CN107897558A - A kind of microorganism products for improving laying rate and increasing egg size and preparation method and application - Google Patents
A kind of microorganism products for improving laying rate and increasing egg size and preparation method and application Download PDFInfo
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- CN107897558A CN107897558A CN201711180210.3A CN201711180210A CN107897558A CN 107897558 A CN107897558 A CN 107897558A CN 201711180210 A CN201711180210 A CN 201711180210A CN 107897558 A CN107897558 A CN 107897558A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
The present invention relates to a kind of microorganism products for improving laying rate and increasing egg size and preparation method and application, belong to field of fodder.The microorganism products include propolis lotion, aloe, earthworm, spirulina and double benefit elements.Double benefit elements include bifidobacterium adolescentis and streptomyces coelicolor.The microorganism products are green safe, palatability is good, nutritive value is high, and dosage is few, and cost is low, effect stability.Its preparation method is：Aloe, earthworm and spirulina are crushed, obtains aloe powder, earthworm powder and spirulina powder, by proportioning mixing aloe powder, earthworm powder, spirulina powder and double benefit elements, obtains the first mixture, the first mixture of mixing and propolis lotion.This preparation method is simple, and condition controllability is good.Above-mentioned microorganism products are used for added in the feed of egg-laying bird, egg-laying bird growth can be promoted, strengthen its immunity, raising egg size and laying rate, improve breeding environment.
Description
Technical field
The present invention relates to field of fodder, and more particularly to a kind of microorganism products for improving laying rate and increasing egg size
And preparation method and application.
Background technology
As the raising of people's economic condition and the continuous of diet structure improve, to animal products such as meat, egg, breasts (milk)
Demand it is higher and higher, this requires aviculture develops to scale, industrialization direction, higher is proposed to laying hen production capacity
Requirement.
Laying rate height is the major criterion for weighing laying hen production level.Therefore, laying rate of laying hen is improved to meeting market
It is required that having and its important meaning.And the factor for influencing laying rate is very much, such as kind, season, feeding management, daily ration structure
Deng.
Due to the rise of large-scale cultivation, feeding management is not caught up with, and induces a large amount of diseases.For this reason, a large amount of antibiotic are indiscriminate
With, but the use of antibiotic can make livestock and poultry produce drug resistance, limit the growth and development of livestock and poultry and the quality of product.
Therefore have to look for green safe probiotics and carry out substitute antibiotics, disease preventing and treating, improves laying rate of laying hen
With the increase production performance such as egg size.
The content of the invention
It is an object of the invention to provide a kind of microorganism products for improving laying rate and increasing egg size, the Tiny ecosystem
Compound formulation is green safe, palatability is good, nutritive value is high, and dosage is few, and cost is low, effect stability.
The second object of the present invention is to provide a kind of preparation method of above-mentioned probiotics, and this method is simple, condition
Controllability is good.
The third object of the present invention is the application for providing above-mentioned probiotics, adds it to the feed of egg-laying bird
In, egg-laying bird growth can be promoted, strengthen its immunity, raising egg size and laying rate, improve breeding environment.
The present invention is solved its technical problem and is realized using following technical scheme：
The present invention proposes a kind of microorganism products for improving laying rate and increasing egg size, and above-mentioned probiotics includes
The propolis lotion of 15-25 parts by weight, the aloe of 15-25 parts by weight, the earthworm of 16-25 parts by weight, the spirulina of 12-24 parts by weight
With double benefit elements of 8-18 parts by weight.
Double benefit elements include bifidobacterium adolescentis and streptomyces coelicolor.
The present invention also proposes the preparation method of above-mentioned probiotics, comprises the following steps：Crush aloe, earthworm and spiral shell
Algae is revolved, obtains aloe powder, earthworm powder and spirulina powder, by proportioning mixing aloe powder, earthworm powder, spirulina powder and double benefit elements, is obtained
First mixture, the first mixture of mixing and propolis lotion.
Preferably, aloe powder, earthworm powder, the powder particle diameter of spirulina powder are 0.2-0.5cm.
Preferably, the first mixture with propolis lotion is mixed under conditions of 40-50 DEG C.
In addition, the invention also provides the application of above-mentioned probiotics, such as microorganism products can be used to add
Add in the feed of egg-laying bird.
Preferably, egg-laying bird includes any one in chicken, duck and goose.
