CN107624475A - A kind of method for culturing seedlings of the fruit of a cubeb litsea tree - Google Patents
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree Download PDFInfo
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- CN107624475A CN107624475A CN201710890456.3A CN201710890456A CN107624475A CN 107624475 A CN107624475 A CN 107624475A CN 201710890456 A CN201710890456 A CN 201710890456A CN 107624475 A CN107624475 A CN 107624475A
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- fruit
- cubeb litsea
- litsea tree
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to seedling-raising technique field, more particularly to a kind of method for culturing seedlings of the fruit of a cubeb litsea tree；Comprise the following steps：(1) planting site processing, (2) Seeds preprocess, (3) vernalization processing, (4) sowing, (5) water and fertilizer management；The method that the present invention takes seeding and seedling raising, it is effectively promoted the differentiation of seed coat cell growth, shorten growing-seedling period, improve the resistance of fruit of a cubeb litsea tree seedling and the adaptive faculty to environment, improve survival rate, using the breeding method of the present invention, for the survival rate of fruit of a cubeb litsea tree seedling up to more than 93%, the incidence of disease of fruit of a cubeb litsea tree seedling is effectively down to less than 3.5%.
Description
Technical field
The present invention relates to seedling-raising technique field, more particularly to a kind of method for culturing seedlings of the fruit of a cubeb litsea tree.
Background technology
The fruit of a cubeb litsea tree, also known as cubeb litsen tree, Tetranthera citrata, Alpinia japonica, Litsea chenii, Litsea pungens, Lauraceae, Litsea machaka or
Dungarunga, up to 8-10 rice, treelet bark yellow green is smooth, veteran bark taupe, and the derived essential oil in the fruit of a cubeb litsea tree mainly contains
In fruit, containing essential oil typically 3~4% in pericarp, wherein fixed main chemical compositions have as many as 17 kinds, its property,
Purposes is different, and principal component citral is up to 80%, and the fruit of a cubeb litsea tree has the effect of warming spleen and stomach for dispelling cold, regulating qi-flowing for relieving pain, available for stomach cold
The diseases such as caused hiccup vomiting, epigastric pain, it can also be used to caused by cold hernia stomachache, sympotoms caused by cold factors difficult urination and children's stagnancy of cold-damp
Turbid urine, it can also treat the diseases such as anemofrigid cold, cough and asthma, indigestion.External application is mashed with fruit of a cubeb litsea tree leaf, subcutaneous ulcer furuncle can be treated
Swelling and pain；The pan-fried hydro-thermal of litsea cubeba root is applied or hot dipping can treat treating rheumatic ostealgia, numb limb, waist-leg disease and traumatic injury.Litsea citrate oil
The raw material of synthesising complex E, K, A etc. is alternatively arranged as, to play its effect in medicine.Because litsea citrate oil is in treatment disease
In unique effect, in terms of clinical research and focus.In addition, the fruit of a cubeb litsea tree also have relieving asthma, antiallergy, the anti-rhythm of the heart often,
Antithrombotic, antibacterial, antiviral, anti-Trichomonas vaginalis effect.It is contemplated that application of the litsea citrate oil in terms of medicine will also obtain into
Exhibition.
In view of the abundant edible and medical value of the fruit of a cubeb litsea tree, its application is very extensive, and its market demand is very big,
The state that supply falls short of demand is lain substantially in, the fruit of a cubeb litsea tree has cultivation in China various regions at present, and scale constantly expands, and cultivates mountain
Grey son, the cultivation of fruit of a cubeb litsea tree seedling is crucial, and in the prior art, the investigative technique on fruit of a cubeb litsea tree vegetative propagation is various, and by
It is incorrect in the nursery processing mode at present for the fruit of a cubeb litsea tree, cause that fruit of a cubeb litsea tree resistance is poor, and incidence of insect disease is higher, sternly
The survival rate of the fruit of a cubeb litsea tree is reduced again, in consideration of it, how to be directed to the growth characteristics of the fruit of a cubeb litsea tree and current seedling-raising technique is changed
Enter, fruit of a cubeb litsea tree resistance is improved by the nursery of reasonable science, the risk of pest and disease damage infringement is reduced, improves surviving for the fruit of a cubeb litsea tree
Rate, to meet that the needs of existing market becomes particularly important.
