CN104914102B - A kind of micro-fluidic chip and application thereof - Google Patents
A kind of micro-fluidic chip and application thereof Download PDFInfo
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Abstract
The invention provides a kind of micro-fluidic chip and application thereof, micro-fluidic chip includes the chip entirety of transparent material, chip integrally includes the first mixing pit and the second mixing pit, and the first mixing pit and the second mixing pit are connected by microchannel, and microchannel is provided with the 8th valve；First mixing pit connects the first sample introduction microchannel, the second sample introduction microchannel, the 3rd sample introduction microchannel and the first stock layout microchannel, first sample introduction microchannel is provided with the first valve, second sample introduction microchannel is provided with the second valve, 3rd sample introduction microchannel is provided with the 3rd valve, and the first stock layout microchannel is provided with the 4th valve；Second mixing pit connects the 4th sample introduction microchannel, the 5th sample introduction microchannel and the second stock layout microchannel, and the 4th sample introduction microchannel is provided with the 5th valve, and the 5th sample introduction microchannel is provided with the 6th valve, and the second stock layout microchannel is provided with the 7th valve.This micro-fluidic chip has good effect for colorimetric determination lactic acid, so as to disclose its prospect in colorimetric method application field.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, refer specifically to a kind of colorimetric determination that can be used for and formulate the micro-fluidic of material
Chip, it is particularly possible to for modifying thia Graphene/γ-Fe2O3Pass through colorimetric determination lactic acid after nano composite material.
Background technology
In recent years, the miniaturization of analytical equipment has become an important research direction, the hair of microfluidic chip technology
Exhibition thus also earn widespread respect.Micro-fluidic chip is built by liquid storage tank, micro- reaction on chip by Micrometer-Nanometer Processing Technology
The microflow path system that micro- function element such as room, microchannel is constituted, after loading biological sample and reaction solution, in compression pump or electric field
Microfluidic circuit is formed under effect, in a kind of or continuous various reaction is carried out on chip, is reached to sample high flux, low consumption, fast
The purpose of speed analysis.Microfluidic analysis chip on a chip due to that with high integration, can complete sampling, dilution, add
Various functions such as reagent, reaction, separation, detection, the micro-full analytical system that is otherwise known as (Micro total analysis
System, μ-TAS).After microfluidic chip technology is set up, by the development of last decade, as on our times most before
One of the sciemtifec and technical sphere on edge.At present, micro-fluidic chip is widely used in biomedical, high-throughput drug synthesis and screening, environment
The various fields such as monitoring, biological agent detection.
In carbon material, it is by sp that graphite is dilute2The ultra-thin two-dimension web frame that hydbridized carbon atoms are constituted, as one kind by individual layer
Carbon atom dense arrangement into bi-dimensional cellular lattice structure carbon material, the conductive energy of Graphene is good, specific surface area is big and
The advantages of electron transfer rate is fast.The elements such as N, B be easily doped to graphite it is dilute in, but compared with B and N, S doping be very
Particularly, the electronegativity difference that S atom radius is far longer than between C atoms, and C (2.55) and S (2.58) is too small, it is impossible in C-
Obvious electric charge transfer is provided in S compounds, therefore, chemical doping S to graphite it is dilute in seem extremely difficult.However, theoretical meter
Show, the dilute structure of graphite can be distorted after doping S atom, so that the performance of Graphene is lifted, so, in the dilute middle incorporation of graphite
S atom it is critical that.Chinese patent (CN201310080785.3) discloses a kind of side that sulfur doping is carried out to Graphene
Method：Graphene is provided, the Graphene is placed in chemical vapour deposition reaction chamber；The reaction chamber is entered using inert gas
Row ventilation and pump-down process；Sulfur doping is carried out to the Graphene in sulphur source gas is passed through at 500~1050 DEG C；In hydrogen and lazy
The reaction chamber is lowered the temperature in property gas atmosphere.The method operation is cumbersome, and the chemical vapour deposition technique pair for being used
Equipment requirement is high.Fe in nature2O3Polymorphic form mutation known to have two kinds, i.e. α-Fe2O3With γ-Fe2O3.The former is in nature
Under the conditions of stabilization, referred to as bloodstone；The latter is not so good as α-Fe under field conditions (factors)2O3Stabilization, in metastable condition, referred to as magnetic
Bloodstone.γ-Fe2O3Nano-particle enjoys green grass or young crops because of it in the extensive use in the fields such as magnetic, catalysis, air-sensitive, biomedicine
