CN107686401A - One kind utilizes microbial manure and preparation method thereof made from yeast fermentation broth - Google Patents
One kind utilizes microbial manure and preparation method thereof made from yeast fermentation broth Download PDFInfo
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- CN107686401A CN107686401A CN201710825690.8A CN201710825690A CN107686401A CN 107686401 A CN107686401 A CN 107686401A CN 201710825690 A CN201710825690 A CN 201710825690A CN 107686401 A CN107686401 A CN 107686401A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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Abstract
The invention provides one kind to utilize microbial manure and preparation method thereof made from yeast fermentation broth, the microbial manure is selected based on saccharomycetes to make fermentation liquid, and add growth regulator and sustained release agent, the zymotic fluid of one side saccharomycete is nutritious, trace element is more, and after culture, obtained tunning is particularly suitable for the growth of beet, the nutriment of abundance is provided for the growth of beet, promote the growth of beet root using growth regulator simultaneously, so as to ensure the yield of beet and sugariness, on the other hand also using the speed of sustained release agent control nutrients release, so as to reduce the number of fertilising, reach single-dose application, continue the effect of getting fat, reduce labor intensity during plantation.
Description
Technical field
The invention belongs to microbial manure technical field, and in particular to one kind utilizes microorganism fertilizer made from yeast fermentation broth
Material and preparation method thereof.
Background technology
Beet, You Ming Chard dishes, biennial herb plant, originates in western European and Southern Coast, west is transplanted to from Sweden
Class's tooth, is a main sugar source beyond tropical sugarcane.Sugar beet originates from Mediterranean, and wild species strand beet is to plant
Train the ancestors of beet.It is about incoming Chinese from Arab countries 1500 or so Christian era.Sugar beet introduces China within 1906.
The cultigen of beet has sugar beet, foliage heet, root garden beet, fodder beet.Wherein sugar beet is most important business
Type, in the modern times, beet sugar accounts for the 2/5 of world's candy output.Therefore yield of the sugar content to its processed goods in raising beet
Have a significant impact with quality, the add-on technology that people use at present is varied, such as sprays Ye Mian Bao, to spray paclobutrazol sweetened
Or although swelling agent, these methods have certain sweetening effect, but the need having spray repeatedly, some sweetening effect unobvious, and
And also there is industrial chemicals or plant hormone, in people food-safe today increasingly paid attention to, such product is not met
The theory of sustainable development, it is therefore desirable to which a kind of safety of preparation has no side effect, the obvious fertilizer of sweetening effect.
Yeast is current microorganism of the annual production more than million tons unique in the world, be widely used in wine brewing, food, in
The fields such as medicine, feed, cosmetics.The primary raw material of yeast industry is caused blackstrap after the sugaring of sugar refinery, and molasses are sugar refinery
Discarded object, including cane molasses and beet molasses, containing about 48% sucrose, using blackstrap produce active dry yeast into
Effectively to utilize blackstrap both at home and abroad, solves one of effective way of sugar plant pollution.Thus, yeast industry is sugar industry industrial chain
In downstream product, be the comprehensive utilization of waste materials of sugar industry product, the reduction to sugar industry pollutant plays very important
Effect.Molasses carry out fermenting and producing yeast after processing, and yeast finished product is obtained after being dried after separating, washing.Fermentation separation process
It is one big technical barrier of world's environmental protection industry that middle discharge, which also has the high-concentration waste water harnessing of carbohydrate, colloid and other organic matters, for
Yeast wastewater processing, external transnational major company's method be not quite similar some using concentration, some using biochemical, but ordinary circumstance is
Processing is discharged into municipal sewage plant's integrated treatment together with municipal sewage afterwards to a certain extent.Such as the yeast enterprise in the U.S., it is
Yeast wastewater is mixed in certain proportion with the city after processing, for agricultural irrigation；European and Brazilian yeast enterprise, be by
Yeast wastewater takes deep or light separately improvement, and feed or fertilizer is made in concentrated water after evaporation and concentration, and fresh water is through biochemical treatment to necessarily
After COD, reprocessed into municipal sewage plant.