The raising laying rate and microorganism products and its preparation side of increase egg size that present pre-ferred embodiments provide
Method and the beneficial effect of application are：
Propolis can adjust the activity of a variety of enzymes, and the rheum emodin in aloe can increase the activity of lipase, energy after the two collaboration
Play the role of improving appetite, stomach invigorating, improve digestive function, improve laying rate；Earthworm powder and spirulina powder are good albumen
Resource, can strengthen the immunity of egg-laying bird jointly with propolis, aloe, improve egg size.By adding double benefit elements, further coordinate
Mentioned component plays the effect for adjusting intestinal health and strengthen immunity.
Aloe powder, earthworm powder, spirulina powder and the plain four class particulate materials of double benefits are first mixed, it is then newborn with propolis again
Liquid mixes, and particulate species material can be made to be uniformly dispersed in propolis lotion, makes the microorganism products nutrition equilibrium finally obtained.
The microorganism products of gained are used for added in the feed of egg-laying bird, egg-laying bird can be promoted to give birth to
Length, its immunity that strengthens, improve egg size and laying rate, improve breeding environment.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase
Product.
Microorganism products of raising laying rate to the embodiment of the present invention and increase egg size and preparation method thereof below
It is specifically described with application.
Probiotics provided in an embodiment of the present invention, uses it for added in the feed of egg-laying bird, can promote
Egg-laying bird growth, strengthen its immunity, raising egg size and laying rate, improve breeding environment.
Optionally, above-mentioned egg-laying bird for example can be chicken, any one in duck and goose, the especially cultivation in chicken
Middle effect becomes apparent.
Specifically, in adding procedure, microorganism products for example can make an addition to egg fowl in the ratio of 0.5-2wt%
In the feed of class, such as can be 0.5wt%, 1wt% and 2wt%.It is preferred that added by 1wt% in the feed of egg-laying bird.Should
It can make probiotics contained in feed that there are excellent results under adding proportion, cost performance is optimal.
Probiotics in the present embodiment contains the propolis lotion of 15-25 parts by weight, the aloe of 15-25 parts by weight, 16-
Double benefit elements of the earthworm of 25 parts by weight, the spirulina of 12-24 parts by weight and 8-18 parts by weight.
Further, above-mentioned probiotics can for example include propolis lotion, the 18-22 parts by weight of 18-22 parts by weight
Aloe, the earthworm of 18-23 parts by weight, double benefit elements of the spirulina of 16-20 parts by weight and 12-14 parts by weight.
Further, above-mentioned probiotics can for example include propolis lotion, the reed of 20 parts by weight of 20 parts by weight
Luxuriant growth, the earthworm of 20.5 parts by weight, double benefit elements of the spirulina of 18 parts by weight and 13 parts by weight.
Wherein, steroidal compounds and terpenoid are contained in propolis lotion, can not only be adjusted a variety of in egg-laying bird body
The activity of enzyme, promotes energetic supersession, protein and nucleic acid synthesis and biomembrane transhipment of liver cell etc., but also with promotion
The effect of growth of animals or poultry and increase egg size.
Contained aloin and Anthraquinone have the function that defaecation, anti-inflammatory and stomach invigorating in aloe.Aloe-emodin
It can promote the secretion of intestinal fluid, increase the activity of lipase, improve digestive function, improve appetite and laying rate, reduce the death rate.
Earthworm and spirulina can effectively strengthen egg-laying bird rich in protein, amino acid, vitamin and mineral etc.
Immunity, so that beneficial to raising egg size.
Double benefit elements in the embodiment of the present invention include bifidobacterium adolescentis and streptomyces coelicolor.Using double benefit elements as micro- life
One of main component of state compound formulation, can effectively adjust the normal flora of egg-laying bird enteron aisle, suppress harmful bacteria growth, promote
Intestines peristalsis, strengthen immunity.
Hold, propolis can adjust the activity of a variety of enzymes, and the rheum emodin in aloe can increase the activity of lipase, the two collaboration
It can play the role of improving appetite, stomach invigorating afterwards, improve digestive function, improve laying rate；Earthworm powder and spirulina powder are good
Protein resource, can strengthen the immunity of egg-laying bird jointly with propolis, aloe, improve egg size.By adding double benefit elements, further
Mentioned component is coordinated to play the effect for adjusting intestinal health and strengthen immunity.
