CN107879842A - A kind of preparation method of orchard composite biological organic fertilizer - Google Patents
A kind of preparation method of orchard composite biological organic fertilizer Download PDFInfo
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- CN107879842A CN107879842A CN201711140680.7A CN201711140680A CN107879842A CN 107879842 A CN107879842 A CN 107879842A CN 201711140680 A CN201711140680 A CN 201711140680A CN 107879842 A CN107879842 A CN 107879842A
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- organic fertilizer
- charcoal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Abstract
The invention belongs to agricultural biological organic fertilizer field, disclose a kind of preparation method of orchard composite biological organic fertilizer, include the preparation of straw biological charcoal, fatten in charcoal field, rhizobium leguminosarum and the culture and preparation of gel-shaped series bacillus high efficiency composition microbial inoculum, the screening of leguminous green manure plant, the preparation processes such as the nature of resigning from office and returning home of leguminous green manure plant is decomposed, inoculation is with field adaptive faculty and the rhizobium leguminosarum of competitiveness and gel-shaped series bacillus in legume, green manure plant earthing is converted into high-quality biological organic fertilizer after resigning from office and returning home, straw biomass charcoal, complex microorganism and the coupling of leguminous green manure plant, abundant nutriment and organic matter can be provided for soil.Organic fertilizer prepared by the present invention can improve the structure composition of soil, improve liquid manure gas and coordinate to supply, raising land resources utilization rate, the conversion and utilization of reinforced soil organic fertilizer, N P and K, improve the yield and quality of fruit.
Description
Technical field
The present invention relates to technical field of biological fertilizer, specifically a kind of preparation side of orchard composite biological organic fertilizer
Method.
Background technology
Being excessively used for chemical fertilizer has been the bottleneck problem for influenceing China's agricultural sustainable development, reduces fertilizer amount to China's agriculture
Industry production, food security and environmental protection are significant, and biological organic fertilizer is green as the chemical fertilizer substitute of fat-reducing synergy
The trend of color agricultural development, and realize the inevitable approach of " arriving the year two thousand twenty chemical fertilizer zero growth rate ".Tianshui city is NORTHWEST CHINA
The main fruit producing region in area, fruit industry production are the special advantage industries of the sky and water, and the pillar industry of regional economy.In recent years by
Become increasingly conspicuous the problem of the excessive input of chemical fertilizer causes fruit quality to decline, pest and disease damage increases, and it is this area mountain that arid is barren
The principal character of ground orchard soil, it is distinct issues the most that the content of organic matter is too low, seriously governs the life of high-quality fruit
Production.
Charcoal has special anatomical structure and physicochemical property, in soil remediation, lifting soil fertility and promotes plant growth
Etc. there is obvious effect, agriculture and forestry organic waste material charing returning to the field is improved the soil and will provide new way for China's agricultural sustainable development.
Using charcoal as base manure, the rhizobium of the drought-resistant colleges and universities of screening salt resistance alkali combine pulse family with the colloid bacillus cereus of phosphorus decomposing, potassium decomposing
Green manure plant is adapted to organic fertilizer supply model for the barren hillside orchard structure of arid.
The content of the invention
It is an object of the invention to provide a kind of preparation method of orchard biological organic fertilizer, to solve this area due to chemical fertilizer
The problem of excessive input causes fruit quality to decline, pest and disease damage increases.
