CN103472034B - A kind of blood cell analysis chip, analyser and analytical approach - Google Patents
A kind of blood cell analysis chip, analyser and analytical approach Download PDFInfo
- Publication number
- CN103472034B CN103472034B CN201310372923.5A CN201310372923A CN103472034B CN 103472034 B CN103472034 B CN 103472034B CN 201310372923 A CN201310372923 A CN 201310372923A CN 103472034 B CN103472034 B CN 103472034B
- Authority
- CN
- China
- Prior art keywords
- storage tank
- liquid storage
- fluid passage
- blood cell
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 80
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 52
- 238000013459 approach Methods 0.000 title abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 95
- 210000004027 cell Anatomy 0.000 claims abstract description 83
- 210000003714 granulocyte Anatomy 0.000 claims abstract description 61
- 102000003992 Peroxidases Human genes 0.000 claims abstract description 60
- 108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 60
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 39
- 238000000034 method Methods 0.000 claims abstract description 34
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 31
- 102000001554 Hemoglobins Human genes 0.000 claims abstract description 26
- 108010054147 Hemoglobins Proteins 0.000 claims abstract description 26
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 19
- 238000010186 staining Methods 0.000 claims abstract description 14
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 238000002356 laser light scattering Methods 0.000 claims abstract description 10
- 238000004090 dissolution Methods 0.000 claims abstract description 8
- 238000000790 scattering method Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 126
- 238000003860 storage Methods 0.000 claims description 90
- 239000012530 fluid Substances 0.000 claims description 63
- 210000004369 blood Anatomy 0.000 claims description 43
- 239000008280 blood Substances 0.000 claims description 43
- 239000002699 waste material Substances 0.000 claims description 34
- 238000010790 dilution Methods 0.000 claims description 26
- 239000012895 dilution Substances 0.000 claims description 26
- 238000000429 assembly Methods 0.000 claims description 20
- 230000000712 assembly Effects 0.000 claims description 20
- 238000012545 processing Methods 0.000 claims description 19
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 18
- 230000010100 anticoagulation Effects 0.000 claims description 18
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 12
- 238000000149 argon plasma sintering Methods 0.000 claims description 11
- WONRDHPFOHAWOG-UHFFFAOYSA-N 2-chloronaphthalen-1-ol Chemical class C1=CC=C2C(O)=C(Cl)C=CC2=C1 WONRDHPFOHAWOG-UHFFFAOYSA-N 0.000 claims description 9
- 238000005516 engineering process Methods 0.000 claims description 9
- 238000005259 measurement Methods 0.000 claims description 9
- -1 oxonium ion Chemical class 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000006911 enzymatic reaction Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 238000000354 decomposition reaction Methods 0.000 claims description 4
- 230000003834 intracellular effect Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000005502 peroxidation Methods 0.000 claims description 4
- 239000002244 precipitate Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 229920002521 macromolecule Polymers 0.000 claims description 3
- 229910021421 monocrystalline silicon Inorganic materials 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 description 12
- 210000004698 lymphocyte Anatomy 0.000 description 10
- 210000001616 monocyte Anatomy 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 210000003979 eosinophil Anatomy 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 210000004493 neutrocyte Anatomy 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000009826 distribution Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000007689 inspection Methods 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- 238000009534 blood test Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 230000001744 histochemical effect Effects 0.000 description 2
- 239000000644 isotonic solution Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 210000001501 megacaryocyte Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000001167 myeloblast Anatomy 0.000 description 2
- 230000003448 neutrophilic effect Effects 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001894 hemadsorption Effects 0.000 description 1
- 210000005096 hematological system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The invention belongs to blood cell analysis technical field, particularly relate to a kind of blood cell analysis chip, analyser and analytical approach.Blood cell analysis chip of the present invention comprises: leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip, described leukocyte analysis chip comprises peroxidase detection chip and basophilic granulocyte detection chip, described peroxidase detection chip utilizes laser light scattering and peroxidase staining technique to carry out cell classification, described basophilic granulocyte detection chip adopts biological dissolution and electrical impedance method to detect basophilic granulocyte, described red blood cell, blood platelet or hemoglobin analysis chip adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin.Blood cell analysis chip of the present invention, analyser and analytical approach have that structure is simple, volume is little, cost is low, easy to operate, easy care, easily transport, chip mistake and the advantage such as discardable.<pb pnum="1" />
Description
Technical field
The invention belongs to blood cell analysis technical field, particularly relate to a kind of blood cell analysis chip, analysis and analytical approach.
Background technology
The information that blood cell analysis obtains can contribute to the disease diagnosed, antidiastole is relevant with hematological system, contribute to analysing patient's condition, observe the curative effect, judging prognosis, for prevent disease provides foundation, instruct clinical application and carry out clinic study, therefore haemocyte inspection (i.e. routine blood test) becomes first of three large routine inspections in clinical examination (routine blood test, routine urinalysis, just routine), and its clinical practice is also extensive.After nineteen fifty-three Mr. Ku Erte invention electrical impedance method blood-counter system, various Automatic blood cell analyzer is come out one after another, and blood cell analysis technology is developed rapidly.
At present for blood cell analysis, generally carried out under given conditions through specific people by commercially available cellanalyzer.Existing cellanalyzer is bulky, expensive, complicated operation, need special messenger to use and carry out periodic maintenance, the measurement reagent price supporting with it is also more expensive, generally be applicable to the more hospital inspection section office comparatively concentrated of test samples, for different medical units such as village health posts, often sample size is little, and sample disperses very much on time dimension, there is the situation of significant discomfort, therefore conventional inspection kind equipment shortcoming should be overcome, meet the demand of different medical unit to measuring means again, current urgent need develops portability, simple operation, the cellanalyzer of just-in-time of reporting the result, be applicable to Site Detection, emergency analysis, domestic. applications and primary care.