" 16 " that China increases the dynamics to environmental protection, particularly party in recent years greatly, propose to move towards infant industry
Change road, it is proposed that " walk a high in technological content, good in economic efficiency, low in resources consumption, low in the pollution of the environment, advantage of human resources
The new industrialization way being not fully exerted ", even more important is required to corporate environment protection, the pollution of yeast wastewater at present is asked
Topic has fundamentally constrained the development of China's yeast industry.
To sum up, how using yeast fermentation waste water rationally can be produced, and can obtains a kind of safety and had no side effect, sweetened effect
The obvious fertilizer of fruit, turns into people's urgent problem to be solved.
The content of the invention
In order to solve the problems, such as that prior art is present, the invention provides one kind to utilize microorganism made from yeast fermentation broth
Fertilizer and preparation method thereof.The microbial manure is selected based on saccharomycetes to make fermentation liquid, and adds growth regulator and sustained release
Agent, the on the one hand growth for beet provides the nutriment of abundance, while promotes the growth of beet root using growth regulator, separately
On the one hand also using the speed of sustained release agent control nutrients release, so as to reduce the number of fertilising, work when reducing plantation is strong
Degree.
It is an object of the invention to provide one kind to utilize microbial manure made from yeast fermentation broth.
Another object of the present invention is to provide the preparation method of the microbial manure.
According to the microbial manure of the present invention, the microbial manure is prepared by the method comprised the following steps：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium and stood under conditions of 28-30 DEG C
Cultivate 24-36 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 5-10%, peptone 0.5-
1%, yeast extract 2-5%, disodium hydrogen phosphate 0.1-0.3%, potassium dihydrogen phosphate 0.1-0.5%, agar 1.5-2.5%, surplus are
Water, pH value 7.0-7.5；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, 28-30 DEG C,
Pressure of fermenting is 0.02-0.025MPa, the condition that throughput is 0.85-1.05v/vmi n, speed of agitator is 120-140rpm
Lower culture 10-12 hours, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 3-8%, bean cake powder 3-5% or corn
Powder 2-4%, peptone 0.5-1%, yeast extract 2-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1-0.5%, surplus are water, pH value 7.0-7.5；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, in 28-31 DEG C, fermentation pressure
5-9 is cultivated under conditions of for 0.02-0.025MPa, throughput be 1.1-1.3v/vmi n, speed of agitator is 110-130rpm
My god, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 7-12%, peptone 0.5-1%, ferment
Female cream 2-5%, urea 3-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate 0.1-0.5%,
Sodium chloride 0.2-0.6%, ferrous sulfate 0.1-0.3%, manganese sulfate 0.1-0.3%, surplus are water, pH value 7.0-7.5；
D, drying and granulating：Sieved after the saccharomycetes to make fermentation liquid that step C is obtained is dried, be prepared into particle diameter less than 1.2mm's
Particle；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 8-12:2-4:1-3, obtain the microbial manure.
According to the microbial manure of the present invention, wherein, inclined-plane activation culture medium, sterilising medium and the fermentation medium
Using being preceding both needed to by sterilization processing, the sterilization processing is：Culture medium is sterilized 12-14 minutes at 121-123 DEG C, then
It is cooled to 25-35 DEG C.
According to the microbial manure of the present invention, wherein, step B, cultivate in seed bacterium solution, by the obtained activation of step A
Saccharomycete is seeded in sterilising medium, and sterilising medium accounts for the 25-45% of round volume.
According to the microbial manure of the present invention, wherein, step C, in fermented and cultured, during fermented and cultured, microscopy fermentation
Material, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain mixed fermentation liquid.