To achieve the above object, the technical solution used in the present invention is：
A kind of preparation method of orchard composite biological organic fertilizer, the orchard composite biological organic fertilizer include straw biological charcoal, pulse family
Green manure, rhizobium leguminosarum and gel-shaped series bacillus, it is characterised in that：It comprises the following steps：
Step 1: the preparation of straw biological charcoal：To originate in the maize straw of the Northwest as raw material, using retort in temperature
For 400 DEG C, cornstalk biological charcoal is prepared in stove under conditions of oxygen percent by volume 5%, organic carbon in prepared charcoal
Mass percentage content be 50%, the mass percentage content of ash content is 20%；
Step 2: charcoal field soil is cultivated：By the charcoal prepared by step 1 with 200g/m2Dosage October throw
Enter in hillside orchard soil, earthing thickness 10cm, decomposed six months under nature；
Step 3: rhizobium leguminosarum and gel-shaped series bacillus strain multiple cropping：Pea root is taken respectively with model 5ul oese
Knurl bacterium and the oese of gel-shaped series bacillus one enter to be configured to strain suspension in 10ml distilled water, take rhizobium leguminosarum strain
Suspension 50ul is inoculated in root nodule bacterium culture medium with rubbing method, is placed in constant incubator, is cultivated 3 days for 30 DEG C in temperature；Take glue
Freeze sample series bacillus strain suspension 50ul streak inoculations in silicate bacteria selective medium, be placed in constant incubator
In, 30 DEG C of temperature is cultivated 5 days；
Step 4: the expansion culture of strain：The oese of rhizobium leguminosarum single bacterium colony one brought back to life after cultivating is taken to add with 5ul oeses
Enter 10ml distilled water and be configured to strain suspension, take rhizobium leguminosarum strain suspension 80ul to be inoculated in the training of liquid root nodule bacteria liquid
Base 100ml is supported, is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 3 days, cultivation temperature is 30 DEG C；Will be multiple
Gel-shaped series bacillus single bacterium colony after culture living takes an oese to add 10ml distilled water and is configured to strain with 5ul oeses
Suspension, gel-shaped series bacillus strain suspension 80ul is taken to be inoculated in silicate bacteria selected liq culture medium 100ml,
It is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 5 days, cultivation temperature is 30 DEG C；
Step 5: the preparation of composite bacteria agent：The thalline cultivated using rotational tubby centrifuge collection step four is weighed, with pea root
Knurl bacterium strain quality, which accounts for 50%, gel-shaped series bacillus strain quality and accounts for 44%, honey 4%, wheat bran 2%, is prepared into mixing thalline,
Emulsified with homogenizer, freeze dryer be put into after emulsification, under vacuum conditions, -40 DEG C of snap frozens, block thalline is taken out after lyophilized,
Powder is broken into food masher, with serial dilutions spread plate, calculates viable count；Using the charcoal prepared by step 1 as
Adhesive, it is 1 by the thalli powder smashed and charcoal mass ratio:10 ratio prepares graininess microbial inoculum；
Step 6: the screening of leguminous green manure plant：Composite bacteria agent prepared by step 5 is inoculated in common vetch, big pigeon ash
Pea, white pea or French beans legume, efficient syntaxial system is built for strain, and select the optimal green manure pulse family of effect of inoculation
Plant；
Step 7: the nature of resigning from office and returning home of leguminous green manure plant is decomposed：By the composite bacteria agent prepared by step 5 with 200g/ before and after grain rains
m2The optimal green manure seeds of leguminous plant mixed seeding that is screened of dosage and step 5 fattened in step 2 straw biological charcoal
The field of six months, Routine Management produces 5 months, naturally decomposed by legume herb overburden soil table after harvesting, and orchard is made
The mass percentage content of composite biological organic fertilizer, wherein organic matter is 18.5%-25.7%, the mass percentage content of organic carbon
Mass percentage content for 22.3%-27.1%, N P and K is 27.4%-35.5% or 7.4%-10.3% or 8.6%-13.6%.
Preferably, the rhizobium culture medium prescription：Dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, sodium chloride 0.2g, sucrose
Or mannitol 10.0g, yeast extract 4.0g, mass percent concentration are 0.5% sodium molybdate solution 4.0ml, mass percent concentration
For 0.5% boric acid solution 4.0ml, calcium carbonate 5.0g, agar 18.0g, the mL mixtures of distilled water 1000, root nodule bacterium culture medium
PH value is 7.2-7.4.
Preferably, the silicate bacteria selective medium formula：Glucose 5.0g, magnesium sulfate 0.5g, calcium carbonate
0.1g, the g of disodium hydrogen phosphate 2.0, iron chloride, 0.005g, glass dust 1.0g, the g of agar 15.0, the mL of distilled water 1000 mixing
Thing, the pH value of silicate bacteria selective medium is 7.0.
