CN107619336A - A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation - Google Patents
A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation Download PDFInfo
- Publication number
- CN107619336A CN107619336A CN201710194858.XA CN201710194858A CN107619336A CN 107619336 A CN107619336 A CN 107619336A CN 201710194858 A CN201710194858 A CN 201710194858A CN 107619336 A CN107619336 A CN 107619336A
- Authority
- CN
- China
- Prior art keywords
- marine alga
- fermentation
- sea
- tangle
- bacterium solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation, using marine alga as raw material, after over cleaning, chopping, marine alga is fermented using bacillus subtilis as fermentation strain, obtain the marine alga liquid containing bacillus subtilis, using this zymotic fluid as base fluid, agriculture field can be applied to as agricultural fertilizer.Extraction process includes the pretreatment of sea-tangle, the preparation of bacillus subtilis seed liquor, the several steps of fermentation of the fermentation and marine alga of bacillus subtilis.The present invention acts on sea-tangle using microorganism caused compound enzyme system and a variety of metabolites in growth course, temperature is improved in sea-tangle fermentation process, played a role for enzyme system caused by microorganism and optimum condition is provided so that the recovery rate and viscosity of algal polysaccharides are greatly improved.Heating contributes to Bacillus subtillis to form gemma simultaneously, improves the survival rate of thalline.
Description
Technical field
The present invention relates to a kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation, belong to agricultural fertilizer
Technology manufacture field.
Background technology
Potato is nutritious, instant edible Main Foods, it has also become in the world after rice, wheat, corn
The fourth-largest cereal crops.The sustainable and stable development of China potato production in recent years, it has also become the of potato production and consumption
One big country.It is relatively low in the tradition plantation yield of potato, influenceed plus by pest and disease damage, under the potato wedge quality for causing potato
Drop, deteriorate soil environment largely using agricultural chemicals, chemical fertilizer in the plantation of potato in addition, huge pressure is brought to ecological environment
Power.Exploring the new method of potato planting turns into very important problem.
Marine alga bacterium solution refers to act on sea-tangle using using enzyme system caused by microorganism, carry out sea-tangle cell broken wall and
The conversion of macromolecular, extraction process is gentle, and the active material in marine alga can be stayed in marine alga liquid, fermentation strain and its metabolism production
Thing can be also remained in marine alga bacterium solution, then is applied to after adding a small amount of low analysis fertilizer composition in agricultural production.Using prebiotic
Antagonism between bacterium and pathogen, being capable of effective disease and pest control.Plant growth-promoting effect hormone can be produced simultaneously, to plant
Root growth, fruit maturation, have a significant effect.Containing abundant nutriment in sea-tangle, for example, starch, vitamin, more
Sugar, mannitol, unrighted acid etc., it is the good raw material for manufacturing natural organic fertilizer.Polysaccharose substance contained therein is
Natural growth regulator, the photosynthesis of plant is may advantageously facilitate, adjust the balance of endogenous hormones, be advantageous to fruit, rhizome
Growth.Contained uronic acid glycoside material, can improve soil texture, improve the water holding capacity of soil in marine alga, promote
The growth of plant rhizosphere probiotics.Compared with traditional chemical fertilizer, alga fertilizer is promoting plant growth, improves crop yield, and
Obvious advantage is suffered from terms of green non-pollution.
The technology remains in theoretical research stage at present, low mainly due to marine alga degradation efficiency, and polyoses content is not up to
To national standard, Strain survival rate is low and production cost is higher.To solve this series of problems, the present invention is with microbial fermentation
For core, a kind of marine alga degradation efficiency height is developed, marine alga active material enriches, probiotics survival efficiency high, lower-cost
New technology.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of special using microbial fermentation preparation potato
With the method for marine alga bacterium solution, the recovery rate and viscosity of algal polysaccharides are improved, while improves the survival rate of bacterial strain, and effectively reduces life
Produce cost.
