CN106168554B - A kind of reagent for hemocyte analyzers - Google Patents
A kind of reagent for hemocyte analyzers Download PDFInfo
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- CN106168554B CN106168554B CN201610109709.4A CN201610109709A CN106168554B CN 106168554 B CN106168554 B CN 106168554B CN 201610109709 A CN201610109709 A CN 201610109709A CN 106168554 B CN106168554 B CN 106168554B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
Abstract
The invention discloses a kind of reagent for hemocyte analyzers, the reagent includes at least one alkali metal salt with reducing power, at least one buffer and at least one alkali metal salt.The sheath stream dilution that the reagent can be used as cellanalyzer uses, the content of dissolved oxygen in reagent can be effectively reduced, reduce the release of reagent dissolved gas caused by storage and transport process, guarantee the stability of instrument background, suitable pH, conductivity and osmotic pressure are provided simultaneously for the detection sample of cellanalyzer, guarantee that the form of sample cell is complete, haemocylolysis does not occur.Also disclose the production technology of the reagent.
Description
Technical field
The present invention relates to a kind of blood cell analysis reagent and its application methods, more particularly, it relates to can be with
The reagent used as cellanalyzer sheath stream diluent and the preparation method using the reagent.
Background technique
In the 1950s, Mr. Kurt in the U.S. has applied for the patent of invention of particle counting technology, it is basis
The non-conducting characteristic of haemocyte when the particle to suspend in the electrolytic solution is passed through small bore tube with electrolyte, replaces same volume
Electrolyte, cause inside and outside small bore tube two resistance between electrode that transient change occurs in the circuit of constant current design, generate current potential
Pulse.The size and number of pulse signal are directly proportional to the size and number of particle, which is commonly known as Kurt
Principle.Although the appearance of various new technologies is with greatly having pushed cellanalyzer fast-developing, each quasi-instrument is still used
Coulter principle carries out the counting and stereometry of haemocyte as the basis of blood count and cubing.Develop to the present
It, blood cell analysis has become detection project conventional in clinical detection, it can in blood red blood cell, leucocyte,
The parameters such as blood platelet and hemoglobin are detected and analyzed, and substantially increase the working efficiency of laboratory physician.
Can background count have important work as instrument basic performance indices, for get accurate detection result
With.Instrument is before each booting simultaneously, requires the inspection for carrying out background count, only its numerical value in allowed limits,
Believable testing result can be exported during subsequent detection.There are numerous liquid lines and valves in instrument, when these portions
Part is contaminated, will appear interference signal by Electromagnetic Interference or without good ground connection, causes background count unqualified.Make
Contain dissolved oxygen in reagent, it can be released with the extension of storage and transportation time or the variation of ambient temperature, adherency
In instrument pipeline, into instrument sense channel after can be arrived as pattern detection, there is deviation so as to cause count results.
Domestic patent CN1205436 discloses a kind of blood analysis instrument dilution and preparation method thereof.The original of the dilution
Material contains NaCl, KCl, H3BO3、Na2B4O7.10H2O, EDTA-2Na, chlorhexidine acetate etc. have certain bacteriostasis, raw
Production. art is simple.
Domestic patent CN1834612 discloses a kind of multi-functional dilution and preparation method thereof.The dilution includes second two
Amine tetraacetate, alkali metal sulfates, alkali metal chlorizated salt, phosphate buffer, the alkyl sulfate of alkali metal, polyoxyethylene
The components such as type nonionic surfactant.The dilution can make the isometric spheroidization of red blood cell, not vulnerable to red when instrument detects
The influence of cell volume variation, testing result are more acurrate reliable.
Domestic patent CN101451931B discloses a kind of blood dilution liquid.The dilution include purine compound,
The components such as alkali metal salt and buffer.The dilution can provide suitable conductance and osmotic pressure environment, guarantee in blood cell
Dilution is provided when analyzer detects and stablizes the effect of cell, and can make untreated new blood at room temperature
Sample is stablized placement 2 hours or more.
