CN107821055A - A kind of change controlling agent for improving the low nitrogen tolerance of wheat and change prosecutor method - Google Patents
A kind of change controlling agent for improving the low nitrogen tolerance of wheat and change prosecutor method Download PDFInfo
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- CN107821055A CN107821055A CN201711176488.3A CN201711176488A CN107821055A CN 107821055 A CN107821055 A CN 107821055A CN 201711176488 A CN201711176488 A CN 201711176488A CN 107821055 A CN107821055 A CN 107821055A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a kind of change controlling agent for improving the low nitrogen tolerance of wheat and change prosecutor method.Changing controlling agent includes：A) MnSO is contained4、ZnSO4·7H2O, gamma aminobutyric acid and IAA wheat seed immersion preparation；B) under low nitrogen, the water planting of wheat containing humic acid applies preparation with seedling growth nutrient solution or wheat field soil.Wheat seed is soaked 8 10 hours in soaking in preparation first；Before sowing arable land, the pure 40kg/667m of humic acid 30 is spread fertilizer over the fields according to soil fertility earth's surface2, plough immediately, site preparation, sowing processing after seed；Pure N bases amount of application is the 1/2 of conventional base manure amount of application, and the jointing stage of standing up imposes 1/2 3/4 that dosage is conventional base manure amount of application.Said preparation can effectively improve tolerance of the wheat to Low nitrogen stress, Wheat Seedling is grown in low nitrogen environment, and not influence the ultimate output of wheat.
Description
Technical field
The present invention relates to the low nitrogen tolerance technical field of raising wheat, particularly a kind of change for improving the low nitrogen tolerance of wheat
Controlling agent and change prosecutor method.
Background technology
A great number of elements necessary to nitrogen is Growth of Wheat, it is one of important composition element of protein and nucleic acid, it is right
The physiology courses such as photosynthesis of plant have important influence.Under Low nitrogen stress, the growth of crop and photosynthesis can be pressed down
System, so as to influence yield, quality, cause the luminous energy that absorbs superfluous, make ROS metabolism disorders in plant, active oxygen (ROS) and
The lipid peroxidations such as MDA are excessive, disrupting biofilm structure, cause plant finally to lose photosynthetic capacity.In crop production, nitrogen
Element has extremely important effect, and covet yield in agricultural production, makes the amount of application of nitrogenous fertilizer increase year by year.Eighties of last century
Since the eighties, the applying quantity of chemical fertilizer in China is about with annual 1500000 t speed increase.The dosage of wherein chemical nitrogen fertilizer is
Ten thousand t more than 2200, the average amount of nitrogen of unit area that the whole nation there are about more than ten of province have exceeded upper limit 225kg/ generally acknowledged in the world
hm2.However, although nitrogenous fertilizer is largely put into, but nitrogen utilization efficiency is relatively low.The average utilization of world's nitrogenous fertilizer is about 30-50%
(Raun etc., 2002), and China is only 30-35%, some vegetables flakes and high yield area are then lower, and only 10%~20%.
Trace it to its cause and mainly apply nitrogen time and demand of plant growth poor synchronization, excessive nitrogen, low nitrogen are applied in plant growth early stage
The wheat plant absorbability of utilization ratio is limited, causes nutrition to waste, and loss of nitrogen fertilizer rate is more than 45%, simultaneously because NO3 -
The factors such as elution, greenhouse gas emission cause underground water pollution, lake/river eutrophication (eutrophication) and global warming
Etc. environmental problem.
On the other hand, the universal nitrogen stress of China's agricultural land soil, nutrition method of application are that the crop green that people pay close attention to the most is raw
Production and Food Security.The technological approaches to solve the above problems is nothing more than two：First, crop is excavated by biological pathway
The genetic potential of nutrition, the efficient kind of impoverishment tolerant nitrogen is bred as and utilized, using the efficient kind of nitrogen nutrition, excavate crop and efficiently inhale
Receive using nutrient potentiality is one of effective way of raising utilization rate of nitrogen fertilizer；Second, by improving fertilizer quality, fertilizer practice etc.
Agronomic measures improve crop and are resistant to low nitrogen ability, improve crop nitrogen utilization ratio (NUE).Soil fertility level, cultivation step
Deng, all to wheat nitrogen absorb, accumulation and transhipment have a major impact.The supply of wheat nitrogen nutrient depends on root of the crop with absorbing
The absorbability of system and the supply situation of soil mineral nutrient.Thus, on the basis of current improved seeds, it is aided with effective
Agronomic measures can improve the yield and quality of hungry soil wheat, and reduce the dosage of chemical nitrogen fertilizer, improve wheat nitrogen
Utilization ratio (NUE).
