CN107837227A - A kind of blackhead skin care item and preparation method thereof of dispelling of the cell extract of trunk containing Chinese violet - Google Patents
A kind of blackhead skin care item and preparation method thereof of dispelling of the cell extract of trunk containing Chinese violet Download PDFInfo
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- CN107837227A CN107837227A CN201711272571.0A CN201711272571A CN107837227A CN 107837227 A CN107837227 A CN 107837227A CN 201711272571 A CN201711272571 A CN 201711272571A CN 107837227 A CN107837227 A CN 107837227A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention discloses a kind of blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet, it contains the raw material of following weight/mass percentage composition：Chinese violet trunk cell extract 0.1~6%, grape seed extract 1~10%, gadol extract 0.5~5% and mangosteen extract 1~7%.Meanwhile the invention also discloses the preparation method of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet.Blackhead skin care item provided by the invention of dispelling contain Chinese violet trunk cell extract and a variety of natural plant active skin care ingredients, its penetrating power is extremely strong, sebum, cell bits and the tough stains in pore can effectively be softened, conditioning skin fat secretion simultaneously, there is the effect of dispelling blackhead, pore refining and preventing blackhead from recurring.
Description
Technical field
The invention belongs to cosmetic technical field, and in particular to a kind of blackhead skin care of dispelling of the cell extract of trunk containing Chinese violet
Product and preparation method thereof.
Background technology
Blackhead is also known as blackhead, one kind " bolt " the sample thing being mainly made up of sebum, cell bits and bacterium, is blocked in hair
Capsule opening and formed, plus the dust in air, dirt and oxidation, make a gradual blackening of its ingress of air,
So-called blackhead is formed, it is characterized in that the stain in the pore substantially expanded, is blacked after extrusion shaped like small worm, top.It is black
Head is hardened fat obturator, typically occurs in the positions such as forehead, the nose of face, when grease gland swashs by intermuscular needling, pore
When obstruction is caused full of unnecessary grease, in nose and its peripheral part, greasy sensation is often had.These greases are final
It can harden, turn into the dot of black after oxidized, these dots are exactly the grease obturator referred to as blackhead.Blackhead is
Personal skin quality and outside environmental elements with reference to and caused by, any age, the people of any skin quality all occur the possibility of blackhead.
It is main by the following method that blackhead is dispelled at present：(1) will be ground using abrasive particle exposed to the blackhead of skin surface
Remove, this method effect is poor, is unable to reach the purpose of dredging pore；(2) blackhead is pulled out from pore, such as various noses
Patch, this method are pullled skin, and the relaxation of pore mouth can be caused to become big, restrained not in time after, blackhead can faster occur；
(3) by smear blackhead product of dispelling add massage or it is stifling allow blackhead emulsification to soften easily to discharge from pore, this method uses
Cumbersome, complex steps, the time is longer, and it is dry to be finished skin meeting.There is the product of some blackheads of dispelling on the market, can reach
Certain blackhead effect of dispelling, but some chemical compositions can be added more in these products, long-term use can damage skin, cause grease
The various problems such as dyssecretosis, make blackhead easily recur.
The content of the invention
Based on this, a kind of trunk containing Chinese violet is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The blackhead skin care item of dispelling of cell extract, it contains Chinese violet trunk cell extract and a variety of natural plant active skin care ingredients,
Penetrating power is extremely strong, can effectively soften sebum, cell bits and tough stains in pore.
To achieve the above object, the technical scheme taken of the present invention is：A kind of dispelling for cell extract of trunk containing Chinese violet is black
Head skin care item, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 0.1~6%, grape seed extract 1
~10%, gadol extract 0.5~5% and mangosteen extract 1~7%.
Preferably, the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet contain the original of following weight/mass percentage composition
Material：Chinese violet trunk cell extract 0.5%, grape seed extract 6.5%, gadol extract 3.5% and mangosteen extract
3%.
Preferably, the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet contain the original of following weight/mass percentage composition
Material：Chinese violet trunk cell extract 2%, grape seed extract 1.5%, gadol extract 2% and mangosteen extract 1.5%.
Preferably, the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet contain the original of following weight/mass percentage composition
Material：Chinese violet trunk cell extract 4%, grape seed extract 3%, gadol extract 1% and mangosteen extract 5.5%.
Preferably, the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet contain the original of following weight/mass percentage composition
Material：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, gadol extract 1.5% and mangosteen extract 4%.
