CN107668028A - A kind of method for making macro fungi sample - Google Patents
A kind of method for making macro fungi sample Download PDFInfo
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- CN107668028A CN107668028A CN201711015734.7A CN201711015734A CN107668028A CN 107668028 A CN107668028 A CN 107668028A CN 201711015734 A CN201711015734 A CN 201711015734A CN 107668028 A CN107668028 A CN 107668028A
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- glue
- sample
- dropping
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- type crystal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method for making macro fungi sample.It comprises the following steps：1) processing is dried in macro fungi fructification sample；2) the infusion epoxy resin type crystal glue-dropping into mould, obtains bottom glue；Then the macro fungi fructification sample by step 1) processing is placed on bottom glue described in mould, Reperfu- sion at least 2 layers of epoxide resin type crystal glue-dropping, is covered the macro fungi fructification sample, that is, is obtained macro fungi sample.The technique of the present invention is simply easy to make, and demand material is few, higher to sample protectiveness, and obtained sample is attractive in appearance, is easy to displaying to view and admire.
Description
Technical field
It is more particularly to a kind of to be dripped using epoxide resin type crystal the present invention relates to a kind of method for making macro fungi sample
The method that glue makes macro fungi sample, belongs to fungus specimen production field.
Background technology
Macro fungi is colorful, comes in every shape, but due to its easily rot and grow insect pest, after collection storage and
Bigger difficulty in displaying be present.It is general at present to be preserved using dry making sample or dipped into formalin, but dry mark
This easily mildews in long-term preserve and grows insect pest, it is necessary to using chemicals come anti-corrosion and anti-insect, unfriendly to environment；
Other fungus specimen different sizes, the especially less fungus specimen of size its uniformly store and show it is extremely difficult；Formal
Woods solution has certain toxicity, while dipped into formalin can cause decoloring reaction.The problem is solved for fungus specimen
Preserve, scientific research and science popularization displaying are of great significance.
Patent《A kind of preparation method of macro fungi sample》(CN105325404A) a kind of macro fungi sample is disclosed
Preparation method, but its step includes liquid nitrogen flash freezer, vacuum drying etc., it is necessary to specifically equipment such as vacuum drier and liquid nitrogen
Deng material, its application is limited, and lacks outer layer protection for small size fungi, is easily damaged because of extruding；Patent《It is a kind of
Macro fungi Slide processing》(CN104996397A) multiple steps such as cleaning, anti-corrosion, dehydration, plasticizing, embedding, are included,
Its complex process；Liquid used in anti-corrosion pretreatment includes the harmful components such as formaldehyde, is unfavorable for promoting.
The content of the invention
It is an object of the invention to provide a kind of method for making macro fungi sample, the technique of the invention simply side of making
Just, demand material is few, higher to sample protectiveness, and obtained sample is attractive in appearance, is easy to displaying to view and admire.
A kind of method for making macro fungi sample provided by the invention, comprises the following steps：1) it is macro fungi is real
Processing is dried in body；
2) the infusion epoxy resin type crystal glue-dropping into mould, obtains bottom glue；Then by the described big of step 1) processing
Type fungus sporophore is placed on bottom glue described in mould, Reperfu- sion at least 2 layers of epoxide resin type crystal glue-dropping, covers institute
Macro fungi fructification is stated, that is, obtains macro fungi sample.
In the present invention, the macro fungi includes ascomycetes and basidiomycetes macro fungi；
The ascomycetes includes the macro fungi of pyrenomycetes and discomycetes；
The basidiomycetes includes at least one of jelly fungus, club fungi, agaric, non-gill fungus, bolete and false truffle.
In the present invention, the macro fungi is specifically visible《Chinese Macrofungi resource illustrated handbook》(Li Yu, Li Taihui, Yang Zhu
It is good, Tu Liguer, wear to kindly help secure the success of and write, Central Plains peasant publishing house 2015).
Also include tentatively cleaning the fungus sporophore before drying treatment in above-mentioned method, in step 1)
The step of, it is preliminary to clean to remove the impurity such as its surface soil and wood chip.
Also include carrying out the macro fungi fructification of processing in above-mentioned method, after step 1) removing surface and
Contouring, so as to its form and be dimensioned for corresponding mould and be easy to make and improve aesthetic measure；Specific cleaning uses brush
Son, contouring are carried out using scalpel.
In above-mentioned method, prepare the epoxide resin type crystal glue-dropping and comprise the following steps：By component A in AB glue and B
It is at room temperature 2.8~3.2 according to mass ratio after component places 3~4h in 30~45 DEG C of incubators:1 is mixed, is stirred i.e.
.
In above-mentioned method, at least one of the dry use is dried, dries and is freeze-dried method；
The material of the mould is silica gel.
