CN107912363A - A kind of disease-resistant cultural method of the emerald green duck of dragon victory - Google Patents
A kind of disease-resistant cultural method of the emerald green duck of dragon victory Download PDFInfo
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- CN107912363A CN107912363A CN201711191627.XA CN201711191627A CN107912363A CN 107912363 A CN107912363 A CN 107912363A CN 201711191627 A CN201711191627 A CN 201711191627A CN 107912363 A CN107912363 A CN 107912363A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to cultural technique field, more particularly to a kind of disease-resistant cultural method of the emerald green duck of dragon victory, operate in accordance with the following steps：For the duckling in 36 week old, the feed configured according to the raw material of following mass percentage is fed, 3 times/day, and 80 200g/ every time, and by its free choice feeding to all you can eat；The composition of the feed is as follows：Corn flour 50 60%, dregs of beans 10 15%, wheat bran 8 15%, premix 5 8%, rape cake 2 5%, salt 1 3%, calcium monohydrogen phosphate 3 5%, disease-resistant auxiliary material 2 5%.Advantage of the present invention：The feed for finding to feed the application in periodic time by substantial amounts of research can not only improve growth rate, also have the effect for suppressing virus well, so that the survival rate of the emerald green duck of dragon victory is improved, and then the purpose increased income.
Description
Technical field
The present invention relates to cultural technique field, more particularly to a kind of disease-resistant cultural method of the emerald green duck of dragon victory.
Background technology
Dragon wins emerald green duck, and the emerald green duck of dragon victory is blackish green because its whole body feather black carries, and gains the name in the gloss as emerald.
Main product is also named " foreign hole duck ", means the duck that people from Miao ethnic group supports in Miao ethnic group compatriot residence, locality.It is mainly distributed on
Guangxi Zhuang Autonomous Region Long Shengxian Ma Di, big Jiang Xiang.According to records, " foreign hole duck " feather black bands bird with red feathers, produces green-shell egg, gregariousness
It is good, it is suitable for the water surface and rice field is put in a suitable place to breed.The emerald green duck of dragon victory has unique shape and appearance, has high ornamental value, and meat is thin
Tender no fishy smell, is the rare genetic resources of duck varieties.
The content of the invention
The goal of the invention of the present invention is：A kind of disease-resistant cultural method of the emerald green duck of dragon victory is provided, is sent out by substantial amounts of research
The feed that present periodic time feeds the application can not only improve growth rate, also have the effect for suppressing virus well, from
And the survival rate of the emerald green duck of dragon victory is improved, and then the purpose increased income.
In order to realize foregoing invention purpose, the technical solution adopted by the present invention is as follows：
A kind of disease-resistant cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps：
For the duckling in 3-6 week old, the feed configured according to the raw material of following mass percentage is fed,
3 times/day, and 80-200g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 50-60%, dregs of beans 10-15%, wheat bran 8-15%, premix 5-8%,
Rape cake 2-5%, salt 1-3%, calcium monohydrogen phosphate 3-5%, disease-resistant auxiliary material 2-5%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：25%-35% capillary extracts, 15%-
25% Endothelium Corneum Gigeriae Galli extract, 15%-20% Herba Plantaginis extracts, 15%-20% Spora Lygodii extracts, 5%-15% barberries carry
Take thing, 5%-10% Stenoloma chusana extracts；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After the 10-30% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：It is viscous
Thick thing is 0.1-0.3 according to ratio of weight and number:0.5-0.7:After 12 stir evenly, be put into dryer and dried, drying it is same
When the use of power is 50-80W, frequency be that 30-50 hertz of ultrasonic waves are modified processing, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the Spora Lygodii extract is prepared by following methods：It is 1-2 microns that particle diameter is chosen in sieving
Lygodium japonicum, the ethanol solution that the mass fraction for adding 5-10 times is 50-60% are placed in conical flask, the use of bath temperature are 30-
Carry out water-bath extraction under the conditions of 40 DEG C, water-bath extract during the use of power is 100-150W, frequency be that 40-80 hertz of ultrasonic waves are auxiliary
Extraction is helped, water-bath extraction time is 40-55 minutes, is centrifuged while hot, and the condition of centrifugation is 2500-3000r/min, from
The time of the heart is 30-50s, takes filtrate to be then concentrated under reduced pressure.It is containing linoleic content in obtained material
1.26mg/g-2.05mg/g, stearic content are 1.02mg/g-3.26mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the capillary extract is prepared by following methods：Picking is taken in 10 5 points of morning of September
Oriental wormwood herb, crosses 50-100 mesh sieves, weighs 50-80g, put in boiling flask after dry, pulverize after removal of impurities, it is dense to add quality
Spend the ethanol solution 250-350mL for 75-80%, carry out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency 40
Hertz assisted extraction 20-25 minutes, takes out and stands to room temperature, filter to take filtrate and then carry out being concentrated under reduced pressure into the 1.01 of original volume
Times.In obtained material the content containing cumarin be 0.98mg/g-1.31mg/g, flavones content 1.15mg/g-
8.02mg/g。
Further illustrate, the Endothelium Corneum Gigeriae Galli extract is prepared by following methods：After the membrane of a chicken's gizzard is crushed, add compound
Enzyme hydrolysis 10-20 minutes, carries out 3500-4000r/min using centrifuge and centrifuges 5-10 minutes, take supernatant, add Sevag examinations
Agent takes off albumen 2-3 times, each 5-8 minutes, then carries out after being concentrated in vacuo to 1.12 times of original volume, adds mass fraction and is
The ethanol solution of 70-80% carries out alcohol precipitation at 5-8 DEG C and stays overnight, and then carries out 3000-4000r/min centrifugations 1- using centrifuge
3 minutes, precipitation is taken out after standing, extract is obtained after steam drying；The Sevag reagents be according to volume ratio chloroform-
N-butanol 3-4: 1 is mixed to get；Content containing choline is 9.59mg/g-12.06mg/g in obtained material, gastric hormone contains
Measure as 9.59mg/g-12.06mg/g.
