CN101438642A - Method for repairing degenerated soil using lucerne and beneficial rhizosphere microorganisms - Google Patents
Method for repairing degenerated soil using lucerne and beneficial rhizosphere microorganisms Download PDFInfo
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- CN101438642A CN101438642A CNA2008102372431A CN200810237243A CN101438642A CN 101438642 A CN101438642 A CN 101438642A CN A2008102372431 A CNA2008102372431 A CN A2008102372431A CN 200810237243 A CN200810237243 A CN 200810237243A CN 101438642 A CN101438642 A CN 101438642A
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Abstract
The invention relates to a method for remedying degenerated soil resulting from water and soil loss, and the method is characterized by remedying the barren and compact soil by biological nitrogen fixation of rhizobia of purple alfalfa rhizosphere and microecological function of the rhizosphere of other beneficial microbes. The method comprises the following steps: dressing purple alfalfa seeds with acid-resistant alfalfa rhizobia 91522 and sowing the purple alfalfa seeds on the degenerated and untilled soil; only applying living bacteria or sterile bacteria suspension and water during a growing process of the purple alfalfa, wherein, the bacteria suspension comprises a bacillus megatherium suspension, an associative nitrogen-fixation bacteria suspension and a bacillus mucilaginosus suspension; harvesting the alfalfa plants after planting the purple alfalfa three months later, turning the alfalfa roots into in-situ soil, applying living cellulose-decomposing bacteria or sterile cellulose-decomposing bacteria suspension, and aging the in-situ soil for two months; tilling the soil to plant the alfalfa of the next season until the soil is remedied. The method can effectively improve microbial activities of the degenerated soil, increase all-purpose and effective nutrients in the degenerated soil and scarify the soil, and conserve water and soil and get a great quantity of fine pasture to develop the animal husbandry.
Description
Technical field
The present invention relates to the method for the soil that a kind of reparation degenerates because of water and soil loss.
Background technology
China's status of soil erosion is quite serious.For what repair that the lean soil degenerate because of water and soil loss at first needs is exactly to take measures to reduce or prevent erosion to continue to take place, and secondly suits the remedy to the case at lean soil with exactlying, apply crop need nutrition.At present, for reducing and the normal two kinds of means that adopt that prevent erosion, one is to use chemical amendment PAM (Polyacrylamide, polyacrylamide).PAM can prevent to show soil preferably and run off, if annual 90% of 3,300,000,000 tons of the ground surface soil numbers of dropouts of ploughing are washed away, means about 29.7 hundred million tons of (66t/hm
2) table soil be maintained and exempt from erosion.Yet because PAM is an anti-microbial effect, mainly by the physical action degraded, its residual monomer acrylamide is a kind of known carcinogenic substance, to the neural toxic effect of human body, can't large tracts of land promote.Another kind is used to reduce and the measure that prevents erosion is exactly a biological control measure.Biological control measure is with its minimum contamination, effect persistence and the preferred option of current repairing degenerated soil of becoming.Yet what the scholar often studied in biological control measure at present is respectively phytoremediation technology and microorganism recovery technique.And, more do not form any relevant patent for the also rare research of the complex effect of both combinations.
Summary of the invention
The objective of the invention is to deficiency at the prior art existence, a kind of method of utilizing alfalfa and beneficial rhizosphere microorganisms repairing degenerated soil is provided, utilizing the little Ecology Action of rhizosphere of the biological nitrogen fixation of alfalfa rhizosphere rhizobium and other beneficial rhizosphere microorganisms to repair barren compacted soil, is a kind of biological modification method of degenerated soil.