To achieve the above object, the present invention uses following technical scheme：
1. a kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation, it is characterized in that, including following technique step
Suddenly：
（1）Marine alga pre-processes：Marine alga is soaked to, cleaned up the silt on surface, is shredded with vegetable-chopper, then sea-tangle Pasteur is gone out
Bacterium, cooling are standby.
（2）It is prepared by seed liquor：Bacillus subtilis is activated on activation flat board, the bacillus subtilis on picking flat board
Single bacterium colony, it is inoculated in seed culture medium and is incubated overnight.
（3）Probiotics fermention：Seed culture medium access liquid fermentation tank is fermented.
（4）Marine alga throws tank：Sea-tangle through pasteurization is added in fermentation tank, improves fermentation temperature, other conditions are constant,
Produce marine alga bacterium solution.
（5）Marine alga bacterium solution compounds：It is mixed with humic acid, urea, potassium dihydrogen phosphate, trace element using marine alga bacterium solution as base fluid
It is even, produce the special marine alga bacterium solution of potato.
2. in step 1）In, sea-tangle 150-300 parts, water 750-1500 parts.
3. in step 1）In, 60-75 DEG C of the temperature of pasteurization, sterilization time 15-20min.
4. in step 2）In, described activation medium is：Beef extract 0.5-1%, yeast extract 0.2-0.5%, peptone
0.1-0.5%, glucose 0,5-1%, NaCl 0.3-0.5%, agar powder 1.5%, 121 DEG C sterilizing 20min after use.
5. in step 2）In, described seed culture medium：Beef extract 0.5-1%, yeast extract 0.2-0.5%, peptone
0.1-0.5%, glucose 0.5-1%, NaCl 0.5%, sodium alginate 2%, agar powder 1.5%, 121 DEG C sterilizing 20min after use.
6. in step 3）In, the fermentation medium：Dregs of beans 1.5-3%, corn steep liquor 0.5-2%, glucose 0.5%-1%,
MgSO4 Used after 0.05-0.1%, 121 DEG C of sterilizing 20min.
7. in step 3）Middle fermentation condition is：Seed inoculum concentration is in 2-5%, and at 25-37 DEG C, speed of agitator exists cultivation temperature
100-300r/min, incubation time is in 10-16h.
8. in step 3）The addition of middle sea-tangle is 2-4%.
9. in step 4）Middle fermentation condition is：Cultivation temperature is at 40-60 DEG C, speed of agitator 200-300r/min, culture
Between in 4-8h.
The remarkable advantage of the present invention：
（1）Marine alga active material is extracted by biological fermentation process, process is gentle, nutritional ingredient free of losses.
（2）The algal polysaccharides yield that the present invention extracts is more than 25%, and viscosity and purity are preferable.
（3）The present invention improves fermentation temperature during fermentation of seaweed, is advantageous to enzyme system caused by microorganism and plays work
With, while stimulate probiotics to form gemma, improve the stability and survival rate of thalline.
Embodiment
Below by example, the present invention is described in further detail, and these examples are only used for illustrating the present invention, not
Limit the scope of the present invention：
Example 1
（1）Pretreatment：150 portions of sea-tangles soak 2h with 750 parts of water, clean up the silt on surface, 1cm mono- is cut into vegetable-chopper
Lower fritter.The marine alga handled well is sterilized 30min under the conditions of 60 DEG C, then cooled down standby.
（2）It is prepared by seed liquor：By bacillus subtilis in activation medium（Containing beef extract 0.5%, yeast extract 0.2%, egg
White peptone 0.1%, glucose 0,5%, NaCl 0.5%, agar powder 1.5%）On activated after, picking single bacterium falls within seed culture medium
（0.5-1% containing beef extract, yeast extract 0.2-0.5%, peptone 0.1-0.5%, glucose 0.5-1%, NaCl 0.5%, alginic acid
Sodium 2%）In be incubated overnight,
（3）The fermentation of probiotics：Seed liquor is added into fermentation medium（Containing dregs of beans 1.5%, corn steep liquor 0.5%, glucose 0.5%,
MgSO4 0.1%）Fermentation tank in, seed liquor addition 3%, 33 DEG C of fermentation temperature, PH ferments 8h under conditions of being 6.5.
