CN107637446A - A kind of method for making cereal organic selenium-rich using artificial culture - Google Patents

A kind of method for making cereal organic selenium-rich using artificial culture Download PDF

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Publication number
CN107637446A
CN107637446A CN201711103477.2A CN201711103477A CN107637446A CN 107637446 A CN107637446 A CN 107637446A CN 201711103477 A CN201711103477 A CN 201711103477A CN 107637446 A CN107637446 A CN 107637446A
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cereal
rich
selenium
organic selenium
culture
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林克鹏
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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Abstract

The invention discloses a kind of method for making cereal organic selenium-rich using artificial culture, after selected cereal is placed in into Selenium-rich nutrient solution culture, it is positioned in incubated room and cultivates, in the different phase of cereal sprout growth, the different intensity of illumination of sprout is given respectively and incubation time is cultivated, when culture to sprout length is 3cm 4cm, stop culture, and collect the cereal of culture.The present invention makes cereal absorb inorganic selenium in immersion process, then pass through illumination cultivation by the way that cereal is soaked in into Selenium-rich nutrient solution, make cereal that the inorganic selenium of absorption is converted into Organic Selenium, territory restriction and the limitation measured, the cost for solving organic selenium-rich are low, make human body intake Se content balanced, and according to《The requirement of DBS42 Hubei Province food security provincial standard organic selenium-rich food Se content》, organic selenium content can reach more than the 80% of total Se content in cereal of the present invention.