Look at, but its unstability again limits its application, therefore prepare the γ-Fe for being capable of long period stable existence2O3With important
Meaning.Magnetic γ-Fe are prepared in recent years2O3Method have a lot, such as sol-gel process (Chinese patent application
CN200410086479.1), the method such as sintering process (Chinese patent application CN200710036501.5), these methods are certain
Temperature lower calcination and obtain γ-Fe2O3.Removed comprising γ-Fe in the magnetic composite obtained by some preparations2O3Phase, also includes
A number of α-Fe2O3.In addition, these γ-Fe2O3Heat endurance it is poor, in 400 DEG C or so γ-Fe2O3Will change
It is α-Fe2O3。
At present, the method for quantitative determination content of material is broadly divided into two major classes：Bioassary method and physical chemistry are determined
Method.The principle of bioassary method is that the sensitive reaction based on some biological tissues to material concentration to be measured is realized.But it is biological
The sensitivity and specificity of determination method are poor.Thus, recent decades, the development of bioassay method does not have made marked progress.Physics
The principle of chemical assay has a lot, and specific assay method has various, and such as gas-chromatography is combined method with flame ionic detector
The matter of (GC-FID) and efficient liquid phase-electrochemical detector combination (HLPC-ED) method and liquid chromatogram-charged spray ion gun
Spectrometer is combined method (LC/ESI-MS/MS) etc..Traditional chromatography actually use in all exist complex operation, sample treatment it is time-consuming,
Sensitivity and specificity not enough, apparatus expensive the shortcomings of.Therefore, simple, quick, economic, effective new detecting method has been developed
As extremely urgent important subject.Colorimetric detection is an importance in analysis science field, and its key is exactly handle
Detecting event is transformed into color change.Colorimetric detection has very big advantage compared with traditional detection, especially in operability, economy
The aspect such as property and practicality.Colorimetric method can be by naked eyes Direct Recognition reaction solution color change, it is not necessary to expensive or multiple
Miscellaneous instrument, operation is very easy, therefore can be applied to field assay and care diagnostic.
Lactic acid is widely present in organism as a kind of important organic acid, is the important product of organism anaerobic glycometabolism
Thing.Lactic acid is the carbohydate metabolism material in musculature, after animal slaughtering meat low temperature maturation during Muscle glycogen by anaerobic
Glycolysis generates lactic acid, so as to cause the decline of pH.The accumulating amount of lactic acid is proportionate with the decline degree of pH.Lactic acid is largely generated
Can cause Chilled Meats water-retaining property and the different degrees of decline of tenderness, have substantial connection with PSE meat, DFD meat.Analysis is determined
Lactic acid is significant in medical treatment and food industry, and to the qualitatively and quantitatively analysis of lactic acid for fully understanding food
Property and further research lactic acid physiological function be very important.At present, the detection method of lactic acid mainly includes EDTA
Compleximetry, enzyme process, Enzyme Electrode, liquid chromatography etc..These traditional detection methods it is each have by oneself itself adapt to scope and
Precision, also all receives the influence of cost, but these methods intuitively can not be recognized with the naked eye.Colorimetric detection is analysis science neck
One importance in domain, its key is exactly that detecting event is transformed into color change.Colorimetric detection has compared with traditional detection
Very big advantage, especially at aspects such as operability, economy and practicality.Colorimetric method can be reacted by naked eyes Direct Recognition
Solution colour changes, it is not necessary to the instrument of expensive or complexity, operates very easy, therefore can be applied to field assay and immediately
Diagnosis.
For prior art, the present invention devises a kind of micro-fluidic chip and is applied to the detection field of material, should
Chip can be used for quick, sensitive, quantitative determination lactic acid；Further, the micro-fluidic chip is after increasing strong magnets, energy
Enough convenient ferromagnetic materials of modification, present invention utilizes a kind of graphene composite material thia Graphene/γ-Fe of stabilization2O3
As decorative material and modified on micro-fluidic chip, because γ-Fe2O3With ferromagnetism, therefore facilitate repairing for material
Decorations；It is further experimental studies have found that, modified thia Graphene/γ-Fe2O3Afterwards, the effect of more preferable detection lactic acid has been reached
Really.The micro-fluidic chip and experimental technique of present invention design, it is simple, quick, sensitive, effective, and cost is relatively low, and it is more important
Be that the chip can be reused；By the use of microflow control technique, medicine used is reduced in experimentation and is combined
The consumption of material, has saved resource.