In addition, the embodiment of the present invention additionally provides a kind of preparation method of above-mentioned probiotics, comprise the following steps：Powder
Broken aloe, earthworm and spirulina, obtain aloe powder, earthworm powder and spirulina powder.By proportioning mixing aloe powder, earthworm powder, spiral
Algae powder and double benefit elements, obtain the first mixture, the first mixture of mixing and propolis lotion.
Optionally, aloe powder, earthworm powder, the powder particle diameter of spirulina powder can be 0.2-0.5cm, one side of the particle diameter
Face is more suitable for the feed feature of egg-laying bird, is on the other hand also beneficial to mix with propolis lotion, each component is disperseed and is mixed
It is even.
What deserves to be explained is the earthworm in the embodiment of the present invention is preferably earthworm living, before crushing, it is placed in clear water extremely
Few 3h, to remove the dirt of its inside and outside.Further, the earthworm section of 1-1.5cm can be cut into, in order to subsequently grasp
Make.
It is preferred that it will be remixed after aloe powder, earthworm powder, spirulina powder and double benefit element dryings to obtain the first mixing
Thing.Alternatively, drying can for example carry out under conditions of 60-70 DEG C, and the dry end time is with the moisture of raw material to be dried
It is criterion not higher than 3%, it is excessive to avoid each feed moisture content, it is equal that mixing is not only not easy in first time mixed process
It is even, cause the first mixture to condense caking or agglomerating, but also the mixed effect of the first mixture and propolis lotion is seriously affected,
Cause feed nutrition unbalanced.
Further, the first mixture is mixed with propolis lotion under conditions of 40-50 DEG C, under the temperature conditionss, propolis
Lotion viscosity is relatively mild, can fully be mixed with the first mixture.
What deserves to be explained is it is above-mentioned during the acquisition of the first mixture, drying temperature is arranged to 60-70 DEG C, can
Make dried raw material that still there is higher temperature, during being mixed with propolis lotion, can improve to a certain extent
The temperature of propolis lotion, makes the temperature of whole mixed system propolis lotion can be made to be in preferable flow regime just, in favor of powder
First mixture of last shape is dispersed in propolis lotion.
Alternatively, the propolis lotion in the embodiment of the present invention can for example be obtained through following steps：By propolis and polyethylene glycol
Mixing is heated under conditions of 35-40 DEG C, then adds aliphatic acid polyethenoxy ether.
Because propolis is at normal temperatures solid-state, therefore polyethylene glycol is first dissolved in the embodiment of the present invention, is more disperseed
Propolis jelly, to further improve the dissolving of propolis jelly and degree of scatter, fat is added in above-mentioned propolis jelly
Fat acid polyoxyethylene ether, the addition of the material, can destroy the chemical constitution of propolis jelly, its molecule is redistributed
And arrangement so that obtain component and property it is homogeneous and stablize propolis lotion.
Optionally, the mixed proportion of propolis, polyethylene glycol and aliphatic acid polyethenoxy ether can be 1g：20-30mL：
2-4mL.Viscosity possessed by the propolis lotion of gained is more suitable for mixing with the first mixture under the mixed proportion.
Double benefit elements can for example be obtained through following steps in the embodiment of the present invention：It is by weight 8-10:The 5-8 mixing youth is double
Discrimination bacillus and streptomyces coelicolor, obtain compound bacteria, and under ultrasound condition, compound bacteria, filtering, dried filtrate are digested with lywallzyme.
It is preferred that the weight ratio of lywallzyme and compound bacteria is 1:5-7, select lywallzyme can by bifidobacterium adolescentis with it is sky blue
The cell membrane of color streptomycete is dissolved, and it is discharged cell inclusion, so as to directly make active material and egg-laying bird
Alimentary canal contact, increase operation rate.
Through enzymolysis and dried double benefit element not only active material containing bifidobacterium adolescentis and streptomyces coelicolor,
But also functional oligosaccharide, biology enzyme and the other biological metabolic products produced at the same time containing enzymolysis, more simple strain tool
There are more benefit materials, so that with more preferably action effect.
