CN107976354A - A kind of reagent for hemocyte analyzers - Google Patents
A kind of reagent for hemocyte analyzers Download PDFInfo
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- CN107976354A CN107976354A CN201711170152.6A CN201711170152A CN107976354A CN 107976354 A CN107976354 A CN 107976354A CN 201711170152 A CN201711170152 A CN 201711170152A CN 107976354 A CN107976354 A CN 107976354A
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- CN
- China
- Prior art keywords
- reagent
- hemolytic agents
- deionized water
- hemocyte analyzers
- leoii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 79
- 210000003677 hemocyte Anatomy 0.000 title claims abstract description 27
- 229940000351 hemocyte Drugs 0.000 title claims abstract description 27
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 41
- 239000000243 solution Substances 0.000 claims abstract description 33
- 238000004140 cleaning Methods 0.000 claims abstract description 25
- 238000010790 dilution Methods 0.000 claims abstract description 23
- 239000012895 dilution Substances 0.000 claims abstract description 23
- 238000004043 dyeing Methods 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 32
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000008367 deionised water Substances 0.000 claims description 23
- 229910021641 deionized water Inorganic materials 0.000 claims description 23
- 230000003204 osmotic effect Effects 0.000 claims description 18
- 239000011780 sodium chloride Substances 0.000 claims description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 14
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 13
- 235000011152 sodium sulphate Nutrition 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 11
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 10
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 10
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 5
- 239000005711 Benzoic acid Substances 0.000 claims description 5
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 5
- 235000010233 benzoic acid Nutrition 0.000 claims description 5
- WWAABJGNHFGXSJ-UHFFFAOYSA-N chlorophenol red Chemical compound C1=C(Cl)C(O)=CC=C1C1(C=2C=C(Cl)C(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 WWAABJGNHFGXSJ-UHFFFAOYSA-N 0.000 claims description 5
- 235000019253 formic acid Nutrition 0.000 claims description 5
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- WOWHHFRSBJGXCM-UHFFFAOYSA-M cetyltrimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)C WOWHHFRSBJGXCM-UHFFFAOYSA-M 0.000 claims description 4
- HXWGXXDEYMNGCT-UHFFFAOYSA-M decyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCC[N+](C)(C)C HXWGXXDEYMNGCT-UHFFFAOYSA-M 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 4
- 229960004889 salicylic acid Drugs 0.000 claims description 4
- CEYYIKYYFSTQRU-UHFFFAOYSA-M trimethyl(tetradecyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)C CEYYIKYYFSTQRU-UHFFFAOYSA-M 0.000 claims description 4
- 235000002639 sodium chloride Nutrition 0.000 claims description 2
- 238000012417 linear regression Methods 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 30
- 239000000523 sample Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 9
- 210000000601 blood cell Anatomy 0.000 description 8
- 210000003743 erythrocyte Anatomy 0.000 description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 229910052708 sodium Inorganic materials 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 239000000460 chlorine Substances 0.000 description 5
- 229910052801 chlorine Inorganic materials 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 3
- 108010054147 Hemoglobins Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 238000002242 deionisation method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000001044 red dye Substances 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 240000000203 Salix gracilistyla Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- AJXBTRZGLDTSST-UHFFFAOYSA-N amino 2-methylprop-2-enoate Chemical compound CC(=C)C(=O)ON AJXBTRZGLDTSST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention discloses a kind of reagent for hemocyte analyzers, it includes dilution, LEOI hemolytic agents, LEOII dyeing liquors, LH hemolytic agents, cleaning solution.The reagent for hemocyte analyzers of the present invention can step the replacement reagent of auspicious 5390 original-pack reagents of BC as the classification cytoanalyze of the prior art five, the problem of its is of high cost, delivery cycle is long can be efficiently solved, and with genuine reagent linear regression coefficient R up to more than 0.9900, system has preferable correlation；The raw material sources of the present invention are extensive, are easy to get, and cheap, nontoxic, environmental pollution is few；The simple production process of product of the present invention, is easy to duplication of production, stability is good, long shelf-life.
Description
Technical field
The present invention relates to extracorporeal diagnostic instrument reagent, and in particular to a kind of reagent for hemocyte analyzers.