Simultaneously with the increase of the chemical fertilizer application time limit, soil organic matter content gradually decreases, physicochemical property, particularly biological
Shape deteriorates year by year, and fertilizer efficiency reduces year by year.At present, to solve these problems, high-quality and efficient nuisanceless fertilizer is developed, changes tradition and applies
Fertilizer custom, the extensive concern of people is caused by new-type fertilizer lifting NUE.
The content of the invention
Instant invention overcomes above-mentioned the deficiencies in the prior art, there is provided a kind of change controlling agent for improving the low nitrogen tolerance of wheat
And change prosecutor method.Said preparation can effectively improve tolerance of the wheat to Low nitrogen stress, allow Wheat Seedling in low azo-cycle
Grown in border, and do not influence the ultimate output of wheat.
The invention provides a kind of change controlling agent for improving the low nitrogen tolerance of wheat, it is characterized in that, it includes：A) Wheat Species
Son immersion preparation (referred to as immersion preparation)；B) wheat water planting seedling growth nutrient solution (abbreviation seedling growth nutrient solution) or wheat
Field soil applies preparation；
The wheat seed soaks preparation, its component and final concentration of：MnSO40.3-0.4g/L, ZnSO4·7H2O
0.4-0.6g/L, γ-aminobutyric acid 0.30-0.60g/L, IAA (indole-3-acetic acid) 20-40mg/L, water surplus；
The wheat water planting is with seedling growth nutrient solution (under the conditions of low nitrogen), its component and final concentration of：Humic acid 0.1-
0.3g/L, pure N<1mM, remaining nutritive element content is the same as Hoagland nutrient solutions；
It is (under the conditions of low nitrogen) that the wheat field soil, which applies preparation,：Humic acid 30-40kg/667m2, humic acid effect
Sustainable 3-4, after applying for the first time, dosage can be reduced to 10-20kg/667m by later year2.Wherein low nitrogen condition is：Base
The pure N dosages of fertilizer are 3-4kg/667m2(the 1/2 of conventional amount used), stand up-the pure N dosages of jointing stage top dressing are 3.5-6.0kg/667m2
(1/2-3/4 of conventional base manure amount of application).
Above-mentioned immersion preparation first can be stored with 100 times of concentrates and (be directly made into 100 times of concentrates when preparing product), use
When be diluted to ultimate density again.Also directly it can prepare and store in solid form, the used time is diluted to ultimate density again.
Present invention also offers a kind of change prosecutor method for improving the low nitrogen tolerance of wheat, it is characterized in that,
1) (or before water planting) seed soaking before sowing
Wheat seed is by (or before water planting) in soaking in preparation immersion 8-10 hours, dosage before sowing：Soaked per 1000ml
Brewed dose of dispensing 2-3kg wheat seed；
Clean after immersion, then directly germinateed in distilled water, cultivated for seedling stage；Or dry at room temperature big to being adapted to
Sowed after field sowing humidity, such as need the long period to preserve, seed should be dried to moisture below 13.5%, and is protected below 13 DEG C
Deposit；
2) seedling stage culture or crop field are applied
Water planting seedling stage cultivates：Seed is germinateed in distilled water after 5d, and wheat seedling is transferred to equipped with the distillation being oxygenated in advance
3d is further cultured in water, to consume the storage nutrition in net seed；Then wheat seedling is inserted equipped with the seedling growth being oxygenated in advance
In the pipette tips box of nutrient solution, (illumination/dark in 5-7 days is cultivated in illumination box：14h/10h, 24/18 DEG C), the next day change once
The seedling growth nutrient solution being oxygenated in advance；Remaining compared with water culture technique.
Apply in crop field：Before sowing arable land, (equivalent) pure humic acid 30-40kg/667m is spread fertilizer over the fields according to soil fertility earth's surface2
(after applying for the first time, dosage can be reduced to 10-20kg/667m by later year2), plough immediately, site preparation, sowing step 1) are handled
Seed afterwards；Pure N amounts of application are the 1/2 of conventional base manure amount of application, i.e. 3-4kg/667m2, stand up-the jointing stage, it is normal to impose pure N
Advise the 1/2-3/4, i.e. 3.5-6kg/667m of base manure amount of application2, remaining same conventional fertilizer application of nutrient amount of application, remaining compared with
Wheat field planting mode.