Domestic patent CN103323318A discloses a kind of blood cell analyzer dilution.The dilution includes alkali metal chlorine
The components such as compound, ethylenediamine tetra-acetic acid, pyridiniujm, pH buffer tune.It is not accurate enough that it solves the problems, such as that leucocyte three is classified.
Domestic patent CN103743616A discloses a kind of blood analyser dilution.The dilution includes: sodium chloride, sulphur
The components such as sour sodium, edetate, potassium chloride, imidazoles, purine compound.The dilution be able to maintain whole blood sample and
The stability of leukocyte differential count and MCV and blood sample substance in pre-dilution sample, while blood sample after variation of ambient temperature
MCV value be still in stable level.
Domestic patent CN101021456 discloses a kind of production method of tripartite group blood cell analyser dilution, raw
The step of production. art includes pure water preparation, the preparation of buffer, dissolution is prepared, constant volume is prepared, it is filling to filter.Select sodium chloride
It is the main component of reagent with sodium sulphate, reduces the cost of reagent.Without addition preservative in the patent claims reagent, but
It then joined formaldehyde in purged and packed, threat not only constituted to the health of operator in this way, but also formaldehyde is as preservative
It is known.
Domestic patent CN1215168 discloses a kind of reagent composition without cyanide ion and a kind of for measuring blood
The method of hemoglobin concentration in liquid sample.The reagent is living by the surface of or mixtures thereof at least one quaternary ammonium salt, pyridine
Property agent and a kind of antioxidant that the hemoglobin of release can be converted into hemochromogen constitute, antioxidant therein is anti-bad
Or mixtures thereof hematic acid, phosphorous acid, sodium sulfite, sodium hydrogensulfite etc..
United States Patent (USP) US4322313 discloses a kind of multipurpose dilution.The dilution is by alkali metal salt, phosphoric acid buffer
The composition such as liquid, edetate, lithium chloride and Phenoxyethanol., using Phenoxyethanol as antibacterial agent, lithium chloride then rises for it
To the effect for stablizing erythrocyte volume.
United States Patent (USP) US4968629 discloses a kind of dilution in semi-automatic blood analyser.The dilution by
The composition such as sodium sulphate, phosphoric acid buffer system, pH is in 6.0~8.0 ranges.The reagent is not in use to the survey of leucocyte
Amount has an impact.
United States Patent (USP) US5227304 discloses a kind of reagent system formed including isotonic dilution and detergent.Its
In dilution be made of imidazole buffer, antimicrobial, inorganic salts etc..Wherein EDTA is as chelating agent, and enhances this
The anti-microbe ability of dilution can be used as blood count and the reagent application of magnitude classification.
Reagent disclosed above can provide the environment such as suitable pH and osmotic pressure for the detection of blood sample, count sample
Red blood cell, number of platelets.The composition of reagent includes buffer system, alkali metal salt etc..But without reference to how eliminating or
Person reduces the influence that dissolved oxygen counts instrument background in reagent, and the method and reagent for improving testing result accuracy exist
It stores and gas is caused to enter in reagent through packaging material as time went in, formed after varying with temperature release
The influence that bubble detects instrument.Therefore the problem of needing for current reagent, is analyzed and is studied, and is proposed effective
Solution.
Summary of the invention
In its natural state, a certain amount of oxygen is always contained in water, this is because the oxygen in air has one in water
Fixed dissolubility.Usually under 1 atmospheric pressure, in 0 DEG C of water, dissolved oxygen 6.95mg/L；In 20 DEG C of content of oxygen dissolved in water
For 4.34mg/L, as the temperature rises, its solubility in water is gradually decreased, such as when the temperature of water is increased to 80 DEG C,
The content of dissolved oxygen is then reduced to 1.4mg/L.This changing rule defers to Henry's law (Henry'slaw), the statement of the law
Are as follows: " under isothermal and isobaric, the solute is flat in certain volatile solutes (normally gaseous) solubility in the solution and liquid level
The pressure that weighs is directly proportional." its formula is
Pg=Hx
In formula: H is Henry constant, and x is gas molar score solubility, and Pg is the partial pressure of gas.H can be good at table
Show the meltage of gas, it is suitable for the system of solubility very little.In this sense, Henry constant is the function of temperature,
It is unrelated with pressure.