The content of the invention
The present invention is in order to solve the above technical problems, provide a kind of method for culturing seedlings of the fruit of a cubeb litsea tree.
Realized particular by following technical scheme：
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 50~60cm,
Spraying function liquid during ploughing deeply, place one week naturally；
Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree 12~18, stone-like omphalia 6~8, frutus cnidii 3
~5, Fructus Corni 6~8, the flower bud of lily magnolia 2~4, duckweed 3~6, coffee grounds 15~25, wormcast 8~12, edible fungi residue 12~18,
Clothing bacillus 3~5, Paecilomyces lilacinus 4~6, bacillus laterosporus 3~5；
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；
(3) vernalization is handled：By step (2) processing after seed be laid in germinating cabinet, on the surface of the seed cover 0.5~
0.8cm fine earth, spray germination liquid on fine earth surface so that soil moisture in layer maintains 30~40%, until germination；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 80~120kg.
Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree 15, stone-like omphalia 7, frutus cnidii 4, Fructus Corni
7th, the flower bud of lily magnolia 3, duckweed 5, coffee grounds 20, wormcast 10, edible fungi residue 15, bacillus licheniformis 4, Paecilomyces lilacinus 5, side spore bud
Spore bacillus 4.
Described functional liquid, its preparation method comprise the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, 60~70min of infusion, the ethanol of 10 times of amounts of the crushed material quality are added, 60
15~25min is ultrasonically treated under~70KHz frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 38~42 DEG C, relative humidity is 80~90%
In the environment of ferment 4~5d, drying and crushing, be designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
Described thimerosal, it is by 0.8~1.2 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.4~0.6：
0.8~1.2：0.3~0.5 mass ratio mixing, adds water 30~40min of infusion, filters, take filtrate.
Described germinating cabinet, temperature are 33~38 DEG C, and relative humidity is 70~80%.
Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.1~0.3:
0.2~0.4:0.3~0.5:0.8~1.2 mass ratio mixing, tempers into the solution that content is 30~40%.
Described nutrient solution, it is made up of following raw material by weight：Ammonium nitrate 15~25, potassium sulfate 8~12, cross phosphorus
Sour calcium 8~20, zinc sulfate 2~4, sodium selenate 1~3, ferrous sulfate 0.8~1.2, boric acid 0.6~0.8, glycine 3~5, color ammonia
Acid 2~4, arginine 6~8, biotin 5~8, vitamin B13~8.
Described nutrient solution, it is by ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Mixing, the water of 1.2~1.6 times of amounts of mixture is added, is dissolved by heating, add glycine, tryptophan, arginine, dimension life
Plain H, vitamin B1Mix, load watering can.
In summary, the beneficial effects of the present invention are：The method that the present invention takes seeding and seedling raising, is effectively promoted seed
Epidermal growth breaks up, and shortens growing-seedling period, improves the resistance of fruit of a cubeb litsea tree seedling and the adaptive faculty to environment, raising is deposited
Motility rate, using the breeding method of the present invention, up to more than 93%, the incidence of disease of fruit of a cubeb litsea tree seedling has the survival rate of fruit of a cubeb litsea tree seedling
Effect is down to less than 3.5%.
By processing of the functional liquid to soil, worm's ovum and the miscellaneous bacteria of soil can be killed, is carried for the development of fruit of a cubeb litsea tree seed
For a security context, soil beneficial bacterium is also added in functional liquid, strengthens buffering power of soil, water conservation moisturizing, strengthens mountain
Grey sub- drought resisting, cold-resistant, waterlogging-resistant ability；After being manured into soil, rapid breeding turns into dominant microflora, controls rhizosphere nutrition and resource, makes
The pathogen such as continuous cropping, root-rot, vertical withered, gummosis, grey mold loses living space and condition.Increase the cell membrane of root system of plant cell
Thickness, fibrosis, lignifying, and the double silicon layers of cutin are generated, the firm barrier for preventing pathogen from attacking is formed, meanwhile, bacterial metabolism
Caused plant endogenous enzyme and plant growth regulator enter in plant via root system, can promote fruit of a cubeb litsea tree leaf photosynthesis
Enhancing, after the fermentation process of organic matter, effectively enhance the fertilizer of soil, the nutrition needed for growth provided for the fruit of a cubeb litsea tree.