Summary of the invention
The invention provides a kind of blood cell analysis chip, analyser and analytical approach, be intended to solve that existing cellanalyzer is bulky, expensive, complicated operation, need special messenger to use and carry out the technical matters of periodic maintenance.
Technical scheme provided by the invention is: a kind of blood cell analysis chip, comprise: leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip, described leukocyte analysis chip comprises peroxidase detection chip and basophilic granulocyte detection chip, described peroxidase detection chip utilizes laser light scattering and peroxidase staining technique to carry out cell classification, described basophilic granulocyte detection chip adopts biological dissolution and electrical impedance method to detect basophilic granulocyte, described red blood cell, blood platelet or hemoglobin analysis chip adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin.
Technical scheme of the present invention also comprises: described peroxidase detection chip is provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies, described liquid storage tank is for storing detection reagent, described waste liquid pool is for storing the blood sample through detecting, described detection zone carries out laser beam irradiation for making the leucocyte of blood sample through detection zone, classified by light scattering and the different blood sample to detecting of cell size, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
Technical scheme of the present invention also comprises: described basophilic granulocyte detection chip is provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies, described liquid storage tank is used for storage of blood sample and dilution, described waste liquid pool is for storing the blood sample through detecting, the cell that described detection zone is used for blood sample adopts electrical impedance technology to detect according to cell size through detection zone, calculated the Effective Numerical of leucocyte bare nucleus and basophilic granulocyte by electronic impulse size and number, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
Technical scheme of the present invention also comprises: described red blood cell, blood platelet or haemoglobin detection chip are provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies, described liquid storage tank is used for storage of blood sample and dilution, described waste liquid pool is for storing the blood sample through detecting, described detection zone carries out laser beam irradiation for making the leucocyte of blood sample through detection zone, classified by light scattering and the different blood sample to detecting of cell size, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
Technical scheme of the present invention also comprises: described leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip adopt material to comprise quartz, glass, monocrystalline silicon or macromolecule polymeric material; Described liquid storage tank sample introduction adopts Micropump, electrokinetic injection, positive pressure to drive sample introduction, Ngatively pressurized sampling or electric osmose sample introduction various ways.
Another technical scheme provided by the invention is: a kind of cellanalyzer, comprise blood cell analysis chip as claimed in claim 1, detecting unit and signal processing system, described blood cell analysis chip, detecting unit is connected successively with signal processing system, described blood cell analysis chip is for carrying haemocyte, described detecting unit is used for applying laser beam to detection zone and irradiates haemocyte, described signal processing system applies steady current to upper and lower two ends, detection zone, detect each cell and produce the electronic impulse proportional with cell volume, according to the threshold value of setting, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical accurately, or described signal processing system adopts high angle scattered light to measure cell peroxidase content situation, endoerythrocytic hemoglobin concentration, content of hemoglobin mean value or measurement cell refractive index, described signal processing system adopts low angle scattered light to measure cell volume or size.
The another technical scheme that the present invention takes is: a kind of blood cell analysis method, comprising:
Step a: utilize laser light scattering and peroxidase staining technique to classify to cell;
Step b: adopt biological dissolution technology and electrical impedance method to detect basophilic granulocyte;
Step c: adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin.
Technical scheme of the present invention also comprises: described step a comprises: sample introduction is to liquid storage tank S1, R1, R2 respectively for anticoagulation, dilution, clean-out system and formaldehyde isotonic liquid, and whole blood carries out suitably dilution and the short time hatches; Mix and heat with hydrogen peroxide and four chloro-naphthols through liquid storage tank R5, R6 respectively, intracellular peroxidation thing enzyme decomposition of hydrogen peroxide produces oxonium ion, and oxonium ion makes four chloro-naphthols develop the color and precipitate and is positioned enzyme reaction position; Sheath fluid sample introduction is to liquid storage tank R7, and through the blood sample of chemical staining under the effect of sheath fluid stream, leucocyte is in line and successively by detection zone A1; Flow into waste liquid pool W1 through laser beam irradiation, classify according to light scattering and cell size difference.
Technical scheme of the present invention also comprises: described step b comprises: anticoagulation sample introduction is to liquid storage tank S2, special basophilic granulocyte dilution sample introduction is to liquid storage tank R3, quantitative anticoagulation and quantitative dilution flow out from respective liquid storage tank and mix, sheath fluid sample introduction is to liquid storage tank R8, treated blood sample is under the effect of quantitative sheath fluid stream, leucocyte is in line and successively by detection zone A2, A2 upper and lower two ends in detection zone apply steady current, detect through electrical impedance and flow into waste liquid pool W2, according to threshold value setting through signal processing system Treatment Analysis, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical accurately.
Technical scheme of the present invention also comprises: in described step c, described pair of multi-angle laser method detects to comprise and uses low angle scattered light and high angle scattered light, wherein, described low angle scattered light is for measuring erythrocyte volume, high angle scattered light measures each endoerythrocytic hemoglobin concentration and content of hemoglobin mean value, on platelet count is analyzed, high angle scattering optical power measurement cell refractive index, low angle scattering optical power measurement cell size.