（4）Add marine alga：The addition of marine alga is 2%, the marine alga through pasteurization is added in fermentation tank, cultivation temperature
At 45 DEG C, speed of agitator 250r/min, incubation time produces marine alga bacterium solution in 4h.Algal polysaccharides recovery rate 27% after measured.
（5）Potato marine alga bacterium solution compounds：400 parts of marine alga bacterium solution, 15 parts of urea, 10 parts of potassium dihydrogen phosphate, ferrous sulfate 5
Part, 10 parts of zinc sulfate, 5 parts of magnesium sulfate, stir.
Example 2
（1）Marine alga pre-processes：150 portions of marine algas soak 2h with 900 parts of water, clean up the silt on surface, are shredded with vegetable-chopper.Will
The marine alga handled well sterilizes 20min under the conditions of 65 DEG C, then cools down standby.
（2）It is prepared by seed liquor：By bacillus subtilis in activation medium（Containing beef extract 0.5%, yeast extract 0.2%, egg
White peptone 0.1%, glucose 0,5%, NaCl 0.5%, agar powder 1.5%）On activated after, picking single bacterium falls within seed culture medium
（0.5-1% containing beef extract, yeast extract 0.2-0.5%, peptone 0.1-0.5%, glucose 0.5-1%, NaCl 0.5%, alginic acid
Sodium 2%）In be incubated overnight,
（3）The fermentation of probiotics：Seed liquor is added into fermentation medium（Containing dregs of beans 1%, corn steep liquor 1.5%, glucose 0.5%,
MgSO4 0.1%）Fermentation tank in, seed liquor addition 4%, 35 DEG C of fermentation temperature, PH ferments 10h under conditions of being 7.0.
（4）Add marine alga：The addition of marine alga is 3%, the marine alga through pasteurization is added in fermentation tank, cultivation temperature
At 50 DEG C, speed of agitator 3000r/min, incubation time produces marine alga bacterium solution in 6h.Algal polysaccharides recovery rate 26%.
（5）Potato marine alga bacterium solution compounds：300 parts of marine alga bacterium solution, 20 parts of urea, 15 parts of potassium dihydrogen phosphate, ferrous sulfate
10 parts, 15 parts of zinc sulfate, 10 parts of magnesium sulfate, stir.
Example 3
（1）Pretreatment：150 portions of marine algas soak 2h with 1000 parts of water, clean up the silt on surface, are shredded with vegetable-chopper.Will place
The marine alga managed sterilizes 15min under the conditions of 75 DEG C, then cools down standby.
（2）It is prepared by seed liquor：By bacillus subtilis in activation medium（Containing beef extract 0.5%, yeast extract 0.2%, egg
White peptone 0.1%, glucose 0,5%, NaCl 0.5%, agar powder 1.5%）On activated after, picking single bacterium falls within seed culture medium
（0.5-1% containing beef extract, yeast extract 0.2-0.5%, peptone 0.1-0.5%, glucose 0.5-1%, NaCl 0.5%, alginic acid
Sodium 2%）In be incubated overnight.
（3）The fermentation of probiotics：Seed liquor is added into fermentation medium（Containing dregs of beans 1.5%, corn steep liquor 1.5%, glucose
0.5%, MgSO4 0.1%）Fermentation tank in, seed liquor addition 5%, 37 DEG C of fermentation temperature, PH ferments 12h under conditions of being 7.5.
