CN111184140A - Feed additive for improving production performance of laying quails - Google Patents
Feed additive for improving production performance of laying quails Download PDFInfo
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- CN111184140A CN111184140A CN202010055070.2A CN202010055070A CN111184140A CN 111184140 A CN111184140 A CN 111184140A CN 202010055070 A CN202010055070 A CN 202010055070A CN 111184140 A CN111184140 A CN 111184140A
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- 241000286209 Phasianidae Species 0.000 title claims abstract description 59
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 28
- 239000003674 animal food additive Substances 0.000 title claims abstract description 26
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 24
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 17
- 241000709785 Hermetia illucens Species 0.000 claims abstract description 17
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims abstract description 17
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the field of cultivation, and discloses a feed additive for improving the production performance of laying quails, which comprises the following substances in parts by weight: 0.01-0.05 part of xylooligosaccharide, 0.06-0.1 part of mannooligosaccharide, 1-3 parts of bacillus subtilis, 1-3 parts of bacillus licheniformis, 0.5-2 parts of compound lactic acid bacteria and 10-15 parts of hermetia illucens dry insect powder; according to the invention, oligosaccharide substances with specific molecular weights are selected, and some compound lactic acid bacteria, bacillus subtilis and bacillus licheniformis are additionally added in vitro, so that beneficial bacteria can be fixedly planted in animal intestinal tracts, the number of the beneficial bacteria in the animal intestinal tracts is obviously increased, and the immunity of the laying quails is favorably improved. The feed additive is added into basic ration of the laying quails, so that the production performance of the laying quails can be obviously improved, and the average egg weight is improved by 0.26%; the laying rate is improved by 4.99 percent; the death and elutriation rate of ten thousand weeks is reduced by 20.66 percent.
Description
Technical Field
The invention relates to the technical field of cultivation, and particularly relates to a composition for improving the production performance of laying quails and application thereof.
Background
Quail (Coturnix) is small and round, has dark and dark feathers, and is usually slightly different between male and female. The small brown color band has obvious straw yellow spear stripes and irregular stripes, and the male and female bodies have reddish brown and black transverse stripes. The male chin is dark brown, and the midline of the larynx bends upwards to the ear pinna and is tightly attached to the yellowish necklace. The yellowish eyebrows contrast significantly with the brown tops of the heads and the lines of the eyes. Female birds also had similar patterns but the control was not obvious.
Quail eggs are considered as 'ginseng in animals' and are suitable for being eaten as nourishing dietary therapy products frequently, and are called 'good in egg' because of having unique nutrition. The egg quail is the main part of laying quail eggs, and the prior art is less to the research of the production performance of egg quail, leads to egg quail production performance relatively poor, can't satisfy social demand.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the problems in the prior art and provides a feed additive for improving the production performance of laying quails.
A second object of the invention is to provide the use of the above feed supplement.
The purpose of the invention is realized by the following technical scheme:
a feed additive for improving the production performance of laying quails comprises the following substances in parts by weight: 0.01-0.05 part of xylooligosaccharide, 0.06-0.1 part of mannooligosaccharide, 1-3 parts of bacillus subtilis, 1-3 parts of bacillus licheniformis, 0.5-2 parts of compound lactic acid bacteria and 10-15 parts of hermetia illucens dry insect powder.
In the feed additive, the content of the black soldier fly body protein is high, the black soldier fly body protein can be used as an excellent source for animal protein supplement, and a plurality of antibacterial peptides can be generated in the black soldier fly body, so that the immunity of the laying quail is synergistically enhanced with oligosaccharides such as xylooligosaccharide and mannooligosaccharide; in addition, the oligosaccharide substances can improve the quantity of beneficial bacteria in animal intestinal tracts, but the effect is not obvious, and the invention selects the oligosaccharide substances with specific molecular weights and additionally adds some compound lactic acid bacteria, bacillus subtilis and bacillus licheniformis in vitro, so that the beneficial bacteria can be planted in the animal intestinal tracts, the quantity of the beneficial bacteria in the animal intestinal tracts is obviously improved, and the invention is beneficial to improving the immunity of the laying quails.