Present inventor has found by substantial amounts of research, by Chinese violet trunk cell extract, grape seed extract, red scape
Its extract and mangosteen extract prepare skin care item, and synergy well is produced between each raw material, can effectively dispel blackhead, receive
Contracting pore, while adjust skin oil and fat secretion.The compatibility amount of raw material is that inventor gets by the analysis of substantial amounts of Research statistics,
Each raw material can play good synergy in the amount ranges, not have any side-effects simultaneously.
It is another object of the present invention to provide the preparation of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet
Method.
To achieve the above object, the technical scheme taken of the present invention is：A kind of dispelling for cell extract of trunk containing Chinese violet is black
The preparation method of head skin care item, comprises the following steps：
(1) described Chinese violet trunk cell extract, grape seed extract, gadol extract and mangosteen are weighed in proportion
Extract, mix；
(2) mixture in step (1) is slowly added to the skin care made of other acceptable auxiliary materials of skin care field
In product matrix, stir, produce the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet.
Preferably, the skin care item include facial mask, face cream, emulsion, toner, mildy wash, face cleaning cream and mildy wash.
Preferably, the preparation method of the Chinese violet trunk cell extract comprises the following steps：(1) it is newborn to collect Chinese violet tree
Branch, cleaning, is cut into 6cm, soaks 8min in 80mg/L L-AA；Used after being put into 75% alcohol solution dipping 8min
Aseptic water washing；It is then placed in 30% aqueous hydrogen peroxide solution and soaks 20min, then with aseptic water washing 15min.(2) take
Lower Chinese violet tree body material contains cambial position, is put into NAA containing 1mg/L, 3mg/L BA and 1.5mg/L caseinhydrolysates
Light culture on MS solid mediums, cultivation temperature are 28 DEG C, and culture humidity is 75%, incubation time 20d.(3) squamous subculture：
Cambial cell is inoculated in the MS liquid of 2,4-D containing 1.5mg/L, 2mg/L BA, 2mg/L caseinhydrolysates and 2mg/L activated carbons
Shaking table light culture in body culture medium, cultivation temperature are 30 DEG C, cultivate rotating speed 120r/min, incubation time 20d, MS Liquid Culture
Bead can be added in base.(4) by the cell line of squamous subculture with 1 × 104/ mL inoculum density moves into gas-lifting type biological respinse
In device expand culture, during culture temperature with rotating speed with being consistent during squamous subculture, incubation time 10d.(5) collect and expand
Obtained Chinese violet trunk cell is cultivated, in -30 DEG C of pre-freezes 3 hours after quick-frozen 2 hours in liquid nitrogen；Then thaw 30 minutes；Instead
Multiple 2 times after 25 DEG C of placement 10min.Then 60 mesh sieves can be crossed by being crushed to, and the mass fraction for adding 5 times of volumes of dry cell wt is
75% ethanol, in 30 DEG C of stirring extraction 10h, it is then placed in ultrasonic separation extractor and carries out strong Ultrasonic Pulverization cytoplasm, surpass
Sound extract solution filters, and collects filtrate, concentrates, is drying to obtain.
The MS culture mediums are a kind of conventional plant cultures, available for evoked callus, it can also be used to embryo, stem
The culture of section, stem apex and flower pesticide；NAA is the abbreviation of methyl α-naphthyl acetate, and methyl α-naphthyl acetate is a kind of common auximone, can promote cell
Division and expansion；BA is the abbreviation of benzyl aminoadenine, and benzyl aminoadenine is a kind of basic element of cell division, and wherein 6-BA compares
It is common；Caseinhydrolysate has obvious facilitation to the propagation of cell.
Present invention also offers a kind of blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet, contain containing following quality percentage
The raw material of amount：Chinese violet trunk cell extract 0.1~6%, grape seed extract 1~10%, gadol extract 0.5~5%,
Mangosteen extract 1~7%, pearl powder 10~20%, asparagus juice 10~20% and honey 5~10%.
Preferably, the blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet, the original containing following weight/mass percentage composition
Material：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, gadol extract 1.5%, mangosteen extract 4%, treasure
Pearl powder 15%, asparagus juice 15% and honey 8%.