According to the microbial manure of the present invention, wherein, step D, in drying and granulating, in the boiling that EAT is 75-82 DEG C
Rise and dried in bed to moisture≤8%, then cross 0.8-1.2mm screen cloth, obtain particle.
According to the microbial manure of the present invention, wherein, in step E, the growth regulator is diethyl aminoethyl hexanoate, CPPU or green grass or young crops
Fresh element, the sustained release agent are sodium carboxymethylcellulose or chitosan oligosaccharide.
According to the preparation method of the microbial manure of the present invention, comprise the following steps：A, saccharomycete is activated：By yeast starter
Kind is seeded on inclined-plane activation culture medium quiescent culture 24-36 hours, the saccharomycete activated under conditions of 28-30 DEG C；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 5-10%, peptone 0.5-
1%, yeast extract 2-5%, disodium hydrogen phosphate 0.1-0.3%, potassium dihydrogen phosphate 0.1-0.5%, agar 1.5-2.5%, surplus are
Water, pH value 7.0-7.5；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, 28-30 DEG C,
Pressure of fermenting is 0.02-0.025MPa, the condition that throughput 0.85-1.05v/vmin, speed of agitator are 120-140rpm
Lower culture 10-12 hours, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 3-8%, bean cake powder 3-5% or corn
Powder 2-4%, peptone 0.5-1%, yeast extract 2-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1-0.5%, surplus are water, pH value 7.0-7.5；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, in 28-31 DEG C, fermentation pressure
5-9 is cultivated under conditions of being 110-130rpm for 0.02-0.025MPa, throughput 1.1-1.3v/vmin, speed of agitator
My god, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 7-12%, peptone 0.5-1%, ferment
Female cream 2-5%, urea 3-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate 0.1-0.5%,
Sodium chloride 0.2-0.6%, ferrous sulfate 0.1-0.3%, manganese sulfate 0.1-0.3%, surplus are water, pH value 7.0-7.5；
D, drying and granulating：Sieved after the saccharomycetes to make fermentation liquid that step C is obtained is dried, be prepared into particle diameter less than 1.2mm's
Particle；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 8-12:2-4:1-3, obtain the microbial manure.
Preparation in accordance with the present invention, wherein, the inclined-plane activates component system of the culture medium by following percetage by weight
Into：Molasses 6-9%, peptone 0.6-0.9%, yeast extract 3-4%, disodium hydrogen phosphate 0.15-0.25%, potassium dihydrogen phosphate
0.15-0.45%, agar 1.7-2.3%, surplus are water, pH value 7.1-7.4.
Preparation in accordance with the present invention, wherein, the sterilising medium is made up of following components in percentage by weight：Sugar
Sweet 4-7%, bean cake powder 3.5-4.5% or corn flour 2.5-3.5%, peptone 0.6-0.9%, yeast extract 3-4%, magnesium sulfate
0.15-0.25%, disodium hydrogen phosphate 0.15-0.35%, potassium dihydrogen phosphate 0.15-0.45%, surplus are water, pH value 7.1-
7.4。
Preparation in accordance with the present invention, wherein, the fermentation medium is made up of following components in percentage by weight：Sugar
Sweet 8-11%, peptone 0.6-0.9%, yeast extract 2.5-4.5%, urea 3.5-4.5%, magnesium sulfate 0.15-0.25%, phosphoric acid
Disodium hydrogen 0.15-0.35%, potassium dihydrogen phosphate 0.15-0.45%, sodium chloride 0.25-0.55%, ferrous sulfate 0.15-
0.25%, manganese sulfate 0.15-0.25%, surplus are water, pH value 7.1-7.4.
Beneficial effects of the present invention are：
1st, the invention provides one kind to utilize microbial manure made from yeast fermentation broth, and the microbial manure selects ferment
Based on female fermented liquid, and growth regulator and sustained release agent are added, the zymotic fluid of one side saccharomycete is nutritious, micro member
Element is more, and by after above-mentioned culture, obtained tunning is particularly suitable for the growth of beet, and the growth for beet provides
Sufficient nutriment, while promote using growth regulator the growth of beet root, so as to ensure the yield of beet and sugariness, separately
On the one hand also so as to reduce the number of fertilising, reach single-dose application using the speed of sustained release agent control nutrients release, continue getting fat
Effect, reduce labor intensity during plantation.
