CN106616024A - Pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving intestinal microenvironment of fishes and preparation method thereof - Google Patents
Pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving intestinal microenvironment of fishes and preparation method thereof Download PDFInfo
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- CN106616024A CN106616024A CN201611118602.2A CN201611118602A CN106616024A CN 106616024 A CN106616024 A CN 106616024A CN 201611118602 A CN201611118602 A CN 201611118602A CN 106616024 A CN106616024 A CN 106616024A
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- pomegranate seed
- powder
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- pomegranate
- fishes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving the intestinal microenvironment of fishes and a preparation method thereof. The feed is applied to fish farming, has the functions of stopping diarrhea, preventing inflammation, and improving the intestinal microenvironment of fishes, and can enhance the immunity and disease resistant performance of fishes, reduce the morbidity, promote the growth of fishes, and increase the food intake. The production is promoted, the feed is safe to use, the feeding effect is good, the weight gain of fishes is quick, the happening rate of diseases is low, and the farming benefits are high.
Description
Technical field
The present invention relates to aquatic fish cultural technique field, more particularly to a kind of antidiarrheal anti-inflammatory and improve fish intestinal microenvironment
Pomegranate seed albumen powder feedstuff and preparation method thereof.
Background technology
Pomegranate is Punicaceae plant, and there is cultivation China north and south various places, and distribution is wider.Xinjiang introducing and planting pomegranate away from
The history in modern at least more than 1600 years.Pomegranates produced in Xinjiang Uygur autonomous resource is enriched very much, and the weather conditions of southern Xinjiang summer are advantageous to
Pomegranate grows, especially the area such as Keshen and field, Aksu and Turfan of South Sinkiang.The superior natural conditions in Xinjiang and
Abundant land resource provides wide development space and huge market potential for pomegranate production, used as the fruit of dietotherapeutic
In king, pomegranates produced in Xinjiang Uygur autonomous is rich in sugar, colour, good quality and pest and disease damage are few, is a kind of green fruit.With national Western Great Development
Send out the industrial restructuring and planning of implementation and autonomous region's Developing Local Special Fruit Trees industry, pomegranate southern Xinjiang cultivation, it is deep plus
Work and industrialization have obtained fast development, while also having driven the development of related discipline.But the pomegranate secondary industry in current Xinjiang
Still in relatively low level, substantial amounts of pomegranate seed（Account for the 20-30% of pomegranate）It is taken as waste material to abandon, causes the serious of resource
Waste.Nutrient content containing various needed by human body in pomegranate, protein content is 14.27% in pomegranates produced in Xinjiang Uygur autonomous seed, wherein containing
18 kinds of amino acid, belong to complete protein.Its Glutamic Acid and arginine content are higher.Very abundant containing K in pomegranate seed, be secondly
Mg and Ca, in addition also more Zn.If it is possible to pomegranate seed albumen is extracted and utilized, it will to Xinjiang
Preferable economic benefit and social benefit are come in pomegranate industrial zone.《The research of pomegranate seed protein extraction technique》Side disclosed in one text
Though method has certain practical value, because its extraction efficiency is low and technique backwardness is rotten, it is impossible to make the leaf protein for extracting
More preferable quality, taste and flavor improvement are obtained, conditioning and regulation to aquatic fish physiological function can not be realized, be unfavorable for
The raising of aquatic fish production performance and culture benefit.Therefore, the present invention has done improvement process, not only increases carrying for leaf protein
Efficiency is taken, leaf protein yield is increased, growth, the production performance of aquatic fish is further improved, the economic benefit of cultivation is improve.
The content of the invention
In order to solve the deficiencies in the prior art, the invention provides a kind of antidiarrheal anti-inflammatory and improving the stone of fish intestinal microenvironment
Pomegranate seed albumen powder feedstuff and preparation method thereof.