In above-mentioned method, the thickness of the bottom glue can be 0.2~0.8cm, concretely 0.2cm, 0.4cm or 0.2
~0.4cm；
When obtaining the bottom glue, the temperature for irrigating the epoxide resin type crystal glue-dropping can be room temperature and/or 30~45
DEG C, standing time can be 10~20 hours, concretely 12 hours, 10~12 hours, 12~20 hours or 10~18 hours.
In above-mentioned method, on the bottom glue, the epoxide resin type water is irrigated to the macro fungi fructification
The thickness of crystalline substance every layer of glue of drop is 0.2~2cm, concretely 0.4~0.6cm or 0.2~0.8cm.
In above-mentioned method, on the bottom glue, the epoxide resin type water is irrigated to the macro fungi fructification
The temperature of crystalline substance drop glue is room temperature and/or 30~45 DEG C, and standing time is 10~20 hours.
The step of also including in above-mentioned method, in step 2) to the macro fungi sample demoulding, polishing and cleaning；
Each side and drift angle grinding process to the macro fungi fructification after perfusion, improve aesthetics and avoid conduct
Sample scratches spectators when putting on display；
The cleaning is cleaned using clear water to the white powder after polishing, with clean blotting paper or cloth after cleaning
Clean out each surface water droplet of sample.
If there is bubble when irrigating the epoxide resin type crystal glue-dropping in above-mentioned method, in step 2), then remove
The bubble, can specifically clean pin, toothpick etc. be used to choose away bubble；
Covered when the epoxide resin type crystal glue-dropping is irrigated in step 2) using overcover above mould, to prevent dust
Debris drop influence it is attractive in appearance；The overcover includes preservative film and/or newspaper.
In the present invention, the epoxide resin type crystal glue-dropping is the AB glue that a kind of two-component is prepared, and main component is epoxy
Resin, phenmethylol, polyethers ammonia；
The general knowledge that the room temperature is known to the skilled person, it is 20~30 DEG C.
The present invention has advantages below：
The technique of the present invention is simply easy to make, and demand material is few, and finished product is higher to sample protectiveness.The present invention is to sample
Making transparency height, color inhibition, fold resistance is good, morphological plasticity is high, attractive in appearance, adapt to various sizes of fungus specimen and solidification
It is harmless afterwards, the size of different specimens can be carried out unified, effectively suppress to mildew and insect pest in macro fungi Saving specimen
Grow, be easy to store, show and observe, improve the application value of sample.
Brief description of the drawings
Fig. 1 is the flow chart of preparation method of the present invention.
Fig. 2 makes basidiomycetes agarics Singh's cup umbrella sample front, the back side and side photo for the present invention.
Fig. 3 makes basidiomycetes Clavarioid fungi digested tankage color Ramaria stricta sample front, the back side and side photo for the present invention.
Fig. 4 makes the younger sister hickory chick sample front of sac fungus discomycetes six, the back side and side photo for the present invention.
Embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
In following embodiments, room temperature is 25 DEG C.
The making of embodiment 1, Singh's cup umbrella (Singerocybe sp.) epoxy crystal glue-dropping sample
Present case is using Singh's cup umbrella sample as material.Made, comprised the following steps that according to flow as shown in Figure 1：
(1) dried after the Singh's cup umbrella collected progress is tentatively cleaned in 45 DEG C of baking ovens.
(2) from fructification progress surface cleaning is dried obtained by step (1), the impurity such as sample surface soil and wood chip is removed.
(3) after component A in AB glue and B component being placed into 3h in 30 DEG C of incubators, at room temperature according to weight than 2.8:1 is carried out
Mix, the glue thick one layer of 0.2cm in mould, room temperature is placed 12 hours.
(4) sample obtained by step (2) is placed on first layer crystal glue-dropping made in (3), according to institute in (3)
The thick glue of second layer 0.3cm is irrigated after stating ratio glue and pays attention to the putting position of sample.
(5) successively 3 layers of encapsulating, every thickness degree 0.5cm, every layer of gelation condition are put for room temperature on the basis of obtained by step (4)
Put 15 hours, last layer is room temperature 20 hours.
(6) the crystal glue-dropping fungus specimen of shaping will be taken out in the mould after completion step (5), with sander to sample
Seamed edge and vertex are polished, and then the white powder after polishing is cleaned with clear water, with clean water suction after cleaning
Paper or cloth clean out each surface water droplet of sample.
(7) sample obtained by step (6) is numbered and registers storage.
Singh's cup umbrella epoxy crystal glue-dropping sample of the present invention is as shown in Figure 2.As shown in Figure 2, making of the present invention to sample
Transparency height, color inhibition, fold resistance is good, morphological plasticity is high, attractive in appearance.The present invention can effectively suppress in macro fungi Saving specimen
Mildew and grown with insect pest, be easy to store, show and observe.