Further illustrate, the Herba Plantaginis extract is prepared by following methods：After Asiatic plantain rhizome clean dry,
50-100 mesh sieves are crushed, 100-120g powder is weighed and adds in conical flask, add the volume fraction 40-50% ethanol of 500mL
Solution, the mass concentration for being subsequently added into 10mL are the ammonium sulfate of 0.3-0.4g/m L, and vibration stands 20-25 points after 1 minute
Clock, 40-50 DEG C of temperature, power 50-60W ultrasound condition under extract 30min, filter, will solution move into separatory funnel in it is quiet
Put layering.Upper solution is taken, Herba Plantaginis extract is can obtain after being spray-dried.Containing containing flavones in obtained material
The content measured as 1.63mg/g-3.28mg/g, triterpene is 1.54mg/g-4.10mg/g, the content of sterol is 1.63mg/g-
4.12mg/g。
Further illustrate, the Radix Berberidis extract is prepared by following methods：The root skin for harvesting berberis brachypoda Maxim is obtained
To barberry rhizome crush after take 200-300g to add in triangular flask, add the acetic acid methanol solution of the 1-3% of cumulative volume,
The reaction of 20-25 minutes is carried out at 35-40 DEG C, under microwave power 500W, is then centrifuged and takes solution, after spray drying
It can obtain Radix Berberidis extract.Contain jamaicin 1.63mg/g-3.65mg/g, jateorrhizine 1.06mg/g- in obtained material
5.01mg/g。
Further illustrate, the Stenoloma chusana extract is prepared by following methods：After Stenoloma chusana drying and crushing, quality is added
Fraction 40-50% ethanol solutions carry out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2-3 times, 20-30 minutes each,
The temperature of extraction is 50-60 DEG C, and be concentrated under reduced pressure into original volume after the filtrate for repeating extraction is merged 1.12 times can obtain
To Stenoloma chusana extract；The volume ratio of Stenoloma chusana and ethanol solution is 1:10-25.Contain cupreol 1.62mg/ in obtained material
G-4.32mg/g, glucosides 1.58mg/g-4.30mg/g.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Corn flour of the present invention, dregs of beans, wheat bran, premix, rape cake, salt, calcium monohydrogen phosphate, disease-resistant auxiliary material are after proportioning
Reach and contained organic matter 45.23-64.26%, trace element 10.26-15.36%, effective carbon 21.36-34.26%, effective
Calcium 18.62-35.28% can meet the emerald green duck nutrient elements required for the growth of dragon victory, promote growth, and with the addition of disease-resistant auxiliary material
There is good anti-hepatitis, improve the survival rate of the emerald green duck of dragon victory.
The effect of Chinese medicine active compound：Oriental wormwood cold nature, it is bitter, pungent；Converge to the spleen channel, stomach, bladder warp；Clearing away damp-heat, removing jaundice subcutaneous ulcer.Sea
Jinsha is sweet in flavor, cold in nature；Return bladder warp, small intestinl channel；Eliminating dampness and heat, treating stranguria analgesic.The membrane of a chicken's gizzard is mild-natured, sweet in flavor.Converge to the spleen channel, stomach,
Small intestinl channel, bladder warp.Stomach strengthening and digestion promoting, arresting seminal emission.Asiatic plantain is cold in nature, sweet in flavor.Return liver warp, kidney channel, lung channel, small intestinl channel；Heat-clearing
Diuresis, eliminating the phlegm, cool blood, removing toxic substances.Barberry is bitter, trembles with fear；Return liver, stomach, large intestine channel；Heat-clearing, dampness removing, dissipates the stasis of blood.Stenoloma chusana slight bitter, trembles with fear；Clearly
Fire, brings down a fever, dampness removing, hemostasis.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.This specification (including any accessory claim, summary)
Disclosed in any feature, unless specifically stated, can be replaced by other alternative features that are equivalent or have similar purpose.
