CN107812014B - 自体真皮成纤维细胞的剂量单位调配物 - Google Patents
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| HK1205707A1 (en) * | 2012-03-07 | 2015-12-24 | Fibrocell Technologies, Inc. | Topical dermal formulations and methods of personalized treatment of skin |
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| GB201818243D0 (en) * | 2018-11-08 | 2018-12-26 | Gammadelta Therapeutics Ltd | Methods for isolating and expanding cells |
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| US12544407B2 (en) | 2019-04-28 | 2026-02-10 | Fibrobiologics, Inc. | Fibroblast cell therapy for treatment of osteoporosis |
| CN110075125A (zh) * | 2019-05-10 | 2019-08-02 | 成都康景生物科技有限公司 | 一种用于治疗系统性硬化症伴随肢端硬化的脐带间充质干细胞注射液及其制备方法 |
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Family Cites Families (40)
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|---|---|---|---|---|
| US4524065A (en) | 1983-08-04 | 1985-06-18 | Bio-Specifics N.V. | Method for the prevention and treatment of scars with enzymes |
| US4772557A (en) | 1985-11-12 | 1988-09-20 | Washington University | DNA clone of human skin fibroblast collagenase enzyme |
| US5591444A (en) | 1995-07-28 | 1997-01-07 | Isolagen Technologies, Inc. | Use of autologous dermal fibroblasts for the repair of skin and soft tissue defects |
| US20040229203A1 (en) * | 1996-06-14 | 2004-11-18 | Biostore New Zealand Ltd. | Compositions and methods for the preservation of living tissues |
| AU6662698A (en) | 1997-02-20 | 1998-09-09 | Gregory S. Keller | Augmentation and repair of dermal, subcutaneous, and vocal cord tissue defects |
| US7767452B2 (en) | 1997-02-20 | 2010-08-03 | Kleinsek Don A | Tissue treatments with adipocyte cells |
| US6110459A (en) | 1997-05-28 | 2000-08-29 | Mickle; Donald A. G. | Transplants for myocardial scars and methods and cellular preparations |
| US6150505A (en) * | 1997-09-19 | 2000-11-21 | Hadasit Medical Research Services & Development Ltd. | Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII |
| US7115417B1 (en) * | 1998-05-01 | 2006-10-03 | Chancellor Michael B | Soft tissue and bone augmentation and bulking utilizing muscle-derived progenito compositions, and treatments thereof |
| US6432710B1 (en) | 1998-05-22 | 2002-08-13 | Isolagen Technologies, Inc. | Compositions for regenerating tissue that has deteriorated, and methods for using such compositions |
| WO2000073418A2 (en) | 1999-05-28 | 2000-12-07 | Pacgen Technologies Llc | A method of using autologous fibroblasts to promote healing of wounds and fistulas |
| US6733530B1 (en) | 1999-08-02 | 2004-05-11 | Eric Sun-Yin Chan | Material and method for engraftment of a composite biocompatible skin graft on the neodermis of artificial skin |
| US20080286242A2 (en) | 1999-11-05 | 2008-11-20 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including mesenchymal cells |
| US7799325B2 (en) | 1999-11-05 | 2010-09-21 | Kleinsek Donald A | Removal of hypertrophic scars |
| US20040101959A1 (en) | 2002-11-21 | 2004-05-27 | Olga Marko | Treatment of tissue with undifferentiated mesenchymal cells |
| US7186557B2 (en) | 2003-06-13 | 2007-03-06 | Isolagen Technologies, Inc. | Methods of producing neurons |
| WO2005001080A2 (en) | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Postpartum-derived cells for use in treatment of disease of the heart and circulatory system |