The content of the invention
Can be used for the micro-fluidic chip of lactate detection it is an object of the invention to provide a kind of, the chip is in modification thia stone
Black alkene/γ-Fe2O3Can more Sensitive Detection lactic acid after nano composite material.
The present invention is achieved by the following technical solutions：
A kind of micro-fluidic chip, including transparent material chip entirety, the chip integrally includes the first mixing pit and the
Two mixing pits, the first mixing pit and the second mixing pit are connected by microchannel, and microchannel is provided with the 8th valve；
First mixing pit connects the first sample introduction microchannel, the second sample introduction microchannel, the 3rd sample introduction microchannel and the first stock layout
Microchannel, the first sample introduction microchannel is provided with the first valve, and the second sample introduction microchannel is provided with the second valve, and the 3rd sample introduction is micro- logical
Road is provided with the 3rd valve, and the first stock layout microchannel is provided with the 4th valve；
Second mixing pit connects the 4th sample introduction microchannel, the 5th sample introduction microchannel and the second stock layout microchannel, the 4th sample introduction
Microchannel is provided with the 5th valve, and the 5th sample introduction microchannel is provided with the 6th valve, and the second stock layout microchannel is provided with the 7th valve
Door.
In such scheme, the overall material of the chip is dimethyl silicone polymer, polymethyl methacrylate or ITO materials
Matter.
In such scheme, chip entirety lower surface and dismountable strong magnetic is arranged right below positioned at the first mixing pit
Iron.
In such scheme, first mixing pit and the second mixing pit are equipped with anti-spilled cover plate, and anti-spilled cover plate is poly-
Dimethyl siloxane, polymethyl methacrylate or ITO materials.
Described micro-fluidic chip is used for colorimetric determination lactic acid.
The micro-fluidic chip is used to modify thia Graphene/γ-Fe2O3Nano composite material is by colorimetric determination breast
Acid.
The micro-fluidic chip is used to modify thia Graphene/γ-Fe2O3Nano composite material is by colorimetric determination breast
The purposes of acid, application method comprises the following steps：
Step 1, the first valve is opened, other valves are closed, by pump by thia Graphene/γ-Fe2O3Nano combined material
The aqueous dispersions of material send into the first mixing pit, close the first valve, thia Graphene/γ-Fe2O3Nano composite material is by strength
After magnet is fixed on the first mixing pit bottom, the 4th valve is opened, discharged water by pump, close the 4th valve, dry first
Mixing pit；
Step 2, the second valve of opening, the first mixing pit is sent the phosphate buffer solution containing LO into by pump
Afterwards, the second valve is closed；The 3rd valve is opened, after the phosphate buffer solution containing lactic acid is sent into the first mixing pit by pump,
Close the 3rd valve, incubation reaction；
Step 3, the 8th valve of opening, the second mixing pit is delivered to by pump by the mixed liquor after incubation reaction in step 2, is closed
Close the 8th valve；
Step 4, the 5th valve of opening, the second mixing pit is sent hac buffer into by pump, closes the 5th valve；Beat
The 6th valve is opened, 3,3 ', 5 will be contained by pump, the alcoholic solution of 5 '-tetramethyl benzidine sends into the second mixing pit, close the
Six valves, incubation reaction；
Step 5, light irradiate the second mixing pit, scan absorbance curve, record experimental data；
Step 6, the 7th valve of opening, are discharged reacted mixed liquor by pump；
Step 7, clean and dry micro-fluidic chip.
The first mixing pit and the second mixing pit in chip entirety are connected by microchannel, and the microchannel is provided with valve
Door；First mixing pit is used for composite catalysis LO decomposing lactic acid and produces H2O2, the second mixing pit is used for mixed from first
Close the H that pond enters2O23,3 ', 5,5 '-tetramethyl benzidine of catalysis (TMB) and light irradiate to carry out the detection of absorbance；Core
Piece entirety lower surface and it is arranged right below dismountable strong magnets positioned at the first mixing pit, strong magnets are used for magnetic absorption
Thia Graphene/γ-Fe2O3Nano composite material, when the first mixing pit of cleaning, the strong magnets that can dismantle are in order to clear
Wash；First mixing pit connects three sample introduction microchannels and the first stock layout microchannel, wherein the first sample introduction microchannel is used for composite wood
Expect the sample introduction of dispersion liquid, the second sample introduction microchannel is used for the sample introduction of LO solution, and the 3rd sample introduction microchannel is used for lactic acid
The sample introduction of solution, the first stock layout microchannel is used to discharge the liquid in composite dispersion liquid；Second mixing pit connects two and enters
Sample microchannel and the second stock layout microchannel, wherein, the 4th sample introduction microchannel is used for the sample introduction of hac buffer (ABS) solution,
5th sample introduction microchannel is used for the sample introduction of TMB, and the second stock layout microchannel is used to discharge final reaction solution.On all microchannels
It is equipped with valve and connects pump, the pump of all sample introduction microchannel connections is equipped with micro syringe.