I.e., unless specifically stated, each feature is an example in a series of equivalent or similar characteristics.
Embodiment 1
A kind of disease-resistant cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps：
For the duckling in 3 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 80g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 60%, dregs of beans 15%, wheat bran 12%, premix 5%, rape cake 2%,
Salt 1%, calcium monohydrogen phosphate 3%, disease-resistant auxiliary material 2%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：35% capillary extract, 25% the membrane of a chicken's gizzard
Extract, 15% Herba Plantaginis extract, 15% Spora Lygodii extract, 5% Radix Berberidis extract, 5% Stenoloma chusana extract；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 10% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：Dope
It is 0.1 according to ratio of weight and number:0.6:After 12 stir evenly, it is put into dryer and is dried, is using power while drying
50W, frequency are modified processing for 30 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the Spora Lygodii extract is prepared by following methods：The sea that particle diameter is 1 micron is chosen in sieving
Jinsha, add 5 times mass fraction be 50% ethanol solution place conical flask in, using bath temperature be 30 DEG C under the conditions of into
Row water-bath is extracted, water-bath extract during the use of power is 100W, frequency be 40 hertz of ultrasonic wave assisted extractions, water-bath extraction time
For 40 minutes, be centrifuged while hot, the condition of centrifugation is 2500r/min, and the time of centrifugation is 30s, take filtrate then into
Row is concentrated under reduced pressure.Containing linoleic content be 1.26mg/g in obtained material, stearic content be 1.02mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the capillary extract is prepared by following methods：Picking is taken in 10 5 points of morning of September
Oriental wormwood herb, crosses 50 mesh sieves, weighs 50g, put in boiling flask after dry, pulverize after removal of impurities, add mass concentration and be
75% ethanol solution 250mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be that 40 hertz of auxiliary carry
Take 20 minutes, take out and stand to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.Obtain
In material the content containing cumarin be 0.98mg/g, flavones content 1.15mg/g.
Further illustrate, the Endothelium Corneum Gigeriae Galli extract is prepared by following methods：After the membrane of a chicken's gizzard is crushed, add compound
Enzyme hydrolysis 10 minutes, carries out 3500r/min using centrifuge and centrifuges 5 minutes, take supernatant, add Sevag reagents and take off albumen 2
It is secondary, 5 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 70%
Carry out alcohol precipitation at 5 DEG C to stay overnight, then carrying out 3000r/min using centrifuge centrifuges 1 minute, and precipitation, steam are taken out after standing
Extract is obtained after drying；The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3: 1；Obtained thing
In matter the content containing choline be 9.59mg/g, the content of gastric hormone be 9.59mg/g.
Further illustrate, the Herba Plantaginis extract is prepared by following methods：After Asiatic plantain rhizome clean dry,
50 mesh sieves are crushed, 100g powder is weighed and adds in conical flask, add 40% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.3g/m L, and vibration stands 20 minutes after 1 minute, in 40 DEG C of temperature, power
30min is extracted under the ultrasound condition of 50W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Herba Plantaginis extract is can obtain after mist drying.The content containing flavones is 1.63mg/g, the content of triterpene in obtained material
Content for 1.54mg/g, sterol is 1.63mg/g.
Further illustrate, the Radix Berberidis extract is prepared by following methods：The root skin for harvesting berberis brachypoda Maxim is obtained
To barberry rhizome crush after take 200g to add in triangular flask, 1% acetic acid methanol solution of cumulative volume is added, at 35 DEG C
Under, the reaction of 20 minutes is carried out under microwave power 500W, be then centrifuged and take solution, i.e. available three after spray drying
Pin extract.Contain jamaicin 1.63mg/g, jateorrhizine 1.06mg/g in obtained material.