Technical scheme tool of the present invention has the following advantages or beneficial effect: the blood cell analysis chip of the embodiment of the present invention, analyser and analytical approach carry out somatotype to haemocyte in anticoagulation, have that structure is simple, volume is little, cost is low, easy to operate, easy care, easily transport, chip mistake and the advantage such as discardable, meet the demand for development of analytical instrument microminiaturization, integrated and portability, be applicable to the uses such as hospital, clinic, community and individual family.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of the blood cell analysis chip of the embodiment of the present invention;
Accompanying drawing 2 is structural representations of the peroxidase detection chip of the blood cell analysis chip of the embodiment of the present invention;
Accompanying drawing 3 is structural representations of the basophilic granulocyte detection chip of the blood cell analysis chip of the embodiment of the present invention;
Accompanying drawing 4 is structural representations of the red blood cell of the blood cell analysis chip of the embodiment of the present invention, blood platelet or haemoglobin detection chip;
Accompanying drawing 5 is structural representations of the cellanalyzer of the embodiment of the present invention;
Accompanying drawing 6 is embody rule structural representations of the cellanalyzer of the embodiment of the present invention;
Accompanying drawing 7 is process flow diagrams of the blood cell analysis method of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Referring to Fig. 1, is the structural representation of the blood cell analysis chip of the embodiment of the present invention.The blood cell analysis chip of the embodiment of the present invention comprises leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip.
Wherein, leukocyte analysis chip comprises: peroxidase detection chip and basophilic granulocyte detection chip.Refer to Fig. 2, Fig. 2 is the structural representation of the peroxidase detection chip of the blood cell analysis chip of the embodiment of the present invention.Peroxidase detection chip is provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies.Liquid storage tank is for storing detection reagent, waste liquid pool is for storing the blood sample through detecting, detection zone carries out laser beam irradiation for making the leucocyte of blood sample through detection zone, different thus classify to the blood sample detected by light scattering and cell size, dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.In embodiments of the present invention, liquid storage tank arranges at least one, its can be used for storing cell cleaned and destroys detection reagent, make cell carry out the zymolytic detection reagent of superoxide and sheath fluid.Peroxidase detection chip utilizes laser light scattering and peroxidase staining technique to carry out cell classification, peroxidase detection chip carries out cell classification detailed process: anticoagulation, dilution, clean-out system and formaldehyde isotonic liquid difference sample introduction are to liquid storage tank S1, R1, R2, wherein, isotonic solution is in the various solution used in clinical or Physiological Experiment, what its osmotic pressure was equal with plasma osmolarity is called isotonic solution, whole blood carries out suitably dilution and the short time hatches, now cell is destroyed by clean-out system, leucocyte slurry endoenzyme is fixed, by liquid storage tank R5, R6 mixes with hydrogen peroxide and four chloro-naphthols and heats respectively, now intracellular peroxidation thing enzyme decomposition of hydrogen peroxide produces oxonium ion, oxonium ion makes four chloro-naphthols develop the color and precipitate and is positioned enzyme reaction position, sheath fluid sample introduction is to R7 liquid storage tank, through the blood sample of chemical staining under the effect of sheath fluid stream, leucocyte is in line and flows into waste liquid pool W1 by detection zone A1 through laser beam irradiation one by one, be classified due to light scattering and cell size difference, dosing assemblies C1 to C7 is used for making corresponding fluids in each liquid storage tank quantitative.Appears as negative reaction without black-and-blue particle in cell after dyeing, occur that the black particle of fine particle or sparse distribution is weak positive reaction, occur that the thick and intensive particle of black is strong positive reaction.All kinds of leucocyte is to the reaction of peroxidase: early stage myeloblast is negative, each stage that early young grain is later all contains peroxidase, and strengthen gradually along with its content of maturation of cell, eosinophil has the strongest peroxidase, neutrophil leucocyte contains stronger peroxidase, and basophilic granulocyte is not containing this enzyme; In monocyte system, except the early stage primitive stage, inmature monokaryon and monocyte there will be more weak peroxidase reaction; Lymphocyte, erythroneocytosis, megacaryocyte etc. are all peroxidase negative reaction.To sum up, in blood five kinds of leukocytic peroxidase activities put in order into: eosinophil > neutrophil leucocyte > monocyte, lymphocyte and basophilic granulocyte are all without peroxidase.Due to after enzyme reaction in intensity difference (feminine gender, the weak positive, strong positive) and cell volume difference in size, when cell passes through detection zone A1 through laser beam irradiation, produce the scattered light of varying strength, each cell produces histochemical staining result and light scattering result two signals.
At the scatter diagram that peroxidase detection chip produces, transverse axis represents the cell peroxidase content situation obtained 7 ° of laser light scattering absorptances, and peroxidase strong positive cell is positioned at right-hand member; The longitudinal axis is the cell volume size that low angle scattered light 2.8 ° is measured, and the expression scattered light signal being positioned at top is strong, and cell volume is large.Except basophilic granulocyte, the cell of each type is recorded in specific position according to their feature, eosinophil, neutrophil leucocyte, monocyte and lymphocyte can be distinguished in setting range, and calculate total white blood cells and classification in conjunction with basophilic granulocyte detection chip result.To sum up, cell is divided into five classes by peroxidase detection chip: the 1) eosinophil of the most strong positive of peroxidase; 2) neutrophilic segmented granulocyte of peroxidase strong positive; 3) comparatively large, the p+ monocyte of peroxidase of volume; 4) lymphocyte of small volume, peroxidase feminine gender; 5) volume is greater than lymphocyte and the maxicell that is unstained of peroxidase feminine gender, and this type of cell increases prompting naivety or original various types of cells may occur.