Micro-fluidic chip is placed on temperature-controlled cabinet, pump and micro syringe that sample introduction microchannel is equipped with, for multiple
Condensation material dispersion liquid, LO solution, lactic acid solution, TMB solution and ABS sample introductions.Micro-fluidic chip is placed on transmission inspection
Survey on support, transmissive support top is provided with light launching fiber probe, transmitting probe is detected by collimating mirror with micro-fluidic chip
Mouth upper surface is affixed.Support lower section is equipped with detection fiber, and this detection fiber is affixed by collimating mirror with chip lower surface.Additionally,
The accurate light source of launching fiber connection, detection fiber probe is connected with spectrometer, the probe and precision light source phase of light launching fiber
Connect.
Beneficial effects of the present invention are：
(1) micro-fluidic chip line clear designed by, simple and cost is relatively low, and the chip can be reused.
(2) micro-fluidic chip designed by may be conveniently used colorimetric determination lactic acid, and the micro-fluidic chip is by increasing
Plus after strong magnets, ferromagnetic material can be modified, present invention utilizes a kind of graphene composite material thia stone of stabilization
Black alkene/γ-Fe2O3As decorative material and modified on micro-fluidic chip, because γ-Fe2O3With ferromagnetism, therefore
Facilitate the modification of material, it is further experimental studies have found that, modified thia Graphene/γ-Fe2O3Afterwards, reached preferably
Detect the effect of lactic acid.It is excellent that micro-fluidic chip designed by the present invention has that easy to operate, sample treatment is simple, sensitivity is high
Point.
(3) by the use of microflow control technique, the consumption of medicine used and composite is reduced in experimentation, is saved
About resource.
Brief description of the drawings
Fig. 1 is overlooking the structure diagram of the invention；
Fig. 2 is abosrption spectrogram of the present invention for colorimetric determination lactic acid.
In figure：The mixing pits of 1- first；The mixing pits of 2- second；The first sample introductions of 3- microchannel；The second sample introductions of 4- microchannel；5-
Three sample introduction microchannels；The first stock layouts of 6- microchannel；The sample introduction microchannels of 7- the 4th；The sample introduction microchannels of 8- the 5th；The stock layouts of 9- second are micro-
Passage；10- microchannels；The valves of 11- first；The valves of 12- second；The valves of 13- the 3rd；The valves of 14- the 4th；The valves of 15- the 5th；
The valves of 16- the 6th；The valves of 17- the 7th；The valves of 18- the 8th；19- strong magnets
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings：
As shown in figure 1, a kind of micro-fluidic chip, including dimethyl silicone polymer material, polymethyl methacrylate material
Or the chip entirety of ITO materials, the chip integrally includes the first mixing pit 1 and the second mixing pit 2, the first mixing pit 1 and the
Two mixing pits 2 are connected by microchannel 10, and microchannel 10 is provided with the 8th valve 18；It is micro- that first mixing pit 1 connects the first sample introduction
Passage 3, the second sample introduction microchannel 4, the 3rd sample introduction microchannel 5 and the first stock layout microchannel 6, the first sample introduction microchannel 3 is provided with
First valve 11, the second sample introduction microchannel 4 is provided with the second valve 12, and the 3rd sample introduction microchannel 4 is provided with the 3rd valve 13, the
One stock layout microchannel 6 is provided with the 4th valve 14；Second mixing pit 2 connects the 4th sample introduction microchannel 7, the 5th sample introduction microchannel 8
With the second stock layout microchannel 9, the 4th sample introduction microchannel 7 is provided with the 5th valve 15, and the 5th sample introduction microchannel 8 is provided with the 6th valve
Door 16, the second stock layout microchannel 9 is provided with the 7th valve 17；Chip entirety lower surface and positioned at the first mixing pit 1 just under
Side is provided with strong magnets 19, and strong magnets 19 are used for magnetic absorption thia Graphene/γ-Fe2O3Nano composite material；Described
One mixing pit 1 and the second mixing pit 2 are equipped with anti-spilled cover plate, and anti-spilled cover plate is dimethyl silicone polymer, polymethyl
Sour methyl esters or ITO materials.
