The specific embodiment
Embodiment 1, prepare 1% green compound premix for laying period of layers, prepare Hangzhou Minshen Bio-Tech Co., Ltd. and produce, (the special-purpose multivitamin of laying cycle of laying hens, main composition is vitamin A, D to the special-purpose multidimensional of the laying hen of model 513
3, E, K, B
1, B
2, B
6, B
12, niacinamide, folic acid, biotin, calcium pantothenate etc.) account for 3kg, the precious 200(yeast of the refined health viable count that the refined fine jade bio tech ltd in methionine 15kg, the Guangzhou that methionine content is 98.5% is produced>=20,000,000,000/gram) phytase 0.7kg, n-propyl gallate (antioxidant) 0.05kg, Choline Chloride 5kg that Choline Chloride content is 50%, betaine 5kg that beet alkali hydrochlorate content is 98% raise in 6kg, sodium bicarbonate 15kg, Xinhua, with excellent long element, (every kilogram contains lactic acid bacteria>=1.0 * 10 to the fowl that Chongqing Unibio Biological Technology Co., Ltd. produces
12cFU, bacillus>=1.51 * 10
13cFU, yeast cell wall polysaccharide>=8%,) 2kg, for fowl complex enzyme (main composition is zytase, 1,4 beta-glucanase, cellulase, protease, amylase etc., Wuhan Xinhuayang Biological Co., Ltd produces, production code member 512DA) 1kg, Fe content are not less than 30% ferrous fumarate 3.24kg, Zn content is not less than 34.5% ZnSO
4h
2o 3kg, Mn content are not less than 31.8% MnSO
4h
2o 2.5kg, Cu content are not less than 25% CuSO
45H
2o 0.4kg, be not less than 2% CaIO containing I amount
3h2O 0.3kg, Raman selenium (Se content is not less than 0.2% yeast selenium) 1kg, zeolite powder 25kg, powdered rice hulls 7kg for fowl;
Above-mentioned raw materials is pulverized and all analyzed sieve by 1.18mm successively, and 0.60mm analyzes sieve, and oversize must not be greater than 10%, and accurately weighing mixes, and mixing should be even, and its coefficient of variation (CV) should be not more than 5%.
Get the above-mentioned premix mixed and mix according to weight ratio 1:99 with energy material (corn etc.), albumen raw material, mineral raw material, for the feeding laying hen.
Toxicity acute experiment:
1, experiment purpose: 1% laying hen compound premixed feed carries out the acute toxicity test of its mouse oral gastric infusion.
2, experiment material: 1% laying hen compound premixed feed (code name: 5410), proterties be canescence to the lark powder, deposit in the tested material storeroom, be placed in sealing in drier, normal temperature is preserved.
3, exploiting entity: Yunnan Province natural drug pharmacology key lab
4, animal used as test title, germline, grade:
SPF level ICR mouse derives from unming Medical College's Experimental Animal Center, the animal used as test production licence number: SCXK(Yunnan) 2005-0008, occupancy permit number: SYXK(Yunnan) 2005-0003, issuing unit: Yunnan Province science and technology bureau.Sex, age, quantity and weight range are in Table 1.
Table 1 animal situation list
, experimental technique
5.1 animal grouping: by the body weight segmentation, by the method for drawing lots at random, be divided into two groups after weighing respectively by 30 animal male and female, that is: the solvent control group is 10,20 of administration groups, male and female half and half.Male and female are divided cage, 5, every cage.
5.2 animals recognition method: draw circle sign group on the mouse tail with the black permanent pen, a circle means the solvent control group, and two circles mean the administration group.Represented units with saturated picric acid by wool dyeing, represent tens to cut auris dextra, cut left ear and represent two tens, female tom is identified respectively, is numbered.Duration of test is looked by hair color fading situation repeating label at any time.Respectively solvent control group and administration group cage box being hung to cage card sign indicates: special topic numbering, thematic director, cage number, animal number, sex and test the beginning and ending time.Each is organized the cage card and beats the √ mark with the different colours marking pen in the cage card lower left corner, and the solvent control group is with yellow, and the administration group is identified by redness.