Referring to Fig. 3, is the structural representation of the basophilic granulocyte detection chip of the blood cell analysis chip of the embodiment of the present invention.The basophilic granulocyte detection chip of the embodiment of the present invention is provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies.Liquid storage tank is used for storage of blood sample and dilution, and waste liquid pool is for storing the blood sample through detecting, and aperture is for calculating the Effective Numerical of leucocyte bare nucleus and basophilic granulocyte.Wherein, liquid storage tank quantity is at least one, is respectively used to store anticoagulation, special basophilic granulocyte dilution and sheath fluid, and in embodiments of the present invention, basophilic granulocyte detection chip used diluent is available reagent.The detailed process that basophilic granulocyte detection chip carries out detecting is: anticoagulation sample introduction is to S2 liquid storage tank, special basophilic granulocyte dilution sample introduction is to R3 liquid storage tank, quantitative anticoagulation and quantitative dilution flow out from respective liquid storage tank and realize mixing, special basophilic granulocyte reagent is by the leucocyte removing cell membrane except basophilic granulocyte, make its bare nucleus and smaller volume, and erythrocytolysis, only basophilic granulocyte keeps original state, volume is obviously greater than the leucocyte of other types, sheath fluid sample introduction is to R8 liquid storage tank, treated blood sample is under the effect of quantitative sheath fluid stream, leucocyte is in line and one by one by detection zone A2, A2 upper and lower two ends in detection zone apply steady current, detect (namely each cell produces the electronic impulse proportional with cell volume) through electrical impedance and flow into waste liquid pool W2, according to threshold value setting through signal processing system Treatment Analysis, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical more accurately.In embodiments of the present invention, basophilic granulocyte detection chip adopts biological dissolution technology and electrical impedance method to detect basophilic granulocyte, further raising detection perform, can also adopt optical detection to detect, and dosing assemblies C8 to C11 is used for making corresponding fluids in each liquid storage tank quantitative.
Referring to Fig. 4, is the structural representation of the red blood cell of the blood cell analysis chip of the embodiment of the present invention, blood platelet or haemoglobin detection chip.Red blood cell, blood platelet or haemoglobin detection chip are provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies.Liquid storage tank is used for storage of blood sample and dilution, and waste liquid pool is for storing the blood sample through detecting, and detection zone is used for making red blood cell by carrying out laser irradiation during detection zone.Wherein, liquid storage tank quantity is at least one, is respectively used to store anti-freezing blood sample, dilution and sheath fluid.Red blood cell, blood platelet or haemoglobin detection chip detect red blood cell and adopt two multi-angle laser method, microcyte, bib and blood platelet are more easily differentiated, greatly improve accuracy and the precision of blood platelet mensuration, and adopt the direct Measuring hemoglobin of red blood cell scattered light.The detailed process that red blood cell and the red analysis chip of blood platelet carry out detecting is: anticoagulation sample introduction is to liquid storage tank S3, special dilution sample introduction is to liquid storage tank R4, quantitative anticoagulation and quantitative dilution flow out from respective liquid storage tank and realize mixing, sheath fluid sample introduction is to liquid storage tank R9, treated blood sample is under the effect of quantitative sheath fluid stream, red blood cell is in line and flows into waste liquid pool W3 by detection zone A3 one by one, laser irradiates each red blood cell scanning through detection zone A3, and low angle scattered light 2.8 ° is for measuring cell volume; High angle scattered light 7 ° measures each endoerythrocytic hemoglobin concentration (MCH) and content of hemoglobin mean value MCHC, and calculates the haemoglobin dispersion of distribution (HDW) value in red blood cell.Platelet count analysis have employed two-dimentional optical triangulation method too, high angle scattered light 7 ° can measure cell refractive index (RI), low angle scattered light 2.8 ° can measure cell size, two-dimensional scattering figure draws platelet counts and correlation parameter, blood platelet of all sizes in 1-60fl can be measured, also can measure blood platelet content content.Dosing assemblies C12 to C15 is used for making corresponding fluids in each liquid storage tank quantitative.
In the blood cell analysis chip embodiment of invention, sample introduction can adopt Micropump, electrokinetic injection, positive pressure to drive the various ways such as sample introduction, Ngatively pressurized sampling, electric osmose sample introduction, and chip can by quartz, glass, monocrystalline silicon, macromolecule polymeric material as making such as polymetylmethacrylate, polydimethylsiloxane, polycarbonates; In addition, in order to alleviate or avoid chip microchannel surface to Hemadsorption, chip microchannel inside surface can through ad hoc fashion modification, or chip material modification, or adds suitable adjuvant in solution.
Referring to Fig. 5, is the structural representation of the cellanalyzer of the embodiment of the present invention.The cellanalyzer of the embodiment of the present invention comprises blood cell analysis chip, detecting unit and signal processing system.Blood cell analysis chip, detecting unit are connected successively with signal processing system, blood cell analysis chip is for carrying haemocyte, detecting unit is used for applying laser beam to detection zone and irradiates haemocyte, signal processing system applies steady current to the upper and lower two ends of aperture, detect each cell and produce the electronic impulse proportional with cell volume, according to the threshold value of setting, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical more accurately; Or described signal processing system adopts high angle scattered light measure cell peroxidase content situation, endoerythrocytic hemoglobin concentration, content of hemoglobin mean value or measure cell refractive index, described signal processing system adopts low angle scattered light to measure cell volume or size.