Sheath stream diluent plays an important role in the normal and accurate operation of cellanalyzer.On the one hand, sheath stream
Dilution provides suitable pH, conductivity and osmotic pressure for detection sample, guarantees that the form of cell is complete, and haemolysis work does not occur
With；On the one hand it needs to detect the remaining substance of sample to last time and clean, guarantee the cleaning of sampling needle, pipeline and flow chamber,
Prevent cross contamination；Diluent will fill among pipeline during another further aspect instrument temporarily ceases operation, prevent extraneous dust
Equal foreign matters enter instrument, cause failure.
Water used in sheath stream diluent is purified water, after it have passed through multi task process, effectively removes the impurity in water, has
Machine object, heavy metal, the hardness for reducing water.In an aqueous medium, the pH of alkali metal salt, chelating agent, buffer and regulation system is added
In neutrality, it is available for the diluent of cellanalyzer.But there is great in use for this reagent system
Insufficient: 1) process for producing sheath stream dilution is to be put into material and pure water in reaction kettle by a certain percentage, passes through stirring
Mode all dissolves the material of addition, and oxygen can contact for a long time under the disturbance of blender with dilution, increases reagent
Middle dissolved oxygen content；PH, osmotic pressure and conductivity are also more paid close attention in the Testing index of sheath stream dilution, without examining simultaneously
Survey dissolved oxygen content.2) throughout the country due to the place to use dispersion of instrument, reagent needs to transport from manufacturer, and each
The temperature on ground has larger difference, and dissolved oxygen in transportational process is caused to be slowly increased in diluent, while the packaging material of reagent
The transmission for effectively preventing oxygen is also failed to, causes reagent dissolved oxygen in long-time storage and transportation to increase, is eventually exhibited as diluent and exists
Background count on instrument increases, and cannot provide accurate detection result.3) it in instrument use process, fills in internal duct
In diluent be not drained during instrument down time, but lasting filling is in pipeline.With the extension environment of time
In oxygen can enter in dilution through pipeline, then as temperature variation dissolved oxygen can gradually release to be formed it is small
Bubble is gathered in instrument pipeline inner wall, and the background count that this micro-bubble will lead to instrument is not up to standard, also will affect detection knot
Fruit count accuracy.
For the present invention precisely in order to meeting the needs of clinical examination, dissolved oxygen content in reduction sheath stream dilution prevents reagent
When storage and transportation and ambient temperature change, oxygen enters in reagent through packaging material and is slowly precipitated again, forms micro-bubble, enters
Instrument internal influences the accuracy of background count and pattern detection count results and the reagent developed.The reagent is in blood cell point
Suitable conductance and osmotic pressure environment are provided for detection sample in analyzer, guarantees to provide dilution when blood cell analyzer detects
The effect of cell is stablized in agent, while will test sample as carrier and importing in instrument, counting red corpuscles, leucocyte and blood platelet
Numerical value.
The present inventor repeatedly it was found that, by be added have reducing power alkali metal salt, can be effectively
The dissolved oxygen content in diluent is reduced, eliminates the increase of increased dissolved oxygen amount due to long-time storage and transportation of sheath stream dilution, together
When thoroughly eliminate through instrument pipeline enter penetrate oxygen, to guarantee the good operating status of instrument.The principle of the reaction is
Redox reaction occurs for the dissolved oxygen in alkali metal salt and water with reducing power, consumes the dissolved oxygen in water, simultaneously should
The reducing power of salt, which disappears, generates water-soluble alkali metal salts.