Preferably, above-mentioned wheat water planting seedling stage, which cultivates, is specially：
1. seedling culture apparatus makes
The pipette tips box of suitable 1ml pipette tips (liquid-transfering gun) is removed into lid, in addition to one side upward, remaining 5 face lucifuge of pipette tips box
Tinfoil paper paper bag is tight；The tip of 1ml pipette tips is cut, tip diameter is slightly larger than wheat seed diameter；
2. seedling is cultivated
Filter paper is spread in culture dish bottom, the above-mentioned seed soaked with immersion preparation is uniformly laid in culture dish, embryo court
Under, appropriate distilled water is added in culture dish, lid is covered with the gauze of sterilizing, distillation water-soaked；
3. seed nutritional is exhausted
Take distillation appropriate amount of water, be oxygenated 3-4h, in the pipette tips box made in pouring into 1., per box 240ml.By 5d children of germinateing
Seedling is transplanted in the pipette tips made, and root exposes from taper end, is inserted in the pipette tips box containing oxygenated distilled water, is cultivated 3- at room temperature
4d, the distilled water being once oxygenated in advance is changed every other day, to consume the storage nutrition in net seed；
4. seedling is cultivated
3. the wheat seedling of middle culture is transferred in the pipette tips box equipped with nutrient solution, per box nutrient solution 240ml；Illumination cultivation
(illumination/dark in 5-7 days is cultivated in case：14h/10h, 24/18 DEG C), the next day change the nutrient solution being once oxygenated in advance.
The component that invention formulation is selected is not to be purely by way of nutrient, more important is：γ-aminobutyric acid and growth
After plain (IAA) is dissolved in water, a slightly acidic environment can be provided for wheat seeds sprouting, being easy to seed sprouting, (wheat is most suitable
Sprouting acid-base property is pH6.4 ± 0.2)；In addition, γ-aminobutyric acid pre-processes with IAA, wheat root lateral root development can be stimulated, is increased
Big root system surface area and N element nutrient absorption amounts；Mn2+And Zn2+It is the activator of Active oxygen scavenging enzymes, it is possible to increase plant cell
The membrane protective enzymatic activity such as middle SOD, POD, CAT, it can remove in Seed Germination because cell membrane disturbs
(perturbations), the physiological activity such as respiration and caused reactive oxygen species (ROS), eucaryotic cell structure is protected, keeps thin
Born of the same parents' physiologically active.Addition humic acid in Wheat Seedling somatomedin (nutrient solution, soil etc.), be advantageous to wheat root development, promote
Enter lateral root, increase root system surface area, improve Absorption of Nitrogen amount.It said components collective effect, can subtract N amounts of application
During few half or so (N reduces tens times during water planting), Wheat Seedling can be with normal growth and development.
Present invention has the advantages that：
1、Mn2+And Zn2+It is the trace element that wheat especially needs, and both general micronutrient levels are low in soil,
Thus Mn in wheat seed2+And Zn2+Accumulation is few, and wheat germination is affected with growth of seedling.Added in invention formulation this two
Kind trace element, wheat seeds sprouting, root system development can be promoted, seed is emerged ahead of time, receives illumination, and it is simultaneously high to carry out photosynthesis
Efficiency absorbs nutrition, is advantageous to wheat population and builds up.
2nd, γ-aminobutyric acid, IAA, the collective effect of humic acid are to promote root system development, are advantageous to plant under the conditions of low nitrogen
Strain Root Absorption nitrogen nutrition, forms strong sprout.
3rd, the most important composition of humic acid soil organic matter, using humic acid be advantageous to improve soil physical chemistry with it is biological
Shape, be advantageous to the absorption of nitrogen and other nutrients in plant pair soil, promote the shape of vine growth and development and yield
Into.
4th, present component is simple, is easy to prepare, and cost is low, is a kind of environmental type controlling agent.Said preparation can have
Effect ground improves tolerance of the wheat to Low nitrogen stress, and alloing Wheat Seedling, (it is left that crop field environment reduces half in low nitrogen environment
The right side, the pure N content of water planting are reduced to below 1mM by 15mM) in grow, and do not influence the ultimate output of wheat.
