CN107941679A - A kind of reagent for hemocyte analyzers - Google Patents
A kind of reagent for hemocyte analyzers Download PDFInfo
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- CN107941679A CN107941679A CN201711177759.7A CN201711177759A CN107941679A CN 107941679 A CN107941679 A CN 107941679A CN 201711177759 A CN201711177759 A CN 201711177759A CN 107941679 A CN107941679 A CN 107941679A
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- reagent
- hemolytic agents
- hemocyte analyzers
- deionized water
- sodium
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/12—Coulter-counters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The invention discloses a kind of reagent for hemocyte analyzers, it includes dilution, 1 hemolytic agents of CY LY, 2 hemolytic agents of CY LY, 3 hemolytic agents of CY LY, cleaning solution.The reagent for hemocyte analyzers of the present invention can step the replacement reagent of the original-pack reagents of DH76 as the classification cytoanalyze Supreme Being of the prior art five, the problem of original-pack reagent cost is high, delivery cycle is long can be efficiently solved, and accuracy it is high, it is reproducible, with genuine reagent linear regression coefficient R up to more than 0.9900, the preferable correlation of system；The raw material sources of the present invention are extensive, are easy to get, and cheap, nontoxic, environmental pollution is few, the simple production process of product, is easy to duplication of production, and stability is good, long shelf-life, can effectively reduce medical treatment cost, are worth further genralrlization.
Description
Technical field
The present invention relates to medical diagnosis instrument reagent, and in particular to a kind of reagent for hemocyte analyzers.
Background technology
The Hematometer product influence power of Di Mai companies slowly expands in recent years, also obtains the accreditation of laboratory medicine on-Line review, its
Middle DH76 is that a full-automatic sample introduction five is classified blood cell analyzer, it is possible to provide 29 parameters, detection speed is fast, and per hour 90
A sample, automatic platform can once load 60 samples, and compatible a variety of blood sample patterns, support open, two kinds of input modes of closing,
Emergency treatment sample can be inserted at any time, and blood using amount is few, and the classification of binary channels leucocyte five only needs 20 μ l blood, can meet most clinical real
The job requirement of room is tested, is had a extensive future.
But Supreme Being steps the original package reagents of DH76 cellanalyzers and is in great demand at present, due to the reagent cost and
It is expensive, cause medical treatment cost height.
The content of the invention
In view of the problems of the existing technology, the present invention provides a kind of reagent for hemocyte analyzers, particularly Supreme Being to step
DH76 cellanalyzer replacement reagents.
The present invention uses following technical scheme：
A kind of reagent for hemocyte analyzers, it includes dilution, CY-LY-1 hemolytic agents, CY-LY-2 hemolytic agents, CY-
LY-3 hemolytic agents, cleaning solution；
The dilution include 3.5~8.0g/L of sodium chloride, 3.5~8.0g/L of sodium sulphate, disodium hydrogen phosphate 2.0~
6.0g/L, 0.1~3.0g/L of potassium dihydrogen phosphate, 0.2~1.0g/L of EDTA-2Na, 0.3~1.0g/L of preservative；
The CY-LY-1 hemolytic agents include 5.0~20.0g/L of decyl trimethyl ammonium chloride, 1.0~5.0g/ of Brij35
L, 2.0~7.0g/L of formic acid, 10.0~25.0g/L of sodium chloride；
The CY-LY-2 hemolytic agents include 1.0~4.0g/L of dodecyl trimethyl ammonium chloride, 1.0~4.0g/ of OP-10
L, 1.0~5.0g/L of maleic acid, 2.0~5.0g/L of hydrogen phthalate sodium, 1.0~4.0g/L of sodium chloride；
The CY-LY-3 hemolytic agents include eight 1.0~3.0g/L of alkyl trimethyl ammonium chloride, 1.0~4.0g/L of OP-10,
2.0~5.0g/L of PEG2000,1.0~5.0g/L of maleic acid, 2.0~5.0g/L of hydrogen phthalate sodium, sodium chloride 2.0~
6.0g/L, 0.5~2.0g/L of methyl green；
The cleaning solution includes 0.5~2.0g/L of TritionX-100, wherein liquor natrii hypochloritis, liquor natrii hypochloritis
Effective chlorine ＞ 5%.