Further illustrate, the Stenoloma chusana extract is prepared by following methods：After Stenoloma chusana drying and crushing, quality is added
40% ethanol solution of fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2 times, 20 minutes every time, the temperature of extraction
Spend for 50 DEG C, carry out being concentrated under reduced pressure into original volume after the filtrate for repeating extraction is merged 1.12 times can obtain Stenoloma chusana extraction
Thing；The volume ratio of Stenoloma chusana and ethanol solution is 1:20.Contain cupreol 1.62mg/g, glucosides 1.58mg/ in obtained material
g。
Embodiment 2
A kind of disease-resistant cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps：
For the duckling in 5 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 100g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 59%, dregs of beans 15%, wheat bran 8%, premix 6%, rape cake 3%, food
Salt 2%, calcium monohydrogen phosphate 4%, disease-resistant auxiliary material 3%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：25% capillary extract, 15% the membrane of a chicken's gizzard
Extract, 15% Herba Plantaginis extract, 20% Spora Lygodii extract, 15% Radix Berberidis extract, 10% Stenoloma chusana extract；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 30% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：Dope
It is 0.3 according to ratio of weight and number:0.5:After 12 stir evenly, it is put into dryer and is dried, is using power while drying
80W, frequency are modified processing for 50 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the Spora Lygodii extract is prepared by following methods：The sea that particle diameter is 2 microns is chosen in sieving
Jinsha, the ethanol solution that the mass fraction for adding 10 times is 60% are placed in conical flask, under the conditions of the use of bath temperature being 40 DEG C
Carry out water-bath extraction, water-bath extract during the use of power is 150W, frequency be 80 hertz of ultrasonic wave assisted extractions, when water-bath is extracted
Between be 55 minutes, be centrifuged while hot, the condition of centrifugation is 3000r/min, and the time of centrifugation is 50s, takes filtrate then
It is concentrated under reduced pressure.Containing linoleic content be 1.56mg/g in obtained material, stearic content be 2.12mg/
g。
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the capillary extract is prepared by following methods：Picking is taken in 10 5 points of morning of September
Oriental wormwood herb, crosses 100 mesh sieves, weighs 80g, put in boiling flask after dry, pulverize after removal of impurities, add mass concentration and be
80% ethanol solution 350mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be that 40 hertz of auxiliary carry
Take 25 minutes, take out and stand to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.Obtain
In material the content containing cumarin be 1.03mg/g, flavones content 2.64mg/g.
Further illustrate, the Endothelium Corneum Gigeriae Galli extract is prepared by following methods：After the membrane of a chicken's gizzard is crushed, add compound
Enzyme hydrolysis 20 minutes, carries out 4000r/min using centrifuge and centrifuges 10 minutes, take supernatant, add Sevag reagents and take off albumen 3
It is secondary, 8 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 80%
Carry out alcohol precipitation at 8 DEG C to stay overnight, then carrying out 4000r/min using centrifuge centrifuges 3 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying；The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 4: 1；Obtained thing
In matter the content containing choline be 10.25mg/g, the content of gastric hormone be 10.34mg/g.
Further illustrate, the Herba Plantaginis extract is prepared by following methods：After Asiatic plantain rhizome clean dry,
100 mesh sieves are crushed, 120g powder is weighed and adds in conical flask, add 50% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.4g/m L, and vibration stands 25 minutes after 1 minute, in temperature 50 C, power
30min is extracted under the ultrasound condition of 60W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Herba Plantaginis extract is can obtain after mist drying.The content containing flavones is 2.16mg/g, the content of triterpene in obtained material
Content for 2.07mg/g, sterol is 2.03mg/g.
Further illustrate, the Radix Berberidis extract is prepared by following methods：The root skin for harvesting berberis brachypoda Maxim is obtained
To barberry rhizome crush after take 300g to add in triangular flask, 3% acetic acid methanol solution of cumulative volume is added, at 40 DEG C
Under, the reaction of 25 minutes is carried out under microwave power 500W, be then centrifuged and take solution, i.e. available three after spray drying
Pin extract.Contain jamaicin 2.14mg/g, jateorrhizine 2.38mg/g in obtained material.