The alkali metal salt with reducing power include including sodium hydrogensulfite, potassium bisulfite, Hydros,
Sodium dithionite, potassium hyposulfite, sodium pyrosulfite, potassium metabisulfite, sodium sulfite, potassium sulfite compound and its group
It closes.Preferably scheme is using sodium hydrogensulfite, potassium bisulfite, sodium sulfite, potassium sulfite；More optimal solution is using Asia
Sodium sulphate, potassium sulfite.
It is learnt according to above-mentioned reaction principle by theoretical calculation, removes the dissolved oxygen of 1mg/L in sheath stream dilution, need
The alkali sulfite metal salt of 8mg/L.And common alkali sulfite metal salt contains the crystallization water of 7 molecules, then removing 1mg/L
Dissolved oxygen theoretically need the alkali sulfite metal salt of 16mg/L.The additional amount of alkali sulfite metal salt is more, mistake in reagent
Surplus amount is more, and the percentage of deoxygenation is higher, after additional amount reaches certain value.With the increasing of alkali sulfite metal salt additional amount
Add, deoxygenation percentage then no longer rises.Experiment discovery is slightly excessive when the amount of alkali sulfite metal salt, can play good go
Except dissolved oxygen effect.
Alkali sulfite metal salt is added merely at normal temperature, deoxygenation percentage maximum can achieve 94%.Temperature is to adding
It is critically important influence factor that alkali sulfite metal salt, which removes dissolved oxygen effect,.In identical alkali sulfite metal salt additional amount
Under, water temperature is higher, and the effect for removing dissolved oxygen is better；Under identical removal dissolved oxygen effect, water temperature is higher, then the Asia needed
The amount of alkali metal persulfates is fewer.When water temperature is increased to 40 DEG C, the content of dissolved oxygen can be reduced to 0mg/L substantially.
Influence of the pH to dissolved oxygen in removal sheath stream dilution is that equal amount alkali sulfite metal is added as pH value increases
The oxygen scavenging ability of salt can reduce.But in acid and alkaline pH range, respectively there is the maximum value of a deoxygenation percentage.
Quickly, sodium sulfite alkali metal salt is added about in the reaction speed of dissolved oxygen in sodium sulfite alkali metal salt and water
The equalization point that can reach reaction after 3min, extends the reaction time not at this time again and can increase deoxygenation percentage.
Another influence factor is reagent ion intensity.When salt content increase in temperature, one timing of pressure, water, can make
The solubility of gas in water reduces.This is because the electrostriction of ion pair water (refers to Ions Absorption pole with the increase of salinity
Property hydrone, be the phenomenon that hydrone forms the hydration layer closely arranged around it) reinforce, make the gap of water dissolvable gas
It reduces.
First group of sheath stream dilution of the present invention is divided into the alkali metal salt with reducing power, including sodium hydrogensulfite, Asia
Potassium acid sulfate, Hydros, sodium dithionite, potassium hyposulfite, sodium pyrosulfite, potassium metabisulfite, sodium sulfite,
Potassium sulfite compound and combinations thereof；Second group of the reagent is divided into inorganic alkaline metal salt, including sodium sulphate, potassium sulfate, chlorine
Change sodium, potassium chloride and combinations thereof；The third group of the reagent is divided into buffer, including phosphate buffer, boric acid-borax buffering
Liquid, citric acid-sodium citrate buffer solution, phthalic acid-hydrochloride buffer etc..
By the way that suitable alkali metal salt with reducing power is added in the present invention, on the one hand consume molten in reagent
Oxygen is solved, while the reducing power of the alkali metal salt disappears, and water-soluble metal salt is generated, so as to reduce sodium chloride, sulfuric acid
Sodium etc. is used to adjust the metal salt additional amount of the parameters such as reagent conductivity and osmotic pressure.