The present invention is achieved by the following technical solutions：
A kind of antidiarrheal anti-inflammatory simultaneously improves the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment, is prepared by the raw materials in：
Pomegranate seed 1200-1500, NaOH 1-1.6, hydrochloric acid 2-3, compound protease 0.1-0.15, dried pumpkin 32-36, malt 12-
14th, Chinese bulbul 0.7-0.9, felwort 1.3-1.5, terminalia flesh 1.2-1.4, dark plum powder 7-8, tomato seed protein powder 13-15, the root of Dahurain angelica
Powder 1.2-1.4, green soya bean flour 18-21, persimmon slurry 3-4, calamus powder 0.2-0.3, calcium propionate 0.02-0.03, gluconic acid 1.5-
1.8th, carboxymethylcellulose calcium 0.6-0.8.
Described antidiarrheal anti-inflammatory simultaneously improves the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment, and its preparation method is as follows：
（1）The fresh fruit processing factory most discarded object pomegranate seed of interior output in a couple of days is reclaimed, screen totally and is put into fermentation vat,
Water is filled in fermentation vat, water is drained after immersion 12-16h, changed to new water and enter, diel is soaked again, take out pomegranate seed,
Kind of a skin is peelled off, pomegranate seed is taken out after hardship is gone in 72-78 DEG C of hot water decoction and is crushed, decocting time 3-4h, powder particle diameter
140-160 mesh, by the pomegranate seed powder and water after crushing 1: 10-12 ratio mixing is compared according to weight, is ground to form using colloid mill
Pomegranate seed is starched, and is added to standby in retort, and NaOH is configured into mass concentration for 0.5-0.8% sodium hydroxide solutions, is used
Sodium hydroxide solution adjusts pH value in tank and, to 7.8-8.2,44-46 DEG C of extraction 2-3h, then staticly settles, and is filtered with filter,
Retain filtrate and filter cake, it is standby；
（2）By step（1）The filtrate of reservation is added in retort, then hydrochloric acid is configured into the hydrochloric acid that mass concentration is 10-15%
Solution, adjusts pH value in tank and, to 3.8-4.2, precipitates 30-50min with hydrochloric acid solution, is filtered with filter again, by precipitation washing
To neutral, freeze-drying is carried out, obtain pomegranate seed albumen powder A；
（3）By step（1）The filter cake of reservation is washed with water to neutrality, is then mixed according to the ratio that weight compares 1: 15-18 with water, is stirred
Homogenate is mixed, in being then added to retort, tall can temperature is to 42-44 DEG C and is incubated for liter, then adds compound protease in tank
Stir and evenly mix, enzyme digestion reaction 18-20h, then 15-20min is centrifuged using centrifuge 3000-5000r/min, centrifuged supernatant is dense
Contracing carries out freeze-drying, obtains pomegranate seed albumen powder B；
（4）Dried pumpkin, malt are put into pulverizer and are crushed to 100-120 mesh, heating is steamed and taken out after 30-40min, by hoary hair
Father-in-law, felwort, terminalia flesh mix with 8-10 times of its weight portion and persistently decoct 1-2h, by decoction liquor and dried pumpkin, the wheat crushed
Bud mixes, and 30-35min is heated at a temperature of 84-87 DEG C, then adds dark plum powder, tomato seed protein powder, the root of Dahurain angelica to the inside
Powder mixes, and with ultrasonication 10-15min of 3-5MHz, obtains modified assistant agent；
（5）By step（2）、（3）Pomegranate seed albumen powder A, B of acquisition is mixed, and then adds step to the inside（4）Change
Property assistant agent is mixed again, afterwards again mixes it according to the ratio that weight compares 1: 10-15 with water, in being added to retort,
76-78 DEG C of insulation reaction 2-3h, then add green soya bean flour, persimmon slurry, calamus powder to mix in retort, 104-108 DEG C of insulation
Reaction 10-15min, is then cooled to 54-56 DEG C, and insulation reaction 1-2h takes out reactant liquor, carries out concentrating and freeze-drying, obtains
To modified pomegranate seed albumen powder；
（6）By other surplus stocks and step（5）The modified pomegranate seed albumen powder mixing for obtaining, 64-68 DEG C of modulation 15-20min,
Pass it through and obtain after freeze-drying final finished.