（4）Add marine alga：The addition of marine alga is 5%, the marine alga through pasteurization is added in fermentation tank, cultivation temperature
At 60 DEG C, speed of agitator 3000r/min, incubation time produces marine alga bacterium solution in 10h.Algal polysaccharides recovery rate 28%.
（5）Potato marine alga bacterium solution compounds：500 parts of marine alga bacterium solution, 15 parts of urea, 10 parts of potassium dihydrogen phosphate, ferrous sulfate 5
Part, 10 parts of zinc sulfate, 5 parts of magnesium sulfate, stir.
Common compound fertilizer material is contrasted, above example 1,2,3 and comparative example application are as follows：
Experimental group selects kind of the potato second crop soil of more than 3 years to be tested with control group.Control group uses Common compound fertilizer
Applied fertilizer, experimental group is then applied fertilizer with the special marine alga bacterium solution containing potato.Dose is identical with remaining field management.
Survey data and data analysis such as table：
Using marine alga bacterium solution made from example 1.2.3, the growth characteristics of potato can not only be significantly improved, make plant tall and big, stem
Stalk is sturdy, and branch is more, and leaf area is big, and dry-matter accumulation is vigorous, and can strengthen the disease resistance of potato, improves yield, changes
The quality of kind potato.
Claims (7)
1. a kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation, it is characterized in that, including following technique step
Suddenly：
The first step, marine alga pretreatment：Marine alga is soaked, cleaned, removes the silt on surface, is shredded with vegetable-chopper small to below 1cm
Block, it is then that sea-tangle pasteurization, cooling is standby.
2. second step, prepared by seed liquor：Bacillus subtilis is incubated overnight in seed culture medium.
3. the 3rd step, the fermentation of probiotics：Seed culture medium access liquid fermentation tank is fermented.
4. the 4th step, marine alga throws tank：Sea-tangle through pasteurization is added in fermentation tank, former fermentation temperature is improved, produces marine alga
Bacterium solution.
5. the 5th step, marine alga bacterium solution compounding：Using marine alga bacterium solution as base fluid, with humic acid, urea, potassium dihydrogen phosphate, trace element
Mix, produce the special marine alga bacterium solution of potato.
6. sea-tangle pretreatment as claimed in claim 1, it is characterised in that sea-tangle 150-300 parts, water 750-1500 parts；Such as right
It is required that the 1 sea-tangle pretreatment, it is characterised in that 60-75 DEG C of the temperature of pasteurization, sterilization time 15-20min；Such as right
It is required that described in 1, bacillus subtilis is the excellent species that domestication is screened by our company, the hypersaline environment in suitable fermentation of seaweed；Such as
The fermentation of bacillus subtilis described in claim 1, it is characterised in that the fermentation medium：Dregs of beans 1.5-3%, corn steep liquor
0.5-2%, glucose 0.5%-1%, MgSO4 Used after 0.05-0.1%, 121 DEG C of sterilizing 20min；Withered grass as claimed in claim 1
The fermentation of bacillus, it is characterised in that fermentation condition is：Seed inoculum concentration is in 2-5%, and at 25-37 DEG C, stirring turns cultivation temperature
Speed throws tank in 100-300r/min, incubation time in 10-16h. sea-tangles as claimed in claim 1, it is characterised in that:Sea-tangle adds
Dosage is 2-4%.