Preferably, in the feed additive, the bacterial load of each part of bacillus subtilis is 1.5-3 multiplied by 1012cfu; the bacterial amount of each part of the bacillus licheniformis is 0.5-1 multiplied by 1010cfu; the compound lactobacillus is selected from two or three of lactobacillus casei, lactobacillus plantarum and bifidobacterium, wherein the lactobacillus casei, the lactobacillus plantarum, the bifidobacterium and the like are mixed in mass, and the viable count of the lactobacillus casei, the lactobacillus plantarum and the bifidobacterium is 1.0 multiplied by 108cfu/g~1.5×109cfu/g。
Preferably, the molecular weight of the xylo-oligosaccharide is 1500-2000, and the molecular weight of the manno-oligosaccharide is 1500-2000 Da. The research of the invention finds that the regulation effect of oligosaccharide substances on intestinal microflora is related to the types and molecular mass of oligosaccharides, and the invention can ensure that the flora in the intestinal tract of the laying quail is in an environment where beneficial bacteria are really advantageous by selecting xylo-oligosaccharide and mannooligosaccharide with specific molecular weight, namely, the quantity and the types of the beneficial bacteria in the intestinal tract are increased to the maximum extent, thereby improving the immunoregulation capability of the laying quail.
More preferably, the feed additive for improving the production performance of the laying quails comprises the following substances in parts by weight: 0.01 part of xylooligosaccharide, 0.04 part of mannooligosaccharide, 2 parts of bacillus subtilis, 2 parts of bacillus licheniformis, 1 part of compound lactic acid bacteria and 14.95 parts of black soldier fly dry insect powder.
The invention also improves the application of the feed additive in improving the production performance of the laying quails. When the feed additive is added into basic ration of egg-laying quails, the ability of resisting the environment in the growth process of the egg-laying quails can be obviously improved, the immunity of the egg-laying quails is improved, and meanwhile, the production performance of the egg-laying quails can also be improved.
Preferably, the feed additive is used for improving the production performance of the laying quails, wherein the production performance comprises average egg weight, number of stockings, material-egg ratio and death rate. The application of the feed additive can obviously improve the production performance of laying quails.
Specifically, the feed additive is applied to improving the production performance of the laying quails, and is added into the basic ration of the laying quails. More specifically, the addition amount is 3-8% of the mass of the basic ration.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a feed additive for improving the production performance of laying quails, which comprises the following substances in parts by weight: 0.01-0.05 part of xylooligosaccharide, 0.06-0.1 part of mannooligosaccharide, 1-3 parts of bacillus subtilis, 1-3 parts of bacillus licheniformis, 0.5-2 parts of compound lactic acid bacteria and 10-15 parts of hermetia illucens dry insect powder; the black soldier fly has high protein content and can be used as an excellent source for animal protein supplement, and a plurality of antibacterial peptides can be generated in the black soldier fly, so that the black soldier fly and oligosaccharides such as xylo-oligosaccharide and mannooligosaccharide can synergistically enhance the immunity of the laying quails; in addition, the oligosaccharide substances can improve the quantity of beneficial bacteria in animal intestinal tracts, but the effect is not obvious, and the invention selects the oligosaccharide substances with specific molecular weights and additionally adds some compound lactic acid bacteria, bacillus subtilis and bacillus licheniformis in vitro, so that the beneficial bacteria can be planted in the animal intestinal tracts, the quantity of the beneficial bacteria in the animal intestinal tracts is obviously improved, and the invention is beneficial to improving the immunity of the laying quails. The feed additive is added into basic ration of the laying quails, so that the production performance of the laying quails can be obviously improved, and the average egg weight is improved by 0.26%; the laying rate is improved by 4.99 percent; the death and elutriation rate of ten thousand weeks is reduced by 20.66 percent.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
In the following examples, the basic ration for laying quails comprises the following substances in percentage by mass: 50% of corn, 13.8% of bran, 10.4% of soybean meal, 8.1% of rice bran, 9.0% of rapeseed cake, 1.5% of fish meal, 6.9% of stone powder and 0.3% of salt. The black soldier fly dry insect powder is obtained by grinding commercially available black soldier fly dry powder; the compound lactobacillus is a commercial product.