Further, the pH value of the dilution is 6.5~7.2, and the electrical conductivity of the dilution is 15.0~19.0ms/
Cm, the osmotic pressure of the dilution is 260~330mOsm/kg.
Further, sequentially added in 1000mL beakers the disodium hydrogen phosphate weighed, potassium dihydrogen phosphate, sodium chloride,
Sodium sulphate, EDTA-2Na, add suitable deionized water, and heating stirring is cooled to less than 40 DEG C after it is completely dissolved, and adds
The preservative weighed up, stirs evenly, and with the volume of deionized water constant volume to 1L, measures the pH value, electrical conductivity, infiltration of solution
Pressure, up to the dilution after filtering.
Further, the pH value of the CY-LY-1 hemolytic agents is 2.0~3.0, the electrical conductivity of the CY-LY-1 hemolytic agents
For 35.0~40.0ms/cm, the osmotic pressure of the CY-LY-1 hemolytic agents is 700~800mOsm/kg.
Further, the decyl trimethyl ammonium chloride weighed, Brij35, chlorination are sequentially added in 1000mL beakers
Sodium, adds suitable deionized water, and heating stirring adds formic acid, with the body of deionized water constant volume to 1L after it is completely dissolved
Product, stirs evenly, measures the pH value, electrical conductivity, osmotic pressure of solution, up to the CY-LY-1 hemolytic agents after filtering.
Further, the pH value of the CY-LY-2 hemolytic agents is 5.50~6.0, the electrical conductivity of the CY-LY-2 hemolytic agents
For 6.0~7.0ms/cm, the osmotic pressure of the CY-LY-2 hemolytic agents is 100~150mOsm/kg.
Further, the dodecyl trimethyl ammonium chloride weighed, OP-10, horse are sequentially added in 1000mL beakers
Come sour, hydrogen phthalate sodium, sodium chloride, add suitable deionized water, heating stirring is completely dissolved it, uses deionized water
Constant volume stirs evenly to the volume of 1L, measures the pH value, electrical conductivity, osmotic pressure of solution, the CY-LY-2 is molten to obtain the final product after filtering
Blood agent.
Further, the pH value of the CY-LY-3 hemolytic agents is 5.50~6.0, the electrical conductivity of the CY-LY-3 hemolytic agents
For 9.0~10.0ms/cm, the osmotic pressure of the CY-LY-3 hemolytic agents is 200~250mOsm/kg.
Further, sequentially added in 1000mL beakers eight alkyl trimethyl ammonium chlorides weighed, OP-10,
PEG2000, maleic acid, hydrogen phthalate sodium, sodium chloride, add suitable deionized water, and heating stirring is completely dissolved it,
Methyl green is added after cooling, stirring and dissolving, with the volume of deionized water constant volume to 1L, stirs evenly, up to CY-LY-3 after filtering
Hemolytic agent.
Further, the pH value of the cleaning solution is 10.0~12.0, the electrical conductivity of the cleaning solution for 25.0~
30.0ms/cm。
Further, the TritionX-100 weighed is sequentially added in 1000mL beakers, adds suitable deionization
Water, heating stirring are completely dissolved it.The liquor natrii hypochloritis weighed up is added after cooling, stir evenly add it is remaining go from
Sub- water constant volume measures pH value, the electrical conductivity of solution to the volume of 1L, up to cleaning solution or probe cleaning solution after filtering.
The present invention have developed a whole set of Supreme Being and step DH76 cellanalyzer replacement reagents.
The dilution be blood cell analysis with dilution, the CY-LY-1 hemolytic agents be blood cell analysis hemolytic agent,
The CY-LY-2 hemolytic agents be blood cell analysis with hemolysate, the CY-LY-3 hemolytic agents be blood cell analysis hemolysate,
The cleaning solution is blood cell analysis cleaning solution or probe cleaning solution.