7. sea-tangle as claimed in claim 1 throws tank, it is characterised in that fermentation condition is：Cultivation temperature is at 40-60 DEG C, speed of agitator
200-300r/min, incubation time is in 4-8h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710194858.XA CN107619336A (en) | 2017-03-29 | 2017-03-29 | A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710194858.XA CN107619336A (en) | 2017-03-29 | 2017-03-29 | A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107619336A true CN107619336A (en) | 2018-01-23 |
Family
ID=61088003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710194858.XA Pending CN107619336A (en) | 2017-03-29 | 2017-03-29 | A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107619336A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467495A (en) * | 2019-08-26 | 2019-11-19 | 江西省水产技术推广站 | A kind of water-soluble composition and its preparation method and application adjusting algal grown |
| CN111034994A (en) * | 2019-11-18 | 2020-04-21 | 福建卫生职业技术学院 | Preparation method of functional fermented laminarin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006320257A (en) * | 2005-05-19 | 2006-11-30 | Grandhill Osaka:Kk | Seaweed fermented extract, method for producing the extract, and usage of the extract |
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN103304278A (en) * | 2012-11-06 | 2013-09-18 | 青岛明月海藻集团有限公司 | Seaweed fertilizer comprising seaweed leach liquor and preparation method thereof |
| CN105367253A (en) * | 2015-11-28 | 2016-03-02 | 王德和 | Bacterial wilt resistant and drought resistant fertilizer for potatoes |
| CN106518278A (en) * | 2016-09-27 | 2017-03-22 | 日照益康有机农业科技发展有限公司 | Selenium enriched algae bioorganic fertilizer and preparation method thereof |
-
2017
- 2017-03-29 CN CN201710194858.XA patent/CN107619336A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006320257A (en) * | 2005-05-19 | 2006-11-30 | Grandhill Osaka:Kk | Seaweed fermented extract, method for producing the extract, and usage of the extract |
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN103304278A (en) * | 2012-11-06 | 2013-09-18 | 青岛明月海藻集团有限公司 | Seaweed fertilizer comprising seaweed leach liquor and preparation method thereof |
| CN105367253A (en) * | 2015-11-28 | 2016-03-02 | 王德和 | Bacterial wilt resistant and drought resistant fertilizer for potatoes |
| CN106518278A (en) * | 2016-09-27 | 2017-03-22 | 日照益康有机农业科技发展有限公司 | Selenium enriched algae bioorganic fertilizer and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467495A (en) * | 2019-08-26 | 2019-11-19 | 江西省水产技术推广站 | A kind of water-soluble composition and its preparation method and application adjusting algal grown |
| CN111034994A (en) * | 2019-11-18 | 2020-04-21 | 福建卫生职业技术学院 | Preparation method of functional fermented laminarin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101941851B (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| AU2008331378B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN105684727B (en) | The culture medium and cultural method for the black collybia albuminosa of cultivation expected based on ferment bacteria residue | |
| CN102344326B (en) | Organic biofertilizer capable of inhibiting continuous cropping obstacle of flue-cured tobaccos | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN102924156A (en) | Culture medium and cultural method for cultivating coprinus comatus | |
| CN106701621A (en) | Straw decomposing additive for decomposition in low-temperature state and preparation method of straw decomposing additive | |
| CN107794240A (en) | One plant of bacillus megaterium, its polypeptide agricultural microbial agent and preparation method and application | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN102173886A (en) | Oyster mushroom culture medium as well as fermentation inoculum and application thereof | |
| CN103621316B (en) | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN106916021A (en) | A kind of double source humic acid biological fertilizer and preparation method and application | |
| CN103626553A (en) | Method utilizing sunflower by-products to manufacture white fungus cultivation material | |
| CN105948831B (en) | A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains | |
| CN105198556A (en) | Microbial fertilizer capable of improving sweetness of oranges and preparation method thereof | |
| CN108633625A (en) | The method for preparing White mushroom cultivation base as primary raw material using Pleurotus eryngii mushroom bran | |
| CN112205274A (en) | Rice seedling raising matrix based on various agricultural wastes and preparation method thereof | |
| CN105439726A (en) | Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof | |
| CN101870595B (en) | Old ginseng land soil biological modifying agent | |
| CN107619336A (en) | A kind of method that the special marine alga bacterium solution of potato is prepared using microbial fermentation | |
| CN103704018B (en) | Method for preparing pleurotus salmoneostramineus liquid spawns with yellow serofluid as principal raw materials | |
| CN104844285A (en) | Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180123 |