The rhizobium leguminosarum（Rhizobium leguminosarum）With gel-shaped series bacillus
（Paenibacillusmucilaginosus）Purchase in China General Microbiological culture presevation administrative center, rhizobium leguminosarum
Deposit number is：Rl1.2327, gel-shaped series bacillus deposit number are 1.153.
The invention provides a kind of Organic fertilizer production method for hillside orchard high-quality Fruits production, with pea root nodule
Bacterium（Rhizobium leguminosarum）With gel-shaped series bacillus（Paenibacillusmucilaginosus）It is double to connect
Plant in leguminous green manure plant, then high-quality biological organic fertilizer is converted into after green manure plant earthing is resigned from office and return home, straw biomass charcoal, answer
The coupling of microorganism and leguminous green manure plant is closed, abundant nutriment and organic matter can be provided for soil, soil knot can be improved
Structure forms, and coordinates soil, water, fertilizer, gas integral, cost is low, efficiency high, is a kind of composite biomass organic fertilizer of safe and high quality
Production method.The present invention makes full use of limited land resource, by agricultural wastes rational utilization, in environmental protection section
Provided safeguard while about agricultural cost is put into for the production of green environmentally friendly high-quality Organic Fruit.
Embodiment
The present invention is further detailed explanation below.
A kind of preparation method of orchard composite biological organic fertilizer, the orchard composite biological organic fertilizer include straw biological charcoal,
Leguminous green manure, rhizobium leguminosarum and gel-shaped series bacillus, it is characterised in that：It comprises the following steps：
Step 1: the preparation of straw biological charcoal：To originate in the maize straw of the Northwest as raw material, using retort in temperature
For 400 DEG C, cornstalk biological charcoal is prepared in stove under conditions of oxygen percent by volume 5%, organic carbon in prepared charcoal
Mass percentage content be 50%, the mass percentage content of ash content is 20%；
Step 2: charcoal field soil is cultivated：By the charcoal prepared by step 1 with 200g/m2Dosage October throw
Enter in hillside orchard soil, earthing thickness 10cm, decomposed six months under nature；
Step 3: rhizobium leguminosarum and gel-shaped series bacillus strain multiple cropping：Pea root is taken respectively with model 5ul oese
Knurl bacterium and the oese of gel-shaped series bacillus one enter to be configured to strain suspension in 10ml distilled water, take rhizobium leguminosarum strain
Suspension 50ul is inoculated in root nodule bacterium culture medium with rubbing method, is placed in constant incubator, is cultivated 3 days for 30 DEG C in temperature；Take glue
Freeze sample series bacillus strain suspension 50ul streak inoculations in silicate bacteria selective medium, be placed in constant incubator
In, 30 DEG C of temperature is cultivated 5 days；
Step 4: the expansion culture of strain：The oese of rhizobium leguminosarum single bacterium colony one brought back to life after cultivating is taken to add with 5ul oeses
Enter 10ml distilled water and be configured to strain suspension, take rhizobium leguminosarum strain suspension 80ul to be inoculated in the training of liquid root nodule bacteria liquid
Base 100ml is supported, is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 3 days, cultivation temperature is 30 DEG C；Will be multiple
Gel-shaped series bacillus single bacterium colony after culture living takes an oese to add 10ml distilled water and is configured to strain with 5ul oeses
Suspension, gel-shaped series bacillus strain suspension 80ul is taken to be inoculated in silicate bacteria selected liq culture medium 100ml,
It is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 5 days, cultivation temperature is 30 DEG C；
Step 5: the preparation of composite bacteria agent：The thalline cultivated using rotational tubby centrifuge collection step four is weighed, with pea root
Knurl bacterium strain quality, which accounts for 50%, gel-shaped series bacillus strain quality and accounts for 44%, honey 4%, wheat bran 2%, is prepared into mixing thalline,
Emulsified with homogenizer, freeze dryer be put into after emulsification, under vacuum conditions, -40 DEG C of snap frozens, block thalline is taken out after lyophilized,
Powder is broken into food masher, with serial dilutions spread plate, calculates viable count；Using the charcoal prepared by step 1 as
Adhesive, it is 1 by the thalli powder smashed and charcoal mass ratio:10 ratio prepares graininess microbial inoculum；
Step 6: the screening of leguminous green manure plant：Composite bacteria agent prepared by step 5 is inoculated in common vetch, big pigeon ash
Pea, white pea or French beans legume, efficient syntaxial system is built for strain, and select the optimal green manure pulse family of effect of inoculation
Plant；
Step 7: the nature of resigning from office and returning home of leguminous green manure plant is decomposed：By the composite bacteria agent prepared by step 5 with 200g/ before and after grain rains
m2The optimal green manure seeds of leguminous plant mixed seeding that is screened of dosage and step 5 fattened in step 2 straw biological charcoal
The field of six months, Routine Management produces 5 months, naturally decomposed by legume herb overburden soil table after harvesting, and orchard is made
The mass percentage content of composite biological organic fertilizer, wherein organic matter is 18.5%-25.7%, the mass percentage content of organic carbon
Mass percentage content for 22.3%-27.1%, N P and K is 27.4%-35.5% or 7.4%-10.3% or 8.6%-13.6%.