Referring to Fig. 6, is the embody rule structural representation of the cellanalyzer of the embodiment of the present invention.The cellanalyzer of the embodiment of the present invention comprises collimation lens 2, condenser lens 3, spectroscope 4, diaphragm 5, photodetector 6 and blood cell analysis chip 7.Blood cell analysis chip 7 is blood cell analysis chip described in Fig. 1, for carrying haemocyte 8, light source 1 is irradiated to the haemocyte 8 of carrying on blood cell analysis chip 7 successively by collimation lens 2, condenser lens 3, then by collimation lens 2 and spectroscope 4, the other two ends of collimation lens 2 and spectroscope 4 are all connected with diaphragm 5 and photodetector 6.The cellanalyzer of the embodiment of the present invention is except being applied to medical industry, every relate to requirement measurement of species particle diameter and liquid in atomic quantity carry out quantitatively and qualitatively analyzing, can measure with this instrument, namely this instrument can develop into laser particle size analyzer, such as, in physico-chemical analysis in pure water, measure the content of its impurity and bacterium; The measurement of the degree of purity of various industrial high purity liquid, demarcate, to microelectronic component, the production of integrated circuit, quality control, pharmaceutical industry and chemical industry, Food Hygiene Surveillance etc. have realistic meaning.
Referring to Fig. 7, is the process flow diagram of the blood cell analysis method of the embodiment of the present invention.The blood cell analysis method of the embodiment of the present invention comprises:
Step 100: utilize laser light scattering and peroxidase staining technique to classify to cell;
In step 100, utilize laser light scattering and peroxidase staining technique to carry out classification to cell to be specially: anticoagulation, dilution, clean-out system and formaldehyde isotonic liquid difference sample introduction are to liquid storage tank S1, R1, R2, whole blood carries out suitably dilution and the short time hatches, now cell is destroyed by clean-out system, leucocyte slurry endoenzyme is fixed, by liquid storage tank R5, R6 mixes with hydrogen peroxide and four chloro-naphthols and heats respectively, now intracellular peroxidation thing enzyme decomposition of hydrogen peroxide produces oxonium ion, oxonium ion makes four chloro-naphthols develop the color and precipitate and is positioned enzyme reaction position, sheath fluid sample introduction is to liquid storage tank R7, through the blood sample of chemical staining under the effect of sheath fluid stream, leucocyte is in line and flows into waste liquid pool W1 by detection zone A1 through laser beam irradiation one by one, be classified due to light scattering and cell size difference.Appears as negative reaction without black-and-blue particle in cell after dyeing, occur that the black particle of fine particle or sparse distribution is weak positive reaction, occur that the thick and intensive particle of black is strong positive reaction.
All kinds of leucocyte to the reaction of peroxidase is: early stage myeloblast is for negative, each stage that early young grain is later all contains peroxidase, and strengthen gradually along with its content of maturation of cell, eosinophil has the strongest peroxidase, neutrophil leucocyte contains stronger peroxidase, and basophilic granulocyte is not containing this enzyme; In monocyte system, except the early stage primitive stage, inmature monokaryon and monocyte there will be more weak peroxidase reaction; Lymphocyte, erythroneocytosis, megacaryocyte etc. are all peroxidase negative reaction.To sum up, in blood five kinds of leukocytic peroxidase activities put in order into: eosinophil > neutrophil leucocyte > monocyte, lymphocyte and basophilic granulocyte are all without peroxidase.Due to after enzyme reaction in intensity difference (feminine gender, the weak positive, strong positive) and cell volume difference in size, when cell passes through detection zone A1 through laser beam irradiation, produce the scattered light of varying strength, each cell produces histochemical staining result and light scattering result two signals.
At the scatter diagram that peroxidase detection chip produces, transverse axis represents the cell peroxidase content situation obtained 7 ° of laser light scattering absorptances, and peroxidase strong positive cell is positioned at right-hand member; The longitudinal axis is the cell volume size that low angle scattered light 2.8 ° is measured, and the expression scattered light signal being positioned at top is strong, and cell volume is large.Except basophilic granulocyte, the cell of each type is recorded in specific position according to their feature, eosinophil, neutrophil leucocyte, monocyte and lymphocyte can be distinguished in setting range, and calculate total white blood cells and classification in conjunction with basophilic granulocyte detection chip result.To sum up, cell is divided into five classes by peroxidase detection chip: the 1) eosinophil of the most strong positive of peroxidase; 2) neutrophilic segmented granulocyte of peroxidase strong positive; 3) comparatively large, the p+ monocyte of peroxidase of volume; 4) lymphocyte of small volume, peroxidase feminine gender; 5) volume is greater than lymphocyte and the maxicell that is unstained of peroxidase feminine gender, and this type of cell increases prompting naivety or original various types of cells may occur.
Step 200: adopt biological dissolution technology and electrical impedance method to detect basophilic granulocyte;
In step 200, employing biological dissolution technology and electrical impedance method detect basophilic granulocyte detailed process: anticoagulation sample introduction is to liquid storage tank S2, special basophilic granulocyte dilution sample introduction is to liquid storage tank R3, quantitative anticoagulation and quantitative dilution flow out from respective liquid storage tank and realize mixing, special basophilic granulocyte reagent is by the leucocyte removing cell membrane except basophilic granulocyte, make its bare nucleus and smaller volume, and erythrocytolysis, only basophilic granulocyte keeps original state, volume is obviously greater than the leucocyte of other types, sheath fluid sample introduction is to liquid storage tank R8, treated blood sample is under the effect of quantitative sheath fluid stream, leucocyte is in line and one by one by detection zone A2, A2 upper and lower two ends in detection zone apply steady current, detect (namely each cell produces the electronic impulse proportional with cell volume) through electrical impedance and flow into waste liquid pool W2, according to threshold value setting through signal processing system Treatment Analysis, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical more accurately.
Step 300: adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin.