Example 1
The feed additive for improving the production performance of the laying quails comprises the following substances in parts by weight: 0.01 part of xylooligosaccharide, 0.04 part of mannooligosaccharide, 2 parts of bacillus subtilis, 2 parts of bacillus licheniformis, 1 part of compound lactic acid bacteria and 14.95 parts of black soldier fly dry insect powder.
Wherein the molecular weight of xylooligosaccharide is 1500, the molecular weight of mannooligosaccharide is 1500Da, and the bacterial load of Bacillus subtilis is 1.5 × 1012cfu; the bacterial amount of each part of Bacillus licheniformis is 0.5 × 1010cfu. The composite lactobacillus is lactobacillus plantarum and bifidobacterium according to the mass ratio of 1: 1, mixing; the viable count of the lactobacillus plantarum and the bifidobacterium is 1.2 multiplied by 10 respectively8cfu/g。
Example 2
The feed additive for improving the production performance of the laying quails comprises the following substances in parts by weight: 0.03 part of xylooligosaccharide, 0.08 part of mannooligosaccharide, 1 part of bacillus subtilis, 1 part of bacillus licheniformis, 0.5 part of compound lactic acid bacteria and 12.39 parts of black soldier fly dry insect powder.
Wherein the molecular weight of xylooligosaccharide is 1800, the molecular weight of mannooligosaccharide is 1800Da, and the bacterial load of each part of Bacillus subtilis is 2 × 1012cfu; the bacterial amount of each part of Bacillus licheniformis is 0.8 × 1010cfu. The composite lactobacillus is lactobacillus casei and lactobacillus plantarum, and the mass ratio of lactobacillus casei to lactobacillus plantarum is 1: 1, mixing; the viable count of the lactobacillus plantarum and the lactobacillus casei is respectively1.0×108cfu/g。
Example 3
The feed additive for improving the production performance of the laying quails comprises the following substances in parts by weight: 0.05 part of xylooligosaccharide, 0.1 part of mannooligosaccharide, 3 parts of bacillus subtilis, 3 parts of bacillus licheniformis, 2 parts of compound lactic acid bacteria and 11.85 parts of black soldier fly dry insect powder.
Wherein the molecular weight of xylooligosaccharide is 2000, the molecular weight of mannooligosaccharide is 2000Da, and the bacterial load of Bacillus subtilis is 3 × 1012cfu; the bacterial amount of each part of the bacillus licheniformis is 1 multiplied by 1010cfu. The composite lactobacillus is lactobacillus casei, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1: 1: 1, mixing; the viable count of the lactobacillus plantarum, the bifidobacterium and the lactobacillus casei is respectively 1.5 multiplied by 109cfu/g。
Experimental example 1
Experiment time: year 2019, day 10, month 8 to day 11, month 25, location: a quail farm in the Jinhua City Jindong district.
Experiment of quail: self-matched yellow feather male and female lines capable of laying eggs for 1 month.
The experimental method comprises the following steps: 49000 feather-laying quails in the same house are randomly divided into a 25000 feather test group and a 24000 feather control group, the test group adds the feed additives of the above examples 1 to 3 into basic daily ration, the addition amount is 5 percent of the mass of the basic daily ration, and the control group feeds the basic daily ration. The pre-test period is 10 days, the trial period is 49 days, and the feed consumption, the egg production, the feed-egg ratio, the egg production rate and the death and culling rate of each group of the ten thousand feathers per week and the feed-egg ratio, the death and culling rate and the profit of each group of the whole period are calculated by taking the week as a unit. Conventional breeding management is adopted, and each group with diseases is treated according to symptoms. Data were statistically analyzed using SPSS software and data are presented as "mean ± standard deviation".