2nd, the microbial manure, it is graininess, it is convenient to preserve, transport and apply.
3rd, the method for the microbial manure, line activating, culture seed bacterium solution and fermented and cultured, root are entered successively to saccharomycete
According to the physiological property of saccharomycete and the requirement of tunning, from different culture medium and condition of culture, so as to quick, low cost
Ground obtains the microbial manure.
4th, before cultivating saccharomycete, sterilization treatment is carried out to culture medium, to avoid culture medium by living contaminants,
Influence being normally carried out for fermentation.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
One kind utilizes microbial manure made from yeast fermentation broth, method of the microbial manure by comprising the following steps
Prepare：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 28 DEG C
36 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 5%, peptone 1%, yeast extract
2%, disodium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, agar 2.5%, surplus is water, pH value 7.0；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, sterilising medium
Account for the 45% of round volume；28 DEG C, fermentation pressure be 0.025MPa, throughput 0.85v/vmin, speed of agitator
To be cultivated 10 hours under conditions of 140rpm, seed bacterium solution is obtained；
The sterilising medium is made up of following components in percentage by weight：Molasses 8%, bean cake powder 3% or corn flour
2%, peptone 1%, yeast extract 2%, magnesium sulfate 0.3%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, surplus is water,
PH value is 7.0；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 31 DEG C, fermentation pressure
0.02MPa, throughput 1.3v/vmin, speed of agitator are cultivated 9 days under conditions of being 110rpm, during fermented and cultured, mirror
Examine fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 7%, peptone 1%, yeast extract 2%,
Urea 5%, magnesium sulfate 0.1%, disodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.6%, ferrous sulfate
0.1%, manganese sulfate 0.3%, surplus is water, pH value 7.0；
D, drying and granulating：The saccharomycetes to make fermentation liquid that step C is obtained is dried extremely in the ebullated bed that EAT is 82 DEG C
Moisture≤8%, 0.8mm screen cloth is then crossed, be prepared into the particle that particle diameter is less than 0.8mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 12:2:3, obtain the microbial manure.
Wherein, inclined-plane activation culture medium, sterilising medium and fermentation medium are using being preceding both needed to by sterilization
Reason, the sterilization processing are：Culture medium is sterilized 14 minutes at 121 DEG C, is subsequently cooled to 25 DEG C.The growth regulator is chlorine
Pyrrole urea, the sustained release agent are chitosan oligosaccharide.
Embodiment 2
One kind utilizes microbial manure made from yeast fermentation broth, method of the microbial manure by comprising the following steps
Prepare：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 30 DEG C
24 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 10%, peptone 0.5%, ferment
Female cream 5%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, agar 1.5%, surplus are water, pH value 7.5；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, sterilising medium
Account for the 25% of round volume；30 DEG C, fermentation pressure be 0.02MPa, throughput 1.05v/vmin, speed of agitator be
Cultivated 12 hours under conditions of 120rpm, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 3%, bean cake powder 5% or corn flour
4%, peptone 0.5%, yeast extract 5%, magnesium sulfate 0.1%, disodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.1%, surplus is
Water, pH value 7.5；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 28 DEG C, fermentation pressure
0.025MPa, throughput 1.1v/vmin, speed of agitator are cultivated 5 days under conditions of being 130rpm, during fermented and cultured,
Microscopy fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 12%, peptone 0.5%, yeast extract
5%, urea 3%, magnesium sulfate 0.3%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, sodium chloride 0.2%, ferrous sulfate
0.3%, manganese sulfate 0.1%, surplus is water, pH value 7.5；
D, drying and granulating：The saccharomycetes to make fermentation liquid that step C is obtained is dried extremely in the ebullated bed that EAT is 75 DEG C
Moisture≤8%, 1.2mm screen cloth is then crossed, be prepared into the particle that particle diameter is less than 1.2mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 8:4:1, obtain the microbial manure.