In step 300, low angle scattered light (1 °-3 °) is for measuring erythrocyte volume, high angle scattered light (5 °-15 °) measures each endoerythrocytic hemoglobin concentration (MCH) and content of hemoglobin mean value MCHC, and calculates the haemoglobin dispersion of distribution (HDW) value in red blood cell.In embodiments of the present invention, adopt low angle scattered light 2.8 ° for measuring cell volume; High angle scattered light 7 ° measures each endoerythrocytic hemoglobin concentration (MCH) and content of hemoglobin mean value MCHC, and calculates the haemoglobin dispersion of distribution (HDW) value in red blood cell.Platelet count analysis have employed two-dimentional optical triangulation method too, high angle scattered light (5 °-15 °) can measure cell refractive index (RI), low angle scattered light (1 °-3 °) can measure cell size, in embodiments of the present invention, adopt high angle scattered light 7 ° to measure cell refractive index (RI), low angle scattered light 2.8 ° can measure cell size.
The blood cell analysis chip of the embodiment of the present invention, analyser and analytical approach carry out somatotype to haemocyte in anticoagulation, have that structure is simple, volume is little, cost is low, easy to operate, easy care, easily transport, chip mistake and the advantage such as discardable, meet the demand for development of analytical instrument microminiaturization, integrated and portability, be applicable to the uses such as hospital, clinic, community and individual family.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a blood cell analysis chip, it is characterized in that, comprise: leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip, described leukocyte analysis chip comprises peroxidase detection chip and basophilic granulocyte detection chip, described peroxidase detection chip utilizes laser light scattering and peroxidase staining technique to carry out cell classification, described basophilic granulocyte detection chip adopts biological dissolution and electrical impedance method to detect basophilic granulocyte, described red blood cell, blood platelet or hemoglobin analysis chip adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin,
Peroxidase detection chip is provided with liquid storage tank, waste liquid pool W1, detection zone and multiple dosing assemblies, described liquid storage tank comprises liquid storage tank S1, liquid storage tank R1, liquid storage tank R2, liquid storage tank R5, liquid storage tank R6 and liquid storage tank R7, liquid storage tank S1 is for storing anticoagulation, liquid storage tank R1 is used for store washing liquid, liquid storage tank R2 is for storing formaldehyde isotonic liquid, liquid storage tank R5 is for storing hydrogen peroxide, liquid storage tank R6 is for storing four chloro-naphthols, liquid storage tank R7 is for storing sheath fluid, described peroxidase detection chip is also provided with first fluid passage, second fluid passage, 3rd fluid passage, 4th fluid passage, 5th fluid passage, 6th fluid passage and sense channel, liquid storage tank S1 is communicated with waste liquid pool W1 with sense channel by first fluid passage, liquid storage tank R1 is communicated with waste liquid pool W1 with sense channel by second fluid passage, liquid storage tank R2 is communicated with waste liquid pool W1 with sense channel by the 3rd fluid passage, second fluid passage and the 3rd fluid passage and sense channel cross at same position, liquid storage tank R5 is communicated with waste liquid pool W1 with sense channel by the 4th fluid passage, liquid storage tank R6 is communicated with waste liquid pool W1 with sense channel by the 5th fluid passage, 4th fluid passage and the 5th fluid passage and sense channel cross at same position, liquid storage tank R7 is communicated with waste liquid pool W1 with sense channel by two article of the 6th fluid passage, article two, the 6th fluid passage and sense channel cross at same position, second fluid passage and the 3rd fluid passage are in the position that crosses of sense channel, 4th fluid passage and the 5th fluid passage are in the position that crosses of sense channel, article two, the 6th fluid passage is successively set on sense channel in the position that crosses of sense channel, and second fluid passage and the 3rd fluid passage in the position that crosses of sense channel near liquid storage tank S1, article two, the 6th fluid passage in the position that crosses of sense channel near waste liquid pool W1, multiple dosing assemblies is located at described first fluid passage respectively, second fluid passage, 3rd fluid passage, 4th fluid passage, on 5th fluid passage and the 6th fluid passage, described waste liquid pool is for storing the blood sample through detecting, described detection zone carries out laser beam irradiation for making the leucocyte of blood sample through detection zone, classified by light scattering and the different blood sample to detecting of cell size, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
2. blood cell analysis chip according to claim 1, it is characterized in that, described basophilic granulocyte detection chip is provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies, described liquid storage tank is used for storage of blood sample and dilution, described waste liquid pool is for storing the blood sample through detecting, the cell that described detection zone is used for blood sample adopts electrical impedance technology to detect according to cell size through detection zone, the Effective Numerical of leucocyte bare nucleus and basophilic granulocyte is calculated by electronic impulse size and number, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
3. blood cell analysis chip according to claim 1, it is characterized in that, described red blood cell, blood platelet or haemoglobin detection chip are provided with liquid storage tank, waste liquid pool, detection zone and dosing assemblies, described liquid storage tank is used for storage of blood sample and dilution, described waste liquid pool is for storing the blood sample through detecting, described detection zone carries out laser beam irradiation for making the leucocyte of blood sample through detection zone, classified by light scattering and the different blood sample to detecting of cell size, described dosing assemblies is used for making corresponding fluids in each liquid storage tank quantitative.
4. the blood cell analysis chip according to any one of claims 1 to 3, is characterized in that, described leukocyte analysis chip and red blood cell, blood platelet or hemoglobin analysis chip adopt material to comprise quartz, glass, monocrystalline silicon or macromolecule polymeric material; Described liquid storage tank sample introduction adopts Micropump, electrokinetic injection, positive pressure to drive sample introduction, Ngatively pressurized sampling or electric osmose sample introduction various ways.
