CN107698313A - The preparation method of the liquid manure of polyoses content in a kind of raising mango - Google Patents
The preparation method of the liquid manure of polyoses content in a kind of raising mango Download PDFInfo
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Abstract
The present invention relates to agricultural technology field, and in particular to a kind of preparation method for improving the liquid manure of polyoses content in mango, including the first step, makes compost：By mass percent, the raw materials such as 1% 5% bacillus globisporuses, 1% 6% Pseudomonas albas, 1% 6% Acinetobacter juniis, 5% 12% grape Aqueous extracts, 6% 13% three-coloured amaranth Aqueous extracts, 5% 15% rotten wood, 10% 15% chicken manures, 10% 18% broomcorn straws, 15% 20% phosphorus mine tailings, 15% 25% maize straws are put into make compost to carry out making compost 5 10 days in pond, you can obtains fermentate；Second step, configure organic liquid manure：By the fermentate made compost in pond and water, nitrogenous fertilizer mixed configuration.This method is simple and easy, finds that organic liquid manure of the application formula can also improve the content of the soluble sugar of mango specifically for its absorption rate of the improvement of P elements, and by research, improves the quality of mango, have higher economic value.
Description
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of preparation side for improving the liquid manure of polyoses content in mango
Method.
【Background technology】
Popular name (the Classification system of mango Shi Mango (Chinese Plants will)：Mangifera indica L.), mango is one
Kind originates in the evergreen megaphanerophyte of Anacardiaceae of India, leaf keratin, alternate；Spend small, polygamy, yellow or faint yellow, into the circular cone of basidixed
Inflorescence.Drupe is big, flattens, long 5-10 centimetres, wide 3-4.5 centimetres, yellow when ripe, sweet, fruit stone is hard.Mango is famous heat
One of band fruit, Mango Fruit contains sugar, protein, crude fibre, the precursor carrotene composition of the vitamin A contained by mango
It is especially high, it is rare in all fruit.Secondly Vitamin C content is not also low.Mineral matter, protein, fat, carbohydrate etc.,
It is its main nutrient composition.Can fruit juice processed, jam, can, pickling, sour and hot pickles and mango milk powder, preserved fruit etc..
Soluble protein and sugar matter is mainly contained in mango polysaccharide.Mango polysaccharide is sweet, slightly sour, mild-natured, the beneficial stomach life of energy
Tianjin, quench the thirst, preventing or arresting vomiting.Purposes：For deficiency of stomach-Yin, thirsty and dry pharynx；Stomach Qi is weak, and dizziness is vomitted inverse.It is that this contains and measured in mango
To the quality for improving can raising mango, reach certain economic benefit.
It is the best way by fertilising to improve the polyoses content in mango.Fertilizer is to aid in the change of plant growth
Compound, the fertilizer type and brand sold on the market are extremely more, can be divided into inorganic fertilizer and organic fertilizer according to composition, according to applicable object
Crop field can be divided into by dividing typically directly is used for soil using fertilizer, family expenses fertilizer, gardens fertilizer, chemical fertilizer, greening fertilizer etc. fertilizer, or sprays
In blade；Fertilizer is to provide nutrient necessary to one or more kinds of plants, improves soil property, water of increasing soil fertility
Flat a kind of material, is one of material base of agricultural production.There is not also a kind of specific aim to improve polysaccharide in mango by retrieval
The preparation method of the liquid manure of content.
It is available by retrieving：
1. Chinese Patent Application No. 201410376500.5, denomination of invention：Mango chemical fertilizer specially, it is by following parts by weight
Raw material be made：Animal wastes 35-50 parts, agricultural crop straw 25-40 parts, dish leaf 15-30 parts, lime 2-10 parts, plant ash 12-
20 parts, Diammonium phosphate (DAP) 18-30 parts, urea 15-30 parts, trbasic zinc phosphate 8-15 parts, potassium chloride 10-20 parts, vermiculite 3-10 parts, biology enzyme
1-8 parts, organic fertilizer fermentation agent 5-12 parts.The present invention passes through using animal wastes, agricultural crop straw and dish leaf as organic fermentation raw material
It is mixed with into one kind with plant ash, Diammonium phosphate (DAP), urea, ammonium phosphate, potassium chloride and vermiculite after fermentation and enzymolysis and had both contained
Machine fertilizer, but the composite chemical fertilizer containing inorganic fertilizer, this kind of fertilizer can meet the nutrition needed for Growth of Mango process, promote the life of mango
It is long, reduce soil hardening, the phenomenon of hardening, moreover it is possible to effectively control dose, reduce cost.But the acidifying to soil is not asked
Topic, also do not improve a certain component content in mango specific aim and convert the quality of raising mango.