Preferably, the viable count in the embodiment of the present invention contained by every gram of bifidobacterium adolescentis for example can be 3 × 1014-6×
1014A, the viable count contained by every gram of streptomyces coelicolor for example can be 1 × 1012-5×1012It is a.The content of above-mentioned viable count
Can substantially ensure that microbial activity material contained in double benefit elements egg-laying bird can be played more obvious raising immunity,
The effect of laying rate and egg size.
It is preferred that above-mentioned enzymolysis is that 5-6h is carried out under conditions of 26-30 DEG C, 100-120r/min, this enzymatic hydrolysis condition compared with
To be gentle, it can be ensured that be also avoided that the material inactivation of enzymolysis gained while obtaining sufficient amount of zymolyte.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Propolis is heated with polyethylene glycol under conditions of 37 DEG C and is mixed, aliphatic acid polyethenoxy ether is then added, obtains bee
Latex solution.The mixed proportion of propolis, polyethylene glycol and aliphatic acid polyethenoxy ether can be 1g：25mL：3mL.
It is 9 by weight:6.5 mixing bifidobacterium adolescentises and streptomyces coelicolor, obtain compound bacteria, in ultrasound, 28 DEG C,
Under conditions of 110r/min, compound bacteria 5.5h is digested with lywallzyme, filtering, dried filtrate, obtains double benefit elements.Lywallzyme and compound bacteria
Weight ratio be 1:6, the viable count contained by every gram of bifidobacterium adolescentis is 4.5 × 1014It is a, contained by every gram of streptomyces coelicolor
Viable count is 3 × 1012It is a.
Take earthworm living to be placed in 5h in clear water, cut into the earthworm section of 1cm.Dry aloe, earthworm, spiral shell under conditions of 65 DEG C
Revolve algae and double benefit elements are no more than 1% to its moisture, the particle diameter for being then crushed to aloe, earthworm and spirulina is
0.3cm, obtains aloe powder, earthworm powder and spirulina powder.
Mix the double of the aloe powder of 15 parts by weight, the earthworm powder of 16 parts by weight, the spirulina powder of 12 parts by weight and 8 parts by weight
Beneficial element, obtains the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 15 parts by weight and the first mixture, obtain Tiny ecosystem and answer
Close preparation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 2
Embodiment 2 and embodiment 1 difference lies in：
Mix the aloe powder of 25 parts by weight, the earthworm powder of 25 parts by weight, the spirulina powder of 24 parts by weight and 18 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 25 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 3
Embodiment 3 and embodiment 1 difference lies in：
Mix the aloe powder of 18 parts by weight, the earthworm powder of 23 parts by weight, the spirulina powder of 16 parts by weight and 14 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 18 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 4
Embodiment 4 and embodiment 1 difference lies in：
Mix the aloe powder of 22 parts by weight, the earthworm powder of 18 parts by weight, the spirulina powder of 20 parts by weight and 12 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 22 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 5
Embodiment 5 and embodiment 1 difference lies in：
Mix the aloe powder of 20 parts by weight, the earthworm powder of 20.5 parts by weight, the spirulina powder of 18 parts by weight and 13 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 20 parts by weight and the first mixture, micro- life is obtained
State compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 6
Embodiment 6 and embodiment 1 difference lies in：
Mix the aloe powder of 23 parts by weight, the earthworm powder of 20 parts by weight, the spirulina powder of 21 parts by weight and 12 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 24 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 7
Embodiment 7 and embodiment 1 difference lies in：
Mix the aloe powder of 21 parts by weight, the earthworm powder of 23 parts by weight, the spirulina powder of 22 parts by weight and 14 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 20 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 8
Embodiment 8 and embodiment 1 difference lies in：
Mix the aloe powder of 20 parts by weight, the earthworm powder of 23 parts by weight, the spirulina powder of 22 parts by weight and 17 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 45 DEG C, by the propolis lotion of 18 parts by weight and the first mixture, Tiny ecosystem is obtained
Compound formulation.
Above-mentioned probiotics is made an addition in the feed of chicken in the ratio of 1wt%.
Embodiment 9
The present embodiment and embodiment 5 difference lies in：The directly aloe powder of 20 parts by weight of disposable mixing, 20.5 parts by weight
Earthworm powder, the spirulina powder and double benefit elements of 13 parts by weight and the propolis lotions of 20 parts by weight of 18 parts by weight, obtain Tiny ecosystem
Compound formulation.