5. a cellanalyzer, it is characterized in that, comprise blood cell analysis chip as claimed in claim 1, detecting unit and signal processing system, described blood cell analysis chip, detecting unit is connected successively with signal processing system, described blood cell analysis chip is for carrying haemocyte, described detecting unit is used for applying laser beam to detection zone and irradiates haemocyte, described signal processing system applies steady current to upper and lower two ends, detection zone, detect each cell and produce the electronic impulse proportional with cell volume, according to the threshold value of setting, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical accurately, or described signal processing system adopts high angle scattered light to measure cell peroxidase content situation, endoerythrocytic hemoglobin concentration, content of hemoglobin mean value or measurement cell refractive index, described signal processing system adopts low angle scattered light to measure cell volume or size.
6. adopt blood cell analysis chip as claimed in claim 1 to carry out a blood cell analysis method for blood cell analysis, it is characterized in that, comprising:
Step a: utilize laser light scattering and peroxidase staining technique to classify to cell;
Step b: adopt biological dissolution technology and electrical impedance method to detect basophilic granulocyte;
Step c: adopt two multi-angle laser method to detect red blood cell and blood platelet, and adopt red blood cell optical scattering method to detect haemoglobin;
Described step a comprises: sample introduction is to liquid storage tank S1, R1, R2 respectively for anticoagulation, dilution, clean-out system and formaldehyde isotonic liquid, and whole blood carries out suitably dilution and the short time hatches; Mix and heat with hydrogen peroxide and four chloro-naphthols through liquid storage tank R5, R6 respectively, intracellular peroxidation thing enzyme decomposition of hydrogen peroxide produces oxonium ion, and oxonium ion makes four chloro-naphthols develop the color and precipitate and is positioned enzyme reaction position; Sheath fluid sample introduction is to liquid storage tank R7, and through the blood sample of chemical staining under the effect of sheath fluid stream, leucocyte is in line and successively by detection zone A1; Flow into waste liquid pool W1 through laser beam irradiation, classify according to light scattering and cell size difference.
7. blood cell analysis method according to claim 6, it is characterized in that, described step b comprises: anticoagulation sample introduction is to liquid storage tank S2, special basophilic granulocyte dilution sample introduction is to liquid storage tank R3, quantitative anticoagulation and quantitative dilution flow out from respective liquid storage tank and mix, sheath fluid sample introduction is to liquid storage tank R8, treated blood sample is under the effect of quantitative sheath fluid stream, leucocyte is in line and successively by count detection district A2, A2 upper and lower two ends in detection zone apply steady current, detect through electrical impedance and flow into waste liquid pool W2, according to threshold value setting through signal processing system Treatment Analysis, distinguish leucocyte bare nucleus and basophilic granulocyte, provide total white blood cells and basophilic granulocyte Effective Numerical accurately.
8. blood cell analysis method according to claim 6, it is characterized in that, in described step c, described pair of multi-angle laser method detects to comprise and uses low angle scattered light and high angle scattered light, and wherein, described low angle scattered light is for measuring erythrocyte volume, high angle scattered light measures each endoerythrocytic hemoglobin concentration and content of hemoglobin mean value, on platelet count is analyzed, high angle scattering optical power measurement cell refractive index, low angle scattering optical power measurement cell size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310372923.5A CN103472034B (en) | 2013-08-23 | 2013-08-23 | A kind of blood cell analysis chip, analyser and analytical approach |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310372923.5A CN103472034B (en) | 2013-08-23 | 2013-08-23 | A kind of blood cell analysis chip, analyser and analytical approach |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103472034A CN103472034A (en) | 2013-12-25 |
| CN103472034B true CN103472034B (en) | 2015-10-28 |
Family
ID=49796974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310372923.5A Active CN103472034B (en) | 2013-08-23 | 2013-08-23 | A kind of blood cell analysis chip, analyser and analytical approach |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103472034B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442323A (en) * | 2016-11-01 | 2017-02-22 | 陆丽君 | New clinical laboratory blood analysis instrument |
| CN106845622B (en) * | 2017-01-22 | 2019-04-23 | 江西特康科技有限公司 | Platelet count method and system |
| CN110226083B (en) * | 2017-02-17 | 2022-12-20 | 深圳迈瑞生物医疗电子股份有限公司 | Erythrocyte fragment recognition method and device, blood cell analyzer and analysis method |
| CN107219195B (en) * | 2017-05-23 | 2019-07-23 | 山东中医药大学附属医院 | A kind of blood leucocyte detection device and method |
| WO2019206312A1 (en) | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | Method for giving alarm about abnormality in sample analyzer, system, and storage medium |
| CN111801568A (en) * | 2018-04-28 | 2020-10-20 | 深圳迈瑞生物医疗电子股份有限公司 | Method and system for determining platelet concentration |
