fraction 40-50% ethanol solutions and carry out refluxing extraction, reflux
The condition of extraction is to repeat extraction 2-3 times, 20-30 minutes each, and the temperature of extraction is 50-60 DEG C, will repeat the filtrate of extraction
Carry out being concentrated under reduced pressure into original volume after merging 1.12 times can obtain dried rehamnnia root extract;Dried rehamnnia root and the volume of ethanol solution
Than for 1:10-25.
8. the cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the sweetener is xylitol or low
Polyisomaltose.
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CN103355504A (en) * | 2013-08-05 | 2013-10-23 | 山东新希望六和集团有限公司 | Formula feed for 0-7 weeks-old broiler chickens and preparation method thereof |
CN103719554A (en) * | 2013-12-30 | 2014-04-16 | 何贻峰 | Meat duck feed |
CN106172216A (en) * | 2016-07-26 | 2016-12-07 | 广西陆川县润润养殖有限公司 | A kind of duck culturing method preventing and treating duck viral hepatitis |
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CN103355504A (en) * | 2013-08-05 | 2013-10-23 | 山东新希望六和集团有限公司 | Formula feed for 0-7 weeks-old broiler chickens and preparation method thereof |
CN103719554A (en) * | 2013-12-30 | 2014-04-16 | 何贻峰 | Meat duck feed |
CN106172216A (en) * | 2016-07-26 | 2016-12-07 | 广西陆川县润润养殖有限公司 | A kind of duck culturing method preventing and treating duck viral hepatitis |
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