CN107699498B - 一种降解有机磷农药的复合微生物制剂及其制备方法 - Google Patents
一种降解有机磷农药的复合微生物制剂及其制备方法 Download PDFInfo
- Publication number
- CN107699498B CN107699498B CN201710925483.XA CN201710925483A CN107699498B CN 107699498 B CN107699498 B CN 107699498B CN 201710925483 A CN201710925483 A CN 201710925483A CN 107699498 B CN107699498 B CN 107699498B
- Authority
- CN
- China
- Prior art keywords
- microbial preparation
- atcc
- preparation
- composite
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 42
- 230000000813 microbial effect Effects 0.000 title claims abstract description 37
- 239000002131 composite material Substances 0.000 title claims abstract description 27
- 239000003987 organophosphate pesticide Substances 0.000 title claims abstract description 21
- 230000000593 degrading effect Effects 0.000 title claims abstract description 16
- 239000002689 soil Substances 0.000 claims abstract description 30
- 230000001580 bacterial effect Effects 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 150000001875 compounds Chemical class 0.000 claims description 19
- 239000000575 pesticide Substances 0.000 claims description 18
- YASYVMFAVPKPKE-UHFFFAOYSA-N acephate Chemical compound COP(=O)(SC)NC(C)=O YASYVMFAVPKPKE-UHFFFAOYSA-N 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- RLBIQVVOMOPOHC-UHFFFAOYSA-N parathion-methyl Chemical compound COP(=S)(OC)OC1=CC=C([N+]([O-])=O)C=C1 RLBIQVVOMOPOHC-UHFFFAOYSA-N 0.000 claims description 11
- AMFGTOFWMRQMEM-UHFFFAOYSA-N triazophos Chemical compound N1=C(OP(=S)(OCC)OCC)N=CN1C1=CC=CC=C1 AMFGTOFWMRQMEM-UHFFFAOYSA-N 0.000 claims description 11
- 241000588624 Acinetobacter calcoaceticus Species 0.000 claims description 9
- 241000228245 Aspergillus niger Species 0.000 claims description 9
- 241000588915 Klebsiella aerogenes Species 0.000 claims description 9
- 241000589776 Pseudomonas putida Species 0.000 claims description 9
- 244000057717 Streptococcus lactis Species 0.000 claims description 9
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 9
- 229940092559 enterobacter aerogenes Drugs 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 239000010902 straw Substances 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 240000003183 Manihot esculenta Species 0.000 claims description 5
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 5
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- 235000019764 Soybean Meal Nutrition 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 235000013379 molasses Nutrition 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 4
- 239000004455 soybean meal Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 229960004793 sucrose Drugs 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 4
- 230000008485 antagonism Effects 0.000 abstract description 2
- 230000015556 catabolic process Effects 0.000 description 13
- 238000006731 degradation reaction Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000005747 Chlorothalonil Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003993 organochlorine pesticide Substances 0.000 description 2
- 239000000447 pesticide residue Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
组别 | 实施例2组 | 对照组1 | 对照组2 | 对照组3 | 对照组4 | 对照组5 |
乙酰甲胺磷 | 4.9 | 35.3 | 28.1 | 46.2 | 20.9 | 51.4 |
三唑磷 | 3.6 | 29.8 | 21.9 | 33.5 | 27.2 | 40.6 |
甲基对硫磷 | 5.8 | 43.4 | 35.1 | 46.7 | 30.3 | 53.5 |
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710925483.XA CN107699498B (zh) | 2017-10-04 | 2017-10-04 | 一种降解有机磷农药的复合微生物制剂及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710925483.XA CN107699498B (zh) | 2017-10-04 | 2017-10-04 | 一种降解有机磷农药的复合微生物制剂及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107699498A CN107699498A (zh) | 2018-02-16 |
CN107699498B true CN107699498B (zh) | 2020-09-25 |
Family
ID=61184601
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710925483.XA Active CN107699498B (zh) | 2017-10-04 | 2017-10-04 | 一种降解有机磷农药的复合微生物制剂及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107699498B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108723084B (zh) * | 2018-04-12 | 2020-12-08 | 江苏世邦生物工程科技有限公司 | 用于低温土壤修复的微生物制剂及其制备方法和应用 |
CN108342346B (zh) * | 2018-05-14 | 2019-12-17 | 中国科学院生态环境研究中心 | 一株蒙氏假单胞菌及其应用 |