Another aspect of the present invention provides the production method of the reagent.In order to thoroughly by material dissolution, when generally passing through long
Between stirring achieve the goal, but this process will lead to the increase of oxygen meltage in reagent in environment.The present inventor passes through
Then the alkali gold of requirement is added using first preparing buffer solution and adjusting its pH into a certain range in many experiments discovery
Belong to the ionic strength that salt adjusts reagent, stirring finally, which is added, in the reproducibility alkali metal salt of calculation amount dissolves it thoroughly, detects
Filling production method is filtered after qualification, can achieve the content that dissolved oxygen in reagent is both effectively reduced, and guarantees the quality of product；
The consumption of the energy in reagent production process is reduced again.
Beneficial effects of the present invention: by the way that the alkali metal salt with reducing power, Ke Yiyou are added in sheath stream dilution
Effect ground reduces dissolved oxygen content in reagent, eliminates sheath stream dilution in the dissolved oxygen that production link and storage and transportation link are formed and shape is precipitated
Enter instrument internal pipeline at micro-bubble, influences instrument count accuracy；It is saturating to eliminate instrument ambient oxygen in stopping process
The dissolved oxygen that instrument pipeline enters in reagent is crossed, is slowly precipitated in instrument operation and influences instrument count results.Meanwhile it using
It first prepares buffer solution and is redissolved alkali metal salt, be eventually adding the production method of reproducibility alkali metal salt stirring and dissolving, Ke Yida
To the content that dissolved oxygen in reagent is both effectively reduced, guarantee the quality of product；The consumption of the energy in reagent production process is reduced again
Purpose.
These features of the invention and advantage and other feature and advantage, reference a specific embodiment of the invention it
After will become obvious.
Detailed description of the invention:
Fig. 1 is that the elimination dissolved oxygen ability of different reproducibility alkali metal salts compares figure.
Specific embodiment
Definition
Unless otherwise stated, term used herein has following meanings.
Term " alkali metal " used herein refers to the metallic element that family is belonged in the periodic table of elements, including sodium and
Potassium, they have many similar chemical property.
Term " reducing power " used herein refers to that in chemical reaction atom, molecule or ion lose the energy of electronics
Power.Element reduction belonging to the stronger particle of betatopic ability is stronger；Otherwise it is weaker, and its irreducibility is stronger.Also
Originality is for oxidisability.Substance with reducing power is oxidized easily.
Term " background count " used herein refer to use dilution as sample on cellanalyzer continuously into
Row is tested three times, takes the maximum value in test result three times as the value of background count.This numerical value is lower, shows the background of instrument
Counting is lower, and preferably zero, i.e., no reading value.
Blood cell analysis reagent of the invention
Reagent disclosed in this invention provides suitable conductance and osmotic pressure in blood cell analyzer for detection sample
Environment guarantees to provide the effect that diluent stablizes cell when blood cell analyzer detects, while will test sample as carrier
In this importing instrument, the numerical value of counting red corpuscles, leucocyte and blood platelet.
Inorganic alkaline metal salt
Inorganic alkaline metal salt in the present invention includes the combination of alkali metal sulfates and alkali metal chlorizated salt.These alkali metal
Salt can be improved the electric conductivity of solution, keep the isosmoticity of reagent, and maintain the balance of electrochemistry.Their presence can be molten
The abnormal protein in sample is solved, prevents them from being deposited on pipeline, plays the role of Dishwasher pipeline.Preferred alkali metal
Salt includes sodium sulphate, potassium sulfate, sodium chloride and potassium chloride, more preferably sodium sulphate and sodium chloride.
Buffer
Buffer used in the present invention provides suitable pH value, including phosphate buffer, boron for blood testing sample
Acid-borate buffer solution, citric acid-sodium citrate buffer solution, phthalic acid-hydrochloride buffer etc..Preferred pH range is 4.5
To 8.5, more preferably 5.5 to 8.0, most preferably 6.5 to 7.5, namely in neutral range.
Embodiment
Embodiment provided below is only used for making the present invention further for example, and having no intention to specification
Make any restriction.
Unless otherwise stated, otherwise blood cell detection device used in following embodiment is Jia Sidaike Medical treatment device
The stream type cell analyzer of tool Co., Ltd production.