Embodiment 10
Propolis is heated with polyethylene glycol under conditions of 35 DEG C and is mixed, aliphatic acid polyethenoxy ether is then added, obtains bee
Latex solution.The mixed proportion of propolis, polyethylene glycol and aliphatic acid polyethenoxy ether can be 1g：20mL：2mL.
It is 8 by weight:5 mixing bifidobacterium adolescentises and streptomyces coelicolor, obtain compound bacteria, in ultrasound, 26 DEG C,
Under conditions of 100r/min, compound bacteria 6h is digested with lywallzyme, filtering, dried filtrate, obtains double benefit elements.Lywallzyme and compound bacteria
Weight ratio is 1:5, the viable count contained by every gram of bifidobacterium adolescentis is 3 × 1014It is a, the viable bacteria contained by every gram of streptomyces coelicolor
Number is 1 × 1012It is a.
Take earthworm living to be placed in 3h in clear water, cut into the earthworm section of 1.5cm.Under conditions of 60 DEG C dry aloe, earthworm,
Spirulina and double benefit elements are no more than 3% to its moisture, and the particle diameter for being then crushed to aloe, earthworm and spirulina is
0.2cm, obtains aloe powder, earthworm powder and spirulina powder.
Mix the aloe powder of 20 parts by weight, the earthworm powder of 20.5 parts by weight, the spirulina powder of 18 parts by weight and 13 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 40 DEG C, by the propolis lotion of 20 parts by weight and the first mixture, micro- life is obtained
State compound formulation.
Above-mentioned probiotics is made an addition in the feed of duck in the ratio of 0.5wt%.
Embodiment 11
Propolis is heated with polyethylene glycol under conditions of 40 DEG C and is mixed, aliphatic acid polyethenoxy ether is then added, obtains bee
Latex solution.The mixed proportion of propolis, polyethylene glycol and aliphatic acid polyethenoxy ether can be 1g：30mL：4mL.
It is 10 by weight:8 mixing bifidobacterium adolescentises and streptomyces coelicolor, obtain compound bacteria, in ultrasound, 30 DEG C,
Under conditions of 120r/min, compound bacteria 5h is digested with lywallzyme, filtering, dried filtrate, obtains double benefit elements.Lywallzyme and compound bacteria
Weight ratio is 1:7, the viable count contained by every gram of bifidobacterium adolescentis is 6 × 1014It is a, the viable bacteria contained by every gram of streptomyces coelicolor
Number is 5 × 1012It is a.
Take earthworm living to be placed in 8h in clear water, cut into the earthworm section of 1.3cm.Under conditions of 70 DEG C dry aloe, earthworm,
Spirulina and double benefit elements are no more than 2% to its moisture, and the particle diameter for being then crushed to aloe, earthworm and spirulina is
0.5cm, obtains aloe powder, earthworm powder and spirulina powder.
Mix the aloe powder of 20 parts by weight, the earthworm powder of 20.5 parts by weight, the spirulina powder of 18 parts by weight and 13 parts by weight
Double benefit elements, obtain the first mixture.Under conditions of 50 DEG C, by the propolis lotion of 20 parts by weight and the first mixture, micro- life is obtained
State compound formulation.
Above-mentioned probiotics is made an addition in the feed of goose in the ratio of 2wt%.
Test example 1
Repeat to implement above-described embodiment 1-11, obtain enough microorganism products.
By taking embodiment 7 as an example, approached by individual weight, the principle of health, by the Luo Manbai commodity eggs 144 of 55 week old
Plumage, is randomly divided into 3 groups (A-C groups), every group of 3 repetitions, each to repeat 16 plumages.7 days preliminary trial periods, test 28 days.Adjustment chicken group makes
Every group of difference of laying eggs is not notable.
Chicken raising is tested in open henhouse, positioned at Stall, three layers of full staged cage, 4 cage chickens are one group, per cage
Distribute 4 plumage of commodity egg.The method being combined using artificial lighting and natural lighting, daily illumination 16h, force ventilation add nature
Ventilation, daily 8:00 and 16:Once, free water, searches for food for 00 each feeding.Routinely clean and sterilize henhouse.Feeding management and
Feeding environment is consistent.The daily timing feeding of feed 2 times, daily 16：00 picks egg once.Count the egg size of each group, lay eggs
Number, inventory, remaining doses, livestock on hand chicken number, calculate average egg weight, laying rate (including damaged egg), average daily gain, material egg
Than (including damaged egg).