CN109735343B (zh) * | 2018-12-25 | 2021-03-19 | 广东省石油与精细化工研究院 | 一种加速降解土壤中农药残留的组合物 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4447539A (en) * | 1981-01-27 | 1984-05-08 | Sybron Corporation | Microorganism capable of degrading phenolics |
MXPA04010230A (es) * | 2002-04-18 | 2005-06-08 | Celmed Oncology Usa Inc | Profarmacos de peptido deformilasa activada. |
CN100413957C (zh) * | 2006-09-21 | 2008-08-27 | 江苏省农业科学院 | 一种黑曲霉菌株及其应用 |
CN1970731A (zh) * | 2006-12-04 | 2007-05-30 | 山东大学 | 利用黑曲霉制备高效生物铁载体的方法 |
CN101586093A (zh) * | 2007-06-01 | 2009-11-25 | 广东省生态环境与土壤研究所 | 产气肠杆菌及其应用 |
CN101637779A (zh) * | 2008-07-30 | 2010-02-03 | 中国科学院沈阳应用生态研究所 | 一种有机磷降解制剂及其制备方法 |
CN101676225B (zh) * | 2008-09-16 | 2012-10-17 | 佛山市碧沃丰生物科技有限公司 | 一种新型微生物菌剂及其制备方法 |
CN101948893B (zh) * | 2010-09-27 | 2012-06-20 | 郑州奇泓生物科技有限公司 | 连续发酵生产乳酸链球菌素的方法 |
CN102911875A (zh) * | 2012-04-27 | 2013-02-06 | 河南省科学院生物研究所有限责任公司 | 一种多功能土壤修复菌剂的制备方法 |
CN103525703A (zh) * | 2013-10-11 | 2014-01-22 | 上海市农业科学院 | 一种以乙酰甲胺磷为底物的降解菌株的筛选方法 |
CN106434430A (zh) * | 2016-09-07 | 2017-02-22 | 中山市润泽生物科技有限公司 | 一种降解抗生素与农药残留的复合微生物菌剂及其制备与应用 |
CN107032505A (zh) * | 2017-05-24 | 2017-08-11 | 蒋瑞忠 | 用于修复含铅废水的复合菌剂 |
CN107457270B (zh) * | 2017-10-04 | 2020-05-15 | 九江地福来农业科技发展有限公司 | 一种利用生物技术修复有机磷农药污染土壤的方法 |
-
2017
- 2017-10-04 CN CN201710925483.XA patent/CN107699498B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN107699498A (zh) | 2018-02-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Pugliese et al. | Microbial enrichment of compost with biological control agents to enhance suppressiveness to four soil-borne diseases in greenhouse | |
CN102718603B (zh) | 一种复合微生物肥料及其制备方法 | |
CN109679860B (zh) | 一种用于园林绿色废弃物处理的复合菌剂及其制备方法与应用 | |
CN101697737B (zh) | 枯草芽孢杆菌三环唑复配可湿性杀菌粉剂及其应用 | |
CN110156516A (zh) | 一种盐碱地土壤改良剂、制备方法及其应用 | |
CN102199049B (zh) | 一种微生物有机物肥料及其制备方法 | |
CN103467185A (zh) | 生物复合有机菌肥 | |
CN102249753A (zh) | 一种生产多功能生物有机肥的方法及其应用 | |
CN101497541B (zh) | 一种烟草用高效抗病解磷菌肥及其生产方法 | |
CN107699498B (zh) | 一种降解有机磷农药的复合微生物制剂及其制备方法 | |
CN106119155A (zh) | 用于防治花生根腐病的菌株wxx‑2及菌剂 | |
CN107475164B (zh) | 一种复合菌剂及其在降解土壤中拟除虫菊酯类农药残留的用途 | |
CN103396175A (zh) | 一种微生物有机菌肥的制备方法 | |
CN102603371A (zh) | 一种微生物肥料及其制备方法 | |
Shang et al. | Microbial biofertilizer decreases nicotine content by improving soil nitrogen supply | |
CN104774772A (zh) | 一种具有增肥效果的秸秆腐解菌剂及其应用 | |
TWI409242B (zh) | 耐高溫多功能溶磷微生物及其生物肥料製作 | |
CN102838389A (zh) | 一种防治作物土传病害的肥药双效生物有机肥及其制作方法 | |
Pallavi et al. | Commercial microbial products: exploiting beneficial plant-microbe interaction | |
CN106190883A (zh) | 一种土壤改良益生菌组合物及其制备方法 | |
CN104591928A (zh) | 一种具备土壤修复功能的生物有机肥 | |
CN106119154A (zh) | 一种防治连作花生土传真菌病害的菌株及应用 | |
CN108083914A (zh) | 一种多功效复合微生物菌肥及其制备方法 | |
CN107457270B (zh) | 一种利用生物技术修复有机磷农药污染土壤的方法 | |
CN106434442A (zh) | 一种有机磷农药的降解修复剂的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20200903 Address after: 101300 unit 201, floor 2, building 1, yard 2, jiaogezhuang street, Shunyi District, Beijing Applicant after: BEIJING KANGYUANYISHENG BIOTECHNOLOGY Co.,Ltd. Address before: The new town of Fuyang District of Hangzhou City, Zhejiang province 311400 Pan Yan Cun (Hangzhou at Fuyang Agricultural Ecological Agriculture Development Co. Ltd.) Applicant before: Li Ping |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201203 Address after: 1 / F, no.10-1, Jincang Road, Dongling District, Shenyang City, Liaoning Province, 110173 Patentee after: Shenyang Kangyuan Yisheng soil and Water Environment Restoration Research Institute Co.,Ltd. Address before: 101300 unit 201, floor 2, building 1, yard 2, jiaogezhuang street, Shunyi District, Beijing Patentee before: BEIJING KANGYUANYISHENG BIOTECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CP03 | Change of name, title or address |
Address after: Gate 1, No. 27-1 Dongteng 1st Street, Dadong District, Shenyang City, Liaoning Province, 110041 Patentee after: Liaoning Kangyuan Yisheng Biotechnology Co.,Ltd. Country or region after: China Address before: 110173 1st floor, no.10-1, Jincang Road, Dongling District, Shenyang City, Liaoning Province Patentee before: Shenyang Kangyuan Yisheng soil and Water Environment Restoration Research Institute Co.,Ltd. Country or region before: China |
|
CP03 | Change of name, title or address |