Current advanced five sorting technique of haemocyte, it is thin to employ Coulter principle, colorimetric method and semiconductor laser streaming
Born of the same parents' technology, can detect red blood cell, hemoglobin, blood platelet, leucocyte five classify, the parameter such as mean corpuscular volume (MCV), pass through
The number of detection human body haemocyte judges various diseases, monitors physical condition in time, reduces disease incidence probability or control
The state of an illness processed.
Need to be used together with matched reagent during the classification Blood cell analyzer detection blood cell numbers of DH76 five, matched reagent
Major class includes dilution, hemolytic agent, cleaning solution.The main function of dilution is to provide osmotic pressure identical with blood plasma, an electricity
The environment of conductance, LYA-2 hemolytic agents, LYA-3 hemolytic agents are lysed erythrocytes, keep leucocyte integrality, and leucocyte is carried out
Classification and counting, LYA-1 hemolytic agents are lysed erythrocytes, discharge hemoglobin, form stable haemoglobin dervative, into
The measure of promoting circulation of blood hemoglobin concentration；The instrument is not equipped with cleaning solution, serves as cleaning solution with dilution, main function is cleaning pipe
Road, mutually pollutes between sample；Cleaning when probe cleaning solution is exactly shutdown or maintenance to instrument pipeline, specimen needle, avoids pipe
Road blocks.
Beneficial effects of the present invention：
(1) product of the invention can step the replacement of the original-pack reagents of DH76 as the classification cytoanalyze Supreme Being of the prior art five
Reagent, can efficiently solve the problem of of high cost, delivery cycle is long；
(2) matched reagent accuracy of the invention it is high, it is reproducible, reached with genuine reagent linear regression coefficient R
More than 0.9900, system has preferable correlation；
(3) raw material sources of the invention are extensive, are easy to get, and cheap, nontoxic, environmental pollution is few；
(4) simple production process of product of the present invention, is easy to duplication of production, stability is good, long shelf-life；
(5) price of the invention can effectively reduce medical treatment cost, be worth further less than original-pack reagent up to 30% or so
It is widely popularized.
Brief description of the drawings
Fig. 1 is the test result contrast that reagent made from the embodiment of the present invention 1 steps the WBC projects that reagent is surveyed respectively with Supreme Being
Correlation linear graph；
Fig. 2 is the test result contrast that reagent made from the embodiment of the present invention 1 steps the RBC projects that reagent is surveyed respectively with Supreme Being
Correlation linear graph；
Fig. 3 is the test result contrast that reagent made from the embodiment of the present invention 1 steps the HGB projects that reagent is surveyed respectively with Supreme Being
Correlation linear graph；
Fig. 4 is the test result contrast that reagent made from the embodiment of the present invention 1 steps the MCV projects that reagent is surveyed respectively with Supreme Being
Correlation linear graph；
Fig. 5 is the test result contrast that reagent made from the embodiment of the present invention 1 steps the PLT projects that reagent is surveyed respectively with Supreme Being
Correlation linear graph.
Embodiment
In order to preferably explain the present invention, it is described further in conjunction with specific examples below, but the present invention is unlimited
In specific embodiment.
Instrument：Supreme Being steps DH76 cellanalyzers, 1；
Supreme Being steps genuine reagent：Blood cell analysis dilution, LYA-1 hemolytic agents, LYA-2 hemolytic agents, LYA-3 hemolytic agents,
Probe cleaning solution；
Reagent of the present invention：Dilution, CY-LY-1 hemolytic agents, CY-LY-2 hemolytic agents, CY-LY-3 hemolytic agents, cleaning solution.
Embodiment 1
DH76 cellanalyzers replacement reagent of the present invention
(1) formula of dilution of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the phosphoric acid hydrogen two weighed is sequentially added in 1000mL beakers
Sodium, potassium dihydrogen phosphate, sodium chloride, sodium sulphate, EDTA-2Na, add suitable deionized water, and heating stirring is completely dissolved it.