Preferably, the rhizobium culture medium prescription：Dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, sodium chloride 0.2g, sucrose
Or mannitol 10.0g, yeast extract 4.0g, mass percent concentration are 0.5% sodium molybdate solution 4.0ml, mass percent concentration
For 0.5% boric acid solution 4.0ml, calcium carbonate 5.0g, agar 18.0g, the mL mixtures of distilled water 1000, root nodule bacterium culture medium
PH value is 7.2-7.4.
Preferably, the silicate bacteria selective medium formula：Glucose 5.0g, magnesium sulfate 0.5g, calcium carbonate
0.1g, the g of disodium hydrogen phosphate 2.0, iron chloride, 0.005g, glass dust 1.0g, the g of agar 15.0, the mL of distilled water 1000 mixing
Thing, the pH value of silicate bacteria selective medium is 7.0.
The rhizobium leguminosarum（Rhizobium leguminosarum）With gel-shaped series bacillus
（Paenibacillusmucilaginosus）Purchase in China General Microbiological culture presevation administrative center, rhizobium leguminosarum
Deposit number is：Rl1.2327, gel-shaped series bacillus deposit number are 1.153.
Applicant has been explored on the basis of the positioning experiment of field so that cornstalk biological is carbon-based, rhizobium leguminosarum
（Rhizobium leguminosarum）With gel-shaped series bacillus（Paenibacillusmucilaginosus）With place
The orchard biological organic fertilizer supply model that the big pigeon hoary pea of legume kind is combined, and be produced as with technological service place
Purpose is in sky and water drought mountain cherry orchard, the positive popularization and application in apple orchard, acquirement gratifying results.
Applicant shows in experiments of the 2014-2016 in Tianshui Qinzhou District, Bei Daoqu, the ground experimental plot of Qinan County 3, with
Cornstalk biological charcoal cultivate soil is after six months, by rhizobium leguminosarum（Rhizobium leguminosarum）And gel-shaped
Series bacillus（Paenibacillusmucilaginosus）Composite bacteria agent is inoculated with big pigeon hoary pea and applies orchard management 3
Month, in the growth best fruiting period harvesting herb covering of big pigeon hoary pea, the orchard soil biological organic fertilizer producer of decomposed six months
Method, gathering the result after experiment soil samples and detecting is：
Influence of the biological organic fertilizer of table 1 to soil physico-chemical property
As can be seen from Table 1, test soil sample with normal soil sample compared with, test water content in soil sample, the soil weight, it is positive from
Sub- exchange capacity and pH have larger change compared with the control.
The influence that the biological organic fertilizer of table 2 forms to soil nutrient
From table 2 it can be seen that experiment soil sample, compared with common soil sample, experiment soil sample organic matter adds 35%-44% than control,
Soil alkali-hydrolyzable nitrogen increase 7%-8.3%, available phosphorus, effective potassium etc. has larger increase, hence it is evident that improves soil physical chemistry structure and battalion
Support content.