By the immersion of thimerosal, be advantageous to kill the worm's ovum for being attached to the surface of the seed, lift the quality of seed, be the later stage
The growth situation of the fruit of a cubeb litsea tree provides basic guarantee, carries out vernalization processing to seed in certain circumstances, by the processing of germination liquid,
Promote the differentiation and growth of seed coat tissue, promote rudiment, shorten growth cycle, after planting make fruit of a cubeb litsea tree root growth more
Prosperity ensures the quality of growth.
Embodiment
The embodiment of the present invention is described in further detail below, but the invention is not limited in these realities
Mode is applied, any improvement or replacement on the present embodiment essence spirit, it is claimed to still fall within the claims in the present invention
Scope.
Embodiment 1
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 50cm, plough deeply
During spraying function liquid, naturally place one week；Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree
12kg, stone-like omphalia 6kg, frutus cnidii 3kg, Fructus Corni 6kg, flower bud of lily magnolia 2kg, duckweed 3kg, coffee grounds 15kg, wormcast 8kg, edible mushroom
Bacteria residue 12kg, bacillus licheniformis 3kg, Paecilomyces lilacinus 4kg, bacillus laterosporus 3kg；Described functional liquid, its preparation side
Method comprises the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, infusion 60min, the ethanol of 10 times of amounts of the crushed material quality are added, 60KHz's
15min is ultrasonically treated under frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 38 DEG C, in the environment of relative humidity is 80%
Ferment 4~5d, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；Described thimerosal, it is by 0.8 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.4：
0.8：0.3 mass ratio mixing, adds water infusion 30min, filters, take filtrate；
(3) vernalization is handled：Seed after step (2) processing is laid in 33 DEG C, relative humidity is in 70% germinating cabinet,
0.5~0.8cm fine earth is covered on the surface of the seed, sprays germination liquid on fine earth surface so that soil moisture in layer maintains
30%, until germination；Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.1:
0.2:0.3:0.8 mass ratio mixing, tempers into the solution that content is 30%；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 80kg；Described nutrient solution,
It is made up of following raw material by weight：Ammonium nitrate 15kg, potassium sulfate 8kg, calcium superphosphate 8kg, zinc sulfate 2kg, sodium selenate
1kg, ferrous sulfate 0.8kg, boric acid 0.6kg, glycine 3kg, tryptophan 2kg, arginine 6kg, biotin 5kg, vitamin
B13kg；Described nutrient solution, it is to mix ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Close, add the water of 1.2~1.6 times of amounts of mixture, dissolve by heating, add glycine, tryptophan, arginine, vitamin
H, vitamin B1Mix, load watering can.
Embodiment 2
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 60cm, plough deeply
During spraying function liquid, naturally place one week；Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree
18kg, stone-like omphalia 8kg, frutus cnidii 5kg, Fructus Corni 8kg, flower bud of lily magnolia 4kg, duckweed 6kg, coffee grounds 25kg, wormcast 12kg, edible mushroom
Bacteria residue 18kg, bacillus licheniformis 5kg, Paecilomyces lilacinus 6kg, bacillus laterosporus 5kg；Described functional liquid, its preparation side
Method comprises the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, infusion 70min, the ethanol of 10 times of amounts of the crushed material quality are added, 70KHz's
25min is ultrasonically treated under frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 42 DEG C, in the environment of relative humidity is 90%
Ferment 4~5d, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；Described thimerosal, it is by 1.2 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.6：
1.2：0.5 mass ratio mixing, adds water infusion 40min, filters, take filtrate；
(3) vernalization is handled：Seed after step (2) processing is laid in 38 DEG C, relative humidity is in 80% germinating cabinet,
0.5~0.8cm fine earth is covered on the surface of the seed, sprays germination liquid on fine earth surface so that soil moisture in layer maintains
40%, until germination；Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.3:
0.4:0.5:1.2 mass ratio mixing, tempers into the solution that content is 40%；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 120kg；Described nutrient solution,
It is made up of following raw material by weight：Ammonium nitrate 25kg, potassium sulfate 12kg, calcium superphosphate 20kg, zinc sulfate 4kg, selenic acid
Sodium 3kg, ferrous sulfate 1.2kg, boric acid 0.8kg, glycine 5kg, tryptophan 4kg, arginine 8kg, biotin 8kg, vitamin
B18kg；Described nutrient solution, it is to mix ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Close, add the water of 1.2~1.6 times of amounts of mixture, dissolve by heating, add glycine, tryptophan, arginine, vitamin
H, vitamin B1Mix, load watering can.