Background technology
Mindray is the producer of the domestic classification cellanalyzer of production five of having the ability earliest, and wherein BC-5390 is one
Money is classified with binary channels leucocyte five and the Automatic Blood Cell Analyzer of abnormal cell screening function, using semiconductor laser
Flow cytometer showed technology, advanced cell constant temperature specific stain method.Precision is high, and residual contamination rate is low, and the range of linearity is wide, accurate
True property is good, can meet the job requirement of most clinical labororatories, have a extensive future.Can use venous whole, micro whole blood,
Three kinds of detection patterns of pre-dilution of blood are detected, because its peripheral blood pattern blood using amount is few, it is only necessary to 20 μ l blood, as current woman
The indispensable instrument of daughter child's hospital laboratory.
But at present step auspicious BC-5390 cellanalyzers original package reagent consumption it is big, and the cost of reagent and
It is expensive, cause medical treatment cost height.
The content of the invention
In view of the problems of the existing technology, the present invention provides a kind of reagent for hemocyte analyzers, particularly step auspicious
BC-5390 cellanalyzer replacement reagents.
The present invention uses following technical scheme：
A kind of reagent for hemocyte analyzers, it include dilution, LEOI hemolytic agents, LEOII dyeing liquors, LH hemolytic agents,
Cleaning solution；
The dilution include 3.5~8.0g/L of sodium chloride, 3.5~8.0g/L of sodium sulphate, disodium hydrogen phosphate 2.0~
5.0g/L, 1.0~3.0g/L of citric acid, 0.2~1.0g/L of EDTA-2Na, 0.3~1.0g/L of preservative；
The LEOI hemolytic agents include 2.0~5.0g/L of decyl trimethyl ammonium chloride, 2.0~8.0g/L of benzoic acid, chlorine
Change 2.0~7.0g/L of sodium, 2~6.0g/L of sodium sulphate；
The LEOII dyeing liquors include 1.0~4.0g/L of tetradecyl trimethyl ammonium chloride, 1.0~3.0g/L of acetic acid, chlorine
Change 5.0~10.0g/L of sodium, 4.0~8.0g/L of sodium sulphate, 5~7mg/L of chlorophenol red；
The LH hemolytic agents include 8.0~20.0g/L of hexadecyltrimethylammonium chloride, 2.0~5.0g/L of formic acid, bigcatkin willow
0.5~4.0g/L of acid, 10.0~20.0g/L of sodium chloride；
The cleaning solution includes 0.5~2.0g/L of TritionX-100, wherein liquor natrii hypochloritis, liquor natrii hypochloritis
Effective chlorine ＞ 5%.
Further, the pH value of the dilution is 6.5~7.2, and the electrical conductivity of the dilution is 15.0~19.0ms/
Cm, the osmotic pressure of the dilution is 260~330mOsm/kg.
Further, the disodium hydrogen phosphate weighed, citric acid, sodium chloride, sulfuric acid are sequentially added in 1000mL beakers
Sodium, EDTA-2Na, add suitable deionized water, and heating stirring is cooled to less than 40 DEG C, addition has claimed after it is completely dissolved
Good preservative, stirs evenly, and with the volume of deionized water constant volume to 1L, measures the pH value, electrical conductivity, osmotic pressure of solution, mistake
Up to the dilution after filter.
Further, the pH value of the LEOI hemolytic agents is 4.0~5.0, and the electrical conductivity of the LEOI hemolytic agents is 10.0
~16.0ms/cm, the osmotic pressure of the LEOI hemolytic agents is 330~380mOsm/kg.
Further, the decyl trimethyl ammonium chloride weighed, benzoic acid, chlorination are sequentially added in 1000mL beakers
Suitable ionized water is removed in sodium, sodium sulphate, addition, and heating stirring is after it is completely dissolved, with the volume of deionized water constant volume to 1L,
Stir evenly, measure the pH value, electrical conductivity, osmotic pressure of solution, up to the LEOI hemolytic agents after filtering.
Further, the pH value of the LEOII dyeing liquors is 9.50~11.0, and the electrical conductivity of the LEOII dyeing liquors is
21.0~25.0ms/cm, the osmotic pressure of the LEOII dyeing liquors is 450~500mOsm/kg.