5.3 the dosage design considerations: tested material belongs to feed addictive, mainly is comprised of Multiple components such as amino acid, trace element, vitamin A, vitamin D, vitamin E and B family vitamins.Because the tested material ingredient is mainly organism desired nutritional element, therefore with reference to GB 15193.1-2003 " toxicological evaluation of food safety procedure " and Zheng Jianxian chief editor, " functional food " the 3rd volume that China Light Industry Press publishes, the test specification of " safe toxicology test technology ".This test is carried out mtd test with the ICR mouse.
5.4 tested material preparation: take tested material 5.0g and be placed in beaker on the AEG220 electronic analytical balance, add after appropriate solvent stirs, mixes and add solvent to 20 ml, be mixed with the suspension that concentration is 25%.
5.5 animal fasting: the front animal fasting of weighing be can't help water 12~14 hours.
5.6 administration capacity: 40ml/kg.bw.
5.7 the approach of giving and number of times: consistent with the use approach, employing waits capacity per os gastric infusion 1 time.
5.8 experimental observation
5.8.1 ordinary circumstance and sign: observe animal appearance sign, behavioral activity, mental status, face, genitals and ight soil proterties etc., Continuous Observation 14 days during administration and after administration.
5.8.2 changes of weight: respectively at test, claim the weight of animals once, observed and recorded changes of weight situation in 0 day, 3 days, 7 days, 10 days, 14 days.
5.8.3 toxic reaction and death: whether the toxic reaction symptom of observed and recorded animal, initial and duration, toxic reaction be reversible, death time of animal, quantity etc.
5.8.4 system is dissected and is visually observed: during off-test, animal is carried out to the system dissection, visually observe in splanchnocoel whether abnormal hydrops is arranged, check shape size, color, the quality of each organs and tissues.Internal organs and tissue surface have or not the abnormal conditions such as hemostasis, hemorrhage, tubercle, ulcer, abscess, adhesion, necrosis and lump.
5.9 statistical method: adopt SPSSwin12.0 software with paired t-test, the weight of animals being carried out to statistical analysis in groups.
6, result
6.1 ordinary circumstance and sign: after gavage gives tested material, freely, feed, drinking-water are normal, fur gloss, the state of mind are good, Continuous Observation 14 days, all animals survival for animal activity.
6.2 changes of weight: duration of test day part two treated animal body weight all increase, comparison there was no significant difference between group (
p0.05), before and after test relatively, body weight have significant difference (
p<0.01).In Table 2,3
Table 2 1% laying hen compound premixed feed on the impact of female mouse body weight (
± S)
* and comparison in 0 day, have significant difference
p<0.01; Compare there was no significant difference between group
p0.05
Table 3 1% laying hen compound premixed feed on the impact of male mouse body weight (
± S)
* and comparison in 0 day, have significant difference
p<0.01; Compare there was no significant difference between group
p0.05
6.3 system is dissected and to be visually observed: all survival test animals are carried out to gross anatomy and visually observe, the heart, liver, spleen, lung, kidney main organs, organ quality, color and luster are normal, without abnormal changes such as enlargement, adhesion, hyperemia.
Conclusion
1% laying hen compound premixed feed carries out the acute toxicity test of ICR mouse stomach, and result shows: within after gavage continuous 14 days, observe, weight of mice, do not find overt toxicity reaction and dead.1% laying hen compound premixed feed mouse stomach LD
5010.0g/40ml/kg.bw.According to acute toxicity (LD
50) the classification 1% true border of laying hen compound premixed feed innocuous substance.
Experimental result is explained
The acute oral toxicity result is one of important parameter of evaluating chemical thing acute toxicity.LD
50result only shows toxicity character and the intensity of tested material, can not be as unique foundation of judging its security.Therefore should, as an absolute index identifying tested material toxicity, only not supply further toxicology test reference.The reliability that is extrapolated to the different genera animal and human from results of animal is limited.
The effect test:
Test unit: animal science and technology institute of Yunnan Prov Agriculture University
1 materials and methods
1.1 experimental animal and test grouping
6000 of the 105 blue brown commodity eggs in ages in days sea of choosing that body weight is close, healthy, appetite is good, be divided into 4 groups at random, wherein establishes 3 test group (I, II, III group) and 1 control group.Establish 3 repetitions for every group, each repeats 500 chickens.Respectively organize laying hen during grouping and just started to lay eggs, regularity is better.