2. Chinese Patent Application No. 201710136925.2, denomination of invention：A kind of mango special organic and inorganic fertilizer, invention
A kind of mango special organic and inorganic fertilizer is disclosed, the constituent of the mango special organic and inorganic fertilizer includes decomposed thing
Material, urea, MAP, sulfuric acid signature, calcium superphosphate, borax, zinc sulfate, magnesium sulfate, dolomite dust.The present invention adheres to organic fertilizer
With the fertilization principle of chemical fertilizer compounding application, rich in various nutrient elements such as the nitrogen needed for Growth of Mango process, potassium, phosphorus, calcium
And organic principle, nutrition is balanced, can fully meet the nutrient demand required for Growth of Mango, strengthen the resistance of mango, improves
Fruit yield and quality, there is obvious increasing both production and income to act on；Organic-inorganic constituent of the invention unique can also chesson,
Keep soil from packing together, avoid the accumulation of soil acidification and harmful substance so that soil has a retain water and nutrients ability, and fertility is lasting.
But the element that specific aim absorbs is not strong, does not reach targetedly purpose.
【The content of the invention】
The goal of the invention of the present invention is：For it is above-mentioned the problem of, the present invention, which provides, a kind of improves polyoses content in mango
Liquid manure preparation method, this method is simple and easy, specifically for its absorption rate of the improvement of P elements, and by research hair
Organic liquid manure of existing the application formula can also improve the content of the soluble sugar of mango, improve the quality of mango, have higher
Economic value.
To achieve these goals, the technical solution adopted by the present invention is as follows：
The preparation method of the liquid manure of polyoses content, specifically includes following steps in a kind of raising mango：
The first step, make compost：The raw material of following mass percent is mixed to be put into make compost in pond and made compost, it is described to make compost pond
For rectangle, make compost the bottom in pond and surrounding insertion sets one cavity of formation, cavity top is provided with an aerating mouth and an igniting fills
Put, the volume of the cavity is 5-15 cubic meters, and raw material is put into and made compost in pond, cavity volume 1/2-4/5 is passed through from aerating mouth
Biogas, then will make compost pond closing cover, then starting ignition device lights the biogas in cavity, after biogas combustion is complete
Continue to make compost 5-10 days, you can obtain fermentate；
The raw material has：1%-5% bacillus globisporuses, 1%-6% Pseudomonas albas, 1%-6% Acinetobacter juniis,
5%-12% grapes Aqueous extracts, 6%-13% three-coloured amaranths Aqueous extracts, 5%-15% rotten wood, 10%-15% chicken manures, 10%-18% are high
Fine strain of millet stalk, 15%-20% phosphorus mine tailing, 15%-25% maize straws；
Second step, configure organic liquid manure：By the fermentate made compost in pond and water, nitrogenous fertilizer mixed configuration.
Further, in second step, the fermentate is 2-5 with water, the volume ratio of nitrogenous fertilizer:50-100:1-3.
Further, the time that organic liquid manure uses is mango plantation Post flowering；Dose is 150-200kg/ mus.
Further, the bacillus globisporus, Pseudomonas alba, Acinetobacter junii are also handled by acclimation and screening；
The specific method of the acclimation and screening of three kinds of bacterium is：Bacillus globisporus is put into after being activated in the first culture medium, be located at
30 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:Shaking table culture 20-26 hours are carried out in 10 shaking table, then take shaking table
Strain 1cm after culture2Add in the first new culture medium, positioned at 35 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:5
Shaking table in carry out shaking table culture 20-26 hours, then take the strain 1cm after shaking table culture again2Add another new the
In one culture medium, positioned at 40 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 9:Shaking table culture 20-26 is carried out in 10 shaking table
Hour, the bacterium of survival is then filtered out, the bacterium of survival is added in a first new culture medium, positioned at 45 degrees Celsius, whole
Progress shaking table culture 20-26 hours in the shaking table of nitrogen are passed through, then filter out the circle spore after the bacterium of survival can be tamed
Pseudomonas alba after bacillus or domestication or the Acinetobacter junii after domestication.
Further, the formula of first culture medium is：Potassium dihydrogen phosphate 5g, phyllanthus emblica extract 30g, NaCl 5g, fine jade
Fat 15g, distilled water 1L, pH7.0-7.2.
Further, the phyllanthus emblica extract is prepared by following methods：Phyllanthus emblica is cleaned, smashed after remove seed, so
The alcohol solution dipping of volume mass fraction 75%, 10-20 minutes are added afterwards, and are extracted simultaneously by ultrasonic assistant, ultrasound
The frequency of ripple is 400W-500W, then reclaims ethanol, then adds cellulase, the extract of 0.01 times of volume of extract
The amylase of 0.01 times of volume of protease and extract of 0.01 times of volume is hydrolyzed, and filtrate is then filtrated to get, by filtrate
Via hole diameter is that 1-3 μm of membrane filtration goes filtrate, and then filtrate is passed through after the immersion of 0.01mol/L phosphate buffers
Ion exchange column is filtered, and the flow velocity that filtrate is passed through ion exchange column is 0.1-0.2mL/min, then by filtrate by freezing
Dry or be dried in vacuo or forced air drying obtains phyllanthus emblica extract.
