CN106036185A - Laying hen feed additive - Google Patents
Laying hen feed additive Download PDFInfo
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- CN106036185A CN106036185A CN201610608568.0A CN201610608568A CN106036185A CN 106036185 A CN106036185 A CN 106036185A CN 201610608568 A CN201610608568 A CN 201610608568A CN 106036185 A CN106036185 A CN 106036185A
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Abstract
The invention belongs to the biotechnology field, and particularly relates to the agriculture field of biotechnology and poultry farming, in particular to a laying hen feed additive. The laying hen feed additive is prepared from the following ingredients in parts by weight: 0.1-0.8 percent of polypeptide, 15-28 percent of mycose, 0.2-1.2 percent of recombinant inactive enterotoxin B sigmasubunit, 5-10 percent of glycerinum, 14-34 percent of milk powder, 2-6 percent of malt dry powder and 15-28 percent of probiotics powder. All components of the additive are evenly mixed and are evenly mixed with laying hen complete feed at the ratio of 0.2-0.5% to feed the laying hen. The laying hen feed additive to which the invention relates can dramatically improve the immunocompetence of the laying hen and obviously reduces laying hen morbidity and death rates. Meanwhile, the laying hen feed additive can regulate the internal secretion and the nutrition metabolism of the laying hen, increases the immunoglobulin level in eggs and obviously improves the quality of eggs.
Description
Technical field
The invention belongs to biological technical field, especially biotechnology cultivates agriculture field with fowl, is specially a kind of laying hen
Feed additive.
Background technology
Along with the development of intensive culture industry, breeding layer chicken scale is increasing, and almost all of layer breeding enterprise is all
Use mixed feed.Due to the raw material of life-time service homogeneity, changing laying hen and take food multiformity, the local flavor of produced egg declines,
Coarse mouthfeel, nutritive value reduces.High density intensive culture makes breeding environment ecosystem structure single, laying hen resistance
Weak, cross infection easily occur and spreads on a large scale, bringing tremendous economic to lose to raiser.In order to tackle various disease, support
Grow family to have to use chemical drugs and antibiotic in a large number, but these medicines not only change the normal group of laying hen intestinal microbial population
Becoming, in intestinal, microecological environment imbalance, also makes medicine remain in vivo, and the health hampering nuisanceless breeding layer chicken industry is sent out
Exhibition.It addition, along with the raising of people's living standard and the reinforcement of health perception, healthy value of livestock product be there has also been by people
New demand.Exploitation and the novel livestock products of productive consumption person's demand, improve the surcharge of product, to raising with high-tech
Population quality and culture benefit are respectively provided with significance.It should be noted that in egg, the value of immunoglobulin is gradually by people
Study and pay attention to.In egg, immunoglobulin has higher heat stability and specificity, such as the immune globulin in lacto
As Bai, in egg, immunoglobulin can effectively press down and kills harmful levels of pathogens, the growth of protection Embryo Gallus domesticus；Immunity in we take in egg
During globulin, it also can press down and kills pernicious bacteria in gastrointestinal, thus human intestines and stomach plays effective protective effect.
The nutrition of polypeptide and regulatory function, have been obtained for extensive concern and application in recent years.Having of having occurred in that is anti-
The raw element class medicine containing polypeptide, such as polymyxins, bacitracin and vancomycin, its structure is as follows:
The molecular structure of colistin sulfate
Bacillus subtilis bacitracin
But above antibiotics polypeptide is often complicated multichained construction, and usually contains glycosyl or other group, has anti-
Raw element activity.
Summary of the invention
The present invention is just for the deficiencies in the prior art and defect, it is provided that a kind of feed addictive of layer chicken.This adds
Add the simple in construction of polypeptide in agent, only common protein Single polypeptide chain, without other structure or groups such as glycosyls, do not detect
Having antibacterial activity, do not possess the characteristic of antibiotic, functional polypeptide and probiotic powder as feed additive, can be shown by the application
Write regulation laying hen enteric microorganism ecological balance, improve the immunocompetence of laying hen and reduce diarrhoea, improving simultaneously as nutrition
Egg quality and reduce fishy smell, has obvious novelty, and safe and reliable, and can large-scale application in actual production.
