CN106525552A - Serum sample diluent and detection kit - Google Patents
Serum sample diluent and detection kit Download PDFInfo
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- CN106525552A CN106525552A CN201611064271.9A CN201611064271A CN106525552A CN 106525552 A CN106525552 A CN 106525552A CN 201611064271 A CN201611064271 A CN 201611064271A CN 106525552 A CN106525552 A CN 106525552A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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Abstract
The invention provides a serum sample diluent and belongs to the technical field of biological sample detection. The serum sample diluent is prepared from components as follows: 0.3%-1.0% (v/v) of tetrabutyl ammonium hydroxide, 0.1%-0.5% (v/v) of triton X-100, 0.1%-1.2% (w/v) of EDTA (ethylene diamine tetraacetic acid), 0.1%-0.5% (w/v) ammonium nitrate and the balance of ultrapure water. The purity of a reagent is at least at the high-purity grade. The diluent and a method which are used for pretreatment of human serum microelement detection samples and are good in environment adaptability, high in measurement accuracy, simple to operate and suitable for large sample detection in the medical units are provided in the sample pretreatment link.
Description
Technical field
The present invention relates to biology sample detection technical field, specifically a kind of serum samples diluted liquid and detection kit.
Background technology
Trace element in life entity and chemistry, medicine and pharmacology, biology, environmentology, food science etc. have more close
The relation cut, in human body, content is little but plays an important role on some physiological functions are completed for trace element, various
Portion remains a kind of relative balance state to trace element in vivo, excessive or very few can cause disease.
The pre-treatment of sample is a process that is very time-consuming, loaded down with trivial details and being readily incorporated analysis evaluated error.With science
The progress of technology, analytical technology and analytical tool constantly develop, and sensitivity, precision and the automaticity to analyzing requires to get over
Come higher.For the complexity of the biological sample substrate such as human serum.The sample pre-treatments side of present human serum trace element detection
Method mainly has Wet method, Microwave Digestion and the method for directly diluting.Wet method is in heating with acid solution or alkali liquor
Under the conditions of destroy sample in Organic substance or reducing substanceses method, the method need consume it is substantial amounts of clear up reagent, to green
Bring unfavorable in colour circle border.Microwave Digestion is to drop sample at high temperature under high pressure thoroughly to clear up, and can avoid easily waving under closed environment
The loss of element is sent out, but microwave dissolver is costly, sample treatment is more time-consuming, is not suitable for the process of great amount of samples.Directly
Dilution method is the pre-treating method with diluent dilute sample, simple to operate, can process a large amount of samples in the short time, and serum is passed through
Directly can be detected as dilution dilution agent is latter, while greatly reducing the possibility polluted outside sample.But base in direct dilution method
Matter matching problem is the problem for needing concern.
It is contemplated that sample pre-treatments link provides a kind of good environmental adaptability, certainty of measurement is high, simple to operation, is adapted to
In diluent and the method for the human serum trace element detection sample pre-treatments of medical institutions' large sample detection.
The content of the invention
In view of this, it is the present invention is intended to provide a kind of good environmental adaptability, certainty of measurement are high, simple to operation, it is suitable for doctor
Treat the diluent of the human serum trace element detection sample pre-treatments of unit large sample detection.
For reaching above-mentioned purpose, the technical scheme is that what is be achieved in that：A kind of serum samples diluted liquid, including such as
Lower component：The positive TBAH of 0.3%-1.0%v/v, the triton x-100 of 0.1%-0.5%v/v, 0.1%-1.2%
The ammonium nitrate of EDTA, 0.1%-0.5%w/v of w/v, remaining is ultra-pure water.
Further, including following component：The positive TBAH of 0.5%-0.8%v/v, 0.2%-0.4%v/v
Triton x-100, the ammonium nitrate of EDTA, 0.2%-0.4%w/v of 0.5%-1.0%w/v, remaining is ultra-pure water.
Further, the positive TBAH of 0.6%v/v, the triton x-100 of 0.3%v/v, 0.8%w/v
The ammonium nitrate of EDTA, 0.3%w/v, remaining is ultra-pure water.
Further, diluent electrical conductivity at room temperature is 1800-2000 μ S/cm.
Further, the pH of the diluent is 7.0-7.5.
Further, agents useful for same purity is at least high-purity level.
Present invention also offers the preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
Present invention also offers application of the above-mentioned serum samples diluted liquid as the diluent of detection Trace Elements In Serum.
Present invention also offers the method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using institute
Diluent is stated by the 1/10 of serum-dilution to original content, by simultaneous determination of multielement inductance on the blood serum sample after dilution after standing
Coupled Plasma-Mass Spectroscopy device is detected.
