CN107646238A - A kind of poison-removing method of sugarcane detoxication tissue culturing seedling - Google Patents
A kind of poison-removing method of sugarcane detoxication tissue culturing seedling Download PDFInfo
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- CN107646238A CN107646238A CN201711055880.2A CN201711055880A CN107646238A CN 107646238 A CN107646238 A CN 107646238A CN 201711055880 A CN201711055880 A CN 201711055880A CN 107646238 A CN107646238 A CN 107646238A
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- Prior art keywords
- sugarcane
- poison
- removing method
- tissue culturing
- culturing seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Botany (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a kind of poison-removing method of sugarcane detoxication tissue culturing seedling, comprises the following steps：Excellent sugarcane plant disease-free, without insect pest is selected, sugarcane plant is removed into sugarcane top, successively rinsing, sterilization and detoxification；Complete to remove the lateral bud of sugarcane plant after above-mentioned steps and/or stem terminal bud carries out sterilization, remove growing point with the scalpel after sterilizing or transfer needle and be inoculated into sterile culture container and cultivated.The present invention fundamentally solves cane seedling by the problem contaminated repeatedly for propagating pathogeny, on this basis, using series of combination tissue culture technology, realize production, breeding and promote tissue cultural seedlings of free, it is numerous to be only used for new varieties expansion for sugar-cane tissue culture seedlings before solving, and the technical barrier of production is not directly applicable, the economic benefit of whole Sugarcane Industry is substantially improved.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of poison-removing method of sugarcane detoxication tissue culturing seedling.
Background technology
Sugarcane, (scientific name：Sanccharum officenarum L) saccharum, perennial tall and big solid draft.Sugarcane stem
It is sturdy tall and big.High 3-5 (- 6) rice of stalk.The southern torrid areas such as TaiWan, China, Fujian, Guangdong, Hainan, Guangxi, Sichuan, Yunnan is wide
General plantation.Sugarcane is adapted to plant in fertile soil, place sunny, summer in the winter temperature difference is big.Sugarcane is temperate zone and tropical farming
Thing, it is the raw material for manufacturing sucrose, and ethanol can be refined as energy substitution product.The whole world has individual country more than 100 to produce sugarcane,
Maximum sugarcane production state is Brazil, India and China.Containing abundant sugar, moisture in sugarcane, also contain newly old to human body
The materials such as the various vitamins being highly profitable, fat, protein, organic acid, calcium, iron are metabolized, are mainly used in refining sugar, epidermis is general
For purple and green two kinds of common colors, also there is red and brown, but it is more rare.
Sugarcane, due to planting repeatedly for many years, is highly prone to seedling biography as the crop that vegetative propagation is carried out with sugarcane stem axillary bud
The dip-dye repeatedly (such as ratoon stunting disease, mosaic disease and smut) of pathogeny is broadcast, causes yield and quality to decline, so as to cause place
The shortening of the root time limit and the degeneration of kind property.The seedling disease that (poison) is propagated in spite of illness is prevented, most economical effective measures are exactly to give birth to
Production, breeding and popularization virus-elimination seedlingses.Under this theoretical direction, partly solves the problems, such as seedling detoxification (tissue culture detoxification before
Seedling and stem section detoxic seedling), but after this tissue culture detoxic seedling seeds into the ground, to pass through triennium can just recover character, arrive
Just the tissue-cultured seedling cane of recovery character can be used for the production into squeezing sugarcane within 4th year.Can not solve disposably to be directly used in into squeezing
The problem of sugarcane produces.
Nursery lot that the also limited amount of stem section detoxic seedling, only footpath spend 3 years expand it is numerous after, can just throw within the 4th year
Enter to enter squeeze sugarcane production in, can not solve the problems, such as disposably to be directly used in and produced into squeezing sugarcane, both detoxic seedlings pass through
After the plantation of 3 years nursery lots, or there is two large problems, first, detoxic seedling has restarted susceptible, simply viral ratio does not have
Have took off poison it is light a bit, more than 1 year will be shortened at least to the perennial root time limit；Second, it can not still break away from the biography for making kind with sugarcane stem
System cultivation mode, every kind of one mu of sugarcane are planted with one ton or so sugarcane stem, cause the waste and the work of carrying and sowing of sugarcane stem
Intensity is very big, so a kind of production technology for the sugarcane detoxication tissue culturing seedling that can solve the problem that above-mentioned two problems of exploitation and this kind of group
The field planting technology of training seedling is very important.Wherein in the production technology of sugarcane detoxication tissue culturing seedling, " sugarcane detoxication tissue culturing seedling
Poison-removing method " be important composition therein part.