1.2 test site and time
Test site is carried out at cloud Kunming Xin Kang people's animal husbandry Co., Ltd commodity egg chicken house, on June 20, in time from 5 days to 2011 July in 2010.Experimental period is 50 weeks.
1.3 test diet and feeding and management
Test daily ration composition and trophic level are in Table 4.
Test chicken adopts five layers of staged of totally-enclosed house to raise in cages, 4, every cage.It is identical that each organizes feeding and management condition, machinery location feeding, nipple-shape drinking-water, longitudinal ventilation.Illumination adopts natural lighting to add artificial light, keeps illumination every day 16 hours.Regularly clear excrement, ventilation.Every day, 10:30 and 15:00 manually received egg.Other management and immune more solito are carried out.
1.4 test index and method
1.4.1 the mensuration of performance in layers
Egg production respectively organized in record weekly, measures laying rate, draws the laying rate change curve;
Take and repeat to measure average egg production, average egg weight, average daily ingestion amount, egg material ratio as unit;
When on-test and off-test, the laying hen individuality is weighed, and measures the weightening finish of laying hen average body.
1.4.2 the mensuration of the average survival rate of laying hen, average rate of broken eggs, average lopsided egg rate
Record the death toll of every group of each repetition laying hen, calculate average survival rate;
Record total broken egg amount and total egg production of every group of each repetition, calculate average rate of broken eggs;
Record total lopsided egg amount and total egg production of every group of each repetition, calculate average lopsided egg rate.
Table 4 daily ration composition and trophic level (air bells dry basis, %)
1.4.3 the mensuration of egg quality
Respectively at test the 1st, 5,9,13,17,21,25,29,33,37,41,45,49 weeks, every group each repeated to randomly draw 5 pieces of eggs, measures quality the statistics of egg.
1. measure average shell thickness: measure with the shell thickness analyzer.
2. measure average eggshell density: the method for pressing Toylor is measured.
3. measure average egg shape index.
4. measure average yolk, albumen water content and protein content.
1.5 data are processed
Test data adopts 10.0 editions statistical softwares of SPSS to carry out variance analysis and multiple ratio.
2 results and analysis
2.1 the impact of different daily rations on performance in layers
2.1.1 laying rate and laying rate change
Different daily rations on the impact of laying rate in Table 5, table 6, the laying rate situation of change is shown in.Each test group laying rate of laying hen ascendant trend is fast than control group, and later stage laying rate downward trend is slow than control group; Each test group of egg-laying peak is all held time for a long time than control group, and the laying rate of each test group egg-laying peak is all high than control group; The average egg production of each test group laying hen is compared with control group, all increases, and the average egg production of test group I, II, III group has improved 6.13%, 5.81%, 5.85% than control group respectively, but different disposal group difference not significantly (P>0.05).
The impact of the different diets of table 5 on laying rate of laying hen
Unit: %
The different diet average egg productions of table 6
Group | Average egg production (%) |
Test I group | 75.96±0.03 |
Test II group | 75.73±0.02 |
Test III group | 75.76±0.02 |
Control group | 71.57±0.02 |
The average egg weight of the different diets of table 7, feed intake, feedstuff-egg ratio and body weight gains
Group | Average egg weight (g) | Average daily ingestion amount (g/d) | Average feedstuff-egg ratio | Average body weightening finish (g) |
The test group I | 56.84±1.01 | 106.41±0.17 | (2.58±0.17)︰1 | 620±1.22 |
The test group II | 57.14±0.89 | 107.28±0.29 | (2.60±0.24)︰1 | 613±0.86 |
The test group III | 56.67±1.28 | 106.53±0.61 | (2.57±0.35)︰1 | 621±0.51 |
Control group | 55.47±1.02 | 106.95±0.37 | (2.70±0.12)︰1 | 590±0.73 |
2.2 the impact of different diets on the average survival rate of laying hen, rate of broken eggs, lopsided egg rate
Different diets on the impact of the average survival rate of laying hen, rate of broken eggs, lopsided egg rate in Table 8.As known from Table 8, average laying hen survival rate is the highest with the test group III, and minimum is control group, the statistical variance analysis, and each group difference is remarkable (P>0.05) not; Average rate of broken eggs is the highest with control group, and minimum is the test group II, and each group difference is remarkable (P>0.05) not; Average lopsided egg rate is the highest with control group, and the test group II is minimum, and each group difference is remarkable (P>0.05) not.