| JP2007520259A (ja) * | 2003-11-10 | 2007-07-26 | シンフォニー メディカル, インコーポレイテッド | 心房細動患者における心室速度を制御するための方法 |
| AU2006251004C1 (en) * | 2005-05-26 | 2013-05-23 | Intercytex Limited | Tissue repair using allogenic dermal fibroblasts |
| KR20080087148A (ko) * | 2006-01-04 | 2008-09-30 | 두-쿱 테크놀로지스 리미티드 | 저온보호 조성물 및 그 이용방법 |
| US20070264239A1 (en) * | 2006-05-10 | 2007-11-15 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Isolation of pericytes |
| GB2440908A (en) * | 2006-05-25 | 2008-02-20 | Intercytex Ltd | Tissue Repair and Augmentation |
| US20070298005A1 (en) * | 2006-06-22 | 2007-12-27 | Marie-Josee Thibault | Injectable composition for treatment of skin defects or deformations |
| KR20070122316A (ko) | 2006-06-26 | 2007-12-31 | (주) 에스바이오메딕스 | 주사용 인체 연조직 충전제 및 이의 제조 방법 |
| WO2008008827A2 (en) * | 2006-07-13 | 2008-01-17 | Medtronic, Inc. | Methods and formulations for optimal local delivery of cell therapy via minimally invasive procedures |
| US8728819B2 (en) * | 2006-08-29 | 2014-05-20 | Fibrocell Technologies, Inc. | Methods for culturing minimally-passaged fibroblasts and uses thereof |
| JP4247333B2 (ja) * | 2006-12-28 | 2009-04-02 | 国立大学法人名古屋大学 | 皮膚組織改善材およびその製造方法 |
| US20100303770A1 (en) | 2006-12-28 | 2010-12-02 | John Maslowski | Methods for culturing dermal cells for treatment of skin injuries such as burns |
| JP2008245583A (ja) * | 2007-03-30 | 2008-10-16 | Yamaguchi Univ | 哺乳動物の神経細胞の精製培養方法とその保存方法 |
| US7960098B2 (en) * | 2007-07-12 | 2011-06-14 | Warsaw Orthoperic, Inc. | Methods and compositions for the preservation of cells and tissues |
| EP2190976A4 (en) | 2007-08-10 | 2010-10-20 | Univ Dayton | METHOD FOR PRODUCING PLURIPOTENTAL STEM CELL LENGTH CELLS |
| JP2009148236A (ja) * | 2007-12-20 | 2009-07-09 | Glycoresearch Kk | 線維芽細胞の細胞識別方法 |
| US20090209456A1 (en) * | 2008-02-19 | 2009-08-20 | Iliana Sweis | Compositions and methods for improving facial and body aesthetics |
| WO2009142717A2 (en) * | 2008-05-19 | 2009-11-26 | President And Fellows Of Harvard College | Methods and products for dedifferentiation of cells |
| US8669048B2 (en) * | 2008-06-24 | 2014-03-11 | Parkinson's Institute | Pluripotent cell lines and methods of use thereof |
| CN102186969A (zh) * | 2008-06-25 | 2011-09-14 | 国家健康与医学研究院 | 由人多能干细胞制备人皮肤替代品的方法 |
| JP2012518409A (ja) * | 2009-02-20 | 2012-08-16 | ベントリア・バイオサイエンス | タンパク質の組み合わせを含有する細胞培養培地 |
| US8240314B2 (en) * | 2009-04-06 | 2012-08-14 | Eden Esthetics, A Professional Nursing Corporation | Method for injecting fillers into the dermis |
| JP5816416B2 (ja) * | 2010-04-23 | 2015-11-18 | 国立大学法人名古屋大学 | 細胞評価装置、インキュベータ、プログラム、および、培養方法 |
| US8529883B2 (en) * | 2010-05-07 | 2013-09-10 | Fibrocell Technologies, Inc. | Dosage unit formulations of autologous dermal fibroblasts |
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| CA2800333A1 (en) | 2011-11-10 |
| US20110274665A1 (en) | 2011-11-10 |
| CA2800333C (en) | 2018-10-30 |
| JP6309268B2 (ja) | 2018-04-11 |
| KR20130060212A (ko) | 2013-06-07 |
| US20130295061A1 (en) | 2013-11-07 |
| EP2566489A1 (en) | 2013-03-13 |
| EP2566489B1 (en) | 2019-04-24 |
| US20230270793A1 (en) | 2023-08-31 |
| US11554142B2 (en) | 2023-01-17 |
| WO2011140323A1 (en) | 2011-11-10 |
| AU2011248067B2 (en) | 2015-03-12 |
| CN107812014A (zh) | 2018-03-20 |
| US8529883B2 (en) | 2013-09-10 |
| JP2013524856A (ja) | 2013-06-20 |
| CN102905711A (zh) | 2013-01-30 |
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