Further, the tetradecyl trimethyl ammonium chloride weighed, sodium chloride, sulphur are sequentially added in 1000mL beakers
Sour sodium, adds suitable deionized water, and heating stirring is completely dissolved it, and acetic acid is added after cooling down, is added after stirring and dissolving
Chlorophenol red dye, stirring and dissolving, with the volume of deionized water constant volume to 1L, stirs evenly, measure the pH value of solution, electrical conductivity,
Osmotic pressure, up to the LEOII dyeing liquors after filtering.
Further, the pH value of the LH hemolytic agents is 2.0~3.0, the electrical conductivity of the LH hemolytic agents for 35.0~
40.0ms/cm, the osmotic pressure of the LH hemolytic agents is 600~700mOsm/kg.
Further, the hexadecyltrimethylammonium chloride weighed, salicylic acid, chlorine are sequentially added in 1000mL beakers
To change sodium, add suitable deionized water, heating stirring is completely dissolved it, is cooled to less than 40 DEG C, adds the formic acid weighed up,
Stirring, with the volume of deionized water constant volume to 1L, stirs evenly, measures the pH value, electrical conductivity, osmotic pressure of solution, after filtering i.e.
Obtain the LH hemolytic agents.
Further, the pH value of the cleaning solution is 10.0~12.0, the electrical conductivity of the cleaning solution for 25.0~
30.0ms/cm。
Further, the TritionX-100 weighed is sequentially added in 1000mL beakers, adds suitable deionization
Water, heating stirring are completely dissolved it, and the liquor natrii hypochloritis weighed up is added after cooling, and stirring, adds remaining deionization
Water constant volume stirs evenly to the volume of 1L, measures pH value, the electrical conductivity of solution, up to cleaning solution or probe cleaning solution after filtering.
The present invention have developed a whole set of and step auspicious BC-5390 cellanalyzers replacement reagent.
The dilution is that blood cell analysis with dilution, the LEOI hemolytic agents is blood cell analysis hemolytic agent, institute
It is that blood cell analysis with dyeing liquor, the LH hemolytic agents is blood cell analysis hemolytic agent, the cleaning solution to state LEOII dyeing liquors
For blood cell analysis cleaning solution or probe cleaning solution.
Current advanced five sorting technique of haemocyte, it is thin to employ Coulter principle, colorimetric method and semiconductor laser streaming
Born of the same parents' technology, can detect red blood cell, hemoglobin, blood platelet, leucocyte five classify, the parameter such as mean corpuscular volume (MCV), pass through
The number of detection human body haemocyte judges various diseases, monitors physical condition in time, reduces disease incidence probability or control
The state of an illness processed.
Need to be used together with matched reagent during the classification Blood cell analyzer detection blood cell numbers of BC-5390 five, it is supporting
Reagent major class has generally comprised dilution, hemolytic agent, dyeing liquor, cleaning solution.The main function of dilution provides one and blood plasma
The environment of identical osmotic pressure, electrical conductivity, LEOI hemolytic agents are lysed erythrocytes, keep leucocyte integrality, and leucocyte is carried out
Classification and counting, LEOII dyeing liquors carry out chemical staining to cell, to observe；LH hemolytic agents are lysed erythrocytes, are discharged
Hemoglobin, forms stable haemoglobin dervative with chelating agent, carries out the measure of hemoglobin concentration；The instrument is not equipped with
Cleaning solution, serves as cleaning solution, main function is Cleaning pipes, is mutually polluted between sample with dilution；Probe cleaning solution be exactly
Cleaning when shutdown or maintenance to instrument pipeline, specimen needle, avoids line clogging.
Beneficial effects of the present invention：
(1) product of the invention can step the original-pack reagents of auspicious BC-5390 as the classification cytoanalyze of the prior art five
Replacement reagent, can efficiently solve the problem of of high cost, delivery cycle is long；
(2) matched reagent accuracy of the invention it is high, it is reproducible, reached with genuine reagent linear regression coefficient R
More than 0.9900, system has preferable correlation；
(3) raw material sources of the invention are extensive, are easy to get, and cheap, nontoxic, environmental pollution is few；
(4) simple production process of product of the present invention, is easy to duplication of production, stability is good, long shelf-life；
(5) price of the invention can effectively reduce medical treatment cost, be worth further less than original-pack reagent up to 30% or so
Promote.