The impact of the different diets of table 8 on the average laying hen survival rate of laying hen, rate of broken eggs, lopsided egg rate
Group | Laying hen survival rate (%) | Rate of broken eggs (%) | Deformity egg rate (%) |
Test group I | 95.4±0.57 | 4.389±0.024 | 4.382±0.027 |
Test group II | 95.7±0.23 | 4.327±0.029 | 3.974±0.019 |
Test group III | 96.1±0.45 | 4.482±0.016 | 4.272±0.013 |
Control group | 94.2±0.55 | 5.824±0.024 | 4.972±0.074 |
2.3 the impact of different diets on egg quality
Different diets on the impact of egg quality in Table 9.Analytical table 9 is known, and average shell thickness is the highest with the test group III, and minimum be control group, but each group difference not remarkable (P>0.05); Eggshell density is the highest with test group III, and control group is minimum, and each group difference is remarkable (P>0.05) not; Egg shape index is the highest with test group II, and the test group III is minimum, and each group difference is remarkable (P>0.05) not.
The impact of the different diets of table 9 on egg quality
Group | Shell thickness (mm) | Eggshell density (mg/cm
3)
| Egg shape index |
Test group I | 0.372±0.022 | 92.4±2.7 | 0.754±0.007 |
Test group II | 0.392±0.031 | 91.7±6.4 | 0.765±0.024 |
Test group III | 0.407±0.016 | 92.9±6.5 | 0.747±0.017 |
Control group | 0.371±0.024 | 90.4±5.5 | 0.755±0.024 |
2.4 the impact of different diets on the egg main chemical compositions
Different diets on the impact of egg main chemical compositions in Table 10.By table 10, can be obtained, average yolk water content is the highest with the test group III, and minimum be control group, but each group difference not remarkable (P>0.05); Average egg white water content is the highest with control group, and test group I is minimum, and each group difference is remarkable (P>0.05) not; Average egg yolk protein content is the highest with test group II, and control group is minimum, and each group difference is remarkable (P>0.05) not.Average albumen protein content is the highest with the test group III, and control group is minimum, and each group difference is remarkable (P>0.05) not.
The impact of the different diets of table 10 on the egg chemical composition
Unit: %
Group | The yolk water content | The egg white water content | Egg yolk protein content | Albumen protein content |
Test group I | 45.9±1.5 | 85.3±1.2 | 16.1±0.2 | 12.4±0.2 |
The test group II | 45.8±1.3 | 86.2±1.4 | 16.2±0.3 | 12.5±0.1 |
The test group III | 46.1±1.1 | 86.7±1.6 | 16.1±0.7 | 12.7±0.5 |
Control group | 45.2±1.2 | 87.4±1.3 | 15.7±0.8 | 12.3±0.2 |
3 discuss
In poultry feed, can utilize phosphorus to should be 0.40~0.60%, and can only provide 0.1~0.2% utilized phosphorus in plant feed.For meeting in the animal body growth and development process the demand of phosphorus, usually in feed, add 1~3% high phosphorus feedstuff.So far still mainly use the raw materials such as calcium monohydrogen phosphate, bone meal.But the quality problems of calcium monohydrogen phosphate are more at present, are mainly the harmful element content overproofs such as fluorine, lead, arsenic, easily cause the poultry depauperation, and store in vivo, cause body poisoning, death.These harmful elements are discharged contaminated environment with ight soil, enter human body by approach such as grain, drinking-water again, damage.And also there are potential safety hazard in various degree in the restriction of the raw material sources of bone meal and sanitary index problem, some countries have forbidden that homology animal protein raw material is for animal and fowl fodder for this reason.Studies have shown that, the high phosphorus pollution on the environment is just more and more endangered and is being coerced the human lives.Pig fowl is limited in one's ability to utilizing of phytate phosphorus, in feed, unemployed phosphorus is directly excreted, enter water system with liquid dung, rainwater, the eutrophication that causes high phosphorus etc. in water system, make water plant, microbiological anomaly growth, ecological balance heavy damage in water, cause water pollution to go bad, foul, the water resource severe exacerbation, disruption of ecological balance etc.In recent years the red tide in lake, reservoir, coastal waters, red tide frequency and area are more and more higher, are exactly illustration.This is the Tough questions that the animal husbandry production development faces.