| WO2019206310A1 (en) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | Blood analysis method, blood analysis system, and storage medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185001A (en) * | 2005-03-31 | 2008-05-21 | C2诊断公司 | Method for analyzing a blood sample and apparatus and reagent for implementing said method |
| CN103175950A (en) * | 2011-12-20 | 2013-06-26 | 中国科学院深圳先进技术研究院 | Hemocyte analysis chip and system for using chip thereof |
| CN103170377A (en) * | 2011-12-20 | 2013-06-26 | 中国科学院深圳先进技术研究院 | Hemocyte analysis chip and system for using chip thereof |
-
2013
- 2013-08-23 CN CN201310372923.5A patent/CN103472034B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185001A (en) * | 2005-03-31 | 2008-05-21 | C2诊断公司 | Method for analyzing a blood sample and apparatus and reagent for implementing said method |
| CN103175950A (en) * | 2011-12-20 | 2013-06-26 | 中国科学院深圳先进技术研究院 | Hemocyte analysis chip and system for using chip thereof |
| CN103170377A (en) * | 2011-12-20 | 2013-06-26 | 中国科学院深圳先进技术研究院 | Hemocyte analysis chip and system for using chip thereof |
Non-Patent Citations (2)
| Title |
|---|
| 五分类法血细胞分析仪测定原理和散点图特征;张时民;《中国医疗器械信息》;20081230;第14卷(第12期);第4页右栏第10-39行，第5页，第6页右栏倒数第1段，图10 * |
| 血细胞分析仪的工作原理及其近期发展;姜穗;《医疗设备信息》;20040430(第4期);第38页右栏第2段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103472034A (en) | 2013-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103472034B (en) | A kind of blood cell analysis chip, analyser and analytical approach | |
| CN103471982B (en) | A kind of blood cell analysis chip, analyser and analytical approach | |
| Vembadi et al. | Cell cytometry: Review and perspective on biotechnological advances | |
| CN103471980B (en) | A kind of chip type blood cell analyzer and method | |
| JP3707620B2 (en) | Reticulocyte analysis method and apparatus using light scattering technology | |
| CN102300641B (en) | Microfluidic device for full blood count | |
| US4747685A (en) | Biological microparticle inspection apparatus | |
| US20190101486A1 (en) | Apparatus and Methods for Cellular Analysis | |
| US20120214224A1 (en) | Flow based clinical analysis | |
| US20060250604A1 (en) | Blood analyzer, sample analyzer, and flow cytometer | |
| EP2694960B1 (en) | Identifying and enumerating early granulated cells (egcs) | |
| CN103472216A (en) | Hemocyte analysis chip, analyzer and analysis method | |
| CN102016577A (en) | Blood analyzer, blood analysis method and hemolytic agent | |
| Weissenbacher et al. | Evaluation of a novel haematology analyser for use with feline blood | |
| JP2013235016A (en) | Transducer module | |
| JPWO2009136573A1 (en) | Blood analyzer, blood analysis method, hemolyzing agent and staining agent | |
| Longanbach et al. | Automated blood cell analysis | |
| CN108732338A (en) | A kind of classification of compatible leucocyte five and leucocyte three divide the cellanalyzer and test method of group | |
| CN107976397B (en) | Hemolytic agent and preparation method thereof | |
| JP4884049B2 (en) | Megakaryocyte counting method and apparatus | |
| US20220082551A1 (en) | Sample analyzing method and sample analyzer | |
| RU2803025C2 (en) | Method for analyzing biological sample containing biological cells and analyzing device for implementing method of analysis | |
| EP4187228A1 (en) | System, apparatus, and method for measuring erythrocyte sedimentation rate | |
| Fritsma | An overview of clinical laboratory hematology | |
| Kausar et al. | Frequency of Causes of Spurious Platelets Count on Routine Complete Blood Count by an Automated Hematology Cell Analyser |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: You Fan Inventor after: Li Fangfang Inventor after: Zhou Shumin Inventor after: Fan Jianping Inventor after: Huang Shi Inventor after: Xue Guangzhou Inventor before: Li Fangfang Inventor before: You Fan Inventor before: Zhou Shumin Inventor before: Huang Shi Inventor before: Xue Guangzhou |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LI FANGFANG YOU ZHOU SHUMIN HUANG SHI XUE GUANGZHOU TO: YOU LI FANGFANG ZHOU SHUMIN FAN JIANPING HUANG SHI XUE GUANGZHOU |
|
| C14 | Grant of patent or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: You Fan Inventor after: Li Fangfang Inventor after: Zhou Shumin Inventor after: Fan Jianping Inventor after: Huang Shi Inventor before: You Fan Inventor before: Li Fangfang Inventor before: Zhou Shumin Inventor before: Fan Jianping Inventor before: Huang Shi Inventor before: Li Fangfang Zhou Shumin Fan Jianping Xue Guangzhou Huangshi tour fan |
|
| COR | Change of bibliographic data | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: You Fan Inventor after: Zhou Shumin Inventor after: Li Fangfang Inventor after: Fan Jianping Inventor after: Huang Shi Inventor after: Xue Guangzhou Inventor before: You Fan Inventor before: Li Fangfang Inventor before: Zhou Shumin Inventor before: Fan Jianping Inventor before: Huang Shi |
|
| CB03 | Change of inventor or designer information | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170207 Address after: Lin Xi Lu Dragon Dragon Street Industrial Park in Longgang District of Shenzhen City, Guangdong province 518172 No. 1 Building 2 layer 210 Patentee after: Shenzhen Zhongke Technology Co., Ltd. Address before: Lin Xi Lu Dragon Dragon Street Industrial Park in Longgang District of Shenzhen City, Guangdong province 518172 No. 1 Building 1 layer 101,2 layer 201-209 Patentee before: Shenzhen Zhongke Qianghua Technology Co,. Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170426 Address after: 518172 Guangdong, Shenzhen, Longgang District, Longgang street, Feng Yi Road, No. 3, building 301-305, 34 Patentee after: Shenzhen Zhongke Intelligent Technology Co., Ltd. Address before: Lin Xi Lu Dragon Dragon Street Industrial Park in Longgang District of Shenzhen City, Guangdong province 518172 No. 1 Building 2 layer 210 Patentee before: Shenzhen Zhongke Technology Co., Ltd. |
|
| TR01 | Transfer of patent right |