Brief description of the drawings
Fig. 1 is that the test result for the WBC projects that reagent made from the embodiment of the present invention 1 is surveyed respectively with stepping auspicious reagent contrasts
Correlation linear graph；
Fig. 2 is that the test result for the RBC projects that reagent made from the embodiment of the present invention 1 is surveyed respectively with stepping auspicious reagent contrasts
Correlation linear graph；
Fig. 3 is that the test result for the HGB projects that reagent made from the embodiment of the present invention 1 is surveyed respectively with stepping auspicious reagent contrasts
Correlation linear graph；
Fig. 4 is that the test result for the MCV projects that reagent made from the embodiment of the present invention 1 is surveyed respectively with stepping auspicious reagent contrasts
Correlation linear graph；
Fig. 5 is that the test result for the PLT projects that reagent made from the embodiment of the present invention 1 is surveyed respectively with stepping auspicious reagent contrasts
Correlation linear graph.
Embodiment
In order to preferably explain the present invention, it is described further in conjunction with specific examples below, but the present invention is unlimited
In specific embodiment.
Instrument：Auspicious BC-5390 cellanalyzers advanced in years, 1；
Step auspicious genuine reagent：M-53D dilutions, M-5LEO (I) hemolytic agent, M-5LEO (II) hemolytic agent, M-53LH haemolysis
Agent, M-53 probe cleaning solutions；
Reagent of the present invention：Dilution, LEOI hemolytic agents, LEOII dyeing liquors, LH hemolytic agents, cleaning solution.
Embodiment 1
BC-5390 cellanalyzers replacement reagent of the present invention
(1) formula of dilution of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the phosphoric acid hydrogen two weighed is sequentially added in 1000mL beakers
Sodium, citric acid, sodium chloride, sodium sulphate, EDTA-2Na, add suitable deionized water, and heating stirring is completely dissolved it.Material
After being completely dissolved, less than 40 DEG C are cooled to, the preservative weighed up is added, stirs evenly, the volume of constant volume to 1L, measures solution
PH value be 6.94, electrical conductivity 16.10ms/cm, osmotic pressure 285mOsm/kg, up to dilution after filtering.
(2) formula of LEOI hemolytic agents of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the ten alkyl front threes weighed are sequentially added in 1000mL beakers
Ammonium chloride, benzoic acid, sodium chloride, sodium sulphate, add suitable deionized water, and heating stirring is completely dissolved it, add surplus
Remaining deionized water, the volume of constant volume to 1L, stirs evenly, and the pH value for measuring solution is 4.20, electrical conductivity 14.10ms/cm,
Osmotic pressure is 360mOsm/kg, up to LEOI hemolytic agents after filtering.
(3) formula of LEOII dyeing liquors of the present invention：
Required material is accurately weighed by above-mentioned formula ratio, the myristyl three weighed is sequentially added in 1000mL beakers
Ammonio methacrylate, sodium chloride, sodium sulphate, add suitable deionized water, and heating stirring is completely dissolved it, is added after cooling down
Acetic acid, chlorophenol red dye is added after stirring and dissolving, stirring and dissolving, adds remaining deionized water, the volume of constant volume to 1L, is stirred
Uniformly, the pH value for measuring solution is 10.50, electrical conductivity 22.5ms/cm, osmotic pressure 480mOsm/kg, after filtering to obtain the final product
LEOII dyeing liquors.
(4) formula of LH hemolytic agents：
Required material is accurately weighed by above-mentioned formula ratio, the ten alkyl front threes weighed are sequentially added in 1000mL beakers
Ammonium chloride, salicylic acid, sodium chloride, add suitable deionized water, and heating stirring is completely dissolved it, be cooled to 40 DEG C with
Under, the formic acid weighed up is added, stirring, the volume of constant volume to 1L, stirs evenly, and the pH value for measuring solution is 2.20, electrical conductivity
For 36.10ms/cm, osmotic pressure 654mOsm/kg, up to LH hemolytic agents after filtering.
