CN107628853B - Special biological fertilizer for corn in growing period and preparation method thereof - Google Patents
Special biological fertilizer for corn in growing period and preparation method thereof Download PDFInfo
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- CN107628853B CN107628853B CN201711105841.9A CN201711105841A CN107628853B CN 107628853 B CN107628853 B CN 107628853B CN 201711105841 A CN201711105841 A CN 201711105841A CN 107628853 B CN107628853 B CN 107628853B
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 56
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 23
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- 238000002360 preparation method Methods 0.000 title description 7
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 20
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000004475 Arginine Substances 0.000 claims abstract description 10
- 239000004471 Glycine Substances 0.000 claims abstract description 10
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- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims abstract description 10
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims abstract description 10
- 239000004472 Lysine Substances 0.000 claims abstract description 10
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 10
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 10
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000004279 alanine Nutrition 0.000 claims abstract description 10
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 10
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000010355 mannitol Nutrition 0.000 claims abstract description 10
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 10
- 239000004474 valine Substances 0.000 claims abstract description 10
- 208000003643 Callosities Diseases 0.000 claims abstract description 7
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 235000009697 arginine Nutrition 0.000 claims description 9
- 235000014304 histidine Nutrition 0.000 claims description 9
- 235000018977 lysine Nutrition 0.000 claims description 9
- 235000014393 valine Nutrition 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 241001614060 Amynthas aspergillus Species 0.000 claims description 3
- 241000243684 Lumbricus Species 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims 2
- 239000002689 soil Substances 0.000 abstract description 11
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 230000000243 photosynthetic effect Effects 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 4
- 238000009825 accumulation Methods 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 235000013877 carbamide Nutrition 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
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- 238000007789 sealing Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000035425 carbon utilization Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Fertilizers (AREA)
Abstract
The special biological fertilizer for the corn in the growing period comprises 52.15-54.01 parts of biological extracting solution, 2.38-2.48 parts of proline, 1.81-1.91 parts of phenylalanine, 5.02-5.20 parts of glycine, 18.07-18.23 parts of histidine, 4.00-4.11 parts of alanine, 4.19-4.32 parts of lysine, 17.44-17.56 parts of valine, 8.60-8.71 parts of arginine, 3.31-3.39 parts of D-mannitol and 850-950 parts of purified water. The special biological fertilizer for the corns in the growing period is added with nutrient substances required by the growth of the corns and the biological extracting solution is added with soil nutrients; the special biological fertilizer for the corn in the growing period has influence on the photosynthetic property of the corn leaf in the growing period, can improve the photosynthetic rate of the corn leaf, and promotes the improvement of the plant height and the stem thickness of the corn and the accumulation of dry matters of the corn.
Description
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a special biological fertilizer for corn in a growing period and a preparation method thereof.
Background
Corn is not only the food and feed king of people, but also an important industrial raw material, and can be processed into more than 3000 industrial products. The corn industry processing in China is rapidly developed, the consumption of corn is greatly increased, and the corn processing method has great influence on the balance of supply and demand and the distribution pattern of corn in China and even the world. The important role of the processing development of the corn industry and the influence thereof are comprehensively known and deeply researched, and scientific development strategies are made, so that the method has very important significance for actively making good the macro regulation of grains, keeping the balance of the corn supply and demand and promoting the economic health development of China. The seed emergence rate is a key factor for the success and failure of corn production. The corn mainly performs root development, leaf growth and stem node differentiation from a four-leaf stage to an elongation stage. The corn seedlings are changed from autotrophy to heterotrophy from the four-leaf stage, the root system gradually starts to absorb soil nutrients, and the leaves strengthen participation in photosynthesis.
Disclosure of Invention
The invention aims to provide a special biological fertilizer for corn in a growing period and a preparation method thereof. The special biological fertilizer for the corn in the growing period can improve the photosynthetic rate of the corn leaves, and promote the plant height and stem thickness of the corn and the dry matter accumulation of the corn.
The special biological fertilizer for the corn in the growing period comprises 52.15-54.01 parts of biological extracting solution, 2.38-2.48 parts of proline, 1.81-1.91 parts of phenylalanine, 5.02-5.20 parts of glycine, 18.07-18.23 parts of histidine, 4.00-4.11 parts of alanine, 4.19-4.32 parts of lysine, 17.44-17.56 parts of valine, 8.60-8.71 parts of arginine, 3.31-3.39 parts of D-mannitol and 850-950 parts of purified water.
The biological extracting solution is prepared by a biological extracting solution extracting device which comprises an extracting tank and an extracting tank bracket arranged at the lower end of the extracting tank; the extraction tank comprises an extraction tank main body and an oxygen injection pipe arranged at the side end of the extraction tank main body; an extract outlet arranged at the lower end of the extraction tank main body; the middle part of the extraction tank main body is transversely provided with a placing net, and two sides of the extraction tank main body are respectively provided with an electrifying socket; the electrified jack is connected with the placing net. The special biological fertilizer for the corns in the growing period is prepared by the following steps:
the method comprises the following steps: selecting 250-350 g of 4-month-old lumbricus teleostomum, washing, and spreading on a placing net of a biological extract extraction device;
step two: introducing air with the oxygen concentration of 48-50% into the oxygen injection pipe, introducing 9-12V voltage into the two electrifying sockets at the same time, keeping for 25 seconds, and collecting the biological extracting solution at an extracting solution outlet;
step three: replacing new Pheretima aspergillum, repeating the above steps, and collecting the required biological extractive solution;
step four: uniformly mixing the biological extract, proline, phenylalanine, glycine, histidine, alanine, lysine, valine, arginine and D-mannitol, and sealing at 35 deg.C for 72 hr;
step five: and (4) mixing and stirring the purified water and the mixture obtained in the fourth step uniformly.