Simultaneously laying hen opens that Postpartum Diets forms and whether a day trophic level is rationally that the feeding quality quality is directly connected to the performance of production performance of layer chicken and the quality of egg quality, relation people's food security and the economic benefit of laying hen industry, can be described as the key point that poultry is produced, therefore must give enough attention.
Add the utilization rate that phytase improves phytate phosphorus in feed, thereby reduce the consumption reduction Feed Manufacturing cost of the phosphorus source forages such as calcium monohydrogen phosphate, bone meal, significantly reduce the pollution of phosphorus to environment, alleviate so that thoroughly stop fluorine poisoning of calcium monohydrogen phosphate etc.This result of the test shows, in the laying hen diet, adds phytase, can reduce by 50% left and right of calcium monohydrogen phosphate use amount, in conjunction with the use of probiotic, can obtain preferably laying hen production achievement.Lateral comparison, can make local poultry dealer obtain higher economic benefit, again can be from protection of the environment, and the angle of sustainable development, filter out the high-quality feed that meets local breeding environment.
Compare every performance in layers index and egg quality index between three test group and control group, the visible difference all not significantly (P>0.05) of respectively organizing, but the production performance index of three test group and egg quality are all a little more than control group, explanation is in this test under identical feeding and management condition, add phytase in the diet of three test group, reduce the consumption of Phos in feed formula simultaneously, can improve equally the biological effectiveness of phosphorus in egg feedstuff.Explanation again, the interpolation of phytase can bring economic benefit to production, can alleviate the immense pressure that the livestock and poultry breeding industry high phosphorus causes environment again, fundamentally protection of the environment sustainable development.
In addition, protein allowance is the principal element that affects laying rate and egg quality, and suitable protein level can obtain higher and lasting laying rate, egg size and protein content.In this test, the average egg production of three test group, average egg weight and protein content are all a little more than control group, in comparatively desirable state, the needs that three test group Different Dietary Protein Levels can meet the laying hen growth fully and produce are described, and can obtain larger economic well-being of workers and staff.
Quality of egg inferior is to cause laying hen one of the major reason of loss of already helping always, and the Major Nutrient that affects quality of egg have calcium, phosphorus, sodium, potassium, zinc, manganese, VD and VC etc.Calcium is the main component of eggshell, and in feed, during calcium deficiency, the thickness of eggshell and density reduce, so in the feed of laying hen, sufficient calcium should be arranged.Phosphorus be animal the essential and a kind of mineral element in close relations with the metabolism of calcium, in daily ration the phosphorus level not enough or with calcium in nonequilibrium condition, can reduce laying hen egg yield and eggshell quality, high levels of phosphorus is unfavorable for the absorption of calcium, also can reduce eggshell quality.Sodium, potassium, zinc, manganese, VD and VC reasonably can significantly improve quality of egg under scope.The shell thickness of three test group, density index are all than more excellent, and eggshell quality, in higher level, illustrate that the match ratio of calcium, phosphorus, sodium, potassium, zinc, manganese, VD and VC in three test group daily rations is more reasonable, and quality is higher.
4 conclusions
Comprehensive above result of the test can draw to draw a conclusion: 1. under this experimental condition, three kinds of mixed feeds of " Bang Ge " 5,410 1% laying hen compound premix preparations and the laying hen mixed feed of certain company are fed after laying hen, all can obtain performance in layers and egg quality index preferably, and difference not significantly (P>0.05).2. under this experimental condition, three kinds of batches of Yunnan " Bang Ge " 5,410 1% laying hen compound premixs preparations are fed after laying hen, and the production performance of laying hen and egg quality all are better than the laying hen mixed feed of certain company.3. under this experimental condition, the interpolation of phytase in egg feedstuff can reduce use amount and bring economic benefit to the producer, can alleviate the immense pressure that the livestock and poultry breeding industry high phosphorus causes environment again, the sustainable development of fundamentally preserving the ecological environment.4. three kinds of batch Major Nutrients of the 5410 1% laying hen compound premixs preparations of Yunnan " Bang Ge " and the match ratios such as trace element and vitamin are more reasonable, and quality is higher, and feeding effect is obvious, is worth of widely use.