Embodiment 3
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 50~60cm,
Spraying function liquid during ploughing deeply, place one week naturally；Described functional liquid, it is made up of following raw material by weight：Mountain
Grey sub- 15kg, stone-like omphalia 7kg, frutus cnidii 4kg, Fructus Corni 7kg, flower bud of lily magnolia 3kg, duckweed 5kg, coffee grounds 20kg, wormcast 10kg, food
With bacterium bacteria residue 15kg, bacillus licheniformis 4kg, Paecilomyces lilacinus 5kg, bacillus laterosporus 4kg；Described functional liquid, it is made
Preparation Method comprises the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, infusion 65min, the ethanol of 10 times of amounts of the crushed material quality are added, 65KHz's
20min is ultrasonically treated under frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 40 DEG C, in the environment of relative humidity is 85%
Ferment 4~5d, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；Described thimerosal, it is by 1 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.5：1：
0.4 mass ratio mixing, adds water 30~40min of infusion, filters, take filtrate；
(3) vernalization is handled：Seed after step (2) processing is laid in 35 DEG C, relative humidity is in 75% germinating cabinet,
0.5~0.8cm fine earth is covered on the surface of the seed, sprays germination liquid on fine earth surface so that soil moisture in layer maintains
35%, until germination；Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.2:
0.3:0.4:1 mass ratio mixing, tempers into the solution that content is 35%；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 100kg；Described nutrient solution,
It is made up of following raw material by weight：Ammonium nitrate 20kg, potassium sulfate 10kg, calcium superphosphate 14kg, zinc sulfate 3kg, selenic acid
Sodium 2kg, ferrous sulfate 1kg, boric acid 0.7kg, glycine 4kg, tryptophan 3kg, arginine 7kg, biotin 7kg, vitamin
B15kg；Described nutrient solution, it is to mix ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Close, add the water of 1.2~1.6 times of amounts of mixture, dissolve by heating, add glycine, tryptophan, arginine, vitamin
H, vitamin B1Mix, load watering can.
Embodiment 4
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 50~60cm,
Spraying function liquid during ploughing deeply, place one week naturally；Described functional liquid, it is made up of following raw material by weight：Mountain
Grey sub- 12kg, stone-like omphalia 8kg, frutus cnidii 3kg, Fructus Corni 8kg, flower bud of lily magnolia 2kg, duckweed 6kg, coffee grounds 15kg, wormcast 12kg, food
With bacterium bacteria residue 12kg, bacillus licheniformis 5kg, Paecilomyces lilacinus 4kg, bacillus laterosporus 5kg；Described functional liquid, it is made
Preparation Method comprises the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, infusion 70min, the ethanol of 10 times of amounts of the crushed material quality are added, 60KHz's
25min is ultrasonically treated under frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 38 DEG C, in the environment of relative humidity is 90%
Ferment 4~5d, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；Described thimerosal, it is by 0.8 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.6：
0.8：0.5 mass ratio mixing, adds water infusion 30min, filters, take filtrate；
(3) vernalization is handled：Seed after step (2) processing is laid in 33 DEG C, relative humidity is in 80% germinating cabinet,
0.5~0.8cm fine earth is covered on the surface of the seed, sprays germination liquid on fine earth surface so that soil moisture in layer maintains
30%, until germination；Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.1:
0.4:0.3:1.2 mass ratio mixing, tempers into the solution that content is 40%；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 80kg；Described nutrient solution,
It is made up of following raw material by weight：Ammonium nitrate 15kg, potassium sulfate 12kg, calcium superphosphate 8kg, zinc sulfate 4kg, sodium selenate
1kg, ferrous sulfate 1.2kg, boric acid 0.6kg, glycine 5kg, tryptophan 2kg, arginine 8kg, biotin 5kg, vitamin
B18kg；Described nutrient solution, it is to mix ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Close, add the water of 1.2~1.6 times of amounts of mixture, dissolve by heating, add glycine, tryptophan, arginine, vitamin
H, vitamin B1Mix, load watering can.