Beneficial effects of the present invention：
The present invention is used in fishery -ies product breeding process with antidiarrheal anti-inflammatory and improves the effect of fish intestinal microenvironment, can strengthen fish
Immunologic function and resistance against diseases, reduce the incidence of disease, moreover it is possible to promote growing for fish, increase food ration, improve production performance, raise
Material is using safety, and feeding effect is good, and fish weightening is fast, and disease is few, and culture benefit is high.The present invention can make what is produced after pomegranate processing
Substantial amounts of pomegranate seed is utilized again, it is to avoid is abandoned and causes the serious waste of resource, realizes pomegranate seed comprehensive exploitation,
Extend processing industry chain, improve processing added value, supplement the deficiency of China's forage protein, reduce dependence of the fish culture industry to fish meal
Property, reduce feeding cost.Pomegranate seed albumen is a kind of vegetable protein of green, and with good application prospect, pomegranate seed is at me
State's yield is huge, is easy to large-scale production, therefore, its economic benefit of Devoting Major Efforts To Developing pomegranate seed and social benefit are very big.The present invention
Also there is greatly breakthrough in preparation technology, relative to traditional technology of preparing, it is an advantage of the current invention that by by discarded object
Pomegranate seed is reclaimed, and first soaks diel to it, to expand seed, is easy to peel off kind of a skin, is then carried out decoction to it and is gone hardship,
Because bitter taste can affect the appetite of fish, extracting protein again after hardship is carried out to pomegranate seed can be greatly improved the product of albumen powder
Matter, by the second extraction using the heavy first extraction of alkali soluble acid and enzymatic isolation method during extraction protein, greatly improves pomegranate seed
The extraction efficiency and yield of albumen, malt, the Chinese bulbul, terminalia flesh, dark plum powder, tomato seed protein powder, root of Dahurain angelica powder etc. with cooperation
For the assistant agent that is modified, nutrition and the gain-of-function supplement of pomegranate seed albumen can not only be made, moreover it is possible to improve quality, the mouth of protein feed
Sense and growth, the production performance of fish, so as to improve culture benefit.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention, rather than the embodiment of whole, and based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
A kind of antidiarrheal anti-inflammatory simultaneously improves the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment, by following weight portion（kg）Original
Material is made：Pomegranate seed 1200, NaOH 1, hydrochloric acid 2, compound protease 0.1, dried pumpkin 32, malt 12, the Chinese bulbul 0.7, dragon
Courage grass 1.3, terminalia flesh 1.2, dark plum powder 7, tomato seed protein powder 13, root of Dahurain angelica powder 1.2, green soya bean flour 18, persimmon slurry 3, calamus powder
0.2nd, calcium propionate 0.02, gluconic acid 1.5, carboxymethylcellulose calcium 0.6.
Described antidiarrheal anti-inflammatory simultaneously improves the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment, and its preparation method is as follows：
（1）The fresh fruit processing factory most discarded object pomegranate seed of interior output in a couple of days is reclaimed, screen totally and is put into fermentation vat,
Water is filled in fermentation vat, water is drained after immersion 12h, changed to new water and enter, diel is soaked again, take out pomegranate seed, stripping
Kind of a skin is removed, pomegranate seed is taken out after hardship is gone in 72 DEG C of hot water decoction and is crushed, decocting time 3h, the mesh of powder particle diameter 140 will
Pomegranate seed powder and water after crushing compares 1: 10 ratio mixing according to weight, and using colloid mill pomegranate seed slurry is ground to form, and is added to