Embodiment 2, digested tankage color Ramaria stricta (Ramaria ephemeroderma R.H.Petersen＆M.Zang) epoxy water
The making of crystalline substance drop glue sample
Present case is using digested tankage color Ramaria stricta sample as material.Made according to flow as shown in Figure 1, specific steps are such as
Under：
(1) the digested tankage color Ramaria stricta fructification naturally dry that will be collected.
(2) fructification will be dried obtained by step (1) and carries out surface cleaning, remove the impurity such as sample surface soil and by defect
Part scalpel rest and reorganization outward appearance.
(3) 35 DEG C of AB glue is placed 4 hours, according to weight than 3.2:1 carry out with postpone in mould fall one layer of 0.2cm thickness
Glue, room temperature place 12 hours.
(4) sample obtained by step (2) is placed on first layer crystal glue-dropping made in (3), according to institute in (3)
The thick glue of second layer 0.2cm is irrigated after stating ratio glue and pays attention to the putting position of sample.
(5) obtained by the step (4) on the basis of successively 3 layers of encapsulating, per thickness degree 0.6-1.0cm, every layer of gelation condition is room
Temperature is placed 14 hours, and last layer is room temperature 22 hours.
(6) the crystal glue-dropping fungus specimen of shaping will be taken out in the mould after completion step (5), with sander to sample
Seamed edge and vertex are polished, and then the white powder after polishing is cleaned with clear water, with clean water suction after cleaning
Paper or cloth clean out each surface water droplet of sample.
(7) sample obtained by step (6) is numbered and registers storage.
Digested tankage color Ramaria stricta epoxy crystal glue-dropping sample of the present invention is as shown in Figure 3.From the figure 3, it may be seen that the present invention is to sample
Making transparency height, color inhibition, fold resistance is good, morphological plasticity is high, attractive in appearance.The present invention can effectively suppress macro fungi sample guarantor
Mildew in depositing and grown with insect pest, be easy to store, show and observe.
The making of embodiment 3, six younger sister hickory chicks (Morchella sextelata M.Kuo) epoxy crystal glue-dropping sample
Present case is using six younger sister hickory chick samples as material.Made according to flow as shown in Figure 1, specific steps are such as
Under：
(1) the six younger sister's Morchella esculenta (L.) Pers sporophores collected are freeze-dried.
(2) fructification will be dried obtained by step (1) and carries out surface cleaning, remove the debris such as sample surface dirt and by defect
Part carry out outward appearance rest and reorganization with scalpel.
(3) 45 DEG C of AB glue is placed 3.5 hours, according to weight than 3.05:1 carry out with postpone in mould fall one layer
Glue thick 0.4cm, room temperature are placed 12 hours.
(4) sample obtained by step (2) is placed on first layer crystal glue-dropping made in (3), according to institute in (3)
The thick glue of second layer 0.5cm is irrigated after stating ratio glue and pays attention to the putting position of sample.
(5) successively 4 layers of encapsulating, every thickness degree 0.8cm, every layer of gelation condition are put for room temperature on the basis of obtained by step (4)
Put 12 hours, last layer is room temperature 24 hours.
(6) the crystal glue-dropping fungus specimen of shaping will be taken out in the mould after completion step (5), with sander to sample
Seamed edge and vertex are polished, and then the white powder after polishing is cleaned with clear water, with clean water suction after cleaning
Paper or cloth clean out each surface water droplet of sample.
(7) sample obtained by step (6) is numbered and registers storage.
Six younger sister's hickory chick epoxy crystal glue-dropping samples of the invention are as shown in Figure 4.As shown in Figure 4, system of the present invention to sample
Make transparency height, color inhibition, fold resistance is good, morphological plasticity is high, attractive in appearance.The present invention can effectively suppress macro fungi Saving specimen
In mildew and insect pest grow, be easy to store, show and observe.
Claims (10)
1. a kind of method for making macro fungi sample, comprises the following steps：1) processing is dried in macro fungi fructification；
2) the infusion epoxy resin type crystal glue-dropping into mould, obtains bottom glue；Then by the described large-scale true of step 1) processing
Mushroom entity is placed on bottom glue described in mould, Reperfu- sion at least 2 layers of epoxide resin type crystal glue-dropping, is covered described big
Type fungus sporophore, that is, obtain macro fungi sample.
2. according to the method for claim 1, it is characterised in that：In methods described, also wrapped before drying process in step 1)
Include the step of tentatively being cleaned to the macro fungi fructification.
3. method according to claim 1 or 2, it is characterised in that：In methods described, also include to processing after step 1)
The fungus sporophore carry out removing surface and contouring.