Further illustrate, the Stenoloma chusana extract is prepared by following methods：After Stenoloma chusana drying and crushing, quality is added
50% ethanol solution of fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 3 times, 30 minutes every time, the temperature of extraction
Spend for 60 DEG C, carry out being concentrated under reduced pressure into original volume after the filtrate for repeating extraction is merged 1.12 times can obtain Stenoloma chusana extraction
Thing；The volume ratio of Stenoloma chusana and ethanol solution is 1:25.Contain cupreol 2.56mg/g, glucosides 3.20mg/ in obtained material
g。
Embodiment 3
A kind of disease-resistant cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps：
For the duckling in 6 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 120g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 52%, dregs of beans 12%, wheat bran 15%, premix 7%, rape cake 4%,
Salt 2%, calcium monohydrogen phosphate 4%, disease-resistant auxiliary material 4%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：26% capillary extract, 16% the membrane of a chicken's gizzard
Extract, 20% Herba Plantaginis extract, 18% Spora Lygodii extract, 12% Radix Berberidis extract, 8% Stenoloma chusana extract；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 20% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：Dope
It is 0.1 according to ratio of weight and number:0.7:After 12 stir evenly, it is put into dryer and is dried, is using power while drying
60W, frequency are modified processing for 35 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the Spora Lygodii extract is prepared by following methods：It is 1.5 microns that particle diameter is chosen in sieving
Lygodium japonicum, the ethanol solution that the mass fraction for adding 7 times is 53% are placed in conical flask, under the conditions of the use of bath temperature being 34 DEG C
Carry out water-bath extraction, water-bath extract during the use of power is 125W, frequency be 50 hertz of ultrasonic wave assisted extractions, when water-bath is extracted
Between be 45 minutes, be centrifuged while hot, the condition of centrifugation be 2650r/min, and the time of centrifugation is 35, take filtrate then into
Row is concentrated under reduced pressure.Containing linoleic content be 1.57mg/g in obtained material, stearic content be 2.11mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the capillary extract is prepared by following methods：Picking is taken in 10 5 points of morning of September
Oriental wormwood herb, crosses 60 mesh sieves, weighs 60g, put in boiling flask after dry, pulverize after removal of impurities, add mass concentration and be
76% ethanol solution 300mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be that 40 hertz of auxiliary carry
Take 23 minutes, take out and stand to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.Obtain
In material the content containing cumarin be 1.04mg/g, flavones content 2.43mg/g.
Further illustrate, the Endothelium Corneum Gigeriae Galli extract is prepared by following methods：After the membrane of a chicken's gizzard is crushed, add compound
Enzyme hydrolysis 18 minutes, carries out 3600r/min using centrifuge and centrifuges 7 minutes, take supernatant, add Sevag reagents and take off albumen 2
It is secondary, 7 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 72%
Carry out alcohol precipitation at 6 DEG C to stay overnight, then carrying out 3800r/min using centrifuge centrifuges 2 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying；The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 4: 1；Obtained thing
In matter the content containing choline be 10.25mg/g, the content of gastric hormone be 10.86mg/g.
Further illustrate, the Herba Plantaginis extract is prepared by following methods：After Asiatic plantain rhizome clean dry,
70 mesh sieves are crushed, 110g powder is weighed and adds in conical flask, add 43% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.35g/m L, and vibration stands 23 minutes after 1 minute, in 47 DEG C of temperature, power
30min is extracted under the ultrasound condition of 53W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Herba Plantaginis extract is can obtain after mist drying.The content containing flavones is 2.41mg/g, the content of triterpene in obtained material
Content for 3.17mg/g, sterol is 3.17mg/g.
Further illustrate, the Radix Berberidis extract is prepared by following methods：The root skin for harvesting berberis brachypoda Maxim is obtained
To barberry rhizome crush after take 250g to add in triangular flask, 2% acetic acid methanol solution of cumulative volume is added, at 38 DEG C
Under, the reaction of 22 minutes is carried out under microwave power 500W, be then centrifuged and take solution, i.e. available three after spray drying
Pin extract.Contain jamaicin 2.76mg/g, jateorrhizine 3.46mg/g in obtained material.
Further illustrate, the Stenoloma chusana extract is prepared by following methods：After Stenoloma chusana drying and crushing, quality is added
43% ethanol solution of fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 2 times, 28 minutes every time, the temperature of extraction
Spend for 55 DEG C, carry out being concentrated under reduced pressure into original volume after the filtrate for repeating extraction is merged 1.12 times can obtain Stenoloma chusana extraction
Thing；The volume ratio of Stenoloma chusana and ethanol solution is 1:10.Contain cupreol 3.14mg/g, glucosides 3.07mg/ in obtained material
g。
Embodiment 4
A kind of disease-resistant cultural method of the emerald green duck of dragon victory, operates in accordance with the following steps：
For the duckling in 4 week old, the feed configured according to the raw material of following mass percentage is fed, and 3
Times/day, and 180g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 50%, dregs of beans 10%, wheat bran 14%, premix 8%, rape cake 5%,
Salt 3%, calcium monohydrogen phosphate 5%, disease-resistant auxiliary material 5%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：30% capillary extract, 20% the membrane of a chicken's gizzard
Extract, 16% Herba Plantaginis extract, 17% Spora Lygodii extract, 10% Radix Berberidis extract, 7% Stenoloma chusana extract；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, it is rear to add always
After 25% water of weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：Dope
It is 0.2 according to ratio of weight and number:0.6:After 12 stir evenly, it is put into dryer and is dried, is using power while drying
75W, frequency are modified processing for 45 hertz of ultrasonic waves, you can obtain powdery disease-resistant auxiliary material.