(5) formula of cleaning solution (probe cleaning solution) of the present invention：
TritionX-100 1.5g/L
Liquor natrii hypochloritis 700mL/L (liquor natrii hypochloritis's effective chlorine is 7.5%)
Required material is accurately weighed by above-mentioned formula ratio, the TritionX- weighed is sequentially added in 1000mL beakers
100, suitable deionized water is added, heating stirring is completely dissolved it.The liquor natrii hypochloritis weighed up is added after cooling, is stirred
The volume for being uniformly added into remaining deionized water constant volume to 1L is mixed, the pH value for measuring solution is 11.0, electrical conductivity 28.0ms/
Cm, osmotic pressure 485mOsm/kg, up to probe cleaning solution after filtering.
Experimental method and result：
By BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 with stepping auspicious original-pack BC M5 series
According to NCCLS files EP9-A2, (approval of American National Clinical Laboratory Standard committee paper refers to reagent for hemocyte analyzers
South-the second edition-carry out method with patient's sample compares bias evaluation) method is to same 120 (monstrosity containing WBC) fresh bloods
Sample test, obtains 120 groups of data, and data are listed in Table 1 below, to step auspicious original-pack BC M5 series reagent for hemocyte analyzers
Test result is abscissa, is vertical using BC-5390 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Coordinate, linear regression is done by Origin softwares, is obtained reagent made from the embodiment of the present invention 1 of Fig. 1 and is distinguished with stepping auspicious reagent
The test result contrast correlation linear graph for the WBC projects surveyed, the WBC project testing data in table 1 draw linear regression：Y
=1.02202X-0.12242, R=0.99532；
By BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 with stepping auspicious original-pack BC M5 series
According to NCCLS files EP9-A2, (approval of American National Clinical Laboratory Standard committee paper refers to reagent for hemocyte analyzers
South-the second edition-carry out method with patient's sample compares bias evaluation) method is to same 120 (monstrosity containing RBC) fresh bloods
Sample test, obtains 120 groups of data, and data are listed in Table 1 below, to step auspicious original-pack BC M5 series reagent for hemocyte analyzers
Test result is abscissa, is vertical using BC-5390 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Coordinate, linear regression is done by Origin softwares, is obtained reagent made from the embodiment of the present invention 1 of Fig. 2 and is distinguished with stepping auspicious reagent
The test result contrast correlation linear graph for the RBC projects surveyed, the RBC project testing data of table 1 draw linear regression：Y=
0.99935X-0.0086, R=0.99552；
By BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 with stepping auspicious original-pack BC M5 series
According to NCCLS files EP9-A2, (approval of American National Clinical Laboratory Standard committee paper refers to reagent for hemocyte analyzers
South-the second edition-carry out method with patient's sample compares bias evaluation) method is to same 120 (monstrosity containing HGB) fresh bloods
Sample test, obtains 120 groups of data, and data are listed in Table 1 below, to step auspicious original-pack BC M5 series reagent for hemocyte analyzers
Test result is abscissa, is vertical using BC-5390 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Coordinate, linear regression is done by Origin softwares, is obtained reagent made from the embodiment of the present invention 1 of Fig. 3 and is distinguished with stepping auspicious reagent
The test result contrast correlation linear graph for the HGB projects surveyed, the HGB project testing data of table 1 draw linear regression：Y=
0.99521X+0.51798, R=0.99549；
By BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 with stepping auspicious original-pack BC M5 series
According to NCCLS files EP9-A2, (approval of American National Clinical Laboratory Standard committee paper refers to reagent for hemocyte analyzers
South-the second edition-carry out method with patient's sample compares bias evaluation) method is to same 120 (monstrosity containing MCV) fresh bloods
Sample test, obtains 120 groups of data, and data are listed in Table 1 below, to step auspicious original-pack BC M5 series reagent for hemocyte analyzers
Test result is abscissa, is vertical using BC-5390 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Coordinate, linear regression is done by Origin softwares, is obtained reagent made from the embodiment of the present invention 1 of Fig. 4 and is distinguished with stepping auspicious reagent