The content of the invention
It is an object of the invention to provide a kind of poison-removing method of sugarcane detoxication tissue culturing seedling.
The object of the present invention is achieved like this, a kind of poison-removing method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
Select excellent sugarcane plant disease-free, without insect pest, by sugarcane plant remove sugarcane top, successively rinse, sterilize and
Detoxification；Lateral bud and/or stem terminal bud progress sterilization that above-mentioned steps remove sugarcane plant afterwards are completed, with the dissection after sterilizing
Knife or transfer needle, which are removed growing point and be inoculated into sterile culture container, to be cultivated.
Preferably, described rinsing is that sugarcane plant is rinsed with the clear water of flowing.
Preferably, described sterilization is with 10~40min of washing powder water immersion sugarcane plant.
Preferably, described detoxification includes hot-water detoxified or air high temperature detoxification.
Preferably, it is described it is hot-water detoxified be that sugarcane plant is placed in 0.5~2h of immersion in 50~52 DEG C of warm water.
Preferably, described air high temperature detoxification is to be placed in sugarcane plant in 45 DEG C of hot-air at least 4 weeks.
Preferably, it is in super by lateral bud or stem terminal bud that the lateral bud of the sugarcane plant and/or stem terminal bud, which carry out sterilization,
Carried out disinfection processing with mercuric chloride on net workbench.
Preferably, the growing point refers to the lateral bud of sugarcane plant and/or the top of stem terminal bud.
Preferably, it is provided with explant culture medium in described sterile culture container.
The present invention fundamentally solves the problem repeatedly contaminated of the cane seedling by propagation pathogeny, on this basis,
Using series of combination tissue culture technology, realize production, breeding and promote tissue cultural seedlings of free, sugar-cane tissue culture seedlings can only before solving
It is numerous for new varieties expansion, and the technical barrier of production is not directly applicable, the economic benefit of whole Sugarcane Industry is substantially improved.
Embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not any limitation as in any way,
Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
Embodiment 1
A kind of poison-removing method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
Select excellent sugarcane plant disease-free, without insect pest, by sugarcane plant remove sugarcane top, successively rinse, sterilize and
Detoxification；Lateral bud and/or stem terminal bud progress sterilization that above-mentioned steps remove sugarcane plant afterwards are completed, with the dissection after sterilizing
Knife or transfer needle, which are removed growing point and be inoculated into sterile culture container, to be cultivated.
Preferably, described rinsing is that sugarcane plant is rinsed with the clear water of flowing.
Preferably, described sterilization is with washing powder water immersion sugarcane plant 10min.
Preferably, described detoxification includes hot-water detoxified or air high temperature detoxification.
Preferably, it is described it is hot-water detoxified be that sugarcane plant is placed in 50 DEG C of warm water to soak 2h.
Preferably, described air high temperature detoxification is to be placed in sugarcane plant in 45 DEG C of hot-air at least 4 weeks.
Preferably, it is in super by lateral bud or stem terminal bud that the lateral bud of the sugarcane plant and/or stem terminal bud, which carry out sterilization,
Carried out disinfection processing with mercuric chloride on net workbench.
Preferably, the growing point refers to the lateral bud of sugarcane plant and/or the top of stem terminal bud.
Preferably, it is provided with explant culture medium in described sterile culture container.
Embodiment 2
A kind of poison-removing method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
Select excellent sugarcane plant disease-free, without insect pest, by sugarcane plant remove sugarcane top, successively rinse, sterilize and
Detoxification；Lateral bud and/or stem terminal bud progress sterilization that above-mentioned steps remove sugarcane plant afterwards are completed, with the dissection after sterilizing
Knife or transfer needle, which are removed growing point and be inoculated into sterile culture container, to be cultivated.