Present invention also offers the preparation method of the blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet, including it is following
Step：
(1) described Chinese violet trunk cell extract, grape seed extract, gadol extract and mangosteen are weighed in proportion
Extract, mix standby；
(2) pearl powder, asparagus juice, honey and appropriate water are tuned into paste, the mixture added in step (1), slowly
Stir, produce the blackhead facial mask of dispelling.
Relative to prior art, beneficial effects of the present invention are：Blackhead skin care item provided by the invention of dispelling contain Chinese violet tree
Stem cell extract and a variety of natural plant active skin care ingredients, its penetrating power is extremely strong, can effectively soften sebum in pore,
Cell is considered to be worth doing and tough stains, while conditioning skin fat secretion, has and dispels blackhead, pore refining and the work(for preventing blackhead from recurring
Effect.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.It should be noted that the percentage of raw material is all mass percent in the present invention.
Embodiment 1
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 0.1%, grape seed extract 1%, rhodiola root
Extract 0.5%, mangosteen extract 1%, pearl powder 15%, asparagus juice 15% and honey 8%, surplus are water.
The preparation method of the skin care item comprises the following steps：(1) described Chinese violet trunk cell extraction is weighed in proportion
Thing, grape seed extract, gadol extract and mangosteen extract, mix standby；(2) by pearl powder, asparagus juice, honey and suitable
The water of amount is tuned into paste, the mixture added in step (1), is slowly stirred uniformly, produces the skin care item.
The preparation method of the Chinese violet trunk cell extract comprises the following steps：(1) the newborn branch of Chinese violet tree is collected, clearly
Wash, be cut into 6cm, soak 8min in 80mg/L L-AA；Sterilized water is used after being put into 75% alcohol solution dipping 8min
Rinse；It is then placed in 30% aqueous hydrogen peroxide solution and soaks 20min, then with aseptic water washing 15min.(2) Chinese violet is removed
Tree body material contains cambial position, is put into NAA containing 1mg/L, the MS solids of 3mg/L BA and 1.5mg/L caseinhydrolysates
Light culture on culture medium, cultivation temperature are 28 DEG C, and culture humidity is 75%, incubation time 20d.(3) squamous subculture：It will be formed
Confluent monolayer cells are inoculated in the MS Liquid Cultures of 2,4-D containing 1.5mg/L, 2mg/L BA, 2mg/L caseinhydrolysates and 2mg/L activated carbons
Shaking table light culture in base, cultivation temperature are 30 DEG C, cultivate rotating speed 120r/min, can in incubation time 20d, MS fluid nutrient medium
Add bead.(4) by the cell line of squamous subculture with 1 × 104/ mL inoculum density, which is moved into airlift bioreactor, to be expanded
Big culture, during culture temperature with rotating speed with being consistent during squamous subculture, incubation time 10d.(5) expansion is collected to cultivate
The Chinese violet trunk cell arrived, in -30 DEG C of pre-freezes 3 hours after quick-frozen 2 hours in liquid nitrogen；Then thaw 30 minutes；Repeatedly after 2 times
10min is placed in 25 DEG C.Then 60 mesh sieves can be crossed by being crushed to, and the mass fraction for adding 5 times of volumes of dry cell wt is 75% second
Alcohol, in 30 DEG C of stirring extraction 10h, it is then placed in ultrasonic separation extractor and carries out strong Ultrasonic Pulverization cytoplasm, ultrasonic extraction
Liquid filters, and collects filtrate, concentrates, is drying to obtain.
The grape seed extract is grape pip alcohol extracting thing, is prepared by following methods：By grape pip flour, add
The mass fraction of its 8 times of volume of weight is 75% ethanol, 80 DEG C of refluxing extractions 2 times, each 1h, merges extract solution, is filtered, filter
Ethanol is removed in liquid decompression, is drying to obtain.
The gadol extract is rhodiola root alcohol extracting thing, is prepared by following methods：By rhodiola root flour, add
The mass fraction of its 10 times of volume of weight is 70% ethanol, 85 DEG C of refluxing extractions 3 times, each 1h, merges extract solution, is filtered,
Filtrate decompression removes ethanol, is drying to obtain.
The mangosteen extract is mangosteen alcohol extracting thing, is prepared by following methods：Mangosteen is weighed, crushes, adds
Enter the mass fraction of 8 times of volumes of coarse powder weight for 85% ethanol solution, refluxing extraction 3 times under the conditions of 80 DEG C, each 1.5h；
Extract solution filters, and merging filtrate, is concentrated under reduced pressure, is drying to obtain.