After material is completely dissolved, less than 40 DEG C are cooled to, the preservative weighed up is added, stirs evenly, the volume of constant volume to 1L, is measured
The pH value of solution is 6.87, electrical conductivity 16.94ms/cm, osmotic pressure 290mOsm/kg, up to dilution after filtering.
(2) formula of CY-LY-1 hemolytic agents of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the ten alkyl front threes weighed are sequentially added in 1000mL beakers
Ammonium chloride, Brij35, sodium chloride, add suitable deionized water, and heating stirring is completely dissolved it, add formic acid and residue
Deionized water, the volume of constant volume to 1L, stirs evenly, measure solution pH value be 2.20, electrical conductivity 36.10ms/cm, oozes
Pressure is 761mOsm/kg thoroughly, up to CY-LY-1 hemolytic agents after filtering.
(3) formula of CY-LY-2 hemolytic agents of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the dodecyl three weighed is sequentially added in 1000mL beakers
Ammonio methacrylate, OP-10, maleic acid, hydrogen phthalate sodium, sodium chloride, add suitable deionized water, and heating stirring makes it
It is completely dissolved, adds remaining deionized water, the volume of constant volume to 1L, stirs evenly, and the pH value for measuring solution is 5.60, conductance
Rate is 6.21ms/cm, osmotic pressure 124mOsm/kg, up to CY-LY-2 hemolytic agents after filtering.
(4) formula of CY-LY-3 hemolytic agents of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the eight alkyl front threes weighed are sequentially added in 1000mL beakers
Ammonium chloride, OP-10, PEG2000, maleic acid, hydrogen phthalate sodium, sodium chloride, add suitable deionized water, and heating is stirred
Mixing is completely dissolved it, methyl green is added after cooling, stirring and dissolving, adds remaining deionized water, the volume of constant volume to 1L, is stirred
Mix uniformly, the pH value for measuring solution is 5.54, electrical conductivity 9.23ms/cm, osmotic pressure 214mOsm/kg, after filtering to obtain the final product
CY-LY-3 hemolytic agents.
(5) formula of cleaning solution of the present invention：
TritionX-100 1.5g/L
Liquor natrii hypochloritis 700mL/L (liquor natrii hypochloritis's effective chlorine is 7.5%)
Required material is accurately weighed by above-mentioned formula ratio, the TritionX- weighed is sequentially added in 1000mL beakers
100, suitable deionized water is added, heating stirring is completely dissolved it.The liquor natrii hypochloritis weighed up is added after cooling, is stirred
The volume for being uniformly added into remaining deionized water constant volume to 1L is mixed, the pH value for measuring solution is 11.0, electrical conductivity 28.0ms/
Cm, up to probe cleaning solution after filtering.