Influence of the biological organic fertilizer of table 3 to soil enzyme activities
As can be seen from Table 3, by Major Enzymes in soil urease, phosphatase, invertase Major Enzymes activity assays soil sample
Activity is improved.
The influence that the biological organic fertilizer of table 4 forms to soil Bacterial diversity
As shown in Table 4, shown by the detection to soil Bacterial diversity structure of community, organic fertilizer supply of the present invention can
The diversity of edaphon is significantly improved, makes the functional strain class in soil that significant change occur with quantity, from micro- life
Promoting the good formation of soil in terms of the composition of thing and vigor.
Influence of the biological organic fertilizer of table 5 to fruit output, sugar content
Apply and broadcast 1 year measurement fruit single plant yield of fertilizer of the present invention, soluble sugar and amino acid variable quantity, as shown in table 5.
From above-mentioned chart, organic fertilizer of the present invention can provide abundant nutriment and organic matter for soil,
Soil texture composition can be improved, coordinate soil, water, fertilizer, gas integral, cost is low, efficiency high, is a kind of the compound of safe and high quality
The production method of biomass organic fertilizer.The present invention makes full use of limited land resource, by agricultural wastes rational utilization,
Provided safeguard while environmental protection saves agricultural cost input for the production of green environmentally friendly high-quality Organic Fruit.
Claims (4)
1. a kind of preparation method of orchard composite biological organic fertilizer, the orchard composite biological organic fertilizer includes straw biological charcoal, beans
Section's green manure, rhizobium leguminosarum and gel-shaped series bacillus, it is characterised in that：It comprises the following steps：
Step 1: the preparation of straw biological charcoal：To originate in the maize straw of the Northwest as raw material, using retort in temperature
For 400 DEG C, cornstalk biological charcoal is prepared in stove under conditions of oxygen percent by volume 5%, organic carbon in prepared charcoal
Mass percentage content be 50%, the mass percentage content of ash content is 20%；
Step 2: charcoal field soil is cultivated：By the charcoal prepared by step 1 with 200g/m2Dosage October throw
Enter in hillside orchard soil, earthing thickness 10cm, decomposed six months under nature；
Step 3: rhizobium leguminosarum and gel-shaped series bacillus strain multiple cropping：Pea root is taken respectively with model 5ul oese
Knurl bacterium and the oese of gel-shaped series bacillus one enter to be configured to strain suspension in 10ml distilled water, take rhizobium leguminosarum strain
Suspension 50ul is inoculated in root nodule bacterium culture medium with rubbing method, is placed in constant incubator, is cultivated 3 days for 30 DEG C in temperature；Take glue
Freeze sample series bacillus strain suspension 50ul streak inoculations in silicate bacteria selective medium, be placed in constant incubator
In, 30 DEG C of temperature is cultivated 5 days；
Step 4: the expansion culture of strain：The oese of rhizobium leguminosarum single bacterium colony one brought back to life after cultivating is taken to add with 5ul oeses
Enter 10ml distilled water and be configured to strain suspension, take rhizobium leguminosarum strain suspension 80ul to be inoculated in the training of liquid root nodule bacteria liquid
Base 100ml is supported, is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 3 days, cultivation temperature is 30 DEG C；Will be multiple
Gel-shaped series bacillus single bacterium colony after culture living takes an oese to add 10ml distilled water and is configured to strain with 5ul oeses
Suspension, gel-shaped series bacillus strain suspension 80ul is taken to be inoculated in silicate bacteria selected liq culture medium 100ml,
It is placed in 250ml conical flasks, repeats to prepare 30 bottles, shaking table shaken cultivation 5 days, cultivation temperature is 30 DEG C；
Step 5: the preparation of composite bacteria agent：The thalline cultivated using rotational tubby centrifuge collection step four is weighed, with pea root