Preferably, described rinsing is that sugarcane plant is rinsed with the clear water of flowing.
Preferably, described sterilization is with washing powder water immersion sugarcane plant 40min.
Preferably, described detoxification includes hot-water detoxified or air high temperature detoxification.
Preferably, it is described it is hot-water detoxified be that sugarcane plant is placed in 52 DEG C of warm water to soak 0.5h.
Preferably, described air high temperature detoxification is to be placed in sugarcane plant in 45 DEG C of hot-air at least 4 weeks.
Preferably, it is in super by lateral bud or stem terminal bud that the lateral bud of the sugarcane plant and/or stem terminal bud, which carry out sterilization,
Carried out disinfection processing with mercuric chloride on net workbench.
Preferably, the growing point refers to the lateral bud of sugarcane plant and/or the top of stem terminal bud.
Preferably, it is provided with explant culture medium in described sterile culture container.
Claims (9)
1. a kind of poison-removing method of sugarcane detoxication tissue culturing seedling, it is characterised in that comprise the following steps：
Excellent sugarcane plant disease-free, without insect pest is selected, sugarcane plant is removed into sugarcane top, successively rinsing, sterilization and detoxification；
Complete to remove the lateral bud of sugarcane plant after above-mentioned steps and/or stem terminal bud carry out sterilization, with the scalpel after sterilizing or
Transfer needle, which is removed growing point and is inoculated into sterile culture container, to be cultivated.
2. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that described rinsing is to flow
Dynamic clear water rinses to sugarcane plant.
3. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that described sterilization is with washing
10~40min of clothing powder water immersion sugarcane plant.
4. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that described detoxification includes temperature
Water detoxification or air high temperature detoxification.
5. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 4, it is characterised in that described hot-water detoxified to be
Sugarcane plant is placed in 0.5~2h of immersion in 50~52 DEG C of warm water.
6. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 4, it is characterised in that described air high temperature takes off
Poison is to be placed in sugarcane plant in 45 DEG C of hot-air at least 4 weeks.
7. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the side of the sugarcane plant
It is in being carried out disinfection processing with mercuric chloride on superclean bench by lateral bud or stem terminal bud that bud and/or stem terminal bud, which carry out sterilization,.
8. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the growing point refers to sweet
The lateral bud of sugarcane plant and/or the top of stem terminal bud.
9. the poison-removing method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that described sterile culture is held
Explant culture medium is provided with device.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711055880.2A CN107646238A (en) | 2017-11-01 | 2017-11-01 | A kind of poison-removing method of sugarcane detoxication tissue culturing seedling |
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| CN201711055880.2A CN107646238A (en) | 2017-11-01 | 2017-11-01 | A kind of poison-removing method of sugarcane detoxication tissue culturing seedling |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361458A (en) * | 2008-09-27 | 2009-02-11 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| CN105010137A (en) * | 2015-06-29 | 2015-11-04 | 大新县科学技术情报研究所 | Saccharum officinarum detoxification seedling propagation method |
| US20160360716A1 (en) * | 2014-08-29 | 2016-12-15 | Pioneer Hi-Bred International, Inc. | Methods and devices for creating doubled haploid embryos using oil matrices |
| CN106818471A (en) * | 2017-01-13 | 2017-06-13 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of method of utilization sugarcane health cultivating seeds high yield sugarcane |
-
2017
- 2017-11-01 CN CN201711055880.2A patent/CN107646238A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361458A (en) * | 2008-09-27 | 2009-02-11 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| US20160360716A1 (en) * | 2014-08-29 | 2016-12-15 | Pioneer Hi-Bred International, Inc. | Methods and devices for creating doubled haploid embryos using oil matrices |
| CN105010137A (en) * | 2015-06-29 | 2015-11-04 | 大新县科学技术情报研究所 | Saccharum officinarum detoxification seedling propagation method |
| CN106818471A (en) * | 2017-01-13 | 2017-06-13 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of method of utilization sugarcane health cultivating seeds high yield sugarcane |
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Application publication date: 20180202 |
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