4. according to the method any one of claim 1-3, it is characterised in that：Prepare the epoxide resin type crystal glue-dropping
Comprise the following steps：After component A in AB glue and B component are placed into 3~4h in 30~45 DEG C of incubators, at room temperature according to mass ratio
For 2.8~3.2:1 mixed, stir and produce.
5. according to the method any one of claim 1-4, it is characterised in that：It is described dry using drying, dry and cold
Freeze at least one of dry method；
The material of the mould is silica gel.
6. according to the method any one of claim 1-5, it is characterised in that：The thickness of the bottom glue be 0.2~
0.8cm；
When obtaining the bottom glue, the temperature for irrigating the epoxide resin type crystal glue-dropping is room temperature and/or 30~45 DEG C, is placed
Time is 10~20 hours.
7. according to the method any one of claim 1-6, it is characterised in that：On the bottom glue, to described large-scale
Fungus sporophore irrigates the epoxide resin type crystal glue-dropping, and every layer of thickness is 0.2~2cm.
8. the method according to any one of claim 7, it is characterised in that：On the bottom glue, to described large-scale true
The temperature that mushroom entity irrigates the epoxide resin type crystal glue-dropping is room temperature and/or 30~45 DEG C, and standing time is 10~20
Hour.
9. according to the method any one of claim 1-8, it is characterised in that：In methods described, also include in step 2)
The step of to the macro fungi sample demoulding, polishing and cleaning.
10. according to the method any one of claim 1-9, it is characterised in that：In methods described, perfusion institute in step 2)
If there is bubble when stating epoxide resin type crystal glue-dropping, then the bubble is removed；
Covered when the epoxide resin type crystal glue-dropping is irrigated in step 2) using overcover above mould.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711015734.7A CN107668028A (en) | 2017-10-25 | 2017-10-25 | A kind of method for making macro fungi sample |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711015734.7A CN107668028A (en) | 2017-10-25 | 2017-10-25 | A kind of method for making macro fungi sample |
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| CN107668028A true CN107668028A (en) | 2018-02-09 |
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| CN201711015734.7A Pending CN107668028A (en) | 2017-10-25 | 2017-10-25 | A kind of method for making macro fungi sample |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902134A (en) * | 2018-07-04 | 2018-11-30 | 武夏夏 | A kind of production method of medicinal plant epoxy resin permant preparation |
| CN111449059A (en) * | 2020-05-12 | 2020-07-28 | 西南大学 | Orange fruit solidification specimen |
| CN112075414A (en) * | 2020-09-09 | 2020-12-15 | 尹马文 | Specimen packaging method and specimen |
| CN112273376A (en) * | 2020-11-02 | 2021-01-29 | 广西民族师范学院 | Method for preparing Ganoderma applanatum specimen |
| CN113951252A (en) * | 2021-10-29 | 2022-01-21 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for manufacturing macro fungus original state glue dripping specimen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996397A (en) * | 2015-07-03 | 2015-10-28 | 陕西省微生物研究所 | Macrofungi specimen preparation method |
| CN105325404A (en) * | 2015-11-25 | 2016-02-17 | 陕西省微生物研究所 | Manufacturing method of large-size fungus specimen |
-
2017
- 2017-10-25 CN CN201711015734.7A patent/CN107668028A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996397A (en) * | 2015-07-03 | 2015-10-28 | 陕西省微生物研究所 | Macrofungi specimen preparation method |
| CN105325404A (en) * | 2015-11-25 | 2016-02-17 | 陕西省微生物研究所 | Manufacturing method of large-size fungus specimen |
Non-Patent Citations (1)
| Title |
|---|
| 孙广宇 等: "《植物病理学实验技术》", 31 August 2002, 中国农业出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902134A (en) * | 2018-07-04 | 2018-11-30 | 武夏夏 | A kind of production method of medicinal plant epoxy resin permant preparation |
| CN111449059A (en) * | 2020-05-12 | 2020-07-28 | 西南大学 | Orange fruit solidification specimen |
| CN112075414A (en) * | 2020-09-09 | 2020-12-15 | 尹马文 | Specimen packaging method and specimen |
| CN112273376A (en) * | 2020-11-02 | 2021-01-29 | 广西民族师范学院 | Method for preparing Ganoderma applanatum specimen |
| CN112273376B (en) * | 2020-11-02 | 2021-12-14 | 广西民族师范学院 | Method for preparing Ganoderma applanatum specimen |
| CN113951252A (en) * | 2021-10-29 | 2022-01-21 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for manufacturing macro fungus original state glue dripping specimen |
| CN113951252B (en) * | 2021-10-29 | 2023-03-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for manufacturing macro fungus original state glue dripping specimen |
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Application publication date: 20180209 |