Here is specific embodiment of the present invention for colorimetric determination lactic acid：
Embodiment 1
Step 1, open the first valve (11), close other valves, by pump by the thia Graphene of 200 μ L 2mg/mL/
γ-Fe2O3The aqueous dispersions the first mixing pit of feeding (1) of nano composite material, the first valve of closing (11), thia Graphene/
γ-Fe2O3After nano composite material is fixed on the first mixing pit (1) bottom by strong magnets (19), the 4th valve (14) is opened,
Water is discharged by pump, closes the 4th valve (14), dry the first mixing pit (1)；
Step 2, the second valve of opening (12), close other valves, and 100 μ L are contained into 0.5 unit lactic acid by pump aoxidizes
After the phosphate buffer solution (10mM, pH=7.4) of enzyme sends into the first mixing pit (1), the second valve (12) is closed；Open the 3rd valve
100 μ L are contained 10 μm of phosphate buffer solutions of the lactic acid of ol (10mM, pH=7.4) and send into the first mixing by door (13) by pump
Behind pond (1), the 3rd valve (13), incubation reaction are closed；
Step 3, the 8th valve (18) of opening, the second mixing is delivered to by pump by the mixed liquor after incubation reaction in step 2
Pond (2), closes the 8th valve (18)；
Step 4, the 5th valve (15) is opened, 200 μ L 0.2M hac buffers (pH=4.0) are sent into the by pump
Two mixing pits (2), close the 5th valve (15)；The 6th valve (16) is opened, 100 μ L are contained by 0.5mg 3 by pump, 3 ', 5,
The alcoholic solution of 5 '-tetramethyl benzidine sends into the second mixing pit (2), closes the 6th valve (16), incubation reaction；
Step 5, light irradiate the second mixing pit (2), and absorbance curve is scanned at λ=652nm, record experimental data；
Step 6, the 7th valve (17) of opening, are discharged reacted mixed liquor by pump；
Step 7, clean and dry micro-fluidic chip.
Embodiment 2
Step 1, the second valve of opening (12), the phosphoric acid buffer that 100 μ L are contained into 0.5 unit LO by pump are molten
After liquid (10mM, pH=7.4) sends into the first mixing pit (1), the second valve (12) is closed；The 3rd valve (13) is opened, by pump
100 μ L are contained after 10 μm of phosphate buffer solutions of the lactic acid of ol (10mM, pH=7.4) send into the first mixing pit (1), the is closed
Three valves (13), incubation reaction；
Step 2, the 8th valve (18) of opening, the second mixing is delivered to by pump by the mixed liquor after incubation reaction in step 1
Pond (2), closes the 8th valve (18)；
Step 3, the 5th valve (15) is opened, 200 μ L 0.2M hac buffers (pH=4.0) are sent into the by pump
Two mixing pits (2), close the 5th valve (15)；The 6th valve (16) is opened, 100 μ L are contained by 0.5mg 3 by pump, 3 ', 5,
The alcoholic solution of 5 '-tetramethyl benzidine sends into the second mixing pit (2), closes the 6th valve (16), incubation reaction；
Step 4, light irradiate the second mixing pit (2), and absorbance curve is scanned at λ=652nm, record experimental data；
Step 5, the 7th valve (17) of opening, are discharged reacted mixed liquor by pump；
Step 6, clean and dry micro-fluidic chip.
A curves are the scanning result of embodiment 1 in Fig. 2, and b curves are the scanning result of embodiment 2, it can be seen that this hair
Bright micro-fluidic chip may be advantageously employed in colorimetric determination lactic acid, and modify thia Graphene/γ-Fe2O3Afterwards, extinction
Degree is significantly increased, and illustrates that the micro-fluidic chip has effect well in colorimetric determination lactic acid, so as to disclose it in colorimetric
The prospect of method application field.
The above is only the preferred embodiments of the present invention, is not intended to limit embodiment of the present invention, and this area is general
Logical technical staff's central scope of the invention and spirit, can very easily carry out corresponding flexible or modification, certainly,
These modifications made according to present invention spirit, should all be included within scope of the present invention.