Embodiment 2
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 6%, grape seed extract 10%, rhodiola root carry
Thing 5%, mangosteen extract 7%, pearl powder 15%, asparagus juice 15% and honey 8% are taken, surplus is water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 3
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 0.5%, grape seed extract 6.5%, red scape
Its extract 3.5%, mangosteen extract 3%, pearl powder 15%, asparagus juice 15% and honey 8%, surplus are water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 4
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 2%, grape seed extract 1.5%, rhodiola root
Extract 2%, mangosteen extract 1.5%, pearl powder 15%, asparagus juice 15% and honey 8%, surplus are water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 5
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 4%, grape seed extract 3%, rhodiola root carry
Thing 1%, mangosteen extract 5.5%, pearl powder 15%, asparagus juice 15% and honey 8% are taken, surplus is water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 6
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, pearl powder 15%, asparagus juice 15% and honey 8%, surplus are water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 7
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, pearl powder 10%, asparagus juice 10% and honey 5%, surplus are water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 8
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are face
Film, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, pearl powder 20%, asparagus juice 20% and honey 10%, surplus are water.
The preparation method of the skin care item and the preparation method of extract are the same as embodiment 1.
Embodiment 9
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are to wash one's face
Milk, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, Cocoamidopropyl betaine 5%, stearine 15%, hyaluronic acid 3%,
Surplus is water.
The preparation method of the skin care item comprises the following steps：(1) described Chinese violet trunk cell extraction is weighed in proportion
Thing, grape seed extract, gadol extract and mangosteen extract, mix standby；(2) by stearine, hyaluronic acid
Stirred with appropriate water, and be warming up to 80 DEG C, then add Cocoamidopropyl betaine, continue to stir, cooled
The mixture added after to 40 DEG C in step (1), it is slowly stirred uniformly, produces the skin care item.
The preparation method of the extract is the same as embodiment 1.
Embodiment 10
A kind of embodiment of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet of the invention, the skin care item are skin toner
Water, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, glycerine 5%, propane diols 5%, butanediol 5%, glycine betaine 2.5%, soluble protein
Polysaccharide 1%, carbomer 0.2%, hyaluronic acid 0.03%, essence 0.2% and preservative 0.5%, surplus are water.
The preparation method of the skin care item comprises the following steps：(1) described Chinese violet trunk cell extraction is weighed in proportion
Thing, grape seed extract, gadol extract and mangosteen extract, mix standby；(2) by glycerine, propane diols, butanediol, sweet tea
Dish alkali, soluble protein polysaccharide, carbomer, hyaluronic acid and appropriate water stir, and are warming up to 70 DEG C, then add fragrant
Essence and preservative, continue to stir, and are cooled to the mixture added after 37 DEG C in step (1), are slowly stirred uniformly, produce institute
State skin care item.
The preparation method of the extract is the same as embodiment 1.
Embodiment 11
The present embodiment is tested the using effect of skin care item by taking skin care item in embodiment 1~6 as an example.
(1) method of testing
1st, skin care item of the present invention are dispelled the method for testing of blackhead and pore refining effect：Choose 300 health volunteers, skin
There is obvious blackhead, the age is between 30~55 years old.All subjects are randomly divided into 6 test groups, respectively using embodiment 1
Skin care item in~6.The subject of all test groups after clear water face cleaning with taking appropriate skin care item to be uniformly applied to face, 15min
After be eluted with water, dry.Trial employment period prevents solar exposure, and prohibits the use of other skin care item.Use 4 times weekly, continuously make
After one month, dispel blackhead and detection and the record of pore refining effect are carried out to all subjects.
2nd, solute effect method of testing of the skin care item of the present invention to blackhead：20 health volunteers are chosen, are carried using acne
The blackhead of device extraction subject is taken, is positioned in 96 orifice plates, it is black using the skin care item submergence in about 100uL embodiments 1~6 respectively
Head, be used below gray card [John Grinder Macbeth company
ColorCheckerTM (GretagMacbeth ColorChecker) color renditions chart-neutrality 8 (0.23)
CC20], and measured using microscope in the case of without stirring under 40 times.96 orifice plate Parafilms are covered to avoid steaming
Hair, each test solution is removed when 24h and is dried overnight it.Scale of the amount of the blackhead of dissolving by expert's detection by 1-7
Classification.