Further illustrate, the Spora Lygodii extract is prepared by following methods：The sea that particle diameter is 2 microns is chosen in sieving
Jinsha, add 8 times mass fraction be 59% ethanol solution place conical flask in, using bath temperature be 39 DEG C under the conditions of into
Row water-bath is extracted, water-bath extract during the use of power is 145W, frequency be 75 hertz of ultrasonic wave assisted extractions, water-bath extraction time
For 52 minutes, be centrifuged while hot, the condition of centrifugation is 2750r/min, and the time of centrifugation is 40s, take filtrate then into
Row is concentrated under reduced pressure.Containing linoleic content be 2.05mg/g in obtained material, stearic content be 3.26mg/g.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
Further illustrate, the capillary extract is prepared by following methods：Picking is taken in 10 5 points of morning of September
Oriental wormwood herb, crosses 85 mesh sieves, weighs 75g, put in boiling flask after dry, pulverize after removal of impurities, add mass concentration and be
79% ethanol solution 320mL, carries out alcohol extracting, also using ultrasonic power be 100W in alcohol extracting, frequency be that 40 hertz of auxiliary carry
Take 24 minutes, take out and stand to room temperature, filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.Obtain
In material the content containing cumarin be 1.31mg/g, flavones content 8.02mg/g.
Further illustrate, the Endothelium Corneum Gigeriae Galli extract is prepared by following methods：After the membrane of a chicken's gizzard is crushed, add compound
Enzyme hydrolysis 18 minutes, carries out 3650r/min using centrifuge and centrifuges 9 minutes, take supernatant, add Sevag reagents and take off albumen 3
It is secondary, 7 minutes every time, then carry out after being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 77%
Carry out alcohol precipitation at 6 DEG C to stay overnight, then carrying out 3800r/min using centrifuge centrifuges 2 minutes, and precipitation, steam are taken out after standing
Extract is obtained after drying；The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3: 1；Obtained thing
In matter the content containing choline be 12.06mg/g, the content of gastric hormone be 12.06mg/g.
Further illustrate, the Herba Plantaginis extract is prepared by following methods：After Asiatic plantain rhizome clean dry,
80 mesh sieves are crushed, 117g powder is weighed and adds in conical flask, add 49% ethanol solution of volume fraction of 500mL, then add
The mass concentration for entering 10mL is the ammonium sulfate of 0.32g/m L, and vibration stands 24 minutes after 1 minute, in 44 DEG C of temperature, power
30min is extracted under the ultrasound condition of 58W, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, is sprayed
Herba Plantaginis extract is can obtain after mist drying.The content containing flavones is 3.28mg/g, the content of triterpene in obtained material
Content for 4.10mg/g, sterol is 4.12mg/g.
Further illustrate, the Radix Berberidis extract is prepared by following methods：The root skin for harvesting berberis brachypoda Maxim is obtained
To barberry rhizome crush after take 275g to add in triangular flask, 2% acetic acid methanol solution of cumulative volume is added, at 38 DEG C
Under, the reaction of 23 minutes is carried out under microwave power 500W, be then centrifuged and take solution, i.e. available three after spray drying
Pin extract.Contain jamaicin 3.65mg/g, jateorrhizine 5.01mg/g in obtained material.
Further illustrate, the Stenoloma chusana extract is prepared by following methods：After Stenoloma chusana drying and crushing, quality is added
48% ethanol solution of fraction carries out refluxing extraction, and the condition of refluxing extraction is to repeat extraction 3 times, 28 minutes every time, the temperature of extraction
Spend for 58 DEG C, carry out being concentrated under reduced pressure into original volume after the filtrate for repeating extraction is merged 1.12 times can obtain Stenoloma chusana extraction
Thing；The volume ratio of Stenoloma chusana and ethanol solution is 1:15.Contain cupreol 4.32mg/g, glucosides 4.30mg/ in obtained material
g。
Experiment one：
The disease-resistant cultural method for the emerald green duck of dragon victory that 1-4 of the embodiment of the present invention is prepared carries out growing state record, note
Record such as table 1 below.
Control group：Fed using vitamin C.
The dragon victory emerald green duck age of every group selection is 3 week old, and one is divided into 5 groups, every group 200, will be sick imperial with hepatitis
The emerald green duck 5 of victory only puts into every group of mixed breeding respectively, carries out infecting experiment, is observed after 3 days, then the disease dragon victory to the hepatitis fallen ill
Emerald green duck treatment, allows the emerald green duck of disease dragon victory freely to drink
Table 1
| Number of elements | Morbidity number of elements | Cure number of elements | Incidence | It is efficient | |
| Embodiment 1 | 200 | 14 | 12 | 7.0 | 85.7 |
| Embodiment 2 | 200 | 12 | 10 | 6.0 | 83.3 |
| Embodiment 3 | 200 | 12 | 11 | 6.0 | 91.6 |
| Embodiment 4 | 200 | 10 | 9 | 5.0 | 90.0 |
| Control group | 200 | 138 | 41 | 69.0 | 29.7 |
As seen from the above table, there is the ability that can reduce incidence of hepatitis rate using the application feed combinations.