Claims (5)
1. a kind of purposes of micro-fluidic chip, it is characterised in that the micro-fluidic chip be used to modifying thia Graphene/γ-
Fe2O3Nano composite material passes through colorimetric determination lactic acid；
The micro-fluidic chip includes the chip entirety of transparent material, and the chip integrally includes the first mixing pit (1) and second
Mixing pit (2), the first mixing pit (1) and the second mixing pit (2) are connected by microchannel (10), and microchannel (10) are provided with the 8th
Valve (18)；
First mixing pit (1) connect the first sample introduction microchannel (3), the second sample introduction microchannel (4), the 3rd sample introduction microchannel (5) and
First stock layout microchannel (6), the first sample introduction microchannel (3) is provided with the first valve (11), and the second sample introduction microchannel (4) is provided with
Second valve (12), the 3rd sample introduction microchannel (5) is provided with the 3rd valve (13), and the first stock layout microchannel (6) is provided with the 4th
Valve (14)；
Second mixing pit (2) connects the 4th sample introduction microchannel (7), the 5th sample introduction microchannel (8) and the second stock layout microchannel (9),
4th sample introduction microchannel (7) is provided with the 5th valve (15), and the 5th sample introduction microchannel (8) is provided with the 6th valve (16), second
Stock layout microchannel (9) is provided with the 7th valve (17).
2. the purposes of a kind of micro-fluidic chip according to claim 1, it is characterised in that the overall material of the chip is
Dimethyl silicone polymer, polymethyl methacrylate or ITO materials.
3. the purposes of a kind of micro-fluidic chip according to claim 1, it is characterised in that the chip entirety lower surface and
Dismountable strong magnets (19) are arranged right below positioned at the first mixing pit (1).
4. the purposes of a kind of micro-fluidic chip according to claim 1, it is characterised in that first mixing pit (1) and
Second mixing pit (2) is equipped with anti-spilled cover plate, anti-spilled cover plate be dimethyl silicone polymer, polymethyl methacrylate or
ITO materials.
5. the purposes of a kind of micro-fluidic chip according to claim 1, it is characterised in that application method includes following step
Suddenly：
Step 1, the first valve (11) is opened, other valves are closed, by pump by thia Graphene/γ-Fe2O3Nano combined material
The aqueous dispersions of material send into the first mixing pit (1), close the first valve (11), thia Graphene/γ-Fe2O3Nano composite material
After being fixed on the first mixing pit (1) bottom by strong magnets (19), the 4th valve (14) is opened, discharged water by pump, closed
4th valve (14), dries the first mixing pit (1)；
Step 2, the second valve of opening (12), the first mixing pit is sent the phosphate buffer solution containing LO into by pump
(1) after, the second valve (12) is closed；The 3rd valve (13) is opened, the phosphate buffer solution containing lactic acid is sent into the by pump
After one mixing pit (1), the 3rd valve (13), incubation reaction are closed；
Step 3, the 8th valve (18) of opening, the second mixing pit is delivered to by pump by the mixed liquor after incubation reaction in step 2
(2) the 8th valve (18), is closed；
Step 4, the 5th valve (15) of opening, the second mixing pit (2) is sent hac buffer into by pump, closes the 5th valve
(15)；The 6th valve (16) is opened, 3,3 ', 5 will be contained by pump, the alcoholic solution feeding second of 5 '-tetramethyl benzidine is mixed
Close pond (2), close the 6th valve (16), incubation reaction；
Step 5, light irradiate the second mixing pit (2), scan absorbance curve, record experimental data；
Step 6, the 7th valve (17) of opening, are discharged reacted mixed liquor by pump；
Step 7, clean and dry micro-fluidic chip.
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| CN107051599A (en) * | 2017-05-15 | 2017-08-18 | 深圳先进技术研究院 | Micro-fluidic chip and micro-fluidic chip control method |
| WO2019080704A1 (en) * | 2017-10-25 | 2019-05-02 | 深圳华大生命科学研究院 | Microfluidic chip for nucleic acid synthesis |
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| CN102759621B (en) * | 2011-04-26 | 2016-02-24 | 复旦大学 | Based on the method for the high-flux rapid malaria serum detection of micro-fluidic chip |
| CN103983794B (en) * | 2013-02-12 | 2016-06-15 | 李木 | A kind of micro-fluidic chip and a kind of microfluidic methods |
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