Wherein, inclined-plane activation culture medium, sterilising medium and fermentation medium are using being preceding both needed to by sterilization
Reason, the sterilization processing are：Culture medium is sterilized 12 minutes at 123 DEG C, is subsequently cooled to 35 DEG C.The growth regulator be or
Maleic hydrazide, the sustained release agent are sodium carboxymethylcellulose.
Embodiment 3
One kind utilizes microbial manure made from yeast fermentation broth, method of the microbial manure by comprising the following steps
Prepare：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 29 DEG C
30 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 7.5%, peptone 0.75%,
Yeast extract 3.5%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.3%, agar 2%, surplus are water, pH value 7.2；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, sterilising medium
Account for the 35% of round volume；29 DEG C, fermentation pressure be 0.02MPa, throughput 0.95v/vmin, speed of agitator be
Cultivated 11 hours under conditions of 130rpm, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 5.5%, bean cake powder 4% or corn flour
3%, peptone 0.75%, yeast extract 3.5%, magnesium sulfate 0.2%, disodium hydrogen phosphate 0.25%, potassium dihydrogen phosphate 0.3% is remaining
Measure as water, pH value 7.2；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 29 DEG C, fermentation pressure
0.022MPa, throughput 1.2v/vmin, speed of agitator are cultivated 7 days under conditions of being 120rpm, during fermented and cultured,
Microscopy fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 9.5%, peptone 0.75%, yeast
Cream 3.5%, urea 4%, magnesium sulfate 0.2%, disodium hydrogen phosphate 0.25%, potassium dihydrogen phosphate 0.3%, sodium chloride 0.4%, sulfuric acid
Ferrous iron 0.2%, manganese sulfate 0.2%, surplus are water, pH value 7.2；
D, drying and granulating：The saccharomycetes to make fermentation liquid that step C is obtained is dried extremely in the ebullated bed that EAT is 78 DEG C
Moisture≤8%, 1mm screen cloth is then crossed, be prepared into the particle that particle diameter is less than 1mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 10:3:2, obtain the microbial manure.
Wherein, inclined-plane activation culture medium, sterilising medium and fermentation medium are using being preceding both needed to by sterilization
Reason, the sterilization processing are：Culture medium is sterilized 13 minutes at 122 DEG C, is subsequently cooled to 30 DEG C.The growth regulator is amine
Fresh ester, the sustained release agent are chitosan oligosaccharide.
Embodiment 4
One kind utilizes microbial manure made from yeast fermentation broth, method of the microbial manure by comprising the following steps
Prepare：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 30 DEG C
26 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 6%, peptone 0.9%, yeast
Cream 3%, disodium hydrogen phosphate 0.25%, potassium dihydrogen phosphate 0.15%, agar 2.3%, surplus are water, pH value 7.1；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, sterilising medium
Account for the 25% of round volume；30 DEG C, fermentation pressure be 0.02MPa, throughput 1.05v/vmin, speed of agitator be
Cultivated 12 hours under conditions of 120rpm, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 4%, bean cake powder 3.5% or corn flour
2.5%, peptone 0.9%, yeast extract 3%, magnesium sulfate 0.25%, disodium hydrogen phosphate 0.15%, potassium dihydrogen phosphate 0.45% is remaining
Measure as water, pH value 7.1；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 28 DEG C, fermentation pressure
0.025MPa, throughput 1.1v/vmin, speed of agitator are cultivated 5 days under conditions of being 130rpm, during fermented and cultured,
Microscopy fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 11%, peptone 0.6%, yeast extract
4.5%, urea 3.5%, magnesium sulfate 0.25%, disodium hydrogen phosphate 0.15%, potassium dihydrogen phosphate 0.45%, sodium chloride 0.25%,
Ferrous sulfate 0.25%, manganese sulfate 0.15%, surplus are water, pH value 7.4；
D, drying and granulating：The saccharomycetes to make fermentation liquid that step C is obtained is dried extremely in the ebullated bed that EAT is 77 DEG C
Moisture≤8%, 0.9mm screen cloth is then crossed, be prepared into the particle that particle diameter is less than 0.9mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 11:3:2, obtain the microbial manure.