(2) effect assessment standard
1st, the blackhead effect of dispelling of skin care item of the present invention is evaluated using standard as described in Table 1：
The blackhead effect assessment standard of dispelling of 1 skin care item of the present invention of table
2nd, the effect of the pore refining of skin care item of the present invention is evaluated using skin pore aperture minification, specific method is such as
Under：
Using skin image analyzer test skin pore aperture, nose areas is selected as test position.Selection is to same
One position pore shooting, analysis draw pore pore size.Before on-test, first to the hair of each subject's nose areas
Hole is detected, and is recorded；After skin care item of the present invention one month, the pore of subject's same position is examined again
Survey, and record.The pore aperture minification of skin is calculated using below equation：Pore aperture minification=(numerical value-examination before experiment
Test rear numerical value)/experiment before numerical value × 100%.Pore aperture minification is bigger, shows that pore refining effect is better.
3rd, solute effect of the skin care item of the present invention to blackhead, the amount of the blackhead of dissolving are evaluated using the amount of the blackhead of dissolving
It is classified by expert's detection by 1-7 scale, 1 is insoluble, and 7 be to be completely dissolved, specifically as shown in table 2:
The blackhead meltage grade scale of table 2
| Grade numbers | Explanation |
| 7 | It is completely dissolved |
| 6 | Most of dissolving, it can be seen that the remnants of initial blackhead |
| 5 | See the initial blackhead more than 25% |
| 4 | See the initial blackhead more than 50% |
| 3 | See the initial blackhead more than 75% |
| 2 | See the initial blackhead more than 90% |
| 1 | See the initial blackhead (be most similar to initial graph as) more than 95% |
4th, the evaluation criterion of security of the present invention is as shown in table 3：
The evaluation criterion of 3 security of the present invention of table
(3) test result
The using effect of all subjects is counted, while blackhead meltage experimental result is analyzed.As a result
As shown in table 4.
The skin care item measure of merit result of the present invention of table 4
As shown in Table 4, skin care item are respectively provided with the effect of extraordinary dispel blackhead and pore refining in the embodiment of the present invention 1~6
Fruit, the total effective rate of its blackhead of dispelling is up to 100% (embodiment 3,4,6)；It can effectively dissolve blackhead simultaneously.Subject, which is reflected, to be used
After one month, facial blackhead disappears skin care item of the present invention, and pore attenuates, and fat secretion amount is reduced, and skin becomes dry and comfortable, greasy feeling
Decline.It is efficiently gentle and all subjects do not occur phenomena such as allergy, general red, itch or long acne in use.Its
In, the skin care item using effect in embodiment 6 is best.To the skin care item using effect test result in embodiment 7~10 and this
Embodiment is similar, and detailed data is omitted.
Embodiment 12
The present invention dispels blackhead skin care item, the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 0.1~
6%th, grape seed extract 1~10%, gadol extract 0.5~5% and mangosteen extract 1~7%.For research present invention shield
Influence of the skin product raw material usage amount to effect, the present embodiment are studied the usage amount of each raw material of skin care item.
(1) experimental design
The present embodiment inquires into the use of Chinese violet trunk cell extract by taking the usage amount of Chinese violet trunk cell extract as an example
Measure the influence to skin care item using effect of the present invention.Contrived experiment group 1~3 and control group 1~2, it is specific as shown in table 5.Respectively
The usage amount of other raw materials of group is with embodiment 6, and the preparation method of each raw material extracting method and skin care item is the same as embodiment 1.
The experimental group of table 5 designs
(2) experimental method
The experimental method and effect assessment standard of the present embodiment with embodiment 11, wherein, blackhead effect assessment of dispelling experiment in
Every group of skin care item subject is 30 people.
(3) experimental result
The effect for the skin care item that each group is prepared is evaluated, as a result as shown in table 6：
The experimental result of table 6
From above-mentioned experimental result, the usage amount of skin care item Chinese violet trunk cell extract of the present invention is in 0.1~6% matter
Dispel well blackhead and pore refining effect can be obtained when (experimental group 1~3) between amount percentage composition, not in this range
Usage amount (control group 1~2) can influence the using effect of skin care item.The usage amount of each raw material of skin care item of the present invention is inventor
The best proportion counted by many experiments, the usage amount of each raw material can ensure in the range of weight/mass percentage composition of the present invention
Skin care item obtain good using effect, and any one raw material usage amount increases or decreases the collaboration effect that can all influence between raw material
Should, the effect of so as to influence skin care item.As control group 2 adds the usage amount of Chinese violet trunk cell extract, but it is used
Effect does not improve accordingly, has declined on the contrary.Grape seed extract, gadol extract and mangosteen extract usage amount
Experimental result is similar with the present embodiment, and specific data are omitted.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet, it is characterised in that contain following weight/mass percentage composition
Raw material：Chinese violet trunk cell extract 0.1~6%, grape seed extract 1~10%, the and of gadol extract 0.5~5%
Mangosteen extract 1~7%.