Standby in retort, it is 0.5% sodium hydroxide solution that NaOH is configured into mass concentration, and with sodium hydroxide solution tank is adjusted
Interior pH value is to 7.8, and then 44 DEG C of extraction 2h staticly settle, and are filtered with filter, retain filtrate and filter cake, standby；
（2）By step（1）The filtrate of reservation is added in retort, then that hydrochloric acid is configured into the hydrochloric acid that mass concentration is 10% is molten
Liquid, adjusts pH value in tank and, to 3.8, precipitates 30min with hydrochloric acid solution, is filtered with filter again, and precipitation is washed into neutrality, enters
Row freeze-drying, obtains pomegranate seed albumen powder A；
（3）By step（1）The filter cake of reservation is washed with water to neutrality, is then mixed according to the ratio that weight compares 1: 15 with water, stirring
Into homogenate, in being then added to retort, tall can temperature is to 42 DEG C and is incubated for liter, then adds compound protease stirring mixed in tank
It is even, enzyme digestion reaction 18h, then 15min is centrifuged using centrifuge 3000r/min, centrifuged supernatant is concentrated and is carried out freeze-drying,
Obtain pomegranate seed albumen powder B；
（4）Dried pumpkin, malt are put into pulverizer and are crushed to 100 mesh, heating is steamed and taken out after 30min, by the Chinese bulbul, rough gentian
Grass, terminalia flesh mix with 8 times of its weight portion and persistently decoct 1h, decoction liquor are mixed with the dried pumpkin, malt crushed, at 84 DEG C
At a temperature of heat 30min, then to the inside add dark plum powder, tomato seed protein powder, the root of Dahurain angelica powder mixing, with the ultrasound of 3MHz
Ripple processes 10min, obtains modified assistant agent；
（5）By step（2）、（3）Pomegranate seed albumen powder A, B of acquisition is mixed, and then adds step to the inside（4）Change
Property assistant agent is mixed again, afterwards again mixes it according to the ratio that weight compares 1: 10 with water, in being added to retort, 76 DEG C
Insulation reaction 2h, then add green soya bean flour, persimmon slurry, calamus powder to mix in retort, 104 DEG C of insulation reactions 10min, then
54 DEG C are cooled to, insulation reaction 1h takes out reactant liquor, carries out concentrating and freeze-drying, obtain modified pomegranate seed albumen powder；
（6）By other surplus stocks and step（5）The modified pomegranate seed albumen powder mixing for obtaining, 64 DEG C of modulation 15min, by its Jing
Cross after freeze-drying and obtain final finished.
Culture experiment is done using feed of the present invention, control group carries out feeding using common daily ration, and test group is using addition 10%
The common daily ration of feed of the present invention carries out feeding, and in addition, other external conditions are identical.Test result indicate that, test
The speed of growth of group fish is significantly faster than that control group, and disease resistance is higher, and survival rate is greatly improved, and cultured output is high, fish development shape
State is good.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not restricted to the described embodiments, the simply explanation described in above-described embodiment and specification this
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes
Change and improvement is both fallen within scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent thereof.
Claims (2)
1. a kind of antidiarrheal anti-inflammatory and the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment is improved, it is characterised in that by following weight
The raw material of part is made：Pomegranate seed 1200-1500, NaOH 1-1.6, hydrochloric acid 2-3, compound protease 0.1-0.15, dried pumpkin
32-36, malt 12-14, Chinese bulbul 0.7-0.9, felwort 1.3-1.5, terminalia flesh 1.2-1.4, dark plum powder 7-8, Tomato Seed egg
White powder 13-15, root of Dahurain angelica powder 1.2-1.4, green soya bean flour 18-21, persimmon slurry 3-4, calamus powder 0.2-0.3, calcium propionate 0.02-
0.03rd, gluconic acid 1.5-1.8, carboxymethylcellulose calcium 0.6-0.8.