Wherein, inclined-plane activation culture medium, sterilising medium and fermentation medium are using being preceding both needed to by sterilization
Reason, the sterilization processing are：Culture medium is sterilized 12 minutes at 123 DEG C, is subsequently cooled to 35 DEG C.The growth regulator is chlorine
Pyrrole urea, the sustained release agent are sodium carboxymethylcellulose.
Embodiment 5
One kind utilizes microbial manure made from yeast fermentation broth, method of the microbial manure by comprising the following steps
Prepare：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 28 DEG C
36 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 9%, peptone 0.6%, yeast
Cream 4%, disodium hydrogen phosphate 0.15%, potassium dihydrogen phosphate 0.45%, agar 1.7%, surplus are water, pH value 7.4；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, sterilising medium
Account for the 45% of round volume；28 DEG C, fermentation pressure be 0.025MPa, throughput 0.85v/vmin, speed of agitator
To be cultivated 10 hours under conditions of 140rpm, seed bacterium solution is obtained；
The sterilising medium is made up of following components in percentage by weight：Molasses 7%, bean cake powder 4.5% or corn flour
3.5%, peptone 0.6%, yeast extract 4%, magnesium sulfate 0.15%, disodium hydrogen phosphate 0.35%, potassium dihydrogen phosphate 0.15% is remaining
Measure as water, pH value 7.4；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 31 DEG C, fermentation pressure
0.02MPa, throughput 1.3v/vmin, speed of agitator are cultivated 9 days under conditions of being 110rpm, during fermented and cultured, mirror
Examine fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 8%, peptone 0.9%, yeast extract
2.5%, urea 4.5%, magnesium sulfate 0.15%, disodium hydrogen phosphate 0.35%, potassium dihydrogen phosphate 0.15%, sodium chloride 0.55%,
Ferrous sulfate 0.15%, manganese sulfate 0.25%, surplus are water, pH value 7.1；
D, drying and granulating：The saccharomycetes to make fermentation liquid that step C is obtained is dried extremely in the ebullated bed that EAT is 80 DEG C
Moisture≤8%, 1.1mm screen cloth is then crossed, be prepared into the particle that particle diameter is less than 1.1mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator
Weight ratio with sustained release agent is 9:2:2, obtain the microbial manure.
Wherein, inclined-plane activation culture medium, sterilising medium and fermentation medium are using being preceding both needed to by sterilization
Reason, the sterilization processing are：Culture medium is sterilized 14 minutes at 121 DEG C, is subsequently cooled to 25 DEG C.The growth regulator is green grass or young crops
Fresh element, the sustained release agent are sodium carboxymethylcellulose.
Test example
This test example is the manure trial carried out to the microbial manure.
1. experimental design
This experimental example sets 4 treatment groups, and each treatment group is using following processing：
Treatment group 1：Microbial manure 50kg/667m described in embodiment 32, applied with base manure.
Treatment group 2：The microbial manure 50kg/667m of inactivation2, applied with base manure.
Treatment group 3：The other like product 50kg/667m in market2, applied with base manure.
Control group (CK)：Basal dressing.
2. trial crops and detection project
Trial crops：Beet KWS9442, it is bred as by German KWS seeds companys；
Detection project：Root tuber individual plant weight, per mu yield and root tuber sugar content；
3. result of the test
Testing inspection result is as shown in table 1.