Experimental method and result：
DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 and Supreme Being are stepped into original-pack DH76 blood cell analysis
Instrument reagent according to NCCLS files EP9-A2 (American National Clinical Laboratory Standard committee paper approval guide-second edition-
Carry out method with patient's sample and compare bias evaluation) method to same 100 (monstrosity containing WBC) fresh blood sample tests,
100 groups of data are obtained, data are listed in Table 1 below, and original-pack reagent for hemocyte analyzers test result is stepped as abscissa using Supreme Being, with
DH76 cellanalyzers replacement reagent test result is ordinate made from the embodiment of the present invention 1, is done by Origin softwares
Linear regression, obtains reagent made from the embodiment of the present invention 1 of Fig. 1 and steps the test knot for the WBC projects that reagent is surveyed respectively with Supreme Being
Fruit contrasts correlation linear graph, and linear regression is drawn by the WBC project testing data of table 1：Y=1.00959X+0.03378, R=
0.99872；
DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 and Supreme Being are stepped into original-pack DH76 blood cell analysis
Instrument reagent according to NCCLS files EP9-A2 (American National Clinical Laboratory Standard committee paper approval guide-second edition-
Carry out method with patient's sample and compare bias evaluation) method to same 100 (monstrosity containing RBC) fresh blood sample tests,
100 groups of data are obtained, data are listed in Table 1 below, and original-pack reagent for hemocyte analyzers test result is stepped as abscissa using Supreme Being, with
DH76 cellanalyzers replacement reagent test result is ordinate made from the embodiment of the present invention 1, is done by Origin softwares
Linear regression, obtains reagent made from the embodiment of the present invention 1 of Fig. 2 and steps the test knot for the RBC projects that reagent is surveyed respectively with Supreme Being
Fruit contrasts correlation linear graph, and linear regression is drawn by the RBC project testing data of table 1：Y=0.99411X+0.0137, R=
0.99553；
DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 and Supreme Being are stepped into original-pack DH76 blood cell analysis
Instrument reagent according to NCCLS files EP9-A2 (American National Clinical Laboratory Standard committee paper approval guide-second edition-
Carry out method with patient's sample and compare bias evaluation) method to same 100 (monstrosity containing HGB) fresh blood sample tests,
100 groups of data are obtained, data are listed in Table 1 below, and original-pack reagent for hemocyte analyzers test result is stepped as abscissa using Supreme Being, with
DH76 cellanalyzers replacement reagent test result is ordinate made from the embodiment of the present invention 1, is done by Origin softwares
Linear regression, obtains reagent made from the embodiment of the present invention 1 of Fig. 3 and steps the test knot for the HGB projects that reagent is surveyed respectively with Supreme Being
Fruit contrasts correlation linear graph, and linear regression is drawn by the HGB project testing data of table 1：Y=0.9966X+0.72619, R=
0.99648；
DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 and Supreme Being are stepped to original-pack DH76 haemocytes point
According to NCCLS files EP9-A2, (American National Clinical Laboratory Standard committee paper ratifies guide-the second to analyzer reagent
Version-with patient's sample carries out method comparison bias evaluation) method to same 100 (monstrosities containing MCV) fresh blood specimen survey
Examination, obtains 100 groups of data, data are listed in Table 1 below, and original-pack reagent for hemocyte analyzers test result is stepped as horizontal seat using Supreme Being
Mark is soft by Origin as ordinate using DH76 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Part does linear regression, obtains reagent made from the embodiment of the present invention 1 of Fig. 4 and steps the survey for the MCV projects that reagent is surveyed respectively with Supreme Being
Test result contrasts correlation linear graph, and linear regression is drawn by the MCV project testing data of table 1：Y=0.95817X+4.3734,
R=0.98264；
DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 and Supreme Being are stepped into original-pack DH76 blood cell analysis
Instrument reagent according to NCCLS files EP9-A2 (American National Clinical Laboratory Standard committee paper approval guide-second edition-
Carry out method with patient's sample and compare bias evaluation) method to same 100 (monstrosity containing PLT) fresh blood sample tests,
100 groups of data are obtained, data are listed in Table 1 below, and original-pack reagent for hemocyte analyzers test result is stepped as abscissa using Supreme Being, with
DH76 cellanalyzers replacement reagent test result is ordinate made from the embodiment of the present invention 1, is done by Origin softwares
Linear regression, obtains reagent made from the embodiment of the present invention 1 of Fig. 5 and steps the test knot for the PLT projects that reagent is surveyed respectively with Supreme Being
Fruit contrasts correlation linear graph, and linear regression is drawn by the PLT project testing data of table 1：Y=1.00877X-2.02855, R=
0.99519；
Table 1：DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1 steps the original-pack blood cell analysis of DH with Supreme Being
(wherein (i) steps the original-pack cytoanalyzes of DH76 to the result of the test for WBC, RBC, HGB, MCV, PLT project that instrument reagent place surveys for Supreme Being
The data of reagent, (I) are the data of DH76 cellanalyzers replacement reagent made from the embodiment of the present invention 1).