Consumption of the 1 reproducibility alkali metal salt of embodiment to pure oxygen in water
A clean beaker is taken, takes 1L pure water to pour into wherein with graduated cylinder correct amount, is placed on magnetic stirring apparatus, is adjusted
Revolving speed is 200r/min, and installs the dissolved oxygen meter after calibration.It is separately added into the reproducibility alkali gold of 1.0g precise
Belong to salt, detects the situation of change of dissolved oxygen in liquid after reacting different time.As a result (wherein dissolved oxygen contains as shown in Fig. 1
Amount is mg/L).
Each reproducibility alkali metal salt is observed to the consumption data of dissolved oxygen it can be found that each reproducibility alkali metal salt all may be used
With within the regular hour that the dissolved oxygen depletion in water is complete, and maintain the level.Do not occur with mixing time
Extend, ambient oxygen is re-dissolved in the case where causing oxygen content to rise in reagent.
The dissolved oxygen variation of the different storage and transportation time reagents of embodiment 2
Sheath stream dilution is prepared by following material ratio:
Compare embodiment 1
It will be filled in plastics Soft Roll after the filtering of prepared reagent accurate, put it into post package in outer packing, and do
Good corresponding label.Two same sections of production sharing are stored in reagent storage.First part is statically placed in warehouse, second part
10 hours were then shaken back and forth with the revolving speed of 100r/min on simulation traffic simulator at interval of 3 months, for simulating reagent
The traffic condition of product.Two batches reagent is broken a seal respectively after 6 months, 9 months, 12 months, 15 months and is taken out, detection examination
The variation of oxygen content in agent, test three times, take the average value of result.Data are as shown in table 1 below.
It can be seen that the standing of the reagent containing reproducibility alkali metal salt from upper table data to be placed in warehouse, by 15
After the time of the moon, the level that dissolved oxygen content therein maintains 0.48mg/L does not increase.It is primary through concussion in super-interval 3 months
Reagent, dissolved oxygen content therein slightly has increase.And the reagent without containing reproducibility alkali metal salt is belonged to due to oxygen content
Saturation state, so either standing placement different time, still oxygen content does not become significantly by way of the concussion of interval
Change, maintains the level of 6mg/L.
Influence of the different reagents of embodiment 3 to instrument background inspection
It is limited that the sheath stream dilution prepared in embodiment 2 and comparative example is respectively directed to Jia Sidaike medical instrument
In the flow cytometry analysis instrument of company's production, background count is carried out；Be then shut off after instrument 24H in optical viewer pipeline whether
There is micro-bubble precipitation, and carries out background count.As a result as shown in table 2 below.
By it has been observed that carrying out background test on instrument using reagent prepared by embodiment 2, result is 0, simultaneously
Instrument occurs in closing the pipeline after 24H without discovery micro-bubble, it is ensured that detects the accuracy of count results.And it adopts
It is 0.1 × 10 with leucocyte (WBC) numerical value in the background testing result of contrast agent9/ L, blood platelet (PLT) numerical value are 6 × 109/
L；Testing result after standby 24H is WBC0.1 × 109/ L, PLT are 8 × 109/ L, and can see on the pipeline of instrument sparse
Micro-bubble exist, by can just drive bubble after repeatedly rinsing, which not only adds the consumption of reagent, improve
Testing cost, and influence is produced on count results accuracy.
The different production methods of embodiment 4 reduce dissolved oxygen effect in reagent and compare
Using following production procedure reagent preparation:
(1) purified water of specified amount is added in a kettle, starts mixing plant, load weighted buffer material is added simultaneously
Being stirred to react and adjusting the pH of reagent to whole dissolutions is 6.0~6.5；
(2) be added requirement sodium chloride and sodium sulphate stir into reagent and all dissolve, for adjust reagent from
Sub- intensity；
(3) stirring is added in the reproducibility alkali metal salt of calculation amount dissolves it thoroughly, for consuming the dissolution in reagent
Oxygen；
(4) dissolved oxygen content and physical and chemical parameter for adjusting the detection reagent of pH to 7.0 of reagent, are then filtered filling.