Table 1：Result of the test table
| Detection project | Treatment group 1 | Treatment group 2 | Treatment group 3 | Treatment group 4 |
| Root tuber individual plant weight (kg) | 0.95 | 0.89 | 0.81 | 0.75 |
| Per mu yield (kg) | 6219 | 5847 | 5631 | 5389 |
| Root tuber sugar content (%) | 17.1 | 16.3 | 15.8 | 15.2 |
From result of the test, it can be seen that the per mu yield for the treatment of group 1 is compared with treatment group 2, treatment group 3, treatment group 4, respectively
Volume increase 6.36%, 10.4%, 15.4%；Root tuber individual plant weight is respectively increased 6.74%, 17.2%, 26.7%, root tuber sugar content
0.8,1.4 and 1.9 percentage point is respectively increased, illustrates that the microbial manure can significantly improve the root tuber Weight per plant of beet
Amount, per mu yield and root tuber sugar content.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. one kind utilizes microbial manure made from yeast fermentation broth, it is characterised in that the microbial manure is by including following
It is prepared by the method for step：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 28-30 DEG C
24-36 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 5-10%, peptone 0.5-1%, ferment
Female cream 2-5%, disodium hydrogen phosphate 0.1-0.3%, potassium dihydrogen phosphate 0.1-0.5%, agar 1.5-2.5%, surplus are water, pH value
For 7.0-7.5；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, in 28-30 DEG C, fermentation
Pressure is 0.02-0.025MPa, throughput 0.85-1.05v/vmin, speed of agitator are trained under conditions of being 120-140rpm
10-12 hours are supported, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 3-8%, bean cake powder 3-5% or corn flour 2-
4%, peptone 0.5-1%, yeast extract 2-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate
0.1-0.5%, surplus are water, pH value 7.0-7.5；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 28-31 DEG C, fermentation pressure
0.02-0.025MPa, throughput 1.1-1.3v/vmin, speed of agitator are cultivated 5-9 days under conditions of being 110-130rpm,
Obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 7-12%, peptone 0.5-1%, yeast extract
2-5%, urea 3-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate 0.1-0.5%, chlorination
Sodium 0.2-0.6%, ferrous sulfate 0.1-0.3%, manganese sulfate 0.1-0.3%, surplus are water, pH value 7.0-7.5；
D, drying and granulating：Sieved after the saccharomycetes to make fermentation liquid that step C is obtained is dried, be prepared into the particle that particle diameter is less than 1.2mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator ease up
The weight ratio for releasing agent is 8-12:2-4:1-3, obtain the microbial manure.
2. microbial manure according to claim 1, it is characterised in that the inclined-plane activation culture medium, sterilising medium
With fermentation medium using being preceding both needed to by sterilization processing, the sterilization processing is：By culture medium in 121-123 DEG C of sterilizing
12-14 minutes, it is subsequently cooled to 25-35 DEG C.
3. microbial manure according to claim 1, it is characterised in that step B, cultivate in seed bacterium solution, step A is obtained
To the saccharomycete of activation be seeded in sterilising medium, sterilising medium accounts for the 25-45% of round volume.
4. microbial manure according to claim 1, it is characterised in that step C, in fermented and cultured, fermented and cultured process
In, microscopy fermentation materials, when in fermentation materials saccharomycete viable count reach 20,000,000,000/gram more than when, obtain mixed fermentation liquid.
5. microbial manure according to claim 1, it is characterised in that step D, in drying and granulating, be in EAT
Dried in 75-82 DEG C of ebullated bed to moisture≤8%, then cross 0.8-1.2mm screen cloth, obtain particle.
6. microbial manure according to claim 1, it is characterised in that in step E, the growth regulator is that amine is fresh
Ester, CPPU or maleic hydrazide, the sustained release agent are sodium carboxymethylcellulose or chitosan oligosaccharide.