The foregoing is merely the specific embodiment of the present invention, it is not intended to limit the scope of the invention, every utilization
The equivalent transformation that the present invention makees, is directly or indirectly used in other relevant technical fields, is similarly included in the present invention's
Among scope of patent protection.
Claims (10)
1. a kind of reagent for hemocyte analyzers, it is characterised in that it is molten that it includes dilution, CY-LY-1 hemolytic agents, CY-LY-2
Blood agent, CY-LY-3 hemolytic agents, cleaning solution；
The dilution include 3.5~8.0g/L of sodium chloride, 3.5~8.0g/L of sodium sulphate, 2.0~6.0g/L of disodium hydrogen phosphate,
0.1~3.0g/L of potassium dihydrogen phosphate, 0.2~1.0g/L of EDTA-2Na, 0.3~1.0g/L of preservative；
The CY-LY-1 hemolytic agents include 5.0~20.0g/L of decyl trimethyl ammonium chloride, 1.0~5.0g/L of Brij35, first
2.0~7.0g/L of acid, 10.0~25.0g/L of sodium chloride；
The CY-LY-2 hemolytic agents include 1.0~4.0g/L of dodecyl trimethyl ammonium chloride, 1.0~4.0g/L of OP-10, horse
Come sour 1.0~5.0g/L, 2.0~5.0g/L of hydrogen phthalate sodium, 1.0~4.0g/L of sodium chloride；
The CY-LY-3 hemolytic agents include eight 1.0~3.0g/L of alkyl trimethyl ammonium chloride, 1.0~4.0g/L of OP-10,
2.0~5.0g/L of PEG2000,1.0~5.0g/L of maleic acid, 2.0~5.0g/L of hydrogen phthalate sodium, sodium chloride 2.0~
6.0g/L, 0.5~2.0g/L of methyl green；
The cleaning solution includes 0.5~2.0g/L of TritionX-100, liquor natrii hypochloritis.
2. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the dilution is 6.5
~7.2, the electrical conductivity of the dilution is 15.0~19.0ms/cm, and the osmotic pressure of the dilution is 260~330mOsm/
kg。
3. reagent for hemocyte analyzers according to claim 1 or 2, it is characterised in that title is sequentially added in beaker
Disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, sodium sulphate, the EDTA-2Na taken, adds deionized water, and heating stirring treats it
After being completely dissolved, less than 40 DEG C are cooled to, the preservative weighed up is added, stirs evenly, with the body of deionized water constant volume to 1L
Product, up to the dilution after filtering.
4. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH of the CY-LY-1 hemolytic agents
It is worth for 2.0~3.0, the electrical conductivity of the CY-LY-1 hemolytic agents is 35.0~40.0ms/cm, and the CY-LY-1 hemolytic agents ooze
Pressure is 700~800mOsm/kg thoroughly.
5. the reagent for hemocyte analyzers according to claim 1 or 4, it is characterised in that title is sequentially added in beaker
Decyl trimethyl ammonium chloride, Brij35, the sodium chloride taken, adds deionized water, and heating stirring adds after it is completely dissolved
Enter formic acid, with the volume of deionized water constant volume to 1L, stir evenly, up to the CY-LY-1 hemolytic agents after filtering.
6. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH of the CY-LY-2 hemolytic agents
It is worth for 5.50~6.0, the electrical conductivity of the CY-LY-2 hemolytic agents is 6.0~7.0ms/cm, and the CY-LY-2 hemolytic agents ooze
Pressure is 100~150mOsm/kg thoroughly.
7. the reagent for hemocyte analyzers according to claim 1 or 6, it is characterised in that title is sequentially added in beaker
The dodecyl trimethyl ammonium chloride that has taken, OP-10, maleic acid, hydrogen phthalate sodium, sodium chloride, add deionized water, add
Thermal agitation is completely dissolved it, with the volume of deionized water constant volume to 1L, stirs evenly, molten up to the CY-LY-2 after filtering
Blood agent.
8. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH of the CY-LY-3 hemolytic agents
It is worth for 5.50~6.0, the electrical conductivity of the CY-LY-3 hemolytic agents is 9.0~10.0ms/cm, and the CY-LY-3 hemolytic agents ooze
Pressure is 200~250mOsm/kg thoroughly.
9. the reagent for hemocyte analyzers according to claim 1 or 8, it is characterised in that title is sequentially added in beaker
Eight alkyl trimethyl ammonium chlorides, OP-10, PEG2000, maleic acid, hydrogen phthalate sodium, the sodium chloride taken, addition go from
Sub- water, heating stirring are completely dissolved it, then add methyl green, stirring and dissolving, with the volume of deionized water constant volume to 1L, is stirred
Mix uniformly, up to CY-LY-3 hemolytic agents after filtering.
10. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the cleaning solution is
10.0~12.0, the electrical conductivity of the cleaning solution is 25.0~30.0ms/cm.
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| CN201711177759.7A CN107941679A (en) | 2017-11-22 | 2017-11-22 | A kind of reagent for hemocyte analyzers |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655604A (en) * | 2018-12-27 | 2019-04-19 | 山东博科生物产业有限公司 | A set of universal three classification blood cell analysis reagent |
| CN111006990A (en) * | 2019-12-31 | 2020-04-14 | 山东博科生物产业有限公司 | Reagent for analyzing blood cells and use thereof |
| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
| CN113008653A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Diluent, hemocyte analyzer, reagent for hemocyte analyzer, and kit |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037966A (en) * | 1988-01-29 | 1989-12-13 | 阿盖尔钻石销售有限公司 | Sorting and/or examine the method and apparatus of jewel homogeneity |
| CN101819199A (en) * | 2010-05-08 | 2010-09-01 | 桂林市朗道诊断用品有限公司 | Reagent for hemocyte analyzers |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN103323581A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Hemocyte analyzer hemolytic agent |
| CN104698157A (en) * | 2015-02-13 | 2015-06-10 | 中山市创艺生化工程有限公司 | Agent for blood cell analyzer |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
| CN107144519A (en) * | 2017-05-24 | 2017-09-08 | 中山市滔略生物科技有限公司 | Cellanalyzer hemolytic agent and preparation method thereof |
-
2017
- 2017-11-22 CN CN201711177759.7A patent/CN107941679A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037966A (en) * | 1988-01-29 | 1989-12-13 | 阿盖尔钻石销售有限公司 | Sorting and/or examine the method and apparatus of jewel homogeneity |
| CN101819199A (en) * | 2010-05-08 | 2010-09-01 | 桂林市朗道诊断用品有限公司 | Reagent for hemocyte analyzers |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN103323581A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Hemocyte analyzer hemolytic agent |
| CN104698157A (en) * | 2015-02-13 | 2015-06-10 | 中山市创艺生化工程有限公司 | Agent for blood cell analyzer |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
| CN107144519A (en) * | 2017-05-24 | 2017-09-08 | 中山市滔略生物科技有限公司 | Cellanalyzer hemolytic agent and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 国际贸易网: "《国产五分类+CRP全自动血液细胞分析仪 帝迈D7-CRP血球》", 《国产五分类+CRP全自动血液细胞分析仪 帝迈D7-CRP血球》 * |
| 最初的孤单: "《帝迈D7-CRP血细胞分析仪》", 《帝迈D7-CRP血细胞分析仪》 * |
| 通知公告: "《甘肃中医药大学定西校区购置检验专业实验耗材招标公告》", 《甘肃中医药大学定西校区购置检验专业实验耗材招标公告》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655604A (en) * | 2018-12-27 | 2019-04-19 | 山东博科生物产业有限公司 | A set of universal three classification blood cell analysis reagent |
| CN113008653A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Diluent, hemocyte analyzer, reagent for hemocyte analyzer, and kit |
| CN111006990A (en) * | 2019-12-31 | 2020-04-14 | 山东博科生物产业有限公司 | Reagent for analyzing blood cells and use thereof |
| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
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