Experiment two：
The disease-resistant cultural method for the emerald green duck of dragon victory that 1-4 of the embodiment of the present invention, comparative example 1-6 are prepared carries out growth feelings
Condition records, record such as table 1 below.
Comparative example 1：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Mattress
Old extract.
Comparative example 2：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Chicken
Interior gold extract.
Comparative example 3：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Car
Preceding grass extract.
Comparative example 4：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Sea
Jinsha extract.
Comparative example 5：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Three
Pin extract.
Comparative example 6：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Crow
Fragrant-flowered garlic extract.
Comparative example 7：Feeding patterns is identical, and feed is substantially same as Example 3, and difference is that disease-resistant auxiliary material component is：Mattress
The old, the membrane of a chicken's gizzard, Asiatic plantain, Lygodium japonicum, barberry, Stenoloma chusana Chinese medicine filter to take filtrate decompression after directly decocting and are dried to powder.
Control group：Fed using vitamin C.
Dragon victory emerald green duck age per group selection is 3 week old, and by the emerald green duck 5 of disease dragon victory with hepatitis, only input is raised together respectively,
Carry out infecting experiment, observed after 3 days and select 2400 diseased emerald green ducks of dragon victory, be grouped into 12 groups every group 200 at random, each group is distinguished
Using dragon win emerald green duck feed respectively embodiment 1-4, comparative example 1-6, control group food fed, the record record death rate and
Weight contrasts, and continues feeding after 10 days, and the time for comparing recovery normal type, (each group is differed with control group to be calculated back less than 10g
The multiple normal value to control group).Gathered data is listed as follows
Table 2
As seen from the above table, there is the ability that can reduce incidence of hepatitis rate using the application feed combinations, compared to more single
The combination disease-resistant auxiliary material of disease-resistant auxiliary material and the application, effect cannot reach, and reduction incidence in comparison with the present application, dead
Rate, which can reduce, reaches 3-5 times；The disease-resistant auxiliary material being prepared using the application method that compares is compared with Example 7 compared with treating
Required time is short, recovers that the time of weight is fast, and the death rate is low.
Experiment three：Toxicological test：
Acute oral (LD50) ＞ 500mg/kg,
Acute percutaneous (LD50) ＞ 2000mg/kg,
Belong to low toxicity.
A sick duck is taken not do any treatment, as virus control；
Above-described embodiment 1-4, comparative example 1-7 groups, every group is randomly selected one on the 4th day in treatment, is consolidated after weighing weight
Fixed, dissection, takes liver to calculate liver index value, calculates liver index inhibiting rate.
Liver index=liver weight (g)/weight (g) × 100
Liver index inhibiting rate=(virus control group liver index average-experimental group liver index average)/virus control group liver refers to
Number average × 100%
Liver index is bigger, represents that liver pathological changes degree is serious,
Hepatic tissue is fixed with 10% formaldehyde, and ethanol series dehydration, dimethylbenzene is transparent, paraffin embedding, and slice thickness 4-5 is micro-
Rice, HE dyeing, is changed, with ordinary optical microscope observation blood lipid with the liver of Normal group as a comparison.
The influence (n=10) of 3 liver of table verification (virus observation)
As seen from the above table, there is fine inhibition to virus using the feed of the application, embodiment 1-4 compares contrast
Example 1-6 has significant difference, and inhibiting rate can improve at least ten percentage points, and the directly decoction of the comparative example that compares 7 is assigned to disease-resistant
Auxiliary material, embodiment 1-4 have the effect for preferably suppressing virus.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, equal change or the modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (8)
1. a kind of disease-resistant cultural method of the emerald green duck of dragon victory, it is characterised in that operate in accordance with the following steps：
For the duckling in 3-6 week old, the feed configured according to the raw material of following mass percentage is fed, 3 times/
My god, and 80-200g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 50-60%, dregs of beans 10-15%, wheat bran 8-15%, premix 5-8%, vegetable seed
Cake 2-5%, salt 1-3%, calcium monohydrogen phosphate 3-5%, disease-resistant auxiliary material 2-5%；
The disease-resistant auxiliary material is made according to percent by weight of following raw material：25%-35% capillary extracts, 15%-25%
Endothelium Corneum Gigeriae Galli extract, 15%-20% Herba Plantaginis extracts, 15%-20% Spora Lygodii extracts, the extraction of 5%-15% barberries
Thing, 5%-10% Stenoloma chusana extracts；
The preparation method of the disease-resistant auxiliary material is：Above-mentioned extract is mixed according to percent by weight, adds gross weight afterwards
10-30% water be mixed into dope after, add sweetener, hydroxymethyl cellulose, sweetener：Hydroxymethyl cellulose：Dope
It is 0.1-0.3 according to ratio of weight and number:0.5-0.7:After 12 stir evenly, it is put into dryer and is dried, drying while makes
Be 50-80W with power, frequency be that 30-50 hertz of ultrasonic waves are modified processing, you can obtain powdery disease-resistant auxiliary material.
2. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Spora Lygodii extract by
Following methods are prepared：The Lygodium japonicum that particle diameter is 1-2 microns is chosen in sieving, and the mass fraction for adding 5-10 times is 50-60%
Ethanol solution place conical flask in, using bath temperature be 30-40 DEG C under the conditions of carry out water-bath extraction, water-bath extract during make
Be 100-150W with power, frequency be 40-80 hertz of ultrasonic wave assisted extractions, water-bath extraction time is 40-55 minutes, while hot into
Row centrifuge, the condition of centrifugation is 2500-3000r/min, and the time of centrifugation is 30-50s, take filtrate then carry out depressurizing it is dense
Contracting.
3. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the capillary extract by with
Lower section method is prepared：Picking is taken to cross 50-100 after dry, pulverize after removal of impurities in the oriental wormwood herb in 10 5 points of morning of September
Mesh sieve, weighs 50-80g, puts in boiling flask, adds the ethanol solution 250-350mL that mass concentration is 75-80%, carries out alcohol
Carry, also using ultrasonic power be 100W in alcohol extracting, frequency be 40 hertz of assisted extractions 20-25 minutes, take out and stand to room temperature,
Filter to take 1.01 times that filtrate then carries out being concentrated under reduced pressure into original volume.
4. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Endothelium Corneum Gigeriae Galli extract by
Following methods are prepared：After the membrane of a chicken's gizzard is crushed, complex enzyme for hydrolyzing is added 10-20 minutes, 3500- is carried out using centrifuge
4000r/min is centrifuged 5-10 minutes, takes supernatant, is added Sevag reagents and is taken off albumen 2-3 times, each 5-8 minutes, then carries out
After being concentrated in vacuo to 1.12 times of original volume, add the ethanol solution that mass fraction is 70-80% and alcohol precipitation mistake is carried out at 5-8 DEG C
At night, then carry out 3000-4000r/min using centrifuge and centrifuge 1-3 minutes, precipitation is taken out after standing, is obtained after steam drying
Extract；The Sevag reagents are mixed to get according to volume ratio chloroform-n-butanol 3-4: 1.
5. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Herba Plantaginis extract by
Following methods are prepared：After Asiatic plantain rhizome clean dry, 50-100 mesh sieves are crushed, weigh the addition of 100-120g powder
In conical flask, the volume fraction 40-50% ethanol solutions of 500mL are added, the mass concentration for being subsequently added into 10mL is 0.3-0.4g/
The ammonium sulfate of m L, vibration 1 minute after stand 20-25 minutes, 40-50 DEG C of temperature, power 50-60W ultrasound condition under
30min is extracted, is filtered, solution is moved into stratification in separatory funnel.Upper solution is taken, it is i.e. available after being spray-dried
Herba Plantaginis extract.
6. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Radix Berberidis extract by
Following methods are prepared：The barberry rhizome that the root skin for harvesting berberis brachypoda Maxim is obtained takes 200-300g to add triangle after crushing
In bottle, the acetic acid methanol solution of the 1-3% of cumulative volume is added, is carried out 20-25 minutes at 35-40 DEG C, under microwave power 500W
Reaction, be then centrifuged and take solution, after spray drying i.e. can obtain Radix Berberidis extract.
7. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the Stenoloma chusana extract by with
Lower section method is prepared：After Stenoloma chusana drying and crushing, add mass fraction 40-50% ethanol solutions and carry out refluxing extraction, reflux
The condition of extraction is to repeat extraction 2-3 times, 20-30 minutes each, and the temperature of extraction is 50-60 DEG C, will repeat the filtrate of extraction
Carry out being concentrated under reduced pressure into original volume after merging 1.12 times can obtain Stenoloma chusana extract；The volume ratio of Stenoloma chusana and ethanol solution is
1:10-25。
8. the disease-resistant cultural method of the emerald green duck of dragon victory according to claim 1, it is characterised in that the sweetener is xylitol
Or oligoisomaltose.
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| CN103461715A (en) * | 2013-09-27 | 2013-12-25 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Aquatic feed utilizing nano-carrier material as auxiliary material and preparing method for aquatic feed |
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