The results of the experiment are shown in table 1, and the data in table 1 were analyzed: the feed additive of the group of the embodiment is added into basic ration, the full-period feed-egg ratio and the week feed-egg ratio of the laying quail are respectively reduced by 3.45%, 3.94% and 3.45%, and the requirement of reducing the feed-egg ratio by 3% in the project contract index is met. The weekly mortality rate of the example groups is reduced by more than 15%, the full-period mortality rate is reduced by more than 10%, and the requirements that the project index mortality rate is reduced by more than 10% are met. The full-season ten-thousand-feather profit of the embodiment group is improved by about 15 percent, and the requirement that the project index is higher than 10 percent is met.
In the experimental period, the control group has 2 times of medication, the cost is 420 yuan, the costs of the examples 1 to 3 are respectively 170 yuan, 180 yuan and 150 yuan, because the antibiotics are forbidden to the egg-laying poultry, the Chinese herbal medicine preparations are adopted, the cost of the medicines of the examples 1 to 3 is respectively reduced by 61.9 percent, 63.09 percent and 64.3 percent compared with the control group, and the requirement of reducing the contract requirement by more than 60 percent is achieved. The weight of defective eggs in the example group is reduced by 33.37 percent, 34.55 percent and 40 percent respectively compared with the control group, which indicates that the eggs in the control group have more fallopian tube diseases than the control group.
TABLE 1
Experimental example 2
Selecting the egg quails fed in the examples 1 to 3 and the control group, taking 0.1g of cecal content under aseptic condition, washing the cecal content clean with sterile normal saline, then sucking the cecal content dry with filter paper, placing the cecal content in a sterilized glass homogenizer filled with glass beads, adding a small amount of sterile normal saline (0.85% NaCl) for homogenizing, fixing the volume to 1mL after homogenizing, performing gradient dilution by taking the homogenized sample as a stock solution, selecting the dilution degree of bacterial colonies, respectively coating 0.1mL of the cecal content on a BS culture medium (selected bifidobacterium), an LBS culture medium (selected lactobacillus), an EMB culture medium (selected enterobacteria) and EC enterococcus (selected enterococcus), coating 3 plates on each group, performing inverted culture at 28 ℃ for 24 hours, and calculating the average bacterial colony number (X) of the same dilution degree by using a colony counter. CFU/g samples were calculated using the CFU formula, and the calculation results were expressed in logarithmic form (logN/g), and the results are shown in Table 2. The CFU calculation formula is as follows:
CFU/g specimen ═ X/0.1 × dilution factor × 1/specimen weight (g).
TABLE 2
As can be seen from Table 2, the number of bifidobacteria and lactobacilli in intestinal tracts of the laying quails of the example group is remarkably increased, which shows that the feed additive provided by the invention can regulate the structure of intestinal flora of the laying quails, improve intestinal microenvironment, improve the immunity of the laying quails, and also remarkably improve the capability of the laying quails in digesting and absorbing nutrient substances in the feed, thereby improving the production performance of the laying quails.
Experimental example 3
The egg quails fed in examples 1 to 3 and the control group were selected, 10 eggs were randomly selected from each group, weighed on empty stomach, slaughtered according to a conventional method, bursa and spleen were taken and weighed, and immune organ index was calculated. The results are shown in table 3, and it can be seen from table 3 that the feed supplement of the present invention can increase spleen and bursa of fabricius of laying quails, indicating that it can significantly enhance immune status of laying quails.
Immune organ index is the wet weight of immune organ (mg)/body weight (g).