Knurl bacterium strain quality, which accounts for 50%, gel-shaped series bacillus strain quality and accounts for 44%, honey 4%, wheat bran 2%, is prepared into mixing thalline,
Emulsified with homogenizer, freeze dryer be put into after emulsification, under vacuum conditions, -40 DEG C of snap frozens, block thalline is taken out after lyophilized,
Powder is broken into food masher, with serial dilutions spread plate, calculates viable count；Using the charcoal prepared by step 1 as
Adhesive, it is 1 by the thalli powder smashed and charcoal mass ratio:10 ratio prepares graininess microbial inoculum；
Step 6: the screening of leguminous green manure plant：Composite bacteria agent prepared by step 5 is inoculated in common vetch, big pigeon ash
Pea, white pea or French beans legume, efficient syntaxial system is built for strain, and select the optimal green manure pulse family of effect of inoculation
Plant；
Step 7: the nature of resigning from office and returning home of leguminous green manure plant is decomposed：By the composite bacteria agent prepared by step 5 with 200g/ before and after grain rains
m2The optimal green manure seeds of leguminous plant mixed seeding that is screened of dosage and step 5 fattened in step 2 straw biological charcoal
The field of six months, Routine Management produces 5 months, naturally decomposed by legume herb overburden soil table after harvesting, and orchard is made
The mass percentage content of composite biological organic fertilizer, wherein organic matter is 18.5-25.7%, the mass percentage content of organic carbon
Mass percentage content for 22.3-27.1%, N P and K is 27.4-35.5% or 7.4-10.3% or 8.6-13.6%.
A kind of 2. preparation method of orchard composite biological organic fertilizer according to claim 1, it is characterised in that：The root nodule
Bacterium culture medium formula：Dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, sodium chloride 0.2g, sucrose or mannitol 10.0g, yeast extract
The boric acid solution that 4.0g, mass percent concentration are 0.5% sodium molybdate solution 4.0ml, mass percent concentration is 0.5%
4.0ml, calcium carbonate 5.0g, agar 18.0g, the mL mixtures of distilled water 1000, the pH value of root nodule bacterium culture medium is 7.2-7.4.
A kind of 3. preparation method of orchard composite biological organic fertilizer according to claim 1 or 2, it is characterised in that：It is described
Silicate bacteria selective medium formula：Glucose 5.0g, magnesium sulfate 0.5g, calcium carbonate 0.1g, the g of disodium hydrogen phosphate 2.0,
Iron chloride, 0.005g, glass dust 1.0g, the g of agar 15.0, the mL mixtures of distilled water 1000, silicate bacteria are selectively cultivated
The pH value of base is 7.0.
A kind of 4. preparation method of orchard composite biological organic fertilizer according to claim 1, it is characterised in that：The pea
Rhizobium and gel-shaped series bacillus are purchased in China General Microbiological culture presevation administrative center, rhizobium leguminosarum preservation
Numbering is：Rl1.2327, gel-shaped series bacillus deposit number are 1.153.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109121629A (en) * | 2018-08-31 | 2019-01-04 | 山西省农业科学院农业环境与资源研究所 | Northern orchard ecology conserves improvement of soil fertility method |
| CN111218286A (en) * | 2020-01-17 | 2020-06-02 | 上海理工大学 | Pigeon manure-based biochar material and preparation method and application thereof |
| CN114342748A (en) * | 2022-01-04 | 2022-04-15 | 中国科学院成都生物研究所 | Soil covering and three-dimensional fertilizer preservation method for hillside orchard |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109121629A (en) * | 2018-08-31 | 2019-01-04 | 山西省农业科学院农业环境与资源研究所 | Northern orchard ecology conserves improvement of soil fertility method |
| CN109121629B (en) * | 2018-08-31 | 2021-01-01 | 山西省农业科学院农业环境与资源研究所 | Ecological conserving soil fertility improvement method for northern orchard |
| CN111218286A (en) * | 2020-01-17 | 2020-06-02 | 上海理工大学 | Pigeon manure-based biochar material and preparation method and application thereof |
| CN114342748A (en) * | 2022-01-04 | 2022-04-15 | 中国科学院成都生物研究所 | Soil covering and three-dimensional fertilizer preservation method for hillside orchard |
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Application publication date: 20180406 |