7. a kind of preparation method of microbial manure, it is characterised in that comprise the following steps：
A, saccharomycete is activated：Saccharomycete parent species are seeded on inclined-plane activation culture medium the quiescent culture under conditions of 28-30 DEG C
24-36 hours, the saccharomycete activated；
The inclined-plane activation culture medium is made up of the component of following percetage by weight：Molasses 5-10%, peptone 0.5-1%, ferment
Female cream 2-5%, disodium hydrogen phosphate 0.1-0.3%, potassium dihydrogen phosphate 0.1-0.5%, agar 1.5-2.5%, surplus are water, pH value
For 7.0-7.5；
B, seed bacterium solution is cultivated：The saccharomycete of the obtained activation of step A is seeded in sterilising medium, in 28-30 DEG C, fermentation
Pressure is 0.02-0.025MPa, throughput 0.85-1.05v/vmin, speed of agitator are trained under conditions of being 120-140rpm
10-12 hours are supported, obtain seed bacterium solution；
The sterilising medium is made up of following components in percentage by weight：Molasses 3-8%, bean cake powder 3-5% or corn flour 2-
4%, peptone 0.5-1%, yeast extract 2-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate
0.1-0.5%, surplus are water, pH value 7.0-7.5；
C, fermented and cultured：The seed bacterium solution that step B is obtained is seeded in fermentation medium, is in 28-31 DEG C, fermentation pressure
0.02-0.025MPa, throughput 1.1-1.3v/vmin, speed of agitator are cultivated 5-9 days under conditions of being 110-130rpm,
Obtain saccharomycetes to make fermentation liquid；
The fermentation medium is made up of following components in percentage by weight：Molasses 7-12%, peptone 0.5-1%, yeast extract
2-5%, urea 3-5%, magnesium sulfate 0.1-0.3%, disodium hydrogen phosphate 0.1-0.4%, potassium dihydrogen phosphate 0.1-0.5%, chlorination
Sodium 0.2-0.6%, ferrous sulfate 0.1-0.3%, manganese sulfate 0.1-0.3%, surplus are water, pH value 7.0-7.5；
D, drying and granulating：Sieved after the saccharomycetes to make fermentation liquid that step C is obtained is dried, be prepared into the particle that particle diameter is less than 1.2mm；
E, mix：The particle obtained into step D adds growth regulator and sustained release agent, the particle, growth regulator ease up
The weight ratio for releasing agent is 8-12:2-4:1-3, obtain the microbial manure.
8. preparation method according to claim 7, it is characterised in that activate culture medium by following weight percent in the inclined-plane
Several components are made：Molasses 6-9%, peptone 0.6-0.9%, yeast extract 3-4%, disodium hydrogen phosphate 0.15-0.25%, phosphoric acid
Potassium dihydrogen 0.15-0.45%, agar 1.7-2.3%, surplus are water, pH value 7.1-7.4.
9. preparation method according to claim 7, it is characterised in that the sterilising medium is by following percentage by weight
Component is made：Molasses 4-7%, bean cake powder 3.5-4.5% or corn flour 2.5-3.5%, peptone 0.6-0.9%, yeast extract 3-
4%, magnesium sulfate 0.15-0.25%, disodium hydrogen phosphate 0.15-0.35%, potassium dihydrogen phosphate 0.15-0.45%, surplus are water, pH
It is worth for 7.1-7.4.
10. preparation method according to claim 7, it is characterised in that the fermentation medium is by following percentage by weight
Component be made：Molasses 8-11%, peptone 0.6-0.9%, yeast extract 2.5-4.5%, urea 3.5-4.5%, magnesium sulfate
0.15-0.25%, disodium hydrogen phosphate 0.15-0.35%, potassium dihydrogen phosphate 0.15-0.45%, sodium chloride 0.25-0.55%, sulphur
Acid ferrous 0.15-0.25%, manganese sulfate 0.15-0.25%, surplus are water, pH value 7.1-7.4.
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