Further, the grape Aqueous extracts use grape pulp to carry out water extraction processing with Grape Skin.
Further, the three-coloured amaranth Aqueous extracts use three-coloured amaranth leaf to carry out water extraction processing.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. the pond of making compost of the application sets a space connected to form cavity in surrounding with bottom, biogas can be passed through, by natural pond
Gas is lighted, and is heated to pond is made compost, and promotes harmful microorganism, the worm's ovum contained in agricultural waste material during fermentation is made compost
Thoroughly killed, but beneficial micropopulation is needed during being made compost, therefore, researcher filters out the application needs
Probioticses, after domestication can high temperature resistant, do not killed by high temperature, in addition, three kinds of bacterium used herein are by domestication
The environment of high temperature anaerobic is adapted to afterwards, and the environmental change during energy specific aim is made compost adapts to, and thalline survival rate is high, and runs into high temperature
The environment of anaerobism has preferably activity, and the quantity in growth period reaches seal point, and then the available phosphorus in energy fast decoupled mixture.
2. using proportioning it is 1%-5% bacillus globisporuses, 1%-6% Pseudomonas albas, 1%-6% Qiong Shi in the application
Acinetobacter calcoaceticus, decompose the P elements in rotten wood, chicken manure, broomcorn straw, phosphorus mine tailing, maize straw, nitrogen, carbon, have
Machine thing etc., and P elements decompose after discharge is active phosphonium ion, active phosphonium ion easily with the iron in soil, alumina
Precipitation reaction or adsorption reaction occur for compound and layer aluminosilicate, calcium carbonate and calcium, iron, aluminium etc., not in time by plant institute
Absorb, cause the place that the utilization rate of P elements is extremely low, compares not by three kinds of bacterium progress phosphorus decomposings and activation phosphonium ion
Reason, the content of rapid available phosphorus improve 2.00mg/kg, and in order to improve the utilization rate of P elements, the method used is a lot, but so far
Non- someone studied the activity for increasing phosphonium ion when making liquid manure, avoided by the material neutralization reaction in soil, and the application
People just from this point, has found by research, with the addition of in formula of the invention mass percent 5%-12% grapes Aqueous extracts,
6%-13% three-coloured amaranth water extraction liquid energies cause active phosphonium ion to have stronger hydrophily, keep the activity of active phosphonium ion, extend
Activity of the active phosphonium ion in soil, the oxalic acid contained in three-coloured amaranth extract solution have the function that to activate phosphonium ion, amaranth well
Dish extract solution has stronger scavenging hydroxyl ability, has preferable oxidation resistance, has well after plant absorption
The polysaccharide material in plant is protected, promotes polyoses content in mango to improve, taste quality improves, but passes through research and find three-coloured amaranth
Active time of the extract solution extension activation phosphonium ion in soil is not grown, therefore passes through countless orthogonal tests, finally found that
Containing substantial amounts of tartaric acid, oxalic acid, pyruvic acid, anthocyanidin in the grape extract of addition, tartaric acid, oxalic acid, pyruvic acid can add
While the activity of the activity, the further phosphonium ion of raising activation of the phosphonium ion of strong firm activation, it can also occur in plant
Biochemical reaction when, strengthen oxidation resistance, will not because of biochemical reaction degradation of cell film, DNA, polysaccharide material etc., two kinds
The combination of extract can reach mutual assistance, the effect of dual guarantee so that the activity of phosphatase reaches after 24 hours
0.0009mg/g, 0.0006mg/g after 48 hours.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
The configuration of first culture medium：Weigh potassium dihydrogen phosphate 5g, phyllanthus emblica extract 30g, NaCl 5g, agar 15g, distillation
Water 1L, adjust pH7.0-7.2.
The grape Aqueous extracts that following examples use are that grape pulp handles to obtain with Grape Skin progress water extraction.
The three-coloured amaranth Aqueous extracts that following examples use use three-coloured amaranth leaf to carry out water extraction and handle what is obtained.
Embodiment 1：
The preparation method of the liquid manure of polyoses content, specifically includes following steps in a kind of raising mango：
The first step, make compost：The raw material of following mass percent is mixed to be put into make compost in pond and made compost, it is described to make compost pond
For rectangle, make compost the bottom in pond and surrounding insertion sets one cavity of formation, cavity top is provided with an aerating mouth and an igniting fills
To put, the volume of the cavity is 5 cubic metres, and raw material is put into and made compost in pond, the biogas of cavity volume 1/2 is passed through from aerating mouth,
Then pond closing will be made compost to cover, then starting ignition device lights the biogas in cavity, continues to macerate after biogas combustion is complete
Fertilizer 5 days, you can obtain fermentate；
The raw material has：
1% bacillus globisporus, 1% Pseudomonas alba, 1% Acinetobacter junii, 5% grape Aqueous extracts, 6% three-coloured amaranth water
Extract, 10% rotten wood, 10% chicken manure, 18% broomcorn straw, 15% phosphorus mine tailing, 25% maize straw；
Second step, configure organic liquid manure：It is 5 by volume ratio:50:1 fermentate made compost in pond mixes with water, nitrogenous fertilizer matches somebody with somebody
Put, applied in mango plantation Post flowering, dose is 150kg/ mus.