The test result contrast correlation linear graph for the MCV projects surveyed, the MCV project testing data of table 1 draw linear regression：Y=
0.99105X+1.16955, R=0.98957；
By BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 with stepping auspicious original-pack BC M5 series
According to NCCLS files EP9-A2, (approval of American National Clinical Laboratory Standard committee paper refers to reagent for hemocyte analyzers
South-the second edition-carry out method with patient's sample compares bias evaluation) method is to same 120 (monstrosity containing PLT) fresh bloods
Sample test, obtains 120 groups of data, and data are listed in Table 1 below, to step auspicious original-pack BC M5 series reagent for hemocyte analyzers
Test result is abscissa, is vertical using BC-5390 cellanalyzers replacement reagent test result made from the embodiment of the present invention 1
Coordinate, linear regression is done by Origin softwares, is obtained reagent made from the embodiment of the present invention 1 of Fig. 5 and is distinguished with stepping auspicious reagent
The test result contrast correlation linear graph for the PLT projects surveyed, the PLT project testing data of table 1 draw linear regression:Y=
1.01557X-1.82887 R=0.99046；
Table 1：BC-5390 cellanalyzers replacement reagent made from the embodiment of the present invention 1 is with stepping auspicious original-pack BC M5 systems
(wherein (i) is advanced in years to the experiment raw data results for WBC, RBC, HGB, MCV, PLT project that row cellanalyzer reagent place surveys
The data of auspicious original-pack BC M-5 series of cell analyzer reagents, (I) are BC-5390 haemocytes made from the embodiment of the present invention 1 point
The data of analyzer replacement reagent).
The foregoing is merely the specific embodiment of the present invention, it is not intended to limit the scope of the invention, every utilization
The equivalent transformation that the present invention makees, is directly or indirectly used in other relevant technical fields, is similarly included in the present invention's
Among scope of patent protection.
Claims (10)
- A kind of 1. reagent for hemocyte analyzers, it is characterised in that it include dilution, LEOI hemolytic agents, LEOII dyeing liquors, LH hemolytic agents, cleaning solution；The dilution include 3.5~8.0g/L of sodium chloride, 3.5~8.0g/L of sodium sulphate, 2.0~5.0g/L of disodium hydrogen phosphate, 1.0~3.0g/L of citric acid, 0.2~1.0g/L of EDTA-2Na, 0.3~1.0g/L of preservative；The LEOI hemolytic agents include 2.0~5.0g/L of decyl trimethyl ammonium chloride, 2.0~8.0g/L of benzoic acid, sodium chloride 2.0~7.0g/L, 2~6.0g/L of sodium sulphate；The LEOII dyeing liquors include 1.0~4.0g/L of tetradecyl trimethyl ammonium chloride, 1.0~3.0g/L of acetic acid, sodium chloride 5.0~10.0g/L, 4.0~8.0g/L of sodium sulphate, 5~7mg/L of chlorophenol red；The LH hemolytic agents include 8.0~20.0g/L of hexadecyltrimethylammonium chloride, 2.0~5.0g/L of formic acid, salicylic acid 0.5~4.0g/L, 10.0~20.0g/L of sodium chloride；The cleaning solution includes 0.5~2.0g/L of TritionX-100, liquor natrii hypochloritis.
- 2. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the dilution is 6.5 ~7.2, the electrical conductivity of the dilution is 15.0~19.0ms/cm, and the osmotic pressure of the dilution is 260~330mOsm/ kg。
- 3. reagent for hemocyte analyzers according to claim 1 or 2, it is characterised in that title is sequentially added in beaker Disodium hydrogen phosphate, citric acid, sodium chloride, sodium sulphate, the EDTA-2Na taken, adds deionized water, and heating stirring treats that its is complete After dissolving, less than 40 DEG C are cooled to, the preservative weighed up is added, stirs evenly, with the volume of deionized water constant volume to 1L, mistake Up to the dilution after filter.
- 4. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the LEOI hemolytic agents is 4.0~5.0, the electrical conductivity of the LEOI hemolytic agents is 10.0~16.0ms/cm, and the osmotic pressure of the LEOI hemolytic agents is 330 ~380mOsm/kg.
- 5. the reagent for hemocyte analyzers according to claim 1 or 4, it is characterised in that title is sequentially added in beaker Decyl trimethyl ammonium chloride, benzoic acid, sodium chloride, the sodium sulphate taken, adds deionized water, and heating stirring treats that it is completely molten Xie Hou, with the volume of deionized water constant volume to 1L, stirs evenly, up to the LEOI hemolytic agents after filtering.