The special biological fertilizer for the corns in the growing period is added with nutrient substances required by the growth of the corns and the biological extracting solution is added with soil nutrients; the special biological fertilizer for the corn in the growing period has influence on the photosynthetic property of the corn leaf in the growing period, can improve the photosynthetic rate of the corn leaf, and promotes the improvement of the plant height and the stem thickness of the corn and the accumulation of dry matters of the corn.
Test materials and methods:
in order to reduce the influence of the difference generated in the corn emergence period on the corn growth period, firstly, the corn 335 corn seeds are cultivated to four leaves, and healthy and uniform seedlings are selected and transplanted into greenhouse soil. The soil to be tested in the greenhouse is northeast black soil containing humus, and the physical characteristics and the nutrient content of the plough layer soil are shown in table 1.
In order to reduce the influence of the uneven soil moisture on the test, the soil in the greenhouse is ploughed before the test is started. The liquid fertilizer application adopts a completely random design.
Seven experiments were performed: examples 1, 2, 3, comparative example 1 of the present invention: the biological extract in the invention; comparative example 2: 2.48 parts of proline, 1.91 parts of phenylalanine, 5.20 parts of glycine, 18.23 parts of histidine, 4.11 parts of alanine, 4.32 parts of lysine, 17.56 parts of valine, 8.71 parts of arginine, 3.39 parts of D-mannitol and 950 parts of purified water are uniformly mixed and stirred; comparative example 3: pure urea (carbamide, formula H2NCONH2 (CO (NH 2) 2)) was diluted with 50 times the weight of water.
When transplanting seedlings, liquid fertilizer is applied to the roots of the corn seedlings at one time, the application amount is 120 g/plant, the application position is within the soil range of 5 cm from the ground surface and the diameter of the roots of the corn is about 10 cm, and then surface soil is covered. The field management in the corn growth test period is watering and weeding, and additional fertilization is not needed. The test indexes are measured 5 days after transplanting, the influence of different experimental groups on the plant height and stem thickness of the corn is measured 55 days later, and the statistical results are shown in table 2.
The nutrient element status of the corn leaves is closely related to the spectral characteristics, and the nutrient status of the corn plants can be reflected by measuring the spectral difference of the corn leaves in the growing period. The SPAD value calculated by the chlorophyll meter is the chlorophyll content of the corn leaf in unit area, and can be used for comparing the nitrogen content of the corn leaf in unit area. The SPAD value of the leaf in the growing period of the corn seedling under the treatment of different experimental groups is recorded in the experiment and is shown in a table 3.
It can be seen from table 2 that example 1/2/3 and comparative example 1/2/3 both have significant promoting effects on plant height and stem thickness of corn compared to the control; the two indexes of the embodiment 1/2/3 have more obvious promoting effect; as can be seen from Table 3, the SPAD value of the leaves gradually increased during the growing period of the corn as measured by the test; the SPAD values of the fertilized treated maize leaves peaked 55 days after transplantation and were clearly greater for example 1/2/3 than for comparative example 1/2/3.
Drawings
FIG. 1 is a schematic view of the structure of the device for extracting biological extract according to the present invention.
Detailed Description
The following examples are intended to further illustrate the present invention and should not be construed as limiting the scope of the invention, and other insubstantial modifications and adaptations of the invention by those skilled in the art based on the teachings herein are intended to be covered thereby.
Example 1: the special biological fertilizer for the corn in the growing period is prepared from the following raw materials in parts by weight: 53.66 g of biological extract, 2.42 g of proline, 1.86 g of phenylalanine, 5.11 g of glycine, 18.15 g of histidine, 4.05 g of alanine, 4.26 g of lysine, 17.51 g of valine, 8.66 g of arginine, 3.36 g of D-mannitol and 900 g of purified water.
The biological extracting solution is prepared by a biological extracting solution extracting device which comprises an extracting tank and an extracting tank bracket 1 arranged at the lower end of the extracting tank; the extraction tank comprises a polyvinyl chloride extraction tank main body 2 with the diameter of 15 cm and an oxygen injection pipe 3 arranged at the side end of the extraction tank main body 2; an extract liquid outlet 4 arranged at the lower end of the extraction tank main body 2; the diameter ratio of the oxygen injection pipe 3 to the liquid taking outlet 4 is 3: 1; the middle part of the extraction tank body 2 is transversely provided with a placing net 5 made of a 20-mesh conductive copper net of the constant water Bodun filtration science and technology Limited company, and two sides of the extraction tank body 2 are respectively provided with an electrifying socket 6; the power-on socket 6 is connected with the placing net 5.