The beneficial rhizosphere microorganisms that the present invention selects for use has:
The beneficial rhizosphere microorganisms of selecting for use has:
Acidproof alfalfa Phylloxera bacteria strain 91522 (dragon is beautiful for Rhizobium meliloti 91522, Zhang Qin, Zhang Lei etc., and different pH values are inoculated the influence of rhizobium to the alfalfa yield and quality, grass cultivation journal, 2006,15 (5): 59-62) down
Bacillus megaterium (Bacillus megaterium, Chinese bacterium protects center bacterial strain ACCC10008)
Combination azotobacter (Associative-nitrogen-fixing Bacterium, list of references: Li Chunming, Zhang Lei etc., the rhizosphere combination azotobacter is to the yield increasing effect of corn wheat and Ipomoea batatas, Agricultural University Of Southwest's journal, 2003,25 (6): 506-509)
Potassium bacterium (separate at Southwestern University microbe research center for Bacillus mucilaginosus, colloid bacillus cereus)
Cellulose decomposition bacteria bacterial strain 1306 (Cellulose-Degradation Fungi 1306, list of references: Zhang Chao, Zhang Lei etc., Congo red transfer mechanism research in the fungi cellulase-producing medium, microorganism circular, 2006,33 (6): 12-16)
The method step of repairing is as follows:
(1) cultivates each bacterial classification bacteria suspension, be about 10 until each bacteria suspension viable bacteria bulk concentration
6Individual/ml, alfalfa seed is cultivated in the laboratory.
(2) use acidproof alfalfa Phylloxera bacteria strain 91522 to the clover seed dressing, at soil upper seeding wheel to be repaired.
(3) rationally water, apply bacillus megaterium, combination azotobacter, potassium bacterium bacteria suspension at ordinary times.
(4) treat that alfalfa enters the reproductive growth after date, the results alfalfa plants is used for herding.Radix Medicaginis is ploughed under in the soil, apply the bacteria suspension of cellulose decomposition bacteria bacterial strain 1306, original position soil was macerated ripe two months.
(5) turn over and carry out the plantation of next batch clover.
The plantation of the alfalfa of several batches of compounding application beneficial rhizosphere bacterium of process, degenerated soil just can recover soil fertility substantially.
The present invention utilizes the leguminous plant and the useful rhizospheric microorganism of symbiotic nitrogen fixation, builds the plant rhizosphere micro-ecological environment that gathers organic matter, Soil Nitrogen, phosphorus, potassium and edaphon rapidly.Be soil nitrogen pick-up for example by symbiotic nitrogen fixation; Microbial decomposition by plant rhizosphere secretion, plant residue increases the soil organic matter; Utilize functional beneficial rhizosphere microorganisms such as phosphobacteria to decompose to close in the soil mineral and hold attitude for available phosphorus etc.By plant one to two season legume, and join and execute microorganism formulation, make soil fertility obtain repairing.This method not only can effectively improve the microbial activities amount of degenerated soil, increases full effect of soil and available nutrient, loose soil property, and can also conserve water and soil, and obtain a large amount of high quality forages to develop animal husbandry.
Embodiment
Below relend and help embodiment to be described in more detail the present invention; but should be appreciated that following examples only are illustrative; it is not limiting the scope of the invention; the conversion of any those of ordinary skill in the art under the spiritual essence that does not break away from claim protection of the present invention all should be within protection scope of the present invention.
Embodiment 1: for examination soil be purple matrix soil, does not use rhizobium to dress seed, and does not also use any bacterial classification, and planting pot alfalfa three was ploughed under in the original position soil behind each and every one moon, did not add cellulose decomposition bacteria, macerated ripe two months.Every index increasing rate of soil such as table 1.
Table 1
Embodiment 2: for examination soil is purple matrix soil, use acidproof rhizobium melioti 91522 seed dressings, use bacillus megaterium, azotobacter, the potassium bacterium bacterium of going out, promptly lose bioactive each 2.5ml/kg soil of dead thalline bacteria suspension, the plantation alfalfa is after three months, plough under in the original position soil, add the went out bacteria suspension 2.5ml/kg soil of bacterium of cellulose decomposition bacteria, macerated ripe two months.Every index increasing rate of soil such as table 2.
Table 2
Embodiment 3: for examination soil is purple matrix soil, use acidproof rhizobium melioti 91522 seed dressings, use the went out bacteria suspension 2.5ml/kg soil of bacterium of bacillus megaterium live body bacteria suspension 2.5ml/kg soil, azotobacter live body bacteria suspension 5ml/kg soil, potassium bacterium, the plantation alfalfa is after three months, plough under in the original position soil, add cellulose decomposition bacteria live body bacteria suspension 2.5ml/kg soil, macerated ripe two months.Every index increasing rate of soil such as table 3.