Test control group (A groups) feeds basal diet；Antibiotic group (B groups) feeds basal diet+antibiotic；Test group (C
Group) feeding basal diet+1wt% the embodiment of the present invention 7 microorganism products.
During experiment, following index is measured：Laying rate, day egg number, average egg weight, average daily gain and feedstuff-egg ratio.
Laying rate (%)=complete group of experimental period, lays eggs sum/(hen number × experiment number of days) × 100；
Daily ingestion amount=(dispensing total amount one remains doses)/(experiment number of days × every group of chicken number)；
Feedstuff-egg ratio=laying period feed consumption rate/total egg size.
Measured numerical value carries out one-way analysis of variance, LSD Multiple range tests using 22.0 statistical softwares of SPSS.Knot
Fruit represents with mean+SD, as shown in table 1.
Influence of 1 microorganism products of table to the production performance of laying hen
Note：Shoulder mark difference of going together capitalization represents difference extremely significantly (P<0.01), different lowercase letter indication differences are shown
Write (P<0.05), similarly hereinafter.
As can be seen from Table 1, in laying rate index, test group significantly improves (P compared with control group pole<0.01), compared with antibiosis
Plain group significantly improves (P<0.05)；In egg size index, test group dramatically increases (P compared with control group and antibiotic group pole<0.01)；
In daily ingestion amount index, test group significantly improves (P compared with control group and antibiotic group<0.05)；In feedstuff-egg ratio index, experiment
Group significantly reduces (P compared with control group pole<0.01), (P is significantly reduced compared with antibiotic group<0.05)；In broken (soft) egg rate index, examination
Test group significantly reduces (P compared with control group and antibiotic group<0.05)；In eggshell color index, the quality of test group is compared with control group
It is excellent with antibiotic group.
Also, as can be seen from Table 1, antibiotic can improve laying rate of laying hen and daily ingestion amount, reduction to a certain extent
Feedstuff-egg ratio, but effect is not notable；In addition, in egg size, broken (soft) egg rate and laying hen mortality criteria, do not play actively
Effect, reduces egg size, improves broken (soft) egg rate and the laying hen death rate on the contrary.Illustrating effect of the antibiotic to performance in layers is
Limited, or even have inhibition.
Microorganism products in test group can significantly improve laying rate, day compared with control group and antibiotic group
Feed intake and increase egg size, reduce feedstuff-egg ratio and broken (soft) egg rate, improve eggshell color.
In addition, the probiotics obtained by embodiment 1-6 and 8-11 is tested according to the method for above-mentioned test example, its
As a result same display with the addition of the egg-laying bird feed of the probiotics of the embodiment of the present invention by feeding, can compared with control group and
Antibiotic group significantly improves laying rate, daily ingestion amount and the egg size of chicken/duck/goose, reduces feedstuff-egg ratio and broken (soft) egg rate, improves egg
Shell color.
Also, in embodiment 1-8, effect possessed by embodiment 5 is optimal, illustrate that the raw material proportioning of embodiment 5 is more other
Embodiment is more preferably.Embodiment 1-8 is more excellent compared with effect possessed by embodiment 9, and it is relatively disposable mixed to illustrate that raw material mixes in batches
Close the action effect for being more advantageous to improving probiotics.
Test example 2
By taking embodiment 5 as an example, control group 1-4 is set, and wherein difference lies in be free of in raw material with embodiment 5 for control group 1
Bifidobacterium adolescentis, difference lies in streptomyces coelicolor, control group 3 and embodiment are free of in raw material with embodiment 5 for control group 2
5 difference lies in double benefit elements are free of in raw material, control group 4 is with embodiment 5 difference lies in double beneficial elements directly by youth bifid bar
Bacterium and the viable bacteria of streptomyces coelicolor are obtained by mixing.
Contrast test is carried out to embodiment 5 and control group 1-3 according to the assay method of test example 1, it is the results show that implement
Example 5 is considerably better compared with the effect that control group 1-3 plays laying hen, and the action effect trend of control group 1-3 is：2 ＞ of control group
1 ＞ control groups 3 of control group, illustrate relatively contain only wherein one with streptomyces coelicolor containing bifidobacterium adolescentis at the same time in double benefit elements
Kind has a distinct increment the production performances of laying hen without double benefit elements.