- 6. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the LEOII dyeing liquors For 9.50~11.0, the electrical conductivity of the LEOII dyeing liquors is 21.0~25.0ms/cm, the osmotic pressure of the LEOII dyeing liquors For 450~500mOsm/kg.
- 7. the reagent for hemocyte analyzers according to claim 1 or 6, it is characterised in that title is sequentially added in beaker Tetradecyl trimethyl ammonium chloride, sodium chloride, the sodium sulphate taken, adds deionized water, and heating stirring is completely dissolved it, so After add acetic acid, chlorophenol red is added after stirring and dissolving, stirring and dissolving, with the volume of deionized water constant volume to 1L, stirs evenly, mistake Up to the LEOII dyeing liquors after filter.
- 8. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the LH hemolytic agents is 2.0~3.0, the electrical conductivity of the LH hemolytic agents is 35.0~40.0ms/cm, the osmotic pressure of the LH hemolytic agents for 600~ 700mOsm/kg。
- 9. the reagent for hemocyte analyzers according to claim 1 or 8, it is characterised in that title is sequentially added in beaker Hexadecyltrimethylammonium chloride, salicylic acid, the sodium chloride taken, adds deionized water, and heating stirring is completely dissolved it, drops Temperature adds the formic acid weighed up to less than 40 DEG C, and stirring, with the volume of deionized water constant volume to 1L, stirs evenly, after filtering i.e. Obtain the LH hemolytic agents.
- 10. reagent for hemocyte analyzers according to claim 1, it is characterised in that the pH value of the cleaning solution is 10.0~12.0, the electrical conductivity of the cleaning solution is 25.0~30.0ms/cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655604A (en) * | 2018-12-27 | 2019-04-19 | 山东博科生物产业有限公司 | A set of universal three classification blood cell analysis reagent |
| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
| CN113267395A (en) * | 2021-07-05 | 2021-08-17 | 江苏荣盛嘉美生物试剂有限公司 | Reagent for urine visible component analyzer and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101281193A (en) * | 2008-06-04 | 2008-10-08 | 南昌百特生物高新技术有限公司 | Blood cell analyzer dilution as well as hemolytic agent |
| CN101819199A (en) * | 2010-05-08 | 2010-09-01 | 桂林市朗道诊断用品有限公司 | Reagent for hemocyte analyzers |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN103323581A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Hemocyte analyzer hemolytic agent |
| CN104698157A (en) * | 2015-02-13 | 2015-06-10 | 中山市创艺生化工程有限公司 | Agent for blood cell analyzer |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
| CN107144519A (en) * | 2017-05-24 | 2017-09-08 | 中山市滔略生物科技有限公司 | Cellanalyzer hemolytic agent and preparation method thereof |
-
2017
- 2017-11-22 CN CN201711170152.6A patent/CN107976354A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101281193A (en) * | 2008-06-04 | 2008-10-08 | 南昌百特生物高新技术有限公司 | Blood cell analyzer dilution as well as hemolytic agent |
| CN101819199A (en) * | 2010-05-08 | 2010-09-01 | 桂林市朗道诊断用品有限公司 | Reagent for hemocyte analyzers |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN103323581A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Hemocyte analyzer hemolytic agent |
| CN104698157A (en) * | 2015-02-13 | 2015-06-10 | 中山市创艺生化工程有限公司 | Agent for blood cell analyzer |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
| CN107144519A (en) * | 2017-05-24 | 2017-09-08 | 中山市滔略生物科技有限公司 | Cellanalyzer hemolytic agent and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655604A (en) * | 2018-12-27 | 2019-04-19 | 山东博科生物产业有限公司 | A set of universal three classification blood cell analysis reagent |
| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
| CN111521834B (en) * | 2020-03-20 | 2023-03-24 | 佛山市顺德区德维医疗科技有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
| CN113267395A (en) * | 2021-07-05 | 2021-08-17 | 江苏荣盛嘉美生物试剂有限公司 | Reagent for urine visible component analyzer and preparation method thereof |
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