Wherein, bacillus globisporus, Pseudomonas alba, Acinetobacter junii are also handled by acclimation and screening；Described three kinds
The specific method of the acclimation and screening of bacterium is：By bacillus globisporus be put into the first culture medium activate after, positioned at 30 degrees Celsius,
It is passed through nitrogen：The volume ratio of oxygen is 3:Shaking table culture is carried out in 10 shaking table 26 hours, then take the strain after shaking table culture
1cm2Add in the first new culture medium, positioned at 35 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:Carried out in 5 shaking table
Shaking table culture 26 hours, the strain 1cm after shaking table culture is then taken again2Add in another first new culture medium, be located at
40 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 9:Shaking table culture is carried out in 10 shaking table 26 hours, then filter out survival
Bacterium, the bacterium of survival is added in a first new culture medium, positioned at 45 degrees Celsius, be all passed through in the shaking table of nitrogen and carry out
Shaking table culture 26 hours, then filter out the bacillus globisporus after the bacterium of survival can be tamed or the false unit cell after domestication
Acinetobacter junii after bacillus or domestication.
Wherein, the phyllanthus emblica extract is prepared by following methods：Phyllanthus emblica is cleaned, smashed after remove seed, Ran Houjia
Enter the alcohol solution dipping of volume mass fraction 75%, 10 minutes, and extracted simultaneously by ultrasonic assistant, the frequency of ultrasonic wave
For 400W, ethanol is then reclaimed, then adds cellulase, the albumen of 0.01 times of volume of extract of 0.01 times of volume of extract
The amylase of 0.01 times of volume of enzyme and extract is hydrolyzed, and is then filtrated to get filtrate, by the filter membrane that filtrate via hole diameter is 1 μm
Filtrate is filtered off, is then filtered the ion exchange column that filtrate is passed through after the immersion of 0.01mol/L phosphate buffers,
The flow velocity that filtrate is passed through ion exchange column is 0.1mL/min, and filtrate then is obtained into phyllanthus emblica extract by forced air drying.
Embodiment 2：
The preparation method of the liquid manure of polyoses content, specifically includes following steps in a kind of raising mango：
The first step, make compost：The raw material of following mass percent is mixed to be put into make compost in pond and made compost, it is described to make compost pond
For rectangle, make compost the bottom in pond and surrounding insertion sets one cavity of formation, cavity top is provided with an aerating mouth and an igniting fills
To put, the volume of the cavity is 15 cubic metres, and raw material is put into and made compost in pond, the biogas of cavity volume 4/5 is passed through from aerating mouth,
Then pond closing will be made compost to cover, then starting ignition device lights the biogas in cavity, continues to macerate after biogas combustion is complete
Fertilizer 10 days, you can obtain fermentate；
The raw material has：
2% bacillus globisporus, 2% Pseudomonas alba, 6% Acinetobacter junii, 12% grape Aqueous extracts, 13% three-coloured amaranth
Aqueous extracts, 5% rotten wood, 15% chicken manure, 10% broomcorn straw, 20% phosphorus mine tailing, 15% maize straw；
Second step, configure organic liquid manure：It is 2 by volume ratio:100:3 fermentate made compost in pond mixes with water, nitrogenous fertilizer
Configuration, applied in mango plantation Post flowering, dose is 200kg/ mus.
Wherein, bacillus globisporus, Pseudomonas alba, Acinetobacter junii are also handled by acclimation and screening；Described three kinds
The specific method of the acclimation and screening of bacterium is：By bacillus globisporus be put into the first culture medium activate after, positioned at 30 degrees Celsius,
It is passed through nitrogen：The volume ratio of oxygen is 3:Shaking table culture is carried out in 10 shaking table 20 hours, then take the strain after shaking table culture
1cm2Add in the first new culture medium, positioned at 35 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:Carried out in 5 shaking table
Shaking table culture 20 hours, the strain 1cm after shaking table culture is then taken again2Add in another first new culture medium, be located at
40 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 9:Shaking table culture is carried out in 10 shaking table 20 hours, then filter out survival
Bacterium, the bacterium of survival is added in a first new culture medium, positioned at 45 degrees Celsius, be all passed through in the shaking table of nitrogen and carry out
Shaking table culture 20 hours, then filter out the bacillus globisporus after the bacterium of survival can be tamed or the false unit cell after domestication
Acinetobacter junii after bacillus or domestication.