Description

A kind of method for making cereal organic selenium-rich using artificial culture
Technical field
The invention belongs to function agricultural technology field, makes cereal organic selenium-rich using artificial culture more particularly to one kind Method.
Background technology
Selenium is a kind of indispensable important trace element of human body, can strengthen the human immunity phase, plays anticancer, regulation dimension Raw plain A, vitamin C, vitamin E, vitamin K absorption and effect, the human body such as utilize internal a certain amount of selenium need to be maintained to contain Amount.
China is Ziyang, shaanxi, enshi, Anhui Shitai County respectively with there was only three natural selenium-rich at present, is caused natural Organic Selenium food production is unable to meet demand at all, and there is also following shortcoming simultaneously for Organic Selenium:First, Organic Selenium Costco Wholesale It is higher, the Organic Selenium of various concentrations, high up to 2,000/kg -1,000,000/kg, it is unfavorable for using on a large scale;Second, city at present Se-enriched yeast on face preferably at most, and yeast belongs to a kind of microbial cells of selenium-rich domestication, is of limited application.And easily Other nutrients are caused to absorb non-uniform phenomenon;3rd:Organic selenium content is unstable, easily causes product quality problem throwing Tell.
At present, selenium-enriched food on the market, nutrition fortifier is added to solve using process mostly, and nutrition is strong Agent belongs to inorganic selenium, and human body also is difficult to absorb.Therefore, it is the skill that needs to be widely popularized at present inorganic selenium to be become Organic Selenium Art.
The content of the invention
It is an object of the invention to provide a kind of method for making cereal organic selenium-rich using artificial culture, can be fully solved In place of above-mentioned the deficiencies in the prior art.
The purpose of the present invention is realized by following technical proposals:
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) Selenium-rich nutrient solution is configured;
2) preparing to need the cereal of organic selenium-rich, be positioned in Selenium-rich nutrient solution and soak 5h-8h, soaking temperature is 15 DEG C- 25℃;
3) by the cereal after immersion, be positioned over temperature be 15 DEG C -25 DEG C, relative humidity be the incubated of 70%-80% 45h-50h is cultivated in room, after checking grain germination " appearing ", opens illumination, maintains intensity of illumination 250lx-350lx, culture 20h-30h, observation cereal sprout are faint yellow, and when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 450lx- 550lx, 45h-55h is cultivated, observation cereal sprout is light green color, and it is 950lx-1050lx to adjust intensity of illumination again, and culture is extremely When sprout length is 2cm-5cm, stop culture, you can harvest the cereal of culture.
Preferably, the soaking temperature of the step 2) Selenium-rich nutrient solution is 18 DEG C -23 DEG C.
Preferably, the soaking temperature of the step 2) Selenium-rich nutrient solution is 20 DEG C.
Preferably, incubated room needs to carry out Aseptic sterilisation processing before culture in the step 3).
Preferably, incubated room temperature is 18 DEG C -23 DEG C in the step 3).
Preferably, incubated room temperature is 20 DEG C in the step 3).
Preferably, the relative humidity in incubated room is 73%-78% in the step 3).
Preferably, in the step 3) during grain germination " appearing ", culture intensity of illumination is 280lx-330lx, culture Time is 22h-26h.
Preferably, cereal sprout is faint yellow in the step 3), when sprout length is 0.5cm-1cm, illumination is cultivated Intensity is 480lx-530lx, incubation time 48h-53h.
Preferably, when cereal sprout is light green color in the step 3), culture intensity of illumination is 980lx-1030lx, Culture to sprout length is 3cm-4cm.
Compared with prior art, the beneficial effects of the present invention are:
1. the present invention makes cereal absorb inorganic selenium in immersion process, then lead to by the way that cereal is soaked in into Selenium-rich nutrient solution Illumination cultivation is crossed, makes cereal that the inorganic selenium of absorption is converted into Organic Selenium, solves the territory restriction of organic selenium-rich and the limit of amount System.
2. cost of the present invention is cheap, easily promote.
3. the present invention is wider than yeast application using cereal, human intake's nutrient will not be caused uneven existing As.
4. Se content is effectively controlled in Organic Selenium of the present invention.
5. basis《The requirement of DBS42 Hubei Province food security provincial standard organic selenium-rich food Se content》, paddy of the present invention Organic selenium content can reach more than the 80% of total Se content in thing.
Embodiment
With reference to specific embodiment, the present invention is further illustrated.
Embodiment one
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) Selenium-rich nutrient solution is configured:The physicochemical property for containing inorganic selenium solution, detecting solution needed is prepared first, according to Physicochemical property, prepare Se-containing nutritive liquid.
2) preparation needs the cereal of organic selenium-rich, the excellent cereal that selected no worm-eaten, nothing are gone mouldy, is installed, placed with mesh bag 5h is soaked in Selenium-rich nutrient solution, soaking temperature is 15 DEG C;
3) take out immersion after cereal, be positioned over the temperature after sterilizing is handled be 15 DEG C, relative humidity be 70% 45h is cultivated in incubated room, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 250lx, cultivate 20h, Cereal sprout is observed to be faint yellow, when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 450lx, cultivates 45h, observation Cereal sprout is " light green color ", again adjust intensity of illumination be 950lx, cultivate to sprout length be 2cm-5cm when, stopping train Support, and harvest the cereal of culture.
According to《The requirement of DBS42 Hubei Province food security provincial standard organic selenium-rich food Se content》, present invention harvest Organic selenium-rich cereal in organic selenium content can reach more than the 80% of total Se content, make organic selenium-rich corn growing by region Limitation and the limitation of amount, cost is cheap, beneficial to popularization.
Embodiment two
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) Selenium-rich nutrient solution is configured:The physicochemical property for containing inorganic selenium solution, detecting solution needed is prepared first, according to Physicochemical property, prepare Se-containing nutritive liquid;
2) preparation needs the cereal of organic selenium-rich, the excellent cereal that selected no worm-eaten, nothing are gone mouldy, is installed, placed with mesh bag 6h is soaked in Selenium-rich nutrient solution, soaking temperature is 18 DEG C;
3) take out immersion after cereal, be positioned over the temperature after sterilizing is handled be 18 DEG C, relative humidity be 73% 48h is cultivated in incubated room, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 280lx, cultivate 22h, Cereal sprout is observed to be faint yellow, when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 480lx, cultivates 48h, observation Cereal sprout is " light green color ", again adjust intensity of illumination be 980lx, cultivate to sprout length be 2cm-5cm when, stopping train Support, and harvest the cereal of culture.
Embodiment three
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) Selenium-rich nutrient solution is configured;The physicochemical property for containing inorganic selenium solution, detecting solution needed is prepared first, according to Physicochemical property, prepare Se-containing nutritive liquid;
2) preparation needs the cereal of organic selenium-rich, the excellent cereal that selected no worm-eaten, nothing are gone mouldy, is installed, placed with mesh bag 7h is soaked in Selenium-rich nutrient solution, soaking temperature is 20 DEG C;
3) take out immersion after cereal, be positioned over the temperature after sterilizing is handled be 20 DEG C, relative humidity be 75% 48h is cultivated in incubated room, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 300lx, cultivate 24h, Cereal sprout is observed to be faint yellow, when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 500lx, cultivates 48h, observation Cereal sprout is " light green color ", again adjust intensity of illumination be 1000lx, cultivate to sprout length be 3cm-4cm when, stopping train Support, and harvest the cereal of culture.
Example IV
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) certain density Selenium-rich nutrient solution is configured:The physics and chemistry for containing inorganic selenium solution, detecting solution needed is prepared first Property, according to physicochemical property, prepare Se-containing nutritive liquid;
2) preparation needs the cereal of organic selenium-rich, the excellent cereal that selected no worm-eaten, nothing are gone mouldy, is installed, placed with mesh bag 7h is soaked in Selenium-rich nutrient solution, soaking temperature is 23 DEG C;
3) take out immersion after cereal, be positioned over the temperature after sterilizing is handled be 23 DEG C, relative humidity be 78% 49h is cultivated in incubated room, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 330lx, cultivate 26h, Cereal sprout is observed to be faint yellow, when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 530lx, cultivates 53h, observation Cereal sprout is " light green color ", again adjust intensity of illumination be 1030lx, cultivate to sprout length be 3cm-4cm when, stopping train Support, and harvest the cereal of culture.
Embodiment five
A kind of method for being made cereal organic selenium-rich using artificial culture, is comprised the following steps:
1) certain density Selenium-rich nutrient solution is configured:The physics and chemistry for containing inorganic selenium solution, detecting solution needed is prepared first Property, according to physicochemical property, prepare Se-containing nutritive liquid;
2) preparation needs the cereal of organic selenium-rich, the excellent cereal that selected no worm-eaten, nothing are gone mouldy, is installed, placed with mesh bag 8h is soaked in Selenium-rich nutrient solution, soaking temperature is 25 DEG C;
3) take out immersion after cereal, be positioned over the temperature after sterilizing is handled be 25 DEG C, relative humidity be 80% 50h is cultivated in incubated room, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 350lx, cultivate 30h, Cereal sprout is observed to be faint yellow, when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 550lx, cultivates 55h, observation Cereal sprout is " light green color ", again adjust intensity of illumination be 1050lx, cultivate to sprout length be 3cm-4cm when, stopping train Support, and harvest the cereal of culture.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.