Embodiment
Embodiment 1
1) preparation controlling agent
Wheat seed soaks preparation, its component and final concentration of：MnSO40.3g/L, ZnSO4·7H2O 0.6g/L, γ-
Aminobutyric acid 0.40g/L, IAA 20mg/L, water surplus；
The wheat water planting is with seedling growth nutrient solution (under the conditions of low nitrogen), its component and final concentration of：Humic acid 0.2g/
L, NO3 -0.25mM, remaining nutritive element content with Hoagland nutrient solutions (will add humic acid in Hoagland nutrient solutions,
And adjust NO3 -Concentration is 0.25mM).
2) soaked seed before water planting
Soaked seed 1 time before water planting, selected wheat seed is soaked 10 hours in soaking in preparation, the immersion preparation per 1000ml
Launch 2.5kg wheat seeds；Distilled water flushing is used after immersion 3-5 times, be put at room temperature in the culture dish for filling appropriate distilled water
Germination；
3) Wheat Seedling growth addition nutrient solution
Seed is germinateed 5d in distilled water, and 10 plants of consistent wheat seedlings of growing way are transferred to equipped with the distilled water being oxygenated in advance
In be further cultured for 3d, to consume the storage nutrition in net seed, then wheat seedling is transferred to and sought equipped with the seedling growth that is oxygenated in advance
In the pipette tips box of nutrient solution；Per box nutrient solution 240ml.In addition to one side upward, remaining 5 face of pipette tips box are tight with lucifuge tinfoil paper paper bag.
Wheat seedling cultivates (illumination/dark in 7 days in illumination box：14h/10h, 24/18 DEG C), the next day change one time of nutrition liquid.Training
Water planting wheat biomass and root traits are determined after supporting 7 days.
Control group：Wheat seed is not soaked seed before water planting, using Hoagland nutrient solutions (0.25mM NO3 -) replace wheat water
Training seedling growth nutrient solution (removes the humic acid in nutrient solution), and remaining is same as above.
The embodiment of the present invention 1 is with influence of the control group to water planting wheat biomass and root traits as shown in table 1-2.From table
1 can be seen that：After the embodiment of the present invention 1 is using the seed soaking of immersion preparation, nutrient solution addition, its overground part, underground part fresh weight have
Obvious increase, increment rate is respectively 9.14%, 10.52%；Its system general length, surface area and lateral root number have obvious increasing
Add, increment rate is respectively 25.40%, 27.02% and 29.85%.
Table 1 changes controlling agent using the influence to water planting wheat biomass (g/10 strains fresh weight)
Table 2 changes controlling agent using the influence to water planting wheat root character
Embodiment 2
1) preparation controlling agent
Wheat seed soaks preparation, its component and final concentration of：MnSO40.4g/L, ZnSO4·7H2O 0.5g/L, γ-
Aminobutyric acid 0.30g/L, IAA 30mg/L, water surplus.
2) soaked seed before Wheat Sowing
Soaked 1 time with immersion preparation before sowing, application rate 10kg/667m2, immersion ratio is：10kg wheat seeds are used
4000ml soaks preparation.Selected wheat seed is soaked 8 hours in preparation, directly dried at room temperature to suitable after immersion
Field seeding humidity.
3) crop field is applied
Before sowing arable land, earth's surface spreads fertilizer over the fields (equivalent) pure humic acid 40kg/667m2, composite fertilizer is spread fertilizer over the fields, is 3.5kg/ containing pure N
667m2；Jointing initial stage, impose pure N 5kg/667m2, remaining same conventional fertilizer application of nutrient amount of application.Other control measures are the same as normal
Rule technology.Mu spike number, mass of 1000 kernel, grain number per spike and yield are counted after harvesting wheat.
Control group 1 (traditional fertilization)：Using traditional fertilization mode (not soaked seed before broadcasting, humic acid is not applied in crop field), full dose
Routine applies nitrogen, spreads fertilizer over the fields composite fertilizer, is 7kg/667m containing pure N2；Jointing initial stage, it is 8kg/667m to impose pure N2；
Control group 2：The change prosecutor method (not soaked seed before broadcasting, crop field is applied and do not use humic acid) of the present invention is not used, is spread
Composite fertilizer is applied, is 3.5kg/667m containing pure N2, jointing initial stage, impose pure N 5kg/667m2。
Influences of the embodiment of the present invention 2 and control group 1-2 to crop field wheat yield is as shown in table 3.As can be seen from Table 3：
Soaked seed using the immersion preparation of the present invention, after increasing humic acid, mu spike number, mass of 1000 kernel, grain number per spike, yield increase, and yield increases
It is 9.92% to add rate.Compared with traditional fertilizing method, change controlling agent of the invention and change prosecutor method, although yield slightly carries
Height, but the amount of application of nitrogen fertilizer of general left and right can be saved, so as to prevent nitrogenous fertilizer excessively to apply water resource pollution brought etc.