TABLE 3
Group of | Spleen index | French bladder index |
Example 1 | 2.510±0.231 | 2.599±0.214 |
Example 2 | 2.515±0.213 | 2.601±0.209 |
Example 3 | 2.586±0.234 | 2.676±0.222 |
Control group | 1.759±0.218 | 2.513±0.214 |
Claims (7)
1. The feed additive for improving the production performance of laying quails is characterized by comprising the following substances in parts by weight: 0.01-0.05 part of xylooligosaccharide, 0.06-0.1 part of mannooligosaccharide, 1-3 parts of bacillus subtilis, 1-3 parts of bacillus licheniformis, 0.5-2 parts of compound lactic acid bacteria and 10-15 parts of hermetia illucens dry insect powder.
2. The feed additive for improving the productivity of laying quails according to claim 1, wherein the bacterial amount of bacillus subtilis per part is 1.5-3 x 1012cfu。
3. The feed additive for improving the production performance of laying quails according to claim 2, wherein the molecular weight of the xylo-oligosaccharide is 1500-2000, and the molecular weight of the mannan-oligosaccharide is 1500-2000 Da.
4. The feed additive for improving the productivity of laying quails according to claim 3, which comprises the following substances in parts by weight: 0.01 part of xylooligosaccharide, 0.04 part of mannooligosaccharide, 2 parts of bacillus subtilis, 2 parts of bacillus licheniformis, 1 part of compound lactic acid bacteria and 14.95 parts of black soldier fly dry insect powder.
5. Use of the feed supplement according to any of claims 1 to 4 for improving the productivity of laying quails.
6. Use of a feed supplement according to claim 5 for improving the production performance of laying quails, wherein the production performance includes average egg weight, number of stockpiles, feed-egg ratio and mortality.
7. Use of a feed supplement according to claim 6 for improving the productivity of egg quails, wherein the feed supplement is added to the basal ration of egg quails.
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Cited By (3)
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CN113142418A (en) * | 2021-05-07 | 2021-07-23 | 焦作美博士饲料有限公司 | Compound fermented quail feed |
CN114468171A (en) * | 2020-10-23 | 2022-05-13 | 河北科星药业有限公司 | Feed additive for preventing and treating quail coccidiosis, preparation method and application |
RU2816010C1 (en) * | 2023-07-25 | 2024-03-25 | федеральное государственное бюджетное образовательное учреждение высшего образования "Белгородский государственный технологический университет им. В.Г. Шухова" | Method for producing probiotic with cuticle of pupae of black soldier fly for preventing and treating avian dysbacteriosis |
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CN110192540A (en) * | 2019-07-09 | 2019-09-03 | 山东农业大学 | A kind of test method that the dry worm of black soldier flies influences performance in layers and Egg Quality |
CN110463856A (en) * | 2019-09-05 | 2019-11-19 | 广东驱动力生物科技股份有限公司 | A kind of feed addictive improving Layer Production Performance |
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CN107898815A (en) * | 2017-12-07 | 2018-04-13 | 生物源生物技术(深圳)股份有限公司 | A kind of animal inactivation lactobacillus preparation and application thereof |
CN110192540A (en) * | 2019-07-09 | 2019-09-03 | 山东农业大学 | A kind of test method that the dry worm of black soldier flies influences performance in layers and Egg Quality |
CN110463856A (en) * | 2019-09-05 | 2019-11-19 | 广东驱动力生物科技股份有限公司 | A kind of feed addictive improving Layer Production Performance |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114468171A (en) * | 2020-10-23 | 2022-05-13 | 河北科星药业有限公司 | Feed additive for preventing and treating quail coccidiosis, preparation method and application |
CN113142418A (en) * | 2021-05-07 | 2021-07-23 | 焦作美博士饲料有限公司 | Compound fermented quail feed |
CN113142418B (en) * | 2021-05-07 | 2023-03-14 | 焦作美博士饲料有限公司 | Compound fermented quail feed |
RU2816010C1 (en) * | 2023-07-25 | 2024-03-25 | федеральное государственное бюджетное образовательное учреждение высшего образования "Белгородский государственный технологический университет им. В.Г. Шухова" | Method for producing probiotic with cuticle of pupae of black soldier fly for preventing and treating avian dysbacteriosis |
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