Claims (10)

  1. A kind of 1. method for making cereal organic selenium-rich using artificial culture, it is characterised in that:Comprise the following steps:
    1) Selenium-rich nutrient solution is configured;
    2) preparation needs the cereal of organic selenium-rich, is positioned in Selenium-rich nutrient solution and soaks 5h-8h, and soaking temperature is 15 DEG C -25 DEG C;
    3) by the cereal after immersion, be positioned over temperature be 15 DEG C -25 DEG C, relative humidity be in 70%-80% incubated room 45h-50h is cultivated, after checking grain germination " appearing ", illumination is opened, maintains intensity of illumination 250lx-350lx, cultivate 20h- 30h, observation cereal sprout are faint yellow, and when sprout length is 0.5cm-1cm, adjustment intensity of illumination is 450lx-550lx, culture 45h-55h, observation cereal sprout be light green color, and adjustment intensity of illumination is 950lx-1050lx again, cultivates to sprout length and is During 2cm-5cm, stop culture, you can harvest the cereal of culture.
  2. A kind of 2. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described The soaking temperature of step 2) Selenium-rich nutrient solution is 18 DEG C -23 DEG C.
  3. A kind of 3. method for making cereal organic selenium-rich using artificial culture according to claim 2, it is characterised in that:It is described The soaking temperature of step 2) Selenium-rich nutrient solution is 20 DEG C.
  4. A kind of 4. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described Incubated room needs to carry out Aseptic sterilisation processing before culture in step 3).
  5. A kind of 5. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described Incubated room temperature is 18 DEG C -23 DEG C in step 3).
  6. A kind of 6. method for making cereal organic selenium-rich using artificial culture according to claim 5, it is characterised in that:It is described Incubated room temperature is 20 DEG C in step 3).
  7. A kind of 7. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described The relative humidity in incubated room is 73%-78% in step 3).
  8. A kind of 8. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described In step 3) during grain germination " appearing ", culture intensity of illumination is 280lx-330lx, incubation time 22h-26h.
  9. A kind of 9. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:It is described Cereal sprout is faint yellow in step 3), and when sprout length is 0.5cm-1cm, culture intensity of illumination is 480lx-530lx, culture Time is 48h-53h.
  10. A kind of 10. method for making cereal organic selenium-rich using artificial culture according to claim 1, it is characterised in that:Institute When stating that cereal sprout is light green color in step 3), culture intensity of illumination is 980lx-1030lx, and it is 3cm- to cultivate to sprout length 4cm。
CN201711103477.2A 2017-11-10 2017-11-10 A kind of method for making cereal organic selenium-rich using artificial culture Pending CN107637446A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103250621A (en) * 2013-06-07 2013-08-21 刘睿 Plant transformation biostate selenium and production method thereof
CN105767904A (en) * 2016-02-22 2016-07-20 傅方胜 Cultivating and processing method of selenium-enriched malt
CN106489398A (en) * 2016-10-28 2017-03-15 广西中科群源农林科技有限公司 A kind of implantation methods of the red polished rice of selenium-rich
CN106962159A (en) * 2017-04-01 2017-07-21 安徽味仙食品有限公司 A kind of breeding method of selenium enrichment cedrela sinensis bud

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103250621A (en) * 2013-06-07 2013-08-21 刘睿 Plant transformation biostate selenium and production method thereof
CN105767904A (en) * 2016-02-22 2016-07-20 傅方胜 Cultivating and processing method of selenium-enriched malt
CN106489398A (en) * 2016-10-28 2017-03-15 广西中科群源农林科技有限公司 A kind of implantation methods of the red polished rice of selenium-rich
CN106962159A (en) * 2017-04-01 2017-07-21 安徽味仙食品有限公司 A kind of breeding method of selenium enrichment cedrela sinensis bud

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