Embodiment 5
A kind of method for culturing seedlings of the fruit of a cubeb litsea tree, comprises the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 60cm, plough deeply
During spraying function liquid, naturally place one week；Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree
18kg, stone-like omphalia 6kg, frutus cnidii 5kg, Fructus Corni 8kg, flower bud of lily magnolia 2kg, duckweed 6kg, coffee grounds 15kg, wormcast 12kg, edible mushroom
Bacteria residue 12kg, bacillus licheniformis 5kg, Paecilomyces lilacinus 4kg, bacillus laterosporus 5kg；Described functional liquid, its preparation side
Method comprises the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, described in addition
The water of 1~2 times of crushed material quality amount, infusion 70min, the ethanol of 10 times of amounts of the crushed material quality are added, 70KHz's
25min is ultrasonically treated under frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 38 DEG C, in the environment of relative humidity is 90%
Ferment 4~5d, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
(2) Seeds preprocess：Choose full grains, the fruit of a cubeb litsea tree seed that has no mechanical damage, be put into immersion 3 in thimerosal~
4h, pull out and drain；Described thimerosal, it is by 1.2 by Cephalotaxus mannii, fructus viticis, Chinese knotweed herb and Chinese wampee leaf：0.4：
1.2：0.3 mass ratio mixing, adds water infusion 40min, filters, take filtrate；
(3) vernalization is handled：Seed after step (2) processing is laid in 38 DEG C, relative humidity is in 70% germinating cabinet,
0.5~0.8cm fine earth is covered on the surface of the seed, sprays germination liquid on fine earth surface so that soil moisture in layer maintains
40%, until germination；Described germination liquid, be by concentration be 40% potassium nitrate, leucine, tryptophan and serine by 0.3:
0.2:0.5:0.8 mass ratio mixing, tempers into the solution that content is 40%；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 120kg；Described nutrient solution,
It is made up of following raw material by weight：Ammonium nitrate 25kg, potassium sulfate 8kg, calcium superphosphate 20kg, zinc sulfate 2kg, sodium selenate
3kg, ferrous sulfate 0.8kg, boric acid 0.8kg, glycine 3kg, tryptophan 4kg, arginine 6kg, biotin 8kg, vitamin
B13kg；Described nutrient solution, it is to mix ammonium nitrate, potassium sulfate, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid
Close, add the water of 1.2~1.6 times of amounts of mixture, dissolve by heating, add glycine, tryptophan, arginine, vitamin
H, vitamin B1Mix, load watering can.
The invention will be further described below：
1st, the nursery of the fruit of a cubeb litsea tree is carried out with the method for culturing seedlings of the present embodiment 1~5 respectively, the nursery situation to the fruit of a cubeb litsea tree is carried out
Statistics, as a result as shown in table 1：
Table 1
| Comparative run | Survival rate (%) | Incidence of disease % |
| Embodiment 1 | 93.2 | 3.5 |
| Embodiment 2 | 93.5 | 3.4 |
| Embodiment 3 | 93.8 | 3.1 |
| Embodiment 4 | 93.4 | 3.3 |
| Embodiment 5 | 93.3 | 3.2 |
Claims (8)
1. a kind of method for culturing seedlings of the fruit of a cubeb litsea tree, it is characterised in that comprise the following steps：
(1) planting site is handled：Selection fertile soil, soil layer are abundant, the convenient sand mold soil of draining, plough deeply 50~60cm, plough deeply
During spraying function liquid, naturally place one week；
Described functional liquid, it is made up of following raw material by weight：The fruit of a cubeb litsea tree 12~18, stone-like omphalia 6~8, frutus cnidii 3~5,
Fructus Corni 6~8, the flower bud of lily magnolia 2~4, duckweed 3~6, coffee grounds 15~25, wormcast 8~12, edible fungi residue 12~18, lichens bud
Spore bacillus 3~5, Paecilomyces lilacinus 4~6, bacillus laterosporus 3~5；
(2) Seeds preprocess：The fruit of a cubeb litsea tree seed for choose full grains, having no mechanical damage, 3~4h of immersion in thimerosal is put into,
Pull out and drain；
(3) vernalization is handled：Seed after step (2) processing is laid in germinating cabinet, 0.5~0.8cm is covered on the surface of the seed
Fine earth, fine earth surface spray germination liquid so that soil moisture in layer maintains 30~40%, until germination；
(4) sow；
(5) water and fertilizer management：After seedling is broken ground, to seedling spraying nutrient solution, the mu amount of spraying is 80~120kg.
2. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1, it is characterised in that described functional liquid, by it is following by weight
The raw material composition of part meter：The fruit of a cubeb litsea tree 15, stone-like omphalia 7, frutus cnidii 4, Fructus Corni 7, the flower bud of lily magnolia 3, duckweed 5, coffee grounds 20, wormcast 10,
Edible fungi residue 15, bacillus licheniformis 4, Paecilomyces lilacinus 5, bacillus laterosporus 4.
3. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1 or 2, it is characterised in that described functional liquid, its preparation method
Comprise the following steps：
A, the fruit of a cubeb litsea tree, stone-like omphalia, frutus cnidii, Fructus Corni, the flower bud of lily magnolia and duckweed are weighed by formula, drying and crushing, adds the crushing
Expect the water of 1~2 times of quality amount, 60~70min of infusion, add the ethanol of 10 times of amounts of the crushed material quality, 60~
15~25min is ultrasonically treated under 70KHz frequency, reclaims ethanol, residue is collected, is designated as material 1；
B, coffee grounds, wormcast and edible fungi residue are weighed by formula, at 38~42 DEG C, relative humidity is 80~90% ring
Ferment 4~5d under border, drying and crushing, is designated as material 2；
C, material 1, material 2, bacillus licheniformis, Paecilomyces lilacinus and bacillus laterosporus are mixed, mixed thoroughly.
4. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1, it is characterised in that described thimerosal, be by Cephalotaxus mannii,
Fructus viticis, Chinese knotweed herb and Chinese wampee leaf press 0.8~1.2：0.4~0.6：0.8~1.2：0.3~0.5 mass ratio mixing, adds water
30~40min of infusion, filtering, takes filtrate.
5. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1, it is characterised in that described germinating cabinet, temperature are 33~38
DEG C, relative humidity is 70~80%.
6. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1, it is characterised in that described germination liquid, be to be by concentration
40% potassium nitrate, leucine, tryptophan and serine presses 0.1~0.3:0.2~0.4:0.3~0.5:0.8~1.2 quality
Than mixing, the solution that content is 30~40% is tempered into.
7. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 1, it is characterised in that described nutrient solution, by it is following by weight
The raw material composition of part meter：Ammonium nitrate 15~25, potassium sulfate 8~12, calcium superphosphate 8~20, zinc sulfate 2~4, sodium selenate 1~3,
Ferrous sulfate 0.8~1.2, boric acid 0.6~0.8, glycine 3~5, tryptophan 2~4, arginine 6~8, biotin 5~8, dimension
Raw plain B13~8.
8. the method for culturing seedlings of the fruit of a cubeb litsea tree as claimed in claim 7, it is characterised in that described nutrient solution, be by ammonium nitrate, sulphur
Sour potassium, calcium superphosphate, zinc sulfate, sodium selenate, ferrous sulfate and boric acid mixing, add described 1.2~1.6 times of amounts of mixture
Water, dissolve by heating, add glycine, tryptophan, arginine, biotin, vitamin B1Mix, load watering can.
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| CN107690870A (en) * | 2017-09-27 | 2018-02-16 | 贵州蓝天生态农业科技发展有限公司 | A kind of method for culturing seedlings of Momordica grosvenori |
| CN108401764A (en) * | 2018-03-02 | 2018-08-17 | 合肥浦邦农业科技有限公司 | A kind of high-yield planting method of the fruit of a cubeb litsea tree |
| CN111955299A (en) * | 2020-08-31 | 2020-11-20 | 丽水市林业技术推广总站 | High-germination-rate drought-resistant polygonatum cyrtonema planting method |
| CN112205228A (en) * | 2020-09-16 | 2021-01-12 | 桂林市林业和园林综合服务中心 | Cultivation method for artificially promoting natural renewal of litsea cubeba |
| CN114451095A (en) * | 2022-02-21 | 2022-05-10 | 重庆市林业科学研究院 | Method for improving germination rate of cinnamomum pantherinum seeds |
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