Wherein, the phyllanthus emblica extract is prepared by following methods：Phyllanthus emblica is cleaned, smashed after remove seed, Ran Houjia
Enter the alcohol solution dipping of volume mass fraction 75%, 20 minutes, and extracted simultaneously by ultrasonic assistant, the frequency of ultrasonic wave
For 500W, ethanol is then reclaimed, then adds cellulase, the albumen of 0.01 times of volume of extract of 0.01 times of volume of extract
The amylase of 0.01 times of volume of enzyme and extract is hydrolyzed, and is then filtrated to get filtrate, by the filter membrane that filtrate via hole diameter is 3 μm
Filtrate is filtered off, is then filtered the ion exchange column that filtrate is passed through after the immersion of 0.01mol/L phosphate buffers,
The flow velocity that filtrate is passed through ion exchange column is 0.2mL/min, and filtrate then is obtained into phyllanthus emblica extract by vacuum drying.
Embodiment 3：
The preparation method of the liquid manure of polyoses content, specifically includes following steps in a kind of raising mango：
The first step, make compost：The raw material of following mass percent is mixed to be put into make compost in pond and made compost, it is described to make compost pond
For rectangle, make compost the bottom in pond and surrounding insertion sets one cavity of formation, cavity top is provided with an aerating mouth and an igniting fills
To put, the volume of the cavity is 10 cubic metres, and raw material is put into and made compost in pond, the biogas of cavity volume 3/5 is passed through from aerating mouth,
Then pond closing will be made compost to cover, then starting ignition device lights the biogas in cavity, continues to macerate after biogas combustion is complete
Fertilizer 8 days, you can obtain fermentate；
The raw material has：
5% bacillus globisporus, 6% Pseudomonas alba, 2% Acinetobacter junii, 7% grape Aqueous extracts, 10% three-coloured amaranth
Aqueous extracts, 10% rotten wood, 12% chicken manure, 13% broomcorn straw, 16% phosphorus mine tailing, 19% maize straw；
Second step, configure organic liquid manure：It is 3 by volume ratio:90:2 fermentate made compost in pond mixes with water, nitrogenous fertilizer matches somebody with somebody
Put, applied in mango plantation Post flowering, dose is 180kg/ mus.
Wherein, bacillus globisporus, Pseudomonas alba, Acinetobacter junii are also handled by acclimation and screening；Described three kinds
The specific method of the acclimation and screening of bacterium is：By bacillus globisporus be put into the first culture medium activate after, positioned at 30 degrees Celsius,
It is passed through nitrogen：The volume ratio of oxygen is 3:Shaking table culture is carried out in 10 shaking table 22 hours, then take the strain after shaking table culture
1cm2Add in the first new culture medium, positioned at 35 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:Carried out in 5 shaking table
Shaking table culture 23 hours, the strain 1cm after shaking table culture is then taken again2Add in another first new culture medium, be located at
40 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 9:Shaking table culture is carried out in 10 shaking table 24 hours, then filter out survival
Bacterium, the bacterium of survival is added in a first new culture medium, positioned at 45 degrees Celsius, be all passed through in the shaking table of nitrogen and carry out
Shaking table culture 25 hours, then filter out the bacillus globisporus after the bacterium of survival can be tamed or the false unit cell after domestication
Acinetobacter junii after bacillus or domestication.
Wherein, the phyllanthus emblica extract is prepared by following methods：Phyllanthus emblica is cleaned, smashed after remove seed, Ran Houjia
Enter the alcohol solution dipping of volume mass fraction 75%, 15 minutes, and extracted simultaneously by ultrasonic assistant, the frequency of ultrasonic wave
For 450W, ethanol is then reclaimed, then adds cellulase, the albumen of 0.01 times of volume of extract of 0.01 times of volume of extract
The amylase of 0.01 times of volume of enzyme and extract is hydrolyzed, and is then filtrated to get filtrate, by the filter membrane that filtrate via hole diameter is 2 μm
Filtrate is filtered off, is then filtered the ion exchange column that filtrate is passed through after the immersion of 0.01mol/L phosphate buffers,
The flow velocity that filtrate is passed through ion exchange column is 0.15mL/min, and filtrate then is obtained into phyllanthus emblica extract by freeze-drying.
Comparative example 1：
Specific method is same as Example 3, and difference is 5% bacillus globisporus in the raw material, 6% false unit cell bar
Bacterium, 2% Acinetobacter junii do not pass through acclimation.
Comparative example 2：
Specific method is same as Example 3, and difference is that three kinds of bacterium are free of in the raw material, comprises only 6% after domestication
7% Pseudomonas alba after bacillus globisporus, domestication.
Comparative example 3：
Specific method is same as Example 3, and difference is that three kinds of bacterium are free of in the raw material, comprises only 13% after domestication
Acinetobacter junii.
Comparative example 4：
Specific method is same as Example 3, and difference is without 7% grape Aqueous extracts, 10% three-coloured amaranth water in the raw material
Extract, replaced using distilled water.
