CN108723084B - 用于低温土壤修复的微生物制剂及其制备方法和应用 - Google Patents
用于低温土壤修复的微生物制剂及其制备方法和应用 Download PDFInfo
- Publication number
- CN108723084B CN108723084B CN201810329579.4A CN201810329579A CN108723084B CN 108723084 B CN108723084 B CN 108723084B CN 201810329579 A CN201810329579 A CN 201810329579A CN 108723084 B CN108723084 B CN 108723084B
- Authority
- CN
- China
- Prior art keywords
- cold
- microbial
- immobilized
- low
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002689 soil Substances 0.000 title claims abstract description 85
- 230000000813 microbial effect Effects 0.000 title claims abstract description 48
- 238000005067 remediation Methods 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 75
- 101100277015 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) dbp-3 gene Proteins 0.000 claims abstract description 40
- 241000122229 Acinetobacter johnsonii Species 0.000 claims abstract description 32
- 102000030523 Catechol oxidase Human genes 0.000 claims abstract description 28
- 108010031396 Catechol oxidase Proteins 0.000 claims abstract description 28
- 102000003992 Peroxidases Human genes 0.000 claims abstract description 28
- 108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 28
- 239000002131 composite material Substances 0.000 claims abstract description 20
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 10
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims description 41
- 239000003795 chemical substances by application Substances 0.000 claims description 34
- 239000001963 growth medium Substances 0.000 claims description 31
- 238000012258 culturing Methods 0.000 claims description 28
- 150000001875 compounds Chemical class 0.000 claims description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 11
- 229910000278 bentonite Inorganic materials 0.000 claims description 10
- 239000000440 bentonite Substances 0.000 claims description 10
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 241000589291 Acinetobacter Species 0.000 claims description 8
- 239000003463 adsorbent Substances 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 241000207965 Acanthaceae Species 0.000 claims description 7
- 239000012880 LB liquid culture medium Substances 0.000 claims description 7
- 244000017020 Ipomoea batatas Species 0.000 claims description 6
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 239000010902 straw Substances 0.000 claims description 6
- 241000223252 Rhodotorula Species 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 17
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 10
- 230000000052 comparative effect Effects 0.000 abstract description 6
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 abstract description 3
- 239000005839 Tebuconazole Substances 0.000 abstract description 3
- 239000003987 organophosphate pesticide Substances 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 239000000463 material Substances 0.000 description 10
- 239000002808 molecular sieve Substances 0.000 description 10
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 10
- 241001478806 Closterium Species 0.000 description 5
- 241000190932 Rhodopseudomonas Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000191025 Rhodobacter Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 description 1
- 150000003071 polychlorinated biphenyls Chemical group 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Soil Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810329579.4A CN108723084B (zh) | 2018-04-12 | 2018-04-12 | 用于低温土壤修复的微生物制剂及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810329579.4A CN108723084B (zh) | 2018-04-12 | 2018-04-12 | 用于低温土壤修复的微生物制剂及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108723084A CN108723084A (zh) | 2018-11-02 |
CN108723084B true CN108723084B (zh) | 2020-12-08 |
Family
ID=63941405