Problem.
Table 3 changes application effect of the controlling agent in the Wheat Production of crop field
Claims (7)
1. a kind of change controlling agent for improving the low nitrogen tolerance of wheat, it is characterized in that, it includes：
A) wheat seed immersion preparation；
The wheat seed soaks preparation, its component and final concentration of：MnSO40.3-0.4g/L, ZnSO4·7H2O 0.4-
0.6g/L, γ-aminobutyric acid 0.30-0.60g/L, IAA 20-40mg/L, water surplus；
B) wheat water planting applies preparation with seedling growth nutrient solution or wheat field soil；
The wheat water planting seedling growth nutrient solution, its component and final concentration of：Humic acid 0.1-0.3g/L, pure N<1mM, its
Remaining nutritive element content is the same as Hoagland nutrient solutions；
The wheat field soil is using preparation：Humic acid 10-40kg/667m2。
2. a kind of change controlling agent for improving the low nitrogen tolerance of wheat as claimed in claim 1, it is characterized in that, the humic acid exists
First time amount of application is 30-40kg/667m2, after applying for the first time, later annual amount of application is 10-20kg/667m2。
3. using the change prosecutor method changed controlling agent and improve the low nitrogen tolerance of wheat described in claim 1 or 2, it is characterized in that, pin
To field planting wheat, it comprises the following steps：
1) soaked seed before sowing
Wheat seed is soaked in wheat seed before sowing 8-10 hours are soaked in preparation, dosage is：Per 1000ml wheat seeds
Soak preparation and launch 2-3kg wheat seeds；
Clean after immersion, sowed after drying at room temperature to suitable field seeding humidity；
2) crop field is applied
Apply in crop field：Earth's surface spreads fertilizer over the fields humic acid, plough immediately, site preparation, sowing step 1) processing after seed；Base applies pure N amounts
3-4kg/667m2, stand up-the jointing stage, it is 3.5-6kg/667m to impose pure N amounts2。
4. improve the change prosecutor method of the low nitrogen tolerance of wheat as claimed in claim 3, it is characterized in that, the step 1) is by seed
Dry to moisture below 13.5%, and preserved below 13 DEG C, used again later.
5. using the change prosecutor method changed controlling agent and improve the low nitrogen tolerance of wheat described in claim 1, it is characterized in that,
For water planting wheat, it comprises the following steps：
1) soaked seed before water planting
Wheat seed is soaked in wheat seed before water planting 8-10 hours are soaked in preparation, dosage is：Per 1000ml wheat seeds
Soak preparation and launch 2-3kg wheat seeds；
Clean after immersion, then directly germinateed in distilled water, cultivated for seedling stage；
2) seedling stage cultivates
Water planting seedling stage cultivates：Seed is germinateed in distilled water after 5d, and wheat seedling is transferred to equipped with the distilled water being oxygenated in advance
3-4d is further cultured for, to consume the storage nutrition in net seed；Then wheat seedling is inserted and used equipped with the wheat water planting being oxygenated in advance
In the pipette tips box of seedling growth nutrient solution, cultivated 5-7 days in illumination box, the next day change once be oxygenated in advance seedling growth battalion
Nutrient solution.
6. the change prosecutor method of the low nitrogen tolerance of wheat is improved as claimed in claim 5, it is characterized in that, step 2) the illumination training
Support illumination/dark in case：14h/10h, 24/18 DEG C.