Present invention also offers the detection kit comprising above-mentioned serum samples diluted liquid.
Relative to prior art, the present invention has the advantage that：
(1) it is contemplated that sample pre-treatments link provides a kind of good environmental adaptability, certainty of measurement is high, simple to operation,
It is suitable for diluent and the method for the human serum trace element detection sample pre-treatments of medical institutions' large sample detection.
(2) serum composition is relative complex, viscosity is big, containing various albumen, polypeptide and inorganic salts ingredients etc., if directly
Sample introduction, may blocking pipeline.The diluent of the present invention carries out a certain degree of dilution to serum, guarantees accurately inspection as far as possible
The micronutrient levelss surveyed in serum, and each component existence in serum is not affected, reach matrix matching and stablize in serum
The dual purpose of trace element.
The function of each component in the present invention：
Positive TBAH：Alkali organic solvent, provides carbon source for blood serum sample detection；
Triton x-100：Surfactant, increases dissolubility and the dispersibility of adipose membrane, and stable albumen is not precipitated；
Ammonium nitrate：Except the Sodium Chloride in serum deprivation, chloride ion combined with ammonium ion generation ammonium chloride, nitrate ion with
Sodium ion generates sodium nitrate, generates two kinds of compounds compared with easily ionizable, so as to the salification effect being effectively reduced in detection process
Should；
EDTA (disodiumedetate)：As a kind of metal ion stabilizing agents, effectively can stablize in serum
Metal ion.
Specific embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment one
A kind of serum samples diluted liquid, including following component：The positive TBAH of 0.6%v/v, 0.3%v/v
The ammonium nitrate of triton x-100, EDTA, 0.3%w/v of 0.8%w/v, remaining is ultra-pure water, and agents useful for same purity is at least high-purity
Level, diluent electrical conductivity at room temperature are 1800 μ S/cm, and pH is 7.0.
The preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
The method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using the diluent by serum
The 1/10 of original content is diluted to, by simultaneous determination of multielement inductive coupling plasma mass spectrometry on the blood serum sample after dilution after standing
Device is detected.
Embodiment two
A kind of serum samples diluted liquid, including following component：The positive TBAH of 0.5%v/v, 0.2%v/v
The ammonium nitrate of triton x-100, EDTA, 0.4%w/v of 0.5%w/v, remaining is ultra-pure water, and agents useful for same purity is at least high-purity
Level, diluent electrical conductivity at room temperature are 1900 μ S/cm, and pH is 7.1.
The preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
The method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using the diluent by serum
The 1/10 of original content is diluted to, by simultaneous determination of multielement inductive coupling plasma mass spectrometry on the blood serum sample after dilution after standing
Device is detected.
Embodiment three
A kind of serum samples diluted liquid, including following component：The positive TBAH of 0.8%v/v, 0.4%v/v
The ammonium nitrate of triton x-100, EDTA, 0.2%w/v of 1.0%w/v, remaining is ultra-pure water, and agents useful for same purity is at least high-purity
Level, diluent electrical conductivity at room temperature are 1950 μ S/cm, and pH is 7.2.
The preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
The method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using the diluent by serum
The 1/10 of original content is diluted to, by simultaneous determination of multielement inductive coupling plasma mass spectrometry on the blood serum sample after dilution after standing
Device is detected.
Example IV
A kind of serum samples diluted liquid, including following component：The positive TBAH of 0.3%v/v, 0.5%v/v
The ammonium nitrate of triton x-100, EDTA, 0.5%w/v of 0.1%w/v, remaining is ultra-pure water, and agents useful for same purity is at least high-purity
Level, diluent electrical conductivity at room temperature are 2000 μ S/cm, and pH is 7.3.
The preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
The method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using the diluent by serum
The 1/10 of original content is diluted to, by simultaneous determination of multielement inductive coupling plasma mass spectrometry on the blood serum sample after dilution after standing
Device is detected.
Embodiment five
A kind of serum samples diluted liquid, including following component：The positive TBAH of 1.0%v/v, 0.1%v/v
The ammonium nitrate of triton x-100, EDTA, 0.1%w/v of 1.2%w/v, remaining is ultra-pure water, and agents useful for same purity is at least high-purity
Level, diluent electrical conductivity at room temperature are 1850 μ S/cm, and pH is 7.5.
The preparation method of above-mentioned serum samples diluted liquid, comprises the steps：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution respectively and ammonium nitrate is molten
Liquid；
(2) with pipet accurately pipette the positive TBAH of formula ratio, triton x-100, ammonium nitrate solution and
EDTA solution, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
The method detected to Trace Elements In Serum by above-mentioned serum samples diluted liquid：Using the diluent by serum
The 1/10 of original content is diluted to, by simultaneous determination of multielement inductive coupling plasma mass spectrometry on the blood serum sample after dilution after standing
Device is detected.