Embodiment 5 is considerably better compared with the effect that control group 4 plays laying hen, illustrates in the present invention program, by the youth
Bifidobacterium is with being made double benefit elements more directly by the work of bifidobacterium adolescentis and streptomyces coelicolor after streptomyces coelicolor enzymolysis
Bacterium mixing is more advantageous to digesting and assimilating for laying hen, so as to can more lift the production performance of laying hen.
In conclusion the microorganism products green peace provided in an embodiment of the present invention for improving laying rate and increasing egg size
Entirely, palatability is good, nutritive value is high, and dosage is few, and cost is low, effect stability.Its preparation method is simple, preparation condition controllability
It is good.Above-mentioned microorganism products are used for added in the feed of egg-laying bird, egg-laying bird growth can be promoted, strengthen it
Immunity, improve egg size and laying rate, improve breeding environment.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of microorganism products for improving laying rate and increasing egg size, it is characterised in that include the bee of 15-25 parts by weight
Latex solution, the aloe of 15-25 parts by weight, the earthworm of 16-25 parts by weight, the spirulina of 12-24 parts by weight and 8-18 parts by weight
Double benefit elements；
Double benefit elements include bifidobacterium adolescentis and streptomyces coelicolor.
2. microorganism products according to claim 1, it is characterised in that the propolis including 18-22 parts by weight
Lotion, the aloe of 18-22 parts by weight, the earthworm of 18-23 parts by weight, the spirulina and 12- of 16-20 parts by weight
Double benefit elements of 14 parts by weight.
3. microorganism products according to claim 2, it is characterised in that the propolis breast including 20 parts by weight
Liquid, the aloe of 20 parts by weight, the earthworm of 20.5 parts by weight, the institute of the spirulina of 18 parts by weight and 13 parts by weight
State double benefit elements.
4. such as the preparation method of claim 1-3 any one of them microorganism products, it is characterised in that including following step
Suddenly：The aloe, the earthworm and the spirulina are crushed, obtains aloe powder, earthworm powder and spirulina powder, institute is mixed by proportioning
Aloe powder, the earthworm powder, the spirulina powder and double benefit elements are stated, obtains the first mixture, mixing first mixing
Thing and the propolis lotion；
Preferably, the aloe powder, the earthworm powder, the powder particle diameter of the spirulina powder are 0.2-0.5cm；
Preferably, first mixture with the propolis lotion is mixed under conditions of 40-50 DEG C.
5. preparation method according to claim 4, it is characterised in that the propolis lotion is obtained through following steps：By bee
Glue is heated under conditions of 35-40 DEG C with polyethylene glycol and mixed, and then adds aliphatic acid polyethenoxy ether；
The propolis, the mixed proportion of the polyethylene glycol and the aliphatic acid polyethenoxy ether are 1g：20-30mL：2-4mL.
6. preparation method according to claim 4, it is characterised in that double benefit elements are obtained through following steps：By weight
Than for 8-10:5-8 mixing bifidobacterium adolescentis and streptomyces coelicolor, obtain compound bacteria, under ultrasound condition, are digested with lywallzyme
The compound bacteria, filtering, dried filtrate；
The weight ratio of the lywallzyme and the compound bacteria is 1:5-7；
Preferably, the viable count contained by every gram of bifidobacterium adolescentis is 3 × 1014-6×1014It is a, every gram of sky blue chain
Viable count contained by mould is 1 × 1012-5×1012It is a.
7. preparation method according to claim 5, it is characterised in that enzymolysis is in the bar of 26-30 DEG C, 100-120r/min
5-6h is carried out under part.
8. preparation method according to claim 5, it is characterised in that by the aloe powder, earthworm powder, described spiral
Algae powder and double benefit elements are dried to moisture under conditions of 60-70 DEG C and are not higher than 3%, then remix to obtain described
First mixture.
9. such as the application of claim 1-3 any one of them microorganism products, it is characterised in that the Tiny ecosystem is compound
Preparation is used for added in the feed of egg-laying bird；
Preferably, the egg-laying bird includes any one in chicken, duck and goose.
10. the application of microorganism products according to claim 9, it is characterised in that the microorganism products
Made an addition in the ratio of 0.5-2wt% in the feed of the egg-laying bird.
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