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810329579.4A Active CN108723084B (zh) | 2018-04-12 | 2018-04-12 | 用于低温土壤修复的微生物制剂及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108723084B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108707558A (zh) * | 2018-04-25 | 2018-10-26 | 江苏世邦生物工程科技有限公司 | 用于修复重金属污染土壤的微生物制剂及其制备方法和应用 |
CN110845024A (zh) * | 2019-12-10 | 2020-02-28 | 清华大学 | 地下水修复模拟实验系统及模拟修复方法 |
CN116656376B (zh) * | 2023-05-31 | 2024-01-05 | 辽宁省微生物科学研究院 | 一种微生物调理剂的制备方法及应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5876634B2 (ja) * | 2008-07-08 | 2016-03-02 | Dowaエコシステム株式会社 | 有機塩素系農薬分解剤及び浄化方法 |
CN101538543B (zh) * | 2009-03-20 | 2011-01-05 | 哈尔滨工业大学 | 低温条件下处理微污染水源水的复合菌剂及其制备方法 |
CN102815792B (zh) * | 2012-04-01 | 2013-12-25 | 浙江绿凯环保科技有限公司 | 一种用于城乡污染河道水体修复的微生物-酶复合制剂及其制备方法 |
CN102787076B (zh) * | 2012-06-18 | 2014-06-04 | 中国科学院微生物研究所 | 一株耐冷玫红假裸囊菌及其在制备冷水纤维素酶中的应用 |
CN103966113A (zh) * | 2013-01-29 | 2014-08-06 | 都江堰惠农生物技术有限责任公司 | 一种农药残留降解复合菌及生产方法 |
CN105524889A (zh) * | 2016-02-03 | 2016-04-27 | 常州市鼎日环保科技有限公司 | 一种修复多环芳烃污染土壤酶制剂的制备方法 |
CN105950172A (zh) * | 2016-06-17 | 2016-09-21 | 战锡林 | 有机磷农药污染土壤修复剂 |
CN106434430A (zh) * | 2016-09-07 | 2017-02-22 | 中山市润泽生物科技有限公司 | 一种降解抗生素与农药残留的复合微生物菌剂及其制备与应用 |
CN107699498B (zh) * | 2017-10-04 | 2020-09-25 | 北京康缘益生生物科技有限公司 | 一种降解有机磷农药的复合微生物制剂及其制备方法 |
CN107413845B (zh) * | 2017-10-09 | 2021-01-29 | 南宁汉和生物科技股份有限公司 | 一种利用微生物制剂降解土壤中拟除虫菊酯类农药残留的方法 |
-
2018
- 2018-04-12 CN CN201810329579.4A patent/CN108723084B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN108723084A (zh) | 2018-11-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106964644B (zh) | 一种有机物污泥土壤修复剂、制备方法及其在污泥消纳中的应用 | |
CN108723084B (zh) | 用于低温土壤修复的微生物制剂及其制备方法和应用 | |
CN109536173B (zh) | 一种同时修复重金属和草甘膦的复合材料及其制备方法 | |
Lin et al. | Biodegradation of tetradecane using Acinetobacter venetianus immobilized on bagasse | |
Zhu et al. | Characteristics of an atrazine degrading bacterium and the construction of a microbial agent for effective atrazine degradation | |
CN108624582A (zh) | 用于土壤修复的微生物制剂 | |
CN108707558A (zh) | 用于修复重金属污染土壤的微生物制剂及其制备方法和应用 | |
CN107759325A (zh) | 一种多功能生物有机肥 | |
CN110591979B (zh) | 农药氧化乐果降解微生物复合菌剂及其制备方法 | |
CN107299066B (zh) | 一种含油污泥的微生物降解液的制备方法以及降解处理方法 | |
Gangola et al. | Enhanced biodegradation of endosulfan by Aspergillus and Trichoderma spp. isolated from an agricultural field of tarai region of Uttarakhand | |
CN107916238B (zh) | 一株百菌清高效降解菌及其在棚室土壤环境中的应用 | |
CN110591974A (zh) | 一种用于皮革污泥干化的嗜热微生物菌剂的制备及使用方法 | |
CN113861997A (zh) | 一种重金属钝化、土壤修复型微生物菌剂及其制备方法 | |
CN111235077B (zh) | 一种修复多氯联苯污染土壤的复合生物制剂 | |
CN108707562B (zh) | 用于修复三氮污染土壤的微生物制剂及其制备方法和应用 | |
CN112110778A (zh) | 一种修复土壤的生物制剂 | |
CN115125173B (zh) | 生物炭基微生物菌剂及其制备方法与应用 | |
Dong et al. | Rapid elimination of dicarboximide fungicides and their metabolite 3, 5-dichloroaniline from soils by immobilized bacterial consortia | |
CN114717222A (zh) | 活性生物材料、制备方法及应用 | |
CN109797123B (zh) | 微生物菌剂及其制备方法和应用 | |
CN113667487A (zh) | 用于生态修复的复合微生物菌剂及其制备方法 | |
CN114409444A (zh) | 以餐前垃圾为原料制备生物有机肥的方法 | |
CN107828692B (zh) | 一株陶厄氏菌及其微生物菌剂制备与应用 | |
CN107338195B (zh) | 降解有机磷农药的木霉菌及其菌剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210723 Address after: 246725 hengbu Industrial Park, hengbu Town, Zongyang County, Tongling City, Anhui Province Patentee after: Anhui Zhongfu Hengyuan Biotechnology Co.,Ltd. Address before: Room 2011, 20 / F, block B, No. 88, pubin Avenue, Jiangpu street, Pukou District, Nanjing City, Jiangsu Province Patentee before: JIANGSU SHIBANG BIOLOGICAL ENGINEERING TECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240606 Address after: Room 2011, 20th Floor, Building B, No. 88 Pubin Avenue, Jiangpu Street, Pukou District, Nanjing City, Jiangsu Province, 210000 Patentee after: JIANGSU SHIBANG BIOLOGICAL ENGINEERING TECHNOLOGY CO.,LTD. Country or region after: China Address before: 246725 hengbu Industrial Park, hengbu Town, Zongyang County, Tongling City, Anhui Province Patentee before: Anhui Zhongfu Hengyuan Biotechnology Co.,Ltd. Country or region before: China |