Comparative example 5：
Specific method is same as Example 3, and difference is that 7% grape Aqueous extracts are free of in the raw material, but is contained
17% three-coloured amaranth Aqueous extracts.
Comparative example 6：
Specific method is same as Example 3, and difference is that phyllanthus emblica extract 30g is replaced using glucose in the first culture medium
Change.
Comparative example 7：
Applied fertilizer using the organic liquid manure of mango on the market.
Experiment：
Phosphorus in rotten wood, chicken manure, broomcorn straw, phosphorus mine tailing, maize straw is detected, phosphorus content is with reference to GB/
T6437-2002 is determined.
Phosphorus content (%) in 1 each raw material of table
| Composition | Rotten wood | Chicken manure | Broomcorn straw | Phosphorus mine tailing | Maize straw |
| Phosphorus | 0.223±0.005 | 1.025±0.025 | 0.225±0.014 | 5.510±0.104 | 0.192±0.007 |
Embodiment 1-3, comparative example 1-6 mode are carried out pouring fertilizer to mango, set up 9 processing regions altogether in trial zone,
Each 30 mango of ecological region planting, each 3 repetitions of processing region, the density of plantation are 150 centimetres of line-spacing, 180 centimetres of spacing in the rows,
1 meter of protection row is reserved between each processing region.
The detection of full phosphorus, rapid available phosphorus is carried out to embodiment 1-3, comparative example 1-6 liquid manure, see the table below 2, full phosphorus is using acid
Molten-molybdenum antimony resistance colorimetric method, rapid available phosphorus use 0.05mol/L NaHCO3- molybdenum antimony resistance colorimetric method.
Table 2
| Full phosphorus (g/kg) | Rapid available phosphorus (mg/kg) | |
| Embodiment 1 | 9.31±0.05 | 7.65±0.25 |
| Embodiment 2 | 8.54±0.07 | 7.11±0.22 |
| Embodiment 3 | 8.92±0.06 | 7.23±0.23 |
| Comparative example 1 | 4.54±0.13 | 4.94±0.22 |
| Comparative example 2 | 6.83±0.11 | 6.71±0.21 |
| Comparative example 3 | 6.31±0.12 | 6.68±0.22 |
| Comparative example 4 | 8.87±0.09 | 7.17±0.11 |
| Comparative example 5 | 8.83±0.10 | 7.12±0.09 |
| Comparative example 6 | 7.05±0.09 | 6.04±0.14 |
| Comparative example 7 | 2.15±0.12 | 5.25±0.22 |
As seen from the above table, the organic liquid manure for configuring to obtain using raw material of the present invention and method is in full phosphorus, available phosphorus contents
With good advantage.It is improper to use our legal system for relatively without comparative example 1 is combined using three kinds of bacterium after domestication
Machine liquid manure is had, the purpose for causing bacterium degraded P elements on the contrary does not reach.Compare using the bacterium after three kinds of domestications, be used alone
Or the phosphorus in the discarded object of combination of two (comparative example 2,3) degraded release rotten wood, chicken manure, broomcorn straw, phosphorus mine tailing, maize straw
The effect of element is slightly poor.The comparative example that compares 6, in the case of the culture medium difference in domestication, the activity of the bacterium of domestication also can
, also can be poor slightly than embodiment 1-3 in the ability of degraded P elements compared to poor.Compared to comparative example 4 or 5, grape water is not added
Extract, three-coloured amaranth Aqueous extracts only add obtained from one of which Aqueous extracts full phosphorus, the content difference of rapid available phosphorus in organic liquid manure
Less, illustrate that grape Aqueous extracts, three-coloured amaranth Aqueous extracts do not have an impact to the middle release P elements of discarded object.
It is right behind 1 day, 2 days, 4 days after fertilising to the detection of soil progress full phosphorus, rapid available phosphorus before being applied fertilizer to mango
Soil carries out the detection of full phosphorus, rapid available phosphorus, see the table below 3, full phosphorus is used using sour molten-molybdenum antimony resistance colorimetric method, rapid available phosphorus
0.05mol/LNaHCO3- molybdenum antimony resistance colorimetric method.
Table 3
As seen from the above table, the suction of obtained organic liquid manure in full phosphorus, rapid available phosphorus is being configured using raw material of the present invention and method
Receiving utilization rate has more superior advantage, particularly in comparative example 4 compared with embodiment 1-3, does not add grape water extraction
Liquid, three-coloured amaranth Aqueous extracts liquid manure in, the activity of P elements is not lasting, and is compared from embodiment 3, comparative example 4, comparative example 5, right
Activity in the liquid manure of ratio 4 not containing grape Aqueous extracts, three-coloured amaranth Aqueous extracts meeting strong influence phosphonium ion, reduces phosphonium ion
Activity so that activation phosphonium ion easily with the iron in soil, aluminum oxide and layer aluminosilicate, calcium carbonate and calcium, iron, aluminium etc.