Comparative example
Detect that to trace element detection data is as shown in Table 1 and Table 2 with five kinds of different diluent.Wherein dilute
Agent 5 is the diluent selected in the embodiment of the present invention one.
Table 1 compares the recovery of standard addition of different diluent
2 different diluent pair of tableThe detected value of-Serum control level II
Note：Diluent 1：Nitric acid (HNO3)
Diluent 2：HNO3+ triton x-100
Diluent 3：Positive TBAH (Tetra-n-butylammonium hydroxide)
Diluent 4：Positive TBAH+triton x-100
Diluent 5：Positive TBAH+triton x-100+disodiumedetate (EDTA)+ammonium nitrate
(NH4NO3)
-Serum control level II：Standard reference materialss known to a kind of concentration
Conclusion：From Tables 1 and 2, using the testing result of diluent 5 closest to reference value, it is seen then that its detection knot
Fruit is optimal.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (10)
1. a kind of serum samples diluted liquid, it is characterised in that：Including following component：The positive tetrabutyl hydrogen-oxygen of 0.3%-1.0%v/v
Change ammonium, the triton x-100 of 0.1%-0.5%v/v, the ammonium nitrate of EDTA, 0.1%-0.5%w/v of 0.1%-1.2%w/v, its
It is remaining for ultra-pure water.
2. serum samples diluted liquid according to claim 1, it is characterised in that：Including following component：0.5%-0.8%v/
The positive TBAH of v, the triton x-100 of 0.2%-0.4%v/v, EDTA, 0.2%- of 0.5%-1.0%w/v
The ammonium nitrate of 0.4%w/v, remaining is ultra-pure water.
3. serum samples diluted liquid according to claim 1, it is characterised in that：The positive tetrabutylammonium hydroxide of 0.6%v/v
Ammonium, the triton x-100 of 0.3%v/v, the ammonium nitrate of EDTA, 0.3%w/v of 0.8%w/v, remaining is ultra-pure water.
4. a kind of serum samples diluted liquid according to claim 1, it is characterised in that：Diluent electricity at room temperature
Conductance is 1800-2000 μ S/cm.
5. a kind of serum samples diluted liquid according to claim 1, it is characterised in that：The pH of the diluent is 7.0-
7.5。
6. a kind of serum samples diluted liquid according to any one of claim 1-5, it is characterised in that：Agents useful for same purity is extremely
It is high-purity level less.
7. a kind of method of the serum samples diluted liquid prepared as described in any one of claim 1-5, it is characterised in that：
(1) solid EDTA and ammonium nitrate is weighed respectively, and be configured to certain density EDTA solution and ammonium nitrate solution respectively；
(2) positive TBAH, triton x-100, ammonium nitrate solution and the EDTA for accurately pipetting formula ratio with pipet is molten
Liquid, ultrasonic disperse after mix homogeneously, obtain final product diluent in ultra-pure water.
8. the serum samples diluted liquid as described in any one of claim 1-5 is used as the diluent of detection Trace Elements In Serum
Using.
9. a kind of side detected to Trace Elements In Serum by serum samples diluted liquid as described in any one of claim 1-5
Method, it is characterised in that：Using the diluent by the 1/10 of serum-dilution to original content, by the blood serum sample after dilution after standing
Upper simultaneous determination of multielement inductive coupling plasma mass spectrometry device is detected.
10. a kind of detection kit of the serum samples diluted liquid comprising described in any one of claim 1-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611064271.9A CN106525552A (en) | 2016-11-28 | 2016-11-28 | Serum sample diluent and detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611064271.9A CN106525552A (en) | 2016-11-28 | 2016-11-28 | Serum sample diluent and detection kit |
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| CN106525552A true CN106525552A (en) | 2017-03-22 |
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| CN201611064271.9A Pending CN106525552A (en) | 2016-11-28 | 2016-11-28 | Serum sample diluent and detection kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345620A (en) * | 2020-10-22 | 2021-02-09 | 青岛惠安康生物工程有限公司 | Method for quantitatively detecting serum elements by inductively coupled plasma mass spectrometry |
| CN112014567B (en) * | 2020-08-20 | 2023-11-17 | 金华科生物技术河北有限公司 | Kit for measuring hypersensitive C reaction protein |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112014567B (en) * | 2020-08-20 | 2023-11-17 | 金华科生物技术河北有限公司 | Kit for measuring hypersensitive C reaction protein |
| CN112345620A (en) * | 2020-10-22 | 2021-02-09 | 青岛惠安康生物工程有限公司 | Method for quantitatively detecting serum elements by inductively coupled plasma mass spectrometry |
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