2. the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet according to claim 1, it is characterised in that containing with
The raw material of lower weight/mass percentage composition：Chinese violet trunk cell extract 0.5%, grape seed extract 6.5%, gadol extract
3.5% and mangosteen extract 3%.
3. the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet according to claim 1, it is characterised in that containing with
The raw material of lower weight/mass percentage composition：Chinese violet trunk cell extract 2%, grape seed extract 1.5%, gadol extract 2%
With mangosteen extract 1.5%.
4. the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet according to claim 1, it is characterised in that containing with
The raw material of lower weight/mass percentage composition：Chinese violet trunk cell extract 4%, grape seed extract 3%, the and of gadol extract 1%
Mangosteen extract 5.5%.
5. the blackhead skin care item of dispelling of a cell extract of trunk containing Chinese violet according to claim 1, it is characterised in that contain
The raw material of following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, gadol extract
1.5% and mangosteen extract 4%.
6. the preparation side of the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet according to any one of Claims 1 to 5
Method, it is characterised in that comprise the following steps：
(1) described Chinese violet trunk cell extract, grape seed extract, gadol extract and mangosteen extraction are weighed in proportion
Thing, mix；
(2) mixture in step (1) is slowly added to the skin care item base made of other acceptable auxiliary materials of skin care field
In matter, stir, produce the blackhead skin care item of dispelling of the cell extract of trunk containing Chinese violet.
7. preparation method according to claim 6, it is characterised in that skin care item include facial mask, face cream, emulsion, toner,
Mildy wash, face cleaning cream and mildy wash.
8. a kind of blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet, it is characterised in that contain following weight/mass percentage composition
Raw material：Chinese violet trunk cell extract 0.1~6%, grape seed extract 1~10%, gadol extract 0.5~5%, mangosteen
Extract 1~7%, pearl powder 10~20%, asparagus juice 10~20% and honey 5~10%.
9. the blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet according to claim 8, it is characterised in that its feature exists
In the raw material containing following weight/mass percentage composition：Chinese violet trunk cell extract 3%, grape seed extract 7.5%, rhodiola root
Extract 1.5%, mangosteen extract 4%, pearl powder 15%, asparagus juice 15% and honey 8%.
10. the preparation method of the blackhead facial mask of dispelling of the cell extract of trunk containing Chinese violet according to claim 8 or claim 9, it is special
Sign is, comprises the following steps：
(1) described Chinese violet trunk cell extract, grape seed extract, gadol extract and mangosteen extraction are weighed in proportion
Thing, mix standby；
(2) pearl powder, asparagus juice, honey and appropriate water are tuned into paste, the mixture added in step (1), are slowly stirred
Uniformly, the blackhead facial mask of dispelling is produced.
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| CN201711272571.0A CN107837227A (en) | 2017-12-05 | 2017-12-05 | A kind of blackhead skin care item and preparation method thereof of dispelling of the cell extract of trunk containing Chinese violet |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115944574A (en) * | 2023-02-27 | 2023-04-11 | 广州旭妆生物科技有限公司 | Composition for removing blackheads, cosmetic and preparation method |
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|---|---|---|---|---|
| CN105168478A (en) * | 2015-08-28 | 2015-12-23 | 无限极（中国）有限公司 | Skin care product and preparation method thereof |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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2017
- 2017-12-05 CN CN201711272571.0A patent/CN107837227A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168478A (en) * | 2015-08-28 | 2015-12-23 | 无限极（中国）有限公司 | Skin care product and preparation method thereof |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
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| 王建新: "《化妆品植物原料大全》", 30 June 2012 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115944574A (en) * | 2023-02-27 | 2023-04-11 | 广州旭妆生物科技有限公司 | Composition for removing blackheads, cosmetic and preparation method |
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