Generation precipitation reaction or adsorption reaction, are not absorbed by plant in time, and it is extremely low to cause the utilization rate of P elements, and phase
Compared than comparative example 4 with comparative example 5, initial phosphonium ion activity is stronger, is not easy by the material neutralization of soil, but over time
Passage, phosphonium ion activity is not lasting, gradual loss of activity so that behind the 4th day of fertilising, utilization rate is suitable.
Embodiment 1-3, comparative example 1-6 mode are carried out pouring fertilizer to mango, set up 9 processing regions altogether in trial zone,
Each 30 mango of ecological region planting, each 3 repetitions of processing region, the density of plantation are 150 centimetres of line-spacing, 180 centimetres of spacing in the rows,
1 meter of protection row is reserved between each processing region.
To the mango after embodiment 1-3, comparative example 1-6 liquid manure fertilising, apply fertilizer 2 times altogether, be once Post flowering, once
After result, Ripening of Mango Fruit During Storage is then waited, 3 mango are randomly selected in three repetition experimental plots in each region to mango
Sugar content is detected, and each mango detects Data Detection 3 times, anthrone Sulphuric acid Colorimetry soluble sugar content.
The soluble sugar content of table 4/(100g/g)
| First mango | Second mango | 3rd mango | |
| Embodiment 1 | 28.57±2.10 | 28.44±2.20 | 28.51±2.12 |
| Embodiment 2 | 33.78±2.14 | 31.32±2.15 | 33.91±2.02 |
| Embodiment 3 | 29.25±2.22 | 29.12±2.15 | 29.44±2.01 |
| Comparative example 1 | 28.23±2.21 | 28.03±2.22 | 28.15±2.10 |
| Comparative example 2 | 28.14±2.12 | 28.27±2.15 | 28.04±2.16 |
| Comparative example 3 | 28.05±2.15 | 28.37±2.17 | 28.19±2.14 |
| Comparative example 4 | 18.54±2.21 | 18.07±2.07 | 18.15±2.10 |
| Comparative example 5 | 19.92±2.23 | 19.75±2.11 | 19.68±2.12 |
| Comparative example 6 | 27.54±2.15 | 27.68±2.16 | 27.75±2.12 |
| Comparative example 7 | 13.02±2.17 | 13.13±2.15 | 13.24±2.21 |
As seen from the above table, embodiment 1-3 is compared with comparative example 4, comparative example 5, soluble sugar content comparative example 4, contrast
Example 5 reduces 10 (100g/g) left and right compared to embodiment 1-3；Comparative example 4, comparative example 5 compare, the soluble sugar of comparative example 4
Content is lower than the soluble sugar content of comparative example 5, it was demonstrated that addition grape Aqueous extracts, three-coloured amaranth Aqueous extracts collective effect, can be mutual
Help and improve the synthesis of polysaccharide material during biochemical reaction by mango, play a protective role；Compare between embodiment 1-3, it is real
Apply that example 1 is suitable with the soluble sugar content of embodiment 3, the soluble sugar content of embodiment 2 is compared to higher, therefore in configuration liquid manure
It can be regulated and controled by rational ratio, reach different degrees of sugar content.Comparative example 1-3, comparative example 6 compared with Example 3 compared with
Soluble sugar content is more or less the same, it was demonstrated that soluble sugar content with add grape Aqueous extracts, three-coloured amaranth water extraction liquid hold-up it is relevant.Phase
Comparing embodiment 1-3, comparative example 7 compare, and soluble sugar content is far above comparative example 7 in the mango harvested in embodiment 1-3,
Prove that using the liquid manure and the method for fertilising of the application soluble sugar content in mango can be significantly improved.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
- A kind of 1. preparation method for improving the liquid manure of polyoses content in mango, it is characterised in that：Specifically include following steps：The first step, make compost：The raw material of following mass percent is mixed to be put into make compost in pond and made compost, it is described to make compost Chi Weiju Shape, the bottom and surrounding insertion for making compost pond set one cavity of formation, and cavity top is provided with an aerating mouth and an igniter, institute The volume for stating cavity is 5-15 cubic meters, and raw material is put into and made compost in pond, cavity volume 1/2-4/5 natural pond is passed through from aerating mouth Gas, it then will make compost pond closing and cover, then starting ignition device lights the biogas in cavity, continues after biogas combustion is complete Make compost 5-10 days, you can obtain fermentate；The raw material has：1%-5% bacillus globisporuses, 1%-6% Pseudomonas albas, 1%-6% Acinetobacter juniis, 5%- 12% grape Aqueous extracts, 6%-13% three-coloured amaranths Aqueous extracts, 5%-15% rotten wood, 10%-15% chicken manures, 10%-18% sorghum stalks Stalk, 15%-20% phosphorus mine tailing, 15%-25% maize straws；Second step, configure organic liquid manure：By the fermentate made compost in pond and water, nitrogenous fertilizer mixed configuration.