2. antidiarrheal anti-inflammatory according to claim 1 and the pomegranate seed albumen powder feedstuff of fish intestinal microenvironment is improved, its preparation
Method is as follows：
（1）The fresh fruit processing factory most discarded object pomegranate seed of interior output in a couple of days is reclaimed, screen totally and is put into fermentation vat,
Water is filled in fermentation vat, water is drained after immersion 12-16h, changed to new water and enter, diel is soaked again, take out pomegranate seed,
Kind of a skin is peelled off, pomegranate seed is taken out after hardship is gone in 72-78 DEG C of hot water decoction and is crushed, decocting time 3-4h, powder particle diameter
140-160 mesh, by the pomegranate seed powder and water after crushing 1: 10-12 ratio mixing is compared according to weight, is ground to form using colloid mill
Pomegranate seed is starched, and is added to standby in retort, and NaOH is configured into mass concentration for 0.5-0.8% sodium hydroxide solutions, is used
Sodium hydroxide solution adjusts pH value in tank and, to 7.8-8.2,44-46 DEG C of extraction 2-3h, then staticly settles, and is filtered with filter,
Retain filtrate and filter cake, it is standby；
（2）By step（1）The filtrate of reservation is added in retort, then hydrochloric acid is configured into the hydrochloric acid that mass concentration is 10-15%
Solution, adjusts pH value in tank and, to 3.8-4.2, precipitates 30-50min with hydrochloric acid solution, is filtered with filter again, by precipitation washing
To neutral, freeze-drying is carried out, obtain pomegranate seed albumen powder A；
（3）By step（1）The filter cake of reservation is washed with water to neutrality, is then mixed according to the ratio that weight compares 1: 15-18 with water, is stirred
Homogenate is mixed, in being then added to retort, tall can temperature is to 42-44 DEG C and is incubated for liter, then adds compound protease in tank
Stir and evenly mix, enzyme digestion reaction 18-20h, then 15-20min is centrifuged using centrifuge 3000-5000r/min, centrifuged supernatant is dense
Contracing carries out freeze-drying, obtains pomegranate seed albumen powder B；
（4）Dried pumpkin, malt are put into pulverizer and are crushed to 100-120 mesh, heating is steamed and taken out after 30-40min, by hoary hair
Father-in-law, felwort, terminalia flesh mix with 8-10 times of its weight portion and persistently decoct 1-2h, by decoction liquor and dried pumpkin, the wheat crushed
Bud mixes, and 30-35min is heated at a temperature of 84-87 DEG C, then adds dark plum powder, tomato seed protein powder, the root of Dahurain angelica to the inside
Powder mixes, and with ultrasonication 10-15min of 3-5MHz, obtains modified assistant agent；
（5）By step（2）、（3）Pomegranate seed albumen powder A, B of acquisition is mixed, and then adds step to the inside（4）Change
Property assistant agent is mixed again, afterwards again mixes it according to the ratio that weight compares 1: 10-15 with water, in being added to retort,
76-78 DEG C of insulation reaction 2-3h, then add green soya bean flour, persimmon slurry, calamus powder to mix in retort, 104-108 DEG C of insulation
Reaction 10-15min, is then cooled to 54-56 DEG C, and insulation reaction 1-2h takes out reactant liquor, carries out concentrating and freeze-drying, obtains
To modified pomegranate seed albumen powder；
（6）By other surplus stocks and step（5）The modified pomegranate seed albumen powder mixing for obtaining, 64-68 DEG C of modulation 15-20min,
Pass it through and obtain after freeze-drying final finished.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611118602.2A CN106616024A (en) | 2016-12-08 | 2016-12-08 | Pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving intestinal microenvironment of fishes and preparation method thereof |
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|---|---|---|---|
| CN201611118602.2A CN106616024A (en) | 2016-12-08 | 2016-12-08 | Pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving intestinal microenvironment of fishes and preparation method thereof |
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| CN201611118602.2A Pending CN106616024A (en) | 2016-12-08 | 2016-12-08 | Pomegranate seed protein powder feed capable of stopping diarrhea, preventing inflammation, and improving intestinal microenvironment of fishes and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912363A (en) * | 2017-11-24 | 2018-04-17 | 桂林满梓玉农业开发有限公司 | A kind of disease-resistant cultural method of the emerald green duck of dragon victory |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936276A (en) * | 2012-11-02 | 2013-02-20 | 石河子大学 | Method for extracting pomegranate seed protein |
| CN103947852A (en) * | 2014-03-17 | 2014-07-30 | 青阳县曙光生态养殖专业合作社 | Grass carp formula feed and its preparation method |
-
2016
- 2016-12-08 CN CN201611118602.2A patent/CN106616024A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936276A (en) * | 2012-11-02 | 2013-02-20 | 石河子大学 | Method for extracting pomegranate seed protein |
| CN103947852A (en) * | 2014-03-17 | 2014-07-30 | 青阳县曙光生态养殖专业合作社 | Grass carp formula feed and its preparation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912363A (en) * | 2017-11-24 | 2018-04-17 | 桂林满梓玉农业开发有限公司 | A kind of disease-resistant cultural method of the emerald green duck of dragon victory |
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