Table 3
Embodiment 4: for examination soil is purple matrix soil, use acidproof rhizobium melioti 91522 seed dressings, use bacillus megaterium went out bacteria suspension 2.5ml/kg soil, azotobacter live body bacteria suspension 5ml/kg soil, the potassium bacterium live body bacteria suspension 5ml/kg soil of bacterium, the plantation alfalfa is after three months, plough under in the original position soil, add cellulose decomposition bacteria live body bacteria suspension 5ml/kg soil, macerated ripe two months.Every index increasing rate of soil such as table 4.
Table 4
Embodiment 5: for examination soil is purple matrix soil, use acidproof rhizobium melioti 91522 seed dressings, use bacillus megaterium live body bacteria suspension 5ml/kg soil, azotobacter live body bacteria suspension 5ml/kg soil, potassium bacterium live body bacteria suspension 2.5ml/kg soil, the plantation alfalfa is after three months, plough under in the original position soil, add the went out bacteria suspension 2.5ml/kg soil of bacterium of cellulose decomposition bacteria, macerated ripe two months.Every index increasing rate of soil such as table 5.
Table 5
Embodiment 6: for examination soil is purple matrix soil, use acidproof rhizobium melioti 91522 seed dressings, use bacillus megaterium live body bacteria suspension 2.5ml/kg soil, azotobacter live body bacteria suspension 2.5ml/kg soil, potassium bacterium live body bacteria suspension 5ml/kg soil, the plantation alfalfa is after three months, plough under in the original position soil, add the went out bacteria suspension 2.5ml/kg soil of bacterium of cellulose decomposition bacteria, macerated ripe two months.Every index increasing rate of soil such as table 6.
Table 6
With example 1, promptly blank is compared, and total soil nitrogen totally is increase trend, and the proportioning with example 2 is the most obvious to the increase of total soil nitrogen especially.And what use in the example 2 all is to lose bioactive bacteria suspension, and this illustrates that except the required full nitrogen of plant growing the microorganism live body also has certain consumption to total soil nitrogen.Example 3 is handled the content of organic matter than other significantly to be increased, illustrate in soil organic matter cumulative process, and this kind proportioning, promptly the cellulose decomposition bacteria of 2.5ml/kg soil bacillus megaterium, 5ml/kg soil azotobacter and 2.5ml/kg soil has facilitation to it.The facilitation that 4 pairs of soil alkali-hydrolyzable nitrogens of example improve is obvious, illustrates that except that bacillus megaterium the rational proportion of combination azotobacter, potassium bacterium and cellulose decomposition bacteria can improve the quick-acting nitrogen contents of soil.The existing research conclusion of this conclusion and this seminar is coincide, and promptly cellulose decomposition bacteria can be supplied with the nitrogen fixation energy, promotes the accumulation of environment nitrogen.This handles than other and handles to the soil available phosphorus increase obviously, even bacillus megaterium is described the effect of decomposing soil mineral phosphorus is arranged, hungry soil has only whole microflora developed, there is the rely organic matter of breeding of enough microorganisms to supply with, even without phosphate solubilizing bacteria, soil available phosphorus still can comparatively fast improve.Example 5 and example 6 microbial biomasss significantly improve the raising that explanation bacillus megaterium and cellulose decomposition bacteria can promote the soil organic matter, and what illustrate simultaneously that this research institute adopts has suitable using value aspect fostering and apply fertilizer by the autonomous cellulose decomposition bacteria that separates of seminar improving the soil.
Claims (5)
1. utilize alfalfa and beneficial rhizosphere microorganisms repairing degenerated soil method, it is characterized in that on the out of crop soil of degenerating, use acidproof rhizobium melioti 91522 to the alfalfa seed dressing, the sowing alfalfa seed; Only use bacteria suspension and the water of the live body or the bacterium of going out in the alfalfa process of growth, bacteria suspension comprises bacillus megaterium, combination azotobacter, potassium bacterium bacteria suspension; After three months, the results alfalfa plants ploughs under Radix Medicaginis in the original position soil, applies the bacteria suspension of the cellulose decomposition bacteria live body or the bacterium of going out in the plantation alfalfa, and original position soil was macerated ripe two months; Turn over and carry out the plantation of next batch clover, repaired until soil.