7. the change prosecutor method for improving the low nitrogen tolerance of wheat as described in claim 5 or 6, it is characterized in that, the step 2) seedling
Phase culture is specially：
1. seedling culture apparatus makes
The pipette tips box of suitable 1ml pipette tips is removed into lid, in addition to one side upward, remaining 5 face of pipette tips box are tight with lucifuge tinfoil paper paper bag；
The tip of 1ml pipette tips is cut, tip diameter is slightly larger than wheat seed diameter；
2. seedling is cultivated
Filter paper is spread in culture dish bottom, by step 1) processing after seed be uniformly laid in culture dish, embryo down, in culture dish
Distilled water is added, lid is covered with the gauze of sterilizing, distillation water-soaked；
3. seed nutritional is exhausted
Take distilled water, be oxygenated 3-4h, in the pipette tips box made in pouring into 1.；By the sprigging for the 5d that germinates in the rifle made
In head, root exposes from taper end, inserts in the pipette tips box containing oxygenated distilled water, cultivates 3-4d at room temperature, changes every other day once advance
The distilled water of oxygenation, to consume the storage nutrition in net seed；
4. seedling is cultivated
3. the wheat seedling of middle culture is transferred in the pipette tips box equipped with wheat water planting seedling growth nutrient solution；Illumination box
It is middle culture 5-7 days, the next day change the nutrient solution being once oxygenated in advance.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532338A (en) * | 2022-02-16 | 2022-05-27 | 贵州大学 | Rice culture solution containing gamma-aminobutyric acid, application of rice culture solution in nitrogen stress resistance of rice and rice culture method |
| CN114982614A (en) * | 2022-06-13 | 2022-09-02 | 广东省农业科学院农业生物基因研究中心 | Method for water culture of epimedium brevicornum |
| CN115997680A (en) * | 2022-11-07 | 2023-04-25 | 河南省农业科学院粮食作物研究所 | Low nitrogen resistant millet germplasm identification method |
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| CN1077340A (en) * | 1993-03-17 | 1993-10-20 | 南京农业大学 | Plant growth promoter and production technology thereof |
| CN102106258A (en) * | 2009-12-24 | 2011-06-29 | 上海市农业科学院 | Method for improving low-nitrogen tolerance character of wheat crops |
| CN102144442A (en) * | 2010-11-29 | 2011-08-10 | 中国科学院新疆生态与地理研究所 | Treatment method for improving salt resistance of wheat seeds |
| CN102172154A (en) * | 2011-02-25 | 2011-09-07 | 北京市水利科学研究所 | Method for culturing submerged plant seeds |
| CN106576728A (en) * | 2016-11-17 | 2017-04-26 | 甘肃农业大学 | Method for screening varieties with high utilization rate of nitrogen from multiple wheat varieties |
| CN106673748A (en) * | 2015-11-07 | 2017-05-17 | 哈尔滨地德生物科技有限公司 | Organic nutritional energy element of nitrogen fertilizer |
| CN107006355A (en) * | 2017-04-27 | 2017-08-04 | 河南科技大学 | A kind of wheat seedling mill water culture nutrient solution and preparation method thereof |
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2017
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077340A (en) * | 1993-03-17 | 1993-10-20 | 南京农业大学 | Plant growth promoter and production technology thereof |
| CN102106258A (en) * | 2009-12-24 | 2011-06-29 | 上海市农业科学院 | Method for improving low-nitrogen tolerance character of wheat crops |
| CN102144442A (en) * | 2010-11-29 | 2011-08-10 | 中国科学院新疆生态与地理研究所 | Treatment method for improving salt resistance of wheat seeds |
| CN102172154A (en) * | 2011-02-25 | 2011-09-07 | 北京市水利科学研究所 | Method for culturing submerged plant seeds |
| CN106673748A (en) * | 2015-11-07 | 2017-05-17 | 哈尔滨地德生物科技有限公司 | Organic nutritional energy element of nitrogen fertilizer |
| CN106576728A (en) * | 2016-11-17 | 2017-04-26 | 甘肃农业大学 | Method for screening varieties with high utilization rate of nitrogen from multiple wheat varieties |
| CN107006355A (en) * | 2017-04-27 | 2017-08-04 | 河南科技大学 | A kind of wheat seedling mill water culture nutrient solution and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532338A (en) * | 2022-02-16 | 2022-05-27 | 贵州大学 | Rice culture solution containing gamma-aminobutyric acid, application of rice culture solution in nitrogen stress resistance of rice and rice culture method |
| CN114532338B (en) * | 2022-02-16 | 2023-08-22 | 贵州大学 | Rice culture solution containing gamma-aminobutyric acid, application of rice culture solution in nitrogen stress resistance of rice and rice culture method |
| CN114982614A (en) * | 2022-06-13 | 2022-09-02 | 广东省农业科学院农业生物基因研究中心 | Method for water culture of epimedium brevicornum |
| CN115997680A (en) * | 2022-11-07 | 2023-04-25 | 河南省农业科学院粮食作物研究所 | Low nitrogen resistant millet germplasm identification method |
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