It uses the production technology to prepare pH first as the buffer solution of slant acidity, the sodium chloride and sulphur of requirement is then added
Sour sodium increases the ionic strength of reagent, on the one hand can consume dissolved oxygen for reproducibility alkali metal salt and provide suitable reaction environment,
On the one hand solubility of the ambient oxygen in reagent is reduced, using effect is improved.It is eventually adding reproducibility alkali metal salt, Ke Yida
To the content that dissolved oxygen in reagent is both effectively reduced, guarantee the quality of product；The consumption of the energy in reagent production process is reduced again
Purpose.
Comparative example 2,3
It is made an explanation by above-mentioned each embodiment and specific embodiment to the present invention, but those skilled in the art can manage
Solution, without departing from present inventive concept and range, can to the present invention various modification can be adapted, change and replacement.
Claims (8)
1. a kind of cytometry reagent, the reagent be by:
(1) first group is divided at least one alkali metal salt with reducing power
(2) second groups are divided at least one inorganic alkaline metal salt
(3) the third group is divided at least one buffer composition；
The content of dissolved oxygen is lower than 1.0mg/L in the reagent.
2. cytometry reagent according to claim 1, wherein the alkali metal with reducing power
Salt include sodium hydrogensulfite, potassium bisulfite, sodium dithionite, potassium hyposulfite, sodium pyrosulfite, potassium metabisulfite,
Sodium sulfite, potassium sulfite compound and combinations thereof.
3. cytometry reagent according to claim 2, wherein the alkali metal salt with reducing power
For sodium hydrogensulfite, potassium bisulfite, sodium sulfite, potassium sulfite and combinations thereof.
4. cytometry reagent described in any one of -3 according to claim 1, wherein described have also proper energy
The usage amount of the alkali metal salt of power is 0.05-15g/L.
5. cytometry reagent according to claim 4, wherein the alkali metal salt with reducing power
Usage amount be 0.05-10g/L.
6. cytometry reagent described in any one of -3 according to claim 1, wherein the inorganic base metal
Salt includes sodium sulphate, potassium sulfate, sodium chloride, potassium chloride and combinations thereof.
7. cytometry reagent described in any one of -3 according to claim 1, wherein the buffer includes
Phosphate buffer, boric acid-borate buffer solution, citric acid-sodium citrate buffer solution, phthalic acid-hydrochloride buffer.
8. a kind of production technology of cytometry reagent, the technique include the following steps:
(1) purified water of specified amount is added in a kettle, starts mixing plant, load weighted buffer material is added and stirs
To whole dissolutions, the pH for adjusting reagent is 6.0~6.5 for reaction；
(2) inorganic alkaline metal salt that requirement is added stirs into reagent and dissolves it all, for adjusting the ion of reagent
Intensity；
(3) stirring is added in the reproducibility alkali metal salt of calculation amount dissolves it thoroughly, for reacting the dissolution in consumption reagent
Oxygen；
(4) pH for adjusting reagent is 6.5~7.5 ranges, is then filtered filling.
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| CN108414427A (en) * | 2017-02-10 | 2018-08-17 | 深圳市帝迈生物技术有限公司 | A kind of leucocyte classification reagent |
| CN107014664A (en) * | 2017-03-22 | 2017-08-04 | 安徽民泰医药科技有限公司 | Blood cell analysis dilution |
| CN107941679A (en) * | 2017-11-22 | 2018-04-20 | 中山市创艺生化工程有限公司 | A kind of reagent for hemocyte analyzers |
| CN107976354A (en) * | 2017-11-22 | 2018-05-01 | 中山市创艺生化工程有限公司 | A kind of reagent for hemocyte analyzers |
| CN108362868A (en) * | 2017-11-29 | 2018-08-03 | 南宁优康电子科技有限公司 | A kind of storage of reagent for hemocyte analyzers and preparation method |
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