- A kind of 2. preparation method for improving the liquid manure of polyoses content in mango according to claim 1, it is characterised in that： In second step, the fermentate is 2-5 with water, the volume ratio of nitrogenous fertilizer:50-100:1-3.
- A kind of 3. preparation method for improving the liquid manure of polyoses content in mango according to claim 1, it is characterised in that：Institute It is mango plantation Post flowering to state the time that organic liquid manure uses；Dose is 150-200kg/ mus.
- A kind of 4. preparation method for improving the liquid manure of polyoses content in mango according to claim 1, it is characterised in that：Institute Bacillus globisporus, Pseudomonas alba, Acinetobacter junii is stated also to handle by acclimation and screening；The acclimation and screening of three kinds of bacterium Specific method be：By bacillus globisporus be put into the first culture medium activate after, positioned at 30 degrees Celsius, be passed through nitrogen：Oxygen The volume ratio of gas is 3:Shaking table culture 20-26 hours are carried out in 10 shaking table, then take the strain 1cm after shaking table culture2Add In the first new culture medium, positioned at 35 degrees Celsius, be passed through nitrogen：The volume ratio of oxygen is 3:Shaking table culture is carried out in 5 shaking table 20-26 hours, the strain 1cm after shaking table culture is then taken again2Add in another first new culture medium, it is Celsius positioned at 40 Spend, be passed through nitrogen：The volume ratio of oxygen is 9:Shaking table culture 20-26 hours are carried out in 10 shaking table, then filter out survival Bacterium, the bacterium of survival is added in a first new culture medium, shaken positioned at 45 degrees Celsius, whole be passed through in the shaking table of nitrogen Bed culture 20-26 hours, then filter out the bacillus globisporus after the bacterium of survival can be tamed or the vacation after domestication is single Acinetobacter junii after born of the same parents bacillus or domestication.
- A kind of 5. preparation method for improving the liquid manure of polyoses content in mango according to claim 4, it is characterised in that：Institute Stating the formula of the first culture medium is：Potassium dihydrogen phosphate 5g, phyllanthus emblica extract 30g, NaCl 5g, agar 15g, distilled water 1L, pH7.0-7.2。
- A kind of 6. preparation method for improving the liquid manure of polyoses content in mango according to claim 5, it is characterised in that：Institute Stating phyllanthus emblica extract is prepared by following methods：Phyllanthus emblica is cleaned, smashed after remove seed, then adds volume mass fraction 75% alcohol solution dipping, 10-20 minutes, and extracted simultaneously by ultrasonic assistant, the frequency of ultrasonic wave is 400W- 500W, ethanol is then reclaimed, then add cellulase, the protease of 0.01 times of volume of extract of 0.01 times of volume of extract It is hydrolyzed with the amylase of 0.01 times of volume of extract, is then filtrated to get filtrate, by the filter membrane that filtrate via hole diameter is 1-3 μm Filtrate is filtered off, is then filtered the ion exchange column that filtrate is passed through after the immersion of 0.01mol/L phosphate buffers, The flow velocity that filtrate is passed through ion exchange column is 0.1-0.2mL/min, then by filtrate by freeze-drying or vacuum drying or Forced air drying obtains phyllanthus emblica extract.
- A kind of 7. preparation method for improving the liquid manure of polyoses content in mango according to claim 1, it is characterised in that：Institute Stating grape Aqueous extracts uses grape pulp to carry out water extraction processing with Grape Skin.
- A kind of 8. preparation method for improving the liquid manure of polyoses content in mango according to claim 1, it is characterised in that：Institute Stating three-coloured amaranth Aqueous extracts uses three-coloured amaranth leaf to carry out water extraction processing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707028A (en) * | 2018-07-27 | 2018-10-26 | 田东县文设芒果专业合作社 | A kind of preparation method of mango special fertilizer |
| CN108997042A (en) * | 2018-08-15 | 2018-12-14 | 柳州市合联农业有限公司 | A kind of mango special fertilizer material and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104098409A (en) * | 2014-07-31 | 2014-10-15 | 陶坤秀 | Special fertilizer for mangos |
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2017
- 2017-10-09 CN CN201710927983.7A patent/CN107698313A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104098409A (en) * | 2014-07-31 | 2014-10-15 | 陶坤秀 | Special fertilizer for mangos |
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| 冯道主编: "《防沙治沙与生态环境建设实务全书 扬尘、扬沙、沙尘暴防治指南 上》", 31 May 2002, 吉林科学技术出版社 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707028A (en) * | 2018-07-27 | 2018-10-26 | 田东县文设芒果专业合作社 | A kind of preparation method of mango special fertilizer |
| CN108997042A (en) * | 2018-08-15 | 2018-12-14 | 柳州市合联农业有限公司 | A kind of mango special fertilizer material and preparation method thereof |
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