2. method according to claim 1 is characterized in that, the bacillus megaterium live body of described adding or the bacteria suspension of the bacterium of going out are 2.5-5ml/kg soil.
3. method according to claim 1 is characterized in that, the azotobacter live body of described adding or the bacteria suspension of the bacterium of going out are 2.5-5ml/kg soil.
4. method according to claim 1 is characterized in that, the potassium bacterium live body of adding or the bacteria suspension of the bacterium of going out are 2.5-5ml/kg soil.
5. method according to claim 1 is characterized in that, the cellulose decomposition bacteria live body of described adding or the bacteria suspension of the bacterium of going out are 2.5-5ml/kg soil.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990799B (en) * | 2009-08-31 | 2013-05-22 | 罗林松 | Biological nitrogen production method |
| CN106489344A (en) * | 2016-10-20 | 2017-03-15 | 黑龙江省科学院大庆分院 | A kind of method that rhizobium melioti improves soil quality |
| CN106879361A (en) * | 2017-04-27 | 2017-06-23 | 中国农业大学 | A kind of method of Degraded Leymus chinense Grassland after-culture clover |
| CN107879842A (en) * | 2017-11-16 | 2018-04-06 | 天水师范学院 | A kind of preparation method of orchard composite biological organic fertilizer |
| CN108633369A (en) * | 2018-04-23 | 2018-10-12 | 中国林业科学研究院森林生态环境与保护研究所 | A method of repairing South-West Asia alpine region degenerated soil using nitrogen fixing tree Anlnus nepalensis |
| CN113348982A (en) * | 2021-06-24 | 2021-09-07 | 中国科学院东北地理与农业生态研究所 | Method for constructing mixed-sowing grassland by additionally sowing alfalfa in slightly degraded leymus chinensis meadow |
| CN113940165A (en) * | 2021-09-23 | 2022-01-18 | 西安理工大学 | Water and soil root soil-fixing method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5525139A (en) * | 1993-07-12 | 1996-06-11 | Gill; Paul E. | Process for bioremediation of soils |
| CN100400188C (en) * | 2005-03-16 | 2008-07-09 | 中国科学院南京土壤研究所 | Biological double inoculating method for reducing polychlorinated biphenyls and polycyclic aromatic hydrocarbons in polluted soil |
| CN101229550A (en) * | 2007-01-24 | 2008-07-30 | 中国科学院生态环境研究中心 | Method of increasing gesarex polluted soil phyto remediation efficiency |
| CN101306427A (en) * | 2007-05-17 | 2008-11-19 | 中国科学院生态环境研究中心 | Method for increasing organic pollutant degradation efficiency in soil |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990799B (en) * | 2009-08-31 | 2013-05-22 | 罗林松 | Biological nitrogen production method |
| CN106489344A (en) * | 2016-10-20 | 2017-03-15 | 黑龙江省科学院大庆分院 | A kind of method that rhizobium melioti improves soil quality |
| CN106879361A (en) * | 2017-04-27 | 2017-06-23 | 中国农业大学 | A kind of method of Degraded Leymus chinense Grassland after-culture clover |
| CN106879361B (en) * | 2017-04-27 | 2020-04-14 | 中国农业大学 | Method for reseeding alfalfa on deteriorated leymus chinensis grassland |
| CN107879842A (en) * | 2017-11-16 | 2018-04-06 | 天水师范学院 | A kind of preparation method of orchard composite biological organic fertilizer |
| CN108633369A (en) * | 2018-04-23 | 2018-10-12 | 中国林业科学研究院森林生态环境与保护研究所 | A method of repairing South-West Asia alpine region degenerated soil using nitrogen fixing tree Anlnus nepalensis |
| CN108633369B (en) * | 2018-04-23 | 2020-06-16 | 中国林业科学研究院森林生态环境与保护研究所 | Method for repairing degraded soil in southwest Asia alpine region by utilizing nitrogen-fixing tree species drynaria |
| CN113348982A (en) * | 2021-06-24 | 2021-09-07 | 中国科学院东北地理与农业生态研究所 | Method for constructing mixed-sowing grassland by additionally sowing alfalfa in slightly degraded leymus chinensis meadow |
| CN113940165A (en) * | 2021-09-23 | 2022-01-18 | 西安理工大学 | Water and soil root soil-fixing method |
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