The preparation method of the special biological fertilizer for the corns in the growing period comprises the following steps:
the method comprises the following steps: selecting 250-350 g of 4-month-old lumbricus teleostomum, washing, and spreading on a placing net of a biological extract extraction device;
step two: introducing air with the oxygen concentration of 48-50% into the oxygen injection pipe, introducing 9-12V voltage into the two electrifying sockets, keeping for 25 seconds, and collecting biological extracting solution at an extracting solution outlet, wherein the extraction rate is 19.01%;
step three: replacing new Pheretima aspergillum, repeating the above steps, and collecting the required biological extractive solution;
step four: uniformly mixing 53.66 g of biological extract, 2.42 g of proline, 1.86 g of phenylalanine, 5.11 g of glycine, 18.15 g of histidine, 4.05 g of alanine, 4.26 g of lysine, 17.51 g of valine, 8.66 g of arginine and 3.36 g of D-mannitol, and sealing and keeping for 72 hours at the temperature of 35 ℃;
step five: 950 g of purified water and the mixture obtained in the fourth step are mixed and stirred uniformly.
Example 2: the preparation method of the special biological fertilizer for the corn in the growing period is the same as that in the example 1, and the special biological fertilizer is prepared from the following raw materials in parts by weight: 52.15 g of biological extract, 2.38 g of proline, 1.81 g of phenylalanine, 5.02 g of glycine, 18.07 g of histidine, 4.00 g of alanine, 4.19 g of lysine, 17.44 g of valine, 8.60 g of arginine, 3.31 g of D-mannitol and 850 g of purified water.
Example 3: the preparation method of the special biological fertilizer for the corn in the growing period is the same as that in the example 1, and the special biological fertilizer is prepared from the following raw materials in parts by weight:
54.01 g of biological extract, 2.48 g of proline, 1.91 g of phenylalanine, 5.20 g of glycine, 18.23 g of histidine, 4.11 g of alanine, 4.32 g of lysine, 17.56 g of valine, 8.71 g of arginine, 3.39 g of D-mannitol and 950 g of purified water.
Claims (1)
1. The special biological fertilizer for the corn in the growing period is characterized by being prepared from the following raw materials in parts by weight: 52.15-54.01 parts of biological extracting solution, 2.38-2.48 parts of proline, 1.81-1.91 parts of phenylalanine, 5.02-5.20 parts of glycine, 18.07-18.23 parts of histidine, 4.00-4.11 parts of alanine, 4.19-4.32 parts of lysine, 17.44-17.56 parts of valine, 8.60-8.71 parts of arginine, 3.31-3.39 parts of D-mannitol and 850-950 parts of purified water;
the biological extracting solution is prepared by a biological extracting solution extracting device, and the biological extracting solution extracting device comprises an extracting tank and an extracting tank support arranged at the lower end of the extracting tank; the extraction tank comprises an extraction tank main body and an oxygen injection pipe arranged at the side end of the extraction tank main body; an extract liquid outlet arranged at the lower end of the extraction tank main body; the middle part of the extraction tank main body is transversely provided with a placing net, and two sides of the extraction tank main body are respectively provided with an electrifying socket; the power-on socket is connected with the placing net;
the special biological fertilizer for the corns in the growing period is prepared by the following steps:
the method comprises the following steps: selecting 250-350 g of 4-month-old lumbricus teleostomum, washing, and spreading on a placing net of a biological extract extraction device;
step two: introducing air with the oxygen concentration of 48-50% into the oxygen injection pipe, introducing 9-12V voltage into the two electrifying sockets at the same time, keeping for 25 seconds, and collecting the biological extracting solution at an extracting solution outlet;
step three: replacing new Pheretima aspergillum, repeating the above steps, and collecting the required biological extractive solution;
step four: uniformly mixing the biological extracting solution, proline, phenylalanine, glycine, histidine, alanine, lysine, valine, arginine and D-mannitol in parts by weight, and keeping the mixture in a sealed state for 72 hours at the temperature of 35 ℃;
step five: and (3) mixing and stirring the purified water and the mixture obtained in the fourth step uniformly.
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| CN104803779A (en) * | 2015-04-22 | 2015-07-29 | 天津贾立明蚯蚓养殖有限公司 | Amino acid fertilizer prepared from lumbricus amino acid and fulvic acid potassium by chelate method and preparation method of amino acid fertilizer |
| AU2015101544A4 (en) * | 2015-10-18 | 2015-11-19 | Udagepolage Don, Manjula Nishantha MR | Dual Chelation of Soluble or Liquid Fertilizer and Minerals with Amino Acid and Complex Polymeric Polyhydroxy Acids for Agricultural Use |
| CN107021824A (en) * | 2017-05-02 | 2017-08-08 | 罗格平 | A kind of full nutrient formulation liquid fertilizer based on sharp gas working system |
| CN107285962A (en) * | 2017-08-02 | 2017-10-24 | 天津天丰泽田生物科技有限公司 | A kind of Water soluble fertilizer containing earth-worm extractive as raw material |
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