The concrete technical scheme of the present invention is as follows:
A kind of feed addictive of layer chicken, including the material of following percentage composition: active polypeptide 0.1-0.8%, trehalose 10-
28%, recombinate inactive enterotoxin subunit B 0.2-1.2% (Sigma, article No. S4881), glycerol 5-10%, milk powder 14-
34%, Fructus Hordei Germinatus dry powder 2-6%, 15-28% probiotic powder, gross mass percentage composition is 100%.
Described active polypeptide is any one or the mixing of three peptide species in 3 peptide species, and three peptide species mix with matter
Amount ratio is 1:1:1.In described 3, the aminoacid sequence composition of polypeptide is following (left: polypeptide N-end；Right: polypeptide-C end):
NO.1, LRPDPKRTIQKKSE, comprise 14 amino acid residues；
NO.2, GAYSNQSIKASIG, comprise 13 amino acid residues；
NO.3, LGFFGKGNSSNE, comprise 12 amino acid residues.
The synthesis of above-mentioned 3 peptide species uses prior art to synthesize, and desalination, purity requirement reaches more than 95%.
The component of described probiotic bacteria and mass percent be: 10-30% bacillus bifidus powder, 30-50% bacillus subtilis
Powder and 40-60% Bacillus licheniformis powder, gross mass percentage composition is 100%.
Active Bifidobacterium dry powder is mixed with corresponding culture medium, forms bacillus bifidus powder, in terms of weight/mass percentage composition, institute
Stating the active Bifidobacterium containing 90-95% in bacillus bifidus powder, surplus is its relevant culture medium.
Bacillus subtilis dry powder is mixed with corresponding culture medium, forms bacillus subtilis mycopowder, with weight/mass percentage composition
Meter, contains the bacillus subtilis viable bacteria of 12-20% in described bacillus subtilis, surplus is its relevant culture medium.
Bacillus licheniformis dry powder is mixed with corresponding culture medium, forms Bacillus licheniformis powder, contain with percent mass
Gauge, contains the Bacillus licheniformis viable bacteria of 30% in described Bacillus licheniformis powder, surplus is its relevant culture medium.
The method preparing the above feed addictive of layer chicken, comprises the following steps: weigh in proportion polypeptide, trehalose,
Recombinate inactive enterotoxin subunit B, glycerol, milk powder, the probiotic composition of Fructus Hordei Germinatus dry powder sum, then carried out mixing all
Even, it is the additive of egg feedstuff.Feed additive, can store for 2 years in the dry environment of 4 DEG C.
With weight/mass percentage composition i.e., feed addictive of layer chicken is mixed with egg feedstuff according to 0.2-0.5%, then
Direct-fed laying hen.Described laying hen can be the blue ash in sea.
The inactive enterotoxin subunit B of restructuring employed in the application is commercially available prod, such as can use product man be
Sigma, article No. is the commercially available prod of S4881.
The positive effect of the present invention is embodied in:
(1), the present invention is new strengthening laying hen immunity and improve the feed additive of egg quality.
(2), as food, nourishing additive agent and immunomodulator, the function of polypeptide is closed the most widely
Note.Mostly current polypeptide products is to obtain by decomposing plant animal protein, and the polypeptide that the present invention relates to is to close from artificial
In the peptide library become, 3 polypeptide finally determined by screening, it is totally different from the polypeptide products developed.
(3), our early-stage Study show that this polypeptide is arbitrary or combination of two with probiotic bacteria by percent mass proportioning as egg
Chicken additive, is all remarkably improved the immunity of laying hen and the taste of egg product.This invention be a kind of new safety, cost can accept
Improve laying hen immunity and the feed additive of egg quality.
(4), this feed addictive of layer chicken is remarkably improved the immunocompetence of laying hen, hence it is evident that reduce laying hen morbidity with dead
Die rate；Meanwhile, feed additive scalable laying hen endocrine of the present invention and Nutrition and Metabolism, increase immune globulin plain boiled water in egg
Flat, significantly improve the quality of egg.
Detailed description of the invention:
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with detailed description of the invention pair
The present invention is described in further detail, but this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to following enforcement
Example.Raw material in following example is commercially available prod.
Embodiment 1:
1. synthesis NO.1 polypeptide
According to the aminoacid sequence of NO.1 polypeptide, transfer to Shanghai Rong Xi biological engineering company limited to synthesize NO.1 polypeptide, need
Desalting processing and purification, it is desirable to purity reaches more than 95%.The aminoacid sequence of NO.1 polypeptide is as follows:
SEQ ID NO.1:LRPDPKRTIQKKSE, comprises 14 amino acid residues；
2. the preparation of probiotic powder
In terms of weight/mass percentage composition, the active Bifidobacterium dry powder by 90% and the culture medium mixing of 10%, form bifid
Bacillus mycopowder.Wherein culture medium prescription (dry solid material is in terms of 100wt%): peptone 13.74%, yeast extract powder
10.9%, Carnis Bovis seu Bubali cream 7.0%, Tomato juice 25.6%, lactose 1.5%, glucose 22.7%, anhydrous sodium acetate 8.9%, seven water
Conjunction magnesium sulfate 1.2%, Manganous sulfate monohydrate 0.26%, diammonium hydrogen citrate 2.5%, potassium dihydrogen phosphate 2.5%, tween 2.5%,
L-cysteine hydrochloride 0.7%, culture medium prescription gross mass percentage composition is 100%.
In terms of weight/mass percentage composition, 20% bacillus subtilis dry powder and 80% culture medium are mixed, forms hay spore
Bacillus mycopowder.Wherein culture medium prescription (dry solid material): glucose 64.4%, peptone 15.4%, yeast extract 9.2%, phosphorus
Acid dihydride potassium 6.4% and calcium carbonate 4.6%, culture medium prescription gross mass percentage composition is 100%.
In terms of weight/mass percentage composition, the Bacillus licheniformis dry powder by 30% and the mixing of 70% culture medium, form lichens bud
Spore bacillus mycopowder.Wherein culture medium prescription (dry solid material): brown sugar 0.7%, Testa oryzae 42%, wheat bran 57.3%, cultivates basigamy
Side's gross mass percentage composition is 100%.
Further, the mycopowder of above-mentioned 3 kinds of bacterium is sufficiently mixed, forms probiotic composition, with weight/mass percentage composition
Meter, its mixed proportion is: 30% Bifidobacteria powder, 30% bacillus subtilis mycopowder and 40% Bacillus licheniformis powder.
3. the preparation of feed addictive of layer chicken
According to mass percent, by polypeptide 0.4%, trehalose 28%, restructuring enterotoxin subunit B 0.2%, glycerol 6%,
Milk powder 34%, the probiotic composition of Fructus Hordei Germinatus dry powder 6% and 25.4%, mix, be the additive of egg feedstuff.Feedstuff adds
Add agent, can store for 2 years in the dry environment of 4 DEG C.
Feed addictive of layer chicken is mixed with commercially available full price feed for layer chicken according to 0.3% mass percent, the most directly
Connect and feed laying hen.
Embodiment 2:
1. synthesis NO.2 polypeptide
According to the aminoacid sequence of NO.2 polypeptide, transfer to Shanghai Rong Xi biological engineering company limited to synthesize NO.2 polypeptide, need
Desalting processing and purification, it is desirable to purity reaches more than 95%.The aminoacid sequence of NO.2 polypeptide is as follows:
SEQ ID NO.2:SEQ ID NO.2:GAYSNQSIKASIG, comprises 13 amino acid residues；
2. the preparation of probiotic powder
In terms of weight/mass percentage composition, the active Bifidobacterium dry powder of 90% and the culture medium of 10% are mixed, formed
Bifidobacteria powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: peptone
13.74%, yeast extract powder 10.9%, Carnis Bovis seu Bubali cream 7.0%, Tomato juice 25.6%, lactose 1.5%, glucose 22.7%, nothing
Water sodium acetate 8.9%, bitter salt 1.2%, Manganous sulfate monohydrate 0.26%, diammonium hydrogen citrate 2.5%, biphosphate
Potassium 2.5%, tween 2.5%, L-cysteine hydrochloride 0.7%.
In terms of weight/mass percentage composition, the bacillus subtilis dry powder of 20% and the culture medium of 80% are mixed, formed
Bacillus subtilis mycopowder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: glucose
64.4%, peptone 15.4%, yeast extract 9.2%, potassium dihydrogen phosphate 6.4% and calcium carbonate 4.6%.
In terms of weight/mass percentage composition, the Bacillus licheniformis dry powder of 30% and the culture medium of 70% are mixed, formed
Bacillus licheniformis powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: brown sugar
0.7%, Testa oryzae 42%, wheat bran 57.3%.
Further, the mycopowder of above-mentioned 3 kinds of bacterium is sufficiently mixed in proportion, forms probiotic composition, its mass ratio
For: 20% Bifidobacteria powder, 30% bacillus subtilis mycopowder and 50% Bacillus licheniformis powder.
3. the preparation of feed addictive of layer chicken
According to mass percent, by polypeptide 0.4%, trehalose 28%, restructuring enterotoxin subunit B 0.2%, glycerol 6%,
Milk powder 34%, the probiotic composition of Fructus Hordei Germinatus dry powder 6% and 25.4%, mix, be the additive of egg feedstuff.Feedstuff adds
Add agent, can store for 2 years in the dry environment of 4 DEG C.
Feed addictive of layer chicken is mixed with full price feed for layer chicken according to the mass percent of 0.3%, the most directly
Feed laying hen.
Embodiment 3:
1. synthesis NO.3 polypeptide
According to the aminoacid sequence of NO.3 polypeptide, transfer to Shanghai Rong Xi biological engineering company limited to synthesize NO.3 polypeptide, need
Desalting processing and purification, it is desirable to purity reaches more than 95%.The aminoacid sequence of NO.3 polypeptide is as follows:
SEQ ID NO.3:APVSNGGTKTET, comprises 12 amino acid residues；
2. the preparation of probiotic powder
In terms of weight/mass percentage composition, the active Bifidobacterium dry powder of 90% and the culture medium of 10% are mixed, formed
Bifidobacteria powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: peptone
13.74%, yeast extract powder 10.9%, Carnis Bovis seu Bubali cream 7.0%, Tomato juice 25.6%, lactose 1.5%, glucose 22.7%, nothing
Water sodium acetate 8.9%, bitter salt 1.2%, Manganous sulfate monohydrate 0.26%, diammonium hydrogen citrate 2.5%, biphosphate
Potassium 2.5%, tween 2.5%, L-cysteine hydrochloride 0.7%.
In terms of weight/mass percentage composition, the bacillus subtilis dry powder of 20% and the culture medium of 80% are mixed, formed
Bacillus subtilis mycopowder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: glucose
64.4%, peptone 15.4%, yeast extract 9.2%, potassium dihydrogen phosphate 6.4% and calcium carbonate 4.6%.
In terms of weight/mass percentage composition, the Bacillus licheniformis dry powder of 30% and the culture medium of 70% are mixed, formed
Bacillus licheniformis powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: brown sugar
0.7%, Testa oryzae 42%, wheat bran 57.3%.
Further, the mycopowder of above-mentioned 3 kinds of bacterium is sufficiently mixed in proportion, forms probiotic composition, its mass ratio
For: 20% Bifidobacteria powder, 30% bacillus subtilis mycopowder and 50% Bacillus licheniformis powder.
3. the preparation of feed addictive of layer chicken
According to mass percent, by polypeptide 0.4%, trehalose 28%, restructuring enterotoxin subunit B 0.2%, glycerol 6%,
Milk powder 34%, the probiotic composition of Fructus Hordei Germinatus dry powder 6% and 25.4%, mix, be the additive of egg feedstuff.Feedstuff adds
Add agent, can store for 2 years in the dry environment of 4 DEG C.
Feed addictive of layer chicken is mixed with full price feed for layer chicken according to 0.4% mass percent, the most directly raises
Feed laying hen.
Embodiment 4:
1. synthesis polypeptide
Respectively according to the aminoacid sequence of 3 peptide species, transfer to Shanghai Rong Xi biological engineering company limited to synthesize polypeptide, need to take off
Salt treatment and purification, it is desirable to purity reaches more than 95%.The aminoacid sequence of NO.3 polypeptide is as follows:
SEQ ID NO.1:LRPDPKRTIQKKSE, comprises 14 amino acid residues；
SEQ ID NO.2:GAYSNQSIKASIG, comprises 13 amino acid residues；
SEQ ID NO.3:APVSNGGTKTET, comprises 12 amino acid residues；
Finally, 3 peptide species are mixed according to mass ratio 1:1:1 ratio, form polypeptides in combination powder.
2. the preparation of probiotic powder
In terms of weight/mass percentage composition, the active Bifidobacterium dry powder of 90% and the culture medium of 10% are mixed, formed
Bifidobacteria powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: peptone
13.74%, yeast extract powder 10.9%, Carnis Bovis seu Bubali cream 7.0%, Tomato juice 25.6%, lactose 1.5%, glucose 22.7%, nothing
Water sodium acetate 8.9%, bitter salt 1.2%, Manganous sulfate monohydrate 0.26%, diammonium hydrogen citrate 2.5%, biphosphate
Potassium 2.5%, tween 2.5%, L-cysteine hydrochloride 0.7%.
In terms of weight/mass percentage composition, the bacillus subtilis dry powder of 20% and the culture medium of 80% are mixed, formed
Bacillus subtilis mycopowder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: glucose
64.4%, peptone 15.4%, yeast extract 9.2%, potassium dihydrogen phosphate 6.4% and calcium carbonate 4.6%.
In terms of weight/mass percentage composition, the Bacillus licheniformis dry powder of 30% and the culture medium of 70% are mixed, formed
Bacillus licheniformis powder.Wherein culture medium prescription is (dry solid material) in terms of 100% by gross mass percentage composition: brown sugar
0.7%, Testa oryzae 42%, wheat bran 57.3%
Further, the mycopowder of above-mentioned 3 kinds of bacterium is sufficiently mixed in proportion, forms probiotic composition, its mass ratio
For: 20% Bifidobacteria powder, 30% bacillus subtilis mycopowder and 50% Bacillus licheniformis powder.
3. the preparation of feed addictive of layer chicken
According to mass percent, by polypeptide 0.4%, trehalose 28%, restructuring enterotoxin subunit B 0.2%, glycerol 6%,
Milk powder 34%, the probiotic composition of Fructus Hordei Germinatus dry powder 6% and 25.4%, mix, be the additive of egg feedstuff.Feedstuff adds
Add agent, can store for 2 years in the dry environment of 4 DEG C.
Experimental example
1, animal experiment design
Test and Selection 30 week old sea orchid ash laying hen 600, is randomly divided into 6 test group, often group 100, respectively A, B,
C, D, E, F group, laboratory observation totally 100 days.Test chicken is all raised at same hen house, and chicken house usual manner is pressed in immunity and feeding and management
Carry out.Day feeds 2 times, material foot, freely drinks water, longitudinal ventilation fan, illumination every day 16 hours, illumination every cubic metre 3～4 watts.Often
It scrapes excrement 1 time.
2, test feed
By the feed addictive of layer chicken of above-mentioned case study on implementation 1-4 according to 0.5% mass percent and full price feed for layer chicken (city
Sell laying hen normal feedstuff) mix, then Direct-fed laying hen.Specifically it is grouped as follows:
A group: conventional full price feed for layer chicken, for the conventional base feedstuff of brown shell layer.
B group: the full price feed for layer chicken+0.5% feed addictive of layer chicken without polypeptide.
C group: the feed addictive of layer chicken described in embodiment 1 of full price feed for layer chicken+0.5%.
D group: the feed addictive of layer chicken described in embodiment 2 of full price feed for layer chicken+0.5%.
E group: the feed addictive of layer chicken described in embodiment 3 of full price feed for layer chicken+0.5%.
F group: the feed addictive of layer chicken described in embodiment 4 of full price feed for layer chicken+0.5%.
3, index determining
Record laying hen has loose bowels situation, egg outward appearance, egg number, laying rate, food consumption and breakage, soft egg number etc..Tool
Body result of the test see table 1:
Table 1 feed addictive of layer chicken is on brown shell layer growth and the impact of egg laying performance
| Mensuration project | A group | B group | C group | D group | E group | F group |
| Laying rate (%) | 87.2 | 90.2 | 90.1 | 89.5 | 91.4 | 90.7 |
| Feedstuff-egg ratio (g/g) | 2.19 | 2.14 | 2.10 | 2.23 | 2.18 | 2.11 |
| Rate of broken eggs (%) | 2.51 | 2.27 | 1.88 | 2.13 | 2.02 | 1.81 |
| Egg fishy smell (opens shell) | +++++ | ++++ | +++ | ++++ | +++ | +++ |
| Laying hen intestinal diarrhea rate (%) | 31 | 21 | 16 | 19 | 14 | 11 |
| Laying hen death or mortality (%) | 8 | 5 | 4 | 5 | 4 | 3 |
Remarks: laying hen intestinal diarrhea rate, refer to that dysfunction occurs in laying hen intestinal during observing, and show diarrhoea or draw
Dilute and feces is sugar chicken droppings shape.
Claims (6)
1. a feed addictive of layer chicken, it is characterised in that include the material of following percentage composition: active polypeptide 0.1-0.8%, sea
Algae sugar 10-28%, the inactive enterotoxin subunit B 0.2-1.2% that recombinates, glycerol 5-10%, milk powder 14-34%, Fructus Hordei Germinatus dry powder 2-6%,
15-28% probiotic powder, gross mass percentage composition is 100%.
A kind of feed addictive of layer chicken the most according to claim 1, it is characterised in that: described active polypeptide is more than 3 kinds
Any one or the mixing of three peptide species in peptide, three peptide species mix with mass ratio as 1:1:1, and aminoacid sequence forms such as
Under:
NO.1, LRPDPKRTIQKKSE；
NO.2, GAYSNQSIKASIG；
NO.3, LGFFGKGNSSNE.
A kind of feed addictive of layer chicken the most according to claim 1, it is characterised in that the component of described probiotic bacteria and quality
Percent is: 10-30% bacillus bifidus powder, 30-50% bacillus subtilis mycopowder and 40-60% Bacillus licheniformis powder, gross mass hundred
Dividing content is 100%.
A kind of feed addictive of layer chicken the most according to claim 3, it is characterised in that: in terms of weight/mass percentage composition, described
Containing the active Bifidobacterium of 90-95% in bacillus bifidus powder, surplus is its relevant culture medium.
A kind of feed addictive of layer chicken the most according to claim 3, it is characterised in that: in terms of weight/mass percentage composition, described
Containing the bacillus subtilis viable bacteria of 12-20% in bacillus subtilis, surplus is its relevant culture medium.
A kind of feed addictive of layer chicken the most according to claim 3, it is characterised in that: in terms of weight/mass percentage composition, described
Containing the Bacillus licheniformis viable bacteria of 30% in Bacillus licheniformis powder, surplus is its relevant culture medium.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610608568.0A CN106036185A (en) | 2016-07-28 | 2016-07-28 | Laying hen feed additive |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610608568.0A CN106036185A (en) | 2016-07-28 | 2016-07-28 | Laying hen feed additive |
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| CN106036185A true CN106036185A (en) | 2016-10-26 |
Family
ID=57195802
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| CN201610608568.0A Pending CN106036185A (en) | 2016-07-28 | 2016-07-28 | Laying hen feed additive |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110754582A (en) * | 2019-11-27 | 2020-02-07 | 贵州宏扬饲料有限公司 | Chicken feed and processing technology thereof |
| CN113207798A (en) * | 2020-01-21 | 2021-08-06 | 泰安合生世纪生物科技有限公司 | Laying hen feed, method for increasing astaxanthin content of eggs and functional eggs |
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| CN113207798A (en) * | 2020-01-21 | 2021-08-06 | 泰安合生世纪生物科技有限公司 | Laying hen feed